UMN Onsite Program WASTEWATER 101 Dr. Sara Heger sheger@umn.edu March 13, 2017 Water Resource Center, Onsite Sewage Treatment Program (OSTP) 1. Education for Professionals started in 1974 2. Education for Homeowners & Small Communities started in early 1990s 3. Ongoing research and demonstration supporting educational efforts Minnesota Land of how many lakes? 6,564 rivers and streams Boasts the coldest temp in lower 48 Embarrass MN -60 F Feb1996 Produces General Mills Minnesota Mining & Manufacturing Minnesota Celebrities Prince, Bob Dylan, Jessica Lange, Judy Garland, Al Franken, Charles Schulz Movies Fargo, Grumpy Old Men Sources of Wastewater Presentation Overview Defined: Used water and water-carried solids WASTEWATER WHAT IS IT AND HOW DO WE TREAT IT? VARYING CONTAMINANTS POTENTIAL NEGATIVE AFFECTS LABORATORY MEASUREMENTS Domestic/Residential Commercial Industrial Heger Wastewater 101 1
Typical Water Use Where Does it Come From? 125-150 gallons per day per bedroom Assumes 2 people per bedroom 50-80 gallons/person/day Annual estimates of use Per person per year = 28,000 gal Typical home ~ 3 persons = 82,000 gal/yr 250 homes in a township = 20 million gallons/year Water use: Bathroom ~ 64% Toilet = 27% Bathing = 19% Faucets = 8% Laundry = 22% Kitchen = 10% Leaks = 14% Mayer, et al. Residential End Uses of Water. 1999. 1,188 homes, Total gpcd = 69.3 Wastewater Treatment So What s in Graywater? ALL WASTEWATER MUST BE TREATED: GREYWATER BLACKWATER What is NOT Sewage Clear water Sump pumps Water softener recharge water and other water treatment device clean water Treated water (hot tubs and pools) What to do with it? On surface or below surface in separate trench Do not but on neighbors property or into lake, river or stream Recommended setback from wells is 20 ft Sewage Constituents Organic Loading Biochemical Oxygen Demand (BOD) Total suspended Solids (TSS) Fat, Oils and Grease (FOG) Pathogens Fecal Coliform Viruses Nutrients Phosphorus Nitrogen Chemicals Heger Wastewater 101 2
Biological Treatment in Septic Systems Biological Treatment Processes (cont.) Uses the microorganisms present Different applications Biological population and processes dependent on system conditions Aerobic Facultative Anaerobic Biological oxidation Bacteria break down organic matter into water and CO 2 Reduces BOD, pathogens Works most efficiently in aerobic conditions All Systems Need Healthy Bugs Anaerobic Microbes Range DO ph Temp Anaerobic bacteria grow in absence of free oxygen,o2 Most anaerobic organisms are bacteria Anaerobic treatment processes split oxygen bound, Ex. SO 4: H 2 S, NO 3 N 2 Common condition in: Septic tanks Processing tanks Constructed wetlands Other saturated environments Anaerobes Cont d Anaerobic Digestion They are not able to get as much energy from their food Advantages: Microbes that do not require oxygen are able to live in places where aerobes cannot survive, such as the human gut, and many other places where oxygen is in low supply For pathogenic microbes (those that cause disease), this ability is a huge advantage, allowing anaerobic pathogens to cause disease in areas of the body that are not exposed to oxygen ORGANIC MATTER GASES + HUMUS CO2 CH4 H2S NH3 Heger Wastewater 101 3
Denitrification Anaerobic Microbes Aerobic Microorganisms N 2 gas NO 3 Aerobic bacteria require O 2 to live and grow Aerobic bacteria manage the chemical process by converting the inputs into heat, carbon dioxide and ammonium The ammonium is further converted by bacteria into nitrate Common condition in Soil treatment systems Media filters ATUs Microbes as Workhorses Organics, Microbes & Oxygen Microorganisms are used aerobic process is 10-20 times faster than anaerobic to convert colloidal and dissolved carbonaceous organic matter into various gases and into cell tissue gases evolve (CO2, N2, and others) new cells can be settled thus carbon is removed break other nutrients out of organic compounds nitrogenous compounds phosphorous species Bioavailable organic compounds provide food and energy to microbes Naturally-occurring microorganisms consume food, and create more microorganisms The more microorganisms, the more food consumed The more food consumed, more dissolved oxygen is required Basic Equation Carbon Removal Respiration CO 2 O2 H 2 O Heger Wastewater 101 4
Processes Occurring Under Aerobic Conditions Facultative Microbes Nitrification: Transformation of NH 4 to NO 3 Nutrient reduction Pathogen removal TSS and BOD reduction Can develop in the presence of oxygen but does not require it The concentrations of oxygen and fermentable material in the environment influence the organism's use of aerobic respiration vs. fermentation to derive energy If given the choice they prefer to have access to oxygen, in order to get the maximal amount of energy from metabolizing their food Microbes Minimal Requirements Appropriate oxygen supply Temperature must be life-sustaining ph needs to be controlled Steady supply of food to maintain stable microbial population Limited biocides Oxygen States - Dissolved Dissolved Free O 2 Oxygen that has been incorporated into water Oxygen source for aerobic and facultative microbes Under aerobic conditions, bacteria rapidly consume organic matter and convert it into carbon dioxide Dissolved Oxygen (DO) Amount of Dissolved Oxygen Concentration of O2 dissolved in water Measure with meter and probe or colorimetric kit in field DO expressed as mg/l DO low in septic tank <1 mg/l DO >2 mg/l from advanced treatment systems Organic material If oxygen is available, organic material requires oxygen to decompose Organic material may also decompose in the absence of oxygen More organic material requires more DO, and will tend to deplete water of DO Oxygen demand The oxygen demand is the amount of oxygen required to aerobically oxidize a material Heger Wastewater 101 5
Organic Solids Biochemical Oxygen Demand What? Digested and undigested animal and vegetable material Synthetic (artificial) organic compounds Laundering and bathing Impacts? Requires oxygen to be broken down Biochemical Oxygen Demand (BOD) Lowers water quality in lakes, rivers, and streams How treated? Removed and stored in septic tank Effluent screen helps reduce further Serves as food source for good soil bacteria in soil treatment system Biochemical oxygen demand (BOD) is the amount of oxygen used by organisms during the breakdown of organic material BOD is considered an indirect measure of the organic content of a sample Chemical Oxygen Demand DO ranges for Microbes Another means of measuring oxygen demand needed to oxidize organics and reduced nitrogenous compounds Faster than BOD Always higher than BOD COD is much higher than BOD in raw wastewaters COD:BOD ratio is usually less than 2:1 in treated effluents COD:BOD ratio is dependent on circumstances and reduces with treatment Ideal Dissolved Oxygen Range in Wastewater Microbes Anaerobe Facultative Aerobe Low DO (mg/l) High DO (mg/l) Typical (mg/l) 0 0 0.5 0.5 5 5 0-0.3 0-1 1-3 Where Does DO Come From? Total Suspended Solids (TSS) Source water Groundwater Passive from air Active forced into system Measures both organic and inorganic solids which have not settled out (lint, dirt, etc) Typical home is <60 mg/l Settled in the tank & filtered in soil Heger Wastewater 101 6
Where Does TSS Come From? Organic matter (garbage disposal) Toilet paper Lint Dirt Other solids Garbage Disposal Problems: Adds more solids Undigested food Chopped into small pieces More water Recommendation: Don t install one Don t use it if you have one Recommended to add 50% more septic tank capacity with multiple compartments Effluent screen and alarm on outlet of septic tank INCREASES THE NEED FOR CARE and MAINTENANCE Measuring TSS Relationships in Biomat Run sample thru 0.45 micron filter Solids remaining on filter are SS Solids passing through are dissolved Fats, oils, & grease Fats Fats, oils, & grease Fats are animal based and solid at room temperature Oil are vegetable based and liquid at room temperature Grease is petroleum & burnt fats and oils Typical home is <20 mg/l Must be collected in glass jar Origin: Animal fats State: solid at room temperature Treatment: separate into scum, microbial degrade (4 times more energy than BOD) Heger Wastewater 101 7
Oils Grease Origin: Vegetable or plant State: liquid or solid at room temperature Treatment: separate into scum, microbial degrade (12 times more energy than BOD) Origin: Petroleum product State: solid or liquid at room temperature Treatment: separate into scum, toxic to microbial activity Can be toxic Temperature Temperature Influences Typical range: 48 70 F Typical value: 59 F Low end concerns Microbes become dormant below 41 F Nitrification impacted by temps below 50 F Harmful bacteria and viruses live longer in cold soils High end concerns < 74o Fats solidify Sample collected on a Saturday afternoon, temperature 102 Temperature has dropped to 95 Temperature and Growth Growth rates increase with increasing temperature Growth rates approximately double for a 50 ºF rise in temperature Temperature extremes may interfere with metabolic processes or harm the organisms What Influences Temperature Water temperature Holding time Air temperature Venting Heger Wastewater 101 8
Pathogens Fecal Coliform indicator of potential viruses/bacteria that is easy to measure and is found in the feces of all warm blooded animals Treatment Absorption Bacteria have positive change and soil has negative so attracted to each other Filtered Soil is sticky Killed Off Wastewater - Organisms Source Human waste Laundering/bathing Food waste Concern - Human health Amounts Range Typical Total Coliform Bacteria 10 8 10 10 10 9 CFU/100mL Fecal Coliform Bacteria 10 6 10 8 10 7 CFU/100mL CFU = colony-forming unit Pathogens How are Pathogens Treated? What? Virus, Bacteria, Helmiths (worms), Protozoa Impacts? Human health How treated? Difficulty living in oxygen rich environments Removal and die off in soil treatment system Unsaturated soil Oxygen present = Aerobic environment Bacteria come to eat food, develop biomat Biomat provides unsaturated flow From: Urine Food Household detergents Laboratory analysis for total phosphorous Impacts? Weed & algal growth in lakes, ponds, and streams P is limiting factor for most aquatic ecosystems Small amount of a P input results in large growth of algae Phosphorus (P) Phosphorus (P) Treatment P attaches to other minerals (iron and aluminum) in the soil It then can t move P will move if the soil moves (erosion) Some of the P is used by the vegetation Soils have limited amount of phosphorus attachment locations How much depends on soil characteristics Heger Wastewater 101 9
From: Urine and food breakdown Household cleaners and chemicals Impacts: Drinking water quality, weed and algal growth in costal environments Nitrogen Nitrogen Nitrogen starts as ammonia In an aerobic environment it is transformed to Nitrate during the nitrification process Nitrate generally moves with water Advanced treatment systems can be used to reduce nitrate if they return to an anaerobic environment in the denitrification process Biological Nitrification Biological Nitrification Organically bound nitrogen is released when the organic compound is oxidized released as the ammonium cation (NH4+) Nitrification is a two-step autotrophic process the conversion from ammonium to nitrate Nitrosomonas Step 1:NH 4+ + 3/2O 2 NO 2-2 + 2H + + H 2 O Nitrobacter Step 2:NO 2- + 1/2O 2 NO 3- During this energy yielding reaction some of the NH 4+ is synthesized into cell tissue giving the following overall oxidation and synthesis reaction: Autotrophic 1.00NH + + 1.89O 2 + 0.08CO 2 0.98NO - 3 + 0.016C 5 H 7 O 2 N + 0.95H 2 O + 1.98H + Bacteria new bacterial cells Nitrifiers use CO 2 instead of organic carbon as their carbon source for cell synthesis and for the conversion of NH 4+ to NO 3- -N. Total Nitrogen Measurements TKN+ Nitrate = Total N TKN = Organic N + NH 4 + Organic N found in cells of all living things (proteins, peptides, amino acids) principle compound in feces and urine 70% in urine not available to plants until bacterial conversion to inorganic form Ammonium N (NH 4+ ) immediately available for plant uptake positively charged, binds to soil particles converted to NO 3- in aerobic conditions Nitrate N (NO 3- ) immediately available for plant uptake stable over a wide range of conditions negatively charged, not held by soil high potential for leaching to groundwater converted to N 2 in anaerobic conditions volatilizes to atmosphere as NH 3 Heger Wastewater 101 10
Chemicals What? Cleaners Medications Impacts? Aquatic food chain, species reproduction, drinking water quality How treated? Stored in tank until pumped which can cause toxic affects Often removed in septic tank and soil treatment system More on this in presentation tomorrow Presentation Summary WASTEWATER WHAT IS IT AND HOW DO WE TREAT IT? VARYING CONTAMINANTS POTENTIAL NEGATIVE AFFECTS LABORATORY MEASUREMENTS Questions septic.umn.edu Heger Wastewater 101 11