Embozene TANDEM (Microspheres for Embolization)

Embozene TANDEM (Microspheres for Embolization) * EmbozeneTANDEM is available outside of the US only APM0017 120614 Rev B 1

PRODUCT INFORMATION Embozene Color-Advanced Microspheres Precise size calibration Structural integrity and compressibility Stable suspension Biocompatibility 2

PRODUCT INFORMATION Embozene TANDEM Microspheres for Embolization combine the precision and ease of use customers have always appreciated in Embozene Microspheres with full integration of drugs*. Due to the design characteristics, Embozene TANDEM Microspheres may be loaded with up to 50 mg/ml microspheres: Doxorubicin-HCIfor local, controlled, sustained dose elution to targeted tumor sites such as hepatocellular carcinoma (HCC) after embolization. Irinotecan-HCIfor local, controlled, sustained dose elution to targeted tumor sites such as metastatic colorectal cancer (mcrc) after embolization. * Embozene TANDEM microspheres are indicated for embolization according to the IFU. They may be loaded with a drug to elute a local, controlled, sustained dose of said drug to targeted tumor sites after embolization. Loading of drug should be under a physician s direction, choice and responsibility, based on type and dose of drug most beneficial to the patient. 3

DRUG-LOADING AND RELEASING MECHANISM Ion-exchange mechanism [Dox] + Doxo- Irino- or or [Iri] + rubicin tecan DEB-TACE non-ionic CA drug releasing via ion exchange 4

DRUG-ELUTING MICROSPHERES Advantages Higher potential for standarized procedure versus ctace 1 ctace improves survival compared to BSC 2 Reduced systemic exposure to Doxorubicin versus ctace 3 Reduced liver toxicity versus ctace 4 Reduced drug-related side effects versus ctace 4 Higher objective response for more advanced disease 4 Higher degree of necrosis 5 and longer time-to-progression 6 versus TAE Reduced systemic exposure to Irinotecan, higher and prolonged tumor drug concentration, and higher necrosis versus IA and IV 7 Improved survival versus FOLFIRI 8 Limitation Survival benefit for treatment of HCC 9 1. Lewis 2012 2. Llovet 2002, Lo 2002, Cammà 2002, Llovet 2003. 3. Varela 2007, Hong 2006, Gupta 2011, van Mahlenstein 2011 4. Lammer 2010 5. Lewis 2006, Nicolini 2010 6. Malagari 2010 7. Rao 2012 8. Aliberti 2011 9. Lammer 2010 vs Dhanasekaran 2010

DRUG-ELUTING MICROSPHERES TREND TO SMALLER SIZE Advantages of small over large microspheres Better/deeper tumor penetration 1,3 Higher degree of necrosis 2 Improved drug coverage 3 next step: small, tightly size-calibrated drug eluting microsphere ( 100 µm) 1. Lee 2008, Bonomo 2010; 2. Pelage 2002, Namur 2010; 3. Dreher 2012.

large bandwidth calibrated particles close bandwidth calibrated particles wide size range close size range Embozene Slide from Prof. Orsi (Milan, Italy)

PRODUCT INFORMATION TANDEM Microspheres Reference Numbers: Size 2mL Syringe 3mL Syringe 40 ± 10 µm 10420-TS0 10430-TS0 75 ± 15 µm 10720-TS0 10730-TS0 100 ± 25 µm 11020-TS0 11030-TS0 Embozene TANDEM is not currently available in the US. To order Embozene TANDEM, please contact your local CeloNova representative, or visit www.celonova.com 8

Contrast agent strategy Use only non-ionic contrast agent Use 5 mlsof contrast per 1 ml of TANDEM microspheres: Type Iodixanol Iohexol Iopamidol Iopromide Brand Name(s) - Visipaque - Accupaque -Omnipaque - Ioparimo -Isovue -Solutrast - Ultravist 2 ml TANDEM syringe: 10 mlsof contrast agent 3 ml TANDEM syringe: 15 mlsof contrast agent Ioversol - Optiject -Optiray 9

DRUG-LOADING PROCEDURE 1. 2. 3. 4. Doxorubicin (powder); dilute with WFI to get 20 mg/ml Remove excess of transport solution Aspire Doxorubicin Shake solution (20 mg/ml) according to IFU (at least every 5 mins for the first 20 mins) Remove excess of transport solution Easier drug-loading procedure High drug-loading efficiency

DRUG-LOADING TIMES Loading of Doxorubicin Bead size Doxorubicin Concentration [mg/ml] Loading Time [min] Loaded Drug [%] Post-Loading Size Change [%] TANDEM 40 µm 50 60 98 ±2 5 TANDEM 75 µm 50 60 98 ±2 5 TANDEM 100 µm 50 60 98 ±2 5 Competitor (100-300 µm) 37.5 60 98 ± 3 20* Loading of Irinotecan * for 25 mg/ml Bead size Irinotecan Concentration [mg/ml] Loading Time [min] Loaded Drug [%] Post-Loading Size Change [%] TANDEM 40 µm 50 30 98 ±2 5 TANDEM 75 µm 50 30 98 ±2 5 TANDEM 100 µm 50 30 98 ±2 5 Competitor (100-300 µm) 50 120 98 ± 3 30 Competitor (70-150 µm) 50 120 98 ± 3 30 Tables represent typical measured values, not specifications 11

TANDEM : TIGHT CALIBRATION AND SIZE STABILITY Microsphere Doxorubicin [mg/ml microspheres] Irinotecan [mg/ml microspheres] Embozene TANDEM 50 50 Competitor (100-300 µm) 37.5 50 Competitor (70-150 µm) - 50 Competitor(100 300 µm) Competitor (70 150 µm) Embozene TANDEM Relative particle count 3% 2% 1% 0% unloaded doxorubicin irinotecan 0 50 100 150 200 250 300 350 4% 3% 2% 1% 0% unloaded irinotecan 0 25 50 75 100 125 150 175 200 8% 6% 4% 2% 0% 40 µm 75 µm 100 µm unloaded doxorubicin irinotecan 0 25 50 75 100 125 150 175 200 Size [µm] Size [µm] Size [µm] Figures represent typical measured values, not specifications European Oncology & Haematology, 2012 (in press) 12

SIZE STABILITY MATTERS Model assumptions Packing density identical for unloaded and drug-loaded microspheres All microspheres have uniform size Total volume reduction due to size decrease during drug-loading Volume [%] 100 90 80 70 60 50 40 30 20 10 0 3 ml 4 ml 5 ml 6 ml 0 5 10 15 20 25 30 35 40 Size decrease [%] Embozene TANDEM Unstable microspheres Theoretical calculation

NUMBER OF MICROSPHERES Number of microspheres per ml in correlation to their size Number of microspheres [10 6 ] 50 40 30 20 10 Microsphere Microspheres per ml [10 6 ] Embozene TANDEM 40 µm 21 Embozene TANDEM 75 µm 4 Embozene TANDEM 100 µm 1.7 Competitor (100-300 µm) 0.1* 0 0 20 40 60 80 100 120 140 Microsphere size [µm] Competitor (70-150 µm) 0.65* *from competitors homepage Packing density 70% Typical measured values, not specifications

DRUG-RELEASE TIMES Release of Irinotecan 75% Embozene TANDEM (40 µm) Embozene TANDEM (75 µm) Embozene TANDEM (100 µm) Competitor (70-150 µm) Competitor (100 300 µm) Microsphere Embozene TANDEM (40 µm) Embozene TANDEM (75 µm) Embozene TANDEM (100 µm) Competitor (100-300 µm) Competitor (70-150 µm) t 75% [min] 67 49 60 9 9 Release profiles of Irinotecan-loaded DEB (50 mg/ml microspheres); release monitored in process via UV-VIS spectroscopy at 37 C in SOTAX CE-1 elution system using isotonic medium, 5 ml/min flow rate. Typical measured values, not specifications European Oncology & Haematology, 2012 (in press) 15

DRUG-RELEASE TIMES Release of Irinotecan Bead size Irinotecan Concentration [mg/ml] Time to release 25 mg Irinotecan[h] TANDEM 40 µm 49 ±1 1 TANDEM 75 µm 49 ±1 0.5 TANDEM 100 µm 49 ±1 0.5 Competitor (100-300 µm) 49 ± 1.5 0.1 Competitor (70-150 µm) 49 ± 1.5 0.1 Typical measured values, not specifications 16

DRUG-RELEASE TIMES Release of Doxorubicin Bead size Doxorubicin Concentration [mg/ml] Time to Release 10 mg Doxorubicin [h] TANDEM 40 µm 49 ±1 3 TANDEM 75 µm 49 ±1 3 TANDEM 100 µm 49 ±1 3 Competitor (100-300 µm) 36.5 ± 1.5 1.5 Competitor (500-700 µm) 36.5 ± 1.5 8.5 Small drug-eluting microspheres cause higher degree of necrosis 1,2,4 Large drug-eluting microspheres release slower 1,3 Large drug-eluting microspheres cause lower systemic drug levels 1 Large drug-eluting microspheres cause higher drug tissue concentration 4 Embozene TANDEM combines benefits of small microspheres with slower drug release. Typical measured values, not specifications 1. Lewis 2006; 2. Nicolini 2010; 3. Gonzales 2008, Taylor 2008, Tang 2008; 4. Namur 2010 17

TANDEM PRICING Because of the higher drug loading capacity and the offering of 3 ml syringes, TANDEM will be more cost efficientfor the hospital. Example: a patient needs a dose of 150 mg of Doxorubicin -TANDEM loads the drug faster, saving time and money -TANDEM is capable of delivering 150 mg with one 3 ml syringe -TANDEM can load 33% more drug in a 2 ml syringe Competition TANDEM Maximum drug loading capacity per 2 ml vial or syringe 75 mg 100 mg Maximum drug loading capacity per 3 ml vial or syringe N/A 150 mg Maximum drug loading capacity per 4 ml vial or syringe 150 mg (need 2 x 2 ml vials) N/A 18

TANDEM KEY FEATURES Can load doxorubicin and irinotecan faster and easier Save time for the pharmacy (up to 90 minutes for loading irinotecan) Can load more drugs: up to 50 mg/ml microspheres Load 150 mg of drug in one 3 ml syringe Drugs release more slowly May reduce systemic side effects Microspheres do not change in size after drug loading Easy passage through microcatheters Ideal for targeted drug delivery near the tumor site 19

VALUE PROPOSITION TANDEM Microspheres are cost efficient and beneficial for: The Hospital: Capable of delivering drugs up to 150 mg with one 3 ml syringe Capable of delivering more drugs with a 2 ml syringe The Pharmacy: Easier drug loading, which saves time (up to 90 minutes faster for irinotecan and up to 60 minutes faster for doxorubicin) The Physician: Choice between 2 ml and 3 ml volumes (no need to use multiple vials or syringes during the same procedure) Ease of use (better fit through microcatheters) Targeted embolizations (Tight calibration, less size changes after drug loading) The Patient TANDEM Microspheres have a slower and more controlled drug release, which may result in fewer systemic effects and providing a higher level of patient care 20

TANDEM POSITIONING Precision Targeted drug delivery with tightly calibrated microspheres and superselective TACE Controlled and precise drug delivery and release at tumor site Microsphere size remains stable during drug loading and storage: typical size change less than 5% 21

TANDEM: TIGHT CALIBRATION AND SIZE STABILITY TANDEM 40 µm TANDEM 75 µm TANDEM 100 µm Competitor (100 300 µm) Tight size-calibration decreases risk of premature vessel occlusion Scale bar: 100 µm 22

TANDEM POSITIONING Efficiency Deliver up to 150 mg of Doxorubicin-HClor Irinotecan-HClwith one 3 ml syringe Fast drug loading times with superior drug loading capacity up to 50 mg/ml microspheres Controlled drug release rates for optimal localized drug delivery Flexibility Available in three sizes (40 µm, 75 µm and 100 µm) Available in two volumes (2 ml & 3 ml syringes) Ideal for passage through micro catheters 23

TANDEM POSITIONING Safety Color coded labels for easy recognition of microsphere sizes Slow and controlled drug release times may result in fewer systemic side effects 24

SUMMARY Embozene TANDEM offers Small sizes Tight size calibration Size stability Fast drug-loading with high loading capacity Slow and controlled drug release Embozene TANDEM is designed to Enable super-selective embolization Penetrate deeper into the tumor micro-vasculature Reduce systemic toxicity Improve drug coverage Increase tumor kill