Development of Analysis Methods for Therapeutic Monoclonal Antibodies Using Innovative Superficially Porous Particle Biocolumns Anne Blackwell Bio Columns Product Support Scientist Suresh Babu Senior Application Scientist 1
Outline of Talk Introduction mabs, Characterization Workflow Solutions Reversed-Phase Challenges RP Method Development/Optimization mab Intact and Fragment Solutions Summary 2
Critical Quality Attributes and Analytical Methods RP Disulfide Shuffling SEC Aggregation Pyro- Glutamate IEX Glycosylation (G, G1, G2) HILIC Fragmentation (Hinge) RP Truncation (Lys, 1, 2) Deamidation /Oxidation IEX IEX 3
Comprehensive Portfolio of Analytical Instrumentation & Solutions for Biopharma & Biosimilars Discovery > Development > QA/QC Glycans Aggregation Molecular Weight Determination Charge Variants CE/LIF/MS, CE/MS LC/FLD, LC/MS LC/UV Size Exclusion CE/UV Field-flow fractionation CE/SDS-PAGE, CE/MS Microfluidic SDS-PAGE LC/UV or LC/MS IEF analyzer (ice 28) CE (cief), CE/MS Bio-LC/UV Ion Exchange Peptide Mapping Oxidation Amino Acids Protein PEGylation LC/UV LC/MS CE/UV, CE/MS CE/MS LC/UV LC/MS LC/UV LC/MS CE, CE/MS LC/UV SEC / Cation Exchange HIC and RP Microfluidic SDS-PAGE 4
Characterization of Monoclonal Antibodies Titer determination and purification Affinity Chromatography Protein identification and impurity profiling Reversed-phase chromatography (RP) Glycan analysis Hydrophilic interaction chromatography (HILIC) Charge variant analysis Ion exchange chromatography (IEX) Aggregation analysis Size exclusion chromatography (SEC) 5
Agilent Bio-LC Column Portfolio Multiple Affinity Removal System ZORBAX RRHD 3A 1.8um Poroshell 3 AdvanceBio RP mab ZORBAX 3SB Agilent Bio-LC Columns Affinity Reversed Phase HILIC Size Exclusion Ion Exchange Reversed-Phase Bio-Monolith AdvanceBio Columns: AdvanceBio mab Characterization Bio-Monolith Protein A AdvanceBio SEC Peptide Mapping Glycan Mapping (QA, DEAE, SO 3 ) Protein G Bio SEC-3 Bio mab ZORBAX RRHD 3-HILIC AdvanceBio RP mab Bio SEC-5 ProSEC 3S ZORBAX GF-25 Bio IEX (SAX, SCX, WAX, WCX) PL SAX PL SCX ZORBAX Amino Acid Analysis ZORBAX GF-45 PLRP-S polymeric reversed phase AdvanceBio Desalting-RP Cartridges AdvanceBio Oligonucleotide 6
Primary Structure Characterization Workflows Intact mab LC/MS Reduction / alkylation Heavy / Light Chains LC/MS, LC/UV Enzymatic digestion Fab / Fc Regions LC/MS, LC/UV Enzymatic digestion Peptides LC/UV 7
The Challenges Challenge Disadvantage Consequence Insufficient Resolution Long analysis times Short column lifetimes Method transfer to multiple columns Poor accuracy and precision for results Low number of samples run per day Must purchase new columns often Different columns for UV and MS Lack of confidence in analysis results Low throughput Poor use of resources Time consuming I M P A C T C O S T! 8
AdvanceBio RP-mAb Column with Poroshell Technology Particle 3.5 μm SP particle.25 μm porous layer depth 45Å pore diameter The optimum large molecule resolution for use with both HPLC and UHPLC systems.25 um Bonded Phases C4 3. um 3.5 um SB-C8 Diphenyl The most popular phases for proteins, plus a unique selectivity 9
Method Development: Factors Affecting the Protein Separation AdvanceBio RP-mAb Column packing (silica type and bonded phase) Column dimension Mobile phase Column temperature Linear velocity Gradient profile Sample 1
Choose The Initial Bonded Phase: C18, C8, C4 Buy Them All and Try Them All.. Not really feasible, right? How do you narrow it down? 11
Selection of Bonded Phase: Increasing protein size and hydrophobicity C18 C8 C4 Diphenyl Application Peptides and proteins, < 5 MW Hydrophobic polypeptides and proteins, Antibodies, > 5 MW Larger, hydrophobic peptides and proteins, Antibodies, > 5 MW Proteins with aromatic sidechains, Antibodies, Fusion proteins, > 5 MW RP column C18 C8 C4 Diphenyl 12
Pore size Generally speaking, the go to pore size for most protein applications using RP is > 3Å. 4 MW 4 5, MW Use smaller pore size columns to maximize loading capacity and retention Use > 3Å pore columns to maintain high efficiency. Increase column diameter to increase loading capacity 13
Mobile phase In order of increasing eluotropic force and decreasing polarity: Water, Methanol, Acetonitrile, isopropanol, THF Acetonitrile: Low UV Cutoff, 19 nm Volatile Good solubilization, denaturant Selectivity differences and sample retention will vary significantly between mobile phases ph and ionic strength of the aqueous portion of mobile phases are important in developing rugged methods It is very important to control ph to stabilize retention and selectivity % of organic solvent there is a pressure maximum and minimum for organic:aqueous mobile phases and it differs depending on the organic Use solvents that are compatible with the shipping solvent 14
Ion-pairing agent TFA Most common ion pairing agent for reversed-phase analysis Low UV absorbance Volatile Good solubilization, denaturant Acidic - improves peak shape Mild anionic ion pair - more retention of Lys, other free amines TFA / Water : TFA / ACN mobile phase Low ph (.1% TFA, ph 1.9) suppresses silanol interactions Relatively low viscosity - high efficiency, low pressure If LC/MS is used, can substitute Formic or Acetic acid 15
Column Temperature Higher column temperature can dramatically improve resolution and recovery Column temperature can be used to fine tune a separation by affecting both the retention k and selectivity Check manufacturer specs for compatibility Agilent AdvanceBio RP-mAb columns are rated to 9 o C B A 4º C 65º C A B About a 1% increase in T leads to a 1 to 2% decrease in k. Increase in T leads to decrease in pressure due to decrease in mobile phase viscosity. Increase in T also leads to decrease in peak widths. Also, use of a thermostat column compartment improves retention time precision. 5 1 Time in Minutes 16
Impact of Column Temperature on Intact mab Analysis AdvanceBio RP-mAb 1 8 6 4 2 8 C 7 C 6 C 5 C 4 C Temperature has a significant impact on run time, peak shape and recovery. Higher temperatures better with optimum at ~ 8 C for this example 1.8 1.9 2 2.1 2.2 2.3 2.4 2.5 2.6 2.7 min AdvanceBio RP-mAb SB-C4, 2.1 x 1 mm 17
Impact of Temperature on mab Fragment Analysis AdvanceBio RP-mAb Reduction Intact LC HC High resolution separation of different variant peaks at higher temperature 7 C Light chain Heavy chain 8 C 9 C AdvanceBio RP-mAb C4, 4.6 x 5 mm Agilent-RIC collaboration biopharma - Nov 214 18
Linear Velocity/Flow Rate Van Deemter Curve The smaller the plate height, the higher the plate number and the greater the chromatographic resolution 19
Linear Velocity/Flow Rate Key Chromatographic Parameters Affected by Flow Rate - Resolution - Efficiency - Peak shape - Pressure Shorten analysis time by: - Reduced column length - Increase flow rate 2
Impact of Linear Velocity on Intact mab Analysis AdvanceBio RP-mAb 16 14 12 125 1 75 5 25-25 -5-75 -1 1.7 1.8 1.9 2 2.1 2.2 2.3 2.4 2.5 min.5 ml/min.8 ml/min 1. ml/min 1.5 ml/min Linear velocity has an impact on peak shape and resolution 1 8 6 4 2 Higher velocities better with optimum at ~ 1mL/min for 2.1 mm id for this example.5 1 1.5 2 2.5 min AdvanceBio RP-mAb SB-C4, 2.1 x 1 mm 21
Impact of Linear Velocity on mab Fragment Analysis AdvanceBio RP-mAb 2 * Fab Fab Fab/2 Papain digestion 6 4 2 Fc/2 Fab/2 Fc.5 ml/min Intact Fc 6 4 2 6 4 2.8 ml/min 1. ml/min 1.8 2 2.2 2.4 2.6 2.8 3 3.2 min Linear velocity has strong impact on resolution. Higher velocities better with optimum at ~ 1 ml/min for 2.1 mm id for this example AdvanceBio RP-mAb SB-C8, 2.1 x 1 mm 22
Gradient Slope Gradient steepness effects retention (k*) and resolution Adjust gradient slope to optimize resolution - accomplished by changing: - gradient time t G (most common way to change gradient steepness) - % change in organic modifier over time 23
Impact of Gradient Profile on mab Fragment Analysis AdvanceBio RP-mAb 2 * Fab 8 5 to 3% 5 to 4% 5 to 5% 5 to 6% Intact Papain digestion Fc 6 4 Gradient profile has impact on selectivity and resolution 2 Steeper gradient better with optimum 5 5% for this example 1.5 2 2.5 3 3.5 4 min AdvanceBio RP-mAb SB-C8, 2.1 x 1 mm 24
Column Robustness High Reproducibility Low Carry-over Long Column Lifetime 25
Reproducibility AdvanceBio RP-mAb Injection to injection repeatability supports consistent column performance Intact Reduced B A C Analysis of intact mab Analysis of reduced mab A B Average %RSD Average %RSD RT 4.544.9 RT 3.234.6 A Area 4521.294.9 Area 496.162.91 RT 4.8494.5 RT 4.6574.4 B Area 158.43.8 Area 99.85.69 RT 5.118.2 C Area 127.28 1.1 AdvanceBio RP-mAb SB-C4, 4.6 x 5 mm 26
No Carry-Over AdvanceBio RP-mAb No protein carry-over between runs Intact Injection of 3 production batches of intact mab each followed by 1 blank injection, shows no carry-over Zoom view Reduced Injection of 3 production batches of reduced mab each followed by 1 blank injection shows no carry-over Zoom view AdvanceBio RP-mAb SB-C4, 4.6 x 5 mm 27
Long Column Lifetime AdvanceBio RP-mAb AdvanceBio RP-mAb SB-C8 Stable over 1, injections = 4, column volumes Peak Compond RT_1st Injection RT_13 Injection RT change (min) RT change% 1 Ribonuclease A.957.942.15 1.57% 2 Lysozyme 1.872 1.86.12.64% 3 Cytochrome C 2.111 2.97.14.66% 4 α-lactalbumin 2.99 2.899.1.34% 5 Catalase 6 Carbonic Anhydrase 3.994 3.981.13.33% Retention time [min] 4 3 2 1 2 4 6 8 1 Ribonuclease A Cytochrome C Lysozyme α-lactalbumin Carbonic Anhydrase AdvanceBio RP-mAb SB-C8 # of injections 28
Excellent Batch-to-Batch Reproducibility AdvanceBio RP-mAb (different column batches) Reproducible retention time and peak shapes over 6 different column batches Batch 1 1 2 3 4 5 6 Batch 2 Batch 3 Batch 4 Samples 1. Ribonuclease A 13.7 kda 2. Cytochrome C 12 kda 3. Holo-Transferrin 8 kda 4. α-lactalbumin 14.2 kda 5. Catalase 24 kda 6. Carbonic Anhydrase 29 kda Batch 5 Batch 6 AdvanceBio RP-mAb SB-C4, 2.1 x 1 mm 29
Fast Intact mab Analysis AdvanceBio RP-mAb AdvanceBio RP-mAb C4 provides a sharp peak and resolves fine detail in less than two minutes AdvanceBio RP-mAb Diphenyl resolves additional fine detail - the Diphenyl phase is unique to Agilent 14 12 1 8 6 AdvanceBio RP-mAb C4 AdvanceBio RP-mAb SB-C8 AdvanceBio RP-mAb Diphenyl 14 12 1 8 6 4 2-2 -4 4 1.7 1.8 1.9 2 2.1 2.2 2.3 2.4 2.5 min 2 1.7 1.8 1.9 2 2.1 2.2 2.3 2.4 2.5 min All phases provides good separation of intact mab with AdvanceBio RP-mAb Diphenyl resolves additional fine detail. 3
Fast Intact mab Analysis AdvanceBio RP-mAb AdvanceBio RP-mAb C4 provides better resolution than protein columns from competitors Zoom view 1 1 AdvanceBio RP-mAb C4, 45Å, 3.5 μm 5 5-5 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 min 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 min 1 Brand A 5 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 min 1 C4, 4Å, 3.4 μm 5-5 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 min 1 5 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 1 Brand B Brand C 5 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 1 C4, 2Å, 3.6 μm 5-5 min 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 1 C4-3, 3Å, 2.6 μm 5-5 min 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 min min 31
High Resolution Separation of Intact mab AdvanceBio RP-mAb AdvanceBio RP-mAb C4 provides superior peak shape at a lower pressure than a UHPLC protein column from a competitor 1 AdvanceBio RP-mAb C4, 45Å, 3.5 μm 49 bar 5 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 min Brand D C4, 3Å, 1.7 μm 1 5 91 bar 1.3 1.4 1.5 1.6 1.7 1.8 1.9 2 2.1 2.2 min 32
Fast & High Efficiency Separation of Intact mab AdvanceBio RP-mAb AdvanceBio RP-mAb provides superior peak shape x1 1 3.6 3.2 2.8 Width =.219 Width =.187 Width =.227 2.4 2 1.6 AdvanceBio RP-mAb C4, 45Å, 3.5 μm AdvanceBio RP-mAb Diphenyl, 45Å, 3.5 μm Brand A, C4, 3Å, 3.5 μm 1.2.8.4.5 1 1.5 2 2.5 3 3.5 4 4.5 5 min 33
High Resolution mab Fragments: LC/UV AdvanceBio RP-mAb AdvanceBio RP-mAb provides superior peak shape and high resolution than competitor protein column Reduced mab Reduction AdvanceBio RP-mAb C4, 45Å, 3.5 μm Brand A, C4, 3Å, 3.5 μm Intact LC HC m AU 6 5 4 3 2 1 LC HC Rs = 2. Rs =.6 m AU 8 6 4 2 4 3 2 1 LC LC HC HC Rs = 3.4 Rs =.8 1 2 3 4 5 6 7 8 9 m i n mab1 1 2 3 4 5 6 7 8 9 m i n mab2 34
High Resolution ADC Fragments: LC/UV AdvanceBio RP-mAb Separation of different drug conjugated species ADC: Lys conjugated Reduced ADC Papain digested ADC 3 2.5 2 1.5 1.5 3 2.5 2 1.5 1.5 L L L1, L2 AdvanceBio RP-mAb C4, 45Å, 3.5 μm H, H1, H2, H3 L1, L2 H, H1, H2, H3 6 Brand A, C4, 3Å, 3.5 μm 5 4 3 2 1 7 6 5 4 3 2 1 Fc - 1 Fc 2 * Fab - 2 2 * Fab 1 1.5 2 2.5 3 3.5 4 4.5 5 5.5 6 6.5 7 7.5 8 8.5 9 min 1 2 3 4 5 6 7 8 9 1 min 35
High Resolution ADC Fragments: LC/UV AdvanceBio RP-mAb Separation of different drug conjugated species Reduced ADC Light chain Heavy chain Lys-conjugated ADC Light chain Heavy chain Cys-conjugated ADC AdvanceBio RP-mAb Diphenyl 36
High Resolution mab Fragments: LC/UV AdvanceBio RP-mAb Separation of stressed-induced antibody variants Stressed variants Herceptin intact ph stressed Herceptin Oxidized Herceptin mab ph stressed mab Oxidized mab reduced Herceptin ph stressed Herceptin Oxidized Herceptin Light chain Heavy chain AdvanceBio RP-mAb C4, 4.6 x 5 mm 37
Fast, High Resolution mab Fragment Analysis AdvanceBio RP-mAb AdvanceBio RP-mAb provides superior peak shape and resolution than other columns designed for protein separations 2 Fc/2 Fab/2 Fc Fab/2 Fab Intact Papain digestion AdvanceBio RP-mAb C4, 45Å, 3.5 μm 2 * Fab Fc 2 2.25 2.5 2.75 3 3.25 3.5 3.75 4 4.25 min 2 Brand A C4, 4Å, 3.4 μm 2 2.25 2.5 2.75 3 3.25 3.5 3.75 4 4.25 min 2 Brand B C4, 2Å, 3.6 μm 2 2.25 2.5 2.75 3 3.25 3.5 3.75 4 4.25 min 2 Brand C C4-3, 3Å, 2.6 μm 2 2.25 2.5 2.75 3 3.25 3.5 3.75 4 4.25 min 38
Fast, High Resolution mab Fragment Analysis AdvanceBio RP-mAb Separation of mab subunits with superior peak shape and resolution Ides digestion 2*LC 2 * Fd 2.5 Fd Intact Reduction 2*Fc 2 LC 1.5 Fc/2 1.5 1 2 3 4 5 6 7 8 9 1 11 12 13 14 min AdvanceBio RP-mAb SB-C8, 2.1 x 5 mm 39
Benefits of AdvanceBio RP-mAb Columns Pain Features and Advantages Benefit Insufficient resolution Long analysis times Short column lifetime Superficially porous particles of smaller particles (3.5 µm) with wide pore (45Å) increase resolution for mab but maintain compatibility with all LC instruments Due to Poroshell technology, analysis time of mab characterization has been shown to decrease significantly over fully porous particles of the same size Column with robust Poroshell packed bed and with 2 µm frit decreases chances of bedcollapse or inlet blockage Improved confidence in analysis results (accuracy) Improved throughput - reduced costs Improved resource use - reduced costs Agilent Confidential 4
Summary AdvanceBio RP-mAb AdvanceBio RP-mAb columns - analyzing monoclonal antibodies for biopharma discovery, development, and QA/QC applications Improved accuracy: Superficially porous particles (3.5 μm) with wide pores (45Å) increase mab resolution while maintaining compatibility with all LC instruments Speed: Shorter analysis times compared to columns packed with fully porous particles of the same size Lower costs: The robust Poroshell packed bed and 2 μm inlet frit extend column lifetime by helping prevent inlet blockage Flexible method development: Range of chemistries SB-C8, C4, and Diphenyl 41
Q & A 42