Plant Disease/Plant Pest Diagnostics in APHIS PPQ National Plant Diagnostic Network Annual Meeting Orlando, Florida January 2007
PPQ Western Region Plant Health Programs Center for Plant Health Science & Technology Emergency Domestic Programs Eastern Region
CENTER FOR PLANT HEALTH SCIENCE AND TECHNOLOGY National Science Programs NPPLAP Biological Control Unit National Plant Germplasm & Biotechnology Beltsville, MD Pest Detection Diagnostics & Edinburg, TX National Weeds Ft Collins, CO Analytical & Natural Products Chemistry Gulfport, MS Raleigh, NC Treatment Quality Assurance Unit Raleigh, NC Pest Survey Detection & Exclusion Otis, MA Decision Support & Pest Mgmt. Systems Phoenix, AZ Plant Epidemiology & Risk Analysis Raleigh, NC Fruit Fly Genetics & Waimanalo, HI Quality Unit Gulfport, MS Surveillance Technology Unit Fort Collins, CO As of: 01/10/2007
CPHST Functions Methods Development and Adaptation Methods Validation Training, including hands-on laboratory training Proficiency test development Emergency program support Molecular/biochemical diagnostics of certain non-routine samples Molecular diagnostics for certain plant pathogens, Asian gypsy moth, tephritids, others NPPLAP inspections Proficiency test delivery and evaluation
Planning Analysis & Regulatory co-ordination Plant Health Programs Program Data & Analysis Quarantine Policy Analysis & Support Phytosanitary Issues and Permits Registrations and Manuals Plant Safeguarding and Pest Identification Veterinary Regulatory support
Plant Safeguarding and Pest Identification APHIS Library National Identification Services Plant Germplasm and Quarantine Inspection & Policy Support Plant Inspection Station Support and Policy CITES Policy and support Post entry Quarantine Beltsville Inspection Station
PPQ and Cooperating National Identification Specialists for URGENTS/Domestics Cooperating National Identification Specialists Domestic Taxonomic/Diagnostics Coordinator Entomology Steve Passoa & Greg Evans NIS Systematic Entomology Lab (USDA-ARS-SEL) BARC & Smithsonian Nematology USDA-ARS BARC Weeds/Plants/Seeds Rodney Young, David Bitzel Beltsville, MD Mycology & Plant Pathology Dr. Mary Palm Dr. John McKemy Beltsville, MD Snails & Mollusks Dr. David Robinson Academy of Natural Sciences, PA Ticks Dr. James Mertins National Vet Services Lab, IA Honey Bees Carl Hayden Bee Res Center, AZ NIS also has access to services of about 75 port and survey identifiers to deal with emergency situations Example: Karnal bunt, PCN, EAB
NIS Functions - in support of domestic programs Coordination of all PPQ identification activities National identification authority - may authorize external specialists e.g., ALB, PSB National level identifications Insects, molluscs, nematodes, weeds, seeds, fungi, etc. Molecular Diagnostics Operational diagnostics - in support of CAPS, emergency programs, port interceptions P. ramorum, PCN, PPV, citrus canker, HLB, others Training
PPQ Molecular Diagnostic Lab Lab/offices located in Beltsville, Bldg. 580 Lab Director: Mary Palm Two scientists: Padma Sudarshana Gloria Abad Two support scientists March 2007
PPQ Diagnostics: CPHST and NIS Future Directions Morphological & molecular diagnostics are conducted with a dedication and record of continual improvement and quality assurance. Diagnostics are conducted with transparency and responsiveness to PPQ stakeholders and clients Diagnostics are readily available to PPQ programs Ramp-up of CPHST development and validation activities Diagnostics conducted for rapid response to stakeholders Creation of the PSPI Molecular Diagnostics Lab Diagnostic policies are in place to aid rapid response PPQ policy statement on PASS samples