Department of Microbiology 3 rd Floor Darent Valley Hospital Darenth Wood Road Dartford DA2 8DA United Kingdom Contact: Mr Kurt Djemal Tel: +44 (0) 1322 428733 E-Mail: k.djemal@nhs.net Website: http://www.dvh.nhs.uk/ Testing performed at the above address only DETAIL OF ACCREDITATION Swabs (noise, groin, throat, wound) and urine Sputum, bronchoalveolar lavage, and brushings, cerebrospinal fluid, pus, swabs, blood, bone marrow, urine, tissues and biopsies, swabs (throat, ear, nose, eye, wound, pernasal), bile, CSF, genital tract specimens, sterile body fluids, pus, tissues and biopsies, sputum, bronchoalveolar lavage, and brushings, swabs, blood, bone marrow MRSA Isolation and presumptive identification of acid-alcohol fast bacilli (inc. Mycobacterium species) significance instructions where relevant Manual culture using prepared manufacturers chromogenic agar plates Microbiology 7216 Investigation of MRSA screening swabs based on PHE SMI B 29 BacT/Alert 3D using MP and MB liquid growth media Microbiology 6000 Investigation of specimens for Mycobacterium species based on PHE SMI B 40 Manual culture using prepared manufacturers agar plates including chromogenic media Manual gram stain Assessment Manager: HL Page 1 of 7
, swabs (throat, ear, nose, eye, wound, pernasal), bile, CSF, genital tract specimens, sterile body fluids, pus, tissues and biopsies, sputum, bronchoalveolar lavage, and brushings, swabs, blood, bone marrow. significance instructions where relevant Microbiology 7236 Investigation of genital tract and associated specimens based on PHE SMI B 28 Microbiology 7369 Antimicrobial susceptibility Testing using disc diffusion following BSAC guidelines Microbiology 7218 Investigation of sputum and associated specimens based on PHE SMI B 57 Microbiology 7220 Investigation of faeces based on PHE SMI B 30. Microbiology 7278 Investigation of throat swabs based on PHE SMI B 9 Microbiology 7275 Investigation of ear swabs based on PHE SMI B 1Microbiology 7277 Investigation of nose swabs based on PHE SMI B 5 Microbiology 7275 Investigation of eye swabs based on PHE SMI B 2 Microbiology 7279 Investigation of specimens for Bordetella species based on PHE SMI 6 Microbiology 7280 Investigation of wound swabs and associated specimens based on PHE SMI B 11, 14 and 17 Microbiology 415 Investigation of bile based on PHE SMI B 15 Microbiology 7282 Investigation of sterile fluids based on PHE SMI B 26 Assessment Manager: HL Page 2 of 7
, swabs (throat, ear, nose, eye, wound, pernasal), bile, CSF, genital tract specimens, sterile body fluids, pus, tissues and biopsies, sputum, bronchoalveolar lavage, and brushings, swabs, blood, bone marrow Whole blood Cultures grown in house and directly from positive blood cultures from the above Antibiotic susceptibility testing of bacteria of clinical significance significance and Antibiotic susceptibility testing of bacteria of clinical significance Identification of clinically significant bacteria and yeasts instructions where relevant. Antimicrobial susceptibility testing using disc diffusion following BSAC guidelines Microbiology 7367 Investigation of blood cultures based on PHE SMI B 37 using BacT/Alert (liquid culture) Use of Grams stain and API system. AST disc diffusion following BSAC guidelines supplemented by gradient MIC tests where necessary. SOP.MIC.9 Use of the MLADITOF using Automated Matrix-assisted laser desorption/ionisation time of flight mass spectrophotometer (MALDITOF) Detection of Helicobacter pylori antigen Detection of Clostridium difficile toxin and Clostridium difficile GDH Microbiology 611 Detection of Helicobacter pylori antigen in faeces based on PHE SMI B 55 using DS2 by EIA Microbiology 7371 Detection of Clostridium difficile toxin in faeces based on PHE SMI B 10 using DS2 by EIA Assessment Manager: HL Page 3 of 7
Urine Detection and identification of helminths, nematodes, flukes, parasitic larvae, protozoa, ova, eggs, ectoparasites, and cysts of clinical significance Detection, identification and quantification of red cells, white cells, epithelial cells, microorganisms and parasites of clinical significance instructions where relevant. Using faecal concentration method followed by microscopy Microbiology 7224 Investigation of parasites based on PHE SMI 31 Microbiology 7220 Investigation of faeces based on PHE SMI B 30 Automated screening using Menarini Sedimax. Key SOPs: Microbiology 7368 Investigation of urine Microbiology 1633 urine microscopy Microbiology 1677 urine culture using Menarini SediMAX and Mast UriPlus based on PHE SMI B 41 Assessment Manager: HL Page 4 of 7
Urine Serum significance and Antibiotic susceptibility testing of bacteria of clinical significance Serology examination activities for Detection of the following viral antigens/antibodies: instructions where relevant. Semi-automated culture using Mast UriPlus. Manual culture using prepared manufacturers agar plates including chromogenic media Manual gram stain Antimicrobial susceptibility testing using disc diffusion following BSAC guidelines Key SOPs: Microbiology 7368 Investigation of urine Microbiology 1677 urine culture using Menarini SediMAX and Mast UriPlus based on PHE SMI B 41 Antimicrobial susceptibility testing. ELISA using DS2 Hepatitis B surface antigen HBsAg: Microbiology 751 2 Hepatitis B antibody Anti HBs: Microbiology 755 Hepatitis C antigen/antibody HIV antigen/antibody Hepatitis C antigen/antibody: Microbiology 754 HIV antigen/antibody: Microbiology 752 2 Assessment Manager: HL Page 5 of 7
Serum Serum/plasma Serum/plasma Serology examination activities for Detection of the following viral antigens/antibodies: Rubella IgG antibody Syphilis total antibodies Rheumatoid IgM antibody Detection of Hepatitis B surface antigen Hepatitis B core total Rubella IgM antibody Hepatitis A IgG and IgM antibodies EBV IgG and IgM antibodies VZV IgG antibody CMV IgG and IgM antibodies Toxoplasma (competition) HIV 1/2 confirmation Measles IgG Detection of serum antibodies to HIV1/2 for confirmation ELISA using DS2 Rubella IgG antibody: Microbiology 756 Syphilis total antibodies: Microbiology 753 Rheumatoid IgM antibody: Microbiology 757 ELFA using VIDAS Microbiology 349 using Biomerieux VIKIA. Microbiology 785 Serum Detection of Mycoplasma IgM Manual EIA Immunocard using Meridian Immunocard Microbiology 1204 Serum Nasopharyngeal aspirate Urine Qualitative and semiquantitative determination of ASO Detection of RSV antigens (fusion protein or nucleoprotein) Detection of Legionella pneumophila serogroup 1 antigen Manual Latex agglutination using Biokit Rheumoject Microbiology 7370 using Meridian TRU Microbiology 3077 using BinaxNow Legionella kit Microbiology 7373 and SOP.MIC.3 Assessment Manager: HL Page 6 of 7
Serology examination activities for Urine Detection of pneumococcal antigen. using BinaxNow Strep pneumoniae kit Microbiology 7373 and SOP.MIC.3 Urine and genital swabs Qualitative determination of rotaviruses and/or adenoviruses Molecular examination activities for Detection of Chlamydia trachomatis DNA Molecular tests without pathologist opinion or interpretation using Biopharm Ridaquick Adeno/ Rota Combi Microbiology 7377 Microbiology 7200 using Tecan and Taqman by PCR Lymph node tissue Detection of cytokeratin 19 Microbiology 1519 using RD-100i by one step nucleic acid amplification (OSNA) Miscellaneous tests Joint fluids Detection and identification of birefringent crystals Manual polarising microscopy Microbiology 7282 Investigation of sterile fluids based on PHE SMI B 26 END Assessment Manager: HL Page 7 of 7