Nuclear Receptor Bio-Assays

Transcription

1 Nuclear Receptor Bio-Assays Kun Bi, Ph.D. Senior Staff Scientist Gerry Pelanek Associate Director New Markets & Business Development Joint Meeting of California and Water Reuse Research Foundation Project Teams November 10, 2011

2 Agenda Who are we NR Portfolio Overview Specific Assays and Data Prior Environmental Toxicity Studies Water Testing Application Scenarios 2 11/9/2011 Life Technologies Proprietary and confidential

3 Who We Are Life Technologies Carlsbad, CA / Corporate Headquarters Globally rendering discoveries and developments that make life better Corporate Fast Facts Presence 160 Countries 2010 Net Sales $3.6B Employees ~ 11,000 Product Types > 50,000 Water Saved 2008 vs % Corporate Responsibility Magazine 100 Best Corporate Citizens List Life Technologies Madison, WI Location Serves Bio/Pharma Drug Discovery with validated assays and services Developed and manufactures Life s nuclear receptor assay portfolio ISO 9001 Quality Certification since 2008 Awarded ToxCast Contract August 2011 OSHA s highest rating of safety practices 3 11/9/2011 Life Technologies Proprietary and confidential

4 Implementation Support Field Support Network Technical Sales Specialists (TSS) Field Application Specialists (FAS) Regionally located Centralized Technical Support Team Dedicated support to our portfolio Staffed with Ph.D. scientists Phone and support Custom Development Services Our experts solving your challenges Dedicated team of scientists and project managers Proven track record since 2005 SelectScreen Profiling Services Our HTS lab instead of yours Automation and personnel providing fast, accurate and consistent results Technical Seminars/Webinars Scientist-to-scientist engagement Custom Knowledge & Skill Training Build assay competency efficiently Lecture and hands-on training application E.g. - Hepatocyte Expert Program We provide a spectrum of support options to meet customer needs 4 11/9/2011 Life Technologies Proprietary and confidential

5 Our Nuclear Receptor Platform Question It Answers Does the compound bind and potentially affect NR function? Assay Type Features / Benefits Available As: Product Service LanthaScreen TR-FRET Competitive Binding Assays >30 Biochemical NR Screening Assays Detects if compound binds to NR Simple mix and read no cell handling skills required What is the compound s functional impact? GeneBLAzer Cell-Based Nuclear Receptor Assays Quantitativelymeasures cell response Elucidate agonist or antagonist effect Option for Assay Ready cells SelectScreen NR Profiling Service >20 Functional NR Cell-Based Assays Easiest of all we do it for you! Utilizes our functional cell-based assays High Throughput Screening Service Broad options available for evaluating Nuclear Receptor impact 5 11/9/2011 Life Technologies Proprietary and confidential

6 Nuclear Receptors 48 known human proteins Agonist binding to a nuclear receptor results in a conformational change in the receptor which activates the receptor Transcription factors together with coregulators to control the expression of genes important for Development Homeostasis Metabolism Six steroid hormone receptors for sensing the presence of hormones and endocrine disrupting compounds Disruption of normal NR action can cause significant functional changes 6 11/9/2011 Life Technologies Proprietary and confidential

7 Biochemical Assays: NR LanthaScreen TR-FRET Competitive Binding PPARγ Compound Binding Profiles TR-FRET ratio 520nm/495nm GW1929 GW9662 rosiglitazone GW7647 troglitazone pioglitazone arachidonic acid GW0742 Key Features: [Compound] (nm) Detects compound binding (cannot determine agonists and antagonists) Simple mix and read format TR-FRET to minimize optical interference from fluorescent compounds or particulates High throughput screening (HTS) compatible Assay Configurations of Interest: Kits for: ERα, ERβ, GR, PXR, PPARα, PPARδ, PPARγ Tool box reagents for: AR, PR, VDR Simple format providing Yes/No impact potential, no cell handling skills required 7 11/9/2011 Life Technologies Proprietary and confidential

8 Profiles of Known Compounds for AR, PR, ERα and ERβ TR-FRET ratio 570nm/546nm AR LBD-GST TR-FRET Binding Assay [compound] (nm) IC 50 (nm) dihydrotestosterone 1.1 testosterone 3.7 R progesterone 400 estradiol 185 cortisol TR-FRET ratio (570nm/546nm) PR LBD-GST TR-FRET competitive binding assay [compound] (nm) R5020 (0.49 nm) mifepristone (0.52 nm) norethindrone (0.55 nm) progesterone (1.33 nm) 21-hydroxyprogesterone (8.94 nm) corticosterone (35.5 nm) testosterone (73.6 nm) dexamethasone (314 nm) TR-FRET ratio (520nm/490nm) ERα LBD-GST TR-FRET Binding Assay Ligand (nm) Estradiol (0.31 nm) DES (0.49 nm) Estrone (1.34 nm) Ethynyl Estradiol (0.45 nm) Genistein (11.9 nm) 4-OH Tamoxifen (0.50 nm) Raloxifene (0.50 nm) Tamoxifen (10.3 nm) TR-FRET ratio (520nm/490nm) ERβ LBD-GST TR-FRET Binding Assay 0.20 Estradiol (0.41 nm) DES (0.64 nm) 0.15 Estrone (2.6 nm) Ethynyl Estradiol (0.91 nm) 0.10 Genistein (0.90 nm) 4-OH Tamoxifen (0.79 nm) 0.05 Raloxifene (1.4 nm) Tamoxifen (22.9 nm) Ligand (nm) Sensitive assays detect compounds with wide range of affinity 8 11/9/2011 Life Technologies Proprietary and confidential

9 GeneBLAzer Cell-Based Nuclear Receptor Assays Advantages: Quantitatively measures compound s functional impact on cells Lower background and less interference from other NRs & crosstalk Functional read-out for ligand and receptor interaction Available Formats: Cryopreserved stable cell lines For expansion and continuous culture Cryopreserved assay-ready cells Thaw and plate cells run assay Quantitative functional live cell assay compatible with HTS and toxicity multiplexing 9 11/9/2011 Life Technologies Proprietary and confidential

10 How Do You Screen with Our NR Cellular Assays? Plate containing compounds Blue & Green Add cells, in media Incubate Read fluorescence in a standard plate reader! Add detection solution Make detection solution by mixing together selected substrate, and solutions B & C Simple, HTS-compatible workflow: plate, mix, add and read! 10 11/9/2011 Life Technologies Proprietary and confidential

11 Multiplexing Functional and Cytotoxic Effects With ToxBLAzer Substrate ToxBLAzer Use and Benefits: Simple blends into functional assay s workflow; high throughput screening compatible Efficient measure functional and cytotoxicity effects from the same well at the same time. Powerful Identify false positives due to toxicity and assay errors that result in extremely low cell density. Functional Readout Cytotoxicity Readout Stimulated D1-CRE-bla-CHO-K1 Cells with ToxBLAzer Add cells, in complete media w/ 2% serum Compounds No additional reagent manipulations! Add 6X detection solution Incubate Blue, Green & Red Read fluorescence in a standard plate reader! Make 6X loading solution by mixing together ToxBLAzer solution A, and solutions B & C Concurrently assess functional impact and flag cytotoxic effects. Life also has a comprehensive suite of cell health assays /9/2011 Life Technologies Proprietary and confidential

12 Cellular Assay Compound Profiles blue/green ratio ERα log compd [nm] diethylstilbestrol genistein estriol estrone ethynylestradiol blue/green ratio ERβ log compd [nm] Compound (EC50, nm) Agonists ERα ERβ 17β-Estradiol Diethylstilbestrol Genistein Estriol Estrone Ethynylestradiol Antagonists blue/green ratio OH-tam + 0.2nM E2 tam + 0.2nM E2 RU nM E2 blue/green ratio Compound (IC50, nm) ERα ERβ 4-OH-tamoxifen Tamoxifen log compd [nm] log compd [nm] RU For compound profiles for AR, PR, TR and PPARγ, see appendix. For additional compound profiles for ERs, see Wilkinson, et al. (2008) Journal of Biomolecular Screening 13:755. Provides sensitive, quantitative functional assessment for agonists or antagonists 12 11/9/2011 Life Technologies Proprietary and confidential

13 Applications of Our NR Cellular Assays in Environmental Toxicity Studies Pilot Phase Study EPA National Center for Computational Toxicology Huang, et al. (2011) Environmental Health Perspectives 119:1142. Prior Tox21 program evaluations demonstrated assay feasibility for environmental toxicity applications 13 11/9/2011 Life Technologies Proprietary and confidential

14 Water Quality Testing Scenarios with our NR Assays Scenario 1: Multiple Pass Triage and Hit Follow-Up Pass 1: Yes/No Triage LanthaScreen TR-FRET Competitive Binding Assays Pass 2: Functional Impact Assessment Does it HIT a NR? Yes Assess Functional Impact No Stop Quantitative Indicator of Potential NR Impact GeneBLAzer Cell-Based Nuclear Receptor Assays Quantitative Measurement of Functional Cell Impact Scenario 2: One Pass Functional Answer GeneBLAzer Cell-Based Nuclear Receptor Assays + ToxBLAzer Substrate Functional Readout AND Cytotoxic Readout CONCURRENT Quantitative Measurement of Functional Cell Impact and Flag Toxicity Scalable, HTS compatible options for determining impact on nuclear receptors 14 11/9/2011 Life Technologies Proprietary and confidential

15 Questions? Thank you!