A quarterly publication containing special offers for significant savings on a variety of molecular biology products CDA winter savings High Quality Costs Less RNAi PCR / qpcr Look inside to find great deals on Thermo Scientific products GeneJET DNA & RNA Cleanup Kits Maxima H Minus cdna Synthesis Kit Luminaris Color qpcr Master Mixes Phusion Hot Start II DNA Polymerase CloneJET PCR Cloning Kit www.uoftmedstore.com DNA Tools one place for all your molecular biology needs thermoscientific.com/onebio
Streamline your cloning workflow with Thermo Scientific Molecular Biology Products Thermo Scientific TurboFect Transfection Reagent egfp 25% off TurboFect Transfection Reagent High transfection efficiency of plasmid DNA into a wide variety of cell types Excellent transfection efficiency in the presence or absence of serum Simple, 3 step protocol ecfp DsRed Triple transfection of HeLa cells using TurboFect Cat. No. Description Size Price $ 25% off R0531 TurboFect Transfection Reagent 1 ml, 500 transfections 341.00 255.75 Unique tools for DNA Methylation research! 50% off EpiJET DNA Methylation Analysis Kits Site-specific methylation analysis in just 1 hour with restriction enzyme isoschizomer pairs EpiJET 5-hmC Analysis Kit Efficient detection of 5-hydroxymethylcytosine (5-hmC) Thermo Scientific EpiJET Kits Reproducible DNA methylation analysis in CpG islands and gene bodies Specially formulated highly stable enzymes and reagents for epigenetics Cat. No. Description Reactions Price $ 50% off K1441 EpiJET DNA Methylation Analysis Kit (MspI/HpaII) 200 158.00 79.00 K1451 EpiJET DNA Methylation Analysis Kit (TaqI/HpyF30I) 200 147.00 73.50 K1481 EpiJET 5-hmC Analysis Kit 25 217.00 108.50
Concentrate on your Research! Thermo Scientific GeneJET DNA and RNA Purification Kits 30% off NEW DNA & RNA Cleanup Genomic DNA Total RNA Viral DNA & RNA GeneJET Gel Extraction and DNA Cleanup Micro Kit Fast and simple 4 minute reaction mixture clean-up and 15 minute agarose gel purification Efficient removal of reaction components including enzymes, proteins, primers dimers, dntps, and salts GeneJET RNA Cleanup and Concentration Micro Kit Fast and simple procedure takes less than 5 minutes Exceptional purity efficiently removes inhibitors such as EDTA and citrate as well as enzymes like DNAse I GeneJET Genomic DNA Purification Kit Universal kit ideal for genomic DNA purification from cells, tissues, whole blood, bacteria and yeast GeneJET Whole Blood Genomic DNA Purification Mini Kit RNA-free genomic DNA from whole blood, bone marrow and cell-free body fluids GeneJET Plant Genomic DNA Purification Mini Kit Streamlined procedure without clogging-prone lysate filtration step. Material specific protocols provided GeneJET RNA Purification Kit Rapid and efficient purification of high quality total RNA from cells, tissues, whole blood, bacteria and yeast GeneJET Whole Blood RNA Purification Mini Kit DNA-free RNA with no DNaseI step Compatible with anticoagulants such as EDTA, citrate and heparin GeneJET Plant RNA Purification Mini Kit Purify high integrity RNA (RIN > 8) without clogging-prone lysate filtration step. Material specific protocols provided GeneJET Viral DNA and RNA Purification Kit Efficient purification of viral DNA & RNA from plasma, serum, blood and cell-free samples GeneJET DNA & RNA Cleanup Kits DNA and RNA recovery rates up to 100% Advanced column design easy to load and handle Small elution volumes as low as 6 μl! M 1 2 3 4 5 6 Total RNA from various sources using the GeneJET RNA Purification Kit. M- Thermo Scientific RiboRuler High Range RNA Ladder (#SM1821) 1- mouse liver, 2- mouse spleen, 3- HeLa cells, 4- Cos-7 cells, 5- Saccharomyces cerevisiae, 6- Escherichia coli DH5α Cat. No. Description Preps Price $ 30% off K0831 50 105.00 73.50 GeneJET DNA Cleanup and Gel Extraction Micro Kit K0832 250 490.00 343.00 K0841 50 175.00 122.50 GeneJET RNA Cleanup and Concentration Micro Kit K0842 250 700.00 490.00 K0721 50 146.00 102.20 GeneJET Genomic DNA Purification Kit K0722 250 650.00 455.00 K0781 50 125.00 87.50 GeneJET Whole Blood Genomic DNA Purification Mini Kit K0782 250 500.00 350.00 K0791 50 156.00 109.20 GeneJET Plant Genomic Genomic DNA Purification Mini Kit K0792 250 625.00 437.50 K0731 50 265.00 185.50 GeneJET RNA Purification Kit K0732 250 1,040.00 728.00 K0761 GeneJET Whole Blood RNA Purification Mini Kit 50 250.00 175.00 K0801 50 250.00 175.00 GeneJET Plant Genomic Genomic DNA Purification Mini Kit K0802 250 1,000.00 700.00 K0821 GeneJET Viral DNA and RNA Purification Kit 50 245.00 171.50
High performance cdna synthesis and qpcr products 30% off NEW Thermo Scientific Maxima H Minus Reverse Transcriptase No RNase H activity - maximum yields of full-length cdna up to 20kb Active up to 65 C Complete cdna synthesis in 15-30 minutes Compatible with a wide range of starting RNA amounts (1 pg 5 µg) Thermo Scientific Maxima H Minus First Strand cdna Synthesis Kit Convenient kit format supplied with a premixed enzyme solution, oligo(dt) 18 and random hexamer primers Thermo Scientific RevertAid H Minus Reverse Transcriptase No RNase H activity - high yields of full-length cdna up to 13 kb Active up to 55 C Thermo Scientific RevertAid H Minus First Strand cdna Synthesis Kit Supplied with RevertAid H Minus, Thermo Scientific RiboLock RNase Inhibitor, oligo(dt) 18 and random hexamer primers plus control RNA in individual tubes NEW Thermo Scientific Luminaris Color qpcr Master Mixes Multicolor system reduces the risk of pipetting errors during reaction set up Premixed with UDG to control for carry-over contamination Sensitive & Specific - Detect low copy number targets, no primer dimers Wide linear range accurate quantification across 9 orders of magnitude Compatible across qpcr platforms - available in probe and hi-green chemistries + = Luminaris Color qpcr Master Mix Blue Master Mix and Yellow Sample Buffer for easy tracking of pipetting steps E = 100.817% R 2 = 0.9975 10 ng 0.1 pg NTC Efficient, specific and sensitive cdna detection Human c-myc gene was amplified with Luminaris Color HiGreen qpcr Master Mix using cdna reverse transcribed from HeLa cells total RNA (10 ng 0.1 pg) with the Maxima First Strand cdna Synthesis Kit for RT-qPCR (#K1641). cdna was subsequently amplified on the Thermo Scientific PikoReal Real- Time PCR instrument. The insert in the graph shows the melting curve analysis results indicating high specificity amplification. NTC = non-template control. Cat. No. Description Size Price $ 30% off K1651 20 rxns 140.00 98.00 Maxima H Minus First Strand cdna Synthesis Kit K1652 100 rxns 588.00 411.60 EP0751 2000 U 70.00 49.00 Maxima H Minus Reverse Transcriptase EP0752 10000 U 232.00 162.40 K1631 20 rxns 135.00 94.50 RevertAid H Minus First Strand cdna Synthesis Kit K1632 100 rxns 540.00 378.00 EP0451 10000 U 200.00 140.00 RevertAid H Minus Reverse Transcriptase EP0452 5x10000 U 820.00 574.00 K0361 Luminaris Color HiGreen High ROX qpcr Master Mix K0362 500 rxns 424.00 296.80 K0371 Luminaris Color HiGreen Low ROX qpcr Master Mix K0372 500 rxns 424.00 296.80 K0381 Luminaris Color HiGreen fluorescein qpcr Master Mix K0382 500 rxns 424.00 296.80 K0331 Luminaris Color Probe High ROX qpcr Master Mix K0332 500 rxns 424.00 296.80 K0341 Luminaris Color Probe Low ROX qpcr Master Mix K0342 500 rxns 424.00 296.80
Versatile PCR enzymes and cloning kits 30% off Phusion DNA Polymerase Use with Maxima H Minus to generate cdna for cloning Taq DNA Polymerase & dntps Thermo Scientific Phusion Hot Start II High Fidelity DNA Polymerase The gold standard for high fidelity hot start PCR - Extreme fidelity - 52X compared to Taq - Robust amplification with less enzyme - Fast cycling short extension times (15-30 s/kb) Zero-time reactivation due to unique Affibody hot start technology Room-temperature set-up, reduction in non-specific amplification and primer degredation Thermo Scientific Phusion Flash High Fidelity Master Mix Convenient supplied as a ready-to-use 2X master mix Extreme speed: extension times of 15 s/kb or less Zero-time reactivation due to unique Affibody hot start technology Accurate: proofreading DNA polymerase with 25X high fidelity compared to Taq Thermo Scientific Taq DNA Polymerase Ideal enzyme for routine PCR Thermostable half life is more than 40 minutes at 95 C Generates PCR products with 3 -da overhangs Thermo Scientific dntp Master Mix Greater than 99% purity confirmed by HPLC Compatible with routine and long PCR applications 1.7 kb 2.2 kb 2.3 kb (GC-rich) M P A B C D M P A B C D M P A B C D M P: Phusion Hot Start II High-Fidelity DNA Polymerase A-D: Proofreading hot start DNA polymerases from major suppliers Phusion Hot Start II DNA Polymerase provides extreme specificity and abundant yields Five proofreading hot start DNA polymerases from major suppliers were used to amplify 1.7-2.3 kb fragments from human genomic DNA. CloneJET PCR Cloning Kit pjet1.2/blunt vector map Thermo Scientific CloneJET PCR Cloning Kit Fast PCR cloning in 5 minutes Versatile ideal for blunt-end or sticky-end cloning No cloning background with the pjet1.2/blunt positive selection vector Cloning Kits Thermo Scientific alicator Ligation Independent Cloning and Expression System Fast directional ligation independent cloning of PCR products in 15 minutes Tight control of protein synthesis for expression of toxic genes in E.coli plate vectors allow for generation of untagged or tagged proteins with the option of enzymatic tag removal Thermo Scientific Rapid DNA Ligation Kit Fast ligation of either sticky- or blunt-end DNA in 5 minutes Convenient reaction mixture can be used directly in bacterial transformation Cat. No. Description Size Price $ 30% off F-548S 100 x 20 µl Phusion Flash High- rxns 112.00 78.40 F-548L Fidelity PCR Master Mix 500 x 20 µl rxns 445.00 311.50 F-549S Phusion Hot Start II High- 100 U 179.00 125.30 F-549L Fidelity DNA Polymerase 500 U 714.00 499.80 EP0402 500 U 122.00 85.40 Taq DNA Polymerase, EP0405 5 x 500 U 510.00 357.00 recombinant EP0406 10 x 500 U 969.00 678.30 R0192 dntp Mix, 10 mm each 1 ml 150.00 105.00 Offers are valid from January 1st until March 31st, 2013. Not to be combined with any other promotions or discounts. Cat. No. Description Size Price $ 30% off K1231 20 rxns 246.00 172.20 CloneJET PCR Cloning Kit K1232 40 rxns 348.00 243.60 K1241 alicator LIC Cloning and Expression Kit 1 (untagged) K1251 alicator LIC Cloning and Expression Kit 2 (N-terminal His-tag/EK) K1261 alicator LIC Cloning and Expression Kit 3 (C-terminal His-tag) K1271 alicator LIC Cloning and Expression Set 1 (All-in-One/EK) 30 rxns 287.00 200.90 K1281 alicator LIC Cloning and Expression Kit 4 (N-terminal His-tag/WQ) K1291 alicator LIC Cloning and Expression Set 2 (All-in-One/WQ) 30 rxns 287.00 200.90 K1422 50 rxns 174.00 121.80 Rapid DNA Ligation Kit K1423 150 rxns 356.00 249.20
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