BioWhittaker specialty media Maximize your cell growth and productivity

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1 BioResearch BioWhittaker specialty media Maximize your cell growth and productivity BioWhittaker specialty media cell culture applications

2 BioResearch BioWhittaker specialty media Maximize your cell growth and productivity If your work leads to downstream therapeutic, diagnostic, or biopharmaceutical applications, serum-free media is the best choice initiating your research studies. BioWhittaker specialty media deliver outstanding permance, a broad selection, and consistent results, whether you need protein-free, non-animal (NAO) or chemically media. Our ISO 9000 and cgmp systems are recognized industry wide. BioWhittaker serum-free media deliver unique benefits: Consistent results Elimination of FBS lot qualification Elimination of FBS-borne mycoplasma and virus contamination Simplified downstream purification Reduced regulatory burdens Maximal yields General purpose media UltraCULTURE serum-free medium Delivers excellent growth and expansion of a broad range of adherent and suspension cells Simplify your media needs, no FBS needed PC-1 chemically, serum-free medium Superior media primary adherent cells HL-1 chemically, serum-free medium Unique media hybridoma and lymphocytic cells UltraCULTURE medium p Most cell types PC-1 CD medium p p Adherent primary cells and cell lines HL-1 CD medium p Hybridomas and many adherent cell lines UltraCULTURE medium 500 ml PC-1 CD medium 2 X 500 ml HL-1 CD medium 2 X 500 ml 2

3 Mesenchymal stem cell medium MSCGM-CD chemically, serum-free MSC growth medium multiple passage expansion of all types of hmscs Cells can be directly transitioned from serum-containing medium with little to no adaptation time No need attachment matrix to plate cells Supports multi-lineage differentiation MSCGM-CD mesenchymal stem cell chemically growth medium BulletKit Kit MSCBM-CD mesenchymal stem cell chemically basal medium 500 ml MSCGM-CD mesenchymal stem cell chemically growth medium SingleQuots kit 5 ml Fibroblast medium FGM-CD chemically, serum-free fibroblast growth medium adult normal dermal fibroblasts and also supports neonatal cells Supports expression of fibroblast markers Ab5B5, CD90, and collagen I Collagen production is superior to that obtained with DMEM + FBS No weaning seamless transition from serum-containing media FGM-CD fibroblast growth medium chemically, BulletKit with L-glutamine, without phenol red or antibiotics Kit FBM-CD fibroblast basal medium chemically 500 ml FGM-CD fibroblast growth medium chemically, SingleQuots kit Kit Cryopreservation medium ProFreeze CDM (2X), protein-free freezing medium Formulated cryopreserving all cell types Maintains high cell viability upon recovery from frozen storage Requires 15% DMSO at 2X ProFreeze CDM (2X) 100 ml 3

4 BioResearch BioWhittaker specialty media Hematopoietic media X-VIVO chemically, serum-free hematopoietic cell media Superior media most hematopoietic cell types including dendritic cells, lymphocytes, monocytes, macrophages, and granulocytes Highly cited in scientific literature X-VIVO 10 medium p PBL, LAK, monocytes, macrophages, stem cells X-VIVO 15 medium p p PBL, NK, TIL, monocytes, macrophages, stem cells, dendritic cells X-VIVO 20 medium p PBL, LAK, TIL, monocytes, macrophages, stem cells, dendritic cells X-VIVO 10 with L-glutamine, gentamicin and phenol red 1 L X-VIVO 10 with L-glutamine, without gentamicin or phenol red 1 L X-VIVO 15 with L-glutamine, without gentamicin or phenol red 1 L X-VIVO 20 with L-glutamine, gentamicin and phenol red 1 L X-VIVO 15 with L-glutamine, gentamicin and phenol red 1 L PER.C6 medium ProPer 1 chemically, serum-free medium human embryonic retinoblast PER.C6 (and related) cells Recombinant protein and virus production ProPer 1 CD medium p p p PER.C6 (and related) cells in suspension ProPer 1 CD medium 1 L 4

5 CHO media PowerCHO chemically, serum-free CHO media New chemically PowerCHO media bring new levels of cell proliferation and protein production to chemically media Maintain high viability (>90%) at high cell densities UltraCHO serum-free CHO medium The al medium serum-free CHO use with suspension or adherent cells ProCHO protein-free CHO media Multiple mulas to optimize your protein-free applications including adherent and suspension cells, with high proliferation rates, and high protein yield Directly convert cultures from adherent with serum to suspension without serum PowerCHO and ProCHO IgG production ProCHO 4 supports concurrent transition to serum-free medium IgG (ng/ml) 2,500 2,000 1,500 1, Viable Cells /ml (x10 5 ) ProCHO 4 PowerCHO Passages PowerCHO 2 PowerCHO 3 ProCHO 4 Commercial supplier A Commercial supplier A Commercial supplier B Commercial supplier B Commercial supplier A + Insulin Commercial supplier C Commercial supplier D Commercial supplier B + Insulin CHO-K1 cells grown adherently in T-flasks using DMEM/F % FBS were subcultured directly into 500 ml spinner flasks containing 200 ml of the respective medium. PowerCHO 1, 2, 3 CD media p p p Suspension CHO ProCHO 4, 5 media p p Suspension CHO ProCHO AT medium p p Adherent CHO UltraCHO medium p Adherent and suspension CHO PowerCHO 1 CD medium 1 L PowerCHO 2 CD medium 1 L PowerCHO 3 CD medium 1 L ProCHO 4 medium 1 L ProCHO 5 medium 1 L ProCHO AT medium 1 L UltraCHO medium 1 L 5

6 BioResearch BioWhittaker specialty media Renal media Pro293 chemically media Formulas both suspension and adherent culture ProVero 1 medium Supports Vero and MDCK in viral vaccine applications Pro293 s easy adaptation to suspension culture UltraMDCK chemically medium Supports MDCK in viral vaccine applications at both high and low densities Pro293 a supports adherent high-density cell growth Viable Cells /ml (x 10 5 ) 12 60, , , ,000 Viable Cells (RFU) 4 20, , Days Pro293 s-cdm Commercial supplier A Pro293 a-cdm Commercial supplier B Days Pro293 s CD medium p p p HEK 293 suspension Pro293 a CD medium p p p HEK 293 adherent ProVero 1 medium p p Vero, MDCK UltraMDCK CD medium p p MDCK, Vero Pro293 s CD medium 1 L Pro293 a CD medium 1 L ProVero 1 medium 1 L UltraMDCK CD medium 1 L 6

7 Insect medium Insect-XPRESS protein-free insect cell medium Versatile medium shaker flask or stationary culture of SF9, SF21, High Five and Drosophila cells Insect-XPRESS medium p SF9, SF21, High Five and Drosophila cells Insect-XPRESS medium 500 ml Insect-XPRESS medium 1 L Hybridoma media UltraDOMA chemically, serum-free hybridoma medium Designed cultivation of murine, human and chimeric hybridomas in batch culture ProDoma serum-free hybridoma medium, protein-free media your hybridoma needs Scalable from small flasks to large-scale bioreactors UltraDOMA -PF chemically, protein-free hybridoma medium Based on the al hybridoma media murine, human, and chimeric cells in a completely medium with no peptides or tissue extracts UltraDOMA CD medium p p Murine, human, and chimeric cell lines UltraDOMA -PF CD medium p p p p Murine, human, and chimeric cell lines ProDoma 1 CD medium p p p Murine, rat, human, and chimeric cell lines ProDoma 3 medium p p Murine, rat, human, and chimeric cell lines UltraDOMA CD medium 500 ml UltraDOMA -PF CD medium 500 ml ProDoma 1 CD medium 1 L ProDoma 3 medium 1 L 7

8 Contact inmation North America Scientific support: (toll free) Lonza Cologne GmbH Cologne, Germany For research use only. Not use in diagnostic procedures. PER.C6 and Permexcis are trademarks of Crucell N.V. High Five is a trademark of Boyce Thompson Institute Plant Research Inc. Unless otherwise noted, all trademarks herein are marks of the Lonza Group or its affiliates. The inmation contained herein is believed to be correct and corresponds to the latest state of scientific and technical knowledge. However, no warranty is made, either expressed or implied, regarding its accuracy or the results to be obtained from the use of such inmation and no warranty is expressed or implied concerning the use of these products. The buyer assumes all risks of use and/or handling. No statement is intended or should be construed as a recommendation to infringe any existing patent. Copyright 2012, Lonza Cologne GmbH. All rights reserved. BR-MxCllGrwth 09/11 CD-BR020 VWR Prices and product details are current when published; subject to change without notice. Certain products may be limited by federal, state, provincial, or local regulations. VWR makes no claims or warranties concerning sustainable/green products. Any claims concerning sustainable/green products are the sole claims of the manufacturer and not those of VWR International, LLC. All prices are in US dollars unless otherwise noted. Offers valid in US, void where prohibited by law or company policy, while supplies last. VWR, the VWR logo and variations on the egoing are registered ( ) or unregistered trademarks and service marks, of VWR International, LLC and its related companies. All other marks referenced are registered by their respective owner(s). Visit vwr.com to view our privacy policy, trademarks owners and additional disclaimers VWR M Lit. No