BIOLOGY 230 LAB SCHEDULE FALL 2017 DATE Current Lab Activity Report, Paper & Test Schedule WEDNESDAY AUGUST 23 Introductory Comments BIOMOLECULES & BIOCHEMISTRY Build Molecules Monosaccharides Nitrogen Bases Fatty Acids: 2 sat s, 1 unsat d Assignments: Not all assignments are graded. Graded assignments are specifically marked. See the syllabus for the value of each graded assignment Due Dates & Tests INTRODUCTION TO THE MICROSCOPE August 30 BIOMOLECULES & BIOCHEMISTRY September 6 DIFFUSION and OSMOSIS September 13 ENZYME GRADED DUE September 20! Enzyme Lab Questions! Use the Template on the Lab Page of Website under ENZYME LAB heading 17Aug17 1
September 20 Enzyme Lab Questions Introduction to Biotechnology Series PART 1 Introduction to Biotechnology Equipment Enzyme Lab Questions Homework DUE BRING AN EXTRA COPY TO USE DURING CLASS SUBMIT THE ORIGINAL KEEP THE COPY September 27 Construction & Cloning of a Recombinant DNA Scientific Method & Protocol Analysis MODULE 1 - Cloning kan r fragment GRADED DUE October 4! Transformation Flowchart (use Module 1 Flowchart as a model) October 4 MODULE 1 - Run confirming gel on Pre- & Post-Ligation samples MODULE 2 - Transformation Transformation Flow Chart Homework DUE BRING AN EXTRA COPY TO USE DURING Thursday October 5 Counting Putative Transformants Follow Directions in Lab Protocol 17Aug17 2
October 11 FIRST PART OF LAB LAB TEST #1 MODULE 3- Scale-Up of Putative Transformants MODULE 2 - Transformation (con t) Collect Transformation Data Transformation Efficiencies Purpose of Controls LAB TEST #1 S Intro. to Microscope S Biomolecules & Biochemistry S Diffusion & Osmosis S Enzyme Activity S Biotechnology Series Vocabulary October 18!Review Lab Test #1!Review Exam #1 (if completed) PART 3 AN INTRODUCTION TO COMPUTER-BASED BIOINFORMATICS Biotechnology Series Part 4: DNA Fingerprinting Using RFLP Read and complete the problem at the end of the exercise. Purchase SimUText materials Read Outline Section 8a You may begin the SimuText material at home Lab Time for Simutext completion in lab on 11/1 & 11/19. 17Aug17 3
October 25 LONG DAY COME PREPARED MODULE 4 - Plasmid Extraction Discuss Restriction Map Homework during 15' pellet drying step GRADED DUE November 1! Restriction Map Worksheet MENDELIAN GENETICS USING BRASSICA RAPA Plant F2 seeds (score in 2 weeks) Genetics - Part 1! Complete all exercise and problems in the Genetics Worksheet located in the appendix of the Lab Manual! Read Mendelian Genetics using Brassica Rapa Lab Exercise in Lab Manual Complete Exercises in Part 2 - Expected Results COMPLETE BEFORE 11/9 LAB 17Aug17 4
November 1 MODULE 5a - Plasmid Restriction Restriction Map Homework Expected Gel Results Worksheet Completing and submitting Biotech Lab Report Part 1 BEGIN OR CONTINUE Section 8: The Cell Cycle Section 8a: Mitosis & Meiosis - BRING SIMUTEXT Log-In Information Section 8b: Mendelian Genetics Restriction Map Worksheet DUE BRING AN EXTRA COPY TO USE DURING CLASS Graded Lab Report Assignment: Biotech Lab Report - Part 1 Construction and Cloning of a DNA Molecule DUE November 17! Complete Biotech Lab Report Part 1 using the temple on the Lab Report Download Page on the Course Website! This report will be submitted as a hard copy.! Carefully follow the instructions at the of the Lab Report Download page Genetics - Part 2 Read in Essential Cell Biology textbook! Chapter 18: 621-633! Chapter 19:! Complete the following problems: 18-17 (skip Item E) 19-6 19-7 19-8 19-10, 19-12(A&B)(Pedigree problem) 19-15 COMPLETE ALL GENETICS HOMEWORK (Parts 1&2) BEFORE LAB ON November 8 17Aug17 5
November 8 MODULE 5b - Electrophoresis & Analysis Complete THE CELL CYCLE Lab Activity in the Lab Manual using the Online Resources available on the LAB section of the course website. MENDELIAN GENETICS WITH BRASSICA RAPA Mitosis and Meiosis micrographs will be on Lab Test #2 Collect P, F1, & F2 data Enter Data in Spreadsheet Perform Chi-Square Test on Class and Sample Data Complete by Nov. 22 Completing and submitting Biotech Lab Report Part 2 WILL BE ASSIGNED NEXT WEEK BEGIN OR CONTINUE Section 8: The Cell Cycle Section 8a: Mitosis & Meiosis BRING YOUR SIMUTEXT MATERIALS Section 8b: Mendelian Genetics 17Aug17 6
November 15 PART 5 Using the Polymerase Chain Reaction (PCR) to Type Human DNA SNPs, RFLP and PCR Collect and Extract DNA Set-Up and Start PCR Reaction GRADED LAB REPORT DUE Biotech Lab Report - Part 1 Construction and Cloning of a DNA Molecule Submit as a Hard-Copy in Lab. Be sure to complete all sections before coming to class Reports MUST BE SUBMITTED BEFORE THE END OF LAB LATE REPORTS WILL BE PENALIZED UP TO 50% Graded Lab Report Assignment: Biotech Lab Report - Part 2 DNA Fingerprinting and PCR DUE NOVEMBER 22! Complete Biotech Lab Report Part 2 using the template on the Lab Report Download Page on the Course Website! This report will be submitted as a hard copy.! Carefully follow the instructions at the top of the Lab Report Download page Reminder - Chi-Square Data assignment for November 22 17Aug17 7
November 22 November 23 November 29 PART 5 Using the Polymerase Chair Reaction (PCR) to Type Human DNA Process samples using Gel Electrophoresis & Discuss results PHOTOSYNTHESIS Determine %Absorption for Grass Pigments Review Graph of Data MENDELIAN GENETICS WITH BRASSICA RAPA COLLECT Chi-Square Homework Discuss Chi-Square Test on F2 and Summary Data CELLULAR RESPIRATION Extract Mitochondrial Enzymes Test for Cytochrome Oxidase Activity Discuss Results Discuss Submitting Report via Blackboard GRADED LAB REPORT DUE Biotech Lab Report - Part 2 DNA Fingerprinting & PCR Submit as a Hard-Copy in Lab. Be sure to complete all sections before coming to class Reports MUST BE SUBMITTED BEFORE THE END OF LAB LATE REPORTS WILL BE PENALIZED UP TO 50% Graded Lab Report Assignment: Photosynthesis & Cell Respiration DUE MONDAY DECEMBER 4! Complete Photosynthesis & Cell Respiration Report using the template on the Blackboard! This report will be submitted electronically via Blackboard! Be sure to follow the instructions at the top of the Lab Report Download page MONDAY December 4 Lab Report #3 - Photosynthesis & Cell Respiration DUE VIA BLACKBOARD by 11pm, Monday DECEMBER 4 LATE REPORTS WILL NOT BE ACCEPTED 17Aug17 8
December 6 LAB TEST #2 - Cell Cycle Stages - Mitosis & Meiosis including Simbio Materials - Biotechnology Series - Genetics - Photosynthesis & Cellular Respiration 17Aug17 9