LightCycler EvoScript RNA Masters Datasheet

LightCycler EvoScript RNA Masters Datasheet

Witness the evolution of one-step RT-qPCR Extracting reliable answers from RNA can be a challenge. When samples are poor and copy numbers are low, rely on the next generation of master mixes from the pioneer in PCR technology. Trust one-step EvoScript RNA Masters from Roche. Designed for gene expression and pathogen detection applications on your LightCycler System, EvoScript RNA Masters deliver unprecedented performance, unmatched ease-of-use and measurably better results when compared with the SuperScript III Platinum One-Step kits.* Superior performance* Highly sensitive detection, down to nearly one copy Detects rare transcripts for gene expression applications Broad dynamic range for superior quantification 5X master mix concentration for higher sensitivity, even with larger sample volumes Superior data quality* Highly thermostable reverse transcriptase for consistent, stable results, including tolerance of targets of varying secondary structure Works on RNA targets isolated from a variety of sample materials UNG compatible for contamination control capabilities Superior ease-of-use* Single-vial formulation for convenience, speed, and reduced errors Superior specificity for easier assay optimization Hot-start protocol and a single enzyme for reverse transcription and polymerase chain reaction, to obtain faster, more specific results *Based on the results of head-to-head tests of the LightCycler EvoScript RNA SYBR Green I Master versus the SuperScript III Platinum SYBR Green One-Step qrt-pcr Kit and the LightCycler EvoScript RNA Probes Master versus SuperScript III Platinum One-Step qrt-pcr Kit. Tests were run with various sample materials using the optimal conditions specified in the instructions for use for all products. Roche EvoScript 2

Examine the evidence Whether for translational research or lab developed tests, LightCycler EvoScript RNA Masters set the standard for RT-qPCR. Transcribing, amplifying and detecting faster and more efficiently than other leading mixes. In head-to-head comparisons with SuperScript III, LightCycler EvoScript RNA Masters from Roche delivered measurably better sensitivity, specificity and performance. LightCycler EvoScript RNA SYBR Green I Master 2.000 Performance Over a Dynamic Range 1.800 1.600 1.400 1.200 Fluorescence 1.000 0.800 0.600 0.400 0.200 0.000 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 42.00 44.00 Ten-fold dilutions of HER3 Transcripts were amplified using the LightCycler EvoScript RNA SYBR Green I Master. The assay was run on a LightCycler 96 instrument. Cycle Roche EvoScript 3

LightCycler EvoScript RNA SYBR Green I Master (continued) 18 Earlier Cq Values, Higher Florescence, Higher Percentage PCR Efficiency 16 14 LC EvoScript SYBR YWHAZ 10ng/rxn Avg Cq 19.9 LC EvoScript SYBR YWHAZ 1ng/rxn Avg Cq 23.2 LC EvoScript SYBR YWHAZ 0.1ng/rxn Avg Cq 26.6 LC EvoScript SYBR YWHAZ NTC 12 SSIII SYBR YWHAZ 10ng/rxn Avg Cq 20.2 SSIII SYBR YWHAZ 1ng/rxn Avg Cq 23.0 SSIII SYBR YWHAZ 0.1ng/rxn Avg Cq 23.8 SSIII SYBR YWHAZ NTC 10 RFI 8 6 4 2 0 0 5 10 15 20 25 30 35 40 45 Cycles LightCycler EvoScript RNA SYBR Green I Master (blue) and SuperScript III Platinum SYBR Green One-Step qrt-pcr Kit (red) were used to amplify the YWHAZ gene at three concentrations (10ng/reaction, 1ng/reaction and 0.1ng/reaction). Reactions were performed on a LightCycler 480 instrument. Compatible with FFPET Samples Comparable with Other Mixes 30 25 LC EvoScript SYBR GusB FFPET 20ng/rxn Avg 29.2 LC NGOS SM GusB NTC SSIII SYBR GusB FFPET 20ng/rxn Avg 28.0 SSIII SYBR GusB NTC 20 15 RFI 10 5 0 0 5 10 15 20 25 30 35 40 45 Cycles LightCycler EvoScript RNA SYBR Green I Master (blue) and SuperScript III Platinum SYBR Green One-Step qrt-pcr Kit (red) were used to amplify the GusB gene from Formalin-fixed Paraffin Embedded Tissue samples on a LightCycler 480 instrument. Roche EvoScript 4

LightCycler EvoScript RNA Probes Master 8.000 Performance Over a Dynamic Range 7.000 6.000 5.000 Fluorescence (465-510) 4.000 3.000 2.000 1.000 0.000 2.00 4.00 6.00 8.00 10.00 12.00 14.00 16.00 18.00 20.00 22.00 24.00 26.00 28.00 30.00 32.00 34.00 36.00 38.00 40.00 42.00 44.00 Cycle Ten-fold dilutions of HER3 Transcripts were amplified using the LightCycler EvoScript RNA Probes Master. The assay was run on a LightCycler 480 instrument. Earlier Cq Values, Higher Florescence, Higher Percentage PCR Efficiency 14 12 LC EvoScript Probes PGBD SSIII Probes PGBD 10 RFI 8 6 4 2 0 0 5 10 15 20 25 30 35 40 45 Cycles LightCycler EvoScript RNA Probes Master (blue) and SuperScript III Platinum One-Step qrt-pcr Kit (red) were used to amplify the PGBD gene at three concentrations (10ng/reaction, 1ng/reaction and 0.1ng/reaction). Reactions were performed on a LightCycler 480 instrument. Roche EvoScript 5

LightCycler EvoScript RNA Probes Master (continued) 9 Compatible with FFPET Samples Outperforming Other Mixes 8 LC EvoScript Probes PGBD1 SSIII Probes PGBD1 7 6 5 RFI 4 3 2 1 0 0 5 10 15 20 25 30 35 40 45 Cycles LightCycler EvoScript RNA Probes Master (blue) and SuperScript III Platinum One-Step qrt-pcr Kit (red) were used to amplify the PGBD1 gene from Formalin-fixed Paraffin Embedded Tissue samples on a LightCycler 480 instrument. The EvoScript formulation Formulated to meet your gene expression and pathogen detection needs, LightCycler EvoScript RNA Masters deliver outstanding performance for faster RNA workflows and more consistent results. Single-vial format 5X RT-qPCR Master Mix Novel reverse transcriptase enzyme + FastStart Taq DNA Polymerase Blend Mg 2+ as co-factor Stable at room temperature for up to 4 hours Roche EvoScript 6

Demand the truth. Demand EvoScript. EvoScript Reverse Transription products from Roche are available in convenient order quantities for use on your LightCycler System and non-roche instruments. Order EvoScript RNA Masters LightCycler EvoScript RNA Probes Master 07 800 029 001 (200 reactions) LightCycler EvoScript RNA Probes Master 07 800 096 001 (1,000 reactions) LightCycler EvoScript RNA SYBR Green Master 07 800 134 001 (200 reactions) LightCycler EvoScript RNA SYBR Green Master 07 800 177 001 (1,000 reactions of 20) READY TO EvoScript? Get more details and place your order today GET Order EvoScript RNA Masters for non-roche qpcr instruments EvoScript RNA Probes Master EvoScript RNA Probes Master (ROX) EvoScript RNA SYBR Green Master EvoScript RNA SYBR Green Master (ROX) 07 912 269 001 (200 reactions) 07 912 293 001 (200 reactions) 07 912 480 001 (200 reactions) 07 912 498 001 (200 reactions) Also available for two-step RT-PCR EvoScript Universal cdna Master EvoScript Universal cdna Master EvoScript Universal cdna Master EvoScript Reverse Transcriptase EvoScript Reverse Transcriptase 07 912 374 001 (50 reactions) 07 912 439 001 (100 reactions) 07 912 455 001 (200 reactions) 07 912 315 001 (500 units) 07 912 323 001 (2,000 units) Roche EvoScript 7

Published by Roche Molecular Diagnostics 4300 Hacienda Drive Pleasanton, CA 94588 USA ldtsolution.roche.com 2016 Roche Diagnostics. Roche EvoScript 8