BR-9516B. SNP genotyping analysis for medium to ultra-high throughput. GENOMELAB TM SNPSTREAM GENOTYPING SERIES
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1 BR-9516B SNP genotyping analysis for medium to ultra-high throughput. GENOMELAB TM SNPSTREAM GENOTYPING SERIES
2 Where power meets flexibility. The GenomeLab SNPstream Genotyping System provides an automated, scalable system capable of analyzing thousands of genotypes a minute to millions of genotypes per day. Combined with multiplexing capabilities, experimental layouts and operational flexibility, enables you to process a wide range of studies. SNPstream is a robust research solution designed to deliver your results faster and with less effort, while solving problems more thoroughly. Simple and Scalable SNP Genotyping. SNP genotyping has never been easier. The GenomeLab SNPstream features a simple protocol... it s as easy as 1, 2, 3. The system provides a scalable solution that meets medium- to high-throughput needs, by processing a range of 4,600 to over 3,000,000 genotypes per day. High Fidelity / Accuracy Single-base primer extension technology delivers accuracy rates of greater than 99%. This patented method the gold standard is the most validated technique available. Our system software allows 100% data QC. Sensitive Only 2 ng of DNA is required per sample for either the 12-plex or 48-plex assay. This equates to 0.04 ng DNA required per genotype. Scalable Throughput Flexible multiplexing and advanced automation provide the capability to process a broad range of 4,600 to over 3,000,000 genotypes per day. Plate-handling robotics increase throughput while providing walk-away operation. Simple An integrated bar code reader enables accurate sample tracking and reporting. Our standardized reaction conditions and simple steps mean no optimization is necessary. 12-Plex 48-Plex 12-plex or 48-plex SNP Genotyping. The GenomeLab SNPstream is an automated multiplexed system that can process either 12 or 48 SNPs in each well of an arrayed 384-well plate. This system operates at a consistent cost per sample, regardless of run throughput, allowing users to cost-effectively zero in on research targets with low-throughput studies as well as conduct high-throughput analyses.
3 In a bench-top design, the GenomeLab SNPstream Genotyping System utilizes single-base primer extension technology in a tagged fluorescent assay to deliver an automated, scalable solution for your medium to high throughput analysis. It comes with reagents, software and instrumentation developed to work optimally together, as well as a CCD-based imager. Flexibility All common plate layouts are supplied enabling analyses. Scalable assay automation, automated data collection, data analysis, and auto QC provides a flexible and fully automated solution from DNA to genotype calls. Cost-effective By lowering PCR expenses and improving efficiency, the 12-plex and 48-plex power built into the GenomeLab SNPstream reduces cost per genotype and per sample. Multiple DNA Sources Blood, tissue, cell-culture, buccal swab, etc. Multiple Species Human, mouse, cow, drosophila, plant, microbes, etc. Service & Assay Support To help you always get the most out of your Beckman Coulter equipment, we maintain a worldwide reputation for on-line, on-call and on-site support resources. And our experienced applications scientists are available to help you achieve optimum results.
4 A complete genotyping solution. The single-base primer extension technology integrated into our GenomeLab SNPstream Genotyping System delivers the answer to all your genotyping needs with consistent, reliable, reproducible results, and accuracy that surpasses DNA sequence studies. This user-friendly technology provides data that s easy to interpret, as well as the versatility to adapt to multiple study formats. SNP Genotyping as easy as 1,2,3 Simple, accurate, flexible primer extension. Same simple chemistry, two levels of multiplexing. After the SNP primer is hybridized to the pre-amplified DNA, the primer is extended by a labeled terminating base at the target SNP site. The extended primer is then captured on the SNPware Tag Array plate and detected. Step1: Hybridize Step 2: Extend Step 3: Detect With millions of genotypes generated using single-base primer extension, the GenomeLab SNPstream Genotyping System has been proven to deliver the accurate and reliable data that today s laboratories demand. Two-levels of multiplexing chemistry (12-plex or 48-plex) are assayed under the same standardized conditions. 12-PLEX / 48-PLEX GENOTYPING SUCCESS RATE > 95% ACCURACY > 99% Tag array method. Multiplexed reactions are spatially resolved on a SNPware Tag Array plate. Each of the 384 wells in a plate contain 16 or 52 unique oligonucleotides of a known sequence, or tag. Each tag on the plate is complementary to one of the tags of the12 or 48 extension primers, plus four controls to ensure accuracy. After transfer to the Tag Array plate, each SNP hybridizes to its complementary tag. SNPs are then identified by their position in the well. SNPware Reagent Kits (12- or 48-plex). Our preformulated, ready-to-use SNPware Reagent Kits are optimized for effortless and robust single-base primer extension reactions. The kits include ready-to-use reagents for simple addition reactions, all performed in the original PCR plate. No precipitation or filtration steps are required.
5 Manual The GenomeLab SNPstream s assay setup is easily achieved by manual pipetting. Our assay setup is compatible with either manual operation or different automated solutions. Biomek 3000 Ideally suited to meet the needs of low- to medium-throughput research applications. Biomek NX Available in multiple configurations and provides a high degree of flexibility with medium throughput. Biomek FX Offers the highest capacity deck space available and the most flexibility in device integration. Provides total application solutions with either single- or dual-bridge configurations that accommodate a variety of pipetting options. Autoprimer.com This innovative on-line resource takes the uncertainty out of assay design by providing fast, automated primer design with tag assignments. Just upload or enter your sequence information at select the level of multiplexing and our state-of-the-art algorithms will instantly design robust, best-fit multiplex PCR and extension primers. By designing panels to work under one standardized PCR condition as well as extension conditions, Autoprimer.com software takes care of the optimization so you don t have to.
6 The GenomeLab TM advantage. To provide researchers with the most comprehensive and efficient solutions, the GenomeLab SNPstream Genotyping System and all our genetic analysis solutions integrate leading-edge systems, software and chemistries with a targeted focus on genetic research. It s our way of giving you easy access to a full spectrum of powerful genetic analysis tools that help accelerate discovery. Easy sample setup. User-friendly software makes setting up even the most complex assays quick and easy. From a single point of entry, you can input and customize every facet of setup. You can also choose to cut and paste existing information, utilize file transfer information, or directly upload from Autoprimer.com. Automated image processing. The data is automatically imaged and processed into genotype calls, with the location and intensity of a spot within the well determined for each wavelength. A genotype call is then made based on the relative fluorescent intensities of each spot. The information is stored in the database and can be easily accessed for further use. Automate data QC with user-definable parameters. These AutoQC filters can be modified to meet your need and automatically qualify genotyping data. Results you can visualize and use. Data results are presented in various formats that allow users to make a pass/fail review of all the data. The system s flexibility allows for visual review and manual adjustment of the genotype clusters to maximize accuracy and throughput, while reducing the potential need to repeat assays. SNP genotype data can be easily exported in simple text format to review and prepare reports, or imported into a downstream analysis program.
7 Seamless workflow from raw data to genotype calls. Genotype summary report. SNP genotype data can be easily exported in simple text format to review and prepare reports, or imported into a downstream analysis program. Analysis is a critical step in the genomics process, and involves determining sequences, identifying sequences and genetic elements, genotyping, gene expression, detecting mutations, quantitative expression, and viral load. TM GENOMELAB FROM TISSUES TO TARGETS. Identify Isolate The GenomeLab SNPstream Genotyping System is an important part of a comprehensive set of interrelated GenomeLab solutions specifically designed to accelerate genetic research. By encompassing virtually every step of the process, our flexible and highly efficient systems function as an extension of your thinking, helping you make important research decisions faster and with more confidence than ever before. Prepare Analyze Evaluate Validate Scatter plot and user-friendly interface for data review. Advanced software permits a quick macro view of entire plate s data. By giving you advanced, interactive ways to look at data, our specialized tools let you explore the genotype clusters to maximize accuracy and throughput, while reducing the potential need to repeat assays.
8 Identify Isolate Prepare Analyze Evaluate Validate The SNPstream and all our GenomeLab offerings are an important part of a broad continuum of Beckman Coulter products, including DNA sequencing, capillary electrophoresis, fragment analysis, automated liquid handling, centrifugation, ultracentrifugation, electrochemistry, flow cytometry, HPLC, integrated core systems, microarrays, particle characterization, scintillation counting, and spectrophotometry. For information on our comprehensive line of GenomeLab systems, please contact your local Beckman Coulter representative or visit our web site at The PCR process is covered by patents owned by Roche Molecular Systems, Inc. and F. Hoffman-La Roche, Ltd. All trademarks are the property of their respective owners. For Research Use Only: Not for use in diagnostic procedures. Flow cytometry Automated fluorescence microscopy Cell viability analysis Automated liquid handling General purpose centrifugation High performance centrifugation Ultracentrifugation HPLC Automated liquid handling Centrifugation Chemistries Spectrophotometry Capillary electrophoresis Microarray technology Software informatics Automated liquid handling Microarray technology Lab developed protocols Research protocols ORDERING INFORMATION GenomeLab TM SNPstream Genotyping System Part No (12 plex), A24136 (48 plex) Flexible, multiplexed, medium to ultra-high throughput system capable of processing from 4,600 to over 3,000,000 genotypes per day. Automated robotic plate handler. Integrated bar code reader. CCD-based imager. Data Analysis workstation with integrated server. Comprehensive software suite provides a seamless workflow, from primer design to 384-well plate setup, analysis of image data, and genotype calls. Specifications Weight 157 lb (71.2 kg ) Height 22 in (56 cm) Width 24 in (61cm) Depth 50 in (127cm) Power VAC,20AMax.,50/60Hz Excitation One class III ion laser Class1 laser hazard SNPware Reagent Kits Part No. 12-plex A16997 SNPware 12-plex Core Reagent Kit SNPware 12-plex G/A ExtensionMix SNPware 12-plex T/C ExtensionMix SNPware 12-plex C/A Extension Mix SNPware 12-plex T/G ExtensionMix SNPware 12-plex T/A ExtensionMix SNPware 12-plex C/G Extension Mix 48-plex A23953 A17296 A17292 A17288 A17276 A17282 A17267 SNPware 48-plex Core Reagent Kit SNPware 48-plex A/G ExtensionMix SNPware 48-plex T/C ExtensionMix SNPware 48-plex C/A Extension Mix SNPware 48-plex T/G ExtensionMix SNPware 48-plex T/A ExtensionMix SNPware 48-plex C/G Extension Mix Based on gold standard single-base primer extension technology. Complete reagents post PCR clean-up. Pre-arrayed plates for simple SNP reaction processing. SNPware reagents kits pre-formulated and ready to use. 0.04ng DNA per genotype. Standardized reaction conditions, for reliable assays. Volumes compatible with manual or automated processing. Developing innovative solutions in genetic analysis, drug discovery, and instrument systems. Beckman Coulter, Inc N. Harbor Boulevard, Box 3100 Fullerton, California Sales: Service: Telex: Fax: Worldwide Biomedical Research Division Offices: Australia (61) Canada (905) China (86) Eastern Europe, Middle East, North Africa (41) France Germany Hong Kong (852) / Italy Japan Mexico Netherlands Singapore (65) South Africa, Sub-Saharan Africa (27) /5 Spain Sweden Switzerland Taiwan (886) Turkey U.K U.S.A B CB Beckman Coulter, Inc. Printed in U.S.A. on recycled paper.
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