Khairnar et al, IJPSR, 2014; Vol. 5(9): 3696-3703. E-ISSN: 0975-8232; P-ISSN: 2320-5148 IJPSR (2014), Vol. 5, Issue 9 (Research Article) Received on 28 February, 2014; received in revised form, 15 April, 2014; accepted, 30 May, 2014; published 01 September, 2014 METHOD DEVELOPMENT AND VALIDATION OF KETOROLAC TROMETHAMINE IN TABLET FORMULATION BY RP-HPLC METHOD Dhiraj A. Khairnar *, Chetan S. Chaudhari and Sanjay P. Anantwar Department of Pharmaceutics, M.V.P. Samaj s College of Pharmacy, Near K.T.H.M. Campus, Gangapur road, Nasik- 422002, Maharashtra, India Keywords: Ketorolac Tromethamine, RP-HPLC method, Method development, Method validation, UV detector Correspondence to Author: Dhiraj Arvind Khairnar Department of Pharmaceutics, M.V.P. Samaj s College of Pharmacy, Near K.T.H.M. Campus, Gangapur road, Nasik- 422002, Maharashtra, India. E-mail:bdhirukhairnar2011@gmail.com ABSTRACT: In present study a simple, precise and accurate method was developed and validated for analysis of Ketorolac Tromethamine in Tablet formulation. A gradient HPLC analysis was performed on Grace C18 column (250 cm 4.6 mm 5µ). The compound was separated with a solvent mixture of Methanol and water in ratio of 65:35 v/v with 0.1% O-phosphoric acid as the mobile phase at flow rate of 1ml/min. UV detection was performed at the ʎ max 245 nm. The retention time was found to be 7.70 min. The system suitability parameters such as theoretical plate count, tailing factor and percentage relative standard deviation (RSD) between six standard injections was within the limit. The method was validated according to International conference of harmonization (ICH) guidelines. The linearity was found to be in the concentration range of 5-25 µgm/ml as indicated by correlation coefficient (r 2 ) of 0.999. The robustness of the method was evaluated by deliberately altering the chromatographic condition. The developed method can be applicable for routine quantitative analysis. INTRODUCTION: Ketorolac tromethamine is a non-steroidal anti-inflammatory drug (NSAID). Chemically 2-Amino-2-(hydroxymethyl) propane- 1, 3-diol (1RS) -5- benzoyl-2, 3-dihydro-1Hpyrrolizine-1-carboxylate (API) (European Pharmacopoeia 2008) as shown in Fig 1. It is a member of the heterocyclic acetic acid derivative family and is used as an analgesic with an efficacy close to that of the opioid family. It is also a potent antipyretic and antiinflammatory. It is mainly used for the short term treatment of post-operative pain as it is highly selective for the COX-1 enzyme. 5 This Analgesic is approved by the USA Food and Drug Administration and it is non-norcotic, fast acting and non-addictive. It can be administered orally or by injection. 12 QUICK RESPONSE CODE DOI: 10.13040/IJPSR.0975-8232.5(9).3696-03 Article can be accessed online on: www.ijpsr.com DOI link: http://dx.doi.org/10.13040/ijpsr.0975-8232.5(9).3696-03 FIG 1: STRUCTURE OF KETOROLAC TROMETHAMINE (MW=376.4) International Journal of Pharmaceutical Sciences and Research 3696
Khairnar et al, IJPSR, 2014; Vol. 5(9): 3696-3703. E-ISSN: 0975-8232; P-ISSN: 2320-5148 Several studies for the estimation of the Ketorolac Tromethamine drug using various techniques have been carried out, some of them being; Development and Validation of Ketorolac Tromethamine in Eye Drop Formulation by RP-HPLC Method 1. RP- HPLC Method Development and Validation of Acuvail Drug 2. New Simultaneous UV-Visible Spectrophotometric Methods for Estimation of Ofloxacin and Ketorolac Tromethamine in Ophthalmic Dosage Form 3. Analytical Method Development and Validation for the Simultaneous Estimation of Febuxostat and Ketorolac in Tablet Dosage Forms by RP-HPLC 4. Development and validation of a rapid liquid chromatographic method for the analysis of Ketorolac Tromethamine and its related production Impurities 5. Simultaneous RP-HPLC Estimation of Moxifloxacin Hydrochloride and Ketorolac Tromethamine in Ophthalmic Dosage Forms 8. New Spectrophotometric Determination of Ketorolac Tromethamine Bulk and Pharmaceutical Dosage Form 9. Two dimensional liquid chromatography-ion trap mass spectrometry for the simultaneous determination of ketorolac enantiomers and paracetamol in human plasma application to a pharmacokinetic study 11. Reversed-phase high performance liquid chromatography of ketorolac and its application to bioequivalence studies in human serum 12. Simple and sensitive method for the analysis of ketorolac in human plasma using high-performance liquid chromatography 13. An indirect (derivatization) and a direct HPLC method for the determination of the enantiomers of ketorolac in plasma 14. EXPERIMENTAL PROCEDURES: Instrument Younglin (S.K 9000) gradient System with UV Detector (Autochro -3000 software), Sartorius Electronic Analytical balance, Crest sonicator and Grace C 18 column (4.6 mm x 250 cm 5µm) was used. Chemical and Reagents Free gift sample of Ketorolac Tromethamine was obtained from FDC limited, Mumbai. A Pharmaceutical product (Ketrol- DT) containing the same amount of drug formulation was used in the experiment HPLC grade methanol was procured from Modern lab, Nasik. HPLC grade deionized water was used throughout the experiment. Mobile Phase Methanol and Water with 0.1% O- Phosphoric acid in ratio of 65:35 v/v used as mobile phase. It was used as diluents for the preparation of sample and standard. METHOD DEVELOPMENT: Wavelength detection Accurately weighed Ketorolac Tromethamine equivalent to 0.1 gm in 100 ml volumetric flask, 100ml methanol was added, sonicate for 5min and filtered through 0.45 nylon membrane filter. Pipette out 1 ml of the above solution and dilute to 10 ml with methanol in 10 mi volumetric flask and scanned between 200-400 nm by UV spectroscopy. The λ max found was 245 nm. Chromatographic conditions Chromatographic separation was achieved at ambient temperature, the detection was carried at 245 nm at a flow rate of 1 ml/min and run time was kept 16 min. Prior to the injection of drug solution column was equilibrated for 60 min with the mobile phase flowing through the system. The injection volume was 20 µl throughout the experiment. Blank containing mobile phase was injected to check the solvent interference. Standard preparation The 10mg of Ketorolac Tromethamine was weighed and transferred into a 10ml volumetric flask and make up to the volume with methanol. From this take 0.05, 0.10, 0.15, 0.20 and 0.25ml and dilute with mobile phase up to 10ml for preparation of 5,10,15,20 and 25 µgm/ml respectively. A representative chromatogram of the standard was shown in Fig 2. FIG 2: STANDARD CHROMATOGRAM International Journal of Pharmaceutical Sciences and Research 3697
Khairnar et al, IJPSR, 2014; Vol. 5(9): 3696-3703. E-ISSN: 0975-8232; P-ISSN: 2320-5148 Sample preparation The 220mg powder of Ketrol - DT contain 10mg Ketorolac tromethamine take in 10 ml volumetric flask and make up the volume with methanol. Sonicated for 15 min and filtered through 0.45 µm nylon membrane filter. A representative chromatogram of the sample was shown in the Fig 3 and Table 1. Evaluation of System Suitability Evaluation of System Suitability The 20 µl of standard solution was injected in six duplicate before and after the analysis and the chromatogram were recorded. System suitability parameter like column efficiency, plate count and tailing factor were also recorded. The column efficiency was determined was found to be more than 2000 USP plate count, USP Tailing for the same peak is not more than 2.0 and % RSD of six injection of the standard solution is not more than 2.0% the chromatogram was shown in Fig.4 and Table 2. FIG 3: SAMPLE CHROMATOGRAM TABLE 1: ANALYSIS OF MARKETED FORMULATION Commercial Ingredients Labeled Formulation amount Ketrol DT Ketrol DT Ketorolac Tromethamine Ketorolac Tromethamine Area Amount found % found (mg) (mg) 10 mg 365.8 10.14 100.70 10 mg 368.75 10.32 101.60 FIG.4: SYSTEM SUITABILITY CHROMATOGRAM TABLE 2: SYSTEM SUITABILITY STUDY Injections USP Plate count USP Tailing Factor RT Peak Area (min) 1 8219 1.22 7.700 450.25 2 8362 1.35 7.766 453.17 3 8398 1.22 7.783 462.70 4 8219 1.22 7.700 460.36 5 6943 1.22 7.666 457.58 6 8324 1.25 7.712 452.77 Mean 7.721 456.13 SD 0.0444 4.8535 %RSD 0.5750 1.064 International Journal of Pharmaceutical Sciences and Research 3698
ANALYTICAL METHOD VALIDATION: Linearity The linearity of Ketorolac Tromethamine was determined by preparing and injecting solution with concentration of about 5-25 µgm/ml. the calibration curve indicates the response is linear over the concentration range studied for Ketorolac Tromethamine with correlation coefficient (r) of 0.999. Calibration curve for linearity shown in Fig. 5 and their values in Table 3. TABLE 3: LINEARITY Sr. No. Conc. Area Area Mean SD % RSD (µgm/ml) I II 1 5 103.12 106.00 104.56 2.04 1.95 2 10 195.70 192.72 194.21 2.11 1.08 3 15 285.38 283.32 284.35 1.46 0.81 4 20 357.56 362.75 360.16 3.67 1.02 5 25 450.39 444.36 447.38 4.26 0.85 FIG. 5: CALIBRATION CURVE FOR KETOROLAC TROMETHAMINE Precision Precision was measured in terms of repeatability of application and measurement. Repeatability of standard application ( system precision) was carried out using six replicate of the sample injection (25µgm/ml).repeatability of sample measurement ( method precision ) was carried out in six replicate of sample preparation from the same homogenous blend of the marketed sample (25 µgm/ml). The percentage RSD for repeatability of standard preparation was 1.18% where as the % RSD for repeatability of the sample 0.79%. This shows that the precision of the method is satisfactory as percentage RSD is not more than 2% the chromatogram was shown in Table 4 and 5. TABLE 4: PRECISION STUDY OF THE SYSTEM Sr. Sample % Assay Amount No. Area Present(µgm) 1 450.25 100.40 25.10 2 453.17 101.08 25.27 3 462.70 103.32 25.83 4 460.36 102.80 25.70 5 457.58 102.12 25.53 6 450.25 102.70 25.60 Mean 101.94 25.49 SD 1.20 0.30 % RSD 1.18 1.18 TABLE 5: PRECISION STUDY OF THE METHOD Sr. Sample Amount % Assay No. Area Present(µgm) 1 451.37 100.48 25.12 2 456.21 101.36 25.34 3 463.67 101.80 25.45 4 459.36 102.60 25.65 5 456.48 101.72 25.43 6 454.23 100.56 25.14 Mean 101.42 25.35 SD 0.8066 0.2016 % RSD 0.795 0.795 The inter-day precision also carry out using 10, 15 and 25 µgm/ml standard solution % RSD found is not more than 2% shown in Figure 6 and Table 6. FIG 6: PRECISION CHROMATOGRAM International Journal of Pharmaceutical Sciences and Research 3699
TABLE 6: PRECISION (INTERDAY) Accuracy The percentage recovery experiments were performed by adding known quantity of pure standard drug into the pre-analysed sample. The solution equivalent to 100mg of Ketorolac tromethamine was accurately weight into a 100ml volumetric flask. The sample was then spiked with standard at level 80 %, 100 % and 120 % of test TABLE 7: ACCURACY STUDY Sr. No. Conc. Area I Area II Mean SD RSD 1 10 194.16 192.93 193.55 0.87 0.45 2 15 277.23 283.71 280.47 4.58 1.63 3 20 441.46 445.94 443.70 3.17 0.71 Spiked Amount added Peak Amount Percent level µgm/ml area found recovery 80% 341.98 18.75 104.1 concentration. The resulting spiked sample solutions were assayed in triplicate and the results were compared and express as percentage. The mean percentage recovery of Ketorolac Tromethamine was found to be in range between 101.7 and 103.6 which are within the acceptance limit was shown in the Table 7 and Fig 7. 80% 8 344.55 18.90 105.0 103.6 80% 343.25 18.35 101.9 100% 372.86 20.56 102.8 100% 10 365.37 20.12 100.6 101.7 100% 368.42 20.39 101.9 120% 409.56 22.71 103.2 120% 12 405.88 22.50 102.2 102.8 120% 407.29 22.67 103.0 Percent mean recovery FIG 7: ACCURACY CHROMATOGRAM TABLE 8: ROBUSTNESS STUDIES System suitability parameter ( variation) % RSD of peak area response (n=3) Robustness Robustness of the method was determine by analyzing standard solution at normal operating condition by changing some operating analytical conditions such as flow rate, mobile phase and detection wavelength. The condition with variation and there result were shown in Table 8. The tailing factor is around indicative of peak symmetry and theoretical plate count also above 2000. Hence robustness of the extend of variations applied to analytical condition was shown in Fig 8. Mean tailing factor (n=3) Mean retention time (n=3) Flow change 0.9 ml/min 1.61 1.27 8.23 1.1 ml/min 1.29 1.25 6.68 Mobile phase volume 64:36 1.81 1.18 7.73 66:34 1.56 1.15 7.15 Wavelength change 244 nm 1.18 1.20 7.67 246 nm 1.87 1.18 7.33 Limit of detection (LOD) and Limit of quantification (LOQ) The limit of detection (LOD) is a lowest amount of analyte in a sample that can be detected, but not International Journal of Pharmaceutical Sciences and Research 3700
necessarily quantified, under the stated experimental conditions. LOD and LOQ was calculated by using standard deviation and slope value obtained from calibration curve by using formula LOD=3.3(SD/S) and LOQ=10(SD/D). The LOD and LOQ value for Ketorolac Tromethamine was found to be 0.825 µgm/ml and 2.501 µgm/ml respectively. Force Degradation study Thermal Degradation Heat about 1000 mg of tablet powder at 105 0 c for 24 hr. weigh accurately this powder equivalent to 100 mg of Ketorolac Tromethamine into a 100ml volumetric flask added 60 ml of diluent and sonicate 15 min with intermittent shaking and make up to the mark with diluent. Thermal degradation study was carried out after 1day, 3day and 6day shown in Fig 9. Acid degradation Weight accurately tablets powder equivalent to 100 mg of Ketorolac tromethamine into 100 ml volumetric flask add 10 ml of 5 N Hydrochloric acid heat it on water bath at 80 0 c for 8 hr, cool it add 10 ml of 5N Sodium Hydroxide and add 60 ml of diluent and sonicate, dissolved the substances, make up to the mark with diluent and mix well. Filter the solution through 0.45 µ nylon filter. The Chromatogram is shown in Fig 11. FIG 9: THERMAL STRESS CONDITION Photo stability Expose about 1000mg of tablet powder in photo stability for 1.2 million Lux hr. weigh accurately this powder equivalent to 100 mg of Ketorolac tromethamine into a 100 ml volumetric flask add 60 ml of diluent and sonicate for 15 min with intermittent shaking and make up to the mark with diluent. Filter the solution through 0.45µ nylon filter. Photo stability was carried out after 1 day, 3day and 6 day shown in Fig 10. FIG 11: ACID STRESS CONDITION Base degradation Weight accurately tablets powder equivalent to 100 mg of Ketorolac tromethamine into 100 ml volumetric flask add 10 ml of 5 N Sodium Hydroxide heat it on water bath at 80 0 c for 8 hr, cool it add 10 ml of 5N Hydrochloric acid and add 60 ml of diluent and sonicate, dissolved the substances, make up to the mark with diluent and mix well. Filter the solution through 0.45 µ nylon filter. Chromatogram shown in Fig 12. FIG 10: PHOTO DEGRADATION STUDY CHROMATOGRAM FIG 12: BASE STRESS CONDITION CHROMATOGRAM Peroxide Degradation Weight accurately tablets powder equivalent to 100 mg of Ketorolac tromethamine into 100 ml volumetric flask add 10 ml 30% hydrogen peroxide heat it on water bath at 80 0 c for 8 hr, add 60 ml of diluent and sonicate, dissolved the substances, make up to the mark with diluent and mix well. International Journal of Pharmaceutical Sciences and Research 3701
Filter the solution through 0.45 µ nylon filter. Chromatogram and force degradation data as has shown in Fig 13 and Table 9 respectively. FIG 13: PEROXIDE STRESS CONDITION TABLE 9: FORCE DEGRADATION DATA Treated Parameter RT Theoretical plate As such Ketorolac 7.88 8615.4 1.27 Thermal treatment After 1day 7.41 6497.6 1.12 3 day 7.40 6468.4 1.16 6 day 7.33 6352.4 1.12 Photo Stability After 1day 3 day 6 day 7.40 7.43 7.36 6468.4 6526.8 6410.4 Tailing factor 1.12 1.12 1.05 Acid heat 7.50 6644.4 1.12 treatment Base after heating 7.45 7694.3 1.12 Oxidation treatment after heat 7.71 6064.9 1.11 RESULTS AND DISCUSSION: A different combination of mobile phases and chromatographic conditions were tried and a mobile phase containing methanol and water with 0.1% O- phosphoric acid (65:35 V/V), Grace C18 (250 cm x 4.6 mm x 5 μ) column, 1.0 mlmin-1 flow rate, 20 μl injection volume, 30 0 C column oven temperature, 245 nm wavelength and 16 min run time was found to be suitable for all combinations. These chromatographic conditions gave retention time of 7.70 min. The force degradation study of the sample solution was evaluated by preparing a sample solution as per the proposed method and analyzed after 1 day,3day and 6 day for thermal and photo stability study retention time found same as per standard chromatogram. System precision and method precision results showed the % RSD of 1.18 and 0.79, respectively. A good linearity relationship indicated by correlation coefficient (r) value 0.999 was observed between the concentrations of 5μgmL-1 to 25 μgml-1 of Ketorolac Tromethamine. Inter-day Precision was done by changing the analyst, column, with the same chromatographic conditions and the obtained results were within the limits. The Robustness method was evaluated by deliberately varying the chromatographic conditions of the method such as mobile phase methanol content, flow rate and wavelength. The parameter like tailing factor and retention time showed adherence to the limits. The accuracy of the method was determined and the percentage recovery was calculated. The data indicates an average of 103.6 % recovery of the standard sample. CONCLUSION: The method developed for Ketorolac Tromethamine was found to be simple process and the procedure does not involve any experimental conditions. The validation results indicated that the method was specific, accurate, linear, precise, rugged and robust. The runtime was relatively 20 min which enabled rapid quantification of many samples in routine and quality control analysis of tablet formulation. ACKNOWLEDGEMENT: We are thankful to Prof. D.V. Derle Principal of M.V.P. Samaj s College of Pharmacy, Nashik for providing the facility to carry out the research work. REFERENCES: 1. Sunil G, Jambulingam M, Thangadurai AS, Kamalakannan D, Sundaraganapathy R, Jothimanivannan C: Development and Validation of Ketorolac Tromethamine in Eye Drop Formulation by RP-HPLC Method. Arabian Journal of Chemistry 2012; 1-21. 2. Babu NB, Rao SP, Raju RR: RP HPLC Method Development and Validation of Acuvail Drug. International Journal of Research in Pharmaceutical and Biomedical Sciences 2011; 2(1): 128-129. 3. Vijaya V, Vrushali T, Joshi SV, Dhole SN: New Simultaneous UV-Visible Spectrophotometric Methods for Estimation of Ofloxacin and Ketorolac Tromethamine in Ophthalmic Dosage Form. Asian J. Pharm. Ana 2013; 3 (2): 53-57. 4. Raja B, Rao AL: Analytical method development and validation for the simultaneous estimation of Febuxostat and Ketorolac in tablet dosage forms by RP-HPLC. International Journal of Pharmaceutical, Chemical and Biological Sciences 2013; 3(3): 571-576. 5. Connor ON, Geary M, Wharton M, Curtin L: Development and validation of a rapid liquid chromatographic method for the analysis of Ketorolac Tromethamine and its related production impurities. Journal of Applied Pharmaceutical Science 2012; 2(5): 15-21. 6. Vaibhav S, Mohit M, Sadhana R: Validation of RP- HPLC method for simultaneous estimation of febuxostat and Diclofenac potassium in bulk drug and in bilayer tablet International Journal of Pharmaceutical Sciences and Research 3702
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