THE UNIVERSITY OF TEXAS AT EL PASO COLLEGE OF SCIENCE DEPARTMENT OF BIOLOGICAL SCIENCES SYLLABUS Course #: MICR 4453 Course Title: Immunology CRNs: 12950, 12952, 12953, 15016, 17668 Credit Hrs: 4.0 Term: Fall 2011 Course Meetings & Location: Lectures: Physical Science bldg 208 Labs: Biology bldg B408 and B411 Time: Lectures: 8:30 9:20 am Labs: see lab schedule below Days: Lectures: Mon, Wed, and Fri (MWF) Labs: Mon, Tue, Thu, Sat (MTRS) Prerequisite Courses: MICR 2440 and CHEM 3325 and CHEM 3125. MICR 4453 replaced MICR 3453-1454. Course Fee: (if applicable) Laboratory fee required. Instructors: Dr. Igor C. Almeida, PhD, Professor, Dept. of Biol. Sciences (Lectures) Dr. Rosa A. Maldonado, PhD, Associate Professor, Dept. of Biol. Sciences (Labs) Office Location: Department of Biological Sciences Biosciences Bldg., room 5.124 (Dr Almeida) Biosciences Bldg., room 5.132 (Dr Maldonado) Contact Info: Phone: 747-6086 (Dr Almeida) 747-6891 (Dr Maldonado) E-mail: icalmeida@utep.edu (Dr Almeida) ramaldonado@utep.edu (Dr Maldonado) Fax: 747-5808 Emergency Contact: Dr Alexandre F. Marques, 747-6898; afmarques@utep.edu Dr Esteban M. Cordero Veas, 747-6898; emcorderoveas@utep.edu Office Hrs: Mon, Wed, and Fri, 10:00-11:00 am, or by appointment. Textbook(s), Materials: Required: Kindt, Thomas J., Osborne, Barbara A., Goldsby, Richard A. Kuby s Immunology, 6th edition, 2007 (corrected version), W.H. Freeman ISBN: 1-4292-0211-4; ISBN-13: 978-1-429-20211-4 Textbook s homepage: http://www.whfreeman.com/newcatalog.aspx?disc=biology&course=i mmunology&isbn=1429202114 Suggested: Immunology books, tutorials, articles, and reviews available over the Internet. Course Objectives (Learning Outcomes): The course is designed for undergraduate students pursuing Biology, Microbiology, and Biomedical-related major degrees. The course is also offered to graduate (MS, PhD) students. The main objective of the course is to provide the students a comprehensive view of the immune system and how it works in health and disease. We will focus our attention in basic mechanisms driving the innate and adaptive immune responses against foreign organisms and molecules. In addition, we will study the molecular mechanisms that trigger and sustain autoimmune and malignant (cancer) processes. Last, but not least, we do hope this course may create or enhance the students interest in basic and applied research in Immunology. Course Period: Activities/Assignments: Aug 22, 2011 - Dec 1, 2011 1) Lectures: Physical Science Bldg 208 Lecture s days and time: Mon/Wed/Fri, 8:30-9:20 am Lecture attendance is required. 2) Laboratories: Biology building B408 and B411 Lab days and times: Section Day, Time Where 17668 Mon, 2:00 pm 4:50 pm Biology bldg B408 12952 Mon, 5:00 pm 7:50 pm Biology bldg B408 12953 Tue, 12:00 pm 2:50 pm Biology bldg B411 12950 Thu, 12:00 pm 2:50 pm Biology bldg B411 15016 Sat, 4:30 pm 7:20 pm Biology bldg B408 Lab attendance is required. 3) Quizzes: On-line (Blackboard) 4) Seminars/Presentations: see schedule below.
Grading Policy: 2 1) Two 100-point exams and one 100-point final (comprehensive) exam will be given during the course. Altogether, they are worth 3/5 or 60% of the final grade. Regardless their average grade on Exams 1 and 2, all students must take the Final Exam, which will cover all topics given/discussed during the course. 2) One seminar (1/5 or 20% of the final grade) must be presented by the students in groups of maximum 6 students (minimum 5) (see seminar schedule below). Seminar attendance and presentation are mandatory. 3) The remaining 1/5 or 20% of the final grade will be provided by the lab grade. 4) Quizzes (total of 10) will add 10 extra-points (1.0 per quiz) to the total number of points. a) Make-up quizzes or exams will not be given under normal circumstances (please refer to Make-up Policy below for more details and exceptions) b) The Final Exam s grade may replace a missing grade (due to absence) on Exam 1 or 2. Grade calculation: Points % Exam #1 (Sep 19 th, Mon, 8:30-9:20 am).100 20 Exam #2 (Oct 17 th, Mon, 8:30-9:20 am)..100 20 Final Exam (Dec 5 th, Mon, 10:00 am-12:45 pm) 100 20 Group Seminar/Presentation 100 20 Laboratory 100 20 Total : 500 100 1) The Final Exam s grade may replace a missing grade (due to absence) on Exam 1 or 2. 2) Quizzes: Maximum 10 extra-points (1.0 point per quiz) to be added to the Total number of points. Final number of points Final Grade 451 500 A 401 450 B 351 400 C 201 350 D <201 F Requirements and Grading for Graduate Students: Graduate (MS or PhD) students are not required to attend labs. Therefore, in this case, the final grade will be calculated as follows: Points % Exam #1 (Sep 19 th, Mon, 8:30-9:20 am).100 20 Exam #2 (Oct 17 th, Mon, 8:30-9:20 am)..100 20 Final Exam (Dec 5 th, Mon, 10:00 am-12:45 pm) 100 20 Group Seminar/Presentation.200 40 Total : 500 100 1) The Final Exam s grade may replace a missing grade (absence) on Exam 1 or 2. 2) Quizzes: Maximum 10 extra-points (1.0 point per quiz) to be added to the total number of points. Make-up Policy: Attendance Policy: Make-up exams and quizzes will NOT be given under normal circumstances. However, if you have to miss an exam or quiz due to serious medical or family reason, military duties, or any other extraordinary reason, please contact Dr Almeida or Dr Maldonado at your earliest convenience to arrange a make-up exam/quiz. Classes, seminars, and labs are required for all undergraduate students. Classes and seminars but not labs are required for graduate students. Absences must be communicated at least 24 hours in advance. In case of an emergency, please contact the instructor(s) by e-mail and/or phone at your earliest convenience. 2
Academic Integrity Policy: Civility Statement: Disability Statement: Military Statement: 3 For UTEP's Policy on academic dishonesty, please refer to: http://admin.utep.edu/default.aspx?pagecontentid=2084&tabid=30292 http://studentaffairs.utep.edu/portals/234/appendix%20a%20discipline%20process%2 0NEW%20OSL%202009.pdf Students are encouraged to actively participate in all course activities. However, during lectures and labs, use of cell phone, talking, and other activities unrelated to the course are not acceptable. If a student has or suspects he/she has a disability and needs an accommodation, he/she should contact the Disabled Student Services Office (DSSO) at 747-5148 or at <dss@utep.edu> or go to Room 106 Union East Building. The student is responsible for presenting to the instructor any DSS accommodation letters and instructions. Website: http://studentaffairs.utep.edu/default.aspx?alias=studentaffairs.utep.edu/dsso If you are a military student with the potential of being called to military service and /or training during the course of the semester, you are encouraged to contact the instructor by phone and/or e-mail at the earliest convenience. Course Schedule: Lecture days and time: Mon/Wed/Fri, 8:30-9:20 am Lecture room: Physical Science bldg room 208 Laboratories: Biology building B408 and B411 Lab days and times*: Section Day, Time Where 17668 Mon, 2:00 pm 4:50 pm Biology bldg B408 12952 Mon, 5:00 pm 7:50 pm Biology bldg B408 12953 Tue, 12:00 pm 2:50 pm Biology bldg B411 12950 Thu, 12:00 pm 2:50 pm Biology bldg B411 15016 Sat, 4:30 pm 7:20 pm Biology bldg B408 * Note: There will be no labs on the first week of classes. Labs will start on Aug 29 th (for CRNs 17668 and 12952), Aug 30 th (for CRN 12953), Sep 1 st (for CRN 12950), and Sept 3 rd (for CRN 15016). Exam # Date Time Location Exam #1 Sep 19 th, Mon 8:30-9:20 am PHYS 208 Exam #2 Oct 17 th, Mon 8:30-9:20 am PHYS 208 Final Exam Dec 7 th, Mon 10:00 am-12:45 pm PHYS 208 # Note: Students must bring their own scantron and pencil for each exam, since these will not be provided by the instructor. Quizzes: They will be posted on-line at the course s website (Blackboard). Students will be given a notice in advance. 3
4 Seminar Group # * 1 Oct 31, Mon 2 Oct 31, Mon 3 Nov 2, Wed 4 Nov 4, Fri 5 Nov 7, Mon Schedule of Students Seminars/Presentations Date Topic # Sub-Topic Tolerance and Autoimmunity (Chapter 16) Transplantation Immunology (Chapter 17) -Establishment and maintenance of tolerance 6 Nov 7, Mon -Clinical transplantation 7 Nov 9, Wed 8 Nov 11, Fri Immune Response to -Bacterial infections 9 Nov 11, Fri Infectious Diseases -Parasitic infections 10 Nov 14, Mon (Chapter 18) -Fungal diseases -Organ-specific autoimmune disease -Systemic autoimmune diseases -Animal models for autoimmune diseases -Role of CD4+ T cell, MHC, and TCR in autoimmunity -Proposed mechanisms for induction of autoimmunity -Immunologic basis of graft rejection -Clinical manifestations of graft rejection -General and specific immunosuppressive therapies -Immune tolerance to allografts -Viral infections 11 Nov 14, Mon -Emerging infectious diseases 12 Nov 16, Wed -Active and passive immunization -Designing vaccines for active immunization -Vaccine adjuvants 13 Nov 16, Wed Vaccines (Chapter 19) -Live/attenuated and inactivated/killed vaccines 14 Nov 18, Fri -Subunit and conjugate vaccines 15 Nov 18, Fri -DNA vaccines 16 Nov 21, Mon -Primary immunodeficiencies AIDS and Other 17 Nov 23, Wed Immunodeficiencies -AIDS and other acquired or secondary immunodeficiencies 18 Nov 23, Wed (Chapter 20) -Current therapies to treat AIDS -Vaccine to prevent AIDS: update 19 Nov 28, Mon -Cancer: Malignant transformation of cells -Oncogenes and cancer induction 20 Nov 28, Mon Cancer and the -Tumors of the Immune system Immune System -Tumor antigens 21 Nov 30, Wed (Chapter 21) -Tumor evasion 22 Nov 30, Wed -Cancer Immunotherapy * Groups will be assigned at the beginning of the course. Seminar attendance is mandatory. For every unjustified absence, 5 points will be taken off the absentee s presentation grade. # Textbook: Kuby s Immunology, 6th edition, 2007 4
5 Course Program: Part I Introduction 1. Overview of the Immune System 2. Cells and Organs of the Immune System 3. Innate Immunity Part II Generation of B-Cell and T-Cell Receptors 4. Antibodies and Antigens 5. Organization and Expression of Immunoglobulin Genes 6. Antigen-Antibody Interactions: Principles and Applications 7. The Complement System 8. The Major Histocompatibility Complex (MHC) and Antigen Presentation 9. T-Cell Receptor 10. T-Cell Maturation, Activation, and Differentiation 11. B-Cell Generation, Activation and Differentiation Part III Immune Effector Mechanisms 12. Cytokines 13. Leukocyte Activation and Migration 14. Cell-Mediated Cytotoxic Responses 15. Hypersensitive Reactions Part IV The Immune System in Health and Disease 16. Tolerance and Autoimmunity 17. Transplantation Immunology 18. Immune Responses to Infectious Diseases 19. Vaccines 20. AIDS and Other Immunodeficiencies 21. Cancer and the Immune System 5
Laboratory Schedule MICR4453 - Fall 2011 6 Lab attendance is required. Absence from the lab will be excused ONLY if notice is given to the instructor 24 hours in advance. Otherwise, you will receive 1 point off your grade for each unexcused lab absence. Make every effort to arrive on time. Points LABORATORY GRADING: Lab reports (5): 30 Quizzes: 20 Exams 1 and 2: 50 Total: 100 LABORATORY REPORT The laboratory reports will be prepared in groups. It should contain title, objectives, methodology, results, discussion, and references. Read this section carefully. For each lab exercise your lab report should contain the following: I. Title Brief and descriptive. Example: Lab Exercise # 5 - Handling of Animals, Immunizations II. Introduction It describes the purpose of the lab exercise/experiment and includes background information presented by the instructor or included in the handout. It should have a minimum of 2 paragraphs in length. III. Materials and Methods It should describe the supplies, reagents and equipment used and how the experiment or technique was performed. It should be sufficiently detailed to permit another person to reproduce the work. IV. Results A written account of observations, findings, and raw data. The Results section should also include data in the form of drawings, graphs or tables, when appropriate. Drawings, tables, and graphs should be numbered and have their own brief titles. V. Discussion A summary of what was learned from both methodological and principle standpoints. Also, you should describe problems encountered and possible solutions. QUIZZES: Before each lab there will be a quiz on the exercise/experiment to be carried out. SCHEDULE: Where: Biology building B408 and B411 Days and times*: Section (CRN) Day, Time Where 17668 Mon (M), 2:00 pm 4:50 pm Biology bldg B408 12952 Mon (M), 5:00 pm 7:50 pm Biology bldg B408 12953 Tue (T), 12:00 pm 2:50 pm Biology bldg B411 12950 Thu (R), 12:00 pm 2:50 pm Biology bldg B411 15016 Sat (S), 4:30 pm 7:20 pm Biology bldg B408 * Note: There will be no labs on the first week of classes. Labs will start on Aug 29 th, Mon (for CRNs 17668 and 12952); Aug 30 th, Tue (for CRN 12953); Sep 1 st, Thu (for CRN 12950), and Sept 3 rd, Sat (for CRN 15016). 6
7 Week Date (Day) Lab # EXPERIMENT ASSIGNMENT 1 8/29 (M), 8/30 (T), 9/1 (R), 9/3 (S) 1 Laboratory safety - 2 9/12 (M), 9/13 (T), 9/15 (R), 9/17 (S) 2 Hematocytometer and cell counter - 3 9/19 (M), 9/20 (T), 9/22 (R), 9/24 (S) 3 White cell staining Report 1 4 9/26 (M), 9/27 (T), 9/29 (R), 10/1 (S) 4 Phagocytes - 5 10/3 (M), 10/4 (T), 10/6 (R), 10/8 (S) 5 Handling of animals, immunizations Report 2 6 10/10 (M), 10/11 (T), 10/13 (R), 10/15 (S) - Exam 1-7 10/17 (M), 10/18 (T), 10/20 (R), 10/22 (S) 6 ELISA Report 3 8 10/24 (M), 10/25 (T), 10/27 (R), 10/29 (S) 7 Start western blotting - 9 10/31 (M), 11/1 (T), 11/3 (R), 11/5 (S) 8 Finish western blotting Report 4 10 11/7 (M), 11/8 (T), 11/10 (R), 11/12 (S) 9 Dry lab - 11 11/14 (M), 11/15 (T), 11/17 (R), 11/19 (S) - Exam 2 Report 5 7