Tissue Culture Products

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Tissue Culture Products PRODUCT CATALOGUE

We are a fast-growing, dynamic centre of excellence in the field of innovative, high-quality laboratory products. We specialise in Tissue Culture, distinguishing ourselves by creating solutions based on market needs and by providing excellent customer care. Our mission is to offer customers the most innovative products of the highest quality possible. Our products are sold worldwide by specially qualified, professional distributors. Our products are manufactured in 100,000 grade clean-room environment under the control of ISO 9001:2008 and ISO 13485 quality management system. Products with surface treatment are modified with vacuum-gas plasma causing the very hydrophobic polystyrene surface to become negatively charged and hydrophilic, allowing cells to attach and for more even and consistent attachment and growth. Unless specified otherwise, all products included in this catalogue comply with the following: Certified as having been sterilised by gamma radiation Certified as being DNA and RNA free Certified as having an endotoxin level of <0.5 EU/ml All certificates are available upon request Systematic inspection of physical dimensions Automatic quality checks several times along the production lines Final visual inspection of each individual bag before packaging Additional quality and performance tests are also performed regularly by independent laboratories Complete traceability program has been implemented OrFlask Tissue Culture Flasks www.orangesci.com/orflask Orange Scientific s advanced, high-quality Tissue Culture Flasks guarantee an even surface refinement in the growth zone. The special surface treatment no chemicals are used provides an optimal growth surface. Vent stop position allows easy stopping of the cap in the Vent position, and ensures perfect repetition from flask to flask. TISSUE CULTURE PRODUCTS P2

OrFlask Tissue Culture Flasks www.orangesci.com/orflask Orange Scientific flasks are also available with filter caps. A hydrophobic membrane with micro pores allows sterile gas exchange, thus protecting both the culture and the environment from risk of contamination. The micro pores of the membrane prevent the passage of particles larger than 0.2 μm, and therefore they retain bacteria and spores. As the membrane is hydrophobic, the pores will not be clogged even if the liquid in the flask comes in contact with the membrane. Made of USP VI class virgin Polystyrene, high clarity and toughness, high light transferability with minimal light distortion Easy-to-read volume scale Optimum optical clarity for microscope observation Easy and safe stacking Packaged in peel off bags to ease opening and are resealable thus preventing contamination Flasks are certified as having been sterilised by gamma radiation Flasks are certified as being DNA and RNA free Flasks are certified as having an endotoxin level of <0.5 EU/ml All certificates are available upon request CATALOGUE # DESCRIPTION CAP TYPE GROWTH AREA (CM 2 ) VOLUME (ML) UNITS / BAG UNITS / CASE 4410100 25cm 2 Flask Vent 25 50 10 200 4410200 75cm 2 Flask Vent 75 250 5 100 4410300 175cm 2 Flask Vent 182 600 5 40 4420100 25cm 2 Flask Filter 25 50 10 200 4420200 75cm 2 Flask Filter 75 250 5 100 4420300 175cm 2 Flask Filter 182 600 5 40 P3 TISSUE CULTURE PRODUCTS

OrPlate Tissue Culture Test Plates www.orangesci.com/orplate As with all other Orange Scientific products, the Tissue Culture Test Plates are precision engineered and manufactured from high-grade plastics, so that the result is a test plate of the highest quality. A particular advantage of these test plates is that they have especially large, deep spacing between the wells to prevent cross-contamination in case of spills. Made of USP VI class virgin Polystyrene, high clarity and toughness, high light transferability with minimal light distortion Guaranteed even surface refinement in the growth zone Special surface treatment no chemicals are used to provide an optimal growth surface Air extraction design maximises external/ internal gas exchange, while reducing media evaporation without altering CO2 diffusion Alpha-numeric identification method to identify each well Specially designed lid separates each well, thus preventing liquid or condensate crosscontamination Deep spacing between the wells to prevent cross-contamination Cut corner makes the lid a one-way lid Special design allows easy and stable stacking Optimum optical clarity for microscope observation Test Plates are certified as having been sterilised by gamma radiation Test plate are certified as being DNA and RNA free Test plates are certified as having an endotoxin level of <0.5 EU/ml Packaged in peel off bags to ease opening All certificates are available upon request CATALOGUE # DESCRIPTION MAX WELL VOLUME (ML) WORKING VOLUME (ML) CELL GROWTH AREA (CM 2 ) UNITS / BAG UNITS / CASE 4430100 96 Flat Test Plate 0.39 0.075-0.20 0.33 1 100 4430200 96 U Test Plate 0.32 0.32 0.32 1 100 4430250 48 Flat Test Plate 1.55 0.19-0.29 0.84 1 100 4430300 24 Flat Test Plate 3.50 0.38-0.57 1.93 1 100 4430400 12 Flat Test Plate 6.80 0.76-1.14 3.85 1 100 4430500 6 Flat Test Plate 17.0 1.90-2.90 9.60 1 100 TISSUE CULTURE PRODUCTS P4

OrDish Tissue Culture Dishes www.orangesci.com/ordish Orange Scientific Tissue Culture Dishes provide several innovative improvements over the conventional dishes. A rim around the dish ensures safe, easy pick up and handling on the 60 and 100 diameter dishes. Feeling the rim indicates that the entire dish is picked up and not just the cover. Made of USP VI class virgin Polystyrene, high clarity and toughness, high light transferability with minimal light distortion Guaranteed even surface refinement in the growth zone Special surface treatment no chemicals are used to provide an optimal growth surface Optimum optical clarity for microscope observation Packaged in peel-off bags to ease opening and are resealable thus preventing contamination Dishes are certified as having been sterilised by gamma radiation Dishes are certified as being DNA and RNA free Dishes are certified as having an endotoxin level of <0.5 EU/ml All certificates are available upon request CATALOGUE # DESCRIPTION DIAMETER (MM) DIMENSIONS (MM) CELL GROWTH AREA (CM 2 ) UNITS / BAG UNITS / CASE 4450100 35mm Dish 32.8 12.5 X 37.8 8.5 10 960 4450200 60mm Dish 52.0 17.8 X 58.8 21.2 10 600 4450300 100mm Dish 87.8 22.0 X 95.6 60.8 10 300 4450400 150mm Dish 135.5 21.9 X 143.0 143.0 1 120 P5 TISSUE CULTURE PRODUCTS

OrTube Centrifuge Tubes www.orangesci.com/ortube Orange Scientific high-quality Centrifuge Tubes feature exceptional transparency. Special attention has been devoted to the cap system in order to ensure complete, safe tightness even when subjected to vibrations. Centrifugal Force: Conical Tubes up to 12000 x G Skirt Tubes up to 6000 x G Admissible Temperature: -80 C to +121 C Made of either Highly transparent, excellent chemical resistance clarity and gloss, anti static Polypropylene, high clarity and toughness, high light transferability with minimal light distortion Precise, printed graduation along the tube and engraved graduation on the cone to allow easy reading of small volumes Clear white marking area Sterile tubes are packaged in peel-off bags to ease opening and are resealable thus preventing contamination Tubes are made of high-quality polypropylene Sterilised tubes are certified as having been sterilised by gamma radiation Sterilised tubes are certified as being DNA and RNA free Sterilised tubes are certified as having an endotoxin level of <0.5 EU/ml CATALOGUE # DESCRIPTION STERILISED VOLUME (ML) UNITS / BAG UNITS / CASE 4440100 50ml Tube conical Yes 50 25 500 4440120 50ml Tube conical Yes 50 25 / Rack 500 4440150 50ml Tube conical No 50 50 500 4440200 50ml Tube skirt Yes 50 25 500 4440300 15ml Tube conical Yes 15 25 500 4440320 15ml Tube conical Yes 15 25 / Rack 500 4440350 15ml Tube conical No 15 50 500 TISSUE CULTURE PRODUCTS P6

OrPipette Serological Pipettes www.orangesci.com/orpipette Orange Scientific Serological Pipettes are specially designed for Tissue Culture. The Serological Pipettes are manufactured from a single piece of high-grade polystyrene. No part of the pipette is welded to the body. Consequently, the pipettes have no welding points, so no liquid can be trapped at such points. Certified as having been sterilised by gamma radiation Certified Non-Pyrogenic Certified Non-Cytotoxic Certified Non-Hemolytic Negative graduation provides additional volume A plug is inserted into the top end to prevent liquid from penetrating pipettors Colour-coding system makes identifying the pipette volume quick and easy Packaged in peel-off bags to ease opening CATALOGUE # VOLUME (ML) COLOR CODE GRADUATION (ML) NEGATIVE GRADUATION (ML) UNITS / CASE PN1E1 1 Yellow 1/100 0.3 500 PN2E1 2 Green 1/50 0.4 500 PN5E1 5 Blue 1/10 3 250 PN10E1 10 Orange 1/10 3 200 PN25E1 25 Red 1/5 2.6 100 7306 50 Purple 1/2 10 100 OrScrapers Cell Scrapers www.orangesci.com/orscrapers Orange Scientific Cell Scrapers are specially developed to make the work of scraping off and collecting cells easier and more effective. The scrapers include a special blade that can be positioned either in scraping position (blade aligned with the handle) or in the collecting position (blade perpendicular to the handle). The blade presents the right softness in order to collect cells without damaging them. Orange Scientific Cell Scrapers are sterilised by gamma radiation and packaged individually. CATALOGUE # LENGTH (CM) BLADE WIDTH (CM) UNITS / CASE 4460100 25 2.0 100 4460200 39 3.0 100 P7 TISSUE CULTURE PRODUCTS

Graignette Business Park Avenue du Commerce 32 B-1420 Braine-L Alleud Belgium tel.: +32 3 387 56 97 fax: +32 2 387 00 93 email: info@orangesci.com web site: www.orangesci.com (c/o ProdWal SPRL - VAT: BE 0848.358.733) OTHER AVAILABLE PRODUCTS Liquid Handling Instruments www.orangesci.com/lhinst Liquid Handling Tips www.orangesci.com/lhtips Test Tubes, PCR Tubes www.orangesci.com/testtubes Dialysis membranes www.orangesci.com/dialysismemb Laboratory Filtration www.orangesci.com/filt More products are available on our website: www.orangesci.com Local Distributor OS TC 12.2012