Get the most out of Pure PRP

Get the most out of Pure PRP

The Basic Science of PRP Platelets are small non-nucleated bodies in peripheral blood known for role in hemostasis Platelets contain proteins, cytokines & other bioactive factors that initiate & regulate basic aspects of wound healing Normal platelet counts in blood range from 150,000/µl to 350,000/µl Plasma is fluid portion of blood & contains clotting factors & other proteins & ions

Whole blood is collected PRP Preparation Anti-coagulating agent is added (sodium citrate) Centrifuge separates blood components Spinning blood causes three layers Red blood cells, Buffy coat, Plasma Desirable bits are used How much plasma determines concentration How much into the buffy coat determines white blood cell concentration Coagulating agent may be added (with ProTec) CaCl to counteract anti-coagulant added and form fibrin matrix

ProTec is: Consistent and Easy to Use Convenient Cost effective Very easy to invest in for the practicing veterinarian Good Science Pure, Protected Platelet Technology

ProTec Features and Benefits Separator Gel in Collection Tube Physically separates Platelets and Plasma from Red and White Blood Cells takes the guess work out of PRP preparation Consistent preparation every time Single Spin Process quick and easy (9 ml of blood, 6 minute spin, yields 4-5 ml of PRP) Chemically balanced Anti-coagulant (Sodium Citrate) and Coagulant (Calcium Chloride) Ensures no unnecessary activation of platelets during coagulation process

ProTec Features and Benefits Convenient and Inexpensive Centrifuge with travel case Rugged, dependable, inexpensive centrifuge for inclinic and remote treatments

ProTec Features and Benefits ProTec will form a protective fibrin matrix in vivo following injection: Fibrinogen in plasma forms fibrin fibrin is released following injection and adheres to collagen fibers in an overlapping matrix formation growth factors released from platelets are held in matrix and gradually released to stimulate healing

Kit Configuration

Kit Components Vacutainer 21G Blood Collection Needle and Holder Plasma Tube with Sodium Citrate and Separator Gel Transfer Device Vial with CaCl2 Solution Syringe 21G Injection Needle

Maintain Sterile Field Prepare horse for blood draw (neck)

Vacutainer and Needle

Prepare 21G 1 Butterfly Needle* with vacutainer tube Insert Needle into Vein

Plasma Tube

Connect vacutainer tube to plasma tube Withdraw (using vacuum) 9mL of venous blood (don t drop the plasma tube!) Disconnect Plasma Tube from Vacutainer Tube

Remove needle and slide cover over needle Wipe top of plasma tube with disinfectant Gently invert plasma tube 7X to mix blood thoroughly with anti-coagulant Sodium Citrate

Place plasma tube in centrifuge with counter balance tube, and close centrifuge lid Press Yellow start button on the centrifuge (6 minute spin) Prepare horse for injection

Remove plasma tube with separated blood components from centrifuge Gently invert plasma tube 7X to ensure mixing of plasma and platelets

Transfer Device and Coagulant Vial

Remove the cap from the vial containing Calcium Chloride (CaCl 2 ) solution Attach the transfer device to the vial Invert the plasma tube and attach it to the transfer device Transfer approximately 5mL (all) of plasma and platelet mixture to vial (using vacuum)

Remove the plasma tube from the transfer device and vial Gently invert the vial 7X to ensure mixing of ProTec with CaCl 2 coagulant Note that once Plasma mixes with CaCl 2, it will coagulate in 10 15 minutes

Syringe

Detach the transfer device from the vial Attach the syringe to the vial Withdraw the ProTec into the syringe Detach the syringe from the vial

Injection Needle

Attach the 21G 1 needle to the syringe* Expose needle and inject After use, cover the needle with safety sheath Tidy up!

Primary Indications in Veterinary Medicine Tendon and Ligament injuries (acute and chronic) Primarily injected into core lesions Also applied surgically as a gel or membrane Bone healing Joints Wound healing Topical gel application

Precautions Ensure aseptic/sterile technique is maintained throughout the procedure. ProTec is a closed-system designed to allow single clinician operation while maintaining aseptic technique, but thought must be given to this at all times Once the PRP solution is drawn into the Calcium Chloride vial, the coagulation process will begin. Injection site preparation should be conducted during the 6 minute centrifuge spin cycle The PRP solution will become viscous in about ten minutes, but there should be plenty of time to inject the PRP prior to this happening.