Innovative Natural Products for Agricultural Pest and Water Resource Management

Innovative Natural Products for Agricultural Pest and Water Resource Management

Ground Breaking Ceremony at Bangor, Michigan New Manufacturing Facility August 2012

Broad Spectrum Bioinsecticide

Grandevo Insecticide/Miticide Proven Broad Spectrum Control Chewing and sucking insects Mites Naturally Derived From Chromobacterium subtsugae Complex Mode of Action Easy on Beneficials 4 Photo Credits:: Lygus Entomart; Beet armyworm Clemson Univ. USDA Cooperative Extension Slide Series Bugwood.org; Western flower thrip Frank Peairs, CO St. Univ. Bugwood.org; citrus leaf miner Center for Invasive Species Research, UC Riverside; : cabbage Looper RJ Reynolds Tobacco Company, Bugwood.org; twospotted spider mite Clemson EDU

Selective, Broad Spectrum Protection Insecticidal & miticidal properties Complex mode of action Bacteria produces multiple compounds Activity stems from the combination of the compounds Activity includes repellency, reduced fecundity, and death via ingestion Performance comparable to mainstream chemical insecticides Doesn t interfere with beneficial insect activity 5 Photos courtesy of: Cabbage Looper RJ Reynolds Tobacco Company, Bugwoodorg; aphid Whitney Cranshaw, CO State University, Bugwood.org; ; citrus leaf miner Center for Invasive Species Research, UC Riverside; pepperweevil Alton N. Sparks, Univ. of GA Boxwood.org; twospotted spider mite Clemson EDU

Grandevo A New Tool for Modern Pest Management Broad spectrum protection against sucking and chewing species Complex mode of action Easy on beneficials REI=4 hours / PHI=0 days Tolerance exempt NOP compliant and OMRI approved Field or greenhouse applications Ground or aerial applications 6

Effect of Grandevo on Potato Psyllid Oviposition Mean no. of eggs laid/4 females 140 120 100 80 60 40 20 0 Negative Ctrl Grandevo Std 3 4 5 6 7 10 Days after Treatment

Grandevo vs. Spotted Wing Drosophila Spotted Wing Drosophila (Drosophila suzukii) on Strawberry - Cage Study San Luis Obispo, CA, 2012 a # of Larvae / Fruit a b a a b b b 1lb Silwet L-77 0.05% v/v Untreated Control 8 - Application Dates: Jan 30 Feb 6 13 15 22 29 - Grandevo: Chromobacterium subtsugae

Grandevo vs. Twospotted Spider Mite Twospotted Spider Mite on Strawberry (Tetranychus urticae) Univ. of Florida, Gulf Coast Research Center Wimauma, FL, 2012 Number of Motiles / leaflet 9 1.0 lb Induce 0.25% v/v Oberon 2SC 12 fl.oz. 2.0 lb Induce 0.25% v/v Agri-Mek 0.15EC 16 fl.oz. CoHere 16fl.oz. Savey 50DF 6 oz Applied once - Jan 6 Application Dates: Jan 10 19 25 Feb 2 10 16 Evaluation Dates: Jan 13 17 Grandevo: Chromobacterium subtsugae Untreated Control

VENERATE TM New Microbial Insecticide (MBI 206) Confidential Isolated from MBI s international screening program New bacterial species of Burkholderia with no established relationship to opportunistic Burkholderia species. One patent granted (Sept 2011) and another filed (Aug 2012) regarding strain and chemistry Tox and ecotox show no issues to all tested non target organisms

VENERATE TM New Microbial Insecticide (MBI 206) Microbial extract (non viable) submission Oct 2010 to EPA/PMRA. Expect registration early 2013 Confidential Broad spectrum sucking and chewing insects and flies Kills via Contact and Feeding One active insecticidal chemical structure identified. Further characterization of active structures ongoing.

VENERATE TM Biological Activity Summary CONFIDENTIAL Laboratory, greenhouse and field studies confirm activity against economically significant pests

Biologically active against economically important insect pests in laboratory studies CONFIDENTIAL Chewing insects Beet armyworm Cabbage looper Tobacco budworm Diamondback moth Codling moth Tufted apple budmoth Obliquebanded leafroller Sucking insects Pea aphid Green peach aphid Western flower thrips Silverleaf whitefly Twospotted spider mite Asian citrus psyllid Potato psyllid Brown marmorated stink bug Sponging insects Housefly

CONFIDENTIAL Several Key Field Efficacy Studies

3.5 3 Control of Western Flower Thrips, Frankliniella occidentalis, on Strawberry in California # of Thrips / 10 leaves CONFIDENTIAL 2.5 2 1.5 1 UTC MBI 206 @ 2 gal Movento @ 6 fl oz 0.5 0 18 Oct 27 Oct 1 Nov 8 Nov 15 Nov Four applications included Silwet L-77 at 0.25% v/v

60 50 Control of Western Flower Thrips, Frankliniella occidentalis, on Peppers in Mexico Thrips / 10 Flowers a a a CONFIDENTIAL 40 30 20 b b b bc bc 10 c c c c c c c c c c 0 MBI-206 EP 5 l/ha MBI-206 EP 10 l/ha MBI-206 EP 15 l/ha MBI-206 EP 20 l/ha Tracer 100 ml/ha Untreated Control - Application Dates: June 9 16 - Thrips counts: June 9 16 23 30 - Statistical comparison only valid between same count dates.

Control of pepper weevil, Anthomonus eugenii, in Florida Dr. D. Seal U. of FL 18 16 Mean # of weevil infested, fallen fruits per plot a CONFIDENTIAL 14 12 10 8 abc bcde 6 4 2 de e cde 0 MBI 206 @ 2 qt MBI 206 @ 4 qt MBI 206 @ 6 qt Assail 30SC @ 4 oz Actara @ 5 oz Untreated Applications done: Dec. 14, 21, 28 and Jan. 5

Control of Asian Citrus Psyllid on Citrus Dr. Phil Stansly, U. of FL 12 10 # nymphs/shoot CONFIDENTIAL 8 6 4 3 DAT 10 DAT 17 DAT 24 DAT 2 0 Untreated MBI 206 @ 2 gal Danitol+oil Agri flex+oil Application volume @ 120 GPA; Treatments: MBI 206 EP @ 2 gal + NIS; Danitol 2.4EC @ 16 oz + 2% 435 oil; Agri Flex @ 8.5 ounces + 2% 435 oil.

Control of Asian Citrus Psyllid on Citrus Dr. Phil Stansly, U. of FL 0.7 0.6 # adults/tap CONFIDENTIAL 0.5 0.4 0.3 0.2 3 DAT 10 DAT 17 DAT 24 DAT 0.1 0 Untreated MBI 206 @ 2 gal Danitol+oil Agri flex+oil Treatments @ 120 GPA; MBI 206 EP @ 2 gal + NIS; Danitol 2.4EC @ 16 oz + 2% 435 oil; Agri Flex @ 8.5 ounces + 2% 435 oil.

CONFIDENTIAL Steven K. Whitesides, Ph.D. Western Regional Technical Service & Product Development Manager swhitesides@marronebio.com