National Plant Diagnostic Network

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National Plant Diagnostic Network Kitty Cardwell, PhD National Program Leader USDA Cooperative State Research Education & Extension Service 1 st NPDN National Meeting A RECORD OF ACCOMPLISHMENT Orlando, Florida January, 28 2007

Are our plant systems vulnerable? New York, 9.11.01 For the life of me, I can t understand why the terrorists haven t attacked our food supply, because its so easy to do. Health and Human Services Secretary Tommy Thompson

Patriot Act October 2001 The uniting and strengthening of America by providing appropriate tools required to intercept and obstruct terrorism. Makes it illegal for anyone to possess any biological agent for any inappropriate reason. Prohibits the transfer or possession of a listed biological agent or toxin by a restricted person.

Bioterrorism Response Act June 2002 Adds new requirements for the secretaries of the Departments of Agriculture and Health and Human Services to consider in listing agents and in preventing unlawful access to agents during transfers.

Homeland Security Presidential Directive - 9 develop robust comprehensive, and fully coordinated surveillance and monitoring systems, for plant diseases provides early detection and awareness of disease, pest or poison establishes nationwide laboratory networks for food, veterinary, plant health, and water quality that integrate existing Federal and State laboratory resources, are interconnected, and utilize standardized diagnostic protocols and procedures

COOPERATIVE AGREEMENTS between University of and CSREES, USDA Situation In determining the best use of the funds Congress appropriated in the Fiscal Year 2002 Emergency Supplemental for Recovery from and Response to Terrorist Attacks on the United States, USDA assessed the greatest risks to American Agriculture. We focused particularly on those threat agents, animal and plant pests and diseases, which terrorists or others could intentionally introduce into our country or spread from isolated parts of the country. Purpose USDA plans to establish a unified network of public agricultural institutions to identify and respond to high risk biological pathogens in the food and agricultural system. The core of the network will be 5 animal and 5 plant Core diagnostic laboratories plus 6 Satellite animal diagnostic laboratories dispersed strategically around the country. They will develop a two-way, secure communications network with other university and State Department of Agriculture diagnostic laboratories throughout their respective regions. These Core diagnostic laboratories will be responsible for deploying standardized diagnostic approaches for identification of exotic and domestic pests and pathogens that are of biosecurity concern. A plant and animal disease diagnostic database will include test procedures, experts, and identification data. These efforts will supplement other initiatives and provide Animal and Plant Health Inspection Service (APHIS) and the Agricultural Research Service (ARS) back-up and independent confirmation of diagnostic results.

National Plant Diagnostics Network Funding First supplemental - $4 million distributed by Cooperative Agreement (CA) Every state NPDN diagnostic lab receives $30-70k in cash and equipment annually All LGU s have access to CABI FY 04 budget - $3.7 million FY 05 budget - $4.5 million FY 06 budget - $5.0 million FY 07 budget - $5.0 million (Continuing resolution)

Vulnerable Agricultural Systems We need to protect our production systems from all introductions of high consequence pests and pathogens intentional or natural.

National Plant Diagnostic Network Pathway of Disaster Preparedness Detection Diagnosis What Mitigation infrastructure do we need? Control Recovery

National Plant Diagnostic Network University of California/Davis NPDN database Purdue University Kansas State University Michigan State University Provide Administrative Management Northeastern North-central Region Western Great Plains Region Establish Region Communications Region System Standardize and Certify Diagnostics Southern Region Coordinate First Detector Training Cornell University University of Florida

National Plant Diagnostic Network Operations Committee NPDN regional directors CSREES APHIS National Plant Board EDEN Regional IPM Centers

Regional Center Responsibility NPDN Regional Centers NPDN Regional Diagnostic Lab(s) National Operations Committee Regional Coordination Entomology National Committees Accreditation Plant Identification Communications Plant Pathology

biosafety cabinet real time PCR NPDN Regional Center Lab keypad lock security autoclave

Disease & Pest Management process detection diagnosis response Process must be rapid and accurate to minimize impact

National Plant Diagnostic Network NPDN program areas Detection & Diagnosis Education & Training Communications

National Plant Diagnostic Network NPDN program areas Detection & Diagnosis Education & Training Communications

National First Detector Training Program To facilitate rapid detection Who are First Detectors? Extension specialists Crop Advisors/Consultants Ag industry professionals Farmers Homeowners first detector

National Plant Diagnostic Network First Detector Training NPDN Curriculum Mission of NPDN Quality & secure sample submission High risk pests & pathogens Art & science of diagnosis Digitally-assisted diagnostics Exercise scenario program

National Plant Diagnostic Network NPDN program areas Detection & Diagnosis Education & Training Communications

Accurate Diagnosis if pathogen propagules are present

Collaborative Diagnostics Web Enabled Microscopes video conferencing image of unknown pathogen

serology Molecular and Chemistry -Based Diagnostics PCR real time PCR reduce diagnostic time (weeks to hours) increase accuracy of diagnosis

Diagnostic Process: NPDN Model Diagnostic Process: Current Model FD FD FD FD FD FD FD FD triage lab SPRO/SPHD parallel networked diagnostics triage lab to increase surge capacity triage lab Expert No surge capacity lab SPRO/SPHD SPRO/SPHD rapid identification of positives & rapid clearing of negatives triage lab SPRO/SPHD

National Plant Diagnostic Network NPDN program areas Detection & Diagnosis Education & Training Communications

National Plant Diagnostic Network Distributed communications networks in 50 states and 3 territories Communications NPDN Database Secure Communications Plant Diagnostic Information System

Scenario Exercise Training Objectives Practice operational functions in a nonemergency environment Relationship building Communications facilitation Engagement of all detection/response entities and agencies Improvement of protocols and processes

National Plant Diagnostic Network Rapid Outbreak Detection CN SH WA GL HM ST WH LG RA TH SC DC SD NT GH PL SM JW RP syndromic CD RO OB MC analysis RS DK WB GO TR EL SA EW MR common symptoms & hosts NS RH BT LY LE MP RC MN CS PN FI HG HV KE SF RN GW WD AL ED BU GY geographic FO delineation SG PR of affected area WL NO GT HS KW KM EK LC OT WA CY RL MS PT NM JA SN OS CF BR AT JF FR DP DG AN LV JO MI WY LN BB CR MT SV SW ME CA CM BA HP SU CL CT MG LB CK

Plant Diagnostics Information System Web Based GIS Reporting triage lab Rapid Outbreak Detection syndromic analysis triage lab triage lab

Plant Diagnostics Information System Web Based GIS Reporting identify area of vulnerability pathway prediction favorable environmental conditions target resources and mitigation efforts WIND HRP

National Plant Diagnostic Network Professional Scientific Societies Agricultural Professional Organizations Cultivating Partnerships