Specialty Spring Barley Variety and Nitrogen Rate Trials across Northeastern Oregon Don Wysocki, Darrin L. Walenta, Mike Flowers, Nick D.

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Specialty Spring Barley Variety and Nitrogen Rate Trials across Northeastern Oregon Don Wysocki, Darrin L. Walenta, Mike Flowers, Nick D. Sirovatka Abstract Five varieties of spring barley, Camas, Baronesse, Merlin, Salute, and YU599-06 were grown in locations in Union and Wallowa Counties in 2007, 2008, and 2009. Camas and Baronesse are commonly grown feed barley cultivars. Merlin, Salute and YU599-06 are waxy lines that have high beta glucans. Yields of feed types were compared to waxy lines. Under dryland conditions Merlin and Salute yield similar to Camas and Baronesse. Under irrigation all lines yielded similar to feed barley. Higher rates of nitrogen above the grower s customary rate did generally increase yield and often reduced test weight. Overall the most adopted lines of waxy barley will yield equal to feed barley. However, specific performance of waxy cultivars was different under irrigated and dryland management. Keywords: feed barley, nitrogen, waxy barley Introduction Barley is the world's fourth largest cereal crop. In Oregon, barley has traditionally been grown as a feed grain or for malting purposes. Feed barley is second to wheat as the most commonly grown small grain in Oregon. The acreage of feed barley fluctuates with market prices. In dryland areas, feed barley has been the most dominant rotation crop with wheat. Malting barley, though higher in price, has been grown to a lesser extent because of a much smaller market, the need to contract acres, and the requirements for meeting plumpness and protein standards. Over the past several years there has been increased interest in food barley. Food barley has waxy starch that is high in soluble dietary fiber, especially beta-glucans. Human consumption of soluble fiber from barley is an effective way of lowering serum cholesterol (Ames et al. 2006; Ames and Rhymer 2008). One proposed use of waxy barley grown in the region would be fractionation to produce soluble fiber to provide ingredients in various food products. Less valuable fractions would be converted to ethanol by either fermentation or gasification to be blended with gasoline for liquid motor fuel. Thus waxy type barley due to its health benefits and use as a human food would have a higher market price compared to feed barley. Although much is known about feed and malting barley, very little is known about the performance and management of waxy barley in northeast Oregon. Waxy cultivars have not been routinely grown in variety trials so little information is available to growers. In addition, waxy cultivars may respond differently to applied nitrogen. Two critical production needs for growers are the evaluation of available waxy cultivars and better understanding of management of nitrogen for yield and quality characteristics. Norberg et al. (2009) investigated irrigated production of waxy barley cultivars. They studied irrigated production of fall-planted springtype waxy barley cultivars coupled with nitrogen application and use of plant growth regulators

to control lodging. Rey et al (2009) studied waxy barley in dryland conditions in Pendleton. They demonstrated the need to manage nitrogen for quality and agronomic performance. The purpose of our trials was to determine the response of nitrogen on spring sown waxy barley in dryland and irrigated conditions in Union and Wallowa counties. Performance of waxy barley was compared to commonly grown feed barley. Materials and Methods We established trials in Union and Wallowa Counties in 2007, 2008, and 2009 to evaluate the agronomic performance and grain quality of three waxy barleys and two widely grown spring feed barleys (Table 1). Trials were conducted under dryland conditions in Union County and under irrigation in Wallowa County (Table 2). To investigate N fertilizer interactions with grain yield, two nitrogen fertilizer rates were applied to each variety. A customary rate (rate used by the grower for feed barley) and a high (usually an additional 25 lb/acre) rate were used to determine if increased nitrogen would improve protein and beta glucan content. Sowing date of the trials varied from April to May depending on location, year and weather conditions (Table 3). Prior to planting, plot areas were prepared by grower cooperators with an application of glyphosate and each grower s residue management operation. Plots were sown in a randomized complete block design with four replications using a Fabro direct seed plot drill with Atom jet C- shank openers on 12-inch centers (Figure 1). Nominal planting depth was 1 inch. Cooperators supplied the tractor to pull the drill. Fifty pounds of granular 16-20-0-14 were placed with the seed and additional N to reach the treatment level was placed 2 inches below and to side of the seed as dry 46-0-0 through disk applicators on the drill. Varieties were sown at a rate of 25 seed/ ft 2 (Table 1). Plots were harvested with a Hege 140 plot combine with de-awning bars installed in the concave. Yields were determined from harvest weights and plot dimensions. Test weight on barley grain was determined. Quality characteristics of percent protein, waxy starch, and beta glucan are being determined in the laboratory. This paper reports only yield and test weight results. Table 1. Barley varieties and characteristics. Variety Description Origin Seeds/lb Sowing rate lb/acre @ 25 seed/ft 2 Baronesse Hulled, non-waxy, 2-row, WestBred 10,485 104 feed barley Camas Hulled, non-waxy, 2 row, Univ. Idaho 11,611 94 feed barley Merlin Hull-less, waxy, 2-row WestBred 10,879 100 barley Salute Hulled, waxy, 2-row, high WestBred 9,271 117 beta glucan YU 599-06 Hulled, waxy, 6 row, high beta glucan WestBred 9,863 110

Table 2. Cooperators and N rates used in the studies Year Cooperator Nitrogen Fertility Treatments (lb/acre) Customary High+ Union County (dryland) 2007 Elwyn Bingaman 100 130 2008 TRICO Farms 75 100 2009 TRICO Farms 75 100 Wallowa County (irrigated) 2007 Alan Klages 100 130 2008 Melville Farms 93 118 2009 Melville Farms 93 118 Table 3. Soil test values and planting and harvest dates of trials. Year Location ph Available Nitrogen* lb/acre Phosphorus (ppm) Sulfur (ppm) Planting Date Harvest Date Union County 2007 Bingaman -- -- -- -- 4/13 8/7 2008 Trico 6.8 104 38 14.9 4/10 8/8 2009 Trico 7.1 156 33 15.9 4/8 8/4 Wallowa County 2007 Klages -- -- -- -- 5/4 8/30 2008 Melville 6.0 189 35 21.6 4/19 9/8 2009 Melville 6.3 166 24 25.5 4/17 8/24

Figure 1. Fabro drill sowing trial TRICO Farms 2008 Results Union County Varieties responded differently between Union and Wallowa Counties, primarily because of irrigation. Due to this difference, each county s sites will be discussed individually. Yield and test weight results for Union County are shown in Table 4. In Union County, yields were similar in 2007 and 2008, but were much higher in 2009. Trial averages were 2,385, 2,778, and 4,940 respectively for 2007, 2008, and 2009. The lowest yield was Merlin at the high nitrogen rate in 2007 and highest yield was Baronesses at the high nitrogen rate in 2009 (Table 4). Test weights were highest in 2009, lowest in 2007 and intermediate in 2008. There were large differences in test weight from year to year. This may be due to weather conditions or location. Precipitation at the National Weather Service site at La Grande for crop years (September-July) for 2007, 2008 and 2009 was 16.42, 15.33 and 17.89 inches respectively. Comparing yields of feed barley lines to waxy lines, Salute performed as well as the feed lines in all three years of the study in Union County. Merlin performed equal to the feed barley lines in two of three years and YU599 never yielded as well. Over the course of the study, additional nitrogen usually resulted in equal or lower yield and equal or lower and significantly reduced test weight. We suspect that this is due to high residual soil nitrogen levels at planting (Table 3). Test weights for Merlin were significantly different than other varieties. Merlin is a hull-less type so this was expected and cannot be used for comparison. Comparing the hulled types, YU599-06 consistently had lower tests weights than other waxy or feed cultivars.

Table 4. Yield and test weight, Union County trials, 2007, 2008, 2009. 2007 2008 2009 Average Yield Test wt. Yield Test wt. Yield Test wt. Yield Test wt. Lb/a Lb/bu Lb/a Lb/bu Lb/a Lb/bu Lb/a Lb/bu Baronesse 2388abc 50.6de 3129a 43.4ab 4972ab 54.1c 3496abc 49.4ab Baronesse* 2738a 50.2ef 2677abc 40.7bc 5464a 54.0 3626a 48.3bc Camas 2706a 52.2c 3097a 45.1a 4703ab 54.7 3502abc 50.7ab Camas* 2499ab 51.1cde 2943ab 43.8ab 5034ab 54.5 3492agc 49.8ab Merlin 2180bc 56.8a 2633abc 40.5bc 4721ab 60.6 3178bcd 52.6a Merlin* 1962c 54.9b 2298c 37.6c 4639b 59.2 2966d 50.6ab Salute 2840a 51.8cd 3036a 44.1ab 4810ab 54.5 3562ab 50.1ab Salute* 2380abc 50.6de 2889abc 43.7ab 5088ab 54.2 3452abc 49.5ab Yu599-06 2112bc 48.9fg 2745abc 37.1c 4962ab 49.3 3254abc 45.1c d Yu599-06* 2045bc 48.3g 2348bc 38.1c 5044ab 48.9 3146cd 45.1c LSD 0.05 471 1.6 627 4.3 765 1.1 384 4.2 *= high N rate Wallowa County Yield and test weight for irrigated trials in Wallowa County are shown in Table 5. Overall yields at the Klages site in 2007 were lower than at the Melville sites in 2008 and 2009. In 2007, the trial was planted later in the spring and the crop was less competitive with weeds. Trial averages were 4093, 5532, and 5818 lb/acre respectively for 2007, 2008, and 2009. All sites had wheel line irrigation. The lowest yield was YU599-06 at the customary nitrogen rate in 2007 and highest yield was YU599-06 at the high nitrogen rate in 2009 (Table 5). As might be expected, test weights under irrigated conditions in Wallowa County were more consistent. Overall, test weights were highest in 2007, lowest in 2009 and intermediate in 2008, although they typically varied at less than a pound per bushel. Salute and Merlin (waxy types) yielded as well or better than feed barley cultivars in all three years. Yield of YU599-06 was less consistent. In comparison to the feed barley cultivars, it yielded similar in 2007, lower in 2008 and superior in 2009. The effect of additional nitrogen was inconsistent among years and varieties in regard to yield. In 2007, the higher nitrogen increased yields of Baronesse, Salute, and YU599. In 2008 and 2009 Baronesse and YU599 showed increased yield with the added nitrogen. Test weights tended to remain the same or to decrease slightly with added nitrogen. Discussion Union County Results of this study varied between dryland sites in Union County and irrigated sites in Wallowa County. Waxy barley varieties performed differently in these two management systems (Tables 4 and 5). Under dryland conditions in Union County, the waxy cultivars, Merlin and Salute yielded similar to Camas and Baronesse, with Merlin yielding equal in all three years and Salute yielding equal in two of three years. However, YU599-06 consistently yielded less over the duration of the study. Because Merlin is hulless barley, its test weight cannot be

directly compared with the hulled types. Test weights of Salute consistently equaled those of the Camas and Baronesse. Test weights of YU599 were always lower than the other lines. Additional levels of nitrogen above the grower s customary rate generally resulted in equal or lower yields and usually lower test weight. Neither feed barley nor waxy barley responded to additional nitrogen. Soil test results (Table 3) showed the background levels of nitrogen were high. These levels of soil nitrogen suggest nitrogen can be used more judiciously in most years. Wallowa County Waxy barley lines responded differently under irrigation (Table 5). Merlin and Salute yielded similar to Camas and Baronesse in all three years of this study. YU599 yielded equal to or better than the feed barleys in 2007 and 2009, but less in 2008. Test weight of Salute where similar to test weights of Camas and Baronesse. In comparison, test weight of YU599 was consistently lower. The high nitrogen rates showed inconsistent results among years and varieties. In 2007 Baronesse, Salute and YU599 showed increased yield. Baronesse and YU599 showed this in 2008 and 2009. However, the difference in yields between the high and customary rates is on the order of 200-300 lb/acre. At the current cost of N this amount of yield would not cover the cost of the additional N. Test weights did not decline with additional nitrogen, except for Camas, which had lower test weight in 2008 and 2009. Table 5. Wallowa County Trials (Irrigated) 2007 2008 2009 Average Yield Test wt. Yield Test wt. Yield Test wt. Yield Test wt. Variety lb/acre lb/bu lb/acre lb/bu lb/acre (lb/bu) (lb/acre) (lb/bu) Baronesse 4410 ab 51.5 cd 5369 bc 51.2 bc 5765 c 48.7 cd 5182 ab 50.5 b Baronesse* 4711 ab 52.7 bc 5501 abc 50.8 c 5697 c 48.1 d 5303 ab 50.5 b Camas 4920 a 52.7 bc 5752 ab 52.3 b 5906 c 50.2 bc 5526 a 51.7 b Camas* 4094 ab 52.7 bc 5536 abc 51.3bc 5286 de 47.6 de 4972 ab 50.6 b Merlin 4148 ab 58.2 a 5658 abc 55.6 a 5701 c 52.4 a 5169 ab 55.4 a Merlin* 3840 ab 58.4 a 5731 ab 55.4 a 5706 c 51.1 ab 5092 ab 55.0 a Salute 3462 b 50.5 de 5165 cd 51.3bc 5580 cd 48.6 cd 4736 b 50.1 b Salute* 4036 ab 53.1 bc 4850 d 50.9 c 5178 e 47.7 de 4688 b 50.6 b YU599-06 3519 b 49.8 e 5809 ab 46.4 d 6403 b 45.3 f 5244 ab 47.2 c YU599-06* 3788 ab 49.1 e 5952 a 46.1d 6962 a 45.9 ef 5568 a 47.2 c LSD (0.05) 556 1.5 518 1.3 180 2.1 722 1.8 * denotes high N rate Conclusions and Recommendations Based on yields and test weights results of this study under both irrigated and dryland conditions we have the following conclusions and recommendations for growing waxy type barley cultivars.

1. Under dryland production, Salute and Merlin yield similar to Camas and Baronesse. Growers can expect similar yields with these lines. YU did not perform as well as the other waxy cultivars. 2. Under irrigated production all waxy lines performed equal to feed barley and Merlin and YU599, respectively, yield better in 2008 and 2009, respectively, than the feed barley cultivars. 3. Rates of nitrogen above the grower s customary rate are not warranted under either dryland or irrigated yields, where soil test levels are high. In this study, the results indicate that for waxy barley cultivars it is not necessary to increase N-rates to achieve similar grain yield obtained with standard feed-type cultivars. Growers can probably reduce customary rates of N by using timely soil tests. However, quality characteristics such beta glucan content or protein level may be are factors that improve with additional nitrogen. This study indicates that adopted cultivars of waxy barley can yield similar to commonly grown feed barley cultivars. If there is an available market and higher or equal prices for waxy, growers could expect incomes higher or similar to feed barley. If waxy barley commands a higher price then growers can expect yields similar to feed barley and can budget based on historic feed barley yields and expected price. Acknowledgement We appreciate the support of the Oregon Grains Commission for providing funding and the following grower/ cooperators for providing vital resources (land, equipment, etc.) necessary for completion of the project Literature Cited Ames N., C. Rhymer, B. Rossnagel, M. Therrien, D. Ryland, S. Dua, and K. Ross. 2006. Utilization of diverse hulless barley properties to maximize food product quality. Cereal Foods World. Ames, N. P and C.R. Rhymer. 2008. Issues Surrounding Health Claims for Barley J. Nutr. 138: 1237S 1243S. Norberg S., B Brown, C. Shock, A. Ross, P. Hayes, and J. Rey. Waxy Barley: Optimizing Nitrogen Use for Yield and Grain Quality. ASA Abstracts. http://a-cs.confex.com/crops/2009am/webprogram/paper53655.html verified March 30 2009 Rey, J.I., P.M. Hayes, S.E. Petrie, A. Corey, M. Flowers, J.B. Ohm, C. Ong, K. Rhinhart, and A.S. Ross. 2009. Production of Dryland Barley for Human Food: Quality and Agronomic Performance. Crop Sci. 49:347-355