Township Of Georgian Bay. Inland Water Quality Program Report. Paul M. Wiancko - B. Sc., M. Eng

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Township Of Georgian Bay Inland Water Quality Program Report 2015 By Paul M. Wiancko - B. Sc., M. Eng 1

Table of Contents Page Executive Summary 3 1.0 Introduction 5 2.0 Water Quality Analyses 6 2.1 Results 6 2.1.1 Severn River 6 2.1.2 Gloucester Pool 7 2.1.3 Six Mile Lake 8 2.1.4 Gibson Lake 9 2.1.5 Go Home Lake 10 2.1.6 Galla Lake 10 2.1.7 Stewart Lake 11 3.0 Bacteria Results 12 4.0 Recommendations 13 Appendix 1 14 Appendix 2 16 Appendix 3 23 Appendix 4 25 2

Executive Summary The Township of Georgian Bay Inland Water Quality Program has been ongoing for 18 years with the beginning of bacteria sampling in 1997. In 2005, the program was expanded to include water chemistry based on a similar program underway on Georgian Bay. The results of all inland water studies were summarized in a report to the Township in 2010 entitled Water Quality Monitoring Report, Inland Lakes Township of Georgian Bay, Summary 2005 2010. Annual reports were produced from 2011 to 2015. During the spring of 2011, talks were held with the District of Muskoka Watershed Council, their environmental consultant and various lake associations to standardized sampling protocol across the District. This led to a number of significant changes to the program that now allow for a direct comparison of Township data to District wide data. Changes included sampling protocol and analytical services (Table 1). In 2015, a total of seven inland lakes were sampled as part of a Township wide volunteer program for water quality and bacterium. The inland lakes sampled were Severn River (above the Big Chute), Gloucester Pool, Baxter Lake, Six Mile Lake, Gibson Lake, Go Home Lake and Stewart Lake. Water quality results for 2015 were compared to a summary of long term means from 2005 2014. New to the report this year are the 2002 2014 Ministry of the Environment and Climate Change, Lake Partnership Program total phosphorous data (Appendix 3). The Severn River had a higher level of clarity in 2015 (5-7 m.) when compared to the long term 2006-2014 mean (4.4 m.). This increased clarity was noticed in most on the inland lakes in 2015. The 2015 Severn River levels of TP (5.7 10.3 µg/l) are below the long term mean (14.0 µg/l) with Lost Channel always being the lowest value (5.7 µg/l). Never the less, the Severn River continues to have the highest TP values recorded within the Inland Lake Water Quality Program. The source water for the Severn River is Lakes Simcoe and Couchiching. The Severn River waters feed both Gloucester Pool and Six Mile Lake. Gloucester Pool TP levels in 2015 (10.1 14.6 µg/l with a mean of 11.3 µg/l) were every similar to the 2005 2014 to long term mean of 11.1 µg/l. The discharges from Gloucester Pool to Georgian Bay via Little Lake (11.7 µg/l) and Baxter Lake (11.7 µg/l) which enters South Bay are basically similar to the inflow at the Big Chute (10.9 µg/l). 3

Six Mile Lake receives all of its water from the Severn River above the Big Chute (Copp Bay area) where the 2015 TP mean was 10.2 µg/l This value is slightly higher compared to the SML inflow at Lost Channel (8.7 µg/l). Overall, the mean for TP in SML in 2015 was 8.4 µg/l which is comparable to its long term mean of 9.0 µg/l and the District Watershed value of 8.3 8.8 µg/l. TP values in Gibson Lake remained in the 9.0 11.1 µg/l range. The 2015 mean was 10.1 µg/l which is very similar to the long term mean of 12.2 µg/l. This long term mean is in line with the District Watershed mean of 10.1 11.8 µg/l. TP bottom sample values varied slightly (17.0 39.4 µg/l) from the long term trend (27.9 µg/l). In Go Home Lake, TP values varied from a low of 6.1 µg/l at the Control Dam to a high of 11.5 µg/l in Four Seasons Bay. However, overall the 2015 mean TP of 8.4 µg/l was lower then the long term mean of 9.9 µg/l. The overall Districts Watershed TP mean for Go Home Lake is 6.7 µg/l. In Galla Lake, TP throughout the lake was very similar (9.0 11.1 µg/l) but higher then the previous long term (2006 2012) mean of 7.6 µg/l. For Stewart Lake, TP was consistent across the lake (4.0 4.5 µg/l) and down from the 2010 2014 mean of 7.3 µg/l. The District Watershed long term mean is 7.9 µg/l. The bottom waters in Kilty Bay was way down in 2015 (7.9 µg/l) from 2014 (23.1 µg/l). For bacteria, all five sample dates on the Severn River from 11 sites between June29 and August 24 were way below any of the criteria. E coli in all samples were <3 EC/100 mls. Total bacteria in Gloucester Pool, Six Mile Lake, Gibson Lake and Stewart Lake were all in the acceptable range with only a few E coli samples above 10 EC/100 ml but well below the 100 EC/100 ml limit. Six Mile Lake had two samples at 43 EC/100 ml on June 15. Go Home Lake had a number of Provincial exceedences of Total Coliforms starting on July 4 in Pike Bay through to August 29 at all six sample locations. Go Home Lake Marina had 3 sample dates above the Provincial criteria. E coli results were below the Inland Lakes Objective of 10 EC/100 ml except for 5 out of 6 sample dates near the Go Home Lake Marina. Exceedences varied from 11 43 ED/100 ml 4

1.0 Introduction In 2015, a total of seven inland lakes were sampled as part of a Township wide volunteer program for water quality and bacteria. The inland lakes sampled were Severn River (above the Big Chute), Gloucester Pool, Baxter Lake, Six Mile Lake, Gibson Lake, Go Home Lake and Stewart Lake. Water quality analysis included vertical temperature and oxygen profiles (top to bottom at every meter), conductivity and total phosphorous at the surface and below the thermocline (1 meter off the bottom) and water clarity at the surface. In early spring, lake water temperatures, oxygen levels and chemistry tend to be uniform from top to bottom. As the surface waters warm up in late spring and summer, the lakes stratify whereby a thermocline is formed at about the 5-8 meter depth. A thermocline is a horizontal layer of water about 3 meters wide where temperature and oxygen levels suddenly drop. This thermocline prevents the mixing of the top and bottom water columns. It is also a barrier to vertical fish movement and chemical mixing. Total Phosphorous (TP) above the thermocline tends to be a result of local surface runoff (wetlands, septic systems, storm runoff, etc.), while TP below the thermocline tends to be dominated by the release from bottom sediments. This TP release from the bottom happens when oxygen levels are <1 mg/l (anoxic conditions). TP levels < 10 µg/l are considered good, 10-20 µg/l moderate and > 20 µg/l of concern. New to this years report are the 2002 2014 Ministry of the Environment and Climate Change, Lake Partnership Program total phosphorous data (Appendix 3). These samples are typically take at the surface during spring or early summer and are analyzed at the Dorset Lab. Conductivity is used as an indicator of water movement and mixing throughout the lake. A thermocline prevents this mixing and thus conductivity can be used to determine surface and bottom horizontal water movements. Clarity is measured to determine the turbidity of the surface waters. High turbidity is usually the result of high biological productivity (algae growth) which is a result of higher nutrient (TP) levels. It can also be caused by sediments from storm runoff. Bacteria samples were mostly collected on a bi-monthly basis from late June to early September. The ColiPlate test kit was used to analyze all water samples for Total Coliform and Fecal Coliform (E. coli). This method has been approved by the American Public Health Association and the Environmental Protection Agency. Water samples are incubated for 24-26 hours. After incubation, individual cells in the microplate turn blue in the presence of Total Coliform indicating contamination from runoff. If cells exhibit fluorescence under a UV light then fecal E. coli is present. The Most Probable Number 5

technique is used to convert the positive well counts to cell density in cfu s/100ml sample. The criteria are: Total Coliform (Provincial) 1000 TC/100 ml sample E. coli (Provincial) 100 EC/100 ml sample E. coli (Georgian Bay and Inland Lakes Objective) 10 EC/100 ml samples 2.0 Water Quality Analyses The 2015 field results for the seven inland lakes are outlined in Tables 2-8. Maps of all the sample locations for all lakes except Galla Lake are outlined in Appendix 4. The 2015 data is compared to the mean results collected over 2005 2014 as summarized in the Township report Water Quality Monitoring Report, Inland Lakes Township of Georgian Bay, Summary 2005 2010 and the 2011, 2012, 2013 and 2014 annual reports, all available on the Township web site. In 2011, due to valued input from the District Watershed Council and their consultant - Hutchison Environmental, critical changers were made to the present program as summarized in Table 1. The results of these changes will allow for a direct comparison to the 2011 to 2013 results but may not necessarily allow for a direct comparison to the 2005-2010 results. Table 1 Sample and Analytical Differences Between Previous Years 2005-2010 and the Present Sampling Program (2011 2015) Parameter 2005-2010 Methodology Present Methodology Sample filtration Not filtered Filtered through 80 µ mesh TP Laboratory Used Maxxium Analytical Dorset Trent University Collection of bottom samples for TP 2.1 Results Collected 2-3 meters below thermocline Collected 1 meter off the bottom. The 2015 results are presented in tabular form and compared to the mean of results from 2005-2014. All six lakes were sampled from Sept 7-12, 2015. 2.1.1 Severn River Four sites were sampled on the Severn River Russian Bay, Wood Bay, Lost Channel and Copp Bay. The results and summarized in Table 2. Conductivity seems to be consistent year most years with a little higher values in 2015, but Lost Channel still shows an additional source of water. This additional source tends to 6

Water Quality Indicators T Bottom reduce the level of TP each year ( 5.7 µg/l in 2015) in comparison to the other bays sampled (8.9 14.0 µg/l). The Severn River had a higher level of clarity in 2015 (5-7 m.) when compared to the long term 2006-2014 mean (4.4 m.). The 2015 levels of TP (5.7 10.3 µg/l) are below the long term mean (14.0 µg/l) with Lost Channel always being the lowest value (5.7 µg/l). Never the less, the Severn River continues to have the highest TP values recorded within the Inland Lake Water Quality Program. The source water for the Severn River is Lakes Simcoe and Couchiching. The Severn River waters feed both Gloucester Pool and Six Mile Lake. There was a thermocline measured in Lost Channel this year with a slight rise to 16.8 µg/l at the bottom compared to the surface of 5.7 µg/l. 2.1.2 Gloucester Pool Mean 2006-2014 Table 2 Severn River 2015 Water Quality Results Russian Bay Wood Bay Lost Channel Copp Bay Nine sites continued to be sampled on Gloucester Pool Big Chute Bay, Six Mile Channel, White Falls Bay, Main Pool, Little Go Home Bay, Black River Channel, Upper Little Lake, Lower Little Lake and Baxter Lake. The results are outlined in Table 3. As in previous years, conductivity in Big Chute Bay (336.8 µs/cm) reflects the upstream Severn River value in Copps Bay (347.4 µs/cm). As additional sources of water are added to Gloucester Pool from Six Mile Lake via Six Mile Channel and White Falls Bay and from MacLean Lake via Black River Channel, conductivity values are slightly reduced downstream in Little Lake (305.8 301.5 µs/cm). Clarity was slightly improved at most sites this year (> 5 m.) compared to the 2005 2014 mean of 4.8 m. Mean 2015 Conductivity T 276.2 341.0 346.4 304.8 347.4 334.9 (µs/cm) B 208.3 Secchi Disk 4.4 5 7 5 5.5 5.6 (meters) Total Phosphorous (µg/l) T 14.0 10.3 9.5 5.7 10.2 8.9 B 16.8 7

Water Quality Indicators T Bottom Mean 2005-2014 Big Chute Bay Table 3 Gloucester Pool 2015 Water Quality Results Six Mile Channel White Falls Bay Main Pool Little Go Home Bay Black Rive Channel Upper Little Lake Lower Little Lake Baxter Lake Conductivity T 237.5 336.8 209.5 249.7 304.6 284.6 300.4 305.8 301.5 252.4 282.8 (µs/cm) B 201.3 216.5 Secchi Disk 4.8 5.5 B 5 5.2 5.9 B 3.8 B 5.1 5.1 (meters) Total T 11.1 10.9 10.9 10.1 10.1 10.1 12.1 14.6 11.7 11.7 11.3 Phosphorous B 23.4 21.4 (µg/l MOEC TP 10.6 9.5 10.2 2002, 2005 & 2011-12 9.9 9.7 10.3 MOEC TP Ministry of the Environment & Climate Change Total Phosphorous Gloucester Pool TP levels in 2015 (10.1 14.6 µg/l with a mean of 11.3 µg/l) were every similar to the 2005 2014 to long term mean of 11.1 µg/l. The discharges from Gloucester Pool to Georgian Bay via Little Lake (11.7 µg/l) and Baxter Lake (11.7 µg/l) which enters South Bay are basically similar to the inflow at the Big Chute (10.9 µg/l). 2.1.3 Six Mile Lake Six sites were sampled in Six Mile Lake (SML) Lost Channel, Main Lake, Crooked Bay, Long Lake, Hungry River and TransCanada Bay. Results are outlined in Table 4. Water Quality Indicators Conductivity T Bottom Mean 2005-2014 Table 4 Six Mile Lake 2015 Water Quality Results Lost Channel Main Lake Crooked Bay Long Lake Hungry River TransCanada Bay T 197.3 212.7 172.6 136.0 115.4 155.3 155.4 157.9 (µs/cm) B 128.4 133.9 135.0 142.3 107.6 191.1 142.0 Secchi Disk 5.5 5.5 5.5 5.5 3.5 B 5.5 5.1 (meters) Total T 9.0 8.7 5.1 7.5 7.9 11.8 9.6 8.4 Phosphorous B 13.3 20.8 7.9 10.9 39.4 19.8 (µg/l MOE-TP 6.8 9.9 9.0 2002-2014 7.2 9.8 6.8 MOEC TP Ministry of the Environment & Climate Change Total Phosphorous SML tends to stratify into five separate basins over the summer months based on conductivity. Lost Channel and the Main Lake are usually very similar but for the second year in a row there is a slight different (212.7 µs/cm compared to 172.6 µs/cm respectively). The source water for Six Mile Channel (Gloucester Pool) of 209.4µS/cm is from the Lost Channel (SML) area with a value of 212.7 µs/cm. Clarity, as measured via secchi disk readings, was very similar to the long term average. Mean 2015 Mean 2015 8

Six Mile Lake receives all of its water from the Severn River above the Big Chute (Copp Bay area) where the 2015 TP mean was 10.2 µg/l This value is slightly higher compared to the SML inflow at Lost Channel (8.7 µg/l). Overall, the mean for TP in SML in 2015 was 8.4 µg/l which is comparable to its long term mean of 9.0 µg/l and the District Watershed value of 8.3 8.8 µg/l (Appendix 2). 2.1.4 Gibson Lake Sampling continued at three sites on Gibson Lake South Lake, Middle Lake and North Lake (Table 5). The conductivity mean (40.3 µs/cm) was slightly higher this year compared to the 2006 2014 mean of 32.7 µs/cm. Gibson Lake, which receives its source water from wetlands to the south, contains a high level of tannins which colour its water and reduces its clarity. In 2015, clarity was slightly up (2.8 m.) compared to the long term mean of 2.4 m. The tannins can also result in the reduction of oxygen levels below the thermocline at around the 5 meter depth. While historical oxygen levels below the thermocline can reach < 2 mg/l, for a third year in a row, 2015 levels remain higher at between 4.2 4.9 mg/l. Fish require levels above 3 mg/l to survive. TP values in Gibson Lake remained in the 9.0 11.1 µg/l range. The 2015 mean was 10.1 µg/l which is very similar to the long term mean of 12.2 µg/l. This long term mean is in line with the District Watershed mean of 10.1 11.8 µg/l (Appendix 2). TP bottom sample values varied slightly (17.0 39.4 µg/l) from the long term trend (27.9 µg/l). Water Quality Indicators Conductivity T Bottom Table 5 Gibson Lake 2015 Water Quality Results Mean 2006-2014 South Lake Middle Lake North Lake Mean 2015 T 32.7 38.6 39.0 43.3 40.3 (µs/cm) B 35.6 25.6 25.8 45.4 32.2 Secchi Disk 2.4 3 3 2.5 2.8 (meters) Total T 12.2 10.3 9.0 11.1 10.1 Phosphorous B 27.9 21.4 17.0 39.4 25.9 (µg/l MOEC - TP T1 11.2 12.9 11.8 11.9 2003 & T2 10.0 13.0 10.0 11.0 2011-2014 MOEC TP Ministry of the Environment & Climate Change Total Phosphorous 9

2.1.5 Go Home Lake Seven locations were sampled in Go Home Lake Control Dam, Blue Lagoon, Four Seasons Bay, Bay of Many Winds, Crystal Bay, Swallow Bay, and Manning Bay. Conductivity has been very consistence over the years as seen in Table 6 and from the inlet at Swallow Bay (54.0 µs/cm) to the outlet at the Control Dam (52.8 µs/cm). Manning Bay, at 40.9 µs/cm, receives additional flows from Irving Lake. Water Quality Indicators T Bottom Mean 2005-2014 Table 6 Go Home Lake 2015 Water Quality Results Control Dam Blue Lagoon Four Seasons Bay Bay of Many Winds Crystal Bay Swallow Bay Manning Bay Conductivity T 49.8 52.8 52.8 52.3 53.0 53.1 54.0 40.9 51.3 (µs/cm) B 35.2 44.5 Secchi Disk 4.8 4.6 4.2 5.2 4 4.3 3.6 4.1 4.3 (meters) Total T 9.9 6.1 5.6 11.5 7.7 9.4 8.9 9.6 8.4 Phosphorous B 15.5 7.1 24.8 (µg/l) MOEC-TP 6.5 6.2 2002-2014 6.1 6.9 MOEC TP Ministry of the Environment & Climate Change Total Phosphorous Mean 2015 Clarity ranged from a high of 5.2 m. in Four Seasons Bay to a low of 3.6 m. in Swallow Bay for an across lake mean of 4.3 m. This is similar to the long term mean of 4.8 m. TP values varied from a low of 6.1 µg/l at the Control Dam to a high of 11.5 µg/l in Four Seasons Bay. However, overall, the 2015 mean TP of 8.4 µg/l was lower then the long term mean of 9.9 µg/l. The overall Districts Watershed TP mean for Go Home Lake is 6.7 µg/l (Appendix 2). Manning Bay s bottom waters (24.8µg/L) continues to be comparable to 23 µg/l in 2005, ten years ago. 2.1.6 Galla Lake Galla Lake rejoined the inland program this year after a two year absents. Samples were collected at three sites East Bay, Lot 7 and Outfall Bay. Conductivity continues to remain similar (21.9 µs/cm) from the 2006 2012 program. Clarity has not changed over the years. Like Gibson Lake, Galla Lake contains tannins which reduce clarity. 10

Water Quality Indicators Conductivity T Bottom Table 7 Galla Lake 2015 Water Quality Results (no samples on 2013 & 2014) Mean 2006-2012 East Bay Lot 7 Outlet Bay Mean 2015 TP throughout the lake was very similar (9.0 11.1 µg/l) but higher then the previous 2006 2012 long term mean of 7.6 µg/l. East Bay is the inlet area receiving its waters from wetlands. 2.1.7 Stewart Lake Stewart Lake was sampled at four locations in September Kilty Bay, Big Bay, Bear Bay and Stewart Bay (Table 7). Conductivity was very consistent across the lake (131.6 132.9 µs/cm) in 2015 and in line with the long term mean of 135.2 µs/cm. Water Quality Indicators Conductivity Top Bottom Table 8 Stewart Lake 2014 Water Quality results T 24.4 21.8 21.9 21.9 21.9 (µs/cm) B 35.0 11.8 Secchi Disk 3.2 3 3 3 3 (meters) Total T 7.6 11.1 9.0 10.3 10.1 Phosphorous B 39.4 17.0 (µg/l MOEC-TP 7.5 2003, 2009-7.5 2011 MOEC TP Ministry of the Environment & Climate Change Total Phosphorous 2010-2012 2014 Kilty Bay Big Bay Bear Bay Stewart Bay 2015 Mean T 135.2 131.6 131.8 132.2 132.9 132.1 (µs/cm) B 106.2 112.6 Secchi Disk 3.6 4 4 4.5 4 4.1 (meters) Total T 7.3 5.4 4.8 6.3 4.8 5.3 Phosphorous (µg/l) B 7.9 9.4 MOEC-TP 8.7 2003-2004 2009-2014 7.2 MOEC TP Ministry of the Environment & Climate Change Total Phosphorous 11

Clarity mean was up slightly (4.1 m.) from the 2010 2014 mean of 3.6 m. TP was consistent across the lake (4.0 4.5 µg/l) and down from the 2010 2014 mean of 7.3 µg/l. The District Watershed long term mean is 7.9 µg/l. The bottom waters in Kilty Bay was way down in 2015 (7.9 µg/l) from 2014 (23.1 µg/l). The bottom waters in Big Bay is similar to 2011 (7.1 µg/l) and 2012 (14.8 µg/l). 3.0 Bacteria Results As mentioned in the introduction, bacteria samples were collected on mostly a bi-monthly basis from late June to early September. The ColiPlate test kit was used to analyze all water samples for Total Coliform and fecal coliform (E. coli). This method has been approved by the American Public Health Association and the Environmental Protection Agency. Water samples are incubated for 24-26 hours. After incubation, individual cells in the microplate turn blue in the presence of Total Coliform indicating contamination from runoff. If cells exhibit fluorescence under a UV light then fecal E. coli is present. The Most Probable Number technique is used to convert the positive well counts to cell density in cfu s/100ml sample. The criteria are: Total Coliform (Provincial) 1000 TC/100 ml sample E. coli (Provincial) 100 EC/100 ml sample E. coli (Georgian Bay and Inland Lakes objective) 10 EC/100 ml sample The 2015 bacteria results are outlined in Appendix 1 Severn River All five sample dates from the 11 sites between June 29 and August 24 were way below any of the criteria. E coli in all samples were <3 EC/100 mls. Gloucester Pool, Six Mile Lake, Gibson Lake and Stewart Lake Total bacteria were all in the acceptable range with only a few E coli samples above 10 EC/100 ml but well below the Provincial 100 EC/100 ml limit. Six Mile Lake had two samples at 43 EC/100 ml on June 15. Go Home Lake Go Home Lake had a number of Provincial exceedences of Total Coliforms starting on July 4 in Pike Bay through to August 29 at all six sample locations. Go Home Lake Marina had 3 sample dates above the Provincial criteria. E coli results were below the Inland Lakes Objective of 10 EC/100 ml except for 5 out of 6 sample dates near the Go Home Lake Marina. Exceedences varied from 11 43 ED/100 ml 12

Graph 1 E Coli Results 2015 12 10 E Coli per 100 ml 8 6 4 2 0 Severn River Gloucester Pool Six Mile Lake Gibson Lake Go Home Lake Gall Lake Stewart Lake Lake 4.0 Recommendations There were no anomalies in the data for water chemistry in 2015, Therefore there is no additional work or changes recommended to this program for 2016. Based on a series of historical high E coli readings in Go Home Lake from 1997-2015, it is still recommended that we work with Georgian Bay Forever to conduct a DNA assessment at several sample sites. 13

Appendix 1 Total Coliform and E coli Monitoring Results for Inland Lakes 2015 2015 Sample Locations Copp Bay Coulters Bay Lost Channel Wood Bay Severn Falls Cherry Creek MPN TOTAL COLIFORMS per 100ml sample MPN E COLI per 100ml sample Sampling Dates Sampling Dates Jun-29 Jul-13 Jul-27 Aug-08 Aug-24 Jun-29 Jul-13 Jul-27 Aug-08 Aug-24 42 13 36 15 47 0 0 0 0 3 28 12 39 31 35 1 0 1 1 0 N/A 4 15 33 9 N/A 0 0 2 2 28 24 16 10 20 0 0 0 0 0 36 20 31 22 28 0 0 1 1 3 90 50 37 N/A 27 2 2 1 N/A 1 36 81 20 N/A 22 4 0 2 N/A 2 Hungarian Bay Russian Bay 40 26 13 N/A 20 2 0 0 N/A 1 Control Site 44 25 17 N/A 21 1 0 0 N/A 1 Pretty Channel 24 4 37 N/A 96 0 0 0 N/A 0 Big Chute 47 12 32 N/a 40 0 2 0 N/A 5 Aver. Lake Temp F (10 locations) BACTERIOLOGICAL WATER QUALITY MONITORING for Severn River (SRAPO) 69 75 76 75 74 2015 BACTERIOLOGICAL WATER QUALITY MONITORING for Gloucester Pool MPN TOTAL COLIFORMS per 100ml sample MPN E COLI per 100ml sample Sampling Dates Sampling Dates Sample Locations June 19th September 10th June 19th September 10th Six Mile Channel 3 8 0 3 Big Chute 0 28 0 0 Whites Falls 0 3 0 0 Gloucester Pool Center 3 8 0 0 Little Go Home Bay 8 11 0 3 ` Black River 33 16 8 0 Narrows 33 5 11 0 Little Lake Center Lock 45 Dam G Aver. Lake Temp F (10 locations) 3 5 0 0 65 3 11 0 36 22 3 5 21c/70f 23c/73.4f BACTERIALOGICAL WATER QUALITY MONITORING for SIX MILE LAKE 2015 Sample Locations East Crooked Bay (w/side Riddy's) Grand Island (N of center cut) Wawautosa Bay (N half) East Hungry Bay (river mouth) Battleship Island (east side) Barefoot Bay (mouth) Whites Bay (middle) Whites Falls (narrows B4 dam) Six Mile Marina (middle of bay) Prov.Park (south of beach) Aver. Lake Temp F (10 locations) MPN TOTAL COLIFORMS per 100ml sample MPN E COLI per 100ml sample Sampling Dates Sampling Dates June 15 July 11 July 26 Aug 10 Aug 27 Sept 14 June 15 July 11 July 26 Aug 10 Aug 27 Sept 14 146 46 13 13 30 19 11 8 3 3 3 3 49 36 11 52 22 5 11 3 3 3 3 3 188 65 52 13 22 13 43 5 3 3 8 3 76 156 69 136 90 43 3 11 3 3 3 3 43 16 22 49 28 43 3 8 3 3 3 3 247 36 62 46 59 11 43 3 3 8 3 3 79 46 25 83 22 25 3 3 3 3 3 3 76 106 30 83 79 79 8 16 3 3 3 3 94 39 33 98 151 49 25 3 3 3 3 3 146 83 16 55 119 43 19 19 3 3 3 3 74.3 78.1 77.1 75.7 71.8 69.5 2015 Sample Locations South Bay Landing Middle Island Wahta North River BACTERIOLOGICAL WATER QUALITY MONITORING for Gibson Lake MPN TOTAL COLIFORMS per 100ml sample MPN E COLI per 100ml sample Sampling Dates Sampling Dates July 3 July 20 Aug 6 Aug 23 Sept 9 July 3 July 20 Aug 6 Aug 23 Sept 9 65 8 22 52 22 0 5 8 0 3 36 19 19 35 19 5 8 3 13 8 13 22 22 33 22 0 5 3 3 5 8 16 22 22 22 3 11 3 8 5 13 13 36 110 36 8 3 3 3 3 5 11 123 136 136 5 8 0 5 3 Aver. Lake Temp F (10 locations) 72 75 73 73 76 14

2015 Sample Locations Four Seasons Bay Bay of Many Wiinds Bay of Many Winds Campsite Heart Bay Go Home Lake Marina Pike Bay BACTERIOLOGICAL WATER QUALITY MONITORING for Go Home Lake MPN TOTAL COLIFORMS per 100ml sample MPN E COLI per 100ml sample Sampling Dates Sampling Dates 20-Jun 04-Jul 18-Jul 01-Aug 15-Aug 29-Aug 12-Sep 20-Jun 04-Jul 18-Jul 01-Aug 15-Aug 29-Aug 12-Sep 52 22 16 25 1696 166 171 0 0 0 0 25 0 8 59 13 30 2424 2424 298 161 3 0 11 0 3 3 3 8 11 25 2424 2424 194 141 0 0 5 3 0 0 8 8 49 16 25 102 2424 166 3 0 0 3 0 3 0 83 90 2424 2424 2424 451 298 25 43 8 19 25 11 8 22 2424 30 43 33 2424 72 0 0 0 0 0 0 0 Aver. Lake Temp C (6 locations) Not Recorded 22.2 23.2 25.4 24.3 22 23.2 BACTERIOLOGICAL WATER QUALITY MONITORING for Galla Lake 2015 Sample Locations East Bay By Lot #7 Boat Launch By Lot 29 Aver. Lake Temp F (10 locations) MPN TOTAL COLIFORMS per 100ml sample MPN E COLI per 100ml sample Sampling Dates Sampling Dates Jun 27 July 11 July 25 Aug 8 Aug 22 Sept 5 Jun 27 July 11 July 25 Aug 8 Aug 22 Sept 5 22 0 39 72 22 69 0 0 0 5 5 3 10 39 43 36 5 65 0 3 0 11 5 5 36 2424 19 28 39 49 0 5 0 0 8 0 83 240 39 65 156 119 0 5 3 19 11 11 2015 Sample Locations Kilty Bay Beaver Dam Boat Launch Town Storm Water Exit Various Near Taggart's Love's BACTERIOLOGICAL WATER QUALITY MONITORING for Stewart lake MPN TOTAL COLIFORMS per 100ml sample MPN E COLI per 100ml sample Sampling Dates Sampling Dates June 29 July 11 July 28 August 15 August 31 June 29 July 11 July 28 August 15 August 31 19 49 <3 110 19 5 16 <3 28 8 46 46 8 87 11 3 11 3 46 8 8 25 8 52 28 3 3 11 13 3 16 30 8 136 25 3 5 3 43 8 16 19 3 19 13 8 5 <3 5 <3 55 30 8 49 33 3 5 5 36 3 15

Appendix 2 District of Muskoka Watershed Reports 16

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Appendix 3 Ministry of the Environment and Climate Change Lake Partnership Program 2002 2014 Total phosphorous (TP) Concentration Data TP1 and TP2 are duplicate samples Gloucester Pool Latitude Longitude TP1 TP2 Lake Name Township STN Site ID Site Description (DMS) (DMS) Date (µg/l) (µg/l) GLOUCESTER POOL BAXTER 1666 1 Mid Lake, deep spot 445057 794202 09-Jun-02 9.0 8.4 GLOUCESTER POOL BAXTER 1666 2 Mid Lake, deep spot 445055 794205 10-Jul-05 12.2 11.3 GLOUCESTER POOL BAXTER 1666 3 SE Little GoHomeBay 445055 794213 21-May-11 10.0 9.8 GLOUCESTER POOL BAXTER 1666 3 SE Little GoHomeBay 445055 794213 13-May-12 9.0 9.6 Baxter Lake Latitude Longitude TP1 TP2 Lake Name Township STN Site ID Site Description (DMS) (DMS) Date (µg/l) (µg/l) BAXTER LAKE BAXTER 6929 1 Mid Lake, deep spot 445300 794500 02-Jun-04 10.2 10.3 Six Mile Lake Latitude Longitude TP1 TP2 Lake Name Township STN Site ID Site Description (DMS) (DMS) Date (µg/l) (µg/l) SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 25-May-02 9.0 12.0 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 20-May-03 7.8 8.0 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 30-May-03 7.4 8.6 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 24-May-04 9.4 8.8 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 05-May-05 12.2 10.4 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 23-May-06 7.7 9.3 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 21-May-07 6.6 6.8 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 05-May-08 10.1 9.4 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 15-May-09 10.0 9.2 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 03-May-10 9.8 12.0 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 23-May-11 9.8 8.4 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 13-May-12 11.4 10.8 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 27-May-13 9.6 14.6 SIX MILE LAKE BAXTER 4978 1 Cedar Nook 445458 794507 26-May-14 8.4 9.4 SIX MILE LAKE BAXTER 4978 4 Mid Lake, deep spot 445401 794242 30-May-03 6.8 7.2 SIX MILE LAKE BAXTER 4978 5 Prov.Park Bay 445326 794437 30-May-03 9.0 8.6 Gibson Lake Latitude Longitude TP1 TP2 Lake Name Township STN Site ID Site Description (DMS) (DMS) Date (µg/l) (µg/l) GIBSON LAKE GEORGIAN BAY 1644 1 S end, deep spot 445725 794432 27-May-03 11.2 10.0 GIBSON LAKE GEORGIAN BAY 1644 2 N end, deep spot 445858 794556 27-May-03 9.8 9.0 GIBSON LAKE GEORGIAN BAY 1644 3 SofNarrows-Deep Spot 445750 794502 15-May-11 11.8 11.6 GIBSON LAKE GEORGIAN BAY 1644 3 SofNarrows-Deep Spot 445750 794502 06-May-12 11.4 12.2 GIBSON LAKE GEORGIAN BAY 1644 3 SofNarrows-Deep Spot 445750 794502 31-May-13 14.8 14.4 GIBSON LAKE GEORGIAN BAY 1644 3 SofNarrows-Deep Spot 445750 794502 14-Jul-14 13.6 13.6 23

Go Home Lake Latitude Longitude TP1 TP2 Lake Name Township STN Site ID Site Description (DMS) (DMS) Date (µg/l) (µg/l) GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 05-May-02 6.7 9.7 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 19-May-03 5.5 9.6 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 09-May-04 5.6 5.7 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 01-May-05 5.6 4.9 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 14-May-06 6.5 6.9 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 06-May-07 5.7 5.4 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 19-May-08 8.7 9.1 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 10-May-09 5.5 7.2 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 16-May-10 6.4 6.0 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 08-May-11 7.2 7.2 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 06-May-12 5.8 6.2 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 05-May-13 5.4 6.0 GO HOME LAKE GIBSON 1667 1 Bay of Many Winds 450026 795042 04-May-14 6.6 6.8 GO HOME LAKE GIBSON 1667 7 Mid Lake, deep spot 450030 795043 27-May-03 6.4 6.2 GO HOME LAKE GIBSON 1667 7 Mid Lake, deep spot 450030 795043 02-Jun-04 6.5 6.0 Galla Lake Latitude Longitude TP1 TP2 Lake Name Township STN Site ID Site Description (DMS) (DMS) Date (µg/l) (µg/l) GALLA LAKE FREEMAN 1607 1 Mid Lake, deep spot 450400 795200 30-May-03 6.6 6.8 GALLA LAKE FREEMAN 1607 2 mid lake deep spont 450377 795191 16-May-09 8.5 6.6 GALLA LAKE FREEMAN 1607 2 mid lake deep spont 450377 795191 06-Jun-10 7.8 8.4 GALLA LAKE FREEMAN 1607 2 mid lake deep spont 450377 795191 22-May-11 7.2 8.0 Stewart Lake Latitude Longitude TP1 TP2 Lake Name Township STN Site ID Site Description (DMS) (DMS) Date (µg/l) (µg/l) STEWART LAKE FREEMAN 5166 1 Mid Lake, deep spot 450830 794538 20-May-03 7.0 7.6 STEWART LAKE FREEMAN 5166 1 Mid Lake, deep spot 450830 794538 07-Jun-04 8.8 9.1 STEWART LAKE FREEMAN 5166 2 mid lake, deep spot 450830 794538 03-May-09 7.3 7.0 STEWART LAKE FREEMAN 5166 2 mid lake, deep spot 450830 794538 02-May-10 7.8 6.6 STEWART LAKE FREEMAN 5166 2 mid lake, deep spot 450830 794538 03-Jun-11 5.6 6.8 STEWART LAKE FREEMAN 5166 2 mid lake, deep spot 450830 794538 20-May-12 7.2 7.2 STEWART LAKE FREEMAN 5166 2 mid lake, deep spot 450830 794538 08-Jun-13 7.6 8.0 STEWART LAKE FREEMAN 5166 2 mid lake, deep spot 450830 794538 01-Jun-14 5.8 5.8 24

Appendix 4 Sample Locations for Inland Lakes (except Galla Lake) 25

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