MIC and MBC Evaluation of Pure Products Antimicrobial Product against Selected Bacteria:
|
|
- Jason Gallagher
- 6 years ago
- Views:
Transcription
1 01/28/ :40 FAX MOTHER EARTH MINERALS 14)001 1/28/16 To: Jim Dooley From; Mother Earth Minerals (Five Pages) MIC and MBC Evaluation of Pure Products Antimicrobial Product against Selected Bacteria: Staphylococcus aureus E.coli
2 - 01/28/ :4 0 FAX MOTHER EARTH 003 Rese a rc h Cor pora t ion 3437 SW 24 Avenue fox: Gir.mdllc, Ft. ;.2607 E-rmiil: rnfo -Oabr:r.rum l'el: ,-0436 Web: A FOOD TESTING LABORATORY SlNCE 1967 PROJECT REPORT RESEARCH MICROBIOLOGY DEPARTMENT DAIE: August 29, 2006 PREPARED FOR: CLIENT CONTACT: OBJECTIVE: EXPERIMENTAL APPROACH: A!ESTPRODUCT AND.MATERIALS MIC and MBC Evaluation of Pure Products Antimicrobial Product against Selected Bacteria. To dcterm.i.ne the MIC (minimal inhibitory concentration) and MBC (miuimal bactericidal concentration (MBC) for Pure Products antinlicrobi.nl product against E. coli and Staphylococcus aureus using standard NCCLS/CLSl roethods. The client provided sufficient Pure Products antimicrobial product to use in the study. B. TEST MICROOROANISMS Generic Escherichia coli (ATCC #11229) and methicillin resistant Staphylococcus aureus (ATCC #700698) were used.in the MIC and MBC evaluations. Generic E. coli is a common Gram-negative bacterial indicator of human/animal feces in food and water. S. aurew is 11 common Gram-positive bacterial pathogen associated with clinical (e.g_, skin and nosocomial infections). Antibiotic resistant strains of S. aureus are of particular clinical concern. C. MIC AND MBC PROCEDURES MIC (minimal inlubitory concentration) assays for the antimicrobial were conducted separately against each selected microorganism (see section B) using the broth macro-dilution procedure for antibiotic susceptibility testing per NCCLS/CLSl methods (''National Committee for Clinical Laboratory Sta.ndatds"; now.referred to as "Clinical and Laboratory Standards Institute"). The inoculum for each microorganism was prepared and the MlC assay conducted. according to respective culture methods de5cribcd by NCCLS (M7-A5, 2000). The E. coli and S. aureus cultures were purified and cultured ming pre-poured Tryptic Soy agar (TSA) plates (incubated.for h. at 35 C). Bacterial suspensions were each prepared from the respective ISA plates and diluted per NCCLS method (to a 0.5 McFarland standard
3 01/28/ :40 FAX MOTHER EARTH AquaVivos (dba Pure Products) Its Effectiveness Against E.Coli Recent tests were conducted to determine the effectiveness of AquaVivos (dba Pure Products) against Escherichia coli (E. coli) bacteria. The tests were conducted over a 24 hour period by ABC Research Corporation. a food testing laboratory, in Gainesville, FL. Table 2. Minimal Bactericidal Concentration (l\'lbc) Results for E. coli. 1\ntimkrobinl lbctl.rii Counts CFU.iml l.oqrn C FU/ml Initial fooculum c;'ii r I,, J :6 Sun'h or Coun 1 <10 <1.HO l.01!1, RNI ucti,,n >4.77!,. Rt d uct'iun > J :12 Sun h or Conni <10 <l.00 Loit1111 Rtduction :>4.17 It> R\'duction >99.1)98 1:24 Sunfror Cl)unt..: 10 <1.00 lo-.. Rt dt1cf i11n >4.77 % Rl'd uction ;: bl8 S!:!n'lt or Coml1 <10 <1.00 l.0 1, Rl cl ucfom >4.77 % Rtdudion > :% Sllnin,r Co1m1 t S I,f'lg 11 ]l1. duco,,n 2.69 "!., Rc. duction 99.8 Noti;-s: l ) (\, 11':\l:- xpn.:s o.:j ::i ('f l.i \\, km)" fr,rm in&! un.it/ml). 2) l.\l Rl.!d llt.:l inn (lo!:,! 0.:\1U1H f initinl in\'ul um) - iii' count,.,f ulk t survin.1r:-.)..1} Th MBC!i...:..,ll kalll n -h)g \'J' 99. l)'ji, rcducli\,.ll ) w.is al 1 1:4 dilutk,n "'t' t.h an1i1nk1'<,h i,.i. The dilution rate of 1:48 (1 part AquaVivos to 48 parts water) provided the most efficient results, reducing the E. colibacteria %.
4 01/28/ :41 FAX fother EARTH MINERALS 14]004 Page 2 of 4 in sterile 0.85% saline) to obtain respective cell suspensions to inoc'ltlate the rnacrodihrtion series. The final inoculum auspensions were enumerated by making appropriate serial dilutions in Butterfield's phosphate buffer (BPB) and spread plating 0.1 ml of each dilution onto pre-poured TSA plates. The coi:respomling MIC assays were conducted using Mueller Hinton broth (MHB) to prepare the macro-dilution series of the antimicrobial product (i.e., from 1 :6 through 1;24576) in M.HB. The (MIC) dilution tubes were incubated for 24 h at 35QF. The MIC is the lowest concentration of antimicrobiaj agent that completely inhibits growth of the bacteria. Minimal bactericidal concentration (MBC) assays were determined concw:rently against each microorganism per standard methods (i.e., Manual of Clinical Microbiology, 2003). After incubation of the MHB tubes and detcnnination of the MIC, the surviving bacteria in each tube with concentrations at and above the MIC was enumerated in duplicate using spread plating technique and pre-poured TSA plates. Using the initial.inoculwn counts and the viable counts in respective MHB tubes, t..1..e lethality of each res,pective tube was determined. The MBC is the lowest concentration of antimicrobial which yields at least 99.9% reduction (i.e., 3 log-units) during the MIC incubation (i.e., 24 h at 35 C). RESULTS: The minimal inhibitory concentration (MIC) results for. the Pure Product antimic.robia1 against E. coli and Staphylococcus aureus are presented in Table 1. The MIC value for the antimicrobial against E. coli was at the 1:48 dilution (i.e.) 2.08% concentration of active agent) and that for the S. aureus at the l :24 dilution (i.e., 4.17% concentration of active ageot). The minimal bactcrici.dru concentration (MBC).results for the Pure Product antimic.rnbial against E. coli and Staphylococcus aureus are presented in Tables 2 and 3, respectively. Note th.at these bactericidal (reduction) results apply to a 24 h period in a growth medi'un1per the; standard NCCLS/CLSI methods. There was a greater than 4.77 logwunit (>99.998%) reduction of E. coli at the antimicrobial 's l :48 dilution and a 3.08 log10-unit reduction (99.8%) at the 1:96 dilution. For S. atireus, there was a 2.11 log 1o-unit (99.2%) reduction at the l ;6 dilution, a 2.13 Jog 1o-unit (99.3%) reduction at the 1:12 dilution, and a l.13 1og 1o-unit reduction (92.6%) at the 1:24 dilution. In summary, these results indicated that the PUi\'i Pr:oduct antimicrobial was more effective in the inhibition and reduction of E. coli (a Gram-negative bacterium) than of S. aureus (a Grampositive bacterium). James E. (Ken) Kennedy, Ph.D. Vice President., Research Microbiology ABC Research Corp.
5 01/28/ :42 FAX MOTHER EARTH Page 3 of 4 Table 1. Minimal Inhibitory Concentration (MIC) Results for E. coli and S. aureus Anthnlcrobial E. coli (growth) $. IJW'eU.'J (l!rowth) 1: :12-1:24 - -lmicl 1:48 - lmic) + l: : : : ; l: : , : Note: 1) The MIC is the lowest concentrntion of antimicrobial agent that completely inhibits growth of the bucteria. 2) Toe MlC was at the 1:48 dilution of the a,o.timicrobiri for E. coli and the I :24 dilution of the a.ntimkrobial for!;'. aureus.
6 01/28/ :42 FAX MOTHER EARTH Page 4 of 4 Table 2. Minimal Bactericidal Concentration (MBC) Results for E. coli. Antimicrobial - - Bacterial Counts CFU/ml Log 10 CFU/ml Initial Inocuh1m 590, :6 Sul"'iVor Cowit <10 <l.00 Log10 R.edudlon >4.77 % Reduction > :12 Survivor Co,Jnl <10 <l.00 Lo210 Reduction >4.77 % Red111:tton > :24 Survivor Coun1 <10 <l.00 Lo210 Reduction >4,77 % Reduction :> :48 Sunolvor Cou111 <10 <1.00 Log10 Reduction >4.77 8/u Reduction :> ;96 SW'Vlvor Couni 1., Loirn Reduction 2.69 % Reductio11 99,8 Notes: 1) Couots expressed as CFU (colony forming units/ml). 2) Log Reduction = (log count of initial {noculum) - (log count of subject survivors). 3) Th1,; MBC (i.e., at least a 3-log or 99.9% reduction) was at a 1:48 dilutio.n of th<: antimicrobial. Tab)e 3. Minimal Bactericidal Concentr11tion (MBC) 'Results for $. aureus. Antimict'obtal Baci:eria.l Counts CFU/ml Login CFU/anl Initial Inoeuhm1 270, ;6 Survivor Counc Log 18 Reduction 2.11 o/u Rllduclf o11 99.Z t:u Survivor Couni 2, LOf!tu Reduction 2.13 % Reduction :14 Survi'\lor Count 20, Login Reduction 1.13 % Reduction 92.6 Notes: 1) Counts expressed as CFU (colony fonning units/ml). 2) Log Reduction = (log count of initial jnoculum) - (log count of 6Ubject survivo:ri;). 3) The MBC (i.e., at least a 3 log or 99.!)%.reductioo) was not achieved at any dilution of the ant.in:ucrobial.
Determination of MIC & MBC
1 Determination of MIC & MBC Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight
More informationDetermination of MIC & MBC
1 Determination of MIC & MBC Minimum inhibitory concentrations (MICs) are defined as the lowest concentration of an antimicrobial that will inhibit the visible growth of a microorganism after overnight
More informationTest Method for the Continuous Reduction of Bacterial Contamination on Copper Alloy Surfaces
Test Method for the Continuous Reduction of Bacterial Contamination on Copper Alloy Surfaces Test Organisms: Staphylococcus aureus (ATCC 6538) Enterobacter aerogenes (ATCC 13048) Pseudomonas aeruginosa
More informationTest Method for Efficacy of Copper Alloy Surfaces as a Sanitizer
Test Method for Efficacy of Copper Alloy Surfaces as a Sanitizer Test Organisms: Staphylococcus aureus (ATCC 6538) Enterobacter aerogenes (ATCC 13048) Pseudomonas aeruginosa (ATCC 15442) Methicillin Resistant
More informationStudy Title Antibacterial Activity and Efficacy of KHG FiteBac Technology Test Substance Using a Suspension Time-Kill Procedure
Study Title Antibacterial Activity and Efficacy of KHG FiteBac Technology Test Substance Using a Suspension Time-Kill Procedure Test Method ASTM International Method E2315 Assessment of Antimicrobial Activity
More informationABC. Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline. Volume 19 Number 18
M26-A ISBN 1-56238-384-1 September 1999 ISSN 0273-3099 Methods for Determining Bactericidal Activity of Antimicrobial Agents; Approved Guideline Volume 19 Number 18 Arthur L. Barry, Ph.D. William A. Craig,
More informationPersistence of Activity of a Hand Sanitizer
Persistence of Activity of a Hand Sanitizer Eurofins Microbiology Laboratories, Inc. 2430 New Holland Pike Lancaster, PA 17601 717-556-7373 www.eurofinsus.com TABLE OF CONTENTS 1.0 OBJECTIVE... 3 2.0 PROTOCOL
More information3.0. Materials and methods
63 3.0. Materials and methods 3.1. Plant materials and preparation of extracts Salacia oblonga plants were collected from Western Ghats, Karnataka, India. S. oblonga (RRCBI 7881) authentication was done
More informationResult:COMPLETE Report Date: December 28 th, 2015
Send to: Clean Water Environmental, LLC 1939 Talamore Court Southeast, Grand Rapids, MI 49546 Dr. Dale Williams Result:COMPLETE Report Date: December 28 th, 2015 Customer Name: Clean Water Environmental,
More informationFINAL REPORT. Bacterial Survival on PURE-LIGHT SANI-LIGHT coated Glass Slides. Order Number: PREPARED FOR. PURE-LIGHT Technologies LLC
FINAL REPORT Bacterial Survival on LIGHT coated Glass Slides Order Number:371809760 PREPARED FOR PURE-LIGHT Technologies LLC Michael A. Spears 7/5/2018 EMSL Analytical, Inc. 200 Rt. 130 N, Cinnaminson,
More informationFINAL REPORT. Bacterial Survival on Glass and Laminated Wood Surfaces Exposed to PURE-LIGHT Coated LED Light Bulbs. Order Number:
FINAL REPORT Bacterial Survival on Glass and Laminated Wood Surfaces Exposed to PURE-LIGHT Coated LED Light Bulbs Order Number: 371725740 PREPARED FOR PURE-LIGHT Technologies LLC 3938 North 240 East Rigby,
More informationKILL TIME STUDIES Antimicrobial Activity of Advanced Cellular Silver (ACS) 200 Using Methicillin resistant Staphylococcus aureus (MRSA)
KILL TIME STUDIES Antimicrobial Activity of Advanced Cellular Silver (ACS) 200 Using Methicillin resistant Staphylococcus aureus (MRSA) Test Solution: ACS 200 Submitted August 19, 2008 September 4, 2008
More informationSubmitted May 16, May 23, 2014 BY:
KILL-TIME PROTOCOL Antimicrobial Activity of Five Silver-based Solutions Using Methicillin-resistant Staphylococcus aureus (MRSA) Test solutions: A, B, C, D, E Submitted May 16, 2014 May 23, 2014 BY: Richard
More informationBiocidal Surface Test - Clinell Wipes ~ Project Report Prepared for GAMA Healthcare Ltd ~ Huddersfield Microbiology Services Oct 06
Biocidal Surface Test - Clinell Wipes ~ Project Report Prepared for GAMA Healthcare Ltd ~ Huddersfield Microbiology Services Oct 06 Summary The ability of Clinell wipes to disinfect contaminated steel
More informationAntibiotic Susceptibility Testing (ABST/AST)
Antibiotic Susceptibility Testing (ABST/AST) Goal Offer guidance to physicians in selecting effective antibacterial therapy for a pathogen in a specific body site. Performed on bacteria isolated from clinical
More informationStudy Title Antimicrobial Activity and Efficacy of Seal Shield's Electroclave. Test Method Custom Device Study. Study Identification Number NG7233
Study Title Antimicrobial Activity and Efficacy of Seal Shield's Electroclave Test Method Custom Device Study Study Identification Number NG7233 Study Sponsor Christian Davis Seal Shield 3105 Riverside
More informationCOUNT METHOD 5.0 OBJECTIVES 5.1 INTRODUCTION 5.2 PRINCIPLE. Structure
Food Microbiology EXPERIMENT 5 STANDARD PLATE COUNT METHOD Structure 5.0 Objectives 5.1 Introduction 5.2 Principle 5.3 Materials Required 5.4 Procedure 5.4.1 E-coli Culture 5.4.2 Food Samples 5.5 Observations
More informationAntimicrobial activity (in vitro) of polysaccharide gel from durian fruit-hulls
ORIGINAL ARTICLE Antimicrobial activity (in vitro) of polysaccharide gel from durian fruit-hulls Vimolmas Lipipun 1, Nantawan Nantawanit 2 and Sunanta Pongsamart 3 Abstract Lipipun, V., Nantawanit, N.
More informationM. Ben-David 1, O. Hammer 1, A.Shinderman 1, Y. Gluckman- Yavo 1, M. Fridman 1, D. Gohman 1, G. Ingber 1 and E. Zahavy 2
437 Fast Antibiotic Susceptibility Testing Utilizing a Unique Spectral Intensity Ratio Analysis via Single Fluorescence Membrane Dye Staining and Flow Cytometry M. Ben-David 1, O. Hammer 1, A.Shinderman
More informationSeptember 8, MunnWorks, LLC 150 N. Macquesten Parkway Mount Vernon, NY 10550
September, 2017 To: Max Munn MunnWorks, LLC 150 N. Macquesten Parkway Mount Vernon, NY 10550 From: Matthew Hardwick, PhD ResInnova Laboratories 07 Colesville Rd, 3 rd Floor Silver Spring, MD 20910 Dear
More informationSubmitted May 16, May 23, 2014 BY:
KILL-TIME PROTOCOL Antimicrobial Activity of Five Silver-based Solutions Using Methicillin-resistant Staphylococcus aureus (MRSA) Test solutions: ph Structured Silver, Argentyn 23, ACS 200 Extra Strength,
More informationPRESERVATIVE EFFICACY TEST FOR COSMETIC PRODUCT
1 SCOPE AND FIELD OF APPLICATION To determine the efficacy of the antimicrobial activity of preservatives used in cosmetic products. The method covers the determination of the suitability of preservation
More informationASSESSMENT OF THE MICROBICIDAL ACTIVITY OF AN ACCELERATED HYDROGEN PEROXIDE- BASED FORMULATION (AHP-5) AGAINST VRE AND MRSA
Final Report submitted to Virox Technologies Inc. Oakville, Ontario ASSESSMENT OF THE MICROBICIDAL ACTIVITY OF AN ACCELERATED HYDROGEN PEROXIDE- BASED FORMULATION (AHP-5) AGAINST VRE AND MRSA Syed A. Sattar,
More informationChapter 9 Antimicrobial Susceptibility Testing (Agar Disk Diffusion Method)
Chapter 9 Antimicrobial Susceptibility Testing (Agar Disk Diffusion Method) The disk diffusion method presented in this chapter has been carefully standardized by the National Committee for Clinical Laboratory
More informationLarge Volume Serial Dilutions:
Serial Dilutions All three bacterial plate count methods described in lab require you to serially dilute your samples until you have 30-300 colony forming units (CFU) on the plate. Plates with more than
More informationFINAL REPORT Efficacy of a Cold Plasma System
FINAL REPORT Efficacy of a Cold Plasma System ORDER Number PREPARED FOR: 714 Mall Blvd., Suite 3 Savannah, GA 31406 EMSL Analytical, Inc. Web: http://www.emsl.com - 1 - Certificate of Analysis Client:
More informationJKMU. Antimicrobial Activity of Combined Extracts of Trachyspermum, Thymus and Pistachio against Some Pathogenic Bacteria ARTICLE INFO.
JKMU Journal of Kerman University of Medical Sciences, 2018; 25 (2): 153-163 Antimicrobial Activity of Combined Extracts of Trachyspermum, Thymus and Pistachio against Some Pathogenic Bacteria Fatemeh
More informationExercise 13 DETERMINATION OF MICROBIAL NUMBERS
Exercise 13 DETERMINATION OF MICROBIAL NUMBERS Introduction When biologists discuss the growth of microorganisms (microbial growth), they are actually referring to population size rather than to the size
More informationFactors Influencing Detection of Tolerance in Staphylococcus aureus
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Sept. 1982, p. 364-368 Vol. 22, No. 3 0066-4804/82/090364-0$02.00/0 Copyright 1982, American Society for Microbiology Factors Influencing Detection of Tolerance in
More informationIN THIS SECTION MICROBIOLOGY TESTING EXPERT SOLUTIONS FOR PRODUCT DEVELOPMENT. Bacterial Endotoxin (LAL) Testing
EXPERT SOLUTIONS FOR PRODUCT DEVELOPMENT IN THIS SECTION MICROBIOLOGY TESTING Microbial assays involve a variety of tests, from the determination of the numbers and types of organisms naturally present
More informationIntroduction. Results
E valuation of Inhibitory Data of Essential Oil Constituents Obtained w i t h Different Microbiological Testing Methods A. Pauli and K.-H. Kubeczka Department of Pharmaceutical Biology, University of Hamburg,
More informationMicrobial Survival. Created on 2/25/ :05 AM
Microbial Survival Introduction The main objectives of this practical are: Verify, or otherwise, the Department of Health recommendation that food be cooked to 70 o C for 2 minutes or an equivalent time-temperature
More informationEM021. Co-Trimoxazole Ezy MIC TM Strip (COT)( mcg/ml) (Trimethoprim/ Sulphamethoxazole) Antimicrobial Susceptibility Testing
Co-Trimoxazole Ezy MIC TM Strip (COT)(0.002-32 mcg/ml) (Trimethoprim/ Sulphamethoxazole) Antimicrobial Susceptibility Testing For In Vitro Diagnostic use EM021 Not for Medicinal Use It is a unique MIC
More informationCONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS
CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS Specific control measures can be used to kill or inhibit the growth of microorganisms. A procedure which leads to the death of cells is broadly
More informationPublished Standards ISO General instructions for microbiological examination ISO Enumeration and detection of aerobic mesophilic bacteri
ISO Standards Cosmetics Microbiology TC 217 WG1 Joyce Beauchamp L OREAL USA Products, Inc. 2010 PCPC Microbiology Workshop ISO TC 217 WG1 Microbiological standards and limits Secretariat IRISI Secretary
More informationKey words: Paracetamol, antibacterial activity, chemical preservative, zone of inhibition.
In Vitro Assessment of Antibacterial Activity of lamatecara Preservatives Abstract: The objective of the current research was to evaluate the efficacy of different preservatives of paracetamol syrup against
More informationAssessment of Antibacterial Efficacy of Goldshield against MRSA and VRE
Assessment of Antibacterial Efficacy of against MRSA and VRE Sheri Maxwell, B.S. Charles P. Gerba, Ph.D. Department of Soil, Water and Environmental Science University of Arizona Tucson, Arizona. 85721
More informationINTRODUCTION Contaminated serial dilution countable plates
INTRODUCTION In recent days, the foods that we consume are usually pre-processed in a facility removed from our home, cities, countries, and even continents. It is now more than ever important to be aware
More informationSanitization of ÄKTA pilot 600 using sodium hydroxide
GE Healthcare Sanitization of ÄKTA pilot 600 using sodium hydroxide The ÄKTA pilot 600 chromatography system is designed to facilitate sanitization, with the high standards imposed by regulatory authorities
More informationEzy MIC Strip FEATURES AND ADVANTAGES
Imipenem with & without EDTA Ezy MIC Strips (IPM+EDTA/IPM) (Imipenem + EDTA: 1-64 mcg/ml) (Imipenem : 4-256 mcg/ml) Antimicrobial Susceptibility Testing For In Vitro Diagnostic use EM078 Not for Medicinal
More informationPURE CULTURE TECHNIQUES
PURE CULTURE TECHNIQUES Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (or other microorganisms). A single gram of feces, for example,
More informationBiofilm Protocol Optimization For Pseudomonas aeruginosa. Introduction. Materials and Methods. Culture Media, Incubation Time, and Biofilm Measurement
Biofilm Protocol Optimization For Pseudomonas aeruginosa Culture Media, Incubation Time, and Biofilm Measurement Introduction In addition to the conventional arsenal of antibiotic resistance mechanisms
More informationLab Three :. Sensitivity test:
Lab Three :. Sensitivity test: Or Diffusion Test: Antibiotic sensitivity test: is a laboratory method for determining the susceptibility of organisms to therapy with antibiotics, Antibiotic susceptibility
More informationSlate Steel (Mild Steel) Ceramic
Demonstrating the efficacy of the Dry Ice Blast Cleaning Process against bacterium Escherichia coli, Salmonella Enteritidis, and Listeria monocytogenes on a range of different substrates in association
More informationINTRODUCTION Sanitization sterilization Antibiotics Bactericidal Bacteriostatic Antiseptics disinfectants
INTRODUCTION Infectious agents on environmental surfaces, given the correct circumstances, may potentially find their way into an unsuspecting victim. Thus, it is important to keep the surfaces we regularly
More informationAdaptation of a Bacterial Growth Detection Assay on the VICTOR Nivo Multimode Plate Reader for Measurement of Antibiotic Effects
APPLICATION NOTE Multimode Detection Authors: Maria Kuzikov Dr. Bernhard Ellinger Fraunhofer IME ScreeningPort Hamburg, Germany Adaptation of a Bacterial Growth Detection Assay on the VICTOR Nivo Multimode
More informationENVR 421 Laboratory #1: Basic Bacteriology Techniques
ENVR 421 Laboratory #1: Basic Bacteriology Techniques Introduction The purpose of this laboratory exercise is to familiarize you with two fundamental bacteriology techniques: the streak plate and the spread
More informationStability of Antibiotics and Chemotherapeutics in
APPUED MICROBIOLOGY, Sept. 1970, p. 447-451 Copyright 1970 American Society for Microbiology Vol. 20, No. 3 Printed in U.S.A. Stability of Antibiotics and Chemotherapeutics in Agar Plates KENNETH J. RYAN,
More informationCOMPARING THE ANTIMICROBIAL ACTIVITY OF THE SEMCO 3A TOTAL ENERGY WHEEL WITH OTHER SELECTED ANTIMICROBIAL SURFACE TREATMENTS RESEARCH FINDINGS
AIR QUALITY ANTIMICROBIAL EFFICIENT COMPARING THE ANTIMICROBIAL ACTIVITY OF THE SEMCO 3A TOTAL ENERGY WHEEL WITH OTHER SELECTED ANTIMICROBIAL SURFACE TREATMENTS By Sidney A. Crow, Jr. 1 True 3Å Wheel The
More informationControl Issued by: LABORATORY MANAGER Original Date: April 11, 2001 Approved by: Laboratory Director Revision Date: February 27, 2004
Section: Policy # MI\QC\05\v02 Page 1 of 5 Subject Title: Kirby Bauer Quality Control Issued by: LABORATORY MANAGER Original Date: April 11, 2001 Approved by: Laboratory Director Revision Date: February
More informationStaphylococcus aureus
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, Nov. 1980, p. 784-788 0066-4804/80/11-0784/05$02.00/0 Vol. 18, No. 5 Effect of Storage and Changes in Bacterial Growth Phase and Antibiotic Concentrations on Antimicrobial
More informationIsolation and Characterization of Two Antibiotic-Producing Bacteria
Isolation and Characterization of Two Antibiotic-Producing Bacteria Madeline Gibson Abstract The discovery of antibiotics with novel mechanisms has plateaued in the last twenty years. As antibiotics are
More informationAntibiotic Susceptibility Testing and Data Interpretation
Antibiotic Susceptibility Testing and Data Interpretation Dr Shabbir Simjee Microbiologist Co-Chair CLSI VAST Basingstoke England Bangkok, 7-8 October 2014 For clarity, these are solely my personal views/opinions
More informationAlpha HydroMAID Cleaning Effectiveness of the Alpha HydroMAID Cleaning System versus Conventional Mopping
The purpose of these studies was to evaluate the effectiveness of the Alpha HydroMAID (Mobile Automated Integrated Diluter) for removing a bacterial/fungal mixture typical of a kennel floor bioload and
More informationá61ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: MICROBIAL ENUMERATION TESTS
USP 40 Microbiological Tests / á61ñ Microbiological Examination 1 á61ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: MICROBIAL ENUMERATION TESTS INTRODUCTION The tests described hereafter will allow
More informationKILL-TIME STUDIES Antimicrobial Activity of Experimental Solutions Using Legionella pneumophila Test Solution: ACS 200 Submitted June 6, 2012
KILL-TIME STUDIES Antimicrobial Activity of Experimental Solutions Using Legionella pneumophila Test Solution: ACS 200 Submitted June 6, 2012 October 11, 2012 PREPARED FOR: Results RNA 1272 South 1380
More informationMeropenem: in-vitro activity and kinetics of activity against organisms of the Bacteroides fragilis group
Journal of Antimicrobial Chemotherapy (99) 7, 599-606 Meropenem: in-vitro activity and kinetics of activity against organisms of the Bacteroides fragilis group J. A. Garcia-Rodriguez, J. E. Garcia Sanchez,
More informationImmobilization and Death of Bacteria by Flora Seal Microbial Sealant
International Journal of Pharmaceutical Science Invention ISSN (Online): 2319 6718, ISSN (Print): 2319 670X Volume 6 Issue 6 June 2017 PP. 45-49 Immobilization and Death of Bacteria by Flora Seal Microbial
More informationRate of Penicillin Killing of Staphylococcus aureus and
JOURNAL OF CLINICAL MICROBIOLOGY, Feb. 1982, p. 27-274 95-1137/82/227-5$2./ Vol. 15, No. 2 Rate of Penicillin Killing of Staphylococcus aureus and Autobac 1 Susceptibility Test Results JO-ANN HARRIS' AND
More informationDocument No. FTTS-FA-001. Specified Requirements of Antibacterial Textiles for General Use
1. Purpose and Scope This criterion is applicable to the evaluation and testing of antibacterial activity of textile for general use. The quantitative evaluation of antibacterial activity is judged by
More informationTRYPTIC SOY AGAR (TSA) WITH LECITHIN AND TWEEN 80
TRYPTIC SOY AGAR (TSA) WITH LECITHIN AND TWEEN 80 Cat. no. P45 TSA with Lecithin and Tween 80, 15x60mm Contact Plate, 15ml Cat. no. Q13 TSA with Lecithin and Tween 80, 20x125mm Tube, 18ml Deep Cat. no.
More informationMICROBIOLOGICAL EXAMINATION OF NON-STERILE PRODUCTS: TEST FOR SPECIFIED MICRO-ORGANISMS Test for specified micro-organisms
5-2-3. Most-probable-number method Prepare and dilute the sample using a method that has been shown to be suitable as described in section 4. Incubate all tubes at 30-35 C for 3-5 days. Subculture if necessary,
More informationDecontamination Effectiveness of Esco Celsafe CO2 Incubator Sterilization Feature using High Heat Temperature By Bekti Tri Sumaryati
Decontamination Effectiveness of Esco Celsafe CO2 Incubator Sterilization Feature using High Heat Temperature By Bekti Tri Sumaryati 1. Summary Esco Celsafe CO 2 Incubator sterilization feature, which
More informationStandard Operating Procedure Title: Stock Suspensions of Micro-Organisms
2. Correct Aseptic technique must be used when performing all Microbiological procedures. 3. All work must be carried out in the Biohazard Cabinet. Table of Contents 2. Positive Identification of Reference
More informationANTIMICROBIAL ACTIVITY OF OZOILE (Ozono Topico stabilizzato in Acido Oleico con Vitamina E acetato)
ANTIMICROBIAL ACTIVITY OF OZOILE (Ozono Topico stabilizzato in Acido Oleico con Vitamina E acetato) Introduction Nowadays people are trying to stay away from everything synthetic, including preservatives,
More informationBiological Consulting Services of North Florida, Inc.
Biological Consulting Services of North Florida, Inc. June 29, 2015 Village Water Filters 1258 Rainbow Drive #2839 Silverthrone, CO 80498 RE: Biological filtration efficacy testing of the filters provided
More informationIndigo-Clean White Paper: #1 Bactericidal Performance Testing of Indigo-Clean Upon Bacterial Species. Healthcare
Indigo-Clean White Paper: #1 Bactericidal Performance Testing of Indigo-Clean Upon Bacterial Species Healthcare HC Bactericidal Performance Testing of Indigo-Clean Upon Bacterial Species Clifford J. Yahnke,
More informationCHAPTER 4 DISCUSSION. Many types of suitable media can be used to support the fungal growth and there is no
CHAPTER 4 DISCUSSION 4.1 Media Preparation and Subculture Many types of suitable media can be used to support the fungal growth and there is no specific medium ideally suited for the culture of species
More informationBiological Consulting Services of North Florida, Inc.
Biological Consulting Services of North Florida, Inc. January 5, 2016 Icon Lifesaver Ltd. Hall Chase, London Road Marks Tey, Colchester Co6 1EH, UK +44(0)1206 580999 Re: Biological filtration efficacy
More informationBiological Consulting Services of North Florida, Inc.
Biological Consulting Services of North Florida, Inc. January 5, 2016 Icon Lifesaver Ltd. Hall Chase, London Road Marks Tey, Colchester Co6 1EH, UK +44(0)1206 580999 Re: Biological filtration efficacy
More informationá62ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS
USP 40 Microbiological Tests / á62ñ Microbiological Examination 1 á62ñ MICROBIOLOGICAL EXAMINATION OF NONSTERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS INTRODUCTION The tests described hereafter
More informationThis study report is published with the express written consent of Antimicrobial Test Laboratories. Business Mobility Now!
mobile Antimicrobial Lab Test Report The Socket SoMo 650Rx handheld computer incorporates antimicrobial materials that provide an extra layer of protection to the device against the spread and multiplication
More informationSome Industrially Important Microbes and Their Products
2 Some Industrially Important Microbes and Their Products 2.1. ENZYME PRODUCING MICROBES Type of enzyme Substrate Microorganism Amylase Starch Saccharomyces diastaticus Protease Proteins Bacillus sp. Lipase
More informationAurachin SS, a new antibiotic from Streptomyces sp. NA04227
Supporting Information Aurachin SS, a new antibiotic from Streptomyces sp. NA04227 Mei Zhang, Cheng Long Yang, Yong Sheng Xiao, Bo Zhang, Xin Zhao Deng, Li Yang, Jing Shi, Yi Shuang Wang, Wei Li, Rui Hua
More informationCleaning Efficiency of The MicroOne Wipe and Mop Cleaning System
PDA Laboratories, Inc. A Contract ing Laboratory Visit us at www.pdalabsinc.com 17A Kiel Avenue Kinnelon, NJ 07405 (Tel) 973-492-8000 (Fax) 973-492-8002 pdainfo@pdalabsinc.com Cleaning Efficiency of The
More informationCONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS
CONTROL OF MICROBIAL GROWTH - DISINFECTANTS AND ANTISEPTICS Specific control measures can be used to kill or inhibit the growth of microorganisms. A procedure which leads to the death of cells is broadly
More informationMicrobiology sheet (6)
Microbiology sheet (6) Made by marah marahleh corrected by : abd. Salman DATE :9/10/2016 Microbial growth / control of microbial growth 1 The method of counting bacteria is divided into: 1) direct 2) indirect
More informationSerial dilution and colony count (Viable count) Pour plate. Spread plate Membrane filtration. Turbidity. Microscopic cell count
Aljawharah Alabbad 2016 Serial dilution and colony count (Viable count) Pour plate Spread plate Membrane filtration Turbidity Microscopic cell count Many studies require the quantitative determination
More information01/08/2018. Counting Microorganisms. Counting microorganisms. Turbidity Measurements. Relative abundance. Direct counts.
Counting Microorganisms 1 Counting microorganisms 2 Relative abundance Turbidity measurements Direct counts Absolute counts Viable counts Absolute number of growing bacteria Most probale number (MPN) Probable
More informationThe Efficacy of 300 ppm Peracetic Acid from Perasan MP-2 and MP-2C on E. Coli O157:H7 Inoculated Meat Surfaces
The Efficacy of 300 ppm Peracetic Acid from Perasan MP-2 and MP-2C on E. Coli O157:H7 Inoculated Meat Surfaces Background Joseph Donabed, B.S. Enviro Tech Chemical Services, Inc. October 25 th, 2016 Enviro
More informationAntimicrobial Lab Test Report
m o b i l e Antimicrobial Lab Test Report The Socket SoMo 650Rx handheld computer incorporates antimicrobial materials that provide an extra layer of protection to the device against the spread and multiplication
More informationMICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods
Issue No.29 / March 2010 MICROBIOLOGICAL TOOLS FOR QUALITY ASSURANCE IN HATCHERY: Laboratory Methods By Dr Vincent TURBLIN, Deputy Regional Market Manager Poultry - CEVA Animal Health Asia Pacific Most
More informationPotential MIC of bioactive peptides from fermented bovine milk to inhibit bacterial pathogens
International Journal of Advanced Multidisciplinary Research ISSN: 2393-8870 www.ijarm.com DOI: 10.22192/ijamr Volume 3, Issue 10-2016 Research Article Potential MIC of bioactive peptides from fermented
More informationGuidelines for Laboratory Verification of Performance of the FilmArray Blood Culture Identification (BCID) Panel
Guidelines for Laboratory Verification of Performance of the FilmArray Blood Culture Identification (BCID) Panel Purpose The Clinical Laboratory Improvement Amendments (CLIA), passed in 1988, establishes
More informationFinal text for addition to The International Pharmacopoeia
March 2012 3.3.2 MICROBIOLOGICAL EXAMINATION OF NON-STERILE PRODUCTS: TESTS FOR SPECIFIED MICROORGANISMS Final text for addition to The International Pharmacopoeia This monograph was adopted at the Forty-sixth
More informationProtocols for Laboratory Verification of Performance of the FilmArray Blood Culture Identification (BCID) Panel
Protocols for Laboratory Verification of Performance of the FilmArray Blood Culture Identification (BCID) Panel Purpose The Clinical Laboratory Improvement Amendments (CLIA), passed in 1988, establishes
More information2.1 Tryptone Soya Broth containing 4% Tween 80 (TSB + T), or another appropriate deactivating broth.
Prepared by: Date: Supersedes: Checked by: Date: Date Issued: Approved by: Date: Review Date: 1. SCOPE AND APPLICATION This general test method applies to the determination of microbiological test method
More informationLAB NOTES FOR EXAM 1 SECTION
LAB NOTES FOR EXAM 1 SECTION EX. 2-1: DIVERSITY AND UBIQUITY OF MICROOGANISMS Purpose: Microorganisms are found everywhere in the environment around us. To demonstrate this and to get a taste of the different
More information1. Procedure for Antibiotic susceptibility test by disc diffusion analysis
Nanoparticles Functionalized with Ampicillin Destroy Multiple Antibiotic Resistant Isolates of Pseudomonas aeruginosa, Enterobacter aerogenes and Methicillin Resistant Staphylococcus aureus Ashley Brown
More informationToday s Topics. General Quality Control Best Practices. Practices Antimicrobial Effectiveness Testing(AET) Best Practices Environmental Isolates
Laboratory Best Practices in QC Microbiology Today s Topics General Quality Control Best Practices Growth Promotion Testing (GPT) Best Practices Antimicrobial Effectiveness Testing(AET) Best Practices
More informationMIC & Etest. Dr. M. Talebi Ph.D of Bacteriology Tehran University of Medical Sciences
MIC & Etest Dr. M. Talebi Ph.D of Bacteriology Tehran University of Medical Sciences MIC The minimum inhibitory concentration (MIC) is defined as the lowest concentration of the antimicrobial agent required
More informationGROWTH AND SURVIVAL OF PATHOGENIC E. COLI DURING CURDLING OF MILK
Int. J. LifeSc. Bt & Pharm. Res. 2014 Aryya Mitra and Sanjib Ghoshal, 2014 Research Paper ISSN 2250-3137 www.ijlbpr.com Vol. 3, No. 1, January 2014 2014 IJLBPR. All Rights Reserved GROWTH AND SURVIVAL
More informationONAMER M. PRESERVATIVE and ANTIMICROBIAL ONAMER
ONAMER M PRESERVATIVE and ANTIMICROBIAL ONAMER M Stepan Lipid Nutrition is a division of Stepan Company which manufactures lipid and polymer based ingredients. HO OH SUMMARY Our quaternary ammonium polymer
More informationTransport of Bacteria on Sloping Soil Surfaces by Runoff
Transport of Bacteria on Sloping Soil Surfaces by Runoff Jamal Abu-Ashour, 1 Hung Lee 2 1 Department of Agricultural Engineering and Technology, Jordan University of Science and Technology, Irbid 22110,
More informationBD GeneOhm MRSA ACP Lysis Kit
REF 441638 100 Tests DOPS09-09-V1E1 USA Date: 2010-01 TABLE OF CONTENTS INTENDED USE...3 SUMMARY AND EXPLANATION OF THE PROCEDURE...3 REAGENTS...3 PRECAUTIONS...3 MATERIALS PROVIDED...4 STORAGE, HANDLING
More informationTest Method of Specified Requirements of Antibacterial Textiles for Medical Use FTTS-FA-002
Test Method of Specified Requirements of Antibacterial Textiles for Medical Use FTTS-FA-002 FTTS-FA-002 Antibacterial Textiles for Medical Use Antibacterial Textiles suppress and even kill harmful bacteria
More informationBIO & PHARMA ANALYTICAL TECHNIQUES
BIO & PHARMA ANALYTICAL TECHNIQUES Chapter 11 by Dr. Siti Umairah Mokhtar Faculty of Engineering Technology umairah@ump.edu.my Chapter Description Aims Discuss theory, principles and application of analytical
More informationFINAL REPORT STUDY TITLE
Final Report: Germicidal Spray Test of ZeroMold Plus Page 1 of 18 FINAL REPORT STUDY TITLE Germicidal Spray Test of ZeroMold Plus Disinfectant SOUTHWEST RESEARCH INSTITUTE PROJECT NUMBERS 01.19898.01.001
More informationProtocols for Laboratory Verification of Performance of the BioFire FilmArray Blood Culture Identification (BCID) Panel
Protocols for Laboratory Verification of Performance of the BioFire FilmArray Blood Culture Identification (BCID) Panel A Laboratory Protocol for Use with Live s Purpose The Clinical Laboratory Improvement
More informationINTRODUCTION water-soluble Figure 1.
INTRODUCTION Natural waters contain bacteria. The aerobic gram negative bacillus of the genera Psedomonas, Alcalignes, and Flavobacterium are common in natural waters. Many of these bacteria are able to
More information