mirrorball accelerating antibody discovery
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- Norma Oliver
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1 mirrorball accelerating antibody discovery
2 meet mirrorball efficient antibody discovery is within your reach TTP Labtech s mirrorball fluorescence cytometer revolutionises the productivity of antibody discovery. Simple and economical workflows decrease hands-on time, remove throughput barriers and alleviate budget constraints to make light work of hit identification and characterisation. Confidently progress lead candidates on time using this reliable and versatile solution. simple productive economical reliable versatile
3 is TTP Labtech s mirrorball a flow cytometer, or ELISA reader? Neither, mirrorball uses TTP Labtech s industry-proven laser scanning fluorescence cytometry to provide HTSfriendly workflows that deliver gold standard data quality. efficiency through process delivering innovation mirrorball versus ELISA mirrorball versus flow cytometry no-wash protocols minimise hands-on time for cell- and bead-based immunoassays multiplex up to 5 targets to save costs and time conserve precious sample (10 µl / test) for downstream hit characterisation in-situ adherent cell analysis eliminates cell suspension protocol step significantly decreased operational and maintenance costs hit characterisation through image-based functional assays non-fluidics approach: no delays from system clogging; no potential carry-over whole well images permit visual confirmation of results for assured decision-making automation-friendly workflows increase throughput capacity faster plate read times: 12 mins / plate no-wash screening protocol A simple two-step addition process is all that is required for bead or cell-based homogeneous immunoassays. add 10 µl of sample, 10 µl of cells or beads and detection reagent to each well incubate read in 12 mins
4 work the way you want to within antibody screening deliver decision-making data within budget and on time mirrorball is suitable for hybridoma, phage display, B-cell cloning, antibody fragments, peptides, scfv and Fab fragment screening. negative positive fluorescence don t compromise data integrity with mirrorball: choose the convenience of a bead-based approach where appropriate preserve the epitopes of cell-surface antigens with cell-based screens image adherent, or suspension cells in-situ no-fluidics approach: low affinity and fast koff interaction detection zero carry-over between wells no delays due to system clogging increase productivity with mirrorball: streamlined no-wash assay protocols multiplex: combine hit identification with non-selective and non-specific counter-screens reliable walk-away automation for multi-plate screening work to budget with mirrorball: economical use of precious sample and reagents low cell and bead requirements / well zero daily / routine maintenance and running costs work the way you want to within lead optimisation and characterisation progress lead candidates confidently: species cross-reactivity competition assays isotyping Kd / Bmax determination functional applications: cell-based assays: cell health, cell cycle, proliferation, cell signalling, antibody internalisation, virus neutralisation bead-based soluble protein assays: cytokines, hormones, growth factors neutralising antibody Virus neutralisation assay: whole well fluorescent images from mirrorball showing nuclei (FL1, blue) and virus infected cells (FL-2, green). mirrorball offers great versatility for performing a wide range of downstream lead optimisation and characterisation tests.
5 Cellista software: click to results in just12 minutes mirrorball s Cellista software is powerful yet approachable For antibody screening, getting started could not be simpler: choose from pre-loaded assay templates and start acquiring data. On-the-fly analysis gives you decision-making data in just 12 minutes per 96-, 384- or 1,536-well microplate. Capitalising on mirrorball s image-based approach Cellista offers great versatility to address a wide variety of functional applications. Cellista extracts multi-parameter data from individual beads, cells or plaques. development teams will love: the removal of adoption barriers normally associated with image analysis hit identification through easy gating of histograms and scatter plots assured decision-making with whole well images that provide visual confirmation of results intensity and morphological output measurements no requirement for specialist operators or intensive training the end of training is the beginning of our partnership TTP Labtech strives to offer customers the very best in application and instrument support services. We offer easy set-up and ongoing support from the very first meeting. To further enhance your productivity, assay development and technology transfer services are available upon request. TTP Labtech instruments typically have a life cycle in excess of 10 years, proving a great return on investment. an effortless route to automation For no-fuss automation, mirrorball can be supplied with a fully-integrated Thermo Scientific Orbitor with capacity for 80 plates. mirrorball can also be integrated with automation workcells from many major vendors including: PAA HighRes Biosolutions Wako Automation Agilent Technologies CyBio Beckman Coulter main application laser excitation detection fluorescence detection scan area standard data format mirrorball target-based biologics screening choice of 405, 488 or 640 nm photomultiplier tube (simultaneous) 2, 4 or 5 channels plus laser scatter whole well as standard CSV file plate compatibility 96, 384 and 1,536 scan and analysis time 12 minutes per plate (irrespective of well density)
6 sol-r coded beads: make light work of ELISA watch your costs go down and your productivity go up TTP Labtech s sol-r coded beads represent a range of screening reagents for multiplexed assays. Designed for use with mirrorball, they offer a powerful alternative to ELISA. simple productive no-wash protocols up to 5 codes for multiplexing economical screening friendly price point for antibody screening: enhance productivity through the combination of target specificity and selectivity binding protocols into one assay. for soluble protein determination: enhance productivity through quantifying multiple proteins in one immunoassay. sol-r microplate clarity for image-based immunoassay and phenotypic screening TTP Labtech s sol-r microplate is an affordable, high-quality 384-well plate that has been designed to optimise your mirrorball or acumen fluorescence cytometer. It is equally compatible with microscopebased high content imaging devices. Engineered to ensure confidence in your data, the sol-r plate offers: reliable versatile consistent binding capacity across codes immobilise whole proteins, fragments or antibody optimised design for reliable focus and well positioning plate flatness and rigidity, without dimples available sol-r kits Any combination of codes 1-5 may be brought together into sol-r bead kits. Kits are optimised for 384-well plates, but protocols for 96-well and 1,536-well plates are also available. TTP Labtech s sol-r beads may be purchased in volumes for dispensing into 1, 5, 20, 50 and 100 assay plates. <3% CVs across the microplate in QC tests high-grade optical-quality film for clarity available in sterile and TC treated (cat # ), or non-sterile and non-tc treated (cat # ) formats
7 get in touch TTP Labtech Ltd Melbourn Science Park Melbourn Hertfordshire SG8 6EE United Kingdom +44 (0) TTP Labtech Inc One Kendall Square Suite B2303 Cambridge MA United States +1 (617) TTP Labtech India Private Limited SRS Tower 212, 2nd Floor Sector 31, 14/5 Mathura Road Faridabad (NCR Delhi) India
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