University Hospital Southampton NHS Foundation Trust G1.12 LABORATORY MEDICINE G1.12 Sample storage Disposal v5
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1 G1.12 Sample storage v5 G1.12 SAMPLE STORAGE & DISPOSAL Clinical significance Samples must be stored under the correct conditions and for an appropriate amount of time. Where possible sample tracking systems should be used to allow rapid retrieval. Instructions Samples should be stored according to the table below in this document. The table includes section, sample, location, tracking/date order, condition, area or fridge/ name, retention and disposal. All samples MUST be disposed of following Safety SOP S1.25. Security All specimens are stored in the fridges and s within secure area where only authorized staff have access through security swipe cards or digital padlock. Risk assessment All samples are treated as POTENTIAL BIOHAZARDS hence, oratory coats, gloves and eye protection must be worn at all times when handling any open samples. Revision 5 Page 1 of 14 IF THE FIRST PAGE OF THE PROCEDURE DOES NOT HAVE RED APPROVED METHOD STAMP IN THE FOOTER THAT PROCEDURE PRINTED COPY IS UNCONTROLLED.
2 G1.12 Sample storage v5 Automated Haematology Track samples Blood (On machine and bench cleared to fridge each morning) Fridge Front load samples TS-10 (RT initially then moved to Fridge) 4C Forensic Tox Cold Room 36 hours Yellow clinical Blood Auto Haem TrackIT RT Auto haem 36 hours Yellow clinical Slides Blood Auto Haem Date Room temp Auto haem 1 week Disposed into sharps bin Slides Marrow Bench Date Room temp Medics room on Level C 1Year Disposed into sharps bin Slides Marrow Date Off-Site Storage Not Applicable Indefinitely Off-site storage CSF samples CSF Auto haem Date Room temp Auto haem 1 week Place in hard plastic containers (previous reagent bottles) and then disposed off in the waste bag. Revision 5 Page 2 of 14
3 G1.12 Sample storage v5 Haemostasis & Special Techniques Routine Samples e,g. WARF, HEP & CS Special Test Samples e.g. Thrombophilia, VWS, factor assays Long-Term Special test samples e.g. First time Thrombophilia, VWS, factor assays Specialised Haematology samples e.g haemoglobino pathy Blood Stored Plasma Stored Plasma Blood Sample racks -70 C Freezer -70 C Freezer Sample racks TrackIT Room temp Haemostasis & Special techniques department bench TrackIT -70 C Haemostasis & Special techniques department 1 (-70 C) TrackIT long term rack Track-IT or Sysmex TS C RT then Forensic Tox Cold Room room 4 C Haemostasis & Special techniques department 1 (-70 C) Forensic Tox Cold Room room 4 C 24 hours Yellow clinical 6 months Yellow clinical 1 calender year 48 hours after testing Yellow clinical Yellow clinical Revision 5 Page 3 of 14
4 G1.12 Sample storage v5 Blood Transfusion Section Specimen All Samples All samples LD16 TrackIT 2-8C Reagent Fridge 10 days Clinical waste bins Rare Plasma -70 C Indefinitely Clinical waste bins Antibodies Freezer Specimen Reception Surplus samples Surplus timed urine Surplus random urines/fluids TrackIT -70 C Haemostasis & Special techniques department (-70 C) Blood Reception None Room temp for 24 hr then 4c Foster fridge/l 1 week Yellow clinical Urine Reception None Room temp Urine aliquoting area 24 hours Sluice Urine Reception None Room temp then 4c CSF Samples CSF Reception None Room temp then 4c Foster fridge/l 1 week Yellow clinical Foster fridge/l 1 week Special CSF autoclave bin Revision 5 Page 4 of 14
5 G1.12 Sample storage v5 Forensic Toxicology Routine Urine Cold room TrackIT 4C Forensic Tox Cold Room room 4 C Post Mortem All sample s Freezer room Chain of custody form Post mortem All sample s Tox area Chain of custody form 1 month Yellow clinical -20oC Freezer room 2 years Yellow clinical 4 C LEC Medical and LEC Technical fridge Until analysis complete First stored 2 years then autoclave bins Trace Elements All Tests Blood/Plasma/U rine LD60 (Fridge overnight) Manual, Sectional SOP TE 1.1 Room temp (4oC over night) LD60-20C 1 Month Clinical waste bins Revision 5 Page 5 of 14
6 G1.12 Sample storage v5 Specialist Biochemistry Routine automated / manual samples Serum/ urine Freezer Room Blood spots Blood spots Manual area LC 38 Faecal Elastase HbA1c Trackit.-20C Freezer Room/Freezer 1, Freezer 2, Freezer 3 Date Faeces LD10 Date -20 C EDTA Whole blood Room temp Area LC 38 Trackit 2 8 C Under bench cupboard white asset No. AB0027 in room LD10 Fridge 1 1 month Yellow clinical 2 months Yellow clinical 2 weeks Yellow clinical 2 days Yellow clinical Revision 5 Page 6 of 14
7 G1.12 Sample storage v5 Automated Biochemistry Track samples Front load samples CSF primary samples Blood/Urine/Flui ds Blood/Urine/Flui ds / CSF Stock yard Track 4C Track fridge 24hours Yellow clinical Fridge Track it 4C Foster fridge/l 24 hours Yellow clinical CSF Reception None Room temp then 4c Foster fridge/l 1 week Special CSF autoclave bin Critical Care Laboratory Blood Gases Blood Gases Bench Manual tracking Room Temp Not Applicable N/A Disposed into sharps bin after analysis All other Tests All other tests Fridge 4C Manual tracking 4C CCL fridge A 14 days Disposed into sharps bin after 14 days Revision 5 Page 7 of 14
8 G1.12 Sample storage v5 Chromatography Section Specime n 5HIAA Urine Tox & Met Metanephrines Urine Tox & Met HMMA Urine Tox & Met Vitamins A&E, Serum Tox & Met Amino acids Serum Tox & Met Amino acids CSF Tox & Met Amino acids Urine Tox & Met Organic acids Urine Tox & Met Ketones Urine Tox & Met Organic acids Urine Tox & Met Ketones Urine Tox & Met Phenylalanine & Bloodspot Tox & Met Tyrosine Track it 2-8 C LD 32 Cold room 1 month Clinical waste bins Track it 2-8 C LD 32 Cold room 1 month Clinical waste bins Track it 2-8 C LD 32 Cold room 1 month Clinical waste bins Track it 2-8 C LD 32 Cold room 1 month Clinical waste bins Track it <-60 C LD 39 New Brunswick ultra low temperature Track it <-60 C LD 39 New Brunswick ultra low temperature Track it <-60 C LD 39 New Brunswick ultra low temperature 1 month Clinical waste bins 1 month Clinical waste bins 1 month Clinical waste bins Track it <-60 C LD 39 New Brunswick ultra low temperature 1 week Clinical waste bins Track it <-60 C LD 39 New Brunswick 1 week Clinical waste bins ultra low temperature Track it <-18 C LD 39 liebherr comfort 1 month Clinical waste bins Track it <-18 C LD 39 liebherr comfort 1 month Clinical waste bins Date order Room temp LD 33 bench 1 month Clinical waste bins Revision 5 Page 8 of 14
9 G1.12 Sample storage v5 Chromatography Section Specime n 17- Hydroxyprogester one Bloodspot Tox & Met Acyl carnitines Bloodspot Tox & Met 25- Serum Tox & Met Hydroxyvitamin D Androstenedione Serum Tox & Met 17- Serum Tox & Met Hydroxyprogester one Testosterone by LC-MS-MS Reducing substances Serum Faeces Tox & Met Wash-up corridor Date order Room temp LD 33 bench 1 month Clinical waste bins Date order Room temp LD 33 bench 1 month Clinical waste bins Track it <-18 C LD 39 liebherr comfort Track it <-18 C LD 39 liebherr comfort Track it <-18 C LD 39 liebherr comfort 1 month Clinical waste bins 1 month Clinical waste bins 1 month Clinical waste bins Track it <-18 C LD 39 liebherr comfort 1 month Clinical waste bins Date order <-18 C Proline 1 month Clinical waste bins Revision 5 Page 9 of 14
10 G1.12 Sample storage v5 Manual Biochemistry 4ºC Samples 4ºC Universals -20ºC Samples 4ºC CSF Samples (Not Tracked) Calculi Blood/Urine/Flui ds Urine/Fluids Forensic Tox Cold Room room 4 C Forensic Tox Cold Room room 4 C TrackIT 4ºC Forensic Tox Cold Room room 4 C TrackIT 4ºC Forensic Tox Cold Room room 4 C 1 month Yellow clinical 3 month Yellow clinical Serum/Urine Freezer TrackIT -20ºC VW 3 month Yellow clinical CSF Track fridge None Track fridge Track fridge 1 month Yellow clinical Renal / Gall Bladder / other LD24 None Ambient LD24 under Bench 1 year Yellow clinical Revision 5 Page 10 of 14
11 G1.12 Sample storage v5 Immunology Section Flow cytometry Flow cytometry Flow cytometry Flow cytometry o C and approx 1 month. Specimen Blood LC41 Lab number 2-8C Fridge 3 1 week Yellow clinical Bone marrow LC41 Lab number 2-8C Fridge 3 1 week Yellow clinical CSF LC41 Lab number 2-8C Fridge 3 1 week Yellow clinical Pleural fluid LC41 Lab number 2-8C Fridge 3 1 week Yellow clinical Blood/Plasma Williams fridge TrackIT 4C Williams fridge 30 Days Yellow clinical Revision 5 Page 11 of 14
12 G1.12 Sample storage v5 Immunology Section 20 o C approx 1 month. o C approx 3 months. -20 o C approx 5 months. Longterm storage samplesindefinately Specimen Blood/Plasma Blood/Plasma Blood/Plasma Blood/Plasma Williams Williams fridge Williams Scandinova Freezer 1 TrackIT -20C Williams 30 Days Yellow clinical Sequential numbers in blocks of 100 (Manual) Sequential numbers in blocks of 100(Manual) Labelled container and no. Entered in dedicated book (Manual) 4C Williams fridge 30 Days Yellow clinical -20C Williams 30 Days Yellow clinical -20C Scandinova Freezer 1 30 Days Yellow clinical Revision 5 Page 12 of 14
13 G1.12 Sample storage v5 Revision 5 Page 13 of 14
14 G1.12 Sample storage v5 Molecular Pathology. Please also refer to MP1.9 Molecular Pathology Molecular Pathology Molecular Pathology Molecular Pathology Molecular Pathology Blood / Bone marrow, non FFPE primary samples Lab Order of receipt in 4ºC Pre PCR fridge Min one month Autoclave FFPE Lab None Room temp Pre PCR Until result validation DNA Lab TrackIt 4ºC while in use then in for long term storage Cell lysates Lab None, but TrackIt for MRD back up samples Pre PCR fridge and Approx 3 years except for Transplant pre/donor samples must be kept indefinitely -80 ºC Freezer BCR-ABL approx 3 months, SLN lysates until sent to BioBank at Uni of Southampton Returned to requesting oratory Autoclave Autoclave cdna Lab Date order >-15ºC Freezer Approx 6 months Autoclave Revision 5 Page 14 of 14
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