Reproductive Management in Beef Herds. Marcos G. Colazo Dairy Research Scientist Alberta Agriculture & Rural Development Edmonton, Alberta, Canada

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Reproductive Management in Beef Herds Marcos G. Colazo Dairy Research Scientist Alberta Agriculture & Rural Development Edmonton, Alberta, Canada

Reproduction is the single most important factor affecting profitability!

PROFITABILITY OF BEEF PRODUCTION Reproduction is 5X more important than Growth Rate. Reproduction is 10X more important than Carcass Quality.

CAUSES OF DECREASED PROFITABILITY 1.Percentage of non-pregnant cows 2.Late born calves 3.Loss of calves at birth

CALVING DISTRIBUTION

CALVING DISTRIBUTION AND PROFIT Survey of 2,713 cow-calf producers in north-central Alberta. Decreasing length of calving season was best gross opportunity for increasing profit ($23/head). Economic impact of changing calving distribution from 29 to 49% calving in the 1 st 21 days was $ 39/head. Basarab, 1987

Time of calving affects feedlot performance Period of calving (21 days) Steers (n=661) 1 2 3 Weaning weight, kg 234 220 198 Feedlot ADG, kg/día 1.6 1.6 1.6 Carcass weight, kg 371 364 350 Marbling score 574 554 527 Yield grade 3.0 2.8 2.6 Choice, % 84 83 73 Carcass value, USD $1632 $1600 $1542

Time of calving affects heifer progeny Period of calving (21 days) Heifers (n=1019) 1 2 3 Weaning weight, kg 220 214 197 Prebreding weight, kg 297 293 277 Cycling, % 70 58 38 Breeding ADG, kg 0.70 0.74 0.77 Pregnancy rate, % 90 86 78 Calved in first 21 d, % 81 69 65

Non-pregnant cows (%) Effect of calving date on the number of cows calving the following year 30 25 26.1 20 17.9 15 10 6.9 9.4 12.9 5 0 1 2 3 4 5 Period of calving (21 days) Paterson et al., 1992

Calving Interval Calving Conception Calving Gestation Uterine Involution Optimum period 55 days Gestation 280 days D-30 D-85 Breeding season 85 days Interval between calvings 12 months

ANESTROUS CONDITION NUTRIENT INTAKE PREPARTUM NUTRIENT INTAKE POSPARTUM SUCKLING

BODY CONDITION SCORE BCS CAN BE USED TO PREDICT BODY FATS STORES 9-POINT SCALE 1= EMACIATED; 9 = OBESE 36-45 Kg BW equals one condition score

BODY CONDITION SCORE 4

BODY CONDITION SCORE 5

BODY CONDITION SCORE 6

BODY CONDITION SCORE 7

EFFECT OF BCS ON OVERALL PREGNANCY RATE (90 DAYS BREEDING SEASON) Selk et al., 1988

BCS ON ANESTROUS INTERVAL (LATE WINTER/EARLY SPRING CALVING) BCS <5 5 Reference 61 ± 2 49 ± 2 Richards et al., 1986 89 ± 6 53 ± 4 Looper et al., 2002 93 ± 6 63 ± 6 Lents et al., 2002 81 ± 4 55 ± 4 DIFF 26 days

Percent of beef cows in heat at different intervals after calving Days after calving Age of cows 40 50 60 70 80 90 100 5 years or older (%) 55 70 80 90 90 95 100 2 and 3 years (%) 15 30 40 65 80 80 90 Wiltbank et al., 1962

EFFECT OF BCS AT CALVING ON PERFORMANCE OF FIRST CALF COWS BCS CRITERIA 4 5 6 N 73 107 60 Cow BW (Kg) 338 a 375 b 424 c Calf BW 28.9 a 30.4 b 32.4 c Dystocia score 1.2 1.2 1.2 205 d weight, kg 187 193 198 Pregnancy rate 56 a 80 bw 96 bx ab P<0.05 xw P=0.07 Spitzer et al., 1995

INFLUENCE OF POSTPARTUM NUTRITION ON PERFORMANCE OF THIN FIRST CALF COWS Moderate = 0.45 kg/d High = 0.90 kg/d Nutrition treatments CRITERIA Moderate High BCS at calving 4.1 4.4 BCS at breeding 4.3 5.2* Daily gain, kg/d 0.2 1.5* Days to first estrus, d 120 100* Size of ovulatory follicle, mm 13.5 14.8* Pregnancy to first AI 58 76** * P<0.01; ** P<0.05 Ciccioli et al., 2003

Re-breeding youngfirst calf cows is a big challenge!

What can be done? Sort cows: - by BCS (thin or moderate) - by Age (young or mature) - At 6 mo. of pregnancy - At calving Feed them to achieve desired BCS!

EFFECT OF 48 HOUR CALF REMOVAL ON COWS SHOWING ESTRUS AND CONCEIVING First 21 days of breeding No. of cows Bred Pregnant CONTROL 52 31% 17% CALF REMOVAL 52 62% 44% Smith et al., 1979

INFLUENCE OF EARLY WEANING ON BW AND REPRODUCTIVE PERFORMANCE OF THIN FIRST CALF COWS 24-30 mo.; weaned 6-8 weeks and exposed to bulls for 90 days TREATMENTS ITEMS Suckled Early weaned No. 32 31 BW at calving, kg 316 ± 7 309 ± 5 BW change, kg (calving-breeding) -7 ± 10 15 ± 14* Calving to conception, d 90 ± 3 73 ± 4* Pregnancy rate, % 59 97 * P<0.05 Lusby et al., 1981

AI: One of the beef industry s most under utilized technology

BEEF SEMEN SOLD (units) 1993 2012 Change USA 1.117.798 1.670.000 +66.9% BRASIL 1.874.996 7.440.000 +252% NAAB, 2012; ASBIA, 2012

Reasons for the low adoption of AI in beef herds in USA % 100 90 80 70 60 50 40 30 20 10 0 Beef Dairy 4 80 % 40 35 30 25 20 15 10 5 0 Labour & time Difficult to apply Cost Facility Doesn t work NAHMS 2007

Cost of Natural service (Bull/4 years) Purchase $3,000 $4,000 $5,000 $6,000 Slaughter value -$1,500 -$1,500 -$1,500 -$1,500 Difference $1,500 $2,500 $3,500 $4,500 Maintenance/year $1,000 $1,000 $1,000 $1,000 Vet bill/year $100 $100 $100 $100 Death (10%/year) $1,200 $1,600 $2,000 $2,400 Total Cost $7,100 $8,500 $9,900 $11,300 No. calves 112 112 112 112 Cost per calf $63.39 $75.89 $88.40 $100.89

Timed-AI programs: Cost per pregnancy based on 100 heifers CIDR/EB CIDR/OVS CIDR/COS7 CIDR/COS5 CIDR/COS5M Drugs $ 26.5 $ 30.0 $ 30.0 $ 35.0 $ 26.5 Labour $ 300 $ 300 $ 240 $ 300 $ 240 Semen & AI $ 2500 $ 2500 $ 2500 $ 2500 $ 2500 P/AI 65% 55% 50% 65% 60% Cost per pregnancy $ 83.8 $ 105.5 $ 114.8 $ 96.9 $ 89.8

TAI vs. Natural service: Percentage of animals conceiving in the first week of the breeding season

TAI vs. Natural service (Study 1) Groups Difference TAI Natural service Cows 251 109 --- Calving, % 90 81 9 Calved first 30 days, % 85 62 23 Weaning,% 88 79 9 Average age at weaning, days 210 200 10 Weaning weight, kg 262 230 32 Weaning weight of exposed cow, kg 231 181 50 Anderson y Deaton, 2003

300 250 200 150 Weaning weight of male calves (adjusted 240 days) 215 MACHOS (PESO AJUSTADO AOS 240 DIAS) 8 223 26 241 100 50 0 216 208 NELORE TOURO NELORE IA ANGUS IA Responsible técnico: Luciano Penteado; cortesia del Dr. Baruselli, USP, Brasil

Factors that affect fertility in an AI program 1. Nutrition (BCS) 2. Record: Age, postpartum interval 3. Anestrous 4. Compliance 5. Weather

AVERAGE DAILY GAINS (KG/DAY) OF HEIFERS DEVELOPED IN RANGE OR DRY-LOT AFTER MOVED TO A PASTURE Salverson et al., 2009

REPRODUCTIVE PERFORMANCE OF HEIFERS DEVELOPED IN RANGE OR DRY-LOT AFTER MOVED TO A PASTURE Perry et al., 2009

REPRODUCTIVE PERFORMANCE OF HEIFERS DEVELOPED IN RANGE OR DRY-LOT AFTER MOVED TO A PASTURE Perry et al., 2009

EFFECT OF PROTEIN SUPPLEMENTATION DURING POSTPARTUM Lens et al., 2008

ESTRUS SYNCRONIZATION PROTOCOLS

Commercially available PGF products

Follicle size (mm) Active CL, D 5-16 20 Growing CL PGF effective Regressing CL 15 10 Ov Ov 5 0 7 14 21 Day of cycle Metestrus Diestrus Pro estrus Estrus

Common Protocols using PGF Estrus detection and AI Estrus detection and AI EDR~80% 6 days PGF Estrus detection and AI EDR~85% PGF 11 days PGF Estrus detection and AI PGF 14 days PGF EDR~85%

% in heat Distribution of heat in Holstein heifers given PGF 14 d apart n = 132 60 50 40 30 20 10 0 36 48 60 72 84 96 108 120 132 144 Hours after second PGF Total in heat: 86% 45% ovulated 30% don t ovulate 25% don t respond Colazo, unpublished

Conception Rate % P r e g n a n t 100 90 80 70 60 50 40 30 20 10 0 a b 75% 65% Conventional semen Sexed semen a, b Effect of TRT P < 0.05

Oral Progestins: Melengestrol Acetate (MGA) Ear implants : Crestar Intravaginal Devices PRID CIDR-B DIB TRIU-B CUE-MATE

Oral Progestins: Melengestrol Acetate (MGA) Ear implants : Crestar Intravaginal Devices PRID CIDR-B DIB TRIU-B CUE-MATE

Program with MGA for estrus synchronization MGA Day 0 14 (17/19 days) NO AI PGF 31/33 ED + AI MGA = melengestrol acetate @ 0.5 mg/heifer/day

Modified MGA program (MGA select) MGA Day 0 14 GnRH 26 PGF 33 ED + AI NO AI Wood-Follis et al., 2004

MGA/PGF vs. MGA Select (ED + AI for 6 days) Anestrus Cycling 100 90 80 70 60 50 40 30 20 10 0 80 93 80 96 MGA/PGF MGA select 90 80 70 60 50 40 30 20 10 0 * * 66 67 63 82 MGA/PGF MGA select Estrus rate Pregnancy rate N = 124 Wood-Follis et al., 2004

PROTOCOLS FOR TIMED-AI

TAI protocol (Progesterone & Estrogen) DAY 0 7 8 9 Insert CIDR Give 1 or mg EB o 5 mg E-17B Remove CIDR Give PGF 1 mg EB TAI

TAI protocol-schedule (Progesterone & Estrogen) MON am TUE am WED pm THU FRI SAT SUN EB or 17B CIDRi CIDRr EB TAI PGF 28-34 hours

Pregnancy rate in heifers treated with E17B or EB % 100 90 80 70 60 50 40 30 20 10 0 65 E17B 56 Cyclic Acyclic 59 BE 46 n = 1428 heifers Colazo et al., 2001; 2002; 2004; 2005

Some commercially available GnRH products Fertiline (Vetoquinol) Cystorelin (Merial) Factrel (Pfizer Animal Health) Fertagyl (Merck Animal Health)

How Ovsynch works? D 0 D 7 D 9 D 10 GnRH PGF GnRH AI 7 d 2 d 16 h Induced ovulation New follicle growth CL regression and Follicle maturation Synchronized Ovulation Pursley et al., 1995

Cosynch Second GnRH given at time of AI D 9 GnRH D 0 D 7 D 9 GnRH PGF + AI AI 7 d 2 d 0-12 h Advantage: Reduced handling Possible disadvantage: Delayed ovulation

Cosynch plus a P4 device D 0 D 7 D 9 GnRH PGF GnRH&AI P4 Device 56-60 h In D 0 Out D 7

TAI protocol-schedule (7d Cosynch & CIDR) MON am TUE am WED pm THU FRI SAT SUN GnRH CIDRi CIDRr PGF GnRH & TAI 56-60 hours 400 IU ecg

Experimental Design - 5 d vs 7 d Cosynch GnRH PGF 2α 7 d CIDR 60 h d -7 d -5 d 0 d 0 GnRH + AI 5 d CIDR 72 h GnRH PGF 2α Day et al., 2006

TAI Pregnancy Rate % P r e g n a n t 100 90 80 70 60 50 40 30 20 10 0 a b CO-Synch 5d CIDR CO-Synch 7d CIDR 120/201 100/204 a, b Effect of TRT P < 0.03

TAI protocol-schedule (5d Cosynch & PRID) MON am TUE am WED am THU am FRI SAT SUN GnRH PRIDi PRIDr PGF PGF GnRH & TAI

No. of calvings Calving distribution of 90 beef heifers all bred on a single day 14 12 10 8 6 4 2 0 270 272 274 276 278 280 282 284 286 Gestation length (days) N = 90 Reuter and Rinette, 2009

First FTAI 50-60 % Half of the cows are still open!! What are the possibilities? Bulls for 45 to 60 days -Estrus detection & AI of the returns -ReSynchronization & AI -ReSynchronization & TAI

Days Resynchronization 0 (TAI) 13 20 26 CIDR Estrus detection Control Total n=159 Vacias n=69

Number of heifers bred and pregnant by treatment 14 12 10 8 6 4 2 0 9 8 7 6 5 4 3 2 1 0 CIDR Control 70.6% 85.7% 15 16 17 18 19 20 21 22 23 26 Days after TAI EDR Varianza P=0.07

Pregnancy rates (%) Conception rate 80 60 40 44.1 65.7 20 0 CIDR (n=34) Control (n=35) P=0.07

Resyncronization with an used CIDR or MGA Groups Control CIDR-B MGA Preg. to 1 st AI (%) 45.2 43.4 49.5 42 121 138 Estrus rate (%) 48.5 ab 53.4 a 41.2 b n 45/51 149/158 115/141 Conception rate (%) 62.2 ab 65.1 a 49.6 b n 28/45 97/149 57/115 Pregnancy rate (%) 54.9 ab 61.4 a 40.4 b n 28/51 97/158 57/141 Accumulated PR 60/93 218/279 195/279 (%) 64.5 x 78.1 y 69.9 x abc Percentages are different (P<0.05); xy P<0.003

Re-TAI Program for Beef Cattle TAI GnRH US PGF GNRH TAI Re-used P4 device D 0 D 16 D 21 D 28 D30 D30 AM PM Courtesy of Dr. Bo

Commercial TAI Programs using Re-TAI (2007/2011) N 1º TAI % 2º TAI % Total % Heifers 4267 56% 51% 77% Cows 415 65% 47% 84% Suckled Cows 1749 58% 51% 80% TOTAL 6431 57% 51% 78% Courtesy of Dr. Bo

CONCLUSIONS 1. AI in commercial beef herds: Improve genetics of the herd Improve progeny performance Allow for the incorporation of new breeds 2. The use of TAI: Reduce the breeding and calving season Increase the proportion of cows calving in the first 21 days Increase the average weaning weight of calves Induce cyclicity in anestrous and prepubertal cattle

Thank You! Marcos G Colazo Research Scientist, Alberta Agriculture & Rural Development Edmonton, Alberta, Canada E-mail: marcos.colazo@gov.ab.ca; mgcolazo@yahoo.com