enhance microcarrier separation and reduce cross-contamination risk Thermo Scientific HyQ Harvestainer BPC
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1 Thermo Scientific HyQ Harvestainer BPC enhance microcarrier separation and reduce cross-contamination risk Self-contained, single-use solution for harvesting and separating microcarrier cell cultures
2 Thermo Scientific HyQ Harvestainer BioProcess Container (BPC) Effective Efficient Economical
3 Closed single-use system reduces process times and simplifies collection New Technology in Microcarrier Separation and Cell Culture Harvest We utilize our expertise in single-use design and engineering to establish innovative technologies for use in cell culture-based vaccine production processes. The HyQ Harvestainer BPC enables separation of microcarriers and harvest of the cell culture supernatant in a single-step completely closed system. The Harvestainer BPC system features a dual chamber system comprised of a 200L BioProcess Container (BPC) that contains a 25L pillow-style BPC. This inner BPC, which is called the Thermo Scientific Microbarrier Labtainer BPC, has unique design features that enables the separation of cell culture supernatant from the microcarriers. Safe and Simple Using the Harvestainer BPC ensures full containment of the cell culture supernatant in a closed, single use system that eliminates the risk of cross-contamination. Additionally, the sophisticated design of this BPC reduces holdup volumes to increase recovery effiencies, giving you more product with less effort. Simple post-use disposal reduces cleaning costs and process cycle times.
4 How it works Transfer Port from Bioreactor Vent Adherent cells are cultured in a bioreactor using microcarrier beads Connect the Harvestainer BPC to the bioreactor via the transfer port Microbarrier Labtainer BPC Outer BPC Drain bioreactor contents directly into the Harvestainer BPC Microcarrier Beads Microcarrier beads are trapped in the Microbarrier Labtainer BPC, supernatant passes into the outer BPC Supernatant is ready for the next processing stage Bottom Drain Port Harvestainer BPC and microcarriers are ready for disposal Well Characterized and Customizable The Harvestainer BPC is comprised of well-characterized materials commonly used in single-use BPCs. Like all Thermo Scientific BPCs, the Harvestainer BPC is scalable and can be customized to readily integrate with existing equipment. It is designed to maximize product recovery and is built to the same quality standards found in all Thermo Scientific HyClone products. Go to ThermoScientific.com/Harvestainer and learn more
5 Flexibility and Scalability To ensure added flexibility, the Harvestainer BPC comes in a 200L size and is available with single or dual Microbarrier Labtainer BPCs. Multiple Harvestainer BPCs can be linked together in a manifold assembly, allowing for scale-up from process development to full-scale commercial production Single Microbarrier Labtainer BPC 1 4 Dual Microbarrier Labtainer BPC Line Description Lineset End Treatment 1 Microcarrier Transfer C-Flex - 1/2 ID x 1/8 Wall 2 Bottom Drain Harvest C-Flex - 1/2 ID x 1/8 Wall 3 Exhaust Line C-Flex - 1/4 ID x 1/8 Wall Filter: Pall Gas Filter 4 Microcarrier Transfer (only available on dual Microbarrier designs) C-Flex - 1/2 ID x 1/8 Wall Polycarbonate Quick Connect 1/2 Body (MPX), Polycarbonate Quick Connect Cap (MPX) Polycarbonate Quick Connect 1/2 Insert (MPX) Polycarbonate Quick Connect Plug (MPX) Polycarbonate Quick Connect 1/2 Insert (MPX), Polycarbonate Quick Connect Cap (MPX) Technical Specifications The Harvestainer BPC uses commonly used and well-characterized components eliminating the need for costly and time-consuming material qualifications. Ordering Information Part Number Description Harvestainer BPC Volume Microbarrier Volume (inner bag) SH Harvestainer BPC with Single Microbarrier Labtainer BPC 200L 25L SH Harvestainer BPC with Dual Microbarrier Labtainer BPCs 200L 25L x 2 Hardware Standard Products Part Number Description SV Polyethylene Drum, Bottom Drain Supplied with lid, latches and cap plug with rubber gasket SV Drum Dolly High density polyethylene, 24 3/8 D x 7 1/8 H thermoscientific.com/bioprocessing
6 Integrated Solutions for BioProduction Single-Use Mixers (S.U.M.) A variety of options up to 2000L for both upstream and downstream applications. Powdered & Liquid Media We offer both custom manufacturing and a full range of standard media and supplement products. BioProcess Containers (BPCs) A variety of configurations up to 2000L for liquid harvest, storage, and transportation. Serum Our serum is the industry standard for consistent quality and reliability. Open-Architecture Single-Use Bioreactors (S.U.B.) L bioreactors capable of integrating with an existing control system. Buffers & Process Liquids Custom and standard buffers and process liquids, including WFI Quality Water. Cell Culture and BioProcessing In the Americas and Asia 925 West 1800 South Logan, Utah USA Tel: +1 (435) Toll-Free: +1 (800) Fax: +1 (435) thermoscientific.com/harvestainer In Europe Industriezone III, Industrielaan Erembodegem Belgium Tel: Fax: Scan with a QR reader or visit YouTube to watch the Harvestainer BPC video Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. SM0546
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