Cancer genomics research Molecular profiling Ion AmpliSeq Comprehensive Cancer Panel Cat. No. 4477685 Ion AmpliSeq Made-to-Order Panels (Customize your own or browse redesigned community panels at ampliseq.com) Ion AmpliSeq On-Demand Panels (Order at ampliseq.com, for germline analysis) Ion AmpliSeq Cancer Hotspot Panel v2 Cat. No. A32914 (Chef-Ready) Ion AmpliSeq Library Kit Plus Ion AmpliSeq Library Kit 2.0 (96 LV format) Ion Torrent Oncomine Comprehensive Assay v3 Cat. No. A35806 (Chef-Ready) and Cat. No. A35805 (manual) Ion Torrent Oncomine Focus Assay* Cat. No. A35957 and A29230 Ion Torrent Oncomine BRCA Research Assay* Cat. No. A32841 (Chef-Ready) and Cat. No. A32840 (manual) Ion Xpress Barcode Adapters 1-96 Kit (Ion AmpliSeq Library Kit Plus recommended for Ion AmpliSeq On-Demand Panels) Ion Library Equalizer Kit Ion AmpliSeq Sample ID Panel Cat. No. 4479790 Ion AmpliSeq Kit for Chef DL8 Ion Chef Instrument Ion 510 /Ion 520 /Ion 530 Kit-Chef Ion 540 Kit-Chef Ion 550 Kit-Chef Ion OneTouch 2 System Ion 520 /Ion 530 Kit-OT2 Ion 540 Kit-OT2 Ion GeneStudio S5 System Ion GeneStudio S5 Plus System Ion GeneStudio S5 Prime System Ion 510 Chip Kit Ion 520 Chip Kit Ion 530 Chip Kit Ion 540 Chip Kit Ion 550 Chip Kit** Ion S5 Controls Kit Plus Ion S5 Calibration Standard Torrent Suite Software Ion Reporter Software (cloud or server) Torrent Suite Software Ion Reporter Software (cloud or server) Oncomine Knowledgebase Reporter Ion Torrent Oncomine Colon cfdna Assay* Cat. No. A31182 Liquid biopsy Ion Torrent Oncomine Pan-Cancer Cell-Free Assay* Cat. No. A37664 Ion Torrent Oncomine Lung Cell-Free Total Nucleic Acid Research Assay* Cat. No. A35864 Ion Torrent Oncomine Breast cfdna Research Assay v2* Cat. No. A35865 Ion Torrent Oncomine Immune Response Research Assay Cat. No. A32928 (Chef-Ready) and A32881 (manual) Immuno-oncology Ion AmpliSeq Immune Repertoire Assay Plus, TCR beta Cat. No. A35386 Ion Torrent Oncomine Tumor Mutation Load Assay* Cat. No. A37910 (Chef-Ready) and Cat. No. A37909 (manual) Heme-oncology Ion Torrent Oncomine Myeloid Research Assay* Cat. No. A36941 (Chef-Ready) and Cat. No. A36940 (manual) * To learn more about Oncomine solutions, go to thermofisher.com/oncomine-assays. Oncomine solutions are performance-verified on specific Ion Torrent chip configurations and workflows; contact a member of the clinical research team to learn more. All Chef-Ready configurations include the Ion AmpliSeq Kit for Chef DL8 containing barcodes and equalizer. Manual library preparation assays include library prep reagents. ** The Ion 550 Chip is only compatible with Ion GeneStudio Plus and Prime Systems.
Gene expression analysis Targeted RNA sequencing Whole-transcriptome RNA sequencing mrna sequencing Small-RNA analysis Ion AmpliSeq RNA Fusion Lung Cancer Research Panel Ion AmpliSeq Made-to-Order RNA Panels Ion AmpliSeq Library Kit Plus Ion Xpress Barcode Adapters 1-16 Kit Cat. No. 4471250 Ion AmpliSeq Transcriptome Human Gene Expression Kit Cat. No. A26325 (24 rxns), Cat. No. A26326 (96 rxns), Cat. No. A26327 (384 rxns) Ion AmpliSeq Transcriptome Mouse Gene Expression Kit Cat. No. A36555 (384 rxns) Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit Cat. No. A31446 (32 rxns) Ion AmpliSeq Transcriptome Mouse Gene Expression Panel, Chef-Ready Kit Cat. No. A36412 (32 rxns) rrna depletion: Invitrogen RiboMinus Eukaryote System v2 Cat. No. A15026 Invitrogen Low Input RiboMinus Eukaryote System v2 Cat. No. A15027 Sample controls: Invitrogen ERCC RNA Spike-In Mix Cat. No. 4456740 mrna isolation: Invitrogen Dynabeads mrna DIRECT Micro Purification Kit Cat. No. 61021 Sample controls: ERCC RNA Spike-In Mix Cat. No. 4456740 Invitrogen mirvana mirna Isolation Kit Cat. No. AM1560 Ion Total RNA-Seq Kit v2 Cat. No. 4475936 (12 rxns) and Cat. No. 4479789 (48 rxns) Or for less hands-on time: Ion Total RNA-Seq Kit for AB Library Builder System Cat. No. 4482416 Ion Total RNA Library Protocol Card for AB Library Builder System Cat. No. 4482563 Ion Xpress RNA-Seq Barcode 1-16 Kit Cat. No. 4475485 Check quality and quantity of RNA: Invitrogen Qubit 4 Fluorometer Cat. No. Q33226 Invitrogen Qubit RNA HS Assay Kit Cat. No. Q32852 Ion 520/Ion 530 Kit-OT2 Coverage Analysis Plugin Ion AmpliSeq RNA Plugin Third-party software (e.g., Partek software) Coverage Analysis Plugin Ion RNASeq Analysis Plugin Third-party software (e.g., Partek software) Ion AmpliSeq Small RNA Analysis Plugin
Inherited disease research Variant detection with targeted sequencing Variant detection with exome sequencing Ion AmpliSeq On-Demand Panels Ion AmpliSeq Made-to-Order Panels (Customize your own or browse predesigned panels at ampliseq.com) Ion AmpliSeq Exome RDY Kit 1x8 Cat. No. A38262 Ion AmpliSeq Exome RDY Kit 4x2 Cat. No. A38264 Ion AmpliSeq Library Kit Plus (Ion AmpliSeq Library Kit Plus recommended for Ion AmpliSeq On-Demand Panels) Ion AmpliSeq Sample ID Panel Cat. No. 4479790 Ion AmpliSeq library kits included in Ion AmpliSeq exome kits Check quality and quantity of dsdna: Invitrogen Qubit 4 NGS Starter Kit Cat. No. Q33228 (Includes Invitrogen Qubit 4 Fluorometer, Qubit dsdna HS Assay Kit, and Qubit assay tubes) Ion Xpress Barcode Adapters 1-16 Kit Cat. No. 4471250 Ion 520/Ion 530 Kit-OT2 Reproductive health research Preimplantation genetic screening (PGS) Ion SingleSeq Kit, included with any of the following: Ion ReproSeq PGS Kit with Ion 510 Chips Ion ReproSeq PGS Kit with Ion 520 Chips Ion ReproSeq PGS Kit with Ion 530 Chips Ion ReproSeq PGS Kit with Ion 510 Chips Ion ReproSeq PGS Kit with Ion 520 Chips Ion ReproSeq PGS Kit with Ion 530 Chips Ion ReproSeq PGS Kit with Ion 510 Chips Ion ReproSeq PGS Kit with Ion 520 Chips Ion ReproSeq PGS Kit with Ion 530 Chips Ion Reporter Software aneuploidy workflow
Infectious disease research Discovery and characterization Targeted sequencing Metagenomics Ion AmpliSeq Made-to-Order Panels (Customize your own panel for any genome, or select from the following predesigned panels at ampliseq.com) Ion AmpliSeq TB Research Panel Ion AmpliSeq Ebola Research Panel Ion Xpress Plus Fragment Library Kit Cat. No. 4471269 Thermo Scientific MuSeek Library Preparation Kit, Ion Torrent system compatible Ion Total RNA-Seq Kit v2 Cat. No. 4475936 Automated library preparation: Ion AmpliSeq Transcriptome Human Gene Expression Kit Cat. No. A26325 (24 rxns), Cat. No. A26326 (96 rxns), Cat. No. A26327 (384 rxns) Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-Ready Kit Cat. No. A31446 (32 rxns) Ion 16S Metagenomics Kit Cat. No. A26216 Ion 16S Metagenomics Kit Cat. No. A26216 Ion Xpress Plus Fragment Library Kit Cat. No. 4471269 (For microbial community characterization by 16S rdna sequencing) MuSeek Library Preparation Kit, Ion Torrent system compatible Ion Library TaqMan Quantitation Kit Cat. No. 4468802 OPTONAL or other Ion Xpress Barcode Adapters Kits Cat. Nos. A34019 and A3446 For 600 bp (longer-read) sequencing: Ion 520 & Ion 530 ExT Kit-Chef Cat. No. A30670 (Template preparation and sequencing reagents for 400- to 600-base read libraries) Ion 520/530 Kit-OT2 Ion S5 ExT Calibration Standard Cat. No. A30738 Ion Torrent E. coli Control 600 Library Cat. No. A32470 Assembler SPAdes v3.1 Plugin Ion 16S Metagenomics workflow
Infectious disease research (continued) Plant and animal genomics research Bacterial and viral typing research Variant detection Design custom panels for any reference genome using the Ion AmpliSeq Designer at ampliseq.com; upload a custom reference or use one of the preloaded reference genomes: mouse, chicken, cow, pig, sheep, Chinese hamster, dog, maize, rice, soybean, or tomato MuSeek Library Preparation Kit, Ion Torrent system compatible Ion Xpress Plus Fragment Library Kit Cat. No. 4471269 (or other kits for library preparation from genomic DNA) Ion Library TaqMan Quantitation Kit Cat. No. 4468802 other Ion Xpress Barcode Adapters Kits Ion AmpliSeq LIbrary Kit 2.0 (96 LV format) Automated library preparation: Cat. Nos. A34019 and A3446 For 600 bp (longer-read) sequencing: Ion 520 & Ion 530 ExT Kit-Chef Cat. No. A30670 (Template preparation and sequencing reagents for 400- to 600-base read libraries) Ion 520/530 Kit-OT2 Ion S5 ExT Calibration Standard Cat. No. A30738 E. coli Control 600 Library Cat. No. A32470 Pathogen Detector Plugin Applied Biosystems ABL ViroScore-HIV application subscription For Research Use Only. Not for use in diagnostic procedures. 2015 2018 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. Partek is a trademark of Partek Incorporated. COL32360 0318