Bacterial Antibiotic Resistance from Chapter 9 Microbiology: A Systems Approach 1 st Edition Cowan & Talaro
Types of intermicrobial exchange conjugation transformation requires the attachment of two related species & formation of a bridge that can transport DNA transfer of naked DNA transduction DNA transfer mediated by bacterial virus 2
conjugation 3
(a, b) Process of Conjugation (c) F Factor Transfer Bridge Donor Recipient F + F (d) Hfr Transfer Donor Recipient Pilus Hfr cell Integration of into chromosome Pilus Chromosome Chromosome F + F Partial copy of donor chromosome (a) The pilus of donor cell (top) attaches to receptor on recipient cell and retracts to draw the two cells together. being copied F + F + Bridge broken Donated genes Conjugation bridge (b) An opening or pore forms between the cell walls, thereby creating a bridge to transmit genetic material. (c) Transfer of the, or conjugative plasmid. A cell must have this plasmid to transfer chromosomal genes. (d) High frequency (Hfr) transfer involves transmission of chromosomal genes from a donor cell to a recipient cell. The donor chromosome is duplicated and transmitted in part to a recipient cell, where 4 it is integrated into the chromosome.
(a, b) Process of Conjugation Pilus (a) The pilus of donor cell (top) attaches to receptor on recipient cell and retracts to draw the two cells together. 5
(a, b) Process of Conjugation Pilus (a) The pilus of donor cell (top) attaches to receptor on recipient cell and retracts to draw the two cells together. Conjugation bridge (b) An opening or pore forms between the cell walls, thereby creating a bridge to transmit genetic material. 6
(c) F Factor Transfer Bridge Donor Recipient F + F Chromosome 7
(c) F Factor Transfer Bridge Donor Recipient F + F Chromosome F + F being copied 8
(c) F Factor Transfer Bridge Donor Recipient F + F Chromosome F + F being copied F + F + (c) Transfer of the, or conjugative plasmid. A cell must have this plasmid to transfer chromosomal genes. 9
Conjugation Video 10
(d) Hfr Transfer Hfr cell Donor Recipient Integration of into chromosome Pilus Chromosome 11
(d) Hfr Transfer Hfr cell Donor Recipient Integration of into chromosome Pilus Chromosome Partial copy of donor chromosome 12
(d) Hfr Transfer Hfr cell Donor Recipient Integration of into chromosome Pilus Chromosome Partial copy of donor chromosome Bridge broken Donated genes (d) High frequency (Hfr) transfer involves transmission of chromosomal genes from a donor cell to a recipient cell. The donor chromosome is duplicated and transmitted in part to a recipient cell, where 13 it is integrated into the chromosome.
Hfr Conjugation Video 14
(a, b) Process of Conjugation (c) F Factor Transfer Bridge Donor Recipient F + F (d) Hfr Transfer Donor Recipient Pilus Hfr cell Integration of into chromosome Pilus Chromosome Chromosome F + F Partial copy of donor chromosome (a) The pilus of donor cell (top) attaches to receptor on recipient cell and retracts to draw the two cells together. being copied F + F + Bridge broken Donated genes Conjugation bridge (b) An opening or pore forms between the cell walls, thereby creating a bridge to transmit genetic material. (c) Transfer of the, or conjugative plasmid. A cell must have this plasmid to transfer chromosomal genes. (d) High frequency (Hfr) transfer involves transmission of chromosomal genes from a donor cell to a recipient cell. The donor chromosome is duplicated and transmitted in part to a recipient cell, where 15 it is integrated into the chromosome.
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transformation 17
Transformation Video 18
Generalized transduction 19
(1) (2) Phage DNA Donor (host) chromosome Cell A (donor) Parts of phage Separated piece of host DNA Phage incorporating piece of host DNA Lysis (3) DNA from donor (4) Cell B (recipient) Incorporated into chromosome (5) Cell survives and utilizes transduced DNA 20
Phage DNA Donor (host) chromosome (1) Cell A (donor) 21
Phage DNA Donor (host) chromosome (1) Cell A (donor) Parts of phage Separated piece of host DNA (2) 22
Parts of phage Separated piece of host DNA (2) Phage incorporating piece of host DNA 23
Phage incorporating piece of host DNA Lysis (3) 24
Lysis (3) DNA from donor (4) Cell B (recipient) 25
DNA from donor (4) Cell B (recipient) Incorporated into chromosome (5) Cell survives and utilizes transduced DNA 26
Transduction Video 27
Specialized transduction 28
Specialized Transduction Video 29
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