Complete Solutions for Small Animal Infusion

Complete Solutions for Small Animal Infusion NEW! Full Line of Catheters Swivels and Infusion Cages Vascular Access Products and Tethers Tubing and Connectors Jackets Syringe Pumps Surgical Instruments

Catheters Catheters for Mice and Rats New Product! Jugular Vein Femoral Vein Femoral Artery Carotid Artery Tail Vein Intrathecal Bile Duct Gastric Portal Vein Key Features: Designed with rounded tips, suture collars and other features specifically for the indicated species and vessel Utilizes tubing that has been custom-extruded for an ideal fit with the Vascular Access Harnesses, Buttons, and PinPorts Made using high quality medical-grade materials and advanced catheter manufacturing equipment Easily customizable Size Length Collars Mouse Jugular Vein, polyurethane, fits 25 ga 75-0831* 1-3Fr 10.5 cm 1.2 cm 75-0401 2Fr 10 cm 1.3 cm 75-0832 2Fr 6 cm 1.3 cm 75-0833 2Fr 8 cm 1.0 cm 75-0834 2Fr 4 cm 1.3 cm 75-0835 2Fr 6 cm 1.0 cm Mouse Femoral Artery/Vein, polyurethane, fits 25 ga 75-0842 1Fr to VAH tubing 54.5 cm 2.0 cm 75-0843 1Fr to VAH tubing 54 cm 2.0 cm Mouse Carotid Artery, polyurethane, fits 25 ga 75-0402* 1-3Fr 8 cm 1.1 cm 75-0847 2Fr 6 cm 0.9 cm Mouse Tail Vein, Pebax tubing, includes stylet and PinPort with injector 75-0858 29 ga 25 cm n/a 75-0859 29 ga 5 cm n/a * fits 22 ga Size Length Collars Rat Jugular Vein, polyurethane, fits 22 ga 75-0403 3Fr 30 cm 2.5 and 3.0 cm 75-0820 3Fr 15 cm 3.0 cm 75-0821 3Fr 25 cm 3.0 cm 75-0822 3Fr 20 cm 2.5 and 2.8 cm 75-0823 3Fr 10 cm 2.5 cm 75-0824 3Fr 60 cm 2.5 cm 75-0825 3Fr 21 cm 4.0 and 5.2 cm 75-0826 3Fr 10 cm 3.0 cm 75-0827 3Fr 12 cm 3.8 cm 75-0828 3Fr 60 cm 4.5 cm 75-0829 3Fr 20 cm 2.0 cm 75-0830 3Fr 25 cm 3.0 and 15.0 cm Rat Femoral Artery, polyurethane, fits 22 ga 75-0836 1-3Fr 30 cm 1.2 cm Rat Femoral Vein, polyurethane, fits 22 ga 75-0837 1.9-3Fr 29.5 cm 4.5 and 7.5 cm 75-0838 1.9-3Fr 29.5 cm 4.0, 4.5, and 7.5 cm 75-0839 3Fr 25 cm 3.0 and 3.5 cm 75-0840 3Fr 20 cm 3.5 and 6.0 cm 75-0841 3Fr 30 cm 5.0 cm Rat Carotid Artery, polyurethane, fits 22 ga 75-0404 1.9-3Fr 16.5 cm 3.5 and 8.0 cm 75-0844 1.9-3Fr 13.5 cm 3.5 and 8.0 cm 75-0845 2Fr to VAH tubing 29 cm 1.5 cm 75-0846 3Fr 12 cm 2.0 cm Rat Intrathecal, polyurethane, includes stylet and PinPort with injector 75-0848 32 ga 14 cm n/a 75-0849 32 ga 14.5 cm n/a 75-0850 32 ga 18 cm n/a Rat Bile Duct, polyurethane, fits 22 ga 75-0851 2Fr 69.5 cm 1.0 cm 75-0852 3Fr 30 cm 0.7 and 5.0 cm 75-0853 3Fr 40 cm n/a Rat gastric, polyurethane, fits 22 ga 75-0854 3Fr 25 cm 2.5 cm Rat Portal Vein, polyurethane, fits 22 ga 75-0855 3Fr 20 cm 0.7 and 5.0 cm Rat Tail Vein, PE/PVC tubing, includes stylet and PinPort with injector 75-0856 25 ga 25 cm n/a 75-0857 25 ga 6 cm n/a 2

Small Animal Infusion Swivels ST1 61-0003 Dual Channel Swivel ST1 56-1324 Single Channel Swivel Stainless Steel Swivels Key Features: Lifetime warranty on single channel swivels Autoclavable single channel swivels Single and dual channel models available A swivel is a device that is inserted into a fluid path so that the infusion line can rotate as the animal moves. It is a critical part of any tethered animal infusion system. The Instech fluid swivels have been in laboratory use for forty years and have earned a reputation as the finest in the industry. Single Channel Swivels Single channel stainless steel swivels are autoclavable and may be reused hundreds of times. Self-adjusting seals, at the heart of every swivel, actually improve with use. All stainless steel models include a universal swivel-to-tether clamp which mates with any of our spring tethers. Dual Channel Swivels A two channel swivel gives you the ability to infuse and withdraw simultaneously to infuse on one channel while monitoring on the other, or to infuse on two lines independently. Dual channel swivels are a bit more delicate than single channel models, particularly the side channel. With proper care and use, dual channel swivels can last for many years. Microdialysis swivels For years, the 61-0004 Dual Channel Swivel has been the industry standard for awake animal microdialysis. It features quartz lining on the center channel to minimize dead volumes and reactivity with neurotransmitters. The newer 72-0000 uses a completely different seal design with two significant improvements: quartz lining on both the center and side channels, and extremely low frictional torque, making it the first dual channel swivel that can be used with mice. Disposable polysulfone swivels also available in 20, 22, and 25 gauge. Check out our CMA line of microdialysis probes and fraction collectors! www.microdialysis.se Application Single Channel Swivels Dual Channel Swivels Microdialysis for Mice & Rats X Microdialysis for Rats X Standard Infusion of Mice X X Standard Infusion of Rats X X Specifications Inlet & Outlet Tube Gauge 22 ga 25 ga 22 ga 22 ga 22 ga 22 ga Center Channel ID 0.41 mm 0.25 mm 0.41 mm 0.15 mm 0.15 mm 0.25 mm Center Channel Dead Volume 8 µl 3 µl 8 µl 1.8 µl 1.4 µl 2 µl Side Channel ID n/a n/a 0.41 mm 0.15 mm 0.41 mm 0.41 mm Side Channel Dead Volume n/a n/a 18 µl 3 µl 18 µl 4 µl Size (OD x L) 9.5 x 57.1 mm 9.5 x 57.1 mm 9.5 x 60.4 mm 9.5 x 60.4 mm 9.5 x 60.4 mm 9.5 x 60.4 mm Autoclavable Yes Yes Yes No Yes No Materials in Fluid Path* SS, T SS, T SS, T (center) SS, T (side) Q, T (center) Q, T, P, PM (side) *NOTE: SS is corrosion-resistant stainless steel (304 and 316); P is polyssulfone plastic; T is PTFE; Q is fused silica quartz; PM is polyimide Q, T (center) Q, T (side) SS, T (center) SS, T (side) Order # for Swivel 61-0001 56-1324 61-0003 72-0000 61-0004 75-0277 3

Infusion Cages & Counter-Balanced Level Arms Rodent Infusion Cages No sharp corners Tethers less likely to tangle Probes less likely to dislodge Durable polycarbonate for long life Durabable polycarbonate cages designed to house tethered rodents during short-term infusion and microdialysis experiments. Since the cage is round, tethers will not tangle as they sometimes can in shoebox-type cages. Also, animals are less likely to dislodge sensitive probes since there are no sharp corners. Handles at the top make it easy to move the cage, and it interfaces flawlessly with the counter-balanced lever arms (see below). Product Rat 61-0042 Rat Infusion cage, polycarbonate, 38.1 cm high 61-0044 Rat Infusion Cage Cover 72-0017 Rat Infusion Cage with Pellet Feeder 72-0018 Rat Infusion Cage with Water Bottle 72-0019 Rat Infusion Cage with Pellet Feeder and Water Bottle Mouse 61-0046 Mouse Infusion Cage, polycarbonate, 21.6 cm high 61-0048 Mouse Infusion Cage Cover 72-0014 Mouse Infusion Cage with Pellet Feeder 72-0015 Mouse Infusion Cage with Water Bottle 72-0016 Mouse Infusion Cage with Pellet Feeder and Water Bottle 61-0047 Bottom Screen for Mouse Infusion Cage Counter-Balanced Lever Arm Minimizes Stress on tether and animal Unimpeded animal movement Designed to minimize stress on subject, the Instech Counter- Balanced Lever Arm moves with the animal to prevent slack in the tether. Most other lever arms use a mass as the counterbalance; ours uses an adjustable spring, making it lighter and more responsive. The mounting plate can be adjusted for mounting to the side or top of many types of cages, including the 61-0042 and 61-0046 Animal Infusion Cages (see above). If you are using a shoebox-type cage, a counter-balanced lever arm can prevent the tether from tangling. The 61-0023 is typically used for studies with rats; the 61-0024 is ideal for any continuous infusion study involving mice. Additional swivel mount options available. Contact Technical Support for more information. Product 61-0023 6 in Adjustable Spring Counter-Balanced Lever Arm 61-0024 3.5 in Adjustable Spring Counter-Balanced Lever Arm 4

Small Animal Infusion Vascular Access Harness Vascular Access Harness TM Septum permits aseptic technique Quick connection Minimal backflow Low profile Single and dual channel options available The Instech Vascular Access Harness (VAH), an advancement on the original Covance Infusion Harness, permits quick and aseptic connection and disconnection of a catheterized rat or mouse and an infusion tether. The VAH consists of a small external port (or two ports, in the case of the dual channel model) housed in a harness which is installed at the same time that the catheter is implanted. The catheter is attached to a connector built into the port under the harness dome. The port and catheter are filled with lock solution to maintain patency during transport. To begin an infusion or blood sampling study, simply remove the cap, wipe the septum with disinfectant and plug the mating VAH tether into the harness. A recessed septum-piercing needle built into the tether makes the fluid connection through the port. Like a subcutaneous access port, the VAH is a closed system; tether connection does not introduce contamination or air. Furthermore, retrograde flow, which can lead to occluded catheters, is virtually eliminated. Single Channel Vascular Access Harness Rat 72-9163 VAH with septum cover and septum needle 72-9164 VAH Tether Assembly, 30.5 cm 72-9165 Tether Assembly Kit: 72-9164 VAH Tether Assembly, 72-4075 22G Plastic Swivel, 60.9 cm CO-EX tubing and luer stubs 72-9166 VAH Septum Needle, female luer, 22 ga x 1.3 cm, pkg. of 12 72-9167 Rat VAH Connector with luer inlet, 3 cm tubing, pkg. of 12 75-0291 Plug for VAH Tether, pkg. of 10 Mouse 75-0065 VAH for Mice (injection set not included) 75-0062 VAH Assembly for Mice, 17.8 cm 75-0063 Tether Assembly Kit: 75-0062 VAH Tether Assembly, 72-4076 25 ga plasic swivel, 60 cm Co-EX tubing and 25 ga Luer stub 75-0061 Mouse VAH Connector with luer for direct access, pkg. of 12 Mouse VAH Connected Dual Channel Vascular Access Harness Needle Septum Catheter Attachment Rat 72-4454 Dual Channel VAH with septem needle 72-4455 Dual Channel VAH Tether Assembly, 30.5 cm 72-4456 VAHD Tether Assembly Kit: 72-4455 Dual Channel VAH Tether Assembly, 72-4070 Mini Tube Injection Port 22 ga x 4 mm, 61-0014 22 ga single-channel plastic swivel, 60 cm CO-EX tubing, 72-4439 Luer Stub 22 ga 75-0293 Protective Cap for VAHD Harness 75-0294 Dual VAH with 1 extra injection port and septum needle 75-0295 Dual VAH with 2 extra injection ports and septum needles 72-9166 VAH septum needle, female luer, 22 ga x 1.3 cm, pkg. of 12 Mouse 75-0811 Dual Channel Mouse VAH, 22 ga red, 22 ga white ports, injector, sterile 75-0812 Dual Channel Mouse VAH, 25 ga red, 22 ga white ports, injector, sterile 75-0813 Dual Channel Mouse VAH, 25 ga red, 25 ga white ports, injector, sterile 75-0814 Tether Assembly for Dual Channel Mouse VAH (75-0811, 75-0812, and 75-0813) 75-0815 Spring for Dual Channel Mouse Tethers, non-sterile, pkg. of 5 75-0816 Tether for Mouse VAH or VAB, 7 in, silicone sleeve for stainless steel swivel, sterile All supplied sterile and individually, unless otherwise noted. 5

Vascular Access Button and PinPorts BOTTOM VIEW Silicone Septum Polyester Surgical Felt Rigid Polyurethane Body Stainless Steel Couple Vascular Access Button TM Features an external port with a septum for closed systems Ideal for longer-term studies Available for rats and mice Button tethers are surgically implanted under the skin. In older models the catheter passes through an opening in the stalk of the button through a spring tether and up to a swivel. The Instech Vascular Access Button (VAB) features an external port with a septum to create a closed system. Compared to harnesses, buttons require a bit more work in surgery, however, there are no belly bands that require adjustment and with proper tissue ingrowth, buttons can be a good option for longer-term studies. All supplied sterile and individually, unless otherwise noted. Rat 75-0056 VAB for Rat with septum needle 75-0057 VAB Tether Assembly, 30.5 cm 75-0058 Tether Assembly Kit: 75-0057 VAB Thether Assembly, 72-4075 22 ga plastic swivel, 60 cm CO-EX tubing, 72-4439 luer stub 75-0059 Protective Metal Cap for Rat VAB 72-9166 VAH Septum Needle, 22 ga x 0.5in, pkg. of 12 Mouse 75-0060 VAB for Mice, 22 ga 75-0061 Mouse VAB Connector with luer for direct access, pkg. of 12 75-0062 VAB Tether Assembly for Mice, 17.8 cm 75-0063 Tether Assembly Kit: 75-0062 VAB Tether Assembly, 72-6121 25 ga plastic swivel, 60.9 cm PU external infusion tubing, 25 ga luer stub PinPorts TM for Catheters and Tubing Infuse or withdraw from the catheter in a fraction of the time it takes with plugs and blunt needles The PinPort is a closed system that permits aseptic technique leading to reduced infection and improved patency The injector is recessed and provides complete needle stick protection for the user New Product! The Instech PinPort provides fast, aseptic access to externalized rodent catheters or tubing for dosing or blood sampling. It is designed for intermittent, manual access; it is not designed to be connected to a tether and swivel for removal or continuous access (see Vascular Access Harness or Vascular Access Button). The PinPort is available in white or red with 22 ga or 25 ga couples. Colors can be used to distinguish vessels. One injector works with all PinPort varieties. Quantity 75-0220 PinPorts 22 ga, white, with injector pkg. of 20 75-0221 PinPorts 22 ga, red, with injector pkg. of 20 75-0222 PinPorts 25 ga, white, with injector pkg. of 20 75-0223 PinPorts 25 ga, red, with injector pkg. of 20 75-0224 PinPort injectors case of 50 75-0225 PinPorts connector, male inlet, 22 ga each 75-0226 PinPorts connector, male inlet, 25 ga each 75-0227 PinPort 22 ga, white, 16 mm long knurled with groove for head attachment, 22 ga x 5 mm tubing, which injector each 75-0228 PinPort 22 ga, white, non-magnetic Nitinol tubes, with injector box of 20 6

Small Animal Infusion Tubing & Connectors Polyethylene Tubing Supplied Non-sterile. Larger sizes available. Not Autoclavable; can be ETO sterilized. Tubing Length Tubing Length PE Diameter 3 m 3.5 m # ID x OD mm 598321 598322 10 0.28 x 0.61 598323 598324 20 0.38 x 1.09 720191 720192-0.40 x 0.80 598325 598326 50 0.58 x 0.96 598329 598330 90 0.86 x 1.27 CO-EX External Infusion Tubing Luer Stubs Female Luer stubs (blunt needles) can be used to connect infusion tubing or catheters to syringes or other male Luer connectors. They are supplied in a non-sterile package of 100 (larger quantities available upon request). Additional sizes available. 72-4470 Luer Stub, 21 ga (purple) x 12 mm 72-4439 Luer Stub, 22 ga (blue) x 12 mm 72-4471 Luer Stub, 22 ga (blue) x 6 mm 72-4472 Luer Stub, 23 ga (orange) x 12 mm 72-6125 Luer Stub, 25 (red) ga (purple) x 12 mm 72-2847 Luer Stub, 27 ga (gray) x 12 mm CO-EX was developed to address the problem in drug infusion studies whereby compounds can be lost due to absorption into the tubing, evaporation through the tubing, or the tubing is altered due to the leaching of plasticizers from the tubing. CO-EX has PE on the ID fluid path for compatibility and PVC on the OD which adds thickness to the tubing to minimize kinking. Not designed for intravascular use. Supplied in a non-sterile 30.5 meter roll. Not autoclavable; can be ETO sterilized. 72-4426 CO-EX External Infusion Tubing, 22 ga 72-6122 CO-EX External Infusion Tubing, 25 ga Polyethylene Intravascular Tubing Supplied non-sterile. Not autoclavable; can be ETO sterilized. Size (ID x OD mm) 75-0216 PU tubing, 32 ga (0.127 x 0.254), 10 m spool 75-0217 PU tubing, 1Fr (0.178 x 0.356), 10 m spool 72-6130 PU tubing, 2Fr (0.33 x 0.66), 9 pieces of 3.65 m each, 33 m total 72-4429 PU tubing, 3Fr (0.61 x 0.97), 9 pieces of 3.65 m each, 33 m total 72-4431 PU tubing, 3.5Fr (0.69 x 1.20), 9 pieces of 3.65 m each, 33 m total Instech Y and X Connectors These connectors feature three or four stainless steel tubes and a hub made of Kel-F (PCTFE, chemically resistant). The tubes extend approximately 6 mm from the hub. The dead space in the 22 ga version from one channel to the next is less than 3 µl. Supplied individually and sterile. 75-0066 Y connector, 22 ga 75-0067 Y connector, 25 ga 75-0212 X connector, 22 ga 75-0213 X connector, 25 ga Additional tubing and connectors available. Please contact Technical Support for full line of tubing and connectors 7

Rat, Hamster & Mouse Jackets ST1 62-0065 Mouse Jacket ST1 62-0058 Rat Jacket Hook and loop fasteners for simple easy closure Sizes available for rats, hamsters, and mice Manufactured with Lycra /spandex, these jackets are suitable for a wide range of continuous intravenous infusion applications. The Mouse Jackets have a double layer of Lycra /spandex for additional durability. The jackets close by means of a hook and loop fastener and supplemental hooks provide additional security and better fit. Standard inserts are included for the rat, the mouse and the hamster jackets. Dermal inserts can be purchased separately for both the rat and mouse jackets. These inserts are for studies where test articles are dermally applied. They are a non-occlusive covering for the application site that helps prevent oral ingestion while allowing in-line investigations to be carried out with ease. Jackets also available for larger species. All Jackets are customizable. Please contact Technical Support for more information 62-0064 Mouse Jacket, Small, Up to 25 g 62-0065 Mouse Jacket, Medium, 25 to 30 g 62-0066 Mouse Jacket, Large, 30+ g 62-0056 Rat Jacket, X-Small, 100 to 150 g 62-0057 Rat Jacket, Small, 150 to 250 g 62-0058 Rat Jacket, Medium, 250 to 350 g 62-0059 Rat Jacket, Large, 350 to 500 g 62-0060 Rat Jacket, X-Large, 500+ g 62-0061 Hamster Jacket, Small, Up to 100 g 62-0062 Hamster Jacket, Medium, 100 to 175 g 62-0063 Hamster Jacket, Large, 175+ g 62-0067 Rat Jacket Insert for Dermal Studies 62-0068 Mouse Jacket Insert for Dermal Studies 8

Small Animal Infusion Syringe Pumps Harvard Apparatus syringe pumps are the only choice when performing animal infusion experiments. Our PHD Ultra TM and Pump 11 Elite families provide low flow rates and precise volume infusion for animal drug and nutritional studies. The innovative touch screen user interface allows easy programming of simple to complex methods, all without the use of a PC. The pumps are also available with preprogrammed methods* that allow you to be up and running with the push of a button. Key Features: Smooth, accurate flow down to the pl/min Easy-to-use LCD color touch screen interface Available in infuse only and infuse/withdraw/ programmable configurations Easily program simple to complex methods without a PC* Accommodates syringe sizes 0.5 μl 140 ml (model dependent) Pump 11 Elite Nanomite Accommodates syringe size 0.5 ul 1 ml (model dependent) Ideal for remote or stereotaxic injections Flow rate range - 3.66 pl/min to 3.82 ml/min with 11 lbs. of adjustable force Pump consists of a control unit, injection unit and footswitch Pump 11 Elite/Pico Plus Elite Series Accommodates syringe size 0.5 ul 60 ml (model dependent) Pump 11 Elite provides flow rates down to 1.26 pl/min For applications requiring lower flow, the Pico Plus Elite can provide flow rates down to 0.54 pl/min 35 lbs. of adjustable linear force 70-4500 Pump 11 Elite Infusion Only Single Syringe 70-4501 Pump 11 Elite Infusion Only Dual Syringe 70-4504 Pump 11 Elite Infusion/Withdrawal Programmable Single Syringe 70-4505 Pump 11 Elite Infusion/Withdrawal Programmable Dual Syringe 70-4511 Pump 11 Pico Plus Elite Infusion/Withdrawal Programmable Single Syringe 70-4506 Pump 11 Pico Plus Elite Infusion/Withdrawal Programmable Dual Syringe 70-4507 Pump 11 Elite Nanomite Infusion/Withdrawal Programmable Single Syringe PHD Ultra TM Series Accommodates syringe size 0.5 ul 140 ml Optional multi-syringe rack PHD Ultra TM provides flow rates down to 1.5 pl/min 75 lbs. of adjustable linear force 70-3005 PHD ULTRA Infusion 70-3006 PHD ULTRA Infusion/Withdrawal 70-3007 PHD ULTRA Infusion/Withdrawal Programmable Syringe Rack Kits for PHD ULTRA 70-3021A 4 x 140 Multi-Syringe Rack for PHD ULTRA 70-3022A Microliter Rack for PHD ULTRA, independently holds 4 syringes 70-3024A 6/10 Multi-Syringe Rack for PHD ULTRA, independently holds 10 syringes Pump Accessories ( also compatable with Pump 11 Elite & Nanomite) 70-4000 RS-485 Cable for Pump-to-Pump Communication, 0.5 m (1.6 ft) 70-4002 USB Cable for PC-to-Pump Communication, 2 m (6.6 ft) 70-4004 RS-232 Cable for PC-to-Pump Communication, 9-pin D-sub, 2 m (6.6 ft) 70-2215 Footswitch (with Phono Plug) Harvard Apparatus syringe pumps are for research only. Not for use on clinical patient. *Infusion/Withdrawal Programmable units only 9

Surgical Products Harvard Apparatus offers a variety of surgical instrument kits designed for specific applications. These kits were designed to give you a maximum performance at an affordable price. The Harvard Apparatus brand surgical tools are forged and finished in German foundries from German surgical-grade steel in ISO 9001 facilities. These kits are comprised of a variety of instruments specifically suited for the target application. Harvard Apparatus also offers a full line of products to fit all your small animal surgical needs from anesthesia and ventilators to surgical instruments, operating tables and other surgical equipment. Please see our website or contact Technical Support for our complete offering of surgical products. 72-8994 Rodent Microdialysis Surgical Kit 72-8942 Rat Surgical Kit 72-8943 Mouse Surgical Kit 72-8940 Minor Surgical Kit 72-8941 Deluxe Major Surgical Kit 10