A Top Tip System! H I G H - T E C H D I S P O S A B L E S

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A Top Tip System! Pipette Tips and Filter Tips PLASTIBRAND universal tips and filter tips are a proven standard worldwide. They are optimized to adapt to specific volumes. The polypropylene used is free from DiHEMDA and oleamide, two frequently used additives that affect biological tests and can result in spurious results. The new 200 μl and 1000 μl tips are now 25% lighter, which significantly reduces the weight of plastic waste. Graduations for a quick volume check BIO-CERT : sterile, free of DNA, DNase, RNase, endotoxins and ATP Autoclavable at 121 C Environmentally friendly packaging H I G H - T E C H D I S P O S A B L E S 100 µl

2 µl PLASTIBRAND Pipette Tips Graduation 0. 1 - nano- cap 37 mm long, specially developed for the nanoliter range. The tip features a capillary rise that is even visible to the naked eye at 0.1 µl. Racked in a grey-colored tip tray. 50 - New, lower-weight universal tips! 70 mm long, particularly thin-walled and transparent. Graduations at 250, 500 and for a quick volume check. Bulk tips are blue colored, racked tips are colorless in blue-colored tip tray. 50-1 82 mm long, ideal for pipetting from narrow vessels and for reverse pipetting. Graduations at 250, 500 and for quick volume check. Racked in orange-colored tip tray. 10 µl 0. 5-46 mm long. The slender design allows pipetting in microtubes and microplates without touching the walls. The tip is graduated at 2 µl and 10 µl for quick volume check. Racked in a grey-colored tip tray. 2 µl 100 µl 2-200 µl New, lower-weight universal tips! 50 mm long, particularly thin-walled and transparent. Graduations at and 100 µl for a quick volume check. Bulk tips are yellow colored, racked tips are colorless in yellow-colored tip tray. 0. 5-5 ml 160 mm long and approx. 9.6 mm diameter, particularly slender shape. Pipetting even from narrow volume measuring equipment such as volumetric flasks with NS 12/21. Suitable for microliter pipette Transferpette, Transferpette S and Thermo Fisher Scientific Finnpipette. 5-300 µl (without graduation) 52 mm long, suitable for reverse pipetting. The tip can also be used for pipettes with yellow color code. Racked in green-colored tip tray. 1-10 ml 156.5 mm long and approx. 15 mm diameter! Ideal for working with the microliter pipette Transferpette S 10 ml. Compatible with pipettes from Eppendorf and Gilson. Request your samples at www.brand.de!

PLASTIBRAND Filter Tips non- self- sealing Filter tips are especially suited for working with PCR techniques, and fully meet the requirements for microbiology and radioisotope work. The integrated hydrophobic polyethylene filter serves as a barrier against aerosols formed during pipetting. Filter tips protect the pipette shafts from contamination, and thus lessen the risk of cross-contamination and faulty measuring results. PLASTIBRAND filter tips are produced from colorless granules under the most stringent cleanroom conditions and are automatically racked. Autoclavable at 121 C (20 min). Standard Tip Filter Tip 0.1-1 µl (0.1 - tip nano-cap ) 0.5-10 µl (0.5 - tip) 2 - (2-200 µl tip) 10 µl 2 µl 5-100 µl (5-300 µl tip without graduation) 5-200 µl (5-300 µl tip without graduation) 50 - (50-1 tip) The advantages of non-self-sealing filter tips Non-self-sealing filter tips have a PE filter that is free from chemical additives. Permeability is controlled by the combination of pore size and filter length, so that no aerosols can reach the pipette shaft. These filters function with consistent reliability. On the other hand, liquids can pass very slowly should they accidentally contact the filter. Since the filter does not swell, the sample can be recovered from the filter by simply actuating the pipette's blow-out function, or by centrifugation if necessary. This is clearly an important advantage of nonself-sealing filters, especially when working with valuable samples. As an added advantage, the absence of filter additives protects samples from this source of contamination. The disadvantages of self-sealing filter tips Self-sealing filter tips have a PE filter that swells through possible additives on contact with liquids. This causes development of a barrier that should not let any liquid pass. Since this behavior is only initiated by diffusing liquids, a considerable part of the sample is already in the filter and cannot be recovered. This is clearly a disadvantage with expensive, laboriously produced samples. In addition, additives in the polyethylene filter can contaminate the sample.

Package Types in bulk In bags Tips and filter tips are produced under the latest state-of-the-art clean room conditions and automatically shrink-wrapped in bags and packaged in cardboard boxes. For easy identification, the catalog number, volume range and lot number of the tips are found on every bag. racked, non-sterile Tip-Box N Made of PP. With push-on lid. Stackable and autoclavable at 121 C (t e 20 min). Two different heights: low form (up to 300 µl): rack with 96 tips high form ( or 1): rack with 100 tips Tip-Box SL Made of PC. Innovative box with slide & swing lid. For convenient work with single channel and multichannel pipettes. Keeps unused tips covered. Not stackable. Autoclavable at 121 C (t e 20 min). Tip-Box PC Made of PC. This sturdy box has a sliding lid, can be stacked, and is autoclavable at 121 C (t e 20 min). Tip-Box 5 / 10 ml Made of PP. With push-on lid. Filled with 5 ml tips (28 tips) or 10 ml tips (18 tips). The box is autoclavable at 121 C (t e 20 min). Tip-trays used (racks) in Tip-Box N, SL, PC 96-tip rack (up to 300 µl) Tip-Rack Refill units for Tip-Box N, Tip-Box SL or Tip-Box PC. Two filled tip-trays (racks) are packed into a space-saving carton. Up to 300 µl: racks with 96 tips each : racks with 60 or 100 tips each 1: racks with 100 tips each Tip-Stack Space-saving, environmentally compatible refill system for Tip-Box N, Tip-Box SL or Tip-Box PC. Stacked racks with Tip-Box N included. and 200 µl: racks with 96 tips each : racks with 100 tips each Tip-Set For getting started easily. Combination of 4 loaded tiptrays (2 Tip-Racks) and 1 filled Tip-Box PC (for 1000 μl and 1250 μl 1 filled Tip-Box N). 60-tip rack () in Tip-Box N, high form 100-tip rack (, 1)

racked, sterile Tip-Box N sterile Made of PP. With push-on lid. Stackable and autoclavable at 121 C (t e 20 min). Boxes individually packed in bag with perforation. Two different heights: low form (up to 300 µl): rack with 96 tips high form ( or 1): rack with 100 tips Tip-Rack S With 1 loaded BIO-CERT refill unit for the autoclaved Tip-Box N, Tip-Box SL or Tip-Box PC. Protected in an environmentally compatible packaging of recyclable PET. (Tip-Rack S is only available for volume ranges up to 300 µl.) Tip-Set S For getting started easily. Combination of 4 sterile, loaded tip-trays and 1 sterile, filled Tip-Box N. (Tip-Set S is only available for volume ranges up to 300 µl.) PP granules Sensitive applications, such as enzyme tests, PCR or DNA/RNA purification, require plastic disposable products of the highest quality. Specially selected transparent PP types, free from the additives di-(2-hydroxyethyl)methyldodecylammonium (DiHEMDA) and 9-octadecenamide (oleamide), are used for manufacturing the pipette tips. Both of these additives are frequently found in PP granules, and can interfere with biological tests, leading to spurious results (see last page 1) for reference ). PLASTIBRAND tips and filter tips are produced strictly under the most modern cleanroom conditions (ISO 14 644-1 Class 8). BIO-CERT products undergo stringent additional testing. What does BIO-CERT products are certified to be: quality really mean? Sterile BIO-CERT products are sterilized according to ISO 11 137 and AAMI guidelines with b-radiation, using a minimum radiation dose of 12.1 kgy. This gives a SAL (sterility assurance level) of 10-6. The sterility meets USP 29 and Ph. Eur. requirements for sterility. Free of endotoxins The endotoxin concentration in BIO-CERT products is tested by a gel-clotting Limulus Amoebocyte Lysate test. The detection limit is 0.01 EU/ ml, providing a documented endotoxin level of less than 1 x 10-12 g/item. BIO-CERT Free of DNA and RNase BIO-CERT products are DNAfree (< 4 x 10-12 g/item) to prevent false positive signals during PCR applications. These products are free of RNases (< 8.6 x 10-15 g/item) to prevent degradation of RNA during purification processes. Free of ATP BIO-CERT products are certified to be free of ATP (ATP concentration < 1 x 10-15 g/ item) and therefore suitable for ATP detection using bioluminescence-systems. BIO-CERT products are supplied with a certificate.

Package Types at a Glance in bulk racked, non-sterile in bags Tip-Box N Tip-Box SL Tip-Rack Quantity Cat. No. Quantity Cat. No. Quantity Cat. No. Quantity Pipette Tips 0.1-2000 2 bags (1000 each) 7025 04 480 7024 08 96 7022 02 960 0.5-2000 2 bags (1000 each) 7025 26 480 10000 7025 65 10 bags (1000 each) 2-200 µl* 1000, yellow (1000 each) 5-300 µl 1000 7125 16 480 10000, yellow 7125 56 10 bags (1000 each) 7025 33 480 10000 7025 68 10 bags (1000 each) 50 - * 1000, blue 2 bags (500 each) 5000, blue 10 bags (500 each) 50-1 1000 2 bags (500 each) Filter Tips 0. 1-1 µl 960 0. 5-10 µl 960 2-960 5-100 µl 960 5-200 µl 960 50-1000 4000 8 bags (500 each) 7125 21 500 7024 11 96 7124 13 96 7024 15 96 7124 14 60 7022 04 960 7122 06 960 7022 08 960 7122 10 960 16 racks (60 each) 7125 61 1000 7025 81 500 7025 84 * Bulk tips are yellow or blue colored, racked tips are colorless in a yellow-colored or blue-colored tip tray 7024 18 1000 7021 11 960 7021 00 960 7121 02 960 7021 04 960 7021 06 960 7021 08 1000 Pipette Tips, special sizes 5 and 10 ml in bulk Quantity Cat. No. 0. 5-5 ml 200 () 7025 95 1000 (5 bags, 200 each) 7026 00 1-10 ml 200 (2 bags, 100 each) 7026 03 1000 (10 bags, 100 each) 7026 04

racked, sterile, quality Tip-Stack Tip-Set Tip-Box N sterile Tip-Rack S Tip-Set S Cat. No. Quantity Cat. No. Quantity Cat. No. Quantity Cat. No. Quantity Cat. No. Quantity Cat. No. 7023 12 480 7023 04 960 7024 40 960 7024 28 480 7024 19 7023 50 960 7124 02 (available end of 2009) 480 7023 45 960 7024 42 960 7024 31 480 7024 21 7123 15 960 7124 05 (available end of 2009) 480 7123 05 960 7124 44 960 7124 33 480 7124 23 7023 53 480 7023 41 960 7024 46 960 7024 35 480 7024 25 7123 20 1000 2 x 7123 22 7124 08 (available end of 2009) 500 7123 13 1000 7124 48 7023 24 500 7023 14 1000 7024 50 7023 54 960 7021 59 960 7021 39 480 7021 19 7023 55 960 7021 60 960 7021 40 480 7021 20 7123 57 960 7121 59 960 7121 42 480 7121 22 7023 59 960 7021 64 960 7021 44 480 7021 24 7023 61 960 7021 66 960 7021 46 480 7021 26 7023 65 1000 7021 68 racked, non-sterile (not available sterile) Quantity Cat. No. 0. 5-5 ml 28 () 7026 05 Tip-Box SL Tip-Box PC Quantity Cat. No. Quantity Cat. No. 1-10 ml 18 () 7026 08 1 Tip-Box SL (empty) 7022 00 1 Tip-Box PC (empty) 7023 00

The right Pipette Tip PLASTIBRAND pipette tips are tested for BRAND pipettes and most of the pipette types from Gilson, Thermo Fisher Scientific Finnpipette, Eppendorf and Biohit. The 5 ml tip is exclusively tested for BRAND pipettes and Thermo Fisher Scientific Finnpipette. The 10 ml tip is suitable for BRAND, Eppendorf and Gilson. Optimum results are achieved in combination with the BRAND microliter pipettes. PLASTIBRAND quality tips seat perfectly for precise analyses. Transferpette S Transferpette Transferpette electronic Transferpette S -8/-12 Transferpette -8/-12 Transferpette -8/-12 electronic Pipette tips/ Filter tips Volume range 1 µl 5 µl 10 µl Single channel pipettes* Nominal volume ** 25 µl 50 µl 100 µl 200 µl 2 ml 5 ml 10 ml 10 µl Multichannel pipettes* Nominal volume 25 µl 50 µl 100 µl 200 µl 300 µl 0.1-0.5-2 - 200 µl 5-300 µl 50-50 - 1 0.5-5 ml 1-10 ml 0.1-1 µl 0.5-10 µl 2-5 - 100 µl 5-200 µl 50-4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 4 = Tip volume less than pipette's nominal volume *) Electronic pipettes are not available in all specified volumes **) Transferpette with yellow color code 9947 92 Printed in Germany 5/0909 1) G. R. McDonald, A. L. Hudson, S. M. J. Dunn, H. You, G. B. Baker, R. M. Whittal, J. W. Martin, A. Jha, D. E. Edmondson, A. Holt (2008). Bioactive Contaminants Leach from Disposable Laboratory Plasticware. Science, 322 (5903), 917-917. BRAND, BIO-CERT, PLASTIBRAND and Transferpette are trademarks of BRAND GMBH + CO KG, Germany. Other reproduced brands are the property of the respective owner. Our technical literature is intended to inform and advise our customers. However, the validity of general empirical values, and of results obtained under test conditions, for specific applications depends on many factors beyond our control. Please appreciate, therefore, that no claims can be derived from our advice. The user is responsible for checking the appropriateness of the product for any particular application. Subject to technical modification without notice. Errors excepted. BRAND GMBH + CO KG P.O. Box 11 55 97861 Wertheim Germany Tel.: +49 9342 808-0 Fax: +49 9342 808-236 E-Mail: info@brand.de Internet: www.brand.de