Stem cells from human hair follicles: first mechanical isolation for immediate autologous clinical use in androgenetic alopecia and hair loss

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1 Orignial rticle Page 1 of 10 Stem cells from human hair follicles: first mechanical isolation for immediate autologous clinical use in androgenetic alopecia and hair loss Pietro Gentile 1,2, Maria G. Scioli 3, lessandra ielli 3, ugusto Orlandi 3, Valerio Cervelli 1 1 Department of 3 Contributions: approval of manuscript: ll authors. Correspondence to: ackground: in vivo Methods: Results: Conclusions: Keywords: View this article at: Introduction Eighty percent of Caucasian men experience some degree of androgenetic alopecia (G) before age 70 (1). Stem Cell Investig 2017;4

2 Page 2 of 10 Stem Cell Investigation, 2017 of resting (telogen) hair follicles containing microscopic hairs in bald scalp (5). In addition to these intrinsic changes suggests that balding scalp either lacks an activator or has Current regulations In order to understand the sense of the current European of bioprocessing of therapeutic cells. the European Parliament (EC) and of the Council 13 Included among the advanced therapy pharmaceutical products are those used for gene and somatic cell therapy Cells and tissues are to be considered products of bioprocess ll cells that have undergone a manipulation of their ccording to reflection paper on classification of The same essential function for a cell population means that the cells when removed from their original environment in the human body are used to maintain the original function in the same anatomical or histological environment Clinical Practices (GCP) for the clinical application and Ethical Committee approval. Methods Patients 3

3 Stem Cell Investigation, 2017 Page 3 of 10 C D Figure 1 Human autologous hair follicle suspension procedure and preparation Italy) (Figure 1,). fter the extraction of the scalp tissues during punch biopsy (Figure 1C Figure 1D) collected and disaggregated under sterile conditions (vertical Figure 2,) in Figure 2C). (Figure 2D system (Figure 3,) and mechanically infiltrated into the scalp of the patients affected by G (Figure 3C,D). Human autologous hair follicle suspension protocol and injection Figure 3D

4 Page 4 of 10 Stem Cell Investigation, 2017 C D Figure 2 Figures 3C,5 Figure 5 the border of the treatment half and a second along the permanent tattoo. ssessment of hair growth and clinical evaluation of study (Figure 4Figure 4 Figure 4C after the last treatment (Figure 4D Cytospin and immunocytochemistry procedures respectively. fter cell adhesion on a glass slide by

5 Stem Cell Investigation, 2017 Page 5 of 10 C D Figure 3 Results treated scalp. ( Figure 6 Figure 6 Clinical results mean hair count and hair density increases (Figure 4D) over baseline values (Figure 4 increase in hair density for the treated area and less than control area of the scalp. Discussion

6 Page 6 of 10 Stem Cell Investigation, 2017 C D Figure 4 Figure 5

7 Stem Cell Investigation, 2017 Page 7 of 10 Figure 6 follicle from dissociated cells propagated under defined tissue culture conditions is a challenge still pending in tissue engineering (11). It is then of great interest to find different strategies aiming to regenerate or neogenerate the hair follicle under interactions during the embryonic hair generation and adult hair follicles using different strategies and approaches depending on the causes of hair loss (11). centrifugation of punch biopsy from human hair follicles the preliminary results obtained by the human follicle stem improving hair density. current advances in the different experimental strategies on those involving neogenesis of hair follicles in adult individuals using isolated cells and tissue engineering. follicles from adult cells has yet been reported. Perhaps dimensional culture conditions mimicking the structure human hair follicle neogenesis (11). in vivo system et al. in the hair follicles in vivo expressed in pluripotent cells of the developing embryo and

8 Page 8 of 10 Stem Cell Investigation, 2017 follicles are related to these pluripotent stem cells that can environmental cues. et al. cells in human skin are located in the areas highlighted by in vivo contain multipotent stem cells other than epithelial and ex vivo and in vitro for cell engineering and cell replacement therapies. that hair spheres can be generated from autologous adult based therapies. involved in this crosstalk at different hair cycle stages and process called hair follicle neogenesis. approaches have been designed to regenerate hair follicles (II) regeneration of complete hair follicles from the follicles by tissue engineering. follicle regeneration retaining the information to determine limitation different approaches and experimental models using freshly or cultured isolated cells from both dermal involved neonatal and embryonic murine cells. et al. (11) cultured hair follicle epithelial stem cells and dermal histological structures reminiscent of many different stages present in epithelial cells of the companion layer. lthough cultured cells from adult human scalp in a dermal scaffold in vivo structures that recapitulate embryonic hair development. conclusion that hair follicle neogenesis using human embryonic or neonatal cells.

9 Stem Cell Investigation, 2017 Page 9 of 10 the possibility to use human follicle stem cells obtained by Conclusions preparations has a positive therapeutic effect on male the authors recommend future study and clinical trials cknowledgements Footnote to declare. Ethical Statement: all patients. References proliferation and multipotent potential of human hair and skin tissue engineering in hair follicle regeneration. population of multipotent adult stem cells from human follicular stem cells in forming not only the follicle but stem cell activation during hair regeneration. Cell Stem differentiation of adult epithelial stem cells from human

10 Page 10 of 10 Stem Cell Investigation, 2017 Cite this article as: isolation for immediate autologous clinical use in androgenetic