SECTION 1: PRODUCT AND COMPANY IDENTIFICATION PRODUCT NAME: EZClick Global Protein Synthesis Assay Kit (FACS/Microscopy), Red Fluorescence PRODUCT CODES: Cat# K715-100 MANUFACTURER: BioVision, Inc. DIVISION: ADDRESS: 155 S. Milpitas Blvd. Milpitas, CA 95035 EMERGENCY PHONE: 858-373-8066 CHEMTREC PHONE: OTHER CALLS: 408-493-1800 FAX PHONE: 408-493-1801 SECTION 2: COMPOSITION/INFORMATION ON INGREDIENTS Component Description Volume Safety Information EZClick TM Wash Buffer (10X) Liquid 25 ml No hazards Fixative Solution Solution (contains Formaldehyde) 10 ml See below Permeabilization Buffer (10X) Liquid 25 ml No hazards EZClick TM Protein Label (400X) Liquid 25 µl No hazards Copper Reagent (100X) Solution (contains Copper Reagent) 100 µl See below EZClick TM Fluorescent Azide (100X) Liquid 100 µl No hazards Reducing Agent (20X) Liquid 500 µl No hazards EZClickTM Total DNA Stain (1000X) In DMSO 10 µl See below Cycloheximide (100X) In DMSO 10 µl See below Copper Reagent: Emergency Overview GHS Classification: Acute aquatic toxicity (Category 1), H400 Chronic aquatic toxicity (Category 1), H410 GHS Label elements, including precautionary statements Pictogram: Signal word: Warning Hazard statement(s): H410 Very toxic to aquatic life with long lasting effects. Precautionary statement(s): P273 Avoid release to the environment. P391 Collect spillage. P501 Dispose of contents/ container to an approved waste disposal plant. HMIS Classification Health hazard: 2 Fire hazard: * Flammability: 0 Physical hazards: 0 NFPA Rating Health Hazard: 2 Fire: 0 Reactivity Hazard: 0 Inhalation: Toxic if inhaled. Causes respiratory tract irritation. Skin: Toxic if absorbed through skin. Causes skin irritation. Eyes: Causes eye irritation. Emergency Overview PAGE 1 OF 7
OSHA Hazards: Target organ effect, Toxic by ingestion, Toxic by skin absorption, Skin and respiratory sensitizer, Corrosive, Carcinogen Target Organs: Eyes, Kidney, Liver, Heart GHS Classification: Acute toxicity, Oral (Category 3) Acute toxicity, Dermal (Category 3) Serious eye damage (Category 1) Skin irritation (Category 2) Respiratory sensitization (Category 1) Skin sensitization (Category 1) Carcinogenicity (Category 2) Acute aquatic toxicity (Category 3) GHS Label elements, including precautionary statements Pictogram: Signal word: Hazard statement(s): Precautionary statement(s): Danger H301+H311 Toxic if swallowed or in contact with skin. H315 Causes skin irritation. H317 May cause an allergic skin reaction. H318 Causes serious eye damage. H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled. H351 Suspected of causing cancer. H402 Harmful to aquatic life. P261 Avoid breathing dust/fumes/gas/mist/vapors/spray. P264 Wash hands thoroughly after handling. P273 Avoid release to the environment. P280 Wear protective gloves/protective clothing/eye protection/face protection. P301+P310 IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician. P305+P351+P338 IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P311 Call a POISON CENTER or doctor/physician. HMIS Classification Health hazard: 3 Chronic health hazard: * Flammability: 0 Physical hazards: 0 NFPA Rating Health Hazard: 3 Fire: 0 Reactivity Hazard: 0 Inhalation: May be harmful if inhaled. Material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract. Skin: Toxic if absorbed through skin. Causes skin burns. Eyes: Causes eye burns. Emergency Overview OSHA Hazards: Combustible liquid, Target organ effect Target Organs: Eyes, Skin GHS Classification: Flammable liquids (Category 4) GHS Label elements, including precautionary statements Pictogram: none Signal word: Warning Hazard statement(s): H227 Combustible liquid Precautionary statement(s): none HMIS Classification Health hazard: 0 Chronic Health Hazard: * Flammability: 2 Physical hazards: 0 NFPA Rating Health hazard: 0 Fire: 2 Reactivity Hazard: 0 Inhalation: May be harmful if inhaled. May cause respiratory tract irritation. Skin: May be harmful if absorbed through skin. May cause skin irritation. Eyes: May cause eye irritation. Ingestion: May be harmful if swallowed. PAGE 2 OF 7
Aggravated Medical Condition: Avoid contact w/dmso solutions containing toxic materials or materials with unknown toxicological properties. DMSO is readily absorbed through skin and may carry such materials into the body. SECTION 3: HAZARDS IDENTIFICATION Component CAS Number EC-No. Molecular Weight Chemical Formula Copper Reagent -- -- -- -- Formaldehyde 50-00-0 200-001-8 30.03 HCHO DMSO 67-68-5 200-664-3 78.13 C2H6OS SECTION 4: FIRST AID MEASURES General advice: Consult a physician. Show this safety data sheet to the doctor in attendance. Move out of dangerous area. If inhaled: If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician. In case of skin contact: Wash off with soap and plenty of water. Consult a physician. In case of eye contact: Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician. If swallowed: DO NOT induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician. SECTION 5: FIRE-FIGHTING MEASURES Suitable extinguishing media: For small (incipient) fires, use media such as "alcohol" foam, dry chemical, or carbon dioxide. For large fires, apply water from as far as possible. Use very large quantities (flooding) of water applied as a mist or spray; solid streams of water may be ineffective. Cool all affected containers with flooding quantities of water. Special protective equipment for firefighters: Wear self-contained breathing apparatus for firefighting if necessary. Hazardous combustion products: Hazardous combustion products formed under fire conditions no data available. Further information: Use water spray to cool unopened containers. SECTION 6: ACCIDENTAL RELEASE MEASURES Personal precautions: Use personal protective equipment. Avoid dust formation. Avoid breathing vapors, mist, or gas. Ensure adequate ventilation. Evacuate personnel to safe areas. Avoid breathing dust. Environmental precautions: Prevent further leakage or spillage if safe to do so. Do not let product enter drains. Discharge into the environment must be avoided. Methods for cleaning up: Pick up and arrange disposal without creating dust. Sweep up and shovel. Keep in suitable, closed containers for disposal. SECTION 7: HANDLING AND STORAGE Precautions for safe handling Avoid contact with skin and eyes. Avoid inhalation of vapor or mist. Use explosion-proof equipment. Keep away from sources of ignition (no smoking). Take measures to prevent the buildup of electrostatic charge. Conditions for safe storage Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. Recommended storage temperature: -20 C SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION Components CAS-No. Value Control parameters Basis Formaldehyde 50-00-0 C 0.3 ppm USA. ACGIH Threshold Limit Values (TLV) Eye & upper respiratory tract irritation. Suspected human carcinogen. Sensitizer Remarks: Substance listed, for more information see OSHA document 1910.1048 See 1910.1048 TWA 0.016 ppm USA. NIOSH Recommended Exposure Limits Potential occupational carcinogen. See Appendix A, 15 minutes ceiling value. C 0.1 ppm USA. NIOSH Recommended Exposure Limits Potential occupational carcinogen. See Appendix A, 15 minutes ceiling value. Components CAS-No. Value Control parameters Basis Dimethyl sulfoxide 67-68-5 TWA 250 ppm USA. Workplace Environmental Exposure Levels (WEEL) Personal protective equipment Respiratory protection PAGE 3 OF 7
Where risk assessment shows air-purifying respirators are appropriate use a full-face respirator with multi-purpose combination (US) or type ABEK (EN 14387) respirator cartridges as a backup to engineering controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU). Hand protection Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands. Eye protection Face shield and safety glasses. Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN 166(EU). Skin and body protection Complete suit protecting against chemicals. The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace. Hygiene measures Handle in accordance with good industrial hygiene and safety practice. Wash hands before breaks and at the end of workday. SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES Property Copper Reagent Formaldehyde DMSO Appearance: blue liquid Clear liquid Clear liquid ph: No data available No data available No data available Water Solubility: No data available No data available Completely miscible Other Solubility: No data available No data available No data available Boiling Point ( C): No data available No data available 189 o C (372 o F) Melting Point ( C): No data available No data available 16-19 o C (61-66 o F) Flash Point ( C): No data available No data available 87 o C (189 o F) Ignition Temperature ( C): No data available No data available 301 o C (574 o F) Density: No data available No data available 1.1 g/ml SECTION 10: STABILITY AND REACTIVITY Property Copper Reagent Formaldehyde DMSO Chemical stability: Stable under recommended storage conditions Conditions to avoid: No data available No data available Heat, Flames, Sparks Powdered metals, Anhydrous Acid chlorides, Phosphorus Materials to avoid: copper(ii) sulfate, reacts halides, Strong acids, Strong Strong oxidizing agents violently with:, oxidizing agents, Strong hydroxylamine, Magnesium reducing agents Hazardous decomposition products: No data available Carbon oxides Carbon oxides, sulfur oxides SECTION 11: TOXICOLOGICAL INFORMATION Copper Reagent: Acute toxicity: no data available Skin corrosion/irritation: no data available Serious eye damage/eye irritation: no data available Respiratory or skin sensitization: no data available Germ cell mutagenicity: no data available Carcinogenicity: IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. ACGIH: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by ACGIH. NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated OSHA: carcinogen by NTP. No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by OSHA. Reproductive toxicity: no data available Teratogenicity: no data available Specific target organ toxicity single exposure (GHS): Causes damage to organs. Specific target organ toxicity repeated exposure (GHS): no data available Inhalation: Toxic if inhaled. Causes respiratory tract irritation. Skin: Toxic if absorbed through skin. Causes skin irritation. Eyes: Causes eye irritation. PAGE 4 OF 7
Signs and Symptoms of Exposure: no data available Additional information: no data available RTECS: no data available Acute toxicity: no data available Skin corrosion/irritation: no data available Serious eye damage/eye irritation: Eyes: no data available Respiratory or skin sensitization: no data available Germ cell mutagenicity: no data available Carcinogenicity: IARC: 1 - Group 1: Carcinogenic to humans (Formaldehyde) NTP: Reasonably anticipated to be a human carcinogen (Formaldehyde) Reproductive toxicity: no data available Teratogenicity: no data available Specific target organ toxicity - single exposure (Globally Harmonized System): no data available Specific target organ toxicity - repeated exposure (Globally Harmonized System): no data available Aspiration hazard: no data available Inhalation: May be harmful if inhaled. Material is extremely destructive to the tissue of the mucous membranes and upper respiratory tract. Skin: Toxic if absorbed through skin. Causes skin burns. Eyes: Causes eye burns. Signs and Symptoms of Exposure: Exposure may cause burning sensation, cough, wheezing, laryngitis, shortness of breath, spasm, inflammation and edema of the larynx, spasm, inflammation and edema of the bronchi, pneumonitis, pulmonary edema. Material is extremely destructive to tissue of the mucous membranes and upper respiratory tract, eyes, and skin. Acute toxicity: LD50 Oral rat 14,500 mg/kg LC50 Inhalation rat 4 h 40250 ppm LD50 Dermal rabbit >5,000 mg/kg Skin corrosion/irritation: Skin rabbit no skin irritation 4h Serious eye damage/eye irritation: Eyes rabbit mild eye irritation Respiratory or skin sensitization: no data available Germ cell mutagenicity: Genotoxicity in vitro mouse lymphocyte: Cytogenetic analysis Genotoxicity in vitro mouse lymphocyte: Mutation in mammalian somatic cells Genotoxicity in vivo rat Intraperitoneal: Cytogenetic analysis Genotoxicity in vivo mouse Intraperitoneal: DNA damage Carcinogenicity: Carcinogenicity rat Oral Tumorigenic: equivocal tumorigenic agent by RTECS criteria. Skin and appendages: other: tumors. Carcinogenicity mouse Oral Tumorigenic: equivocal tumorigenic agent by RTECS criteria. Leukemia skin and appendages: other: tumors. IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. ACGIH: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by ACGIH. NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated OSHA: carcinogen by NTP. No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by OSHA. Reproductive toxicity: Reproductive toxicity rat Intraperitoneal Effects on fertility: abortion Reproductive toxicity rat Intraperitoneal Effects on fertility: post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants) Reproductive toxicity rat Subcutaneous Effects on fertility: post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants). Effects on fertility: litter size (e.g. # fetuses per litter; measured before birth) Reproductive toxicity mouse Oral Effects on fertility: post-implantation mortality (e.g. reduction in number of implants per female; total number of implants per corpora lutea). Effects on embryo/fetus: Fetotoxicity (except death, e.g. stunted fetus). Specific developmental abnormalities: musculoskeletal system. Teratogenicity: Developmental toxicity mouse Intraperitoneal: Effects on embryo/fetus: Fetotoxicity (except death, e.g. stunted fetus). Specific developmental abnormalities: musculoskeletal system Specific target organ toxicity single exposure (GHS): no data available Specific target organ toxicity repeated exposure (GHS): no data available Aspiration hazard: no data available Inhalation: May be harmful if inhaled. May cause respiratory tract irritation. Skin: May be harmful if absorbed through skin. May cause skin irritation. Eyes: May cause eye irritation. Ingestion: May be harmful if swallowed. Aggravated Medical Condition: Avoid contact w/dmso solutions containing toxic materials or materials with unknown toxicological properties. DMSO is readily absorbed through skin and may carry such materials into the body. Signs and Symptoms of Exposure: Effects due to ingestion may include: nausea, fatigue, and/or headache. PAGE 5 OF 7
Additional information: RTECS: PV6210000 SECTION 12: ECOLOGICAL INFORMATION Persistence and degradability: no data available Toxicity: no data available Bioaccumulative potential: no data available Mobility in soil: no data available PBT and vpvb assessment: no data available Other adverse effects: no data available SECTION 13: DISPOSAL CONSIDERATIONS Product: Observe all federal, state, and local environmental regulations. Contaminated packaging: Dispose of as unused product. SECTION 14: TRANSPORT INFORMATION Copper Reagent: DOT (US): UN-number: 3082, Class: 9, Packing group: III; Proper shipping name: Copper Reagent; Reportable Quantity (RQ): 400 lbs.; Marine pollutant: No; Poison inhalation hazard: No IMDG: UN-number: 3082, Class: 9, Packing group: III; EMS-No: F-E, S-D; Proper shipping name: COPPER REAGENT; Marine pollutant: No IATA: UN-number: 3082, Class: 9, Packing group: III; Proper shipping name: Copper Reagent DOT (US): UN number: 2209, Class: 8, Packing group: III; Proper shipping name: Formaldehyde solutions; Reportable Quantity (RQ): 200 lbs; Marine pollutant: No; Poison Inhalation Hazard: No IMDG: UN number: 2209, Class: 8, Packing group: III; EMS-No: F-A, S-B; Proper shipping name: FORMALDEHYDE SOLUTION; Marine pollutant: No IATA: UN number: 2209, Class: 8, Packing group: III; Proper shipping name: Formaldehyde solution DOT (US): UN-Number: 1993 Class: CBL Packing group: III; Proper shipping name: Combustible liquid, n.o.s. (Dimethyl sulfoxide); Marine pollutant: No; Poison Inhalation Hazard: No IMDG: Not dangerous goods. IATA: Not dangerous goods. SECTION 15: REGULATORY INFORMATION SARA 302 Components: SARA 302: The following components are subject to reporting levels established by SARA Title III, Section 302 SARA 313 Components: The following components are subject to reporting levels established by SARA Title II, Section 313: SARA 311/312 Hazards: Copper Reagent: Acute Health Hazard, Chronic Health Hazard Acute Health Hazard, Chronic Health Hazard Dimethyl sulfoxide: Fire Hazard, Chronic Health Hazard Massachusetts Right To Know Components: Pennsylvania Right To Know Components: Dimethyl sulfoxide CAS-No. 67-68-5; Revision Date: 2007-03-01 New Jersey Right To Know Components: Dimethyl sulfoxide CAS-No. 67-68-5; Revision Date: 2007-03-01 California Prop. 65 Components: This product does not contain any chemicals known to State of California to cause cancer, birth defects, or any other reproductive harm. EU regulations: Component Risk Phrases Safety Phrases Copper Reagent R11, R23/24/25, R33 S16, S24/25, S36/37, S45 Formaldehyde R24/25, R35, R40, R42/43 S26, S36/37/39, S45, S51 DMSO R10, R36/37/38 S24/25, S36/37/39, S45 PAGE 6 OF 7
SECTION 16: OTHER INFORMATION: DISCLAIMER: The above information is believed to be correct but does not purport to be all inclusive and shall be used only as a guide. The information in this document is based on the present state of our knowledge and is applicable to the product with regard to appropriate safety precautions. It does not represent any guarantee of the properties of the product. BioVision, Inc., shall not be held liable for any damage resulting from handling or from contact with the above product. See reverse side of invoice or packing slip for additional terms and conditions of sale. PAGE 7 OF 7