Livestock Diagnostic Products for Ruminants. Kylt Professional in vitro Diagnostic Solutions.
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1 Livestock Diagnostic s for Ruminants Kylt Professional in vitro Diagnostic Solutions
2 Kylt Livestock Diagnostic s for Ruminants The Kylt products are designed for rapid and precise pathogen identification or characterisation in samples derived from livestock. Kylt products are able to support trustworthy detection of various diseases to detect health risk in livestock animals. Discover Kylt Livestock Diagnostic s for Ruminants Our Kylt diagnostic products come in different configurations. Please refer to the table below for further information on: Which technique Real-Time vs conventional PCR is used in the product Which reagents are not included in the products If additional protocol steps, such as Agarose Gel Electrophoresis are needed to compile the full method The information below applies for all articles per product category. Detailed information per individual product category is listed in the following pages. If you need more information on our Kylt products and categories or you have a specific demand for a product not listed please visit us on or contact us at info@kylt.eu. Category Kylt Real-Time PCR Detection Kits x x complete kit conventional (RT-)PCR (Primers included) Real-Time (RT-)PCR (Primers and Probes included) Kylt Real-Time PCR Detection Reagents # - x - - A Kylt PCR Detection Kits (conventional) x - x x - Kylt Real-Time RT-PCR Detection Kits x x Agarose Gel Electrophoresis required Addtional Enzyme Mix required BCD Enzyme Mixes (please refer to page 7) A BCD 2x qpcr-mix # Kylt Detection Reagents contain all controls, primers and probes for pathogen-specific detection but do not include enzyme mixes. PAGE 2 0A.06, Rev 001, May 2017 Phone + 49 (0) info@kylt.eu
3 CONTENT Virus Page Kylt BRSV 4 Kylt Bovine Coronavirus 4 Kylt Bovine Herpesvirus series 4 Kylt Influenza series 4 Kylt PI3 4 Kylt Bovine Rotavirus A 4 Bacterium Page Kylt Brucella spp. 6 Kylt Campylobacter spp. 4 Kylt Chlamydophila abortus 4 Kylt Clostridium series 4 Kylt Coxiella burnetii 6 Kylt Cryptosporidium series 4 Kylt E. coli series 5 Kylt Histophilus somni 4 Kylt Leptospira, pathogenic 6 Kylt Listeria monocytogenes 6 Kylt Mycoplasma series 4 Kylt Neospora caninum 4 Kylt Pasteurella multocida 6 Kylt Salmonella spp. 6 Other Page Kylt Host Cells 4 Kylt IC-RNA 7 Kylt Negative Control 7 Kylt DNA Extractionmixes 7 Kylt RNA / DNA Purification 7 BCD Enzyme Mixes 7 Phone + 49 (0) info@kylt.eu 0A.06, Rev 001, May 2017 PAGE 3
4 Kylt Real-Time RT-PCR Detection Kits are intended for the detection of viral RNA from the pathogens listed below. The detection kits contain all reagents and controls that are necessary for the process of Reverse Transcription (RT) followed by Real-Time PCR for a reliable and accurate detection of the pathogen. Kylt BRSV Kylt Bovine Coronavirus Bovine Respiratory Syncytial virus Bovine Coronavirus Kylt Bovine Herpesvirus 4 Bovine Herpesvirus Type 4 Kylt Influenza A Kylt IVA beta RT-qPCR Kylt Influenza Virus Type D Kylt PI3 Kylt Bovine Rotavirus A Kylt Host Cells Influenza Virus Type A (exogenous control, IC-RNA) # German market authorization FLI-B 672 Influenza Virus Type A (endogenous control, ß-Actin) # German market authorization FLI-C 024 Influenza Virus Type D Bovine Parainfluenzavirus-3 Bovine Rotavirus Type A Host cells (detection of beta-actin mrna, Control reaction kit) Real-Time # please refer to section Kylt Real-Time (RT-)PCR controls for further information on available quantification standards. Kylt Real-Time PCR Detection Kits are intended for the detection of bacterial DNA from all pathogens listed below. The detection kits contain all reagents and controls necessary for reliable and accurate detection. Kylt C. difficile toxin genes Kylt Clostridium perfringens toxin genes Kylt Cryptosporidium spp. Kylt Cryptosporidium parvum Kylt Histophilus somni Kylt Mycoplasma bovis Kylt Mycoplasma ovipneumoniae Kylt Neospora caninum Clostridium difficile toxin genes (A&B) Clostridium perfringens Major (a/b/e/i) and Minor (b2/entero/netb) Toxin Genes Cryptosporidium spp. Cryptosporidium parvum Histophilus somni Mycoplasma bovis Mycoplasma ovipneumoniae Neospora caninum Real-Time PAGE 4 0A.06, Rev 001, May 2017 Phone + 49 (0) info@kylt.eu
5 Kylt E.coli virulence factor Real-Time PCR Kits are intended for the detection of Escherichia coli and further screening for specific virulence factors mentioned below. The Real-Time PCR detection kits contain all reagents and controls that are necessary for a reliable and accurate detection. Kylt E. coli screening Kylt E. coli fima Kylt E. coli fimh Kylt E. coli F4 (K88) Kylt E. coli F5 (K99) Kylt E. coli F6 Kylt E. coli F17 Kylt E. coli F18 Kylt E. coli F41 Kylt E. coli eae Kylt E. coli EAST Kylt E. coli LT Kylt E. coli STa Kylt E. coli STb Kylt E. coli Stx1 Kylt E. coli Stx2 Kylt E. coli Stx2e E. coli screening E. coli virulence factor fima E. coli virulence factor fimh E. coli virulence factor F4 (K88) E. coli virulence factor F5 (K99) E. coli virulence factor F6 E. coli virulence factor F17 E. coli virulence factor F18 E. coli virulence factor F41 E. coli virulence factor eae E. coli virulence factor EAST E. coli virulence factor LT E. coli virulence factor STa E. coli virulence factor STb E. coli virulence factor Stx1 E. coli virulence factor Stx2 E. coli virulence factor Stx2e Real-Time Phone + 49 (0) info@kylt.eu 0A.06, Rev 001, May 2017 PAGE 5
6 Kylt Real-Time PCR Detection Reagents contain all controls, primer and probes for pathogen-specific detection. Compatible enzyme mixes are available separately, please refer to page 7. Kylt Salmonella spp. Salmonella spp Kylt Brucella spp. Kylt Campylobacter spp. Kylt Chlamydophila abortus Kylt Coxiella burnetii Kylt Leptospira, pathogenic Kylt Listeria monocytogenes Brucella spp. Campylobacter spp. Chlamydophila abortus Coxiella burnetii (Q-Fever) Leptospira, pathogenic Listeria monocytogenes Kylt Bovine Herpesvirus 1 Bovine Herpesvirus Type Real-Time Kylt PCR Detection Kits (conventional) are intended for the detection of bacterial DNA from all pathogens listed below. The detection kits contain all reagents and controls necessary for re liable and accurate detection. The final analysis of the PCR products is realized by classical gel electrophoresis (Agarose Gel Electrophoresis). Kylt Pasteurella multocida Pasteurella multocida (species-specific) Gel PAGE 6 0A.06, Rev 001, May 2017 Phone + 49 (0) info@kylt.eu
7 Kylt RNA/DNA Purification Kits are intended for the purification of high quality RNA and DNA from a vast variety of veterinary samples or from pure bacterial or viral culture isolates. Kylt DNA Extractionmix is intended for simplified and economic DNA extraction from pure bacterial isolates or bacterial enrichments only. Kylt DNA Extractionmix II Kylt DNA Extractionmix III Kylt RNA/DNA Purification Kit DNA Extraction from pure bacterial isolates or bacterial enrichments DNA Extraction as above from gram-positive bacteria (e.g. Listeria monocytogenes) Combined RNA and DNA purification from veterinary samples Article Number x BCD Enzyme Mixes are suitable to be combined with the respective Kylt Detection Reagents to complete the (Real-Time) (RT-) PCR method. These Mixes ensure outstanding performance and stability, for Real-Time as well as conventional PCR with or without Reverse Transcriptase. Article Number BCD 2x qpcr-mix 2 x concentrated PCR Mix for Real Time PCR BCD 2x PCR-Mix 2 x concentrated PCR Mix for conventional PCR BCD 2x RT-qPCR-Mix 2 x concentrated PCR Mix for Real-Time RT-PCR BCD 2x RT-PCR-Mix 2 x concentrated PCR Mix for conventional RT-PCR Kylt Real-Time (RT-)PCR Controls are available separately as needed. Article Number Kylt IC-RNA Internal Control RNA Kylt Negative Control Negative Control 1 ml Kylt Influenza A Standard Quantitative Standard for Influenza A Phone + 49 (0) info@kylt.eu 0A.06, Rev 001, May 2017 PAGE 7
8 The Kylt Real-Time (RT-)PCR products provide you with the following advantages: Identical thermal profiles for similar assays allow a combination in a single PCR-run. You can run them on virtually all Real-Time PCR thermal cyclers. They allow economic pooling of individual samples (in contrast to serology). They proved to be highly satisfactory and reliable in our daily high-throughput routine diagnostic services for samples from all over the world. They are fully validated according to applicable German FLI-guidelines, ISO etc. to meet all potential regulatory requirements by your authorities. They have received market authorisation in Germany by FLI (this currently applies to Kylt Salmonella spp., Kylt Influenza A, Kylt IVA beta RT-qPCR and Kylt MGS Triplex). The users of our Kylt Real-Time (RT-)PCR products benefit from: Direct support by your local technical and academic contact partner. Background support by our experts in Germany. A choice of different hands- on training options at AniCon s training center in Germany, at the local distributors site or at your site. Our Kylt Software simplifies the entry of sample data, PCR run, analysis and reporting of results. It is provided to our customers free of charge and may be customized to their individual equipment, QC-requirements and linkage to LIMS, if applicable. Regular updates of individual kits to assure inclusion of all emerging strains worldwide. Ongoing extension of the product catalogue with respect to upcoming diagnostic needs. For veterinary diagnostic use only. For in vitro use only. Regulatory requirements vary by country, these products may not be available in your geographic area AniCon Labor GmbH. All rights reserved. The trademark mentioned herein is the property of AniCon Labor GmbH or their respective owners. THE PURCHASE OF THIS PRODUCT GRANTS THE PURCHASER RIGHTS UNDER CERTAIN ROCHE PATENTS TO USE IT FOR PROVIDING VETERINARY IN VITRO DIAGNOSTIC TESTING. NO GENERAL PATENT OR OTHER LICENSE OF ANY KIND OTHER THAN THIS SPECIFIC RIGHT OF USE FROM PURCHASE IS GRANTED HEREBY. PAGE 8 0A.06, Rev 001, May 2017 Phone + 49 (0) info@kylt.eu
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