BioResearch. Nucleic Acid Electrophoresis The Standard in Quality, Precision and Performance

BioResearch Nucleic Acid Electrophoresis The Standard in Quality, Precision and Performance

BioResearch Nucleic Acid Electrophoresis Content Introduction 3 FlashGel System 4 6 Reliant Minigels 7 8 Latitude HT Gels and Midigels 9 10 Agarose 11 13 Ladders and Markers for DNA and RNA 14 15 Stains 16 AccuGENE Buffers 17 GelBond Film 19 2

Lonza is a leading manufacturer and trusted supplier of agarose. With over 50 years of experience in nucleic acid electrophoresis, we bring a strong history of innovation and reliability to your most important research. Our well known product brands set the standard in quality, purity and performance for electrophoresis. FlashGel System Reliant and Latitude Precast Gels SeaKem, NuSieve and MetaPhor Agarose SimplyLoad DNA Ladders GelStar and SYBR Stains AccuGENE Buffers GelBond Gel Support Film 3

BioResearch Nucleic Acid Electrophoresis FlashGel System Rapid DNA/RNA Electrophoresis with Real Time Results in Minutes The FlashGel System provides an ultra-fast, hands free DNA/RNA separations and DNA recovery device with supporting reagents, portable for any bench top and easy to use with no gel preps. This includes all-in-one separations, recovery of DNA, real time visualization, capturing images without UV light, and software to download images onto your laptop/cell phone. What the FlashGel System can do for you: DNA separation in 5 minutes Recover DNA samples directly from the cassette, without band excision or purification in 10 minutes Separate RNA in 30 minutes, with no hazardous reagents or contaminating RNases System Devices (FlashGel Dock, Camera, Power Supply, Precast Cassettes, Loading Dye and Markers) FlashGel for DNA Separation Powerful and compact device with precast gel cassettes for separating nucleic acids ranging from 50 bp to 4 kb. DNA separation in 5 minutes, see bands migrate instantly Photograph gels at the bench, without DNA damaging UV light, and document Detect <0.1 ng DNA with outstanding sensitivity and resolution, up to 20 times more sensitive than ethidium bromide www.flashgel.com DNA bands as viewed during a run on the FlashGel Dock. 4

FlashGel for DNA Recovery Easy DNA recovery with no band excision required. Separate and recover DNA in 10 minutes Recover DNA samples directly from the cassette, without band excision or purification Visualize DNA recovery without UV light, and obtain efficiency of 80% 100% Work Flow Conversion, Time and Method Savings FlashGel Recovery System Column Recovery Method FlashGel for RNA Separation Fast and easy qualitative check for RNA separation. Separate RNA in less than 30 minutes at the bench Detect <10 ng RNA per band with outstanding sensitivity and resolution No hazardous reagents or contaminating RNases 1 2 3 4 Separate fragments on FlashGel System 3 5 minutes Recover DNA directly from wells 1 5 minutes Total time: 4 10 minutes Separate fragments on agarose gel 30 60 minutes Excise bands from agarose gel 5 10 minutes Recover DNA via spin column 25 30 minutes Total time: 1 hour Samples of RNA Marker (Lonza) (lanes 1 and 2) and E. coli total RNA (lanes 3 and 4) contain 50 ng (lanes 1 and 3) or 250 ng (lanes 2 and 4) of RNA per 5 µl load. Samples prepared with Formaldehyde Sample Buffer (Lonza) and denatured 5 minutes at 65 C. FlashGel RNA Cassette run for 8 minutes, followed by a 20 minute hold prior to imaging. FlashGel Recovery Cassette, Easy for Recovery Load sample here Extract DNA here 5

BioResearch Nucleic Acid Electrophoresis Ordering Information FlashGel System Cat. No. Description Size FlashGel System 57025 FlashGel Dock Dock only 57040 FlashGel Camera Camera only 57068 FlashGel Power Supply Power supply only 57067 FlashGel System Dock, Camera and DNA starter kit 57062 FlashGel Device Pack Includes FlashGel Dock, FlashGel Power Supply and FlashGel Camera 57065 FlashGel Camera Pack Includes FlashGel Dock and FlashGel Camera 57069 FlashGel Power Supply Pack Includes FlashGel Dock and FlashGel Power Supply FlashGel System for DNA Separation and Recovery 57023 FlashGel DNA Cassettes 9/pk 1.2%, 12+1 single tier 57029 FlashGel DNA Cassettes 9/pk 1.2%, 16+1 double tier (34-well) 57031 FlashGel DNA Cassettes 9/pk 2.2%, 12+1 single tier 57032 FlashGel DNA Cassettes 9/pk 2.2%, 16+1 double tier (34-well) 57051 FlashGel Recovery Cassettes 9/pk 1.2% agarose, 8+1 double tier (18-well) 57022 FlashGel Recovery Cassettes 9/pk 2.2% agarose, 8+1 double tier (18-well) 57060 FlashGel Recovery Buffer 2 500 μl 50462 FlashGel Loading Dye (5X) 5 1 ml vials 5X concentration 50475 FlashGel QuantLadder, 100 bp 1.5 kb Multi-marker 57033 FlashGel DNA Marker, 50 bp 1.5 kb Band Sizes: 50/100/150/200/300/500/800/1500 bp 57034 FlashGel DNA Marker, 100 bp 3 kb Band Sizes: 100/300/500/800/1500/3000 bp 50473 FlashGel DNA Marker, 100 bp 4 kb Band Sizes: 100/200/300/500/800/1250/2000/4000 bp 57063 FlashGel DNA Kit, includes FlashGel DNA Cassettes, FlashGel Loading Dye, and FlashGel Marker 100 bp 4 kb 57064 FlashGel Recovery Kit, includes FlashGel Recovery Cassettes, FlashGel Recovery Buffer, FlashGel Loading Dye, FlashGel QuantLadder and Glasses FlashGel System for RNA Separation and Recovery 1.2%, 12+1-well single tier 9/pk 1.2%, 8+1-well double tier 9/pk 57027 FlashGel RNA Cassettes 9/pk 1.2% agarose, 12+1 single-tier 57028 FlashGel RNA Cassettes 9/pk 1.2% agarose, 16+1 double-tier (34-well) 50577 FlashGel RNA Marker, 0.5 kb 9 kb 0.5 bp 9 kb 50 μg (1 μg/ml) 50462 FlashGel Loading Dye (5X) 5 1 ml vials 5X concentration 57024 FlashGel RNA Kit, includes FlashGel RNA Cassettes, RNA Marker, Sample Buffer and Molecular Biology Water 1.2%, 12+1-well single tier 9/pk 6

Reliant Minigels Convenient Precast Gels for DNA/RNA Separation and Recovery Reliant Gels are precision manufactured for reproducible resolution of DNA sizes from 8 bp to 10 kb and designed for verification of RNA integrity and are guaranteed RNase-free. Made with high quality SeaKem and NuSieve Agarose Compatible with most minigel chambers Versatile format options DNA Gel Formats RNA Gels Formats 8-well 12-well 8-well 20-well 6 cm 9.5 cm 9.5 cm 9.5 cm 9.5 cm 6 cm 24-well 6 cm 20-well 6 cm 6 cm 9.5 cm 9.5 cm 6 cm 7

BioResearch Nucleic Acid Electrophoresis Ordering Information Reliant Minigels for DNA (20 gels/box) Cat. No. Description Buffer Separation Range (bp) Agarose 8-well 54801 Reliant Gel no stain with ethidium bromide (0.5 μg/ml) TAE 400 10,000 1% SeaKem Gold Agarose 54803 with ethidium bromide (0.5 μg/ml) TAE 400 10,000 1% SeaKem Gold Agarose 54925 TAE 20 1,000 4% NuSieve 3:1 Plus Agarose 54903 TBE 300 8,000 1% SeaKem Gold Agarose 54927 TBE 8 1,000 4% NuSieve 3:1 Plus Agarose 12-well 54821 Reliant Gel with ethidium bromide (0.5 μg/ml) TAE 400 10,000 1% SeaKem Gold Agarose 54820 TBE 300 8,000 1% SeaKem Gold Agarose 54825 TBE 100 3,000 2% SeaKem Gold Agarose 54823 TBE 8 1,000 4% NuSieve 3:1 Plus Agarose 24-well 54905 Reliant Gel with ethidium bromide (0.5 μg/ml) TBE 300 8,000 1% SeaKem Gold Agarose 54813 TBE 100 3,000 2% SeaKem Gold Agarose 54929 TBE 8 1,000 4% NuSieve 3:1 Plus Agarose 20-well 54938 Reliant Gel no stain TBE 100 3,000 2% SeaKem Gold Agarose 54944 TBE 8 1,000 4% NuSieve 3:1 Plus Agarose 54907 with ethidium bromide (0.5 μg/ml) TBE 300 8,000 1% SeaKem Gold Agarose 54939 TBE 100 3,000 2% SeaKem Gold Agarose 54928 TBE 8 1,000 4% NuSieve 3:1 Plus Agarose Ordering Information Reliant Minigels for RNA Cat. No. Description Size Buffer Agarose 54922 Reliant RNA Gel 20 minigels/box 54948 20-well landscape 8-well MOPS 1.25% SeaKem Gold MOPS 1.25% SeaKem Gold 8

Latitude HT Gels and Midigels Precision Precast Gels for DNA/RNA Separations in High-throughtput Format Latitude DNA Gels are specifically designed for high sample throughput for DNA ranging 8 bp to 10 kb, and Latitude RNA Gels are RNase-free and optimal for verification of RNA integrity. Made with high quality SeaKem and NuSieve Agarose Fits in most large submerged electrophoresis systems and is compatible with multichannel pipetters Supports DNA well formats from 20 200 wells and RNA from 20 40 wells DNA HT Gel formats DNA/RNA Midigel formats 2 x 50 100-well 4 x 25 100-well 20-well 40-well 14 cm 14 cm 15 cm 15 cm 24 cm 24 cm 4 x 50 200-well 10 cm 10 cm 14 cm 24 cm 9

BioResearch Nucleic Acid Electrophoresis Ordering Information Latitude HT Gels (5 gels/box) Cat. No. Description Buffer Separation Range (bp) Agarose 2 50-wells, 100-well 57206 Latitude HT Precast Gel multichannel pipette compatible (alternate well) contains ethidium bromide (0.5 μg/ml) TAE 100 3,000 2% SeaKem LE Plus Agarose 57225 TBE 8 1,000 4% NuSieve 3:1 Plus Agarose 57226 TBE 100 2,000 2% SeaKem LE Plus Agarose 4 25-wells, 100-well 57246 Latitude HT Precast Gel multichannel pipette compatible (consecutive well) contains ethidium bromide (0.5 μg/ml) TBE 100 2,000 2% SeaKem LE Plus Agarose 57255 TBE 8 1,000 4% NuSieve 3:1 Plus Agarose 4 50-wells, 200-well 57214 Latitude HT Precast Gel multichannel pipette compatible (alternate well) contains ethidium bromide (0.5 μg/ml) TAE 400 10,000 1% SeaKem LE Plus Agarose 57234 TBE 300 8,000 1% SeaKem LE Plus Agarose 57235 TBE 8 1,000 4% NuSieve 3:1 Plus Agarose 57236 TBE 100 2,000 2% SeaKem LE Plus Agarose Ordering Information Latitude Midigels (8 gels/box) Cat. No. Description Buffer Separation Range (bp) Agarose 20-well 57200 Latitude Midigel contains ethidium bromide (0.5 μg/ml) TAE 400 10,000 1% SeaKem LE Plus Agarose 57220 TBE 300 8,000 1% SeaKem LE Plus Agarose 40-well 57210 Latitude Midigel contains ethidium bromide (0.5 μg/ml) TAE 400 10,000 1% SeaKem LE Plus Agarose 57230 TBE 300 8,000 1% SeaKem LE Plus Agarose 57231 TBE 100 2,000 2% SeaKem LE Plus Agarose 57232 TBE 8 1,000 4% NuSieve 3:1 Plus Agarose 57211 TAE 100 3,000 2% SeaKem LE Plus Agarose Ordering Information Supporting Products Cat. No. Description Size 50560 Glyoxal Sample Buffer ideal for Northern blotting 50571 Formaldehyde Sample Buffer ideal for visualization/analysis contains bromophenol blue and xylene cyanol 56990 Latitude Midigel Chamber casting accessories not available gel chamber 56988 TruBand Gel Anchor free with your first order of Latitude Gels 56989 TruBand Gel Anchor free with your first order of Latitude Gels 1.7 ml 5 1 ml Gel chamber Latitude Chamber Standard chambers 10

Agarose Consistency and Reliability to Deliver the Results You Need Lonza s agarose is designed for optimal resolution, blotting and recovery. Selecting the right agarose for your application will minimize errors, optimize results and even reduce costs. With a full range of agarose types designed by fragment size, sample type and application, we can help with any electrophoresis method. Higher performance in resolution and band sharpness Consistent and accurate results with high quality Greater gel strength for easy handling Choose the Right Agarose for Your Needs Agarose and Applications SeaKem Gold Agarose PFGE, Large fragment analysis 1 kb 10 Mb GTG SeaPlaque GTG Agarose In-gel reactions, DNA and RNA recovery, cloning 200 bp 25 kb GTG SeaPlaque Agarose DNA and RNA recovery, cloning 200 bp 25 kb SeaKem GTG Agarose DNA and RNA recovery, cloning 100 bp 23 kb GTG SeaKem LE Agarose Routine analysis, blotting 100 bp 23 kb NuSieve GTG Agarose DNA and RNA recovery, cloning 50 bp 1 kb GTG NuSieve 3:1 Agarose PCR blotting 50 bp 1 kb MetaPhor Agarose Fine resolution 20 bp 800 bp 0 200 bp 400 bp 600 bp 800 bp 1,000 bp 25,000 bp 10,000,000 bp High gel strength GTG Performance certified Genetic Technology Grade Low melting temperature 11

BioResearch Nucleic Acid Electrophoresis SeaKem Agarose The gold standard for routine DNA/RNA analysis. Routine separation and blotting of large fragments Wide resolution range and easy to dissolve SeaKem LE Agarose supports broad band separation of 100 bp 23 kb fragments, and optimized for PCR fragments with <1 kb SeaKem GTG Agarose SeaKem LE formulation with Genetic Technology Grade (GTG ) Performance certification SeaKem Gold Agarose supports separation of DNA 50 kb 10 Mb by pulsed field methods, and rapid separation of DNA 30 50 kb by horizontal methods and is GTG Performance certified. SeaPlaque Agarose The original low-melting temperature agarose. Optimized for recovery and cloning for 200 bp 25 kb Ideal for cloning tissue culture cells and viral plaque assays SeaPlaque Agarose Low melting temperature agarose designed for recovery and in-gel reactions for large fragments SeaPlaque GTG Agarose GTG Performance certified for large fragment recovery and in-gel reactions NuSieve Agarose Reliable choice for PCR and RT-PCR analysis. Exceptional resolution for small fragments between for 50 bp 1 kb High gel strength for blotting NuSieve 3:1 Agarose Finely resolves DNA fragments < 1,000 bp NuSieve GTG Agarose NuSieve 3:1 formulation with GTG Grade performance certification MetaPhor Agarose The highest resolution agarose available. Ultra-fine resolution, detects 2% size difference from 20 bp 800 bp Ideal for small PCR, RT-PCR and STR analysis Specialty Agarose Lonza also offers a range of agaroses for special applications including: SeaPrep Agarose Ultra-soft agarose ideal for high efficiency hybridoma cloning and expansion of cdna libraries I.D.NA Agarose DNA identity testing for VNTRs, HVRs, RFLPs, and DNA sizing standards InCert Agarose Low-gelling temperature GTG Certified agarose, certified for use in the preparation and digestion of chromosomal DNA prior to the pulsed field gel electrophoresis (PFGE) ß-Agarase An enzyme that will digest the polysaccharide backbone of molten agarose into alcohol soluble oligosaccharides 12

Ordering Information Agarose Cat. No. Description Size SeaKem LE Agarose 50001 SeaKem LE Agarose 25 g 50002 SeaKem LE Agarose 100 g 50000 SeaKem LE Agarose 125 g 50004 SeaKem LE Agarose 500 g 50005 SeaKem LE Agarose 1 kg SeaKem GTG Agarose 50071 SeaKem GTG Agarose 25 g 50070 SeaKem GTG Agarose 125 g 50074 SeaKem GTG Agarose 500 g SeaKem Gold Agarose 50152 SeaKem Gold Agarose 25 g 50150 SeaKem Gold Agarose 125 g SeaPlaque Agarose 50101 SeaPlaque Agarose 25 g 50100 SeaPlaque Agarose 125 g SeaPlaque GTG Agarose 50111 SeaPlaque GTG Agarose 25 g 50110 SeaPlaque GTG Agarose 125 g MetaPhor Agarose 50181 MetaPhor Agarose 25 g 50180 MetaPhor Agarose 125 g 50184 MetaPhor Agarose 250 g NuSieve Agarose 50091 NuSieve Agarose 25 g 50090 NuSieve Agarose 125 g 50094 NuSieve Agarose 500 g NuSieve GTG Agarose 50081 NuSieve GTG Agarose 25 g 50080 NuSieve GTG Agarose 125 g 50084 NuSieve GTG Agarose 500 g SeaPrep Agarose 50302 SeaPrep Agarose 25 g I.D.NA Agarose 50170 I.D.NA Agarose 25 g InCert Agarose and Megabase Standards 50121 InCert Agarose 1 g 50123 InCert Agarose 5 g 50401 Lambda DNA ladder 50 kb to 873 kb 50411 Saccharomyces cerevisiae DNA Standard 220 kb to approximately 1 Mb chromosomal DNA ß-Agarase 58005 ß-Agarase 500 units Larger package sizes are available upon request. Please inquire for pricing and availability. 13 5 plugs (10 ± 2 µg DNA/plug) 5 plugs (10 ± 2 µg DNA/plug) 58001 ß-Agarase 100 units

BioResearch Nucleic Acid Electrophoresis Ladders and Markers for DNA and RNA Optimal Performance Offers Assurance in Your Results Good performance starts with high quality agarose and precast gels, followed by the same high standards for markers, ladders and stains. Lonza offers a wide range to help assure your results. Clear sharp band patterns on each gel using quality markers and ladders Visualization of bands with quality stains 500 bp 200 bp 1,000 bp 500 bp 1,000 bp 700 bp 525 bp 500 bp 400 bp 300 bp 2,500 bp 1,500 bp 1,000 bp 700 bp 525 bp 500 bp 400 bp 1,000 bp 500 bp 3,000 bp 200 bp 200 bp 300 bp 1,000 bp 100 bp 200 bp 100 bp 100 bp 100 bp 100 bp 500 bp 50 bp 50 bp 20 bp 20 bp 100 bp 20 bp 20 bp Ext. 50 1,000 bp 50 2,500 bp 100 bp 100 bp Ext. Standard DNA Ladders 50330 50320 50461 50631 50321 50322 Cat. No. SimplyLoad DNA Ladders 50331 50326 n/a n/a 50327 50328 Fragment sizing made easy: Standard Ladders, size range 20 bp 8,000 bp Standard Quantitation Ladders, size range 100 bp 1,000 bp Standard DNA Ladders, size range 50 bp 10 kb SimplyLoad DNA Ladders, no need for mixing, heating or diluting prior to loading RNA Maker, size range 0.5 kb 9 kb 14

Ordering Information Standard Ladders and Markers Cat. No. Description Size Range Applications Standard Ladders 50330 20 bp DNA Ladder 150 µl 20 bp 500 bp 100 50320 20 bp Extended Range DNA Ladder 150 µl 20 bp 1,000 bp 100 50321 100 bp DNA Ladder Concentration: 100 ng/µl 160 µl 100 bp 1,000 bp 100 50322 100 bp Extended Range DNA Ladder 150 µl 100 bp 3,000 bp 100 50323 500 bp DNA Ladder 300 µl 500 bp 8,000 bp 200 Standard Quantitation Ladders 50334 DNA QuantLadder 125 µl 100 bp 1,000 bp 50 50335 DNA Reverse QuantLadder 125 µl 100 bp 1,000 bp 50 Standard DNA Ladders 50461 DNA Marker 50 1,000 bp 250 µl 50 bp 1,000 bp 50 50471 DNA Marker 1 10 kb 2 250 µl 1 kb 10 kb 100 50631 DNA Marker 50 2,500 bp 250 µl 50 bp 2,500 bp 50 Ordering Information SimplyLoad Ladders Cat. No. Description Size Range Applications SimplyLoad DNA Ladders 50331 SimplyLoad 20 bp DNA Ladder 500 µl 20 bp 500 bp 100 50326 SimplyLoad 20 bp Extended Range DNA Ladder 500 µl 20 bp 1,000 bp 100 50327 SimplyLoad 100 bp DNA Ladder 500 µl 100 bp 1,000 bp 100 50328 SimplyLoad 100 bp Extended Range DNA Ladder 500 µl 100 bp 3,000 bp 100 50329 SimplyLoad 500 bp DNA Ladder 500 µl 500 bp 8,000 bp 100 50333 SimplyLoad Tandem DNA Ladder 500 µl 100 bp 12,000 bp 100 SimplyLoad Quantitation Ladders 50336 SimplyLoad DNA QuantLadder 250 µl 100 bp 1,000 bp 100 50337 SimplyLoad Reverse DNA QuantLadder 250 µl 100 bp 1,000 bp 100 Ordering Information RNA Markers Cat. No. Description Size 50575 FlashGel RNA Marker 0.5 9 kb 50 µg (1 µg/µl) 15

BioResearch Nucleic Acid Electrophoresis Stains Exquisitely Sensitive In-gel and Fluorescent Stains for DNA and RNA Maximum sensitivity compared to silver staining methods Complete staining solution for all types of nucleic acids GelStar Nucleic Acid Gel Stain detects 20 pg of dsdna or 3 ng of RNA with a simple stain added prior to gel casting, or post stain, no de-staining required SYBR Green I Nucleic Acid Gel Stain detects 60 pg dsdna and 1 ng oligonucleotide, best for PCR Stain and Method ssdna dsdna GelStar Stain in gel 25 pg 20 pg Ethidium bromide, no destain 1.25 ng 350 pg Ethidium bromide, no destain 350 pg 100 pg SYBR Green I or II Stain 60 pg 20 30 pg SYBR Green II Nucleic Acid Gel Stain detects 100 pg of ssdna and 2 ng RNA, best for RNA SYBR Green Gel Stain Photographic Filter required for optimal sensitivity with black and white film Ordering Information Nucleic Acid Gel Stains Cat. No. Description Size GelStar Nucleic Acid Gel Stain 50535 GelStar Nucleic Acid Gel Stain 2 250 µl 50536 GelStar Gel Stain Photographic Filter (Wratten #9) 3 inch square SYBR Green Nucleic Acid Gel Stain 50513 SYBR Green I Nucleic Acid Gel Stain supplied as a 10,000X concentrated solution in DMSO. 50512 SYBR Green I Nucleic Acid Gel Stain supplied as a 10,000X concentrated solution in DMSO. 50523 SYBR Green II Nucleic Acid Gel Stain supplied as a 10,000X concentrated solution in DMSO. 50522 SYBR Green II Nucleic Acid Gel Stain supplied as a 10,000X concentrated solution in DMSO. 50530 SYBR Green Gel Stain Photographic Filter (Wratten #15) 10 50 µl 2 500 µl 10 50 µl 2 500 µl 3 inch square 16

AccuGENE Buffers Formulated for Maximum Performance for a Wide Range of Applications Manufactured to strict quality control standards Lot-to-lot consistency Ready-to-use solutions to reduce preparation time Guaranteed DNase, RNase and protease free Customized solutions are available upon request Ordering Information AccuGENE Buffers Cat. No. Description Size AccuGENE Buffers 51200 AccuGENE Molecular Biology Water 1 L 51244 AccuGENE Molecular Biology Water 4 L 51223 AccuGENE Molecular Biology Water 10 L 51224 AccuGENE Molecular Biology Water 20 L 51201 AccuGENE 0.5 M EDTA Solution Disodium salt, ph 8.0 100 ml 51234 AccuGENE 0.5 M EDTA Solution Disodium salt, ph 8.0 1 L 51202 AccuGENE 5 M Sodium Chloride 1 L 51206 AccuGENE 10% SDS Monosodium salt 100 ml 51213 AccuGENE 10% SDS Monosodium salt 500 ml 51203 AccuGENE 3 M Sodium Acetate ph 5.2 500 ml 51205 AccuGENE 20X SSC Buffer 3.0 M NaCl, 0.3 M sodium citrate, ph 7.0 1 L 51233 AccuGENE 20X SSC Buffer 3.0 M NaCl, 0.3 M sodium citrate, ph 7.0 10 L 51232 AccuGENE 20X SSC Buffer 3.0 M NaCl, 0.3 M sodium citrate, ph 7.0 20 L 51214 AccuGENE 20X SSPE Buffer 3.0 M NaCl, 0.2 M NaH 2 PO 4, H 2 O, 0.02 M EDTA, ph 7.4 1 L 51246 AccuGENE 20X SSC Buffer 3.0 M NaCl, 0.3 M sodium citrate, ph 7.0 10 L 51235 AccuGENE 1X TE Buffer 0.01 M Tris, 0.001 M EDTA (disodium salt), ph 7.4 500 ml 51242 AccuGENE 1X TE Buffer 0.01 M Tris, 0.001 M EDTA (disodium salt), ph 7.4 10 L 51236 AccuGENE 1 M Tris HCl Buffer ph 7.2 1 L 51237 AccuGENE 1 M Tris HCl Buffer ph 7.4 1 L 51238 AccuGENE 1 M Tris HCl Buffer ph 8.0 1 L 51217 AccuGENE LB Broth (Luria Bertani Medium) 10 g/l Bacto-Tryptone, 5 g/l Bacto-Yeast Extract, and 10 g/l NaCl 500 ml 51219 AccuGENE Super Broth (Terrific Broth) 12 g/l Bacto-Tryptone, 24 g/l Bacto-Yeast Extract, 6.3 g/l glycerol, 2.5 g/l K 2 HPO 4, and 3.8 g/l KH 2 PO 4, ph 7.2 51221 AccuGENE Super Broth (Terrific Broth) 12 g/l Bacto-Tryptone, 24 g/l Bacto-Yeast Extract, 6.3 g/l glycerol, 2.5 g/l K 2 HPO 4, and 3.8 g/l K 2 HPO 4, ph 7.2 51229 AccuGENE Neutralization Solution 1.5 M NaCl, 1.0 M Tris, ph 7.5 1 L 51230 AccuGENE Neutralization Solution 1.5 M NaCl, 1.0 M Tris, ph 7.5 10 L 51225 AccuGENE 1X PBS 1.7 mm K 2 HPO 4, 5 mm Na 2 HPO 4, 150 mm NaCl, ph 7.4 1 L 51226 AccuGENE 10X PBS 0.017 M K 2 HPO 4, 0.05 M Na 2 HPO 4, 1.5 M NaCl, ph 7.4 1 L 500 ml 1 L 17

BioResearch Nucleic Acid Electrophoresis Ordering Information AccuGENE Buffers Cat. No. Description Size Buffers for DNA Electrophoresis 50844 AccuGENE 10X TAE Buffer 0.4 M Tris-acetate, 0.01 M EDTA (disodium salt), ph 8.0 1 l 50841 AccuGENE 10X TAE Buffer 0.4 M Tris-acetate, 0.01 M EDTA (disodium salt), ph 8.0 4 l 51216 AccuGENE 50X TAE Buffer 2.0 M Tris-acetate, 0.05 M EDTA, ph 8.3 1 l 50839 AccuGENE 5X TBE Buffer 0.45 M Tris-borate, 0.01 M EDTA (disodium salt), ph 8.3 4 l 50835 AccuGENE 5X TBE Buffer 0.45 M Tris-borate, 0.01 M EDTA (disodium salt), ph 8.3 10 l 50836 AccuGENE 5X TBE Buffer 0.45 M Tris-borate, 0.01 M EDTA (disodium salt), ph 8.3 20 l 50843 AccuGENE 10X TBE Buffer 0.89 M Tris-borate, 0.02 M EDTA (disodium salt), ph 8.3 1 l 50840 AccuGENE 10X TBE Buffer 0.89 M Tris-borate, 0.02 M EDTA (disodium salt), ph 8.3 4 l 50837 AccuGENE 10X TBE Buffer 0.89 M Tris-borate, 0.02 M EDTA (disodium salt), ph 8.3 10 l 50838 AccuGENE 10X TBE Buffer 0.89 M Tris-borate, 0.02 M EDTA (disodium salt), ph 8.3 20 l Buffers for RNA Electrophoresis 50876 AccuGENE 10X MOPS Buffer 0.2 M MOPS (free acid), 0.05 M sodium acetate, 0.01 M EDTA (disodium salt), 0.01 M EGTA (free acid), ph 7.0. No detectable RNase activity 1 l Electrophoresis Loading Buffers 50655 DNA Loading Buffer (6X) Ficoll based with bromophenol blue and xylene cyanol 5 1 ml 50632 Triple-Dye Loading Buffer (6X) Contains bromophenol blue, xylene cyanol, and orange G 1.1 ml 18

GelBond Film Supports Agarose Gels With a Transparent, Flexible Polyester Film Gels cast on GelBond Film remain permanently attached to the film through electrophoresis or immunodiffusion with all subsequent fixing, staining, detaining and dying procedures Reliable and durable to retain original dimensions with easy handling Gel orientation can be recorded directly on the film prior to casting Ordering Information GelBond Film Cat. No. Description Size (mm) Chamber Compatibility GelBond Film Sheets and Rolls 53734 GelBond Film Sheets for agarose gels; 0.2 mm thick, 100 sheets 85 100 53745 110 125 53746 100 150 Bio-Rad Wide Mini-Sub Cell, Bio-Rad Sub-Cell (H) 53748 110 205 53749 160 180 Hoefer SE400, SE600 (V) Bio-Rad PROTEAN II xi (V) 53759 125 245 53761 124 258 GE Multiphor (H) 53740 GelBond Film Rolls for agarose gels; 0.2 mm thick rolls 53750 127 53780 203 102 Custom-cut GelBond Film is available upon special request. Please inquire for pricing and availability. (H) = Horizontal; (V) = Vertical 19

www.westburg.eu Contact Information North America Scientific Support: +1 800 521 0390 (toll free) scientific.support@lonza.com Europe Scientific Support: +32 87 321 611 scientific.support.eu@lonza.com Lonza Walkersville, Inc. Walkersville, MD 21793 For research use only. Not for use in diagnostic procedures. Some components and technology of the FlashGel System are sold under licensing agreements. The nucleic acid stain in this product is manufactured and sold under license from Molecular Probes, Inc., and the FlashGel Cassette is sold under license from Invitrogen IP Holdings, Inc, and is for use only in research applications or quality control, and is covered by pending and issued patents. The FlashGel Dock technology contains Clare Chemical Research, Inc. Dark Reader transilluminator technology and is covered under US Patents 6,198,107; 6,512,236; and 6,914,250. The electrophoresis technology is licensed from Temple University and is covered under US Patent 6,905,585. Bio-Rad Sub-Cell and Bio-Rad Wide Mini-Sub Cell are registered trademarks of of Bio-Rad Laboratories, Inc. GelBond and SeaKem are registered trademarks of FMC Corp. GelStar is a trademark of FMC Corp. Ficoll and GE Multiphor is a registered trademark of GE Healthcare Biosciences AB Ltd. Hoefer is a registered trademark of Hoefer Scientific Instruments. SYBR is a registered trademark of Molecular Probes Corporation, Inc. Wratten is a registered trademark of Eastman Kodak Company Corp. All other trademarks herein are marks of the Lonza Group Ltd or its affiliates. The information contained herein is believed to be correct and corresponds to the latest state of scientific and technical knowledge. However, no warranty is made, either expressed or implied, regarding its accuracy or theresults to be obtained from the use of such information and no warranty is expressed or implied concerning the use of these products. The buyer assumes all risks of use and/or handling. Any user must make his own determination and satisfy himself that the products supplied by Lonza Group Ltd or its affiliates and the information and recommendations given by Lonza Group Ltd or its affiliates are (i) suitable for intended process or purpose, (ii) in compliance with environmental, health and safety regulations, and (iii) will not infringe any third party s intellectual property rights. To order: NL: 033 494 6666 BE/LUX: 0800 19815 orders@westburg.eu Technical Support: techsupport@westburg.eu Copyright 2014, Lonza Walkersville, Inc. All rights reserved. MB-BR008 01/14