Protein Synthesis Review Bi 12 /25

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Name: Class: _ Date: _ Protein Synthesis Review Bi 12 /25 Multiple Choice Identify the choice that best completes the statement or answers the question. 1. A certain gene codes for a polypeptide that is 120 amino acids long. Approximately how many nucleotides long is the mrna that codes for this polypeptide likely to be? a. 30 c. 360 b. 40 d. 480 2. Where do transcription and translation occur in eukaryotic cells? Transcription Translation A. Cytoplasm Cytoplasm B. Cytoplasm Mitochondria C. Nucleus Cytoplasm D. Nucleus Nucleus a. Cytoplasm Cytoplasm c. Nucleus Cytoplasm b. Cytoplasm Mitochondria d. Nucleus Nucleus 3. Where is an anti-codon located? a. trna c. DNA b. mrna d. Ribosomes Ver 1.1 Oct 2017 Bi 12 1

Name: 4. Which stage of translation is illustrated by the diagram below? a. Termination c. Translocation b. Initiation d. Elongation 5. A certain gene in a bacterium codes for a polypeptide that is 120 amino acids long. How many nucleotides are needed in the mrna to code for this polypeptide? a. 30 c. 360 b. 40 d. 480 6. What enzyme is used in transcription but not in translation? a. DNA polymerase c. Protease b. Helicase d. RNA polymerase 7. If mrna has a codon CAU, what is the corresponding anticodon on the trna molecule? a. CAT c. CAU b. GUA d. GTA Ver 1.1 Oct 2017 Bi 12 2

Name: 8. CCU - Proline GGU - Glycine GAC - Arginine CAG - Glutamine GGA - Tyrosine CUG - Leucine GUC - Valine GUC - Valine AAA - Lycine AGC - Serine UUG - Cysteine CCA - Proline Use the above list of codons for amino acids to answer the following question. Reading from left to right, which one of the following is the first amino acid to be positioned in building a protein using the DNA strand GGTCAGCCG? a. Valine. c. Proline. b. Glycine. d. Tyrosine. 9. What is a change in the base sequence in a strand of DNA called? a. Feedback inhibition. c. Mutation. b. Enzyme repression. d. Positive feedback. 10. The molecule in the above diagram is a. DNA c. polypeptide b. RNA d. polysaccharide 11. The unit molecule or monomer ( see X in the diagram above ) is a/an a. monosaccharide c. amino acid b. nucleotide d. fatty acid 12. The process of producing a mrna molecule from a strand of DNA is called a. translation. c. DNA replication. b. transcription. d. a chromosome mutation. 13. What is the minimum number of bases required to code for one amino acid? a. 1 b. 2 c. 3 d. 4 Ver 1.1 Oct 2017 Bi 12 3

Name: 14. Protein synthesis occurs at the a. mitochondria. c. Golgi body. b. centriole d. ribosomes. 15. Use the following events to answer question 6. 1. mrna is formed 2. DNA segment opens (unzips) 3. mrna attaches to ribosomes 4. amino acids form peptide bonds 5. trna carries amino acids to mrna The correct order of events required for protein synthesis is a. 1, 2, 3, 4, 5. c. 2,1, 3, 5, 4. d. 2, 1, 4, 5, 3. b. 2, 1, 4, 5, 3. Short Answer 16. What type of bond holds paired nucleotides together? (1 mark) 17. (a) State two differences between the structure of DNA and RNA. (2 marks) 1....... 2....... Ver 1.1 Oct 2017 Bi 12 4

Name: The diagram below shows the structure of a ribosome during protein synthesis. 18. State the names of the structures labelled above. (4 marks) I.... II.... III.... IV.... 19. Describe the process of DNA replication. (3 marks) Ver 1.1 Oct 2017 Bi 12 5

Protein Synthesis Review Bi 12 /25 Answer Section MULTIPLE CHOICE 1. ANS: C PTS: 1 REF: IB 2nd Bank 2. ANS: C PTS: 1 REF: IB 2nd Bank 3. ANS: A PTS: 1 REF: IB 2nd Bank 4. ANS: D PTS: 1 REF: IB 2nd Bank 5. ANS: C PTS: 1 REF: IB 2nd Bank 6. ANS: D PTS: 1 REF: IB 2nd Bank 7. ANS: B PTS: 1 REF: IB 2nd Bank 8. ANS: C PTS: 1 9. ANS: C PTS: 1 10. ANS: B PTS: 1 11. ANS: B PTS: 1 12. ANS: B PTS: 1 13. ANS: C PTS: 1 14. ANS: D PTS: 1 15. ANS: C PTS: 1 SHORT ANSWER 16. ANS: Hydrogen bonds PTS: 1 17. ANS: Answers must either give DNA characteristic first or specify which is DNA and which is RNA. deoxyribose versus ribose; thymine versus uracil; two strands versus one / double helix versus single strand; 2 max PTS: 2 Ver 1.1 Oct 2017 Bi 12 1

18. ANS: IV: Award [2] for four correct and [1] for three or two correct. I. small (sub)unit (of ribosome); II: large (sub)unit (of ribosome); III: transfer RNA / trna; messenger RNA / mrna; PTS: 2 19. ANS: Helicase unzips DNA strand Complementary nucleotides bond DNA polymerase bond them together Ligase cleans up bond PTS: 1 Ver 1.1 Oct 2017 Bi 12 2