Institute of Science & Technology in Medicine Control of Substance Hazardous to Health COSHH assessment Title of Eperiment/Procedure: Rat/Mouse PINP EIA (commercial kit from IDS. AC-33F1) Date of Assessment: 27/05/08 Names & Status of Worker: Sarah Rathbone PhD Name of hazardous substances to be used 1. hydrochloric acid (Stop Solution) Type of Hazard Chemical or Biological Hazards identified NOTE: enter skin contact or absorption, eye contact, inhalation, injection, ingestion, where relevant. Chemical Hazard. Irritating to eyes and skin, swallowed. Approimate quantity and the likelihood of eposure, e.g. high, medium, low Low. 14ml of 0.5M HCL. 2. Tetramethylbenzidine (TMB Substrate Solution). 3. Sodium Azide (in Calibrators & Controls) 4. Rabbit anti-pinp antibody (coated onto plate). 5. Enzyme conjugate. 6. Calibrators 7. Control standards 8.Wash buffer 9. Sample diluent Chemical Hazard. Skin contact, eye contact, swallowed. Biological Hazard. Skin contact, injection, eye contact. Biological Hazard. Skin contact, injection, Chemical & Biological Hazard. Skin contact, injection, Chemical & Biological Hazard. Skin contact, injection, Medium. 24 ml with hydrogen peroide. Low. <1% used as preservatives for controls & calibrators in kit. Low. Low. In phosphate buffered saline (PBS). Low. 0.5 ml Mouse & goat serum in phosphate buffered saline and <1% sodium azide. Low. 0.5 ml Rat serum in PBS and <1% sodium Azide. Low. 50ml PBS with Tween. Low. 20ml PBS containing 0.05% sodium azide.
Overall risk assessment of the substances Very high High Medium Low Carcinogen category
1. Hydrochloric acid (Stop Solution) 2. Tetramethylbenzidine (TMB Substrate Solution). 3. Sodium Azide (in Calibrators & Controls) 4. Rabbit anti-pinp antibody (coated onto plate) 5. Enzyme conjugate. 6. Calibrators 7. Control standards 8.Wash buffer After swallowing, wash mouth out with water & call physician. Risk phrase - R36/38. Accidental spillage absorb with wadding & dispose of in yellow clinical waste bag. Wear suitable Risk phrase R21/22. Accidental spillage absorb with wadding & dispose of in yellow clinical waste bag. Wear suitable After swallowing, wash mouth out with water, call physician and show the label. In case of Risk phrase - R22. Accidental spillage absorb with wadding & dispose of in yellow clinical waste bag. Wear suitable Accidental spillage absorb with Accidental release Biohazard 1: Absorb spillages with wadding. Discard as clinical waste (yellow bags). Disinfect spillage areas & contaminated equipment. Wear suitable protective clothing, gloves & eye protection. Accidental release Biohazard 1: Absorb spillages with wadding. Discard as clinical waste (yellow bags). Disinfect spillage areas & contaminated equipment. Wear suitable protective clothing, gloves & eye protection. Accidental spillage absorb with
9. Sample diluent Accidental spillage absorb with Information source: Immunodiagnostic systems Ltd (IDS Ltd). Product code AC-33F1. Tel: 0191 519 0660 Institute of Science & Technology in Medicine Control of Substance Hazardous to Health COSHH assessment Biological Safety Cabinet Control Measures adopted (personal protective equipment) Fume cupboard Good Ventilation Safety Goggles Gloves Face Mask/ If special, state details Dust mask Gloves Spillage Face protection Disinfectant Spillage kit Mop up Mi with sand Method of Disposal Chemical Waste bottle Drain Yellow bag Autoclave No hazard Emergency procedures In case of fire In case of water failure In case of electricity failure Emergency contact name N/A all solutions are water based. Eperiment should not be carried out as water must be available in case of accidental contact of substances with skin. Eperiment should not be carried out as biological cabinet would be inoperative. Jackie Rathbone
Emergency phone number 01270 Assessor Supervisor or COSHH Supervisor Signatures Name and signature Sarah Cartmell Date