Procedure: GFP cloning project Day 1. Bioinformatics and cloning workshop. Agar plate prep.

Save this PDF as:

Size: px
Start display at page:

Download "Procedure: GFP cloning project Day 1. Bioinformatics and cloning workshop. Agar plate prep."

Transcription

1 Procedure: GFP cloning project Day 1. Bioinformatics and cloning workshop. Agar plate prep. 1. Prepare nutrient agar (required in next few labs for bacterial work). a. To prepare the agar, weigh 3.85g LB-agar and pour into a 250ml Erlenmeyer flask. Luria Broth (LB) is a nutritionally rich medium suitable for bacterial growth; it contains tryptone, yeast and NaCL. Agar is a gelling agent that solidifies the mixture at room temperature. b. Add 110 ml of distilled water to a 250 ml Erlenmeyer flask. Cover the top with foil and label with your name and section using a small piece of tape on top of the foil. Use a permanent marker. c. Leave on center bench for the technician to autoclave (sterilize). Your LB-agar will sterilize at 121oC for 20 minutes. It will be ready to pour later during lab. Bioinformatics This portion of the lab will be performed in the computer lab. Bioinformatics is the application of computer technology to the storage and use of biological data, including protein and nucleic acid sequence and structural information. There are a plethora of websites containing databases, directories, catalogs and tools of interest to biochemists and molecular biologists. Below are listed but a few: Pedro s Biomolecular Research Tools (A Collection of WWW Links to Information and Services Useful to Molecular Biologists) CMS Molecular Biology Resources (A compendium of electronic and internet accessible tools and resources for Molecular Biology and Biochemistry) Protocol Online (Lab reference book) GenBank (The NIH genetic sequence database, an annotated collection of all publicly available DNA sequences), EMBL-EBI (databases of biological data including nucleic acid, protein sequences and macromolecular structures.) Protein Data Bank, PDB (Protein structures determined by X-ray and NMR) Swiss-protein (Protein sequences and analysis) AAindex (Properties of amino acids) BLAST (The Basic Local Alignment Search Tool finds regions of local similarity between sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance of matches. BLAST can be used to infer functional and evolutionary relationships between sequences as well as help identify members of gene families.) Chime (Chime is a browser plug-in that renders 2D and 3D molecules directly within a Web page. The molecules are "live", meaning they are not just static pictures, but chemical structures that scientists can rotate, reformat, and save in various file formats for use in modeling or database applications.)

2 For a more comprehensive list of websites please refer to: Biochemistry Laboratory, modern theory and techniques. Rodney Boyer. ISBN Benjamin Cummings press. Computational exercise (Cloning workshop): determining GFP gene sequence and PCR primer design. 1. Using the website EMBL-EBI, download and print the nucleotide sequence for Green florescent protein. Website: You will be searching for GFP, then click on Nucleotide sequences. Scroll down to results found in EMBL-Bank (Coding Sequence). 2. Determine what restriction enzyme sites are present within the GFP gene. Use the following website: Print your results. 3. Observe and print the X-ray crystallography structure of GFP using the Protein data bank. Use the following website and search for GFP: View the most recent citation. Click on view in Jmol, hold the left mouse key down and drag to rotate the molecule and view in different orientation. 4. Design primers for amplification of the GFP gene by PCR. Use to determine the reverse complement for the 3 primer. Use to determine the technical data on the primers you have designed and determine if they are appropriate for PCR.

3 Discussion: Generation of a GFP expressing plasmid (pglo) using the GFP PCR fragment and cloning it into an expression vector. pglo, 5.37Kb Return to the lab. Prepare the agar plates (you will use them for the transformation step in the next lab period): 1) The agar you prepared last lab period has been autoclaved (sterilized at 121oC for 20 minutes and pressure (~15psi)). While you are waiting for the agar to cool, label the plates. The agar plates should be marked with a permanent marker on the bottom close to the edge. Label each plate with your name and date. Label 1 plate LB, 1 plate LB/amp and 2 plate2 LB/amp/ara. 2) Sterile solutions of Arabinose (200mg/ml) and Ampicillim (10mg/ml) are provided. When adding these reagents to your LB-agar use sterile technique (your TA will demonstrate how). Sterile tips must be used, be careful not to contaminate the stocks or your LB-agar. Note: Excessive heat (>50 C) will destroy the ampicillin and the arabinose, but the nutrient agar solidifies at 27 C so one must carefully monitor the cooling of the agar and then pour the plates from start to finish without interruption. Excess bubbles can be removed after all the plates are poured by briefly flaming the surface of each plate with the flame of a Bunsen burner. After the plates are poured do not disturb them until the agar has solidified. Pour excess agar in the garbage, not the sink. Wipe any agar drips off of the sides of the plates.

4 Pour LB nutrient agar plates (LB, LB/amp, LB/amp/ara) YOUR TA WILL SHOW YOU HOW. 3) First, pour LB nutrient agar into the plate labeled LB. 4) Add 850ul of the ampicillin (10mg/mL) to the remaining LB nutrient agar. 5) Swirl briefly to mix. 6) Pour 25mL to the plate labeled as LB/amp using the technique utilized above. 7) Add 2mL of the arabinose to the remaining LB nutrient agar containing ampicillin. 8) Swirl briefly to mix and pour the plates labeled as LB/amp/ara using the technique utilized above. 9) LB broth. Place 2g LB broth into a 250ml Erlenmeyer flask, add 100mls ddh2o. Cover top with foil and label with your name. Leave on community bench for the technician to autoclave for use later on. 10) Place 4-5 eppendorf tubes in a 10mL beaker, cover with foil. Label and place on community bench. Interim Day: Place agar plates in a labeled plastic bag and store upside down in the cold room. They are stored upside down to prevent condensation dripping onto the agar. Day 2.Transformation. Collect your sterilized LB broth. We will provide the GFP plasmid for transformation. Each student should: 1. Label one closed sterile eppendorf tube +pgfp and another -pgfp. Label both tubes with your name. 2. Open the tubes and, using a sterile pipette, transfer 250 μl of transformation solution (50mM CaCl2) into each tube. 3. Place the tubes on ice. 3. Use a sterile loop to pick up a single colony of bacteria from your starter plate. Pick up the +pgfp tube and immerse the loop into the transformation solution at the bottom of the tube. Spin the loop between your index finger and thumb until the entire colony is dispersed in the transformation solution (with no floating chunks). Place the tube back on ice. Using a new sterile loop, repeat for the -pgfp tube. 4. Pipette 1uL of the GFP plasmid to the +GFP tube, mix gently with the pipette tip. Close the tube and return it to the ice. Also close the -pgfp tube. Do not add plasmid DNA to the - pgfp tube. 5. Incubate the tubes on ice for 10 minutes. 6. While the tubes are sitting on ice, label your four LB nutrient agar plates on the bottom (not the lid) as follows: Label one LB/amp plate: + pgfp Label the LB/amp/ara plate: + pgfp Label the other LB/amp/ara plate: - pgfp Label the LB plate: + pgfp 7. Heat shock. Using a foam rack as a holder, transfer both the (+) pgfp and (-) pgfp tubes into the water bath, set at 42 C, for exactly 50 seconds. Make sure to push the tubes all the way down in the rack so the bottoms of the tubes stick out and make contact with the warm water. When the 50 seconds are done, place both tubes back on ice. For the best transformation results, the transfer from the ice (0 C) to 42 C and then back to the ice must be rapid. Incubate tubes on ice for 2 minutes. 9. Remove the tubes from the ice and place on the bench top. Open a tube and, using a new sterile pipette, add 250 μl of LB nutrient broth to the tube and re-close it. Sterile technique requires that you pass the top of the LB broth

5 through a flame prior to pipetting. Repeat with a new sterile pipette for the other tubes. Incubate the tubes for 10 minutes at room temperature. 8. Tap the closed tubes with your finger to mix. Using a new sterile pipette for each tube, pipette 100 μl of the transformation and 100 μl control suspensions onto the appropriate nutrient agar plates. 11. Use a flamed sterilized loop for each plate. Spread the suspensions evenly around the surface of the LB nutrient agar by quickly skating the flat surface of a new sterile loop back and forth across the plate surface (your TA will demonstrate how). DO NOT PRESS TOO DEEP INTO THE AGAR. 9. Let solution absorb onto plates for ~15 minutes. 10. Stack up your plates and tape them together. Put your name and class period on the bottom of the stack and place the stack of plates upside down in the 37 C incubator until the next day. 11. Prepare 200mls of TE buffer. 12. Prepare 10mls each of column equilibration buffer and column wash buffer using the calculations from your pre-lab. Store in cold room for later use. Prep your culture tubes for interim day: 13. Obtain two 15ml sterile culture tubes. Mark with your name. 14. Using sterile technique, pipette 3mL LB broth into each tube. 15. Pipette the amount of ampicillin you calculated in your pre-lab into each tube. 16. Pipette the amount of L-arabinose you calculated in your pre-lab into each tube. 17. Store in cold room for use in the interim day. Interim day 1. Examine your LB/amp and LB/amp/ara plates from the transformation lab. First use normal room lighting, and then use an ultraviolet light in a darkened area of your laboratory. Note your observations. To prevent damage to your skin or eyes, avoid exposure to the UV light. Never look directly into the UV lamp. Wear safety glasses whenever possible. 2. Identify several green colonies that are not touching other colonies on the LB/amp/ara plate. Turn the plate over and circle several of these green colonies. On the other LB/amp plate identify and circle several white colonies that are also well isolated from other colonies on the plate. 3. Obtain the two 15 ml culture tubes that you prepared in the previous lab. Using a sterile inoculation loop, lightly touch the "loop" end to a circled single green colony and scoop up the cells without grabbing big chunks of agar. Immerse the loop in one culture tube. Spin the loop between your index finger and thumb to disperse the entire colony. Repeat for the other culture tube. Streak an Amp/Ara plate with the loop and place in incubator. 4. Cap your tubes and place them in the shaking water bath. Let the tubes incubate for 24 hours at 32 C. Day 3. Plasmid Preparation and analysis. 1. Retrieve culture tubes from the shaking water bath. 2. Observe florescence with UV lamp. 3. Place tubes in the clinical centrifuge (be sure to balance the centrifuge by placing the two tubes in opposite positions) and spin at top speed for 15 minutes. 4. Carefully pour off liquid.

6 5. Resuspend each pellet in 1 ml ddh2o and transfer to two 1.5ml eppendorf tubes. Spin for 2 minutes at 10,000xg to pellet cells. 6. Pipette off supernatant and discard. 7. Resuspend each pellet in 200ul resuspension solution. 8. Add 200ul cell lysis solution, mix well. 9. Add 200ul neutralization solution, mix well. 10. Centrifuge at 10,000xg for 5 minutes. 11. Assemble two minicolumns each with a 3ml syringe barrel. Shake resin well and add 1ml resin. 12. Transfer the supernatant from step 12. to the two columns. 13. Insert syringe plunger and press to eject liquid (do not save). 14. Remove column from syringe. 15. Remove plunger. 16. Re-attach column to syringe. 17. Add 2mls column wash solution to each syringe. 18. Insert syringe plunger and press to eject liquid (do not save). 19. Remove syringe barrel and place each column in a 1.5ml eppendorf tube. 20. Centrifuge at 10,000xg for 2 minutes (be sure to balance the centrifuge with another students sample). 21. Transfer each minicolumn to a new eppendorf tube. 22. Add 30ul sterile water to each and wait 1 minute. 23. Centrifuge at 10,000xg for 20 seconds. Restriction enzyme analysis. 1. Using the pglo plasmid map notes from your pre-lab set up a suitable diagnostic enzyme digest for your plasmid prep. Incubate at 37oC for 1 hour and then at 65oC for 15 minutes. a. 15uL plasmid b. 2uL buffer (see chart for appropriate buffer) c. 1uL BSA (from diluted stock) d. 1uL sterile water e. 1uL restriction enzyme 2. Pour a 1% DNA agarose gel. 3. Load gel with digest samples and ladder. Run at 100V for 40 minutes. 4. Pour 50mL 1x Sybr gold solution into staining tray. Add gel. 5. Cover with foil and agitate for 10 minutes. 6. View and photograph gel. Prepare culture tubes and set up cultures for protein purification. 1. Obtain two 15ml sterile culture tubes. Mark with your name. 2. Using sterile technique, pipette 3mls LB broth into each tube. 3. Pipette the amount of ampicillin you calculated in your pre-lab into each tube. 4. Pipette the amount of L-arabinose you calculated in your pre-lab into each tube. 5. Inoculate each tube with a GFP expressing colony.

Bacterial Transformation and Protein Purification

Bacterial Transformation and Protein Purification Bacterial Transformation and Protein Purification Group 4 Natalie Beale Gregory A. Pate Justin Rousseau Dohee Won Introduction The purpose of this experiment is to perform a genetic transformation and

More information

Student Manual. pglo Transformation

Student Manual. pglo Transformation Student Manual pglo Transformation Lesson 1 Introduction to Transformation In this lab you will perform a procedure known as genetic transformation. Remember that a gene is a piece of DNA which provides

More information

Transforming E. Coli with pglo Plasmids

Transforming E. Coli with pglo Plasmids Name: Transforming E. Coli with pglo Plasmids AP Biology Transformation Background: Transformation is a process of transferring genetic information from one organism to another. In bacteria, a small circular

More information

Bacterial genetic exchange : Bacterial Transformation

Bacterial genetic exchange : Bacterial Transformation Experiment 11 Laboratory to Biology III: Diversity of Microorganisms 1 Experiment 11 Bacterial genetic exchange : Bacterial Transformation Advisor Munti Yuhana myuhana@botinst.unizh.ch Textbook Chapters

More information

Student Manual. pglo Transformation

Student Manual. pglo Transformation Student Manual pglo Transformation STUDENT MANUAL LESSON 1 Lesson 1 Introduction to Transformation In this lab you will perform a procedure known as genetic transformation. Remember that a gene is a piece

More information

Amgen Protocol: Introduction and a few comments:

Amgen Protocol: Introduction and a few comments: Amgen Protocol: Introduction and a few comments: The following is a shortened version of the Amgen Lab. This series of labs involves the creation of a recombinant plasmid, subsequent transformation of

More information

Lab 1 Flow Chart : Learning basic laboratory skills

Lab 1 Flow Chart : Learning basic laboratory skills Lab Flow Chart : Learning basic laboratory skills RD Red dye solution S Dye S2 Dye 2 S3 Dye 3 H 2 O Water X TAE X Lab.: Basic pipetting and serial dilution 2 Plunger button Tip ejector Display window Barrel

More information

Purification of mfp. from an Overnight Culture. Laboratory 17

Purification of mfp. from an Overnight Culture. Laboratory 17 Purification of mfp from an Overnight Culture When scientists at a therapeutics company, like Amgen, have successfully identified a promising therapeutic protein, two objectives would be to locate and

More information

Biology Lab Activity 4-5 DNA Transformation

Biology Lab Activity 4-5 DNA Transformation Biology Lab Activity 4-5 DNA Transformation Scientists can insert genes into bacteria. The genes inserted in the Indo-Blu process (this lab) are on a circular piece of DNA called a plasmid. (The plasmid

More information

EXPERIMENT GENOMIC DNA ANALYSIS

EXPERIMENT GENOMIC DNA ANALYSIS EXPERIMENT GENOMIC DNA ANALYSIS Population diversity Studies We have 5 species of planarians (3 purchased from Carolina Biologicals, 2 obtained from the Levin lab) andmight have additional species found

More information

Human DNA Alu Amplification by Polymerase Chain Reaction (PCR)* Laboratory Procedure

Human DNA Alu Amplification by Polymerase Chain Reaction (PCR)* Laboratory Procedure Human DNA Alu Amplification by Polymerase Chain Reaction (PCR)* Laboratory Procedure *Polymerase Chain Reaction is covered by patents owned by Hoffmann-La Roche, Inc. This experiment was adapted from Laboratory

More information

In order to do transformation, the gene to be transferred is placed into a plasmid. This is done with the help of restriction enzymes, 7

In order to do transformation, the gene to be transferred is placed into a plasmid. This is done with the help of restriction enzymes, 7 Fluorescent Protein Transformation Student Background Genetic transformation occurs when a cell takes up (i.e. takes inside) and expresses a new piece of genetic material DNA. Genetic transformation literally

More information

Purification and Characterization of a DNA Plasmid Part A CHEM 4581: Biochemistry Laboratory I Version: January 18, 2008

Purification and Characterization of a DNA Plasmid Part A CHEM 4581: Biochemistry Laboratory I Version: January 18, 2008 Purification and Characterization of a DNA Plasmid Part A CHEM 4581: Biochemistry Laboratory I Version: January 18, 2008 INTRODUCTION DNA Plasmids. A plasmid is a small double-stranded, circular DNA molecule

More information

GeNei TM Transformation Teaching Kit Manual

GeNei TM Transformation Teaching Kit Manual Teaching Kit Manual Cat No. New Cat No. KT07 107385 KT07A 106220 Revision No.: 00060505 CONTENTS Page No. Objective 3 Principle 3 Kit Description 6 Materials Provided 7 Procedure 9 Observation & Interpretation

More information

QIAfilter Plasmid Midi Kit (Cat #: 12243)

QIAfilter Plasmid Midi Kit (Cat #: 12243) QIAfilter Plasmid Midi Kit (Cat #: 12243) Things to do before starting Add the provided RNase A solution to Buffer P1 before use. Use one vial of RNase A (centrifuge briefly before use) per bottle of Buffer

More information

Genetic Engineering: Transforming Bacteria

Genetic Engineering: Transforming Bacteria Genetic Engineering: Transforming Bacteria Introduction Activity Introduction In this laboratory experiment, students will transform the phenotype of bacteria through the use of genetic engineering. A

More information

VDL100.2 CLONING TRANSGENE INTO padenox

VDL100.2 CLONING TRANSGENE INTO padenox 1. Purpose 1.1. The purpose of this protocol is to transfer a transgene from the pshuttlex plasmid to padenox. 1.2. The starting material is 10 μg plasmid DNA. 1.3. This procedure is routinely performed

More information

FosmidMAX DNA Purification Kit

FosmidMAX DNA Purification Kit Cat. No. FMAX046 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com Lit. # 204 10/2012 1 EPILIT204 Rev. A

More information

Plasmid Midiprep Plus Purification Kit. Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only

Plasmid Midiprep Plus Purification Kit. Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only Plasmid Midiprep Plus Purification Kit Cat. # : DP01MD-P10/ DP01MD-P50 Size : 10/50 Reactions Store at RT For research use only 1 Description: The Plasmid Midiprep Plus Purification Kit provides simple

More information

BIOLOGY 163 LABORATORY. RESTRICTION MAPPING OF PLASMID DNA (Revised Fall 2017)

BIOLOGY 163 LABORATORY. RESTRICTION MAPPING OF PLASMID DNA (Revised Fall 2017) BIOLOGY 163 LABORATORY RESTRICTION MAPPING OF PLASMID DNA (Revised Fall 2017) Physical mapping of genomes is an important part of modern molecular genetics. As it's name implies, physical mapping seeks

More information

UltraClean Midi Plasmid Prep Kit

UltraClean Midi Plasmid Prep Kit UltraClean Midi Plasmid Prep Kit Catalog No. Quantity 12700-20 20 Preps Instruction Manual Please recycle Version: 05232014 1 Table of Contents Introduction... 3 Protocol Overview... 3 Flow Chart... 4

More information

Molecular Techniques Third-year Biology

Molecular Techniques Third-year Biology PLANNING Genetics Lab practices Molecular Techniques. Genetics Lab practices protocol. 2015-16 PCR-DIRECTED MUTAGENESIS, MOLECULAR CLONING AND RESTRICTION ANALYSIS Sessions 1 & 2 (2x3 hours): PCR-directed

More information

Average Yields* Yeast DNA Yeast RNA Time to Complete 10 Purifications * Yield will vary depending on the type of sample processed

Average Yields* Yeast DNA Yeast RNA Time to Complete 10 Purifications * Yield will vary depending on the type of sample processed 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Fungi/Yeast RNA/DNA Purification Kit Product # 35800 Product Insert

More information

Cat # Box1 Box2. DH5a Competent E. coli cells CCK-20 (20 rxns) 40 µl 40 µl 50 µl x 20 tubes. Choo-Choo Cloning TM Enzyme Mix

Cat # Box1 Box2. DH5a Competent E. coli cells CCK-20 (20 rxns) 40 µl 40 µl 50 µl x 20 tubes. Choo-Choo Cloning TM Enzyme Mix Molecular Cloning Laboratories User Manual Version 3.3 Product name: Choo-Choo Cloning Kits Cat #: CCK-10, CCK-20, CCK-096, CCK-384 Description: Choo-Choo Cloning is a highly efficient directional PCR

More information

Site-directed mutagenesis of proteins

Site-directed mutagenesis of proteins IFM/Kemi Linköpings Universitet August 2013/LGM Labmanual Site-directed mutagenesis of proteins Figur 1: Flow-chart of the site-directed mutagenesis lab exercise 2 Site-specific mutagenesis Introduction

More information

MOLECULAR GENETICS: TRANSFORMATION AND CLONING adapted by Dr. D. L. Vogelien

MOLECULAR GENETICS: TRANSFORMATION AND CLONING adapted by Dr. D. L. Vogelien Introduction MOLECULAR GENETICS: TRANSFORMATION AND CLONING adapted by Dr. D. L. Vogelien The field of molecular genetics has resulted in a number of practical applications that have been of tremendous

More information

BACMAX DNA Purification Kit

BACMAX DNA Purification Kit Cat. No. BMAX044 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com Lit. # 212 10/2012 1 EPILIT212 Rev. A

More information

HiPer Random Amplification of Polymorphic DNA (RAPD) Teaching Kit

HiPer Random Amplification of Polymorphic DNA (RAPD) Teaching Kit HiPer Random Amplification of Polymorphic DNA (RAPD) Teaching Kit Product Code: HTBM031 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 3.5 hours Agarose Gel Electrophoresis:

More information

LAB 6: Agarose Gel Electrophoresis of Restriction Digested Plasmid DNA

LAB 6: Agarose Gel Electrophoresis of Restriction Digested Plasmid DNA LAB 6: Agarose Gel Electrophoresis of Restriction Digested Plasmid DNA I. Objectives The purpose of today s lab is to learn how to set up and run an agarose gel, separate DNA fragments on the gel, and

More information

ASEPTIC TRANSFER & PURE CULTURE TECHNIQUES

ASEPTIC TRANSFER & PURE CULTURE TECHNIQUES ASEPTIC TRANSFER & PURE CULTURE TECHNIQUES GENERAL GUIDELINES & REMINDERS: SAFETY: NO EATING OR DRINKING IN THE LAB! Wash your hands with soap both BEFORE and AFTER lab, and, in addition, when you have

More information

PowerSoil DNA Isolation Kit

PowerSoil DNA Isolation Kit PowerSoil DNA Isolation Kit Catalog No. Quantity 12888-50 50 Preps 12888-100 100 Preps Instruction Manual Introduction The PowerSoil DNA Isolation Kit* is comprised of a novel and proprietary method for

More information

Alkaline Lysis Large Scale Plasmid Preparation

Alkaline Lysis Large Scale Plasmid Preparation Alkaline Lysis Large Scale Plasmid Preparation 1. Set up 10 ml overnight culture. 2. Add overnight to 500 mls of sterile LB with appropriate selective agent (e.g amp, tet...) 3. Incubate at 37 C with shaking

More information

Aseptic Techniques. A. Objectives. B. Before coming to lab

Aseptic Techniques. A. Objectives. B. Before coming to lab Aseptic Techniques A. Objectives Become familiar with 1. The ubiquity of microorganisms (see Note 1) 2. Aseptic techniques (see Note 2) 3. Standard methods for growing/observing microorganisms (see Note

More information

HiPer Yeast Genomic DNA Extraction Teaching Kit

HiPer Yeast Genomic DNA Extraction Teaching Kit HiPer Yeast Genomic DNA Extraction Teaching Kit Product Code: HTBM013 Number of experiments that can be performed: 10 Duration of Experiment: 3 days Day 1: Revival of Host Day 2: Inoculation of culture

More information

HiPer Transformation Teaching Kit

HiPer Transformation Teaching Kit HiPer Transformation Teaching Kit Product Code: HTBM017 Number of experiments that can be performed: 10 Duration of Experiment: 4 days Day 1- Preparation of media and revival of E. coli Host Day 2- Inoculation

More information

EZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK

EZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK EZ-0 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK (Bacteria, Plant, Animal, Blood) Version 5.0 Rev 03/25/205 Table of Contents Introduction 2 Limitations of Use 2 Features 2 Applications 2 Storage 2

More information

Transformation of DNA in competent E. coil

Transformation of DNA in competent E. coil Transformation of DNA in competent E. coil Reagents: SOC medium (1L) (a) 20g tryptone, 5g yeast extract, 0.5g NaCl in 950ml dh 2 O. (b) 250mM KCl: 1.86 KCl in 100ml dh 2 O. Add 10ml of solution (b) to

More information

HiPer Plasmid DNA Cloning Teaching Kit

HiPer Plasmid DNA Cloning Teaching Kit HiPer Plasmid DNA Cloning Teaching Kit Product Code: HTBM022 Number of experiments that can be performed: 5 Duration of Experiment: 4 days Day 1- Preparation of media and revival of E. coli Host Day2-

More information

HiPer RT-PCR Teaching Kit

HiPer RT-PCR Teaching Kit HiPer RT-PCR Teaching Kit Product Code: HTBM024 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 4 hours Agarose Gel Electrophoresis: 45 minutes Storage Instructions: The

More information

ONTARIO SCIENCE CENTRE. Teacher Guide. Way to Glow Program

ONTARIO SCIENCE CENTRE. Teacher Guide. Way to Glow Program ONTARIO SCIENCE CENTRE Teacher Guide Way to Glow Program Table of Contents Bacterial transformation background information 3 Experimental procedure 5 Expected results 7 Post-program activity sheet 8 Post-program

More information

PROTOCOL 1: EXTRACTION OF DNA FROM BACTERIA

PROTOCOL 1: EXTRACTION OF DNA FROM BACTERIA PROTOCOL 1: EXTRACTION OF DNA FROM BACTERIA The basic standard procedures for isolation of bacterial DNA are based on lysozyme digestion of the cell wall, detergent lysis, disruption of protein-nucleic

More information

PURE CULTURE TECHNIQUES

PURE CULTURE TECHNIQUES PURE CULTURE TECHNIQUES Most specimens (from animal tissue, plant tissue, or environmental samples) will be mixed, with a variety of bacteria (or other microorganisms). A single gram of feces, for example,

More information

E.Z.N.A. Bacterial RNA Kit. R preps R preps

E.Z.N.A. Bacterial RNA Kit. R preps R preps E.Z.N.A. Bacterial RNA Kit R6950-00 5 preps R6950-01 50 preps July 2017 E.Z.N.A. Bacterial RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Before Beginning...4

More information

HiPer Gel Extraction Teaching Kit (Column Based)

HiPer Gel Extraction Teaching Kit (Column Based) HiPer Gel Extraction Teaching Kit (Column Based) Product Code: HTBM010 Number of experiments that can be performed: 10 Duration of Experiment Agarose Gel Electrophoresis: 1 hour Protocol: 1 hour Agarose

More information

Purification of DNA Oligonucleotides

Purification of DNA Oligonucleotides Purification of DNA Oligonucleotides Polyacrylamide gel: In a beaker, add 30 ml 30% acrylamide solution (To make this solution, add 29 g acrylamide to 1 g bis-acrylamide, bring to 100 ml with dh 2 O) 31.5

More information

E.Z.N.A. Stool DNA Kit. D preps D preps D preps

E.Z.N.A. Stool DNA Kit. D preps D preps D preps E.Z.N.A. Stool DNA Kit D4015-00 5 preps D4015-01 50 preps D4015-02 200 preps April 2013 E.Z.N.A. Stool DNA Kit Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents/Storage

More information

TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE

TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE TRANSFER OF BACTERIA USING ASEPTIC TECHNIQUE GENERAL GUIDELINES: Safety Wear a lab coat and have your goggles on! ALWAYS disinfect the tables BEFORE and AFTER lab. Wash your hands with soap both BEFORE

More information

Product # Kit Specification. Kit Specifications Maximum Column Binding Capacity 50 µg Maximum Column Loading Volume 650 µl

Product # Kit Specification. Kit Specifications Maximum Column Binding Capacity 50 µg Maximum Column Loading Volume 650 µl 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Fungi/Yeast Genomic DNA Isolation Kit Product # 27300 Product

More information

Plasmid Subcloning using low melt ligation

Plasmid Subcloning using low melt ligation Design Considerations Plasmid Subcloning using low melt ligation General 1) We much prefer directional cloning (since it usually works better and takes less time) and we have found that with the help of

More information

Specifications. Kit Specifications. Alcohol Precipitation: Up to 100 ml Column Purification: Up to 5 ml Column Binding Capacity 25 µg

Specifications. Kit Specifications. Alcohol Precipitation: Up to 100 ml Column Purification: Up to 5 ml Column Binding Capacity 25 µg 3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com BAC DNA MiniPrep Kit Product # 18050 Product Insert The BAC DNA

More information

Section IX: Special Applications in Agarose Gels

Section IX: Special Applications in Agarose Gels Section IX: In This Section Amplification of Plasmid cdna Libraries with SeaPrep Agarose 150 Preparing Agarose for use in Cell Culture Applications 152 References 154 149 Section IX: Amplification of Plasmid

More information

EZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK

EZ-10 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK EZ-0 SPIN COLUMN GENOMIC DNA MINIPREPS KIT HANDBOOK (Bacteria, Plant, Animal, Blood) Version 8 Rev 05/0/03 EZ-0 Genomic DNA Kit Handbook Table of Contents Introduction Limitations of Use Features Applications

More information

E.Z.N.A. Tissue RNA Kit. R preps R preps

E.Z.N.A. Tissue RNA Kit. R preps R preps E.Z.N.A. Tissue RNA Kit R6688-00 5 preps R6688-01 50 preps May 2015 E.Z.N.A. Tissue RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Important Notes...4 Homogenization

More information

Dolan DNA Learning Center Glowing Genes

Dolan DNA Learning Center Glowing Genes Dolan DNA Learning Center Glowing Genes Background History A chromosome is a continuous DNA molecule that can be thousands or millions of base pairs long. The vast length of chromosomes posed a problem

More information

EQUIPMENTS & MATERIALS COMMONLY USED IN A LABORATORY

EQUIPMENTS & MATERIALS COMMONLY USED IN A LABORATORY EQUIPMENTS & MATERIALS COMMONLY USED IN A LABORATORY a) Autoclave: An autoclave is a device used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 C for around

More information

ReadyAmp Genomic DNA Purification System INSTRUCTIONS FOR USE OF PRODUCTS A7710.

ReadyAmp Genomic DNA Purification System INSTRUCTIONS FOR USE OF PRODUCTS A7710. Technical Bulletin ReadyAmp Genomic DNA Purification System INSTRUCTIONS FOR USE OF PRODUCTS A7710. PRINTED IN USA. Revised 4/11 ReadyAmp Genomic DNA Purification System All technical literature is available

More information

Protocol for DNA extraction from FFPE Samples

Protocol for DNA extraction from FFPE Samples 25, ave Georges Lemaître - B-6041 Gosselies (Belgique) Tel : +32 (0)71 37 85 27 - Fax : +32 (0)71 34 78 79 Protocol for DNA extraction from FFPE Samples Step 1. Paraffin Removal 1 Equilibrate a heat block

More information

Note: for laboratory research use only. RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Signalway Biotechnology

Note: for laboratory research use only. RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Signalway Biotechnology Note: for laboratory research use only RNA High-purity Total RNA Rapid Extraction Kit (Spin-column) Cat. #: RP1202 (50preps) Signalway Biotechnology I. Kit Content, Storage Condition and Stability Content

More information

Guide-it Indel Identification Kit User Manual

Guide-it Indel Identification Kit User Manual Clontech Laboratories, Inc. Guide-it Indel Identification Kit User Manual Cat. No. 631444 (120114) Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Avenue, Mountain View, CA 94043, USA

More information

qpcr Kit, DNA-free Product components 100 rxn 250 rxn Product description

qpcr Kit, DNA-free Product components 100 rxn 250 rxn Product description qpcr Kit, DNA-free For the PCR detection and identification of bacterial and fungal DNA using custom primers Product code A8514 Product components 100 rxn 250 rxn A 2.5x mastermix (3 mm MgCl 2 final concentration)

More information

E.Z.N.A. Stool DNA Kit. D preps D preps D preps

E.Z.N.A. Stool DNA Kit. D preps D preps D preps E.Z.N.A. Stool DNA Kit D4015-00 5 preps D4015-01 50 preps D4015-02 200 preps July 2017 E.Z.N.A. Stool DNA Kit Table of Contents Introduction and Overview...2 Illustrated Protocol...3 Kit Contents/Storage

More information

Lambda DNA Purification Kit

Lambda DNA Purification Kit Lambda DNA Purification Kit INSTRUCTION MANUAL Catalog #200391 and #200392 Revision A For In Vitro Use Only 200391-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this

More information

PowerMax Soil DNA Isolation Kit

PowerMax Soil DNA Isolation Kit PowerMax Soil DNA Isolation Kit Catalog No. Quantity 12988-10 10 Preps Instruction Manual Inhibitor Removal Technology (IRT) is a registered trademark of MO BIO Laboratories, Inc. and is covered by the

More information

RFLP ANALYSIS OF DNA LABORATORY

RFLP ANALYSIS OF DNA LABORATORY RFLP ANALYSIS OF DNA LABORATORY BIG PICTURE You will be working with an essential research method widely used in genetics, conservation biology, and forensics. The lab is divided into three sections. Part

More information

CopyCutter EPI400 Electrocompetent E. coli. CopyCutter EPI400 Chemically Competent E. coli

CopyCutter EPI400 Electrocompetent E. coli. CopyCutter EPI400 Chemically Competent E. coli Cat. Nos. C400EL10, C400CH10, and CIS40025 CopyCutter EPI400 Electrocompetent and Chemically Competent E. coli* cells were developed to significantly lower the copy number of a wide variety of common vectors

More information

ProductInformation. Genomic DNA Isolation Kit. Product No. GDI-3 Technical Bulletin No. MB-275 May 2000 TECHNICAL BULLETIN

ProductInformation. Genomic DNA Isolation Kit. Product No. GDI-3 Technical Bulletin No. MB-275 May 2000 TECHNICAL BULLETIN Genomic DNA Isolation Kit Product No. GDI-3 Technical Bulletin No. MB-275 May 2000 TECHNICAL BULLETIN ProductInformation Product Description Sigma s Genomic DNA Isolation Kit isolates genomic DNA from

More information

DNA RESTRICTION ANALYSIS

DNA RESTRICTION ANALYSIS DNA RESTRICTION ANALYSIS In this experiment, DNA from the bacteriophage Lambda (48,502 base pairs in length) is cut with a variety of restriction enzymes and the resulting fragments are separated using

More information

E.Z.N.A. Plasmid DNA Mini Kit I

E.Z.N.A. Plasmid DNA Mini Kit I E.Z.N.A. Plasmid DNA Mini Kit I D6942-00 5 preps Q - Spin D6942-01 50 preps Q - Spin D6942-02 200 preps Q - Spin D6943-00 5 preps V - Spin D6943-01 50 preps V - Spin D6943-02 200 preps V - Spin E.Z.N.A.

More information

TruSeq ChIP Sample Preparation

TruSeq ChIP Sample Preparation FOR RESEARCH USE ONLY Date: Illumina Kit Description: NOTE Unless familiar with the protocol in the latest version of the TruSeq ChIP Sample Preparation Guide (part # 15023092), new or less experienced

More information

Experiment 3: Microbial Techniques

Experiment 3: Microbial Techniques Experiment 3: Microbial Techniques Objectives: By the end of this lab, you will be able to: 1. Understand and practice aseptic techniques in handling microorganisms. 2. Learn simple media preparation procedures

More information

For simultaneous purification of genomic DNA and total RNA from the same animal cells or tissues

For simultaneous purification of genomic DNA and total RNA from the same animal cells or tissues 1. TIANamp DNA/RNA Isolation Kit For simultaneous purification of genomic DNA and total RNA from the same animal cells or tissues www.tiangen.com/en RP090603 TIANamp DNA/RNA Isolation Kit Kit Contents

More information

E.Z.N.A. Water DNA Kit. D preps D preps D preps

E.Z.N.A. Water DNA Kit. D preps D preps D preps E.Z.N.A. Water DNA Kit D5525-00 5 preps D5525-01 50 preps D5525-02 200 preps April 2017 E.Z.N.A. Water DNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Catalog No. SA-40002: 50 preparation SA-40001: 100 preparation

Catalog No. SA-40002: 50 preparation SA-40001: 100 preparation BDtract TM Genomic DNA Isolation Kit Instruction Manual Catalog No. SA-40002: 50 preparation SA-40001: 100 preparation Maxim Biotech, Inc. 780 Dubuque Avenue, So. San Francisco, CA 94080, U.S.A. Tel: (800)

More information

For in vitro Veterinary Diagnostics only. DNA Extraction and PCR Detection Kit for Pasteurella multocida.

For in vitro Veterinary Diagnostics only. DNA Extraction and PCR Detection Kit for Pasteurella multocida. For in vitro Veterinary Diagnostics only. DNA Extraction and PCR Detection Kit for Pasteurella multocida www.kylt.eu DIRECTION FOR USE Art. No. 31058 / 31059 Kylt Pasteurella multocida DNA Extraction and

More information

E.Z.N.A. Yeast Plasmid Mini Kit. D preps D preps

E.Z.N.A. Yeast Plasmid Mini Kit. D preps D preps E.Z.N.A. Yeast Plasmid Mini Kit D3376-00 5 preps D3376-01 50 preps November 2015 E.Z.N.A. Yeast Plasmid Mini Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

GeNei TM Gel Extraction Teaching Kit Manual

GeNei TM Gel Extraction Teaching Kit Manual Teaching Kit Manual Cat No. New Cat No. KT43 106279 KT43A 106300 KT43B 106301 Revision No.: 00280507 CONTENTS Page No. Objective 3 Principle 3 Kit Description 5 Materials Provided 7 Procedure 8 Observation

More information

Polymerase Chain Reaction (PCR)

Polymerase Chain Reaction (PCR) Laboratory for Environmental Pathogens Research Department of Environmental Sciences University of Toledo Polymerase Chain Reaction (PCR) Background information The polymerase chain reaction (PCR) is an

More information

Table 1.1. Reagents Preparation Reagent Temp Out of Module* Treatment Store before using in Master Mix

Table 1.1. Reagents Preparation Reagent Temp Out of Module* Treatment Store before using in Master Mix Quick Reference Card Axiom Manual Target Prep Protocol Stage 1: DNA Amplification Intro to Manual Target Preparation Running the Axiom Assay requires the following sets of steps: 1. Genomic DNA Prep, described

More information

minipcr TM Genes in Space Food Safety Lab: Mars Colony at Risk!

minipcr TM Genes in Space Food Safety Lab: Mars Colony at Risk! minipcr TM Genes in Space Food Safety Lab: Mars Colony at Risk! An E. coli outbreak affects astronaut food aboard the International Space Station. DNA samples from two food racks are analyzed to determine

More information

Aurum Plasmid Mini Kit. Instruction Manual. Bio-Rad Laboratories, Inc Alfred Nobel Dr. Hercules, CA USA (510)

Aurum Plasmid Mini Kit. Instruction Manual. Bio-Rad Laboratories, Inc Alfred Nobel Dr. Hercules, CA USA (510) Bio-Rad Laboratories, Inc. 2000 Alfred Nobel Dr. Hercules, CA 94547 USA (510) 741-1000 1-800-424-6723 Aurum Plasmid Mini Kit Instruction Manual For technical service, call your local Bio-Rad office, or

More information

GeneMATRIX Gram Plus & Yeast Genomic DNA Purification Kit

GeneMATRIX Gram Plus & Yeast Genomic DNA Purification Kit GeneMATRIX Gram Plus & Yeast Genomic DNA Purification Kit Universal kit for isolation of total DNA from gram positive bacteria and yeast. The kit contains glass beads for mechanical cell disruption. Cat.

More information

Introduction. Principle

Introduction. Principle Contents Introduction............................................................ 2 Principle.............................................................. 2 Storage and Stability.....................................................

More information

Lambda (λ) DNA Restriction Digest and Electrophoresis Lab

Lambda (λ) DNA Restriction Digest and Electrophoresis Lab Lambda (λ) DNA Restriction Digest and Electrophoresis Lab Procedure DAY ONE: restriction digestion Today we will be exposing the lambda DNA to restriction enzymes. For background knowledge, make sure you

More information

Pinpoint Slide DNA Isolation System Catalog No. D3001

Pinpoint Slide DNA Isolation System Catalog No. D3001 INSTRUCTIONS Pinpoint Slide DNA Isolation System Catalog No. D3001 Highlights Easily isolates genomic DNA in any targeted microscopic tissue area on a slide. The simple procedure combines Pinpoint tissue

More information

Gateway Cloning Protocol (Clough Lab Edition) This document is a modification of the Gateway cloning protocol developed by Manju in Chris Taylor's lab

Gateway Cloning Protocol (Clough Lab Edition) This document is a modification of the Gateway cloning protocol developed by Manju in Chris Taylor's lab Gateway Cloning Protocol (Clough Lab Edition) This document is a modification of the Gateway cloning protocol developed by Manju in Chris Taylor's lab With the Gateway cloning system, a PCR fragment is

More information

Biotechnology Explorer

Biotechnology Explorer arac ori pglo bla GFP Biotechnology Explorer pglo Bacterial Transformation Kit Catalog #166-0003EDU explorer.bio-rad.com See individual components for storage temperature. Duplication of any part of this

More information

SAMPLE LITERATURE. Please refer to included weblink for correct version. Colony PCR. Edvo-Kit #323. GFP Transformation Extension:

SAMPLE LITERATURE. Please refer to included weblink for correct version. Colony PCR. Edvo-Kit #323. GFP Transformation Extension: NOTE: This experiment is designed to work with EDVOTEK Kits 222, 223, or 303. Please refer to page 19 for specifics. Edvo-Kit #323 GFP Transformation Extension: Colony PCR Experiment Objective: In this

More information

BioBrick Formation Step-by-Step Overview: (protocol compiled by Bethany Bruno, Liz Kelly, & Elyse McMillen, last updated 9/24/13)

BioBrick Formation Step-by-Step Overview: (protocol compiled by Bethany Bruno, Liz Kelly, & Elyse McMillen, last updated 9/24/13) BioBrick Formation Step-by-Step Overview: (protocol compiled by Bethany Bruno, Liz Kelly, & Elyse McMillen, last updated 9/24/13) As a multistep, complex process, each aspect of biobrick formation takes

More information

The Production of a Recombinant Biotechnology Product. Chapter 8

The Production of a Recombinant Biotechnology Product. Chapter 8 The Production of a Recombinant Biotechnology Product Chapter 8 Objectives Give a basic overview of genetic engineering. Describe the processes involved in isolating a piece DNA of interest Mass producing

More information

Geneaid DNA Isolation Kit (Yeast)

Geneaid DNA Isolation Kit (Yeast) Geneaid DNA Isolation Kit (Yeast) GEY100, GEY300 Advantages Sample: up to 2 10 8 yeast and other fungus species Yield: high yield, high quality DNA (A260/A280 = 1.8-2.0) Format: scalable DNA precipitation

More information

DNA Extraction Kit INSTRUCTION MANUAL. Catalog # Revision B. For In Vitro Use Only

DNA Extraction Kit INSTRUCTION MANUAL. Catalog # Revision B. For In Vitro Use Only DNA Extraction Kit INSTRUCTION MANUAL Catalog #200600 Revision B For In Vitro Use Only 200600-12 LIMITED PRODUCT WARRANTY This warranty limits our liability to replacement of this product. No other warranties

More information

PowerMag Soil DNA Isolation Kit (Optimized for KingFisher )

PowerMag Soil DNA Isolation Kit (Optimized for KingFisher ) PowerMag Soil DNA Isolation Kit (Optimized for KingFisher ) Catalog No. Quantity Total Purifications 27000-4-KF 4 x 96 Preps (Flex) 384 or 32 x 12 Preps (Duo) Instruction Manual Please recycle Version:

More information

Lesson 3 Gel Electrophoresis of Amplified PCR Samples and Staining of Agarose Gels

Lesson 3 Gel Electrophoresis of Amplified PCR Samples and Staining of Agarose Gels Lesson 3 Gel Electrophoresis of Amplified PCR Samples and Staining of Agarose Gels What Are You Looking At? Before you analyze your PCR products, let s take a look at the target sequence being explored.

More information

E.Z.N.A. mirna Kit. R preps R preps R preps

E.Z.N.A. mirna Kit. R preps R preps R preps E.Z.N.A. mirna Kit R7034-00 5 preps R7034-01 50 preps R7034-02 200 preps August 2011 E.Z.N.A. Micro RNA Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage and Stability...3 Preparing

More information

Using a Controlled Experiment to Identify Two Unknown Plasmids Edwin Braddy, River Ridge Middle/High School, New Port Richey, FL

Using a Controlled Experiment to Identify Two Unknown Plasmids Edwin Braddy, River Ridge Middle/High School, New Port Richey, FL INTRODUCTION To close the yellow note, click once to select it and then click the box in the upper left corner. To open the note, double click (Mac OS) or right click (Windows) on the note icon. Using

More information

Worm Genomic DNA Prep

Worm Genomic DNA Prep Worm Genomic DNA Prep CONTENTS: p2 Hawaiian Mapping Method (pooled samples) p4 Non-mapped samples (not pooled) p5 Genomic Prep from a few hundred worms (e.g. homozygous recessive lethal mutants) p6 Genomic

More information

DNA Purification for Case Transgene Pronuclear Injection Updated 2/26/08 RM

DNA Purification for Case Transgene Pronuclear Injection Updated 2/26/08 RM DNA Purification for Case Transgene Pronuclear Injection Updated 2/26/08 RM Materials: Stratagene StrataPrep DNA Gel Extraction Kit (Catalog #400766 or #400768) http://www.stratagene.com/products/displayproduct.aspx?pid=448

More information

AFFYMETRIX GENECHIP HYBRIDIZATION ANALYSIS (Updated: April 19, 2007) Experimental Organs:

AFFYMETRIX GENECHIP HYBRIDIZATION ANALYSIS (Updated: April 19, 2007) Experimental Organs: Date: AFFYMETRIX GENECHIP HYBRIDIZATION ANALYSIS (Updated: April 19, 2007) Experimental Organs: Note: This protocol is slightly modified from the general protocol for the biotin-labeled cdna generated

More information

OPPF-UK Standard Protocols: Mammalian Expression

OPPF-UK Standard Protocols: Mammalian Expression OPPF-UK Standard Protocols: Mammalian Expression Joanne Nettleship joanne@strubi.ox.ac.uk Table of Contents 1. Materials... 3 2. Cell Maintenance... 4 3. 24-Well Transient Expression Screen... 5 4. DNA

More information

ANG 111 Summer EXPERIMENT 1: CLONING June 23 July 14

ANG 111 Summer EXPERIMENT 1: CLONING June 23 July 14 ANG 111 Summer 2009 EXPERIMENT 1: CLONING June 23 July 14 Lab report for this experiment is due JULY 17, 2009 BY 5:00 pm. Late write-ups will be severely penalized. NOTE: IT IS IMPORTANT TO READ THESE

More information