AND HAZARD TO TUBER HEALTH

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POTATO PEST SYMPTOM SHEETS AND HAZARD TO TUBER HEALTH KAPCHORWA SEED POTATO PRODUCERS ASSOCIATION WHAT IS THE PURPOSE OF THE SEED PEST HAZARD SHEETS? The Seed Pest Hazard Sheets associate plant symptoms with possible pests, they do not necessarily give an absolute identification on a particular pest Based on the recognition of the pests associated with a Pest Symptom Class, a hazard status is established that relates specifically to the suitability of tubers of those plants affected by a symptom class for use as seed In most cases an appropriate crop management intervention step can then be implement to limit exposure of neighbouring plants to the hazard The Seed Pest Hazard Sheets, when used with the KASPPA Crop History Sheet and Pest Risk Analysis Sheet, provide a robust pathway for quality assured, identity preserved production of potato tubers suitable for use as seed WHO ARE THE SEED PEST HAZARD SHEETS FOR? The Seed Pest Hazard Sheets are for farmers and field inspectors interested in potato production suitability for use as seed OTHER MATERIALS IN SUPPORT OF THE SEED PEST HAZARD SHEETS The Seed Pest Hazard Sheets need to be worked with the KASPPA Crop History Sheet and Pest Risk Analysis Sheet The Crop History Sheet is a written farmer records on the management of their potato crop over a season and the pest problems observed The Pest Risk Analysis Sheet looks to combine information from the Seed Pest Hazard Sheet and the Crop History Sheet in arriving at an evaluation on the health of a crop, particularly in respect of pests that may be tuber-borne and affecting the suitability of those tubers for use as seed

POTATO PEST SYMPTOM CLASS PRIMARY SYMPTOM CLASS: GREEN WILT Wilt occurs rapidly and the plant remains green; the plant does not recover overnight Typically not the entire plant is affected at the early stage, with symptoms centred on 1 or 2 stems Affected plants may be scattered or clustered about the field For Bacterial Wilt On pulling the plants the stems does not break easily Whereas for insect / hoe damage On pulling the plant the stem breaks easily at the root surface, but is not wet with rot And for Black Leg The stem may break easily and is wet with rot SECONDARY SYMPTOM CLASSES INSECT / HOE DAMAGE BLACK LEG BACTERIAL WILT LOW HAZARD MODERATE HAZARD HIGH HAZARD

POTATO PEST SYMPTOM CLASS PRIMARY SYMPTOM CLASS: YELLOW WILT Wilt occurs slowly and the plant goes yellow Typically part or the entire plant is affected at the early stages On pulling the plants the stems does not break easily Affected plants tend to be clustered in one region of the field, forming a patch, though scattering may also be noticed For Verticillium Wilt The leaves will show yellowing on half of the leaf only Whereas, for Fusarium Wilt and Root Knot Nematode The leaf will show an all over yellowing SECONDARY SYMPTOM CLASSES The above symptom is common to all wilts [Fusarium, Verticillium and Bacterial wilt] VERTICILLIUM WILT FUSARIUM WILT ROOT KNOT NEMATODES MODERATE HAZARD MODERATE HAZARD MODERATE HAZARD

POTATO PEST SYMPTOM CLASS PRIMARY SYMPTOM CLASS: LEAF SPOTS AND FOLIA DAMAGE For Late Blight Leaf spots spread rapidly and with uneven shape Weather is wet and humid Affected plants tend to be clustered in one region of the field Whereas for Early Blight Leaf spots spread slowly and form rings The symptom may be more in one region of the field And for insects Parts of the leaves are removed, leaving a hole or a transparent area SECONDARY SYMPTOM CLASSES LATE POTATO BLIGHT EARLY BLIGHT INSECT HIGH HAZARD LOW HAZARD LOW HAZARD

POTATO PEST SYMPTOM CLASS PRIMARY SYMPTOM CLASS: LEAF MOSAICS AND MISS-SHAPEN PLANT PARTS For viruses Symptoms may be diverse, including Stunted growth Chlorotic or purple tinged leaves Rolled leaves Multi-stemmed with mini tubers Affected plants are likely to be scattered across the field, rather than clustered And for varietal off-types Scattered plants have distinct flower and / or foliage characteristic Whereas for chemical damage Large areas of the field will be equally affected New leaf growth may be normal Image needed Plants are healthy, but noticeable for having atypical foliage or flower characteristics VIRUSES MODERATE HAZARD VARIETAL OFF-TYPES MODERATE HAZARD CHEMICAL DAMAGE LOW HAZARD

POTATO PEST SYMPTOM CLASS PRIMARY SYMPTOM CLASSES IN STORAGE This symptom of a cavity with white fluff is unlikely to be seen in the field, but may be common amongst stored tubers DRIED TUBER: FUSARIUM WILT MODERATE HAZARD Mud sticks to the eyes of the tubers that may ooze a white liquid On cutting the tuber a ring is seen that is wet and, when pressed, oozes a white liquid TUBER WITH WHITE OOZE: BACTERIAL WILT HIGH HAZARD Potato is soft and wet On cutting the tuber brown regions of soft rot are seen No ring is seen [as with Bacterial Wilt] WET TUBERS: LATE BLIGHT AND SOFT ROTS [BLACK LEG] HIGH HAZARD Tuber surface has black speckles [black scurf] that can be picked off Tuber surface is pitted and corky SURFACE SCABS: BLACK SCURF [LEFT] & SCAB [RIGHT] Images needed MODERATE HAZARD Both larval and adult stages may be observed TUBER DAMAGE / TUNNELLING: TUBER MOTH MODERATE HAZARD This publication is an output from a research project funded by the United Kingdom Department for International Development [DFID] for the benefit of developing countries. The views expressed are not necessarily those of DFID [Crop Protection Programme]

KAPCHORWA SEED POTATO PRODUCERS ASSOCIATION PEST RISK ANALYSIS SHEET FOR SEED FIELD PEST SYMPTOM CLASS Primary Symptom Classes Secondary Symptom Classes Probable pest identification Gap None Numerous causes Green wilt Yellow wilt Leaf spots and folia damage Stem / root not damaged Stem / root damaged, no rot Stem / root damaged, wet rot Plant roots normal Plant roots miss-shapen Leaf spots large and variously shaped Leaf spots small and circular Parts of leaves missing or transparent Symptoms scattered Bacterial Wilt Insect / hoe damage Black Leg Fusarium Wilt or Verticillium Wilt Root Knot Nematode Late Blight Early Blight Insect damage Leaf mosaics Virus and missshapen plant Atypical healthy plants Varietal off-types parts Large area affected Chemical damage Hazard High Observation [Running total of incidence] Records at emergence Records at early flowering Records at late flowering Number of tubers planted % incidence Hazard rating High 5 Low 0 High 5 Low 0 Low 0 Low 0 RISK VALUE TOTAL Risk Value [mean % infection x hazard rating] KASPPA Code Number:

KAPCHORWA SEED POTATO PRODUCERS ASSOCIATION PEST RISK ANALYSIS SHEET FOR SEED STORAGE PEST SYMPTOM CLASS Primary Symptom Classes Dried tuber Soil and white ooze in tuber eyes Tuber looses shape and is wet Scabs on tuber surface Tuber damage / tunnelling Secondary Symptom Classes Dried hole seen inside tuber with white fluff On cutting tuber, ring oozes a white liquid On cutting, brown wet rot is seen [no ring] Scabs are black and are picked of easily Scabs are sunken and corky Larva and moths present Probable pest identification Fusarium Wilt Bacterial Wilt Late Blight / Soft Rot Black Scurf Scab Tuber moth Hazard Observations on number of affected tubers per 100 tubers sampled in storage Sample 1 Sample 2 Sample 3 % incidence [mean of samples] Hazard rating High 5 High 5 Risk Value [% incidence x hazard rating] RISK VALUE TOTAL Primary Symptom Classes Soil and white ooze in tuber eyes Tuber looses shape and is wet INCUBATION TEST Secondary Symptom Classes On cutting tuber, ring oozes a white liquid On cutting, brown wet rot is seen [no ring] Probable pest identification Bacterial Wilt Late Blight / Soft Rot Hazard High Observations on number of affected tubers per 100 tubers sampled under incubation Sample 1 Sample 2 Sample 3 % incidence [mean of samples] Hazard rating 5 Risk Value [% incidence x hazard rating] RISK VALUE TOTAL KASPPA Code Number: This publication is an output from a research project funded by the United Kingdom Department for International Development [DFID] for the benefit of developing countries. The views expressed are not necessarily those of DFID [Crop Protection Programme]

CROP HISTORY SHEETS KAPCHORWA SEED POTATO PRODUCERS ASSOCIATION KASPPA RESERVES THE RIGHT TO REJECT A FARMERS HARVEST WHERE THE CROP HISTORY SHEETS ARE NOT COMPLETED TO THEIR SATISFACTION It is the responsibility of the farmer to record the information on the potato crop as requested by the Crop History Sheets in the field and in-storage WHO ARE THE CROP HISTORY SHEETS FOR? The Crop History Sheet is for farmers wishing to produce potato to be sold through KASPPA as material suitable for seed KASPPA field inspectors will review the Crop History Sheet in their assessments on the health status of the crop WHAT DOES THE CROP HISTORY SHEETS DO? Provides a written record of the farmers field and in-storage management practices for review by KASPPA field inspection staff Importantly it looks to map the Pest Symptom Classes observed within the crop and show how the farmer implemented an activity to reduce the hazard posed by that pest to neighbouring plants and tubers By the correct use of the Crop History Sheet a farmer will be able to demonstrate to KASPPA that good management practices were adhered to HOW WILL THIS INFORMATION BE USED? Information on the Crop History Sheet will be reviewed by KASPPA field inspectors The KASPPA field inspectors will compare the information on the Crop History Sheet with the observations made during their inspection of the crop From these activities the KASPPA inspectors will arrive at a recommendation to accept or reject the crop as suitable for sale through KASPPA as seed This publication is an output from a research project funded by the United Kingdom Department for International Development [DFID] for the benefit of developing countries. The views expressed are not necessarily those of DFID [Crop Protection Programme]

KASPPA CROP HISTORY SHEETS KASPPA Code No: Farmer name & address No of seed bags Where from: Kg: Seed lot No. [e.g. KASPPA; UNSPPA]: received Area planted [ha] Date of tuber receipt Sprouting condition Field history Last season: Season before last: Field 1 choice and Describe: Date of planting: preparation Fertilizer application Date: Composition: Quantity: Date: Composition: Quantity: Fungicide application Date: Composition: Quantity: Date: Composition: Quantity: Date: Composition: Quantity: Date: Composition: Quantity: Insecticide Date: Composition: Quantity: application Date: Composition: Quantity: Weeding First date: Second date: Third date: Earthing-up First date: Second date Field pest symptom observations Draw a picture of your crop and map all observations on potential pests [Symptom Classes] as described in the Seed Hazard Symptom Sheets [see attached sheets for details]. Note the definition of a field as given at the bottom of this sheet Date: Dehaulming Harvest Date: Expected yield in 80kg bags: Harvest yield Category 1 [Mini-seed; <30mm] Bags: Kg: Date put into storage In-storage pest symptom observations Management of next seasons land Category 2 [Size 1 Seed; 30 45mm]] Bags: Kg: Category 3 [Size 2 Seed; 45 60mm] Bags: Kg: Category 4 [Table; >60mm] Bags: Kg: Draw a picture of your store and map all observations on potential pests [Symptom Classes] as described in the Seed Hazard Symptom Sheets [see attached sheets for details]. Note that each storage shelf will need its own map 2004a 2004b Location 1 A field is a defined as a continuous area of land planted to the same source of seed. Separate areas of land planted with the same seed source and continuous areas of land planted with different sources of seed need separate Crop History Sheets

KASPPA CROP HISTORY SHEETS CROP HISTORY FIELD MAP SHEETS FOR FIELD AND STORAGE WHY IT IS IMPORTANT TO MAP PEST PROBLEMS? The undertaking of the maps in conjunction with the Crop History Sheets will demonstrate that the farmer has been vigilant in observing pest problems and has undertaking effective management practices to reduce the risk of spread to uninfected plants Pests are often distributed unevenly across a field or in-storage. By identifying those areas with a high pest problem it may be possible to separate out that portion of the production that can still go forward to KASPPA as suitable for planting HOW TO USE THE KASPPA CROP HISTORY FIELD MAP SHEET Imagine that you are directly above your field, like a bird looking down On the KASPPA Crop History Field Map Sheet draw what you see, the map of your crop, record the number of rows, their length, see what is bordering your potato crop Now, come down and walk through your crop using your map as a guide Using the Seed Pest Hazard Sheets, observe your field for Pest Symptom Classes and potential pests Place a stake by these plants. Then note on your map where these affected plants are, the type of Pest Symptom Class [See Pest Symptom Class Code Sheet] and date your observation Based on the recognition of a Pest Symptom Class, implement an appropriate action to reduce exposure of neighbouring plants to any hazard, and record this action HOW TO USE THE KASPPA CROP HISTORY STORAGE MAP SHEET The Crop History Storage Map Sheet follows the same principles as set out for the KASPPA Crop History Field Map Sheet It asks that you look down on your stored potato and map how it is set out. But, here you need to recognise that you may have more than one layer of potato. In these cases use separate Crop History Storage Map Sheets for each layer of seed in storage Now observe the stored potato for Pest Symptom Classes as shown in the Seed Pest Hazard Sheets and record any observations using the appropriate codes as described on the Pest Symptom Class Code Sheet, noting the position of the Pest Symptom Class and the date Implement an appropriate action to reduce exposure of neighbouring tubers to any hazard presented by the Pest Symptom Class, and record this action

KASPPA CROP HISTORY SHEETS PEST SYMPTOM CLASS CODE SHEET Probable pest FIELD PEST SYMPTOM CLASS identification Primary Symptom Secondary Symptom Classes Classes Gap None Numerous causes Green wilt Yellow wilt Leaf spots and folia damage Leaf mosaics and missshapen plant parts Stem / root not damaged Stem / root damaged, no rot Stem / root damaged, wet rot Plant roots normal Plant roots miss-shapen Leaf spots large and variously shaped Leaf spots small and circular Parts of leaves missing or transparent Symptoms scattered Atypical healthy plants Large area affected Bacterial Wilt Insect damage Black Leg Fusarium Wilt or Verticillium Wilt Root Knot Nematode Late Blight Early Blight Insect damage Virus Off-types Chemical damage Pest Symptom Class Code [To be used with Crop History Sheets] G GW-BW GW-In GW-BL YW-W YW-N L-LB L-EB L-In L-V L-OT L-Ch STORAGE PEST SYMPTOM CLASS Primary Symptom Secondary Symptom Classes Classes Dried tuber Dried hole seen inside tuber with white fluff Soil and white ooze in On cutting tuber, ring oozes a tuber eyes white liquid Tuber looses shape and is On cutting, brown wet rot is seen wet [no ring] Scabs on tuber surface Scabs are black and are picked of easily Scabs are sunken and corky Tuber damage / tunnelling Larva and moths present Probable pest identification Fusarium Wilt Bacterial Wilt Late Blight / Soft Rot Black Scurf Scab Tuber moth Pest Symptom Class Code [To be used with Crop History Sheets] FW BW SR Sc-BS Sc-S TM

KASPPA CROP HISTORY FIELD MAP SHEET KASPPA Code No:

KASPPA CROP HISTORY STORAGE MAP SHEET KASPPA Code No:

KASPPA CROP HISTORY SHEETS KASPPA Code No: KASPPA COMMENTS AT EMERGENCE: DATE AT EARLY FLOWERING: DATE AT LATE FLOWERING: DATE DURING STORAGE: DATE Declaration: It is agreed between the farmer and KASPPA that the attached Crop History Sheets represent a fair summary of the management practices implemented and Pest Symptom Classes observed for KASPPA production Code Number. Name of KASSPA Inspector: Name of Farmer: Signature of KASPPA Inspector: Signature of Farmer:

POTATO SEED PLOT SYSTEM TRAINING GUIDE KAPCHORWA SEED POTATO PRODUCERS ASSOCIATION THE IMPORTANCE OF USING GOOD QUALITY SEED A key aspect of potato production is the planting of good seed. However, good potato seed is scarce and expensive. These factors make the practice of farmers saving their own seed over seasons common. An increase in pests is favoured by this approach. The Seed Plot System [SPS] described below presents a way for farmers to access affordable, small quantities of good quality seed from KASPPA for multiplication. This seed is then used for Table production in the next growing season. This presents a way to replenish old seed with higher yielding seed of familiar or new varieties. WHEN TO USE THE SEED PLOT SYSTEM [SPS] AND WHY When land for planting is limited and a good rotation with non potato-like [nonsolanaceous] crops is not possible When availability of good seed is limited When your potato seed has been saved for many seasons When you would like to try out a new variety When you are looking to start potato production To optimise land use To optimise use of quality assured KASPPA seed To reduce seed borne pests To optimise production of seed To optimise yield and quality of Table potato The method of seed production was developed by farmers in Kenya and Uganda WHERE TO HAVE YOUR SPS Bacterial wilt and many other pest problems are made worse by planting unhealthy seed on poor soil. Here, a wilted plant and tuber with ooze; symptoms typical of bacterial wilt, a serious disease of potato The potato seed should be planted on land that was not to potato or another potato-like [solanaceous] crop during the previous 2 seasons The neighbouring cultivations should also be clean of potato and solanaceous crops Look to avoid planting on land with weed [volunteer] potato Look to avoid run-off water from other potato cultivations Locate the SPS close to your home so you can manage it closely, provided the land is clean of pests

THE TYPICAL SEED PLOT SYSTEM The width of the plot is not less than 1.5m [one person in height] The length of the plot is defined by the number of seed The land is well dug [double dug] and a flat seed bed is prepared. The tubers are planted close together [20 cm x 20cm, or 30 x 30cm]. This is to encourage the production of tubers of a seed size Planting is by making a furrow that is planted and then covered when preparing the next furrow Only simple tools are needed, such as a jebbei. Earthing-up promotes good tuber production and should be done by taking soil from the sides of the plot Focus resources on pest control; do not use fertilizer unless your soil is very poor Farmers in Kabale discuss the SPS with extension staff. Notice the thin width of the plot and the height achieved through earthing-up from the edges WHAT SEED AND HOW TO MANAGE THE SPS Purchase KASPPA seed; this seed is of an assured quality. Look for the seed size class [<30mm]. Do not mix with other farm seed. Farmers seed carries the risk of pests that will spoil your harvest Store in a well ventilated, dry, half-light environment to encourage strong sprouting The tubers should be well sprouted prior to planting Successful Table production in Kapchorwa from planting healthy good quality seed No fixed method describes the management of the SPS The SPS provides a window for intensive seed production Be aware for early signs of pests and implement control as advised by KASSPA or Extension staff The SPS can be harvested 2 to 3 weeks earlier than the Table field production. Dehaulm at flower drop and harvest 2 weeks afterwards Early harvesting allows more time for good sprouting for next seasons planting Small seed, not suitable for Table production, can reliably be made use of by the SPS, making best use of your seed This publication is an output from a research project funded by the United Kingdom Department for International Development [DFID] for the benefit of developing countries. The views expressed are not necessarily those of DFID [Crop Protection Programme]