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1(a)(i) A description including two of the following: (2) as ph decreases so do the of species in the lake accept: the more acidic the lake is the lower the of species {more species / types of organisms} live in more neutral conditions /less organisms live where {low ph /more acidic} all the organisms /species in the lake are found at 6.0/6.5 comment on specific reading from the graph e.g. (only) {frogs / 1 organism} remain at lowest ph. eq. 1(a)(ii) D sulfur dioxide

1(b) An explanation linking three of the following: (3) eutrophication causes an algal bloom plants {at the bottom of the lake / underneath the algae} cannot get light to photosynthesise plants at the bottom of the lake die and microorganisms break them down accept: increased growth of algae accept: decomposers for microorganisms microorganisms respire removing oxygen from the water reduction in biodiversity Ignore references to fish suffocating / dying this is insufficient for this marking point

Answer Q1(c) condition indicator species clean water stonefly (larvae / nymph)/ (freshwater) shrimp / mayfly larvae/nymph/ caddis fly larvae / dragonfly nymph / damselfly nymph / riffle beetle / water penny larvae / polluted water bloodworm / sludgeworm / algae / rat tailed maggot / water louse/ leeches/ planarian worms / aquatic worms / Mark Ignore general references to organisms such as frogs / snails/ squid the answers need to be specific indicator species. If unsure of an organism then please put into review Total for 1 = 8 marks

2(a) A description to include three of the following (3) selection of individuals with {favourable characteristics/largest cobs}/collect seeds from plants with large cobs cross breeding (of selected individuals)/plant seeds together (from maize with large cobs) accept selective breeding selection of offspring repeat process over time 2(b) An explanation including two of the following (2) reduce of pests accept kill pests/insects/named pest/deters animals reject predators reduced damage to crop/maize increased yield OR kills weeds reduces competition for {light/space/named resource} increased yield

2(c) A discussion to include a maximum of two from Advantages: (4) removes CO 2 when growing accept carbon neutral reject CO 2 less use of {fossil fuels/named fuel} reduced SO 2 emissions reject SO 2 renewable / can be regrown quickly /sustainable A discussion to include a maximum of two from Disadvantages: reduced food production accept deforestation takes up land reduced biodiversity crop growth is weather dependent ignore references to cost and energy content 2(d) A Agrobacterium tumefaciens Total for 2 = 10 marks

3(a)(i) photosynthesis 3(a)(ii) A description of the processes that return carbon dioxide to the atmosphere including respiration in animals / respiration from arrow 2 respiration in plants / respiration from arrow 5 decomposition /respiration by microorganisms / decomposition /respiration arrow 3 accept trees combusting/burning releasing CO 2 ignore - references to arrow 1 returning carbon dioxide to the atmosphere / photosynthesis / references to arrow 4 (3) 3(b) An explanation linking the first bullet point with an explanation including increase in carbon dioxide levels Plus one of the following respiration/ burning of fossil fuels/ waste decaying maximum 1 mark for reason accept named fossil fuel deforestation leading to reduced photosynthesis (2)

3 (c) lichen / blackspot fungus other air quality indicator species eg. canaries / algae / moss / peppered moths 3(d) An explanation linking three of the following including points algal bloom/ increased algae / more algae Ignore encourages algae to grow blocks sunlight (from plants growing on the bottom of the lake/river) so stops photosynthesis (plants die) so decomposers break them down Accept bacteria/microorganisms for decomposers No mark for plants die which use oxygen for respiration /oxygen depletion Do not give mark for just low oxygen this must be linked to microorganisms (respiring) (3) (Total for question 3 = 10 marks)

4(a) C least amount of freshwater shrimps found at C Reference to freshwater shrimps as indicator species freshwater shrimps can only survive in clean water / cannot survive in polluted water more shrimps die in polluted water (2) 4(b) D 4(c) C 4(d) A description of the process linking four of the following points: algae (on the surface) of the stream show rapid growth (they) block light to the photosynthesising plants below algal bloom occurs / large increase in growth of algae /other plants grow quickly (causing) plants on the stream bed to die decomposers use up oxygen to break down these dead plants other organisms die due to lack of oxygen Accept microorganisms / microbes / bacteria Accept reference to anaerobic bacteria can function in anoxic conditions - not against a current marking point (4) (Total for question 4 = 8 marks)

Answer Additional guidance Mark 5(a)(i) 2.1 x 10 4 = 21 000 x 0.1 = 2 100 in the water beetle 210 J in the bird award full marks for correct numerical answer without working (2) 5(a)(ii) Answer it limits the length of the food chain Mark Answer Additional guidance Mark 5(b)(i) 107 153 0.699 3464 100 = 70% Answer to 2 significant figures award full marks for correct numerical answer without working (2) 5(b)(ii) Answer Additional guidance Mark An explanation that combines identification via a judgment (1 mark) to reach a conclusion via justification/reasoning (1 mark): stream B is more polluted that stream A Plus one from: (because) stream A contains stonefly larvae/mayfly larvae/caddis fly larvae (which are indicators of clean water) (because) stream B contains larger s of blood worm and sludge worm (which are indicators of polluted water) accept other correct indicators from the table. accept higher oxygen levels in place of clean water accept lower oxygen levels in place of polluted water (2) 5(c) Answer Mark An explanation that combines identification understanding (1 mark) and reasoning/justification understanding (3 marks): plants growing on the bottom of the stream will be unable to receive sunlight due to the thick layer of algae these plants will not be able to photosynthesise and will die and start to decompose the microorganisms decomposing the plants will respire, removing oxygen from the water the stream will become anoxic/oxygen depleted and other respiring organisms (plants and animals) will not be able to survive so biodiversity will be reduced (4) (Total for question 5 = 11 marks)