Purification Expertise

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2 Purification Expertise Bench Polishing Pilot Production Scalable purification media: UHPLC HPLC Prep Silica, Polymer, and Hybrid Particle Technology Platforms Over 30 distinct stationary phase chemistries: for NP, RP, IEX, SEC, HILIC, Chiral Services available for Method Development, Purification Support, Training, Validation Support Audits welcome Phone Web

3 Kromasil EternityXT Designed for harsh conditions From UHPLC to industrial-scale HPLC Kromasil EternityXT is the latest extension of the Eternity chromatography stationary phases and columns portfolio, for separations and purifications that can be run under a wider range of ph conditions, with particle sizes between 1.8 and 10 µm. Learn more about EternityXT in 2 min: PREP 2016 Visit us at booth #209

4 ADVANCE BIOPHARMACEUTICALS Expert collaboration and J.T.Baker chemistries: helping you meet the unique challenges you face along every step of your biopharmaceutical production process U P S T R E A M D O W N S T R E A M Formulation Research and Development ULTRAPURE BIOREAGENTS and Biological Buffers Fermentation and Cell Growth J.T. Baker HPLE Sugars Additives: Amino Acids Minerals and Vitamins Sugars, Buffers and Antioxidants Product Harvest Denaturants and Detergents for Cell Disruption Product Purification J.T. Baker BAKERBOND Process Chromatography media Buffer salts Fill and Finish Bioexcipients Featuring J.T.Baker Process Chromatography media for Downstream Purification: BAKERBOND Mixed Mode BAKERBOND Multimode Ion Exchange BAKERBOND Ion Exchange BAKERBOND Silica Reversed Phase BAKERBOND Normal Phase Unique selectivity High linear throughput High capacity Learn more at

5 Trends & Developments in BioProcess Technology The BioProcessing Journal (BPJ) is a peerreviewed, quarterly publication that covers the latest technological advancements and best practices for the development and production of safe and effective biologics. Since 2002, BPJ has been a leading source of industry trends and techniques for process efficiency with its content specifically designed for professionals in process and analytical methods development, as well as manufacturing, quality systems, regulatory affairs, facility design, and contract services. Each article is carefully selected for its content and non-commercial nature, and reviewed by one of the best known and respected Editorial Advisory Boards in the industry. Open Access: Authors have the option of publishing their article with open access to everyone priced at $1,400 per manuscript. * DISCOVER irresistible pricing in advertising! Consult our Media Kit with Editorial Calendar when making your media choices. info@ bioprocessingjournal.com. SPEAKERS: Turn your presentation into an OPEN ACCESS Conference Exclusive article for publication in Contact us today! BioProcessing Journal 110 Willow Grove Ct., Norfolk VA USA Phone: info@bioprocessingjournal.com and editor@bioprocessingjournal.com Website: SPRING 2016 Volume 15/ Issue 1 ISSN BioProcessing J O U R N A L Trends & Developments in BioProcess Technology A Production of BioProcess Technology Network *

6 Working together to bring life science resources to your fingertips Access relevant information and articles from the industry s most respected authors and companies. americanpharmaceuticalreview.com pharmoutsourcing.com americanlaboratory.com

7 Reliable continuous chromatography. Our ÄKTA pcc 75 system. ÄKTA pcc 75 allows for purification of your target molecule in a continuous bioprocess. Productive by maximizing use of chromatography medium capacity Stable process performance even under varying conditions Scalable from process development to manufacturing scale gelifesciences.com/akta GE and GE monogram are trademarks of General Electric Company General Electric Company. First published Oct GE Healthcare Bio-Sciences AB, Björkgatan 30, Uppsala, Sweden AB 04/2016

8 Premier Training for Analytical Scientists Analytical Training Solutions is the leading global portal for fundamentals, best practice, troubleshooting and method development training for chromatographic and mass spectrometric techniques. Comprehensive, self-paced online courses and validated learning provides a unique educational resource for analytical scientists. Courses Available HPLC HPLC Basics, Equipment, and Troubleshooting Fundamentals of HPLC HPLC and UHPLC Troubleshooting: A Performance Qualification Approach Advanced HPLC Method Development Using Quality by Design Principles of HPLC Validation Practical HPLC for Biopharmaceuticals LC-MS LC-MS/MS for Chromatographers LC-MS Practitioner s Problem-Solving Toolkit Gas Chromatography Introduction to GC Intermediate GC GC-MS for Operators and Method Developers Method Quality Fundamentals of Quality Control with Control Charting Why ATS? Expert course presenters Validated learning & certification Comprehensive self-paced content RSC approved training courses Capillary Electrophoresis Introduction to Capillary Electrophoresis Capillary Electrophoresis Method Development Approved training Brought to you in collaboration with part of Eclipse Business Media Ltd

9 Welcome to PREP 2016 vibrant exhibit. On behalf of the Organizing Committee, I welcome you again to Philadelphia and to PREP 2016, the 29th International Symposium, Exhibit & Workshops on Preparative and Process Chromatography. PREP2016 continues the tradition of driving scientific progress with an exciting technical program, providing in-depth technical education and training with an extensive workshop program, and showcasing the latest commercial technology with The Scientific Program includes 73 oral presentations and 72 posters addressing the most recent developments in preparative chromatography from the gram scale to the multiple ton scale for both small molecules and biomolecules. The oral program includes: keynote sessions on Industrial Protein Chromatography (1), Continuous Chromatography (2), Preparative chromatography in Drug Discovery, Development, and Manufacture (5), Preparative Supercritical Fluid Chromatography (10), and Preparative Purification of Peptides (11); plenary sessions on Understanding and Modeling Biomolecules Chromatography (6), Innovative Materials and Processes for Biochromatography (7), Chromatography for Virus Particles (12), Chromatography Fundamentals (13), and Advances in CPC, CCC, and Chiral Separations (14); and parallel sessions on Strategies and Processes for Biomolecule Purification, (3A), Monoliths and Membrane Chromatography (4A), Stationary Phases (3B and 4B), New Developments in Affinity Chromatography (8A), New Developments and Applications of Continuous Chromatography (8B), and Column Characterization (9A), and Advances in Chromatographic Modalities for Bioprocess Applications (9B). Posters will be on display over a two-day period allowing you to interact extensively with the presenters. The restructured Training Workshop Program led by a team of internationally renowned experts includes Sunday Workshops on (1) Fundamentals of Preparative Chromatography for Biomolecule Purification by Batch and Continuous Processes, (2) Fundamentals of Preparative Chromatography for Purification of APIs, Peptides, and Oligonucleotides by Batch Chromatography, SMB, and SFC, and (3) Regulatory Fundamentals, QbD, and Design of Experiments for Biopharmaceuticals. In addition, there will be a totally new and exciting Monday Morning Tutorial on Tips, Tricks, and Troubleshooting Analytical and Overloaded Prep Chromatography. The Vendor Exhibit includes 23 booths through which you can become familiar with the latest commercial advances in preparative and process chromatography. Seven Vendor Workshops sponsored by AkzoNobel/Kromasil, Bio-Rad Laboratories, DAISO Fine Chem USA, GE Healthcare, Itochu Chemicals America/Mitsubishi Chemical, Purolite Life Sciences, and Thermo Fisher Scientific will complement the exhibit with more extensive information. These workshops are free and include breakfast or lunch, but you must register in advance by visiting the vendor booth. We invite to take advantage of all of these unique training and educational opportunities and to interact with vendors and providers of chromatography media, equipment, processes, and services. I wish to thank the many people who have helped make PREP 2016 possible, most continuing after many years of service but also many new people who have joined the PREP community. Firstly, I want to thank the members of the Scientific Advisory Committee and of the Industrial Advisory Committee for their help in planning and promoting the Symposium and reviewing the abstracts. Secondly, on behalf of all the participants, I want to thank our industrial sponsors, who have provided very generous financial support, the vendors, who have contributed to the exhibit and workshops, and all of the people who have submitted so many excellent abstracts. Thirdly, I want to thank our Symposium and Exhibit Manager, Ms. Janet Cunningham of Barr Enterprises, and her staff as well as several student aides who have volunteered to ensure a smooth running of the symposium. Finally, I want to thank you for attending PREP As we begin PREP 2016, we are already thinking about future PREP Symposia. The Organizers would absolutely welcome your comments and suggestions. While you attend PREP 2016, please take note and then let us know of what you see that could be improved, what new topics could be brought to the meeting, and what new formats might provide more effective exchanges of scientific ideas and awareness of new technologies. Your feedback and ideas would be highly appreciated. In the meanwhile, I very much hope you will enjoy this meeting and that the talks, posters, exhibits, training and vendor workshops, discussions, and networking opportunities will help you solve today s separation problems and better prepare you for the future of preparative chromatography. Giorgio Carta, University of Virginia PREP 2016 Chair PREP 2016 Final Program Page 1

10 PREP 2016 FINAL PROGRAM PREP 2016 Chair Giorgio Carta, University of Virginia Organizing Committee Lois Ann Beaver, LAB Enterprises Giorgio Carta, University of Virginia (Chair) Olivier Dapremont, AMPAC Fine Chemicals Igor Quinones-Garcia, Shire Qi (Tony) Yan, Pfizer Scientific Advisory Committee Dorota Antos, Rzeszow University of Technology, Poland Steven Cramer, Rensselaer Polytechnic Institute Attila Felinger, University of Pecs, Hungary Ranga Godavarti, Pfizer Kent Goklen, GlaxoSmithKline Art Hewig, Amgen Alois Jungbauer, BOKU, Vienna, Austria Abraham Lenhoff, University of Delaware Massimo Morbidelli, ETH Zurich, Switzerland David Robbins, MedImmune David Roush, Merck & Co., Inc. Melody Schmidt, Genentech Andreas Seidel-Morgenstern, Max-Planck Institute, Magdeburg, Germany Owen Thomas, University of Birmingham, UK Shuichi Yamamoto, Yamaguchi University, Japan Industrial Advisory Committee Jonathan Edelman, Washington Chromatography Discussion Group Marc Jacob, Phenomenex Mayumi Kiyono-Shimobe, Mitsubishi Chemical Corporation Jiali Liao, Bio-Rad Laboratories Ceclia Mazza, AkzoNobel/Kromasil Kathleen Mihlbachler, LEWA Process Technologies Ernie Sobkow, YMC America Eric Valery, Novasep Alla Zilberman, Semba Biosciences Poster Session Chair Dorota Antos, Rzeszow University of Technology, Poland Symposium / Exhibit Manager Ms. Janet Cunningham, Barr Enterprises Phone janet@barrconferences.com PREPsymposium.org Use of still or video cameras and cell phones is prohibited during the oral program; and prohibited in the poster and exhibition areas without the express consent of the presenter or exhibitor. Opinions expressed by presenters, instructors and exhibitors are not necessarily the opinions of the PREP 2016 Symposium. PREP 2016 Final Program Page 2

11 Table of Contents Page Welcome Message 1 Committees 2 Oral and Poster Session Presentation Guidance 4-5 Poster Competition 5 Corporate Sponsors and Media Partners 6 Conference History 6 General Information 7 Sunday Workshops and Monday Tutorial Program 8 Free Vendor Workshops 9 At-a-glance List of Sponsors, Exhibitors, Media 10 Scientific Program List of Exhibitors PREP 2016 Final Program Page 3

12 Oral and Poster Session Presentation Guidance AUTHOR INDEX The Author Index is located in the back of the Final Program book. "L" preceding the abstract number = Lecture "P" preceding the abstract number = Poster To locate your paper number, please refer to the Author Index located in the back of the Final Program Book. KEY TO First Symbol P = Poster Presentation P-M-200 POSTER BOARD L = Lecture Presentation L-M-100 & ABSTRACT NUMBERS Second Symbol Day to present poster P-T-200 M = Monday T = Tuesday Last Symbol Poster Presentation Number P-M-100 Lecture Presentation Number L-200 ORAL PRESENTERS Prior to the start of each session, please arrive at your session at least 20 minutes before the start of the session to introduce yourself to the session chair and to submit your presentation on a flash drive labeled with the presenter s name. Important to note that if there is no time to submit your presentation between sessions, please submit the presentation during the break that immediately precedes your session. Please come to the podium and get your presentation set up during the question period for the previous talk. Kindly note that session chairs are under very strict instructions to keep their sessions on schedule. POSTER PRESENTERS Details regarding set-up, presentation, and tear-down days and times: ALL posters in Poster Sessions I and II should be put up on Monday, July 18, between 8:30 AM and 1:00 PM and remain on the poster boards for participants to view both days (do not remove until Tuesday between 3:15 PM to 6:00 PM). Be sure to remove the reprint envelope where people leave business cards to request reprints of your abstract anything remaining after 6:00 PM will be discarded. Poster presentations are numbered in the scientific program to correspond with the poster board number. To verify your poster board number, please refer to the Author Index located in the back of the Final Program. Presenters must be in attendance at their posters on the day and time of their poster presentations. Authors of posters in the P-100 series should stand at their posters and be available to discuss the research during Poster Session I on Monday from 2:00 PM to 3:15 PM. Authors of posters in the P-200 series should stand at their posters and be available to discuss the research during Poster Session II on Tuesday from 2:00 PM to 3:15 PM. Remove all posters from the poster boards only on Tuesday between 3:15-6:00 PM. Anything remaining on the poster boards after 6:00 PM will be discarded. PREP 2016 Final Program Page 4

13 Poster Session Presentation Guidance POSTER SET UP ALL posters must be set up on Monday, between 8:30 AM to 1:00 PM. ALL posters stay up on the poster boards for two days (do NOT remove until the end of the day on Tuesday). POSTER TEAR DOWN Only remove posters from the poster boards on Tuesday between 3:15-6:00 PM. POSTER SESSIONS Posters are located in the Millennium Exhibit Hall, 2nd floor. Poster board numbers correspond to the poster presentation numbers in the Final Program. Authors presenting posters are required to be in attendance at their poster board during the Poster Session on the day/time of their poster presentation. Reprint envelopes are attached to the poster boards. To request reprints of poster abstracts, please insert your business card in the envelope. Each day, poster presenters should look in their reprint envelopes to retrieve any business cards that may be inside the envelope. Leave the poster on the poster board for 2 days; do not remove until Tuesday. Best Poster Competition POSTER COMPETITION Wednesday Poster presentations are a very important component of the PREP Symposia. In order to acknowledge their contribution to the field and high standards of the symposium, awards will be offered to distinguish the best poster contributions at PREP Posters will be evaluated on the basis of scientific content, clarity of presentation, and layout. Posters co-authored by members of the Scientific and Industrial Advisory Committees or by judges are eligible only if the main author and presenter of the poster is not a member on the above committees. Posters authored or co-authored by members of the Organizing Committee or judges are not eligible for Best Poster Awards. However, should these posters be considered of sufficient quality to be among the top prize-winning entries, they will be given an Honorable Mention. The Poster Judging Committee will have final say in the selection of the Prize Winners. At least two committee members will read each poster and top posters will be read by at least four committee members. If a poster author does not want his/her poster considered for a poster award, they must notify the Symposium Manager before 11:00 a.m. on Tuesday, July 19. Presentation of awards to winners of the Best Poster Competition will take place at the end of the Wednesday morning sessions at 12:30-12:40 PM. The winners are encouraged to be present, but it is not mandatory to be present to win. PREP 2016 Final Program Page 5

14 Thank you to our Corporate Sponsors Thank you to our Media Partners PREP Symposium Conference History 1985 Washington DC 2002 Washington DC 1986 Washington DC 2003 San Francisco, CA 1987 Washington DC 2004 Baltimore, MD 1989 Washington DC 2005 Philadelphia, PA 1991 Washington DC 2006 Washington DC 1993 Washington DC 2007 Washington DC 1994 Washington DC 2008 San Jose, CA 1995 Washington DC 2009 Philadelphia, PA 1996 Washington DC 2010 Philadelphia, PA 1997 Washington DC 2011 Cambridge, MA 1998 Washington DC 2012 Cambridge, MA 1999 San Francisco, CA 2013 Boston, MA 2000 Washington DC 2014 Boston, MA 2000 Washington DC 2015 Philadelphia, PA 2001 Washington DC 2016 Philadelphia, PA PREP 2016 Final Program Page 6

15 General Information VENUE Loews Philadelphia Hotel 1200 Market Street, Philadelphia, PA 19107, USA Phone NAME BADGES A name badge must be worn by each registered participant and accompanying person in order to gain admittance to the meeting, exhibit halls and social gatherings. You must wear your official conference name badge (no badge sharing), and your name and name badge must be visible at all times, in order to enter, and while you are inside, the meeting rooms and exhibition hall. Persons without a visible name badge, or with a badge that is not their own name badge, will be escorted out of the meeting room or exhibition hall. REGISTRATION Symposium Registration is located near Commonwealth Hall, 2nd floor EXHIBITS The Exhibition is an important component of the meeting, so please take the time to thank all the exhibitors for their support of the program by visiting the booths located in Millennium Hall, 2nd floor. EXHIBIT HOURS Sunday 6:00 PM 7:30 PM Monday 10:15 AM 5:15 PM Tuesday 9:20 AM 3:30 PM SOCIAL Sunday Welcome Reception 6:00 PM - 7:30 PM Millennium Hall, 2nd floor NETWORKING Monday Mixer 12:30 PM - 3:20 PM Millennium Hall, 2nd floor Tuesday Mixer 12:30 PM - 3:20 PM Millennium Hall, 2nd floor Wednesday Farewell Reception 4:50 PM - 5:50 PM Commonwealth Foyer, 2nd floor MESSAGE AND JOB POSTINGS BOARD The Message and Job Posting board is located on the 2nd floor between the Commonwealth and Millennium Halls. The use of still or video cameras and cell phones is prohibited during the oral program; and prohibited in the poster and exhibition areas without the express consent of the presenter or exhibitor. Opinions expressed by presenters, instructors and exhibitors are not necessarily the opinions of the PREP 2016 Symposium. You must wear your official conference name badge (no badge sharing), and your name and name badge must be completely visible at all times, in order to enter, and while you are inside, the meeting rooms and exhibition hall. Persons without a visible name badge, or with a badge that is not their own name badge, will be escorted out of the meeting room or exhibition hall. PREP 2016 Final Program Page 7

16 Sunday Workshops and Monday Tutorial Training Program Sunday, July 17 Workshops (separate registration fee) Instructors 9:00am-12:30pm Commonwealth Hall A1 Fundamentals of Preparative Chromatography for Biomolecule Purification by Batch and Continuous Processes Focus on biomolecule chromatography, stationary phases, binding capacity and selectivity, mass transfer, modeling, design for capture and resolution, multicolumn and continuous chromatography processes. 12:30pm-4:00pm Commonwealth Hall A2 Fundamentals of Preparative Chromatography for Purification of APIs, Peptides, and Oligonucleotides by Batch Chromatography, SMB, and SFC Focus on small molecule pharmaceuticals, APIs, chiral molecules, peptides, oligonucleotides, HPLC, column packing, gradient elution, overloaded chromatography, continuous chromatography, SMB, SFC, examples and industrial applications. Regulatory Fundamentals, QbD, and Design of Experiments for 4:00pm-6:30pm Commonwealth Hall A1 Biopharmaceuticals Focus on regulatory aspects, QbD/DOE principles and practical examples, Process Analytical Technology, Quality System Management, biosimilars. Monday, July 18 Tutorial (separate registration fee) Instructors Tips, Tricks, and Troubleshooting Analytical and Overloaded Prep 7:00am-8:30am Chromatography Commonwealth Focus on analytical chromatography, Hall A1 overloaded chromatography, HPLC, SFC, examples of small molecules, APIs, peptides. Giorgio Carta, University of Virginia Alan Hunter, MedImmune Alois Jungbauer, BOKU, Vienna Massimo Morbidelli, ETH Zurich Olivier Dapremont, Ampac Fine Chemicals Lois Ann Beaver, LAB Enterprises Gisela Ferreira, MedImmune Cecilia Mazza, AkzoNobel Tony Yan, Pfizer, Inc. PREP 2016 Final Program Page 8

17 Monday Free Vendor Workshops must pre-register on Monday by 10:50 AM Monday Free Vendor Workshop sponsored by Purolite Life Sciences 12:45-2:00 PM A Bead for Every Need, from MAb Purification to Uranium Extraction Location: Commonwealth Hall B, 2nd floor (light lunch will be provided) Must pre-register at the booth of Purolite by 10:50 AM Monday Free Vendor Workshop sponsored by Bio-Rad Laboratories 12:45-2:00 PM Utilizing Novel Mixed-Mode Selectivities for Challenging Chromatography Separations Location: Commonwealth Hall A1, 2nd floor (light lunch will be provided) Must pre-register at the booth of Bio-Rad Laboratories by 10:50 AM Monday Free Vendor Workshop sponsored by DAISO Fine Chem USA, Inc. 12:45-2:00 PM Re-evaluation of the CIP Step in Insulin-related RP HPLC Processes Location: Commonwealth Hall A2, 2nd floor (light lunch will be provided) Must pre-register at the booth of DAISO Fine Chem USA by 10:50 AM Tuesday Free Vendor Workshops must pre-register on Monday by 5:00 PM Tuesday Free Vendor Workshop sponsored by AkzoNobel/Kromasil 12:45-2:00 PM Latest Developments of Stationary Phases for Preparative Chromatography Location: Commonwealth A1, 2nd floor (light lunch will be provided) Must pre-register at the booth of AkzoNobel/Kromasil by 5:00 PM Tuesday Free Vendor Workshop sponsored by Thermo Fisher Scientific 12:45-2:00 PM Enabling Custom Solutions for Downstream Processing for Future Therapies Location: Commonwealth A2, 2nd floor (light lunch will be provided) Must pre-register at the booth of Thermo Fisher Scientific by 5:00 PM Tuesday Free Vendor Workshop sponsored by Itochu Chemicals America/Mitsubishi Chemical 12:45-2:00 PM Bioseparation Media by Mitsubishi Chemical Location: Commonwealth Hall B, 2nd floor (light lunch will be provided) Must pre-register at the booth of Itochu Chemicals America by 5:00 PM Tuesday Free Vendor Workshop sponsored by GE Healthcare Life Sciences 12:45-2:00 PM Affinity Chromatography Protein A Improvements and Beyond Location: Tubman Room, must take elevator to 3rd floor (light lunch will be provided) Must pre-register at GE Healthcare's Poster # P-T-218 by 5:00 PM PREP 2016 Final Program Page 9

18 At-a-glance List of Sponsors, Exhibitors, Media Partners Agilent Technologies AkzoNobel/Kromasil American Laboratory American Pharmaceutical Review Ampac Fine Chemicals Avantor Performance Materials Bioanalysis-Zone BioProcessing Journal Bio-Rad Laboratories Bristol-Myers Squibb CC Biotech DAISO Fine Chem USA Essential Life Solutions & emp Biotech GE Healthcare Life Sciences Genentech GENews GlaxoSmithKline International LabMate/Chromatography Today ITOCHU Chemicals America JASCO JSR Life Sciences Kaneka Lab Manager Magazine LabCompare LCGC MedImmune Merck NOVASEP Pfizer PIC Solution Purolite Life Sciences Quantum Analytics Semba Biosciences Separation Methods Technologies Separation Science Shire SP Scientific-Genevac Thermo Fisher Scientific YMC America YPSO-FACTO JNC America PREP 2016 Final Program Page 10

19 Sunday, July 17, :00 AM - 12:30 PM Workshop 1 on Fundamentals of Preparative Chromatography for Commonwealth A1 Biomolecule Purification by Batch and Continuous Processes Must pre-register to attend 12:30 PM - 4:00 PM Workshop 2 on Fundamentals of Preparative Chromatography for Commonwealth A2 Purification of APIs, Peptides, and Oligonucleotides by Batch Chromatography, SMB, and SFC Must pre-register to attend 4:00 PM - 6:30 PM Workshop 3 on Regulatory Fundamentals, QbD, and Design of Commonwealth A1 Experiments for Biopharmaceuticals Must pre-register to attend 1:00-5:30 PM Registration for Exhibitors Only to set up booth Location: Commonwealth Hall Foyer, 2nd floor 6:00-7:30 PM Symposium Registration Open for Conferees Location: Commonwealth Hall Foyer, 2nd floor 6:00-7:30 PM Grand Opening of the Exhibition & Welcome Reception Location: Millennium Hall, 2nd floor Open to all conference participants; conference name badge is required for entry. Monday, July 18, :00 AM - 8:30 AM Tutorial on Tips, Tricks, and Troubleshooting Analytical and Commonwealth A1 Overloaded Prep Chromatography Must pre-register to attend 7:45 AM - 5:15 PM Symposium Registration Open Location: Commonwealth Hall Foyer, 2nd floor 10:00 AM - 5:15 PM Exhibition Open in Millennium Hall, 2nd floor 8:30 AM WELCOME AND OPENING REMARKS Giorgio Carta, University of Virginia, Charlottesville, VA, USA Location: Commonwealth Hall C/D, 2nd floor 1. Monday Keynote: Industrial Case Studies in Protein Chromatography Session Chairs: Giorgio Carta, University of Virginia, Alan Hunter, MedImmune, Gaithersburg, MD, USA Location: Commonwealth Hall C/D, 2nd floor 8:40 AM (L-101) Development of a Semi-continuous, Integrated Chromatography Purification Platform for Perfusion and High Titer Fed Batch Antibody Production. Rebecca Chmielowski, Darshini Shah, Collette Cutler, Hong Li, David Roush, Nihal Tugcu, Merck, Kenilworth, NJ, USA 9:00 AM (L-102) Mechanistic Investigation of Aggregate Formation in CEX and Mitigation Strategy for an Unstable Fc-fusion Protein. Zhiqiang Chen, Chao Huang, Naresh Chennamsetty, Xuankuo Xu, ZhengJian Li, Bristol-Myers Squibb, Devens, MA, USA PREP 2016 Final Program 11

20 Monday, July 18, :20 AM (L-103) A New Approach to Assess the Process Clearance Capability for Critical Quality Attributes. Kathlyn Lazzareschi, Josefine Persson, Genentech A member of Roche, South San Francisco, CA, USA 9:40 AM (L-104) High Resolution Preparative Protein Separation using Novel ph Gradients Employing Simple Buffer Systems. Matthew Herigstad, Lihua Yang, Natarajan Ramasubramanyan, AbbVie, Westborough, MA, USA 10:00 AM (L-105) Purification of Recombinant Polyclonal Antibody (rpab) Mixtures: Impact of Polishing Modality on Aggregate Selectivity and mab Component Ratios. Alan Hunter, Frank Bartnik, Hongji Liu, Xiangyang Wang, Timothy Pabst, MedImmune, Gaithersburg, MD, USA 10:20-10:50 AM Break in Millennium Exhibit Hall, 2nd floor 2. Monday Keynote: Continuous Chromatography Session Chairs: Kathleen Mihlbachler, LEWA Process Technologies, Devens, MA, USA, Olivier Dapremont, AMPAC Fine Chemicals, Rancho Cordova, CA, USA Location: Commonwealth Hall C/D, 2nd floor 10:50 AM (L-106) Continuous Multicolumn Purification of Therapeutic Proteins. Fabian Steinebach, Daniel Baur, Nicole Ulmer, Lara Decker, Massimo Morbidelli, ETH Zurich, Zurich, SWITZERLAND 11:10 AM (L-107) Continuous Purification Platforms for Viral Vectors based on Multicolumn Simulated Countercurrent Chromatography. Ricardo Silva 1, Cristina Peixoto 1, Manuel Carrondo 1, Jose Mota 2, 1 IBET, Oeiras, PORTUGAL; 2 FCT-UNL & IBET, Caparica, PORTUGAL 11:30 AM (L-108) Model-based Conversion of a Single-column Batch Process to 3- and 4- Column Periodic Counter-current Chromatography. Tobias Hahn, Thiemo Huuk, Fabian Gorlich, Gang Wang, Jurgen Hubbuch, KIT, Karlsruhe, GERMANY 11:50 AM (L-109) Model Predictive Control of 4-zone Simulated Moving Bed Chromatography using a Decoupled and Simplified Process Model. Ju Weon Lee, Andreas Seidel- Morgenstern, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, GERMANY 12:10-12:30 PM (L-110) A Rationale Approach for Comparing Different Affinity Chromatographic Processes for the Capture of a Monoclonal Antibody. Roger-Marc Nicoud 1, Laurent David 1, Margit Holzer 1, Jay Yun 2, 1 Ypso-Facto, Nancy, FRANCE; 2 Ypso-Facto, Cambridge, MA, USA Monday Mixer in the Exhibition Hall Location: Millennium Hall, 2nd floor - Mixer includes light lunch 12:30-3:20 PM Break, Exhibits, Posters PREP 2016 Final Program 12

21 Monday, July 18, 2016 Monday Free Vendor Workshops Must pre-register at the sponsor's booth to attend; light lunch will be provided 12:45-2:00 PM A Bead for Every Need, from MAb Purification to Uranium Extraction, sponsored by Purolite Life Sciences Location: Commonwealth Hall B, 2nd floor (light lunch will be provided) Must pre-register at the booth of Purolite Life Sciences by 10:50 AM 12:45-2:00 PM Utilizing Novel Mixed-Mode Selectivities for Challenging Chromatography Separations, sponsored by Bio-Rad Laboratories Location: Commonwealth Hall A1, 2nd floor (light lunch will be provided) Must pre-register at the booth of Bio-Rad Laboratories by 10:50 AM 12:45-2:00 PM Re-evaluation of the CIP Step in Insulin-related RP HPLC Processes, sponsored by DAISO Fine Chem USA, Inc. Location: Commonwealth Hall A2, 2nd floor (light lunch will be provided) Must pre-register at the booth of DAISO Fine Chem USA by 10:50 AM MONDAY POSTER SESSION I Poster Session Chair: Dorota Antos, Rzeszow University of Technology, Poland Location: Millennium Exhibition Hall, 2nd floor 2:00-3:15 PM POSTER SESSION I - Sponsored by Bristol-Myers Squibb Location: Millennium Exhibit Hall, 2nd floor 3A. Monday Parallel Session: Strategies and Processes for Biomolecule Purification Session Chair: Abraham Lenhoff, University of Delaware, Newark, DE, USA Location: Commonwealth Hall C/D, 2nd floor 3:20 PM (L-111) Purification of Secretory Immunoglobulins by Chromatographic Methods. Hannah Engelmaier, Alexander Matlschweiger, Rainer Hahn, BOKU Vienna, Vienna, AUSTRIA 3:40 PM (L-112) Resolution of Positional Isomers of Exenatide Mono-PEGylates by Prep Chromatography. Thi Ngoc Thanh Nguyen, Soi Yoon, E. K. Lee, Hanyang University, Ansan, SOUTH KOREA 4:00 PM (L-113) Heat Precipitation of Tobacco Host Cell Proteins Facilitates Target Protein Recovery and Purification and Can be Implemented into an Automated Large-scale Manufacturing Process. Stephan Menzel, Rainer Fischer, Johannes Buyel, Fraunhofer IME, Aachen, GERMANY 4:20 PM (L-114) Optimizing Recombinant F(ab)2 Purification for Improved PET Imaging of Cancer Biomarkers. Mark Fitchmun 1, Kimberly Fitchmun 1, Ritsuko Sawada 2, Wolfgang Scholz 2, 1 Somatek Inc., San Diego, CA, USA; 2 MabVax Therapeutics, San Diego, CA, USA 4:40-5:10 PM Break in Millennium Exhibit Hall, 2nd floor PREP 2016 Final Program 13

22 Monday, July 18, B. Monday Parallel Session: Stationary Phases 1 Session Chairs: Shuichi Yamamoto, Yamaguchi University, Japan and Conan Fee, Canterbury University, New Zealand Location: Commonwealth Hall B, 2nd floor 3:20 PM (L-115) High Performance, Rapid Separation, and High Loading Capacity of the Novel Stationary Phase using Dual-pore Silica Beads in Reversed Phase Liquid Chromatography. Hong-zhi Bai 1, Riichi Miyamoto 1, Akie Kawamura 2, Mika Watanabe 2, Teruhiko Kanno 2, Takashi Ohtani 2, Kazuki Nakanishi 3, 1 SnG Inc., Kyoto, JAPAN; 2 Hamari Chemicals Ltd., Osaka, JAPAN; 3 Kyoto University, Kyoto, JAPAN 3:40 PM (L-116) Design and Evaluation of Advanced Amsphere A3 Protein A Chromatography Resin: The Relationships and Considerations of Bead structure, Pore Structure, Surface Chemistries and Ligand Design on Affinity Resin Performance Targets. Satoshi Nakamura 1, Alpana Naresh 2, Tomonori Shiotani 1, Marty Siwak 2, 1 JSR Micro, Durham, NC, USA; 2 JSR Micro, Sunnyvale, CA, USA 4:00 PM (L-117) Reducing Cost of Clinical Trials through the use of Novel Alkali Stable Protein A Resins. Hans Berg 1, Mark Hicks 2, Caroline Tinsley 2, Patrick Gilbert 2, Charlotte Vassay-Jones 2, Hans J. Johansson 1, 1 Purolite, Uppsala, SWEDEN; 2 Purolite, Llantrisant, WALES 4:20 PM (L-118) Single and Multi-component IgG Adsorption on Protein A Chromatography Resins. Justin Weinberg 1, Shaojie Zhang 2, Giorgio Carta 2, Todd Przybycien 1, 1 Carnegie Mellon University, Pittsburgh, PA, USA; 2 University of Virginia, Charlottesville, VA, USA 4:40-5:10 PM Break in Millennium Exhibit Hall, 2nd floor 4A. Monday Parallel Session: Monoliths and Membrane Chromatography Session Chair: Ales Podgornik, COBIK, Ljubljana, Slovenia Location: Commonwealth Hall C/D, 2nd floor (L-119) withdrawn 5:10 PM (L-120) Rapid Protein Ion-exchange Separations using Surface Modified Nylon Capillary-channeled Polymer (C-CP) Fibers. Liuwei Jiang, R. Kenneth Marcus, Clemson University, Clemson, SC, USA 5:30 PM (L-121) Optimization of Continuous Countercurrent Tangential Chromatography (CCTC) for Continuous Purification of Monoclonal Antibodies. Oleg Shinkazh 1, Andrew Zydney 2, 1 ChromaTan, State College, PA, USA; 2 Penn State University, State College, PA, USA 5:50 PM Pause PREP 2016 Final Program 14

23 Monday, July 18, B. Monday Parallel Session: Stationary Phases 2 Session Chairs: Shuichi Yamamoto, Yamaguchi University, Japan and Conan Fee, Canterbury University, New Zealand Location: Commonwealth Hall B, 2nd floor 5:10 PM (L-122) Mixed Mode Chromatography in Non-affinity based Purification Schemes. Carsten Voss, Bio-Rad Laboratories GmbH, Munich, GERMANY (L-123) withdrawn 5:30 PM (see also poster P-T-220) Adsorption and Separation of Native and PEGylated Proteins on Anion Exchange Resins with Varying Length of Polymer Grafting. Mimi Zhu, Preston Fuks, Giorgio Carta, University of Virginia, Charlottesville, VA, USA 5:50 PM (L-124) Dual Flow Chromatography for Parallel, Automated, High Throughput Process Development. Douglas Gjerde, Christopher Suh, Lee Hoang, PhyNexus Inc., San Jose, CA, USA 6:10 PM Pause Tuesday, July 19, :30 AM - 5:15 PM Symposium Registration Open Location: Commonwealth Hall Foyer, 2nd floor 9:00 AM - 3:30 PM Exhibition Open in Millennium Hall, 2nd floor 5. Tuesday Keynote: Preparative Chromatography in Drug Discovery, Development, and Manufacture Session Chair: Tony Yan, Pfizer, Groton, CT, USA Location: Commonwealth Hall C/D, 2nd floor 8:00 AM (L-201) Liquid Carbon Dioxide Flash: Has the Myth Become Reality. Ray McClain, Merck, West Point, PA, USA 8:20 AM (L-202) Design (and Avoidance) of Process-scale Chromatography through the use of Statistical Design of Experiments. Teri Hochdorfer, Fangfang Liu, Ke Wang, Glenn Wilcox, Pfizer, Groton, CT, USA 8:40 AM (L-203) Dual Purpose Analytical SFC/UHPLC Instrument with On-line SFE Capability for Automated Method Screening of Chiral and Achiral Separations. Robert Buco 1, Masayuki Nishimura 2, Yosuke Iwata 3, Kenichiro Tanaka 4, 1 Shimadzu Corporation, Marlborough, MA, USA; 2 Shimadzu Corporation, Columbia, MD, USA; 3 Shimadzu Corporation, Kyoto, JAPAN; 4 Shimadzu Scientific Instruments Inc., Columbia, MD, USA 9:00 AM (L-204) Chromatography as the Multi-tool of Drug Development and its Preparative Chiral Attachment. J. Preston, Michael McCoy, Phenomenex, Torrance, CA, USA 9:20-9:50 AM Break in Millennium Exhibit Hall, 2nd floor PREP 2016 Final Program 15

24 Tuesday, July 19, Tuesday Session: Understanding and Modeling Biomolecule Chromatography Session Chair: David Roush, Merck & Co., Inc., Kenilworth, NJ, USA Location: Commonwealth Hall C/D, 2nd floor 9:50 AM (L-205) Molecular Modeling Technique for Protein-surface Binding Free Energy Calculation and Prediction of Elution Behavior in Multimodal Chromatography. Suvrajit Banerjee, Shekhar Garde, Steven Cramer, Rensselaer Polytechnic Institute, Troy, NY, USA 10:10 AM (L-206) Probing Nanoscale Effects of Ligand Density in Tentacular Ion Exchangers using X-ray Scattering. Rahul Bhambure 1, Daniel Greene 1, Yun Liu 1, Christopher Gillespie 2, Michael Phillips 2, Heiner Graalfs 3, Almut Rapp 3, Abraham Lenhoff 1, 1 University of Delaware, Newark, DE, USA; 2 MilliporeSigma, Bedford, MA, USA; 3 Merck Millipore, Darmstadt, GERMANY 10:30 AM (L-207) Effect of Isotherm Nonlinearity on Protein Binding and Elution in Hydrophobic Interaction and Multimodal Chromatography. Renata Muca, Wojciech Piatkowski, Dorota Antos, Rzeszow University of Technology, Rzeszow, POLAND 10:50 AM (L-208) Influence of Mixed Electrolytes and ph on Adsorption of Proteins in Hydrophobic Interaction Chromatography. Eva Hackemann, Hans Hasse, University of Kaiserslautern, Kaiserslautern, GERMANY 7. Tuesday Session: Innovative Materials and Processes for Biochromatography Session Chair: Kathlyn Lazzareschi, Genentech, South San Francisco, CA, USA Location: Commonwealth Hall C/D, 2nd floor 11:10 AM (L-209) Fluid Dynamics Simulation based on 3D pore Structure of Macro Porous Monoliths Shows High Permeability Due to Alternating Channel Width. Alois Jungbauer 1, Christian Jungreuthmayer 2, Petra Gerster 1, 1 BOKU, Vienna, AUSTRIA; 2 acib, Vienna, AUSTRIA 11:30 AM (L-210) Optimization of Porous Bed Geometric Features to Maximize Adsorption of Proteins and Passage of Suspended Solids in a 3D-printed Adsorption Column. Conan Fee 1, Felix Newberry 1, Anne Gordon 1, Philip Wilson 1, Miguel Moyers-Gonzalez 1, Rua Murray 1, Tim Huber 1, Simone Dimartino 2, 1 University of Canterbury, Christchurch, NEW ZEALAND; 2 University of Edinburgh, Edinburgh, SCOTLAND 11:50 AM (L-211) A Scaleable Integrated System for Temperature-controlled Fast Protein Liquid Chromatography. Owen R.T. Thomas 1, Eirini Theodosiou 2, Matthias Franzreb 3, 1 University of Birmingham, Birmingham, UK; 2 Aston University, Birmingham, UK; 3 Karlsruhe Institute of Technology, Eggenstein-Leopoldshafen, GERMANY 12:10-12:30 PM (L-212) Modeling of Linear ph and Salt Gradient Elution on Weak Cation Exchange Resins. Felix Wittkopp, Christian Frech, University of Applied Sciences, Mannheim, GERMANY PREP 2016 Final Program 16

25 Tuesday, July 19, 2016 Tuesday Mixer in the Exhibition Hall Location: Millennium Hall, 2nd floor -- Mixer includes light lunch 12:30-3:20 PM Break, Exhibits, Posters Tuesday Free Vendor Workshops Must pre-register at the sponsor's booth to attend; light lunch will be provided 12:45-2:00 PM Latest Developments of Stationary Phases for Preparative Chromatography, sponsored by AkzoNobel/Kromasil Location: Commonwealth A1, 2nd floor (light lunch will be provided) Must pre-register at the booth of AkzoNobel/Kromasil by 5:00 PM 12:45-2:00 PM Enabling Custom Solutions for Downstream Processing for Future Therapies, sponsored by Thermo Fisher Scientific Location: Commonwealth A2, 2nd floor (light lunch will be provided) Must pre-register at the booth of Thermo Fisher Scientific by 5:00 PM 12:45-2:00 PM Bioseparation Media by Mitsubishi Chemical, sponsored by Itochu Chemicals America/Mitsubishi Chemical Location: Commonwealth Hall B, 2nd floor (light lunch will be provided) Must pre-register at the booth of Itochu Chemicals America by 5:00 PM 12:45-2:00 PM Affinity Chromatography Protein A Improvements and Beyond, sponsored by GE Healthcare Life Sciences Location: Tubman Room, must take elevator to 3rd floor (light lunch will be provided) Must pre-register at GE Healthcare's Poster # P-T-218 by 5:00 PM TUESDAY POSTER SESSION II Poster Session Chair: Dorota Antos, Rzeszow University of Technology, Poland Location: Millennium Exhibition Hall, 2nd floor 2:00-3:15 PM POSTER SESSION II - Sponsored by Bristol-Myers Squibb Location: Millennium Exhibit Hall, 2nd floor 8A. Tuesday Parallel Session: New Developments in Affinity Chromatography Session Chair: Rainer Hahn, BOKU, Vienna, Austria Location: Commonwealth Hall C/D, 2nd floor 3:20 PM (L-213) Making Protein A Media Stealthy. Todd Przybycien, Justin Weinberg, Carnegie Mellon University, Pittsburgh, PA, USA (L-214) withdrawn 3:40 PM (L-216) Development of a Continuous Protein A Capture Unit Operation: How Resin Characteristics Change the Multi-column Process. Nicolas-Julian Hilbold 1, Xavier Le Saout 2, Eric Valery 3, Jonathan Souquet 2, Laurence Muhr 4, 1 Novasep, Pompey, FRANCE; 2 Merck KGaA, Corsier-sur-Vevey, SWITZERLAND; 3 Novasep, Lyon, FRANCE; 4 LRGP (Reactions and Process Engineering Laboratory), Nancy, FRANCE 4:00-4:20 PM (L-215) A Platform for the Development of Affinity Peptide Resins for Capture and Downstream Purification of Non-mAb Biologics. Divya Chandra, Steve Timmick, Chaz Goodwine, Nick Vecchiarello, Pankaj Karande, Steven Cramer, Chemical & Biological Engineering Rensselaer Polytechnic Institute, Troy, NY, USA PREP 2016 Final Program 17

26 Tuesday, July 19, B. Tuesday Parallel Session: New Developments and Applications of Continuous Chromatography Session Chair: Attila Felinger, University of Pecs, Hungary Location: Commonwealth Hall B, 2nd floor 3:20 PM (L-217) Demonstration of a mab Chromatography Platform Process Operating in a Continuous and Integrated Mode: Flexibility for Facility Fit and Process Economics Optimization. Xhorxhi Gjoka, Rene Gantier, Mark Schofield, Pall Life Sciences, Westborough, MA, USA 3:40 PM (L-218) Separation of Astaxanthin from the Crude Extract of Haematococcus Pluvialis by Simulated Moving Bed. Xiao-Qing Bao, Wen-Tung Wu, Chih-Hsiung Lin, Ya-Ting Shih, Ming-Tsai Liang, I-Shou University, Kaohsiung, TAIWAN 4:00 PM (L-219) Large Scale (Ton Scale) Purification of Plant Derived Proteins using Simulated Moving Bed Chromatography. Anil Oroskar, Pravin Ninanwe, Babu Antharawalli, Priyanka Oroskar, Orochem Technologies Inc., Naperville, IL, USA 4:20-4:40 PM (L-220) An Integrated UV-VIS Detection Module for MCC in Achiral Separations. Gary Yanik 1, Leo Hsu 2, Xiqin Yang 2, 1 PDR-Separations, Palm Beach Gardens, FL, USA; 2 GlaxoSmithKline, King of Prussia, PA, USA 9A. Tuesday Parallel Session: Column Characterization Session Chair: Igor Quinones-Garcia, Shire, Lexington, MA, USA Location: Commonwealth Hall C/D, 2nd floor 4:50 PM (L-221) Measurement and Modeling of Extra Column Volume and System Band Broadening for Scale-up of Preparative Chromatography Columns. Susanne Schweiger 1, Christian Jungreuthmayer 1, Tim Schröder 2, Alois Jungbauer 3, 1 Austrian Centre of Industrial Biotechnology, Vienna, AUSTRIA; 2 Atoll GmbH, Weingarten, GERMANY; 3 University of Natural Resources and Life Sciences, Vienna, AUSTRIA 5:10 PM (L-222) Characterization and Application of Microliter Disposable Monolithic Columns (DMC) for PAT. Tanja Lukan 1, Shuichi Yamamoto 2, Noriko Yoshimoto 3, Ales Podgornik 1, 1 COBIK, Ljubljana, SLOVENIA; 2 Yamaguchi University, Ube, JAPAN; 3 Ube, JAPAN 5:30 PM (L-223) Chromatographic Column Transition Analysis using Moment Analysis. Brian Youchak 1, Willis Bell 2, 1 Eli Lilly and Company, Branchburg, NJ, USA; 2 Eli Lilly and Company, Indianapolis, IN, USA 5:50 PM (L-224) Planning the Execution of a Viral Clearance Study: Right First Time. Douglas Rea, Katherine Bergmann, Eurofins Lancaster Labs, Lancaster, PA, USA 6:10 PM Pause PREP 2016 Final Program 18

27 Tuesday, July 19, B. Tuesday Parallel Session: Advances in Chromatographic Modalities for Bioprocess Applications Session Chair: Christian Frech, Mannheim University of Applied Sciences, Germany Location: Commonwealth Hall B, 2nd floor 4:50 PM (L-225) Flow Through Chromatography and Absorptive Depth Filtration for Continuous Bioprocessing Applications Case Studies. Romas Skudas, Paul Beckett, Merck KGaA, Darmstadt, GERMANY 5:10 PM (L-226) SMART Chromatography - A Method for Packed Bed Purification from Cell Containing Feedstreams. Alistair Hurst, Derek Levison, Ruediger Welz, Franziska Meier-Haettig, emp Biotech GmbH, Berlin, GERMANY 5:30 PM (L-227) Purification of Acidic Proteins with Ceramic Hydroxyapatite: Bind-and- Elute Mode or Flow-through Mode? Xuemei He, Louisa Vang, Bio-Rad Laboratories, Hercules, CA, USA 5:50 PM (L-228) Bridging the Gap Between Analytical and Process Protein A Chromatography. J. Kevin O'Donnell 1, Atis Chakrabarti 1, William Evans 1, Kosuke Araki 2, Shigeru Nakatani 2, 1 Tosoh Bioscience LLC, King of Prussia, PA, USA; 2 Tosoh Corporation, Shunan, JAPAN 6:10 PM Pause 7:30 AM Symposium Registration Open Wednesday, July 20, Wednesday Keynote: Preparative Supercritical Fluid Chromatography (SFC) Session Chairs: Lois Beaver, LAB Enterprises, Chevy Chase, MD, USA and Jonathan Edelman, Washington Chromatography Discussion Group Location: Commonwealth Hall A/B, 2nd floor 8:00 AM (L-301) Operating Large Diameter Columns in Liquid and Supercritical Fluid Chromatography under Strictly Adiabatic Thermal Environment. Fabrice Gritti, Joseph Jarrell, Martin Gilar, Waters Corporation, Milford, MA, USA 8:20 AM (L-302) Stationary Phase Selection for Optimized Preparative SFC Chromatography. Matthew Przybyciel, ES Industries, West Berlin, NJ, USA 8:40 AM (L-303) Advances in Preparative Gas-expanded Liquid Chromatography. Jerome Boni 1, Celine Morey 1, Jin Seok Hur 2, Yvan Ruland 3, 1 Novasep, Pompey, FRANCE; 2 Novasep, Boothwyn, PA, USA; 3 Novasep, Shanghai, CHINA 9:00 AM (L-304) Reliable Scale-up from Linear Gradient Screening to Isocratic Bulkpurification in SFC. Jason Hill, Abhijit Tarafder, Waters, Milford, MA, USA 9:20-9:40 AM (L-305) Peak Distortions in Supercritical Fluid Chromatography due to Adsorption of the Co-solvent. Torgny Fornstedt 1, Emelie Glenne 1, Jörgen Samuelsson 1, Hanna Leek 2, Kristina Ohlen 2, Magnus Klarqvist 2, 1 Karlstad University, Karlstad, SWEDEN; 2 AstraZeneca R&D Molndal, Molndal, Sweden PREP 2016 Final Program 19

28 Wednesday, July 20, Wednesday Keynote: Preparative Purification of Peptides Session Chairs: Olivier Dapremont, AMPAC Fine Chemicals, Rancho Cordova, CA, USA, Marc Jacob, Phenomenex, Torrance, CA, USA Location: Commonwealth Hall A/B, 2nd floor 9:40 AM (L-306) State-of-the-Art Purification of Peptide APIs: Challenges and Achievements. Georg M. Sambeth 1, Günther Loidl 2, Ralph Schönleber 1, 1 Bachem AG, Bubendorf, SWITZERLAND; 2 Bachem Holding, Bubendorf, SWITZERLAND 10:00 AM (L-307) Chemically Stable Merged Organic/Inorganic Silica Material for Purifications of Peptides and Proteins in Reversed Phase Preparative Chromatography. Fredrik Lime, Cecilia Mazza, AkzoNobel/Kromasil, Bohus, SWEDEN 10:20 AM (L-308) Influence of Column Overloading on Selectivity in Peptide Purification. Guido Krautz 1, Tivadar Farkas 2, Marc Jacob 2, 1 Phenomenex Ltd., Aschaffenburg, GERMANY; 2 Phenomenex Inc., Torrance, CA, USA 10:40-10:50 AM Presentation of Awards to Winners of the Best Poster Competition 10:50-11:10 AM Break in Commonwealth Foyer, 2nd floor 12. Wednesday Session: Chromatography for Virus Particles Session Chair: Alois Jungbauer, BOKU, Vienna, Austria Location: Commonwealth Hall A/B, 2nd floor 11:10 AM (L-309) Combining Solute Surface Analytics and High Throughput Screening to Define Binding Mechanisms of Virus Particles to Multimodal Anion Exchange Resin. Matthew Brown 1, Sarah Johnson 1, Kurt Brorson 1, Scott Lute 1, David Roush 2, Joseph Hughes 3, Mike Burnham 3, 1 US Food and Drug Administration, Silver Spring, MD, USA; 2 Merck, Kenilworth, NJ, USA; 3 WuXi Apptec, Philadelphia, PA, USA 11:30 AM (L-310) Positive and Negative Mode Multi-column Processes for Enveloped Viruslike Particles Purification. Ricardo Silva 1, Alex Xenopoulos 2, Paula Alves 3, Manuel Carrondo 4, Cristina Peixoto 3, 1 ibet, Oeiras, PORTUGAL; 2 EMD Millipore, Bedford, MA, USA; 3 ibet/itqb, Oeiras, PORTUGAL; 4 ibet/fct-unl, Oeiras, PORTUGAL 11:50 AM (L-311) Purification of Cell Culture-based Influenza Virus Particles using Membrane Filters with the Aid of Non-ionic Polymers. Pavel Marichal-Gallardo 1, Michael Martin Pieler 1, Michael Wolff 2, Udo Reichl 2, 1 Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, GERMANY; 2 Max Planck Institute for Dynamics of Complex Technical Systems and Otto von Guericke University Magdeburg, Magdeburg, GERMANY 12:10 PM (L-312) The Assembly Phase of Virus-Like Particles Contributes Significantly to Separation and Purification. Mike Zhang, Frank Gillam, Virginia Polytechnic Institute and State University, Blacksburg, VA, USA 12:30-1:40 PM Lunch in Foyer PREP 2016 Final Program 20

29 Wednesday, July 20, Wednesday Session: Chromatography Fundamentals Session Chair: Jose Mota, IBET, Portugal Location: Commonwealth Hall A/B, 2nd floor 1:40 PM (L-313) Experimental and Theoretical Analysis of Chromatographic Processes in the Presence of Liquid-liquid Phase Separation. Franziska Ortner, Marco Mazzotti, ETH Zurich, Zurich, SWITZERLAND 2:00 PM (L-314) The Loadability and Retention Behavior of Zwitterionic Stationary Phases. Attila Felinger, University of Pecs, Pecs, HUNGARY 2:20 PM (L-315) Improving the Productivity of Capture Chromatography based on a Simplified Mechanistic Model. Noriko Yoshimoto, Sumiko Hasegawa, Shuichi Yamamoto, Yamaguchi University, Ube, JAPAN 2:40 PM (L-316) Robust Multi-objective Optimization of Chromatographic Rare Earth Element Separation. Hans-Kristian Knutson, Anders Holmqvist, Bernt Nilsson, Lund University, Lund, SWEDEN 3:00 PM (L-317) A Concurrent Approach for Isotherm Determination and Design of Simulated Moving Bed Process for Multicomponent Systems. Pranav Vengsarkar, Siwei Guo, Jason Bentley, Yoshiaki Kawajiri, Georgia Institute of Technology, Atlanta, GA, USA 14. Wednesday Session: Advances in CPC, CCC, and Chiral Separations Session Chair: Yoshiaki Kawajiri, Georgia Institute of Technology, Atlanta, GA, USA Location: Commonwealth Hall A/B, 2nd floor 3:20 PM (L-318) How Can Ion-pair CPC Make Joke of RPC at Preparative Levels? Laszlo Lorantfy 1, Dora Rutterschmidt 1, Zsolt Kovacs 2, Ying Hou Guan 3, Laszlo Frici Nemeth 1, 1 RotaChrom Technologiai, Dabas, HUNGARY; 2 ELTE, Budapest, HUNGARY; 3 BME, Budapest, HUNGARY 3:40 PM (L-319) Spiral Countercurrent Chromatography for Preparative Separations using Aqueous Two-phase Solvent Systems. Rodrigo Lazo-Portugal 1, Steingrimur Stephansson 2, Saeyoung Nate Ahn 2, Martha Knight 1, 1 CC Biotech LLC, Rockville, MD, USA; 2 Fuzbien Technology Inst., Rockville, MD, USA 4:00 PM (L-320) Cost-effectiveness Modeling to Understand the Contribution of Preparative Chiral Separations in Pharmaceutical R&D. Jeffrey Kiplinger, Paul Lefebvre, Emily Rouse, Keith Galyan, Averica Discovery, Marlborough, MA, USA 4:20 PM (L-321) Resolution of Mandelic Acid Enantiomers by Coupling Separation with Enantioselective Chromatography and Enzymatic Racemization. Katarzyna Wrzosek, Katja Bettenbrock, Andreas Seidel-Morgenstern, Max Planck Institute for Dynamics of Complex Technical Systems, Magdeburg, GERMANY 4:40 PM CLOSING REMARKS, Giorgio Carta, University of Virginia, Charlottesville, VA, USA 4:50 PM Farewell Reception PREP 2016 Final Program 21