Cell Biology Products

Transcription

1 Cell Biology Products

2 PromoKine a brand of PromoCell Cell Biology Cell Culture Training Courses Please visit our websites regularly for upcoming new products and training courses

3 Overview 1 Overview 1 Cell Analysis 2 Apoptosis 3 Fluorescent Labeling 4 Gene Cloning, Expression, and Silencing 5 Cell Transfection 6 Immunology 7 Cytokines and Growth Factors 8 Microbial Detection and Elimination

4 2 Contents Contents Cell Analysis 1.1 Cell Viability Kits Live/Dead Cell Staining Kits Cytotoxicity Kits Senescence Detection Kit Cell-Based Reporter Assays Cell Staining Reagents and Related Products Intracellular Indicators and Related Products Cell Signaling Assays Cell Stress and Metabolism Cell Fractionation and Protein Extraction Kits Nucleic Acid Isolation Kits Apoptosis 2.1 DNA Fragmentation Detection Kits Mitochondrial Apoptosis Staining Kit Cytochrome c Apoptosis Detection Kit Glutathione Detection Kits Annexin V Detection Kits Caspase Kits Cathepsin/Calpain Detection Kits Kinase Detection Kits Apoptotic and Necrotic Cell Detection Apoptotic Cell Isolation Kit Apoptosis Inducers Fluorescent Labeling 3.1 PromoFluor Dyes PromoFluor Labeling Kits PromoFluor Biomolecules Labeling Kits PromoFluor DNA Labeling Kits PromoFluor Labeled Biomolecules PromoFluor Labeled Antibodies PromoFluor Labeled Phalloidin PromoFluor Labeled Biotin, Avidin, and Streptavidin PromoFluor Labeled Nucleotides Phycobiliprotein Dyes Phycobiliprotein Labeling Kits Phycobiliprotein Labeled Biomolecules PromoFluor Antifade Reagent For prices please see

5 Contents Gene Cloning, Expression, Silencing 4.1 Plasmid Vectors ppk-cmv Expression Vectors ppk-cmv Reporter Vectors ppk-cmv Fusion Vectors Gene Cloning and Expression Kits FastClone PCR Cloning Kits FastExpress Gene Expression Kits StarGate Cloning Systems StarGate Combinatorial Cloning System StarGate Mutagenesis System StarGate Fusion Cloning System Gene Silencing GeneOFF sirna Expression Kits GeneOFF shrna Vector Kits GeneOFF sirna Generation Kit Cell Transfection 5.1 PromoFectin Transfection Reagents Magnet Assisted Transfection (MATra) Transfection Accessories Immunology 6.1 Mono- and Polyclonal Antibodies Labeled Antibodies and Labeling Kits ELISA Kits Antigens and Cell Lysates Cytokines and Growth Factors 7.1 Cytokines, Chemokines, Growth Factors, and soluble Receptors CD Antigens Microbial Detection and Elimination 8.1 Mycoplasma Test Kits Mycoplasma Elimination Solutions Bacteria Test Kit Disinfectants Appendix Alphabetical Index International Distributors Terms and Conditions Ordering and Technical Support

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7 Cell Analysis 1 The specific analysis of cellular processes and conditions has played an increasingly vital role in cell biology. Monitoring reporter gene expression in cells, determining cell viability and proliferation, testing for cytotoxicity, and distinguishing live and dead cells are important procedures in most cell biology labs. Measuring intracellular signaling and assaying distinct cell metabolic activities have also become routine. These applications all require reliable, sophisticated, and user-friendly research tools.

8 6 Contents Chapter 1 1 Cell Analysis 1.1 Cell Viability Kits Live/Dead Cell Staining Kits Cytotoxicity Kits Senescence Detection Kit Cell-Based Reporter Assays Cell Staining Reagents and Related Products Intracellular Indicators and Related Products Cell Signaling Assays Cell Stress and Metabolism Cell Fractionation and Protein Extraction Kits Nucleic Acid Isolation Kits

9 1 Cell Analysis Cell Viability Kits Applications Cell viability and proliferation assays Growth factor/cytokine assays Optimization of cell culture conditions Cell number determination Benefits Non-radioactive method Easy and fast procedure Reliable and reproducible results Appropriate for various multiwell formats and automation PromoKine offers several types of cell viability kits: The Fluorometric Cell Viability Kits I and II; the Colorimetric Cell Viability Kits I, II, III, and IV; and the Bioluminescent Cell Viability Kits I and II. Because all of the kits are designed for use with various multiwell formats, they are also suited for automation. Viable cell Esterase Calcein-AM R: acetoxymethyl Calcein Fluorometric Cell Viability Kit I (Resazurin) The resazurin-based PromoKine Cell Viability Kit I provides a simple and rapid measurement of cell viability. Moreover, this single-step, homogenous cell pro-liferation assay is sensitive, safe, and costeffective. It only requires as few as 80 cells for reproducible results. The redox dye Resazurin (also known as Alamar Blue) detects cell viability by converting from a non-fluorescent, blue dye to the highly red fluorescent dye resorufin in response to chemical reduction of the growth medium caused by cell growth. The fluorescent or colorimetric signal generated from the assay is proportional to the number of living cells in the sample. The kit reagents are nontoxic to cells and allow kinetic monitoring. While fluorometric resorufin quantitation is more sensitive, colorimetric measurement is also possible. Fluorometric Cell Viability Kit II (Calcein) The PromoKine Fluorometric Cell Viability Kit II is intended for the reliable fluorometric determination of viable cell numbers. It utilizes the cell membranepermeable, non-fluorescent dye Calcein- AM that is hydrolyzed by esterases to the cell membrane-impermeable green-fluorescent Calcein (Fig. 1). Since esterases are only active in viable cells, the amount of Calcein-AM hydrolyzed by cellular esterases is directly proportional to the number of viable cells in culture. The kit has a detection range from less than 50 to more than 25,000 cells per well. Colorimetric Cell Viability Kits I (WST-8) and II (WST-1) The Colorimetric Cell Viability Kits I and II allow for easy and reliable colorimetric determination of viable cell numbers with excellent sensitivity and linearity. The kits utilize the tetrazolium salts WST-8 (Kit I) and WST-1 (Kit II) that are reduced to water-soluble, orange formazan dyes by dehydrogenases present in viable cells (see Fig. 2). The absorbance of the formazan dye is proportional to the number of metabolically active cells and can be measured directly from a 96-well plate without additional processing. 1 Cell Analysis Colorimetric Cell Viability Kits III (XTT) and IV (MTT) Fig. 1: Assay mechanism of Calcein-AM conversion by intracellular esterases. WST-8 slightly yellow Viable cell NADH, NADPH NAD, NADP WST-8 Formazan orange dye 1-Methoxy PMS Dehydrogenase Fig. 2: Assay mechanism of WST-8 conversion by dehydrogenases in viable cells. The PromoKine Colorimetric Cell Viability Kits III and IV have been developed for determining the number of viable cells in culture. They are based on the reduction of the tetrazolium salts XTT or MTT into colored formazan compounds by mitochondrial enzymes. Since these enzymes are inactivated shortly after cell death, this is a reliable method for the detection of viable cells. The absorbance of the formazan product can be measured directly from a 96-well plate without additional processing. In addition, PromoKine also provides high-quality tetrazolium salts for preparing custom cell assays including INT, MTT, XTT, and various WST salts.

10 8 1 Cell Analysis Bioluminescent Cell Viability Kit I (ATP) per well. It also offers applications besides cell viability testing, e.g., detect- lizes bioluminescence to measure the ADP/ATP ratio providing an innovative ing low level bacterial contamination in system for fast, simultaneous screening The Promokine Cell Viability Kit I blood, milk, urine, soil, and sludge. of cell proliferation, apoptosis, necrosis, 1 Cell Analysis employs bioluminescence to measure the ATP level as a marker for cell viability in mammalian cells. It utilizes luciferase to catalyze the ATP dependent, light- Bioluminescent Cell Viability Kit II (ADP/ATP) and growth arrest in mammalian cells. The ATP level is determined using the luciferin-luciferase reaction used in the Bioluminescent Cell Viability Kit I. The producing oxidation of luciferin. This The ADP/ATP ratio is a good indicator ADP level is measured by its conversion light formation can be detected using a for cell viability. High ATP and low ADP to ATP, which is subsequently detected luminometer or beta counter. levels are usually found in proliferating using the same luciferase-catalyzed re- The assay can be performed directly in cells. In contrast, decreased levels of ATP action. The assay works with full auto- culture plates requiring no harvesting, and increased levels of ADP are char- mation for high throughput, detects as washing, or sample preparation and can acteristic for apoptotic cells. In necrotic few as 100 mammalian cells per well, be fully automated for high throughput cells, the ADP levels are even higher, and offers highly consistent results with (10 seconds/sample). It is highly sensi- and ATP can be barely detected. The excellent correlation to other apoptosis tive, detecting mammalian cells Bioluminescent Cell Viability Kit II uti- markers (e.g., TUNEL-based or caspase assays). Product Size Catalog Number Fluorometric Cell Viability Kit I (Resazurin) Fluorometric Cell Viability Kit II (Calcein) 2,500 assays 10,000 assays 500 assays 1,000 assays PK-CA PK-CA PK-CA705-CK06* PK-CA Colorimetric Cell Viability Kit I (WST-8) 500 assays PK-CA705-CK04* Colorimetric Cell Viability Kit II (WST-1) 500 assays 2,500 assays PK-CA577-K PK-CA577-K Colorimetric Cell Viability Kit III (XTT) 1,000 assays PK-CA Colorimetric Cell Viability Kit IV (MTT) 1,000 assays PK-CA Bioluminescent Cell Viability Kit I (ATP) 200 assays 1,000 assays PK-CA577-K PK-CA577-K Bioluminescent Cell Viability Kit II (ADP/ATP) 200 assays PK-CA577-K255 1-Methoxy PMS INT MTT NTB PMS WST-1 WST-3 WST-5 WST-8 XTT 100 mg 500 mg 100 mg 500 mg 100 mg 500 mg 100 mg 500 mg 100 mg 500 mg 100 mg 500 mg 100 mg 500 mg 100 mg 500 mg 100 mg 500 mg 100 mg 500 mg PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA Resazurin, sodium salt 10 mg PK-CA * Not available in Japan, Korea, and China

11 1 Cell Analysis Live/Dead Cell Staining Kits Applications trates the cell membrane of living cells. two fluorescent dyes, Calcein-AM and Once inside, the cellular esterases pre- Ethidium homodimer III (EthD-III). Cal- Monitoring of viable and dead cells sent in living cells hydrolyze it to cell cein-am penetrates the cell membrane Cell counting by fluorescence micros- membrane-impermeable, green-fluores- of living cells. Once inside, the cellular copy Benefits cent Calcein (emission maximum at 515 nm). In contrast, Propidium Iodide (PI) can only pass through the disrupted mem- esterases present in living cells hydrolyze it to cell membrane-impermeable, greenfluorescent Calcein (emission maximum at 515 nm). 1 Cell Analysis Simultaneous monitoring of viable brane areas of dead cells where it in- In contrast, EthD-III can only pass and dead cells tercalates with the DNA of the nucleus, through the disrupted membrane areas High fluorescent intensity emitting red light at 617 nm (Fig 3). of dead cells where it intercalates with Fast and convenient procedure Since both Calcein and Propidium Iodide the DNA of the nucleus, emitting red Excitation and emission spectra are can be excited at 490 nm, a simultane- light at 620 nm. Compared with PI, compatible with common excitation ous monitoring of viable and dead cells is EthD-III is more stable and emits brighter sources and filter sets possible using a fluorescence microscope fluorescent signals. Since both Calcein Live/Dead Cell Staining Kit I (Fig. 4). At an excitation of 545 nm, the number of dead cells can be monitored. and EthD-III can be excited at 490 nm, a simultaneous monitoring of viable and dead cells is possible using a fluores- The Live/Dead Cell Staining Kit I utilizes Live/Dead Cell Staining Kit II cence microscope. At an excitation of two fluorescent dyes, Calcein-AM and 545 nm, the number of dead cells can Propidium Iodide. Calcein-AM pene- The Live/Dead Cell Staining Kit II utilizes be monitored. Calcein-AM R: acetoxymethyl Propidium Iodide Esterase Viable cell DNA intercalation red fluorescence Fig. 4: Double-staining of viable Calcein green fluorescence (green) and dead (red) cells using the PromoKine Live/Dead Cell Staining Kit I. Fig. 3: Assay mechanism of the PromoKine Live/Dead Cell Staining Kit I. Product Size Catalog Number Live/Dead Cell Staining Kit I 1,000 assays PK-CA705-CS01* Live/Dead Cell Staining Kit II 300 assays PK-CA Please see also the PromoKine Apoptotic/Necrotic/Healthy Cells Detection Kit in Chapter 2. * Not available in Japan, Korea, and China

12 10 1 Cell Analysis 1.3 Cytotoxicity Kits 1 Cell Analysis Applications Cytotoxicity assays Chemosensitivity testing Toxicity screening Benefits Non-radioactive method Highly sensitive Easy and fast procedure Compatible with common plate readers LDH Cytotoxicity Kits The colorimetric LDH Cytotoxicity Kits provide a fast, sensitive, and precise alternative to the radioactive [ 3 H]-thymidine and [ 51 Cr] release assays. They are based on the activity of lactate dehydrogenase (LDH), a stable cytoplasmic enzyme that is present in all cells. Damaged cells rapidly release this enzyme into the surrounding medium. LDH activity is determined by a coupled enzymatic reaction. It oxidizes lactate to pyruvate, triggering the conversion of the yellow tetrazolium salt INT into the water-soluble red formazan dye. The increase in the amount of formazan produced in culture medium can be analyzed spectrophotometrically and correlates directly to the increase in the number of lysed cells. While the LDH Cytotoxicity Kit I utilizes the tetrazolium salt INT, the new LDH Cytotoxicity Kit II includes the more sensitive and stable WST tetrazolium salt that is reduced to formazan. The kits can be used for many different in vitro cell systems to quantify cytotoxicity and cytolysis. AK Cytotoxicity Kit The bioluminometric AK Cytotoxicity Kit Lactate Formazan (red) LDH NAD + NADH + H + Catalyst is based on the measurement of adenylate kinase (AK), a ubiquitous protein present in all cells that is rapidly released into the culture medium upon damage to the plasma membrane. The simple one-step procedure involves two chemical reactions. The adenylate kinase release from the damaged cells catalyses the conversion of ADP to ATP. The luciferase catalyses a reaction between the ATP, oxygen, and luciferin producing light. The light can be measured using a luminometer or beta counter. This highly sensitive assay allows rapid quantitation of plasma membrane damage for evaluation of cell death or cytotoxicity events, and it is well suited for automation and high throughput. Fig. 5: Assay mechanism of the PromoKine LDH Cytotoxicity Kit. Pyruvate Tetrazolium INT (yellow) Product Size Catalog Number LDH Cytotoxicity Kit I 400 assays PK-CA577-K311 LDH Cytotoxicity Kit II 500 assays PK-CA577-K313 AK Cytotoxicity Kit 500 assays PK-CA577-K Senescence Detection Kit Applications Histochemical detection of senescent cells Cell senescence and oncology studies Benefits High sensitivity and specificity Compatible with common lab equipment Senescence is an arrested state in which the cells remain viable but are not stimulated to divide by serum or passage in culture. It is thought to be a tumor-suppressive mechanism and an underlying cause of aging. Senescent cells display an increase in size, senescence-associated expression of b-galactosidase (SA-b-Gal) activity, and altered patterns of gene expression. The PromoKine Senescence Detection Kit has been designed to histochemically detect a specific senescence marker (SA-b-Gal activity) in cultured cells and tissue sections. The specific histochemical marker is only present in senescent cells and is not found in presenescent, quiescent, or immortal cells. Product Size Catalog Number Senescence Detection Kit 250 assays PK-CA577-K320

13 1 Cell Analysis Cell-Based Reporter Assays Applications under these conditions, light emitted is determined by comparing its fluores- from the reaction is directly proportional cence with that of a GFP standard. The Monitoring and quantification of to the number of luciferase enzyme kit can detect a wide range of GFP con- reporter gene expression (Luciferase, molecules. The light emission from the centrations ( μg/ml). Further- b-galactosidase, GFP) Determination of transfection efficiencies Benefits luciferase reaction can be recorded using a luminometer. The Luciferase Reporter HTS Kits have been specifically designed for economic, high-throughput applications using high- more, a GFP quench solution is provided for determining autofluorescence levels of cell or tissue extracts. Beta-Galactosidase Kits 1 Cell Analysis density microplates. Highly sensitive and reproducible The b-galactosidase Staining Kit and the Fast and convenient protocols In addition to the Firefly luciferase sub- b-galactosidase Reporter Assay Kit have Compatible with common strate D-Luciferin, we also provide a been designed to detect and quantify plate readers wide range of coelenterazine substrates b-gal activity in cells transfected with Luciferase Reporter Assay Kits for the Renilla luciferase catalysed reaction. a plasmid carrying the frequently-used LacZ reporter gene. This gene encodes b-galactosidase, an enzyme that is very The PromoKine ready-to-use Luciferase GFP Reporter Assay Kit stable and resistant to proteolytic deg- Reporter Assays provide complete sys- radation. When expressed in transfected tems for rapid, convenient quantita- GFP (Green Fluorescent Protein) is a flu- cells, it converts a colorless substrate tive detection of luciferase activity in orescent protein originally isolated from (e.g., CPRG, X-Gal, or ONPG) into a eukaryotic cells, e.g., cells transfected the jellyfish Aequorea victoria. GFP and colored reaction product that can be with plasmids carrying the Firefly and/ its enhanced variant EGFP have been easily detected microscopically (b-galac- or Renilla luciferase reporter genes. They widely used as reporter proteins for in tosidase Staining Kit) or quantified using are optimized to provide excellent sensi- vivo monitoring of gene expression in a a spectrophotometer (b-galactosidase tivity as well as consistent light output variety of cell types and organisms. Since Reporter Assay Kit). when working with multiple samples. GFP requires no additional substrates or Firefly luciferase catalyzes the oxidative cofactors, GFP fluorescence can easily carboxylation of luciferin, a reaction be detected using a fluorescence micro- with the highest efficiency of any known scope. However, most imaging stud- bioluminescence reaction. Consequently, ies of GFP are qualitative. Quantitative this sensitive reporter is ideally suited for analyses of GFP expression levels in cells studying gene regulation and function. or tissues are more informative and have Renilla luciferase is often used in con- wider applications. The GFP Reporter junction with Firefly luciferase as a Assay Kit has been designed for use in normalizing transfection control or for a 96-well micro-plate format (Ex/Em = multiplexing transcriptional reporter as- 488 nm/507nm). Cells or tissues can be says. Both Firefly luciferase and Renilla homogenized directly in the provided Fig. 6: b-gal staining of CHO-K1 cells luciferase show maximum light output GFP assay buffer and the supernatant transfected with the ppk-cmv-r1 re- when substrates and co-factors are pres- of the lysed cells/tissues is transfered to porter vector expressing the lacz gene ent in large quantities. When assayed the microwell plates. The amount of GFP (see chapter 4). Product Size Catalog Number Luciferase Reporter Assay Kit I (Firefly) Luciferase Reporter Assay Kit II (Renilla) Luciferase Reporter Assay Kit III (Firefly & Renilla) Luciferase Reporter HTS Kit (Firefly) 150 assays 1,000 assays 150 assays 1,000 assays 100 assays 1,000 assays 4 ml (trial size) 10 ml 100 ml 10 x 100 m PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA T PK-CA PK-CA PK-CA GFP Reporter Assay Kit 100 assays PK-CA577-K815

14 12 1 Cell Analysis Product Size Catalog Number 1 Cell Analysis Beta-Galactosidase Staining Kit Beta-Galactosidase Reporter Assay Kit Firefly Luciferase Substrates D-Luciferin, Free Acid D-Luciferin, Potassium Salt D-Luciferin, Sodium Salt 100 assays 500 assays 100 assays 500 assays 10 mg 50 mg 1 g 10 mg 50 mg 1 g 10 mg 50 mg 1 g PK-CA578-G PK-CA578-G PK-CA578-G PK-CA578-G PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA DMNPE-caged D-Luciferin 5 mg PK-CA Renilla Luciferase Substrates Coelenterazine (native) 50 μg PK-CA Coelenterazine, 2-methyl 50 μg PK-CA Coelenterazine 400a (also known as 50 μg PK-CA DeepBlue C ) Coelenterazine cp 50 μg PK-CA Coelenterazine e 50 μg PK-CA Coelenterazine f 50 μg PK-CA Coelenterazine fcp 50 μg PK-CA Coelenterazine h 50 μg PK-CA Coelenterazine hcp 50 μg PK-CA Coelenterazine i 50 μg PK-CA Coelenterazine ip 50 μg PK-CA Coelenterazine n 50 μg PK-CA Coelenterazine-WS 1 mg PK-CA705-C397 Coelenterazine Sampler Kit 1 Kit PK-CA Chromogenic beta-galactosidase Substrates Green-beta-D-Gal 25 mg PK-CA Purple-beta-D-Gal 25 mg PK-CA Red-beta-D-Gal 100 mg PK-CA Rose-beta-D-Gal 100 mg PK-CA X-Gal 100 mg 1 g 5 g PK-CA PK-CA PK-CA Chromogenic beta-galactosidase Substrate Sampler Kit 1 kit (5 x 5 mg) PK-CA Please see chapter 4 for luciferase, beta-gal, and GFP reporter vectors.

15 1 Cell Analysis Cell Staining Reagents and Related Products Applications Detection of cellular structures Fluorescence microscopy/flow cytometry Benefits High quality Thoroughly tested Highly sensitive Fluorescent dyes offer extraordinary sensitivity and extended possibilities for the visualization and analysis of intracellular processes and cell structures such as the cell membrane, mitochondria, the nucleus, and the cytoskeleton. Some dyes are also suited for staining only viable or dead cells. That is an important feature for elucidating the effects of certain agents on cell viability. PromoKine provides a select choice of reagents for staining different cell organelles and structures as well as for determining processes like membrane potential changes. Some of these reagents are available as esters, which pass through the membranes of viable cells. Once inside, the ester group is hydrolyzed by cellular esterases, and the dye can accumulate in the cell. Some dyes are nonfluorescent in their ester form but fluoresce strongly when the ester group has been removed. Fig. 7: Primary human fibroblasts (NHDF, PromoCell) stained with Calcein and counterstained with DAPI. 1 Cell Analysis Product Product Description Size* Catalog Number Nucleus Stains Acridine Orange (AO) Nucleus stain (DNA/RNA) 10 ml PK-CA Solution (10 mg/ml) DAPI Nucleus stain (DNA) 10 mg PK-CA DAPI Solution Nucleus stain (DNA); aqueous solution 10 mg in 1 ml PK-CA Hoechst Nucleus stain (DNA) 100 mg PK-CA Hoechst Solution Nucleus stain (DNA); aqueous solution 10 ml PK-CA (10 mg/ml) Hoechst Nucleus stain (DNA) 100 mg PK-CA Hoechst Solution Nucleus stain (DNA); aqueous solution 10 ml PK-CA (10 mg/ml) Mitochondria Stains DASPEI 2-(4-(dimethylamino)styryl)-N-ethylpyridinium iodide; 100 mg PK-CA for staining of mitochondria in live cells Dihydrorhodamine 123 Reduced form of Rhodamine 123; mitochondria stain 10 mg PK-CA Dihydrorhodamine 123, dihydrochloride salt DiOC 6 (3) JC-1 chloride JC-1 iodide Reduced form of Rhodamine 123; mitochondria stain 10 mg PK-CA ,3 -Dihexyloxacarbocyanine, iodide; membrane potential sensitive dye Stains mitochondria in living cells in a membrane potential-dependent function Stains mitochondria in living cells in a membrane potential-dependent function 100 mg PK-CA mg PK-CA mg PK-CA MitoRed Red-fluorescent mitochondria stain 8 x 50 µg PK-CA705-R237 Nonyl Acridine Orange (NAO) Mitochondria stain; not dependent on mitochondrial membrane potential 50 mg PK-CA Rhodamine 123 Green-fluorescent mitochondria stain 50 mg PK-CA Tetrabromorhodamine 123, bromide TMRE TMRM * Other sizes may also be available on request. Green-fluorescent mitochondria stain 5 mg PK-CA Tetramethylrhodamine ethyl ester, perchlorate; membrane potential sensitive dye Tetramethylrhodamine methyl ester, perchlorate; membrane potential sensitive dye 25 mg PK-CA mg PK-CA

16 14 1 Cell Analysis Product Product Description Size* Catalog Number 1 Cell Analysis ER Stains DiOC 6 (3) 3,3 -Dihexyloxacarbocyanine, iodide; membrane potential sensitive dye 100 mg PK-CA Viable Cell Stains BCECF Viable cell stain (cytosol) 1 mg PK-CA BCECF AM Viable cell stain (cytosol); acetoxymethyl ester 10 x 100 µg 20 x 50 µg 1 mg BCECF AM Solution Viable cell stain (cytosol); acetoxymethyl ester, DMSO solution PK-CA PK-CA PK-CA mg in 1 ml PK-CA Calcein, high purity Viable cell stain (cytosol); ultra-pure 100 mg PK-CA Calcein AM Viable cell stain (cytosol); acetoxymethyl ester 1 mg 20 x 50 µg Calcein AM Solution Viable cell stain (cytosol); acetoxymethyl ester, 4 mm in anhydrous DMSO Calcein AM Solution Viable cell stain (cytosol); acetoxymethyl ester, 1 mg/ml in anhydrous DMSO PK-CA PK-CA µl PK-CA ml PK-CA CFSE; 5(6)-CFDA, SE 5-(and-6)-Carboxyfluorescein diacetate, 25 mg PK-CA succinimidyl ester; viable cell stain (cytosol) CytoRed Solution Viable cell stain (cytosol) 1 ml (1 mm) PK-CA705-C410 FDA (fluorescein diacetate) Viable cell stain (cytosol) 1 mg PK-CA705-F209 Dead Cell Stains EB Solution Dead cell stain (DNA); aqueous solution 10 ml PK-CA (10 mg/ml) Propidium Iodide (PI) Dead cell stain (DNA) 100 mg PK-CA Propidium Iodide (PI) Solution Dead cell stain (DNA); aqueous solution 10 ml (1 mg/ml) PK-CA Trypan Blue Dead cell stain; tetrasodium salt 5 g PK-CA705-T375 Trypan Blue Solution (0.4%) Dead cell stain; tetrasodium salt, 0.4% saline solution 10 ml PK-CA Cytoplasmic Membrane Staining Blue-fluorescent Cytoplasmic Membrane Staining Kit Green-fluorescent Cytoplasmic Membrane Staining Kit Orange-fluorescent Cytoplasmic Membrane Staining Kit Red-fluorescent Cytoplasmic Membrane Staining Kit Lipophilic Carbocyanine Dyes DiD (DiIC 18 [5]) DiI (or DiIC 18 (3)) Convenient and sensitive cell staining with low cytotoxicity Convenient and sensitive cell staining with low cytotoxicity Convenient and sensitive cell staining with low cytotoxicity Convenient and sensitive cell staining with low cytotoxicity 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine, perchlorate 1 ml PK-CA ml PK-CA ml PK-CA ml PK-CA mg PK-CA mg PK-CA DiI in vegetable oil Otimized DiI formulation for microinjection 0.5 ml PK-CA Dilinoleyl DiI 1,1 -Dilinoleyl-3,3,3,3 -tetramethylindocarbocyanine 5 mg PK-CA perchlorate (also called FAST DiI, a trademark of Molecular Probes, Inc.) Dilinoleyl DiO 1,1 -Dilinoleyl-3,3 -oxacarbocyanine, perchlorate (also 5 mg PK-CA called FAST DiO, a trademark of Molecular Probes, Inc.) DiO (or DiOC 18 (3)) 3,3'-Dioctadecyloxacarbocyanine, perchlorate 50 mg PK-CA DiOC 14 (3), hydroxyethanesulfonate DiR (DiIC 18 [7]) 3,3 -Ditetradecyloxacarbocyanine, hydroxyethanesulfonate 1,1'-Dioctadecyl-3,3,3',3'-tetramethylindotricarbocyanine, iodide 50 mg PK-CA mg PK-CA * Other sizes may also be available on request.

17 1 Cell Analysis 15 Product Product Description Size* Catalog Number Neuro-DiI Improved alternative to DiI 25 mg PK-CA Neuro-DiI in vegetable oil Optimized formulation for microinjection 0.2 ml PK-CA Neuro-DiO Improved alternative to DiI 25 mg PK-CA Neuro-DiO in vegetable oil Optimized formulation for microinjection 0.2 ml PK-CA Phospholipid Membrane Probes Biotin-DHPE N-(biotinoyl)-1,2-dihexadecanoyl-sn-glycero-3-10 mg PK-CA phosphoethanolamine, triethylammonium salt Biotin-X-DHPE N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, 5 mg PK-CA triethylammonium salt Fluorescein-DHPE N-(fluorescein-5-thiocarbamoyl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, 5 mg PK-CA triethylammonium salt NBD-DHPE N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, 10 mg PK-CA triethylammonium salt Rhodamine-DHPE N-(Lissamine Rhodamine B sulfonyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine, 5 mg PK-CA triethylammonium salt Texas Red-DHPE N-(Texas Red sulfonyl)-1,2-dihexadecanoyl-snglycero-3-phosphoethanolamine, 1 mg PK-CA triethylammonium salt TRITC-DHPE N-(teramethylrhodamine-6-thiocarbamoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, 1 mg PK-CA triethylam- monium salt Other Membrane Dyes AM3-25 Lipophilic styryl dye with an amine group at the 1 mg PK-CA hydrophilic head Biotin-AM3-25 AM3-25 conjugate with biotin that can be coupled to 1 mg PK-CA avidin or streptavidin DiA 4-(4-dihexadecylaminostyryl)-N-methylpyridinium iodide 50 mg PK-CA NBD C6-ceramide NBD C6-sphingomyelin Octadecyl Rhodamine B Chloride (R18) Nerve Terminal Staining Kits Nerve Terminal Green Staining Kit I Nerve Terminal Green Staining Kit II Nerve Terminal Green Staining Kit III Nerve Terminal Green Staining Kit IV Nerve Terminal Red Staining Kit I Nerve Terminal Red Staining Kit II 6-((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino) hexanoyl)sphingosine 1 mg PK-CA ((N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino) 1 mg PK-CA hexanoyl)sphingosyl phosphocholine Useful for membrane fusion assays 10 mg PK-CA Kit contains 5 x 1 mg SynapseGreen C4 and 250 mg 1 set PK-CA ADVASEP-7 Kit contains 1 mg AM1-43 and 100 mg ADVASEP-7 1 set PK-CA Kit contains 1 mg AM1-43 and 100 mg SCAS 1 set PK-CA Kit contains 5 x 1 mg SynapseGreen C4 and 100 mg Sulforhodamine 101 Kit contains 5 x 1 mg SynapseRed C2 and 250 mg ADVASEP-7 Kit contains 5 x 1 mg SynapseRed C2 and 100 mg Sulforhodamine set PK-CA set PK-CA set PK-CA Nerve Terminal Probes & Related Reagents ADVASEP-7 For background fluorescence reduction 250 mg PK-CA (sulfobutyl b-cyclodextrin) Alpha-Bungarotoxin (from Bungarus multicinctus) Acetylcholine receptor inhibitor 1 mg 5 mg PK-CA PK-CA AM1-43 Fixable, activity-dependent nerve terminal probe 1 mg PK-CA AM1-44 Nerve Terminal Probe 1 mg PK-CA AM2-10 Nerve Terminal Probe 1 mg PK-CA Cell Analysis * Other sizes may also be available on request.

18 16 1 Cell Analysis Product Product Description Size* Catalog Number 1 Cell Analysis AM3-25 Nerve Terminal Probe 1 mg PK-CA AM4-64 Nerve Terminal Probe 1 mg PK-CA AM4-65 Nerve Terminal Probe 1 mg PK-CA AM4-66 Nerve Terminal Probe 1 mg PK-CA Biotin-XX-a-bungarotoxin Fluorescein-a-bungarotoxin SCAS Can be used to isolate and/or image distribution of the nicotinic acetylcholine receptor at the neuromuscular junctions Can be used to image distribution of the nicotinic acetylcholine receptor at the neuromuscular junctions 4-sulfonato calix[8]arene, sodium salt; for background fluorescence reduction 500 µg PK-CA µg 10 x 50 µg Also known as Texas Red-a-bungarotoxin 500 µg 10 x 50 µg SynapseGreen C1 Nerve Terminal Probe 5 mg 5 x 1 mg SynapseGreen C18 (FM3-25) SynapseGreen C2 (FM2-10) Nerve Terminal Probe (also known as FM3-25, a trademark of Molecular Probes, Inc.) Nerve Terminal Probe (also known as FM2-10, a trademark of Molecular Probes, Inc.) PK-CA PK-CA mg PK-CA mg 5 x 1 mg 5 mg 5 x 1 mg SynapseGreen C3 Nerve Terminal Probe 5 mg 5 x 1 mg SynapseGreen C4 (FM1-43) SynapseGreen C5 (FM1-84) Nerve Terminal Probe (also known as FM1-43, a trademark of Molecular Probes, Inc.) Nerve Terminal Probe (also known as FM1-84, a trademark of Molecular Probes, Inc.) 5 mg 5 x 1 mg 5 mg 5 x 1 mg SynapseRed C1 Nerve Terminal Probe 5 mg 5 x 1 mg SynapseRed C2 (FM4-64) SynapseRed C2M (FM5-95) Sulforhodamine 101-abungarotoxin Tetramethylrhodaminea-bungarotoxin Tetrodotoxin, citrate-free Tetrodotoxin, with citrate Nerve Terminal Probe (also known as FM4-64, a trademark of Molecular Probes, Inc.) Nerve Terminal Probe (also known as FM5-95, a trademark of Molecular Probes, Inc.) Can be used to image distribution of the nicotinic acetylcholine receptor at the neuromuscular junctions Blocks the excitable sodium channels and can be used for the studies of excitable membranes of nerve, skeletal, and cardiac cells Blocks the excitable sodium channels and can be used for the studies of excitable membranes of nerve, skeletal, and cardiac cells 5 mg 5 x 1 mg 5 mg 5 x 1 mg 500 µg 10 x 50 µg 1 mg 10 x 100 µg PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA mg PK-CA PK-CA PK-CA Membrane Potential Sensitive Dyes Di-12-ANEPPQ Fast-responding membrane potential dye 5 mg PK-CA Di-2-ANEPEQ (JPW1114) Fast-responding membrane potential dye (JPW1114) 5 mg PK-CA Di-4-ANEPPS Fast-responding membrane potential dye 5 mg PK-CA Di-8-ANEPPQ Fast-responding membrane potential dye 5 mg PK-CA Di-8-ANEPPS Fast-responding membrane potential dye 5 mg PK-CA DiBAC 4 (3) Bis-(1,3-dibarbituric acid)-trimethine oxanol; 25 mg PK-CA membrane potential sensitive dye DiIC 1 (5) 1,1 -Dimethyl-3,3,3,3 -tetramethylindodicarbocyanine, 100 mg PK-CA iodide DiOC 2 (3) 3,3 -Diethyloxacarbocyanine, iodide; 100 mg PK-CA membrane potential sensitive dye DiOC 5 (3) 3,3 -Dipentyloxacarbocyanine, iodide; membrane potential sensitive dye 100 mg PK-CA * Other sizes may also be available on request.

19 1 Cell Analysis 17 Product Product Description Size* Catalog Number DiOC 6 (3) 3,3 -Dihexyloxacarbocyanine, iodide; 100 mg PK-CA membrane potential sensitive dye RH237 Fast-responding potentiometric probe 5 mg PK-CA RH414 Fast-responding potentiometric probe 5 mg PK-CA RH421 Fast-responding membrane potential dye 25 mg PK-CA RH795 Fast-responding potentiometric probe 5 mg PK-CA TMRE Tetramethylrhodamine ethyl ester, perchlorate; 25 mg PK-CA membrane potential sensitive dye TMRM Tetramethylrhodamine methyl ester, perchlorate; membrane potential sensitive dye 25 mg PK-CA Cytosolic Tracers and Related Reagents Biocytin (e-biotinoyl L-lysine) Can be introduced into cells by microinjection 100 mg PK-CA Biocytin-Hydrazide Can be introduced into cells by microinjection; aldehyde-fixable 25 mg PK-CA Biotin-Ethylenediamine, hydrochloride Biotin-Ethylenediamine, hydrobromide Functionally equivalent to Neurobiotin; 25 mg PK-CA transneuronal tracer Equivalent to Neurobiotin; transneuronal tracer 25 mg PK-CA Biotin-4-Fluorescein Can be used as polar tracer to study cell morphology 10 mg PK-CA Biotin-Rhodamine 110 Can be used as polar tracer to study cell morphology 5 mg PK-CA Calcein, high purity Viable cell stain (cytosol); ultra-pure 100 mg PK-CA Calcein AM Viable cell stain (cytosol); acetoxymethyl ester 20 x 50 µg 1 mg Calcein AM Solution, 4 mm DMSO solution Calcein AM Solution, 1 mg/ml in anhydrous DMSO PK-CA PK-CA Viable cell stain (cytosol); acetoxymethyl ester 100 µl PK-CA Viable cell stain (cytosol); acetoxymethyl ester 1 ml PK-CA DPA/Terbium Can be used for membrane and vesicle fusion assays 1 set PK-CA DPX Can be used to study membrane fusion or permeability 500 mg PK-CA Fluorescein-Biotin 5-((N-(5-(N-(6-(biotinoyl)amino)hexanoyl)amino)pentyl) 5 mg PK-CA thioureidyl) fluorescein Hydroxystilbamidine 4% in H 2 O; also known as FluoroGold TM 200 µl PK-CA Hydroxystilbamidine Also known as FluoroGold TM 10 mg PK-CA Lucifer Yellow Cadaverine Can be used as fixable fluorescent tracer 25 mg PK-CA Lucifer Yellow Cadaverine Useful tracer for studying neuronal morphology 10 mg PK-CA Biotin-X, dipotassium salt Lucifer Yellow CH lithium salt Useful polar tracer for studying neuronal morphology 25 mg PK-CA Lucifer Yellow CH potassium salt RuBPS RuBP-2S RuBP-4S Useful polar tracer for studying neuronal morphology 25 mg PK-CA Ruthenium(tris(bathophenanthrolinedisulfonate)), tetrasodium salt Ruthenium(bis(bathophenanthroline))(bathophenanthrolinedisulfonate)) Ruthenium(bathophenanthroline)(bis(bathophenanthrolinedisulfonate)), disodium salt 5 mg PK-CA mg PK-CA mg PK-CA SDIP/Europium Can be used for membrane and vesicle fusion assays 1 set PK-CA Sulforhodamine 101 Sulforhodamine B Sulforhodamine G Useful polar tracer for studying neuronal morphology and cell-cell communication Useful polar tracer for studying neuronal morphology and cell-cell communication Useful polar tracer for studying neuronal morphology and cell-cell communication 500 mg PK-CA g PK-CA g PK-CA Cell Analysis * Other sizes may also be available on request.

20 18 1 Cell Analysis Product Product Description Size* Catalog Number 1 Cell Analysis Related Products DMSO, anhydrous Dimethylsulfoxide, anhydrous 10 ml PK-CA Pluronic F-127 (sample size) Pluronic F-127, 20% solution in DMSO Pluronic F-127, 10% in H 2 O Nonionic detergent useful for solubilizing large dye molecules such as Fura-2/AM, Fluo-3/AM, Indo-1/AM and Rhod-2/AM to facilitate cell loading. Nonionic detergent useful for solubilizing large dye molecules such as Fura-2/AM, Fluo-3/AM, Indo-1/AM and Rhod-2/AM to facilitate cell loading. Nonionic detergent useful for solubilizing large dye molecules such as Fura-2/AM, Fluo-3/AM, Indo-1/AM and Rhod-2/AM to facilitate cell loading. 1 g PK-CA ml PK-CA ml PK-CA * Other sizes may also be available on request.

21 1 Cell Analysis Intracellular Indicators and Related Products Signal transduction often starts with the Derivatives for special applications Fluo-3FF, and Indo-1FF which are par- activation of a receptor on the cell sur- available ticularly useful for certain applications face which subsequently triggers a cas- Compatible with standard fluores- (e.g., spatial and functional characteri- cade of second messengers in the cell. cence equipment zation of Ca 2+ stores or detection of The change of intracellular ion concentration is a crucial step that helps second messengers transmit information. Consequently, monitoring and control of in- Calcium ions are important second messengers in cells. They are involved in muscle contraction, nerve signal trans- NMDA-induced neuronal Ca 2+ fluxes). PromoKine also provides a range of nonfluorescent calcium chelators as well as coelenterazine derivatives which are ef- 1 Cell Analysis tracellular ion concentration is a prereq- mission, immune cell activation, hor- fective luminescent calcium indicators. uisite understanding second messengers mone secretion, etc. and signal transduction in living cells. Fluorescent calcium indicators selectively bind to calcium ions and show a spec- Other Indicators Calcium Indicators tral response upon binding Ca 2+, i.e., fluorescence wavelength or intensity are Applications altered. This shift of fluorescence wave- Applications length and change of intensity upon cal- ph determination cium chelation can be monitored using Iron, zinc, and chloride ion detection Intracellular calcium signaling studies Investigation of signal transduction fluorescence microscopy. PromoKine offers a wide range of fluo- Benefits and apoptotic cascades rescent calcium indicators for detecting Neuroscience research changes in the intracellular Ca 2+ concen- High quality and sensitivity tration, including Fura-2, Fluo-3, Rhod- Benefits 2, and Indo-1 (as cell-impermeable salts In addition to our calcium probes, or cell-permeable AM esters) as well as PromoKine also offers ph indicators as High quality and sensitivity their low-affinity derivatives Fura-2FF, well as indicators for iron, zinc, and chloride ion detection. n Calcium Indicators Product Product Description Size Catalog Number Fluorescent Calcium Indicators Fluo-3 (pentaammonium salt) Fluorescent calcium indicator; Ex/Em: nm/526 nm 1 mg PK-CA Fluo-3 (pentapotassium salt) Fluorescent calcium indicator; Ex/Em: nm/526 nm 1 mg PK-CA Fluo-3 (pentasodium salt) Fluorescent calcium indicator; Ex/Em: nm/526 nm 1 mg PK-CA Fluo-3 AM Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg 20 x 50 µg 1 mg Fluo-3 AM Solution Fluorescent calcium indicator (acetoxymethyl ester; 1 mm in anhydrous DMSO) PK-CA PK-CA PK-CA ml PK-CA Fura-2 (pentaammonium salt) Fluorescent calcium indicator; Ex/Em: nm/510 nm 1 mg PK-CA Fura-2 (pentapotassium salt) Fluorescent calcium indicator; Ex/Em: nm/510 nm 1 mg PK-CA Fura-2 (pentasodium salt) Fluorescent calcium indicator; Ex/Em: nm/510 nm 1 mg PK-CA Fura-2 AM Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg 20 x 50 µg 1 mg Fura-2 AM Solution Fluorescent calcium indicator (acetoxymethyl ester; 1 mm in anhydrous DMSO) PK-CA PK-CA PK-CA ml PK-CA Indo-1 (pentaammonium salt) Fluorescent calcium indicator; Ex/Em: 330 nm/ nm 1 mg PK-CA Indo-1 (pentapotassium salt) Fluorescent calcium indicator; Ex/Em: 330 nm/ nm 1 mg PK-CA Indo-1 (pentasodium salt) Fluorescent calcium indicator; Ex/Em: 330 nm/ nm 1 mg PK-CA Indo-1 AM Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg 20 x 50 µg 1 mg PK-CA PK-CA PK-CA Quin-2 (K + salt) Fluorescent calcium indicator; Ex/Em: 339 nm/ nm 100 mg PK-CA705-Q001

22 20 1 Cell Analysis Product Product Description Size Catalog Number 1 Cell Analysis Rhod-2 (triammonium salt) Fluorescent calcium indicator; Ex/Em: 339 nm/ nm 1 mg PK-CA Rhod-2 (tripotassium salt) Fluorescent calcium indicator; Ex/Em: 339 nm/ nm 1 mg PK-CA Rhod-2 (trisodium salt) Fluorescent calcium indicator; Ex/Em: 339 nm/ nm 1 mg PK-CA Rhod-2 AM Fluorescent calcium indicator (acetoxymethyl ester) 10 x 100 µg 1 mg Fluorescent High Calcium Indicators 5,5'-difluoro-Bapta (tetrapotassium salt) PK-CA PK-CA Fluorescent calcium indicator 100 mg PK-CA ,5'-difluoro-Bapta AM Fluorescent calcium indicator (acetoxymethyl ester) 25 mg PK-CA Bis-Fura-2 Fluorescent calcium indicator 1 mg PK-CA (hexapotassium salt) Fura-2FF (K + salt) Difluorinated derivative of Fura μg PK-CA578-F1046 Fura-2FF AM Difluorinated derivative of Fura-2 (acetoxymethyl ester) 500 μg PK-CA578-F1044 Furaptra (tetrapotassium salt) Furaptra (tetrasodium salt) Furaptra, AM Fluorescent calcium indicator (also known as Mag-Fura-2) Fluorescent calcium indicator (also known as Mag-Fura-2) Fluorescent calcium indicator (also known as Mag-Fura-2) 10 x 100 µg 20 x 50 µg 1 mg 1 mg PK-CA mg PK-CA PK-CA PK-CA PK-CA Indo-1FF (K + salt) Difluorinated derivative of Indo μg PK-CA578-I1086 Indo-1FF AM Difluorinated derivative of Indo-1 (acetoxymethyl ester) 500 μg PK-CA578-I1084 Fluorescent Near-Membrane Calcium Indicators FFP-18 AM Fluorescent near-membrane calcium indicator 500 μg PK-CA578-F1052 (Fura-derivative; acetoxymethyl ester) FIP-18 AM Fluorescent near-membrane calcium indicator 500 ug PK-CA578-F1054 (Indo-derivative; acetoxymethyl ester)) Calcium Chelators Bapta (tetracesium salt) Non-fluorescent calcium chelator 1 g PK-CA Bapta (tetrapotassium salt) Non-fluorescent calcium chelator 1 g PK-CA Bapta (tetrasodium salt) Non-fluorescent calcium chelator 1 g PK-CA Bapta AM Non-fluorescent calcium chelator (acetoxymethyl ester) 25 mg 20 x 1 mg PK-CA PK-CA Bapta-FF (free acid) Difluorinated derivative of BAPTA 10 mg PK-CA578-B1091 Bapta-FF AM Difluorinated derivative of BAPTA (acetoxymethyl ester) 10 mg PK-CA578-B1093 5,5'-dimethyl-BAPTA AM Calcium chelator (acetoxymethyl ester) 25 mg PK-CA ,5'-dimethyl-BAPTA Calcium chelator 100 mg PK-CA (tetrapotassium salt) 5,5'-dibromo-Bapta Calcium chelator 100 mg PK-CA (tetrapotassium salt) 5-methyl-5'-nitro-BAPTA Calcium chelator 10 mg PK-CA (tetrapotassium salt) 5'-mononitro-BAPTA Calcium chelator 10 mg PK-CA (tetrapotassium salt) Luminescent Calcium Indicators Coelenterazine, 2-methyl Coelenterazine derivative; luminescent calcium indicator 50 μg PK-CA & luciferase substrate Coelenterazine ip Coelenterazine derivative; luminescent calcium indicator 50 μg PK-CA & luciferase substrate Coelenterazine n Coelenterazine derivative; luminescent calcium indicator & luciferase substrate 50 μg PK-CA

23 1 Cell Analysis 21 Product Product Description Size Catalog Number Coelenterazine cp Coelenterazine derivative; luminescent calcium indicator 50 μg PK-CA & luciferase substrate Coelenterazine f Coelenterazine derivative; luminescent calcium indicator 50 μg PK-CA & luciferase substrate Coelenterazine fcp Coelenterazine derivative; luminescent calcium indicator 50 μg PK-CA & luciferase substrate Coelenterazine hcp Coelenterazine derivative; luminescent calcium indicator 50 μg PK-CA & luciferase substrate Coelenterazine i Coelenterazine derivative; luminescent calcium indicator 50 μg PK-CA & luciferase substrate Coelenterazine e Coelenterazine derivative; luminescent calcium indicator 50 μg PK-CA & luciferase substrate Coelenterazine h Coelenterazine derivative; luminescent calcium indicator 50 μg PK-CA & luciferase substrate Coelenterazine-WS Beta-Cyclodextrin complex of coelenterazin; luminescent 1 mg PK-CA705-C397 calcium indicator Coelenterazine (native) Luminescent calcium indicator & luciferase substrate 50 μg PK-CA Coelenterazine 400a Coelenterazine derivative; luminescent calcium indicator 50 μg PK-CA & luciferase substrate (also known as DeepBlue C, a trademark of Perkin Elmer) Coelenterazine Sampler Kit Coelenterazine and derivatives thereof 1 Kit PK-CA Cell Analysis n Other Indicators Product Product Description Size Catalog Number Zn Indicators Calcein Blue Fluorescent Fe and Zn ion indicator 1 g PK-CA705-C002 Dansylaminoethyl-cyclen Fluorescent Zn indicator 5 mg PK-CA705-D480 Zinquin ethyl ester Blue-fluorescent Zn indicator 5 mg PK-CA Zinquin free acid Blue-fluorescent Zn indicator 5 mg PK-CA Cl Indicators MQAE N-(ethoxycarbonylmethyl)-6-methoxyquinolinium 100 mg PK-CA bromide; fluorescent chloride ion indicator SPQ 6-Methoxy-N-(3-sulfopropyl)quinolinium; 50 mg PK-CA fluorescent chloride ion indicator Heavy Metal Ion Indicators TPEN Tetrakis-(2-pyridylmethyl)ethylenediamine; heavy metal 100 mg PK-CA chelator ph Indicators 5(6)-Carboxy-2',7'- Fluorescent ph indicator 200 mg PK-CA dichlorofluorescein 5(6)-Carboxy-2',7'- Fluorescent ph indicator; cell membrane-permeant 100 mg PK-CA dichlorofluorescein diacetate 5(6)-Carboxy-2',7'- Fluorescent ph indicator; cell membrane-permeant 25 mg PK-CA dichlorofluorescein diacetate, succinimidyl ester 5(6)-Carboxyfluorescein Fluorescent ph indicator 100 mg PK-CA (6)-CFDA 5(6)-CFDA, SE 5-(and-6)-Carboxyfluorescein diacetate; fluorescent ph indicator 5-(and-6)-Carboxyfluorescein diacetate, succinimidyl ester; fluorescent ph indicator 100 mg PK-CA mg PK-CA

24 22 1 Cell Analysis Product Product Description Size Catalog Number 1 Cell Analysis 5-Carboxy-2',7'- dichlorofluorescein 5-Carboxy-2',7'- dichlorosulfonefluorescein 5-CFDA Fluorescent ph indicator (single isomer) 100 mg PK-CA Fluorescent ph indicator (single isomer) 10 mg PK-CA Carboxyfluorescein diacetate, single isomer; fluorescent ph indicator 100 mg PK-CA FAM 5-Carboxyfluorescein; fluorescent ph indicator 100 mg PK-CA CFDA 6-Carboxyfluorescein diacetate, single isomer; 100 mg PK-CA fluorescent ph indicator 6-FAM 6-Carboxyfluorescein; fluorescent ph indicator 100 mg PK-CA BCECF 2,7 -Bis-(carboxyethyl)-5-(and-6)-carboxyfluorescein; fluorescent ph indicator BCECF AM Fluorescent ph indicator; acetoxymethyl ester 10 x 100 µg 20 x 50 µg 1 mg BCECF AM Solution Fluorescent ph indicator; acetoxymethyl ester (1 mg/ml in dry DMSO) Flubida-2 Fluorescent ph indicator; conjugate of biotin and fluorescein diacetate 1 mg PK-CA PK-CA PK-CA PK-CA ml PK-CA mg PK-CA n Related Products Product Product Description Size Catalog Number 4-Bromo A (free acid) For in situ calibrations of fluorescent calcium indicators 1 mg PK-CA A (free acid) Calcimycin, Calcium Ionophore III 1 mg PK-CA Calcium Calibration Buffer Kit For calibration of fluorescent calcium indicators 2 x 50 ml PK-CA DMSO, anhydrous Dimethylsufoxide, anhydrous 10 ml PK-CA EDC (EDAC) 1-(3-Dimethylaminopropyl-3-ethylcarbodiimide, 100 mg PK-CA hydrochloride) Flubi-2 Calibration standard for Flubida-2 5 mg PK-CA Ionomycin (calcium salt) Ionomycin (free acid) Pluronic F-127 (sample size) Pluronic F-127, 20% solution in DMSO Pluronic F-127, 10% in H 2 O Useful in cell activation experiments when calcium dose-response data are not required (>90% pure) Useful for studies of Ca 2+ transport across biological membranes and measurement of cytoplasmic free Ca 2+ ; induces apoptotic neuronal degeneration in embryonic cortical neurons (>90% pure) Nonionic detergent useful for solubilizing large dye molecules such as Fura-2/AM, Fluo-3/AM, Indo-1/AM and Rhod-2/AM to facilitate cell loading Nonionic detergent useful for solubilizing large dye molecules such as Fura-2/AM, Fluo-3/AM, Indo-1/AM and Rhod-2/AM to facilitate cell loading Nonionic detergent useful for solubilizing large dye molecules such as Fura-2/AM, Fluo-3/AM, Indo-1/AM and Rhod-2/AM to facilitate cell loading 5 mg PK-CA mg PK-CA g PK-CA ml PK-CA ml PK-CA

25 1 Cell Analysis Cell Signaling Assays Applications Guanosine 3,5-cyclic monophosphate pmol cgmp can be detected. This is (cyclic GMP, cgmp) has been shown to approximately 10 times more sensitive Quantitative determination of be present at levels typically fold than similar kits. camp/cgmp levels Cell signaling and cell growth/ differentiation studies b-secretase activity detection Alzheimer s disease drug development lower than camp in most tissues and is formed by the action of the enzyme guanylate cyclase on GTP. Some hormones (e.g., acetylcholine, insulin, and oxytocin) and chemicals (e.g., serotonin and b-secretase Fluorometric Assay Kit b-secretase (BACE) is a membranebound aspartyl protease that cleaves the 1 Cell Analysis Benefits histamine) cause an increase in cgmp levels. amyloid precursor protein and is consequently a target for anti-amyloid therapy in the treatment of Alzheimer s disease. Fast and simple assays The PromoKine camp and cgmp com- Finding inhibitors of b-secretase is one Highly sensitive petitive immunoassays have been devel- of the major goals of Alzheimer s dis- camp and cgmp Immunoassay Kits oped for the quantitative determination of camp and cgmp levels. They utilize ease drug development. The PromoKine b-secretase Fluorometric Assay provides polyclonal capture antibodies against a convenient, non-radioactive system for Adenosine 3,5 -cyclic monophosphate camp or cgmp coated on a 96-well detecting b-secretase activity in biologi- (cyclic AMP, camp) is one of the most plate and HRP-conjugated camp or cal samples. The kit provides active b- important second messengers involved cgmp-hrp which competes with the secretase as positive control, b-secretase as a modulator in physiological process- camp or cgmp from the sample. After inhibitor as negative control, optimized es. It regulates neuronal, glandular, and HRP-substrate addition, the amount of peptide substrate (conjugated to two cardiovascular processes as well as im- camp-hrp or cgmp-hrp bound to the reporter molecules), and buffers for mune mechanisms, the nervous system, plate can easily be determined spectro- convenient measurement of b-secretase cell growth, and cell differentiation. A photometrically at 450 nm. The intensity activity in mammalian samples. number of hormones (e.g. ACTH, TSH, of the blue color is inversely proportional b-secretase inhibitors are also available LH) are known to activate camp via the to the camp or cgmp concentration in separately. action of the enzyme adenylate cyclase the sample. Due to a novel acetylation which converts ATP to camp. procedure fmol camp or Product Size Catalog Number camp Immunoassay Kit 100 assays PK-CA577-K371 cgmp Immunoassay Kit 100 assays PK-CA577-K372 Beta-Secretase Fluorometric Assay Kit 100 assays PK-CA577-K360 Beta-Secretase Inhibitor I 1 mg PK-CA Beta-Secretase Inhibitor II 1 mg PK-CA

26 24 1 Cell Analysis 1.9 Cell Stress and Metabolism 1 Cell Analysis Applications GST activity measurement and quantitation of GST-tagged fusion proteins SOD assays Determination of the concentration of NO metabolites (nitrate/nitrite) HAT activity measurement Metabolic studies and analysis of mutagenic, carcinogenic, and toxic effects DNA damage detection and quantification Analysis of the cell redox state Benefits Highly sensitive and reproducible detection/measurement Ready-to-use, convenient assays Fluorometric and Colorimetric GST Assay Kits Glutathione (GSH) is the major intracellular, low-molecular-weight thiol and plays a critical role in cellular defense against oxidative stress in tissues and cells. Glutathione S-transferase (GST) plays an essential role in detoxification of xenobiotics, and it mediates conjugation of glutathione with those compounds. The Fluorometric and Colorimetric GST Assays can detect GST activity in crude cell lysates or purified protein fractions and the kits also quantitate GST-tagged fusion proteins. They are based upon the GST-catalyzed reaction between GSH and the GST substrate CDNB (Colorimetric Kit) or MCB (Fluorometric Kit). CDNB has the broadest range of isozyme Xanthine O 2 Uric acid H 2 O 2 2O 2 Xanthine oxidase 2O 2 -. Fig. 8: Principle of the SOD Assay Kit. - O 3 S - O 3 S detectability (a-, µ-, f-, and other GST isoforms). Under certain conditions, the interaction between glutathione and CDNB is totally dependent on the presence of active GST. The detection limit is approximately 10 ng active GST/assay. The free form of MCB is almost nonfluorescent, whereas the dye fluoresces blue (Ex/Em = 380/461 nm) when reacting with glutathione. GST catalyzes the MCB-glutathione reaction, and the fluorescence levels are proportional to the amount of GST present in the reaction. Therefore, the GST level in samples can be easily detected using a fluorometer or a 96-well fluorometric plate reader. SOD Assay Kit Superoxide dismutase (SOD) is one of the most important antioxidative enzymes. The sensitive SOD Assay Kit utilizes WST-1 that produces a watersoluble formazan dye upon reduction with the superoxide anion. The rate of the reduction with a superoxide anion is linearly related to the xanthine oxidase (XOD) activity and is inhibited by SOD (Fig. 8). Therefore, the inhibition activity of SOD can be determined by a colorimetric method. Fluorometric and Colorimetric Nitric Oxide Kits Nitric oxide (NO) plays an important role in neurotransmission, vascular regulation, immune responses, and apoptosis. The Fluorometric and Colorimetric Nitric Oxide Assay Kits provide an accurate and - SO 3 N SOD O 2 + H 2 O 2 Inhibition reaction N N H N - SO 3 N N N+ N I NO 2 I Na + NO 2 WST-1 Formazan WST-1 convenient tool for total nitrate/nitrite concentration determination in a simple two-step process that can be used as a quantitative measure of NO production. The first step is the conversion of nitrate to nitrite utilizing nitrate reductase. The second step involves the addition of the fluorometric reagent DAN followed by NaOH which converts nitrite into a fluorescent compound (Fluorometric Nitric Oxide Kits) or the addition of Griess Reagent which converts nitrite into a deep purple azo compound (Colorimetric Nitric Oxide Kits). Measurement of the fluorescent compound reaction product or azo chromophore accurately determines the total nitric oxide production. The Fluorometric Assay Kit has been validated with culture media, plasma, and tissue homogenates. HAT Activity Assay Kit Histone acetyltransferases (HATs) have been implicated in playing a crucial role in various cellular functions such as gene transcription, cell differentiation, and proliferation. The colorimetric HAT Activity Assay Kit offers a convenient, non-radioactive system for rapid and sensitive spectrophotometric detection of HAT activity in mammalian samples. The detection can be continuous and is suitable for kinetic studies. DNA Damage Detection Kit Different physiological and environmental factors such as chemical induced damage and cell aging cause DNA lesions. AP sites (apurinic/apyrimidinic sites) are one of the major types of DNA lesions formed during the process of base excision and repair of oxidized, deaminated, or alkylated bases. It has been estimated that about 2x10 5 base lesions are generated per cell per day. For this reason, the level of AP sites in cells can be a good indicator of DNA lesions and repairs. The PromoKine DNA Damage Detection Kit utilizes the ARP (Aldehyde Reactive Probe) reagent that reacts specifically with the aldehyde group in the open ring form of the AP sites. After treat-

27 1 Cell Analysis 25 ing DNA containing AP sites with ARP reagents, the AP sites are tagged with NAD/NADH and NADP/NADPH Quantitation tion and the determination of the redox state of cells or tissue. biotin residues, which can be quantified The kits have been designed to specifical- using an avidin-biotin assay followed by PromoKine s NAD/NADH and NADP/ ly recognize NAD/NADH only or NADP/ colorimetric detection. The kit provides the necessary reagents for convenient determination of abasic sites in purified DNA samples in a 96-well plate format. NADPH Quantitation Kits provide a convenient tool for sensitive detection of the intracellular nucleotides NAD, NADH, NADP, and NADPH along with the de- NADPH only in an enzyme cycling reaction that significantly increases detection sensitivity. Purification of NAD/NADH or NADP/NADPH from sample mixes is not 1 Cell Analysis termination of their ratios. They can be required. used for studies of energy transforma- Product Size Catalog Number Colorimetric GST Assay Kit 100 assays PK-CA577-K263 Fluorometric GST Assay Kit 100 assays PK-CA577-K260 HAT Activity Assay Kit 100 assays PK-CA577-K332 Colorimetric Nitric Oxide Assay Kit 2 x 96 assays PK-CA577-K262 Fluorometric Nitric Oxide Assay Kit 2 x 96 assays PK-CA577-K252 SOD Assay Kit 100 assays PK-CA577-K335 DNA Damage Detection Kit 25 assays PK-CA577-K253 NAD/NADH Quantitation Kit 100 assays PK-CA577-K337 NADP/NADPH Quantitation Kit 100 assays PK-CA577-K347 Enzymes and Inhibitors HAT protein (P/CAF, active) 100 µg PK-RP GST Inhibitor-1 (Cibacron Blue F3G-A) 1 g PK-CA GST Inhibitor-2 (Ethacrynic acid) 1 g PK-CA Oxidized Glutathione (GSSG) 1 g PK-CA Reduced Glutathione (GSSG) 1 g PK-CA GST protein (active) 1 mg PK-RP Superoxide Dismutase (SOD, human) 100 µg PK-RP

28 26 1 Cell Analysis 1.10 Cell Fractionation and Protein Extraction Kits Applications Membrane Protein Extraction Kit mitochondrial fraction from the cytosol of mammalian cells in a simple and Cellular protein extraction from The Membrane Protein Extraction Kit staightforward procedure without ultra- mammalian cells provides an easy and complete protocol centrifugation and toxic chemicals. 1 Cell Analysis Integral membrane protein extraction Preparation of highly enriched cellular fractions (mitochondria, cytosol, nucleus) from mammalian for isolating integral membrane proteins efficiently from cultured mammalian cells. The kit has been designed to not only extract the total cellular membrane Cytosol/Particulate Fractionation Kit cells proteins but also to specifically purify the The Cytosol/Particulate Fractionation Benefits plasma membrane proteins. The procedure offers consistent yield and high Kit provides an easy and convenient system for rapid separation of cytosol and purity (>90%). Membrane proteins pre- particulate fractions. The separated frac- Convenient and efficient cell fraction- pared using the kit can be utilized in a tions are compatible with downstream ation and protein extraction variety of applications such as Western applications such as assaying cellular Easy protocols blotting, 2-D gels, enzyme analyses, distributions of small molecules and pro- Proteins and cell fractions can be used etc. The entire procedure takes less than teins or examining signal transduction for many downstream applications 1 hour. pathways. Mammalian Whole Cell Protein Extraction Kit Nuclear/Cytosol Fractionation Kit Complete Cell Fractionation Kit The Nuclear/Cytosol Fractionation Kit The Complete Cell Fractionation Kit is The Mammalian Whole Cell Protein Ex- provides a complete system that enables designed for serial sample preparation of traction Kit has been optimized for com- the separation of nuclear and cytoplasmic four distinct protein fractions, including plete extraction of all cellular proteins. fractions of mammalian cells and tissues cytosol, particulate, cytoskeleton, and It contains an optimized lysis buffer and with little or no cross-contamination, nuclear fractions from one sample. a protease inhibitor cocktail as well as maintaining the nuclear and cytoplasmic other reagents for convenient extraction compartments separate and intact. The prepared fractions are suitable for of mammalian proteins from tissues and cells under non-denaturing conditions. The entire procedure takes less than 20 Mitochondria/Cytosol Fractionation Kit many downstream assays such as twodimensional gel electrophoresis, protein immunoblotting, gel-shift assays, en- minutes, and the proteins can be used for zyme activity assays, reporter assays, many downstream applications such as The Mitochondria/Cytosol Fractionation and more. gel electrophoresis, Western blotting, etc. Kit allows isolation of a highly enriched Product Size Catalog Number Mammalian Whole Cell Protein Extraction Kit 500 assays PK-CA577-K269 Membrane Protein Extraction Kit 50 assays PK-CA577-K268 Nuclear/Cytosol Fractionation Kit Mitochondria/Cytosol Fractionation Kit 25 assays 100 assays 25 assays 100 assays PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K Cytosol/Particulate Fractionation Kit 50 assays PK-CA577-K267 Complete Cell Fractionation Kit 50 assays PK-CA577-K270

29 1 Cell Analysis Nucleic Acid Isolation Kits Applications and array-based experiments. of studies such as enzyme manipulation, Southern blotting, cloning, PCR analysis, DNA/RNA isolation Genomic DNA Isolation Kit II and amplifications. Various molecular genetic applications Benefits Fast, simple, and reproducible The Genomic DNA Isolation Kit II contains a non-organic and ready-to-use reagent for the isolation of genomic DNA from samples of human, animal, Total RNA Isolation Kit I The Total RNA Isolation Kit I is a complete kit with ready-to-use reagents for 1 Cell Analysis procedures plant, yeast, bacterial, and viral origin. the isolation of total RNA from samples Optimal yields There is no phenol step included. The of human, animal, plant, yeast, bacterial, Highly pure nucleic acids suitable for procedure is fast and can be completed or viral origin. It is based on disrupting many applications in minutes with DNA recovery of cells in guanidine thiocyanate/detergent Improved safety due to replacement % - permitting efficient isolation solution, followed by organic extraction of hazardous compounds of genomic DNA from a large number of and alcohol precipitation of the RNA, DNA Isolation Kit samples of small or large volumes. The isolated DNA can be used without addi- and the kit allows simultaneous processing of a large number of samples. tional purification for Southern blotting, The easy-to-use DNA Isolation Kit can dot blot hybridization, molecular cloning, Total RNA Isolation Kit II be used for extracting DNA from aga- RFLP, PCR, and other molecular biology rose gels, isolating plasmid DNA from applications. In the Total RNA Isolation Kit II, chlo- mini-preps, and for concentrating DNA The separately available Plant DNA roform is replaced by 1-Bromo-3-chlo- without ethanol precipitation. The puri- Isolation Buffer binds the reactive poly- ropropane (BCP) - thus reducing toxic fication procedures are quick and yield phenolic and polysaccharide contami- material handling without any adverse pure DNA that can be used in ligations, nants present in plant tissue. The also effects on the quality of isolated RNA, PCR, cloning, probe construction, se- separately available Blood DNA Isola- DNA, or proteins. quencing, etc. tion Buffer has been designed for the The resulting RNA is suitable for the iso- Genomic DNA Isolation Kit I isolation of genomic DNA from frozen or fresh blood. Both isolation buffers are lation of Poly A + RNA, Northern blotting, dot blotting, in vitro translation, cloning, intended for use with the PromoKine RT-PCR, RNase protection assays, and The Genomic DNA Isolation Kit I pro- Genomic DNA Isolation Kit II. other analytical procedures. vides a simple and convenient procedure for rapid isolation of genomic DNA from Mitochondrial DNA Isolation Kit The RBC Lysis Solution selectively lyses mammalian cells and tissue samples with erythrocytes and is intended for use in high yield and purity. The novel method The Mitochondrial DNA Extraction Kit the isolation of leukocytes from whole requires less than 90 minutes to prepare provides a convenient tool for isolating blood. It can be used with the kits highly pure genomic DNA that is free mtdna from a variety of cells and tis- PK-MB , PK-MB , of protein and RNA and is suitable for a sues with high yield and purity, without and PK-MB variety of applications such as PCR, DNA contamination from genomic DNA. The hybridization, cloning, Southern blotting, purified mtdna can be used for a variety Product Size Catalog Number DNA Isolation Kit isolations PK-MB Genomic DNA Isolation Kit I 50 isolations PK-MB577-K281 Genomic DNA Isolation Kit II 50 ml PK-MB Plant DNA Isolation Buffer 10 ml PK-MB Blood DNA Isolation Buffer 100 ml PK-MB Mitochondrial DNA Isolation Kit 50 isolations PK-CA577-K280 Total RNA Isolation Kit I 100 ml PK-MB Total RNA Isolation Kit II 100 ml PK-MB RBC Lysis Solution 100 ml PK-MB

30

31 Apoptosis 2 Apoptosis, or programmed cell death, is a sophisticated process that involves an intricate series of biochemical events. Specific cell death signals are transduced by different biomolecules such as caspases, which initiate an apoptotic cascade. This process culminates in multiple events such as degradation of cellular proteins and chromosomal DNA, mitochondrial disruption, and changes in plasma membrane integrity.

32 30 Contents Chapter 2 2 Apoptosis 2.1 DNA Fragmentation Detection Kits Mitochondrial Apoptosis Staining Kit Cytochrome c Apoptosis Detection Kit Glutathione Detection Kits Annexin V Detection Kits Caspase Kits Cathepsin/Calpain Detection Kits Kinase Detection Kits Apoptotic and Necrotic Cell Detection Apoptotic Cell Isolation Kit Apoptosis Inducers... 43

33 2 Apoptosis DNA Fragmentation Detection Kits Applications triphosphate nucleotides) to the frag- cell preparations. It includes positive mented DNA strands. Br-dUTP is more and negative control slides and utilizes Detecting fragmented readily incorporated into DNA strand Br-dUTP, biotin-labeled anti-brdu anti- nucleosomal DNA breaks than other larger ligands (e.g., bodies, and HRP-streptavidin conjugate. Screening for apoptotic cells fluorescein, biotin, or digoxigenin). The Immunohistochemical staining results Fluorescence microscopy incorporated Br-dUTP is subsequently can be analyzed with a microscope. Flow cytometry Benefits labeled with a green (Kit I) or a redfluorescent (Kit II) anti-brdu monoclonal antibody resulting in a very bright signal. Apoptotic DNA Ladder Detection Kit I The labeled DNA can then be observed High sensitivity by fluorescence microscopy or analyzed The Apoptotic DNA Ladder Detection Appropriate for various multiwell by flow cytometry. The DNA Fragmenta- Kit I provides an easy and sensitive formats and automation Compatible with standard lab equipment Internucleosomal DNA fragmentation is tion Kit II works also very well with GFP transfected cells. Fluorometric DNA Fragmentation Detection Kit III (F-dUTP) method for detecting DNA fragmentation in apoptotic cells. DNA preparation is done in less than 90 minutes without the need for extraction or separation columns. Afterwards, the DNA frag- 2 Apoptosis a key sign of apoptosis in mammalian ments can easily be visualized by aga- cells. PromoKine offers four different kits The Fluorometric DNA Fragmentation rose gel electrophoresis. This kit allows for analyzing DNA fragmentation which Detection Kit III is a modified TUNEL as- the efficient recovery of small DNA frag- are based on fluorometric or colorimetric say. It provides all reagents required for ments ensuring high sensitivity. assays. Since all of the kits are designed the specific detection of apoptotic cells in The Apoptotic DNA Ladder Detection for use with various multiwell formats, a cell population, in tissue sections, and Kit I plus additionally includes a highly they are also suited for automation. in fixed cell preps. The kit utilizes termi- sensitive DNA staining dye for improved Fluorometric DNA Fragmentation Detection Kits I and II (BrdU) The PromoKine DNA Fragmentation nal deoxynucleotidyl transferase (TdT) to catalyze the incorporation of fluorescein- 12-dUTP at the free 3 -hydroxyl ends of the fragmented DNA. The fluoresceinlabeled DNA can then be observed by visualization of DNA fragmentation by agarose gel electrophoresis. Apoptotic DNA Ladder Detection Kit II Detection Kits I and II are based on the fluorescence microscopy or analyzed by TUNEL method and identify DNA strand flow cytometry. Using the Apoptotic DNA Ladder Extrac- breaks that occur during apoptosis. The kits provide all required components - including positive and negative control Colorimetric DNA Fragmentation Detection Kit (IHC) tion Kit II allows the selective isolation of DNA ladders without interference of regular genomic DNA. The kit can detect cells and tissue slides - for convenient apoptotic DNA ladders from tissues and detection of DNA fragmentation by fluo- The ready-to-use, TUNEL-based Colo- cells with an apoptotic cell population rescence microscopy or flow cytometry. rimetric DNA Fragmentation Detection of as little as 5%. The kit specifically ex- The TUNEL assay employs a terminal Kit (IHC) has been specifically designed tracts laddered DNA, not genomic DNA. deoxynucleotidyl transferase (TdT) to for expedient detection of DNA frag- add Br-dUTP (bromolated deoxyuridine mentation in tissue sections and fixed Product Size Catalog Number Fluorometric DNA Fragmentation Detection Kit I (BrdU) 60 assays PK-CA577-K401 Fluorometric DNA Fragmentation Detection Kit II (BrdU) 60 assays PK-CA577-K404 Fluorometric DNA Fragmentation Detection Kit III (F-dUTP) 50 assays PK-CA577-K402 Colorimetric DNA Fragmentation Detection Kit (IHC) 50 assays PK-CA577-K403 Apoptotic DNA Ladder Detection Kit I 50 assays PK-CA577-K120 Apoptotic DNA Ladder Detection Kit I plus 50 assays PK-CA577-K130 Apoptotic DNA Ladder Detection Kit II 50 assays PK-CA577-K170

34 32 2 Apoptosis 2.2 Mitochondrial Apoptosis Staining Kit Applications Disruption of mitochondrial transmem- apoptotic cells, a shift in mitochondrial brane potential is one of the earliest transmembrane potential prevents the Screening for early apoptotic cells intracellular events that occurs upon the dye from accumulating in the mitochon- Fluorescence microscopy induction of apoptosis. The Mitochon- dria. Instead, it remains in the cytoplasm Flow cytometry drial Apoptosis Staining Kit provides a in its monomer form - fluorescing green. Benefits simple, fluorescent-based in vitro assay for detecting the mitochondrial changes The fluorescent signals can easily be detected by fluorescence microscopy or in apoptosis, and the kit distinguishes flow cytometry using common filters Excellent sensitivity due to high between healthy and apoptotic cells. and channels (FITC, rhodamine, PI). The fluorescence intensity The assay utilizes the novel cationic assay takes approximately 20 minutes, is Appropriate for various multiwell dye, MitoCapture TM that fluoresces highly sensitive, and detects apoptosis formats and automation differently in healthy versus apoptotic reliably in living cells. 2 Apoptosis Excitation and emission spectra are compatible with common excitation sources and filter sets cells. In healthy cells, MitoCapture TM accumulates and aggregates in the mitochondria - fluorescing brightly red. In MitoCapture Incubation Buffer, a component of the kit, is also available separately. Product Size Catalog Number Mitochondrial Apoptosis Staining Kit 25 assays 100 assays PK-CA577-K PK-CA577-K MitoCapture TM Incubation Buffer 200 ml PK-CA Cytochrome c Apoptosis Detection Kit Applications Screening for apoptotic cells Analysis of apoptotic signal cascades Benefits Non-radioactive method Easy and fast procedure No toxic chemicals necessary Cytochrome c has been shown to play an important role in apoptosis. The protein is located between the inner and outer mitochondrial membranes. An apoptotic stimulus triggers the release of cytochrome c into the cytosol where it binds to Apaf-1. The cytochrome c/apaf-1 complex activates caspase-9 which then activates caspase-3 and other downstream caspases of the apoptotic cascade. The PromoKine Cytochrome c Apoptosis Detection Kit provides an effective means for detecting cytochrome c translocation from mitochondria into the cytosol during apoptosis. The kit is easy to use and provides unique reagents for separating a highly enriched mitochondria fraction from the cytosol. Cytochrome c released from mitochondria into the cytosol is then detected by Western blotting using the cytochrome c antibody provided in the kit. The procedure is very simple and straightforward. No ultracentrifugation is required, and no toxic chemicals are involved. Product Size Catalog Number Cytochrome c Apoptosis Detection Kit 100 assays PK-CA577-K257

35 2 Apoptosis Glutathione Detection Kits Applications Screening for apoptotic cells Glutathione detection Quantitation of glutathione reductase activity Benefits Non-radioactive, highly sensitive method Easy and fast procedure Compatible with standard lab equipment Fluorometric Glutathione Detection Kit Diminished glutathione (GSH) levels occur at the early stages of apoptosis. The Fluorometric Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes. The assay utilizes a dye (monochlorobimane, MCB) that has a high affinity for glutathione. The unbound dye is almost nonfluorescent, whereas the dye fluoresces blue (excitation = 380 nm; emission = 461 nm) when bound to reduced or oxidized glutathione. Consequently, the decrease in glutathione level in apoptotic cells can be easily detected using a fluorometer or a 96-well fluorometric plate reader. Colorimetric Glutathione Detection Kit The Colorimetric Glutathione Detection Kit provides a convenient colorimetric method for analyzing either total glutathione or only the reduced form of glutathione using a microtiter plate reader. The assay utilizes the glutathione recycling system with DTNB and glutathione reductase. GSH is regenerated from GSSG (oxidized glutathione) by glutathione reductase and reacts again with DTNB to produce yellow 2-nitro- 5-thiobenzoic acid that can be detected spectrophotometrically at 412 nm (Fig. 1). This recycling reaction dramatically improves the sensitivity of total glutathione detection. The kit can quantify total glutathione from ng/well. For detecting lower glutathione concentrations, DTNB PromoKine s Colorimetric Glutathione Reductase Activity Kit has been designed for measuring glutathione reductase (GR) activity in biological samples. It is highly sensitive and well suited for high-throughput. The assay is based on the GR-catalysed reduction of GSSG to GSH, which reacts with 5,5 -Dithiobis(2- nitrobenzoic acid) (DTNB) to generate the yellow TNB 2-. The kit can detect mu/ml GR in various samples. 2-Nitro-5-thiobenzoic acid such as in blood samples, increasing the reaction time significantly improves the signal. The kit can also specifically detect the reduced form of glutathione (GSH) when glutathione reductase is omitted from the reaction mixture. Colorimetric Glutathione Reductase Activity Kit 2GSH GSSG Fig. 1: Principle of the Colorimetric Glutathione Detection Kit. Glutathione reductase + NADPH 2 Apoptosis Product Size Catalog Number Fluorometric Glutathione Detection Kit 100 assays PK-CA577-K251 Colorimetric Glutathione Detection Kit 100 assays PK-CA577-K261 Colorimetric Glutathione Reductase Activity Kit 200 assays PK-CA577-K761

36 34 2 Apoptosis 2.5 Annexin V Detection Kits Applications be tagged with EGFP (Enhanced Green in the provided binding buffer, apoptotic Fluorescent Protein). Since Annexin V cells show red fluorescence, necrotic cells Screening for apoptotic cells conjugates and tagged annexins retain show green fluorescence, and live cells Differentiating apoptotic and their high affinity for PS, they are ide- show little or no fluorescence. These necrotic cells ally suited as highly sensitive probes to populations can easily be distinguished Annexin detection identify early apoptotic cells and to de- by fluorescence microscopy and flow cy- Flow cytometry tect apoptosis much earlier than assays tometry using the appropriate filters and Fluorescence microscopy based on DNA fragmentation or loss of channels. membrane integrity. The stained cells Benefits can be evaluated by flow cytometry or Individual Annexin V Reagents fluorescence microscopy using common High fluorescence intensity and filter sets and flow cytometer channels. PromoKine also offers a wide range of 2 Apoptosis sensitivity Many different labels and tags available Annexin V Detection Kits Annexin V reagents separately. For easy staining of early apoptotic cells, Annexin V conjugated to FITC, PE, Cy3, Appropriate for various multiwell The Annexin V Apoptosis Detection Cy5, or biotin is available, as well as An- formats and automation Kits include an Annexin V conjugate or nexin V tagged with EGFP. The stained Excitation and emission spectra are tagged Annexin V as well as binding buf- cells can be evaluated by flow cytometry compatible with common excitation fer and in some cases Propidium Iodide or fluorescence microscopy using com- sources and filter sets (PI) for differentiating between early mon filter sets and flow cytometer chan- apoptotic and dead cells. The kits allow nels. Biotinylated Annexin V can be de- In the early stages of apoptosis, changes a one-step staining procedure that takes tected in conjunction with conventional can be observed on the cell surface. only 10 minutes. In addition, the assay dye-staining using any streptavidin- or Cells lose their phospholipid membrane can be performed directly on living cells. avidin-dye reagents such as PromoFluor- asymmetry and expose phosphatidyl- streptavidin/avidin conjugates, fluores- serine (PS) on the exterior plasma mem- Promokine Annexin V Apoptosis Detec- cein-, PE-, or APC-streptavidin as well brane. Annexin V is a Ca 2+ -dependent, tion Kit plus includes an additional dye, SY- as streptavidin/avidin conjugated to phospholipid-binding protein with high TOX green, that is impermeant to living peroxidase, alkaline phosphatase (AP), affinity for PS and is very useful for and apoptotic cells, but stains necrotic b-galactosidase, etc. identifying apoptotic cells that expose cells with intense green fluorescence PS on their surfaces to the extracellular by binding to cellular nucleic acids. Af- Please visit or environment. Annexin V may be conju- ter staining a cell population with e.g., see the PromoKine Price List to find our gated to biotin or fluorophores such as fluorescent Annexin V-Cy3 or Annexin complete range of individual Annexin V FITC and phycoerythrin (PE). It can also V-PE conjugates, and SYTOX Green dye reagents. Product Size Catalog Number Annexin V-Biotin Detection Kit Annexin V-Cy3 Detection Kit Annexin V-Cy3 Detection Kit Plus Annexin V-Cy5 Detection Kit Annexin V-EGFP Detection Kit Annexin V-FITC Detection Kit 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K

37 2 Apoptosis 35 Product Size Catalog Number Annexin V-FITC Detection Kit Plus Annexin V-PE Detection Kit Annexin V-PE Detection Kit Plus Annexin V-PE-Cy5 Detection Kit Annexin V-Biotin Reagent Annexin V-Cy3 Reagent Annexin V-Cy5 Reagent Annexin V-EGFP Reagent Annexin V-FITC Reagent Annexin V-PE Reagent Annexin V-PE-Cy5 Reagent 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 25 assays 100 assays 400 assays 100 μg 1 mg 200 assays 1,000 assays 200 assays 1,000 assays 200 assays 1,000 assays 200 assays 1000 assays 200 assays 1,000 assays 200 assays 1,000 assays PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA Annexin V Unlabeled Reagent 100 μg PK-CA X Annexin V Binding Buffer 100 ml PK-CA X Annexin V Binding Buffer 100 ml PK-CA Apoptosis

38 36 2 Apoptosis 2.6 Caspase Kits Caspases, a family of cysteine aspartic Caspase Detection Kits Caspase Staining Kits acid proteases, are important factors in the progression of apoptosis and can be Applications The fluorometric Caspase Staining Kits activated by various harmful physiological are the ideal tools for detecting activated stimuli such as cytochrome c release due Screening for apoptotic cells caspases in situ in living cells. The kits to mitochondrial membrane disruption, Flow cytometry and fluorescence utilize potent caspase inhibitors conju- DNA damage, ER stress, or inducers such microscopy gated to FITC or rhodamine as fluores- as TNF-alpha and FasL. Caspases are nor- Caspase detection cent markers. The caspase inhibitors mally present in the cell as inactive proenzymes. During apoptosis, they are acti- Benefits are cell-permeable, nontoxic, and bind specifically and irreversibly to the acti- vated by proteolysis forming a proteolytic vated caspases in apoptotic cells. With cascade that results in the cleavage of High fluorescent intensity and the FITC or rhodamine labels, activated 2 Apoptosis distinct proteins that are essential for cell viability. sensitivity Highly specific Fast and simple caspases in apoptotic cells can be directly visualized by fluorescence microscopy, analyzed by using flow cytometry, Appropriate for various multiwell or analyzed with a fluorescence plate formats and automation reader. Caspase inhibitors without FITC Excitation and emission spectra are or rhodamine labels are also included in compatible with common excitation the kits as negative controls. sources and filter sets The components of the kits (e.g. the Wash Buffer) are also available separately (please see Product Size Catalog Number Green Caspase-2 Staining Kit Green Caspase-3 Staining Kit Green Caspase-8 Staining Kit Green Caspase-9 Staining Kit Green Caspase-12 Staining Kit Green Multi-Caspase Staining Kit Red Caspase-3 Staining Kit Red Caspase-8 Staining Kit Red Caspase-9 Staining Kit Red Multi-Caspase Staining Kit 25 assays 100 assays 25 assays 100 assays 25 assays 100 assays 25 assays 100 assays 25 assays 100 assays 25 assays 100 assays 25 assays 100 assays 25 assays 100 assays 25 assays 100 assays 25 assays 100 assays PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K PK-CA577-K Caspase Staining Kit Wash Buffer 100 ml PK-CA For a complete list of active recombinant caspases, caspase substrates, inhibitors, and antibodies please visit our website or see the PromoKine Price List.

39 2 Apoptosis 37 Caspase Screening Kit (FC) maximum at 488 nm, emission maxi- trifluoromethyl coumarin). Active cas- mum at 530 nm). pases (provided in the kits) cleave the The PromoKine Caspase Screening Kit (FC) provides a convenient means for Caspase Drug Screening Kits synthetic substrates to release free AFC which can then be quantified using a detecting the activation of caspases by fluorescence plate reader. Compounds flow cytometry in living cells. The assay Inhibition of caspases has been shown to be screened can be directly added to is based on the cleavage of (aspartyl)2- to delay apoptosis, implicating a po- the reaction, and the level of caspase Rhodamine 110 (D2R), a reported sub- tential role in drug screening efforts. inhibition can then be determined. The strate for members of caspase family PromoKine provides a full line of Cas- assays are simple, straightforward, and proteases. The caspase substrate D2R is pase Drug Screening Kits for effective can be performed directly in microtiter nonfluorescent. However, upon cleav- screening of caspase inhibitors using plates. Assay conditions for each caspase age of the substrate by cellular activated fluorometric methods. The assays utilize have been optimized to obtain the maxi- caspases, the released rhodamine 110 gives rise to fluorescence that can be measured by flow cytometry (excitation peptide substrates containing consensus cleavage sequences specific for each caspase, labeled with AFC (7-amino-4- mal activity. 2 Apoptosis Product Size Catalog Number Caspase Screening Kit (FC) 25 assays 100 assays PK-CA577-K PK-CA577-K Caspase-1 Drug Screening Kit 100 assays PK-CA577-K Caspase-2 Drug Screening Kit 100 assays PK-CA577-K Caspase-3 Drug Screening Kit 100 assays PK-CA577-K Caspase-4 Drug Screening Kit 100 assays PK-CA577-K Caspase-5 Drug Screening Kit 100 assays PK-CA577-K Caspase-6 Drug Screening Kit 100 assays PK-CA577-K Caspase-7 Drug Screening Kit 100 assays PK-CA577-K Caspase-8 Drug Screening Kit 100 assays PK-CA577-K Caspase-9 Drug Screening Kit 100 assays PK-CA577-K Caspase-10 Drug Screening Kit 100 assays PK-CA577-K For a complete list of active recombinant caspases, caspase substrates, inhibitors, and antibodies please visit our website or see the PromoKine Price List.

40 38 2 Apoptosis Caspase Quantification Kits Applications Caspase Fluorometric & Colorimetric Assay Kits of caspase-3 or caspase-7 activity in apoptotic samples. Other caspases and Apoptosis screening non-specific proteases are not detected. Quantification of caspase activity Benefits With the Caspase Fluorometric & Colorimetric Assay Kits caspase activity in mammalian cells can be quantified by either Individual Caspase Reagents fluorescence or absorbance in a fast and In addition to the complete caspase Highly sensitive and specific simple procedure. The kits utilize the cas- staining and activity kits, PromoKine also Fast and convenient pase substrate (Ac-DEVD) 2 -R110 that is provides a variety of high quality, puri- Compatible with standard lab fluorogenic as well as chromogenic. It fied active recombinant caspases and equipment has an extinction coefficient that is 10 pro-caspases from human, mouse and Suited for automation times higher than that of pna - making rat, as well as caspase substrates and 2 Apoptosis Caspase Fluorometric Assay Kits The Caspase Fluorometric Assay Kits the assay extremely sensitive. Caspase Fluorometric Assay Kits (HTS) inhibitors. Optimized assay buffers and other supplementary reagents for setting up custom assays are also available separately. These reagents allow researchers are intended for convenient and sensi- to perform numerous caspase assays tive quantification of caspase activity The Caspase Fluorometric Assay Kits economically. Sets of ready-to-use cas- in cell lysates. The kits utilize synthetic (HTS) have been designed for economic pase enzymes, caspase substrates, and peptides which mimic the optimal sub- high-throughput applications. They use caspase inhibitors provide more conve- strate for distinct caspases and are la- the fluorogenic and chromogenic sub- nience in setting up individual assays to beled with 7-amino-4-trifluoromethyl strate (Ac-DEVD) 2 -R110 and include evaluate multiple caspases in the same coumarin (AFC). Activated caspases in specific caspase inhibitors as negative experiment. apoptotic cells specifically cleave their controls. PromoKine s active caspases are very synthetic substrates, resulting in the release of free AFC which is then quantified using a fluorometer. The kits are Caspase-3 and Caspase-7 Activity Detection Kits useful for studying enzyme regulation and kinetics, screening caspase inhibitors, determining target substrates, and fast, straight- forward, and adaptable as positive controls in caspase activity to 96-well plates. Caspase-3 plays a key role in the initiation assays and Western blot analyses. Caspase Colorimetric Assay Kits of cellular events during the apoptotic process. The PromoKine Caspase-3 and The caspase substrates come in readyto-use form for both fluorometric and Caspase-7 Activity Detection Kits allow colorimetric caspase assays. The Caspase Colorimetric Assay Kits are quantification of the specific activity intended for convenient and sensitive of caspase-3 and -7. Since caspase-3 Please visit or quantification of caspase activity in cell and -7 share the same target substrate see the PromoKine Price List to find the lysates. The kits utilize synthetic peptides sequence (DEVD), it is difficult to differ- complete range of PromoKine individual which mimic the optimal substrate for entiate the cleavage activity contributed caspase reagents. distinct caspases and are labeled with by these two caspases in vitro. Con- A range of caspase antibodies can be p-nitroanilide (pna). Activated caspases sequently, the assays utilize caspase-3 found in chapter 6. in apoptotic cells specifically cleave their or -7 specific antibodies to capture the synthetic substrates, resulting in the re- activated caspase-3 or -7 in cell lysate. lease of free pna, which is then quanti- The specific activity of caspase-3 or -7 fied using a spectrophotometer. The kits can then be analyzed using the common are fast, straightforward, and adaptable caspase-3/7 substrate DEVD-AFC. The to 96-well plates. assay system ensures specific detection

41 2 Apoptosis 39 Product Size Catalog Number Caspase-1 Fluorometric Assay Kit 25, 100, 200, 400 assays PK-CA577-K110-XXX* Caspase-2 Fluorometric Assay Kit 25, 100, 200, 400 assays PK-CA577-K116-XXX* Caspase-3 Fluorometric Assay Kit 25, 100, 200, 400 assays PK-CA577-K105-XXX* Caspase-4 Fluorometric Assay Kit 25, 100, 200 assays PK-CA577-K126-XXX* Caspase-5 Fluorometric Assay Kit 25, 100, 200, 400 assays PK-CA577-K122-XXX* Caspase-6 Fluorometric Assay Kit 25, 100, 200, 400 assays PK-CA577-K114-XXX* Caspase-8 Fluorometric Assay Kit 25, 100, 200, 400 assays PK-CA577-K112-XXX* Caspase-9 Fluorometric Assay Kit 25, 100, 200, 400 assays PK-CA577-K118-XXX* Caspase-10 Fluorometric Assay Kit 25, 100 assays PK-CA577-K124-XXX* Caspase-12 Fluorometric Assay Kit 25, 100 assays PK-CA577-K139-XXX* Caspase-1 Colorimetric Assay Kit 25, 100, 200, 400 assays PK-CA577-K111-XXX* Caspase-2 Colorimetric Assay Kit 25, 100, 200, 400 assays PK-CA577-K117-XXX* Caspase-3 Colorimetric Assay Kit 25, 100, 200, 400 assays PK-CA577-K106-XXX* Caspase-4 Colorimetric Assay Kit 25, 100 assays PK-CA577-K127-XXX* Caspase-5 Colorimetric Assay Kit 25, 100, 200, 400 assays PK-CA577-K123-XXX* Caspase-6 Colorimetric Assay Kit 25, 100, 200, 400 assays PK-CA577-K115-XXX* Caspase-8 Colorimetric Assay Kit 25, 100, 200, 400 assays PK-CA577-K113-XXX* Caspase-9 Colorimetric Assay Kit 25, 100, 200, 400 assays PK-CA577-K119-XXX* Caspase-10 Colorimetric Assay Kit 25, 100 assays PK-CA577-K125-XXX* Caspase-3 Fluorometric & Colorimetric Assay Kit Caspase-8 Fluorometric & Colorimetric Assay Kit Caspase-3 Fluorometric Assay Kit (HTS) Caspase-8 Fluorometric Assay Kit (HTS) 25 assays 100 assays 25 assays 100 assays 1 ml 10 ml 100 m 1 ml 10 ml 100 ml PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA PK-CA Caspase-3 Activity Detection Kit 100 assays PK-CA577-K163 Caspase-7 Activity Detection Kit 100 assays PK-CA577-K167 2 Apoptosis For a complete list of active recombinant caspases, caspase substrates, inhibitors, and antibodies please visit our website or see the PromoKine Price List. * XXX stands for the respective package size: 25, 100, 200, or 400 tests

42 40 2 Apoptosis 2.7 Cathepsin/Calpain Detection Kits Applications Cathepsin Activity Assay Kits Calpain Activity Assay Kits Screening for apoptotic cells The Cathepsin Activity Assay Kits are The activation of calpain is involved in Cathepsin and calpain detection fluorescence-based and utilize the pre- many physiological and pathological Benefits ferred substrate sequence for each cathepsin, labeled with AFC (amino-4-triflu- processes (e.g., apoptosis). The Calpain Activity Assay Kit provides a simple and oromethyl coumarin). Cell lysates and convenient means for analysing calpain Non-radioactive method other samples that contain cathepsins activity in apoptotic cells and other sam- Easy and fast procedure cleave the synthetic substrate to release ples. The assay is based on fluorometric Appropriate for various multiwell free AFC that can be easily quantified us- detection and quantitation of the cleav- formats and automation ing a fluorometer or a fluorescence plate age of a calpain substrate at 505 nm Compatible with common reader. The cathepsin assays are easy to using a fluorometer or a fluorescence 2 Apoptosis fluorometers/fluorescence plate readers perform and can be adapted to 96-well plate assays. Assay conditons have been optimized to obtain the maximal activ- plate reader. The kit contains positive and negative controls. Comparison of the fluorescence intensity from a treated Apoptosis can be mediated by mecha- ity. Active cathepsin as well as cathepsin sample with a normal control allows nisms other than the well-studied cas- inhibitors, and cathepsin antibodies (see determination of the changes in calpain pase-mediated cleavage cascade. There chapter 6) are also available separately. activity. Active calpain as well as calpain is growing knowledge that alternative inhibitors and calpain antibodies (see proteolytic enzymes such as the lyso- Please visit or see chapter 6) are also available separately. somal cathepsin proteases may initiate the PromoKine Price List to find more or propagate proapoptotic signals, but cathepsin products. Please visit or see it is currently unclear how cathepsin the PromoKine Price List to find more mediates apoptosis. calpain products. Product Size Catalog Number Cathepsin H Activity Assay Kit 100 assays PK-CA577-K145 Cathepsin S Activity Assay Kit 100 assays PK-CA577-K144 Cathepsin D Activity Assay Kit 100 assays PK-CA577-K143 Cathepsin L Activity Assay Kit 100 assays PK-CA577-K142 Cathepsin K Activity Assay Kit 100 assays PK-CA577-K141 Cathepsin B Activity Assay Kit 100 assays PK-CA577-K140 Calpain Activity Assay Kit 100 assays PK-CA577-K240 rhu Cathepsin D 5 µg PK-RP rhu Cathepsin L 5 µg 100 µg PK-RP PK-RP rhu Calpain I 100 µg PK-RP Cathepsin B & L Substrate Z-FR-R110 5 mg PK-CA Cathepsin Substrate Z-FR-AMC 25 mg PK-CA Cathepsin B&L Inhibitor Z-Phe-Phe-FMK 20 µl (10 mm) PK-CA C Calpain Inhibitor Z-LLY-FMK 20 µl (10 mm) PK-CA C

43 2 Apoptosis Kinase Detection Kits Applications large numbers of samples. The assay Akt Activity Detection Kit utilizes an N-terminal c-jun fusion pro- Screening for apoptotic cells tein bound to Glutathione-Sepharose The Akt Activity Detection Kit utilizes Kinase detection and activity beads to selectively pull down JNK an Akt-specific antibody to immunopre- screening from cell lysate. After a washing step to cipitate Akt from cell lysate. Akt-specific Benefits remove non-specifically bound proteins, the kinase reaction is carried out in the activity is then detected in a kinase reaction using recombinant GSK-3a as presence of cold ATP. c-jun phosphoryla- a substrate. Phosphorylation levels of Non-radioactive method tion is then determined by Western blot GSK-3a are determined by Western blot Easy, accurate, and fast procedure analysis using a phospho-c-jun specific analysis using a phospho-gsk-3a spe- Compatible with standard lab antibody. cific antibody included in the kit. The kit equipment The activation of protein kinases, such as JNK and Akt, plays an important role JNK Activity Detection Kit The JNK Activity Detection Kit utilizes specifically detects Akt1, Akt2, and Akt3 activities. Other kinase activities are not detected. Active JNK and Akt kinases as well 2 Apoptosis in apoptosis and other physiological and a JNK-specific antibody to immuno- as JNK/Akt substrates, activators, in- pathological processes. PromoKine pro- precipitate JNK from cell lysates. The hibitors, and control cell lysates are also vides a novel non-radioactive system for activity of the JNK is then detected in a available separately. convenient measurement of JNK and kinase reaction using recombinant c-jun Please visit or see Akt activities. as substrate. c-jun phosphorylation is the PromoKine Price List to find more JNK Activity Screening Kit determined by Western blotting using a phospho-c-jun specific antibody. The active kinases that can be used for cell analysis and apoptosis research. A kit specifically detects JNK activity; other range of kinase antibodies can be found The JNK Activity Screening Kit can kinase activities are not detected. in chapter 6. screen JNK activity quickly even with Product Size Catalog Number JNK Activity Screening Kit 40 assays PK-CA577-K430 JNK Activity Detection Kit 40 assays PK-CA577-K431 Akt Activity Detection Kit 40 assays PK-CA577-K435 Akt Inhibitor 1 mg PK-CA c-jun (1-79)-GST Fusion Protein 100 ug PK-CA GSK-3a-GST Fusion Protein 100 ug PK-CA GSK-3b, recombinant, human 100 ug PK-CA L-JNK Peptide Inhibitor I 50 µl (1 mm) PK-CA D-JNK Peptide Inhibitor I 25 µl (1 mm) PK-CA L-JNKI1 Negative Control Peptide 50 µl (1 mm) PK-CA D-JNKI1 Negative Control Peptide 25 µl (1 mm) PK-CA JNK Negative Control Cell Lysate 1 mg PK-CA JNK Activated Cell Lysate 1 mg PK-CA Akt Negative Control Cell Lysate 1 mg PK-CA Akt Activated Cell Lysate 1 mg PK-CA For a list of active recombinant kinases, kinase substrates, inhibitors, and antibodies please visit our website or see the PromoKine Price List.

44 42 2 Apoptosis 2.9 Apoptotic and Necrotic Cell Detection Applications within the same cell population by flow cytometry or fluorescence microscopy. Simultaneous detection of apoptotic, For tagging apoptotic cells, fluorescein necrotic, and healthy cells labeled Annexin V is used. Annexin V Fluorescence microscopy specifically binds to the phosphatidyl- Flow cytometry serin exposed on the outer membrane of apoptotic cells. In order to mark necrotic Benefits cells, Ethidium homodimer III (EthD-III) is utilized. This fluorescent DNA bind- Convenient ready-to-use kit ing substance can enter necrotic cells Fast one-step procedure because of the damaged plasma mem- Fig. 2: Apoptotic U937 cells stained Highly sensitive brane, but not living or apoptotic cells. with Annexin V-FITC using PromoKine s 2 Apoptosis Apoptosis and necrosis, the two major processes leading to cell death, show Once in the necrotic cell, it binds to cellular DNA and fluoresces red. Due to its significantly higher fluorescence quan- Apoptotic/Necrotic/Healthy Cells Detection Kit. several distinct differences. One char- tum yield, Ethidium homodimer III is acteristic of apoptotic cells is the trans- superior to propidium iodide or Ethidium location of phosphatidylserine from the homodimer I. inner to the outer side of the plasma membrane. This allows phagocytic cells to recognize and destroy apoptotic Apoptotic/Necrotic/Healthy Cells Detection Kit cells but can also be used for identifying apoptotic cells. Necrotic cells, on The Apoptotic/Necrotic/Healthy Cells the other hand, are characterized by a Detection Kit provides convenient quan- loss of membrane integrity. This allows tification of apoptotic (green), necrotic membrane-impermeable probes to enter (red), and healthy cells (blue) within the Fig. 3: Apoptotic/Necrotic U937 cell and stain them. same cell population by flow cytometry stained with Ethidium homodimer III Apoptotic/Necrotic Cells Detection Kit or fluorescence microscopy. In addition to the fluorescent dyes utilized in the Apoptotic/Necrotic Cells Detection Kit, (EthD-III) using PromoKine s Apoptotic/ Necrotic/Healthy Cells Detection Kit. it also contains a membrane-permeable The Apoptotic/Necrotic Cells Detection blue fluorescent DNA stain (Hoechst Kit provides convenient quantification of 33342) for quantifying healthy cells. apoptotic (green) and necrotic (red) cells Product Size Catalog Number Apoptotic/Necrotic Cells Detection Kit 50 assays PK-CA Apoptotic/Necrotic/Healthy Cells Detection Kit 50 assays PK-CA

45 2 Apoptosis Apoptotic Cell Isolation Kit Applications dead cells from cell culture or tissue MagBeads adhere to the magnet - preparations using Annexin V magnetic while normal cells stay in suspension. Isolation of apoptotic cells beads (MagBeads). Since Annexin V is a The bound apoptotic cells can then be Removal of dead cells from culture phospholipid-binding protein with high released from the MagBeads using the Benefits affinity for phosphatidylserine (PS), it binds strongly to the PS that is redistrib- Elution Buffer provided in the kit. The separated apoptotic cells and normal uted from the inner to the outer surface cells can be used in a variety of assays to Fast, simple, and efficient of the plasma membrane during apopto- study apoptotic mechanisms and path- Complete ready-to-use kit sis. Annexin V-biotin first binds to apop- ways. The kit has also been successfully totic cells. Then, biotin binds to strepta- used to remove dead cells from normal The PromoKine Apoptotic Cell Isolation vidin-magbeads, which enables the cultures. Kit provides a simple and efficient means separation of apoptotic cells from normal A re-usable magnetic separator opti- for isolating apoptotic cells or removing cells. The apoptotic cells bound to the mized for the kit is also available. 2 Apoptosis Product Size Catalog Number Apoptotic Cell Isolation Kit 30 isolations PK-CA577-K258 Magnetic Separator (re-usable) 1 PK-CA577-K Apoptosis Inducers Applications Induction of apoptotic processes Ideally suited for use with diverse apoptosis detection systems Benefits High quality Ready-to-use PromoKine offers a select choice of high quality apoptosis inducers in ready-to-use form. Some of them are also available in a convenient set. The apoptosis inducers are well-characterized and effective in disrupting mitochondrial transmembrane potential and activating caspases as well as inducing phosphatidyl-serine (PS) exposure, DNA fragmentation, and other apoptotic characteristics. All inducers are ideally suited for use with the various PromoKine apoptosis detection systems. PromoKine provides high quality growth factors, cytokines, and antibodies that are relevant to apoptosis research (See chapters 6 and 7). PromoKine also provides cell viability and cell proliferation assay kits that can be used for analyzing apoptotic processes (See Chapter 1). Product Size Catalog Number Apoptosis Inducer Set 1 kit PK-CA577-K121-5 Actinomycin D 5 mg 50 mg PK-CA MG PK-CA MG Actinomycin D (10 mm) 50 µl PK-CA Anisomycin 10 mg PK-CA Betulinic acid 25 mg PK-CA Brefeldin A 5 mg PK-CA Camptothecin 50 mg 500 mg PK-CA MG PK-CA MG

46 44 2 Apoptosis Product Size Catalog Number 2 Apoptosis Camptothecin (2 mm) 1 ml PK-CA Colchicine 1 g PK-CA Cycloheximide 1 g PK-CA G Cycloheximide (100 mm) 1 ml PK-CA Daunorubicin.HCl 10 mg PK-CA Dexamethasone 1 g 10 g PK-CA G PK-CA G Dexamethasone (10 mm) 1 ml PK-CA Doxorubicin.HCl 5 mg PK-CA Etoposide 25 mg 250 mg PK-CA MG PK-CA MG Etoposide (10 mm) 100 µl PK-CA Genistein 10 mg 100 mg PK-CA PK-CA Ionomycin (Calcium salt) 5 mg PK-CA Ionomycin (free acid) 5 mg PK-CA Mitomycin C 5 mg 10 mg 100 mg PK-CA578-M PK-CA578-M PK-CA578-M Okadaic acid 25 µg PK-CA Phorbol-12-myristate 13-acetate (PMA) 5 mg PK-CA Rapamycin 5 mg PK-CA Rosiglitazone 5 mg 100 mg Staurosporine 100 µg 1 mg PK-CA PK-CA PK-CA PK-CA Tamoxifen citrate 1 g PK-CA Thapsigargin 1 mg PK-CA Tyrphostin AG mg PK-CA Tyrphostin AG mg PK-CA

47 Fluorescent Labeling 3 The availability of new fluorophores has dramatically changed the possibilities for the sensitive detection of biomolecules and the analysis of their interactions. Improved fluorescent dyes are now enabling previously impossible studies of cellular structures and processes. PromoKine provides a wide range of highquality fluorophores for easy labeling of diverse biomolecules including proteins, antibodies, and DNA, as well as complete labeling kits and ready-to-use fluorescent conjugates.

48 46 Contents Chapter 3 3 Fluorescent Labeling 3.1 PromoFluor Dyes PromoFluor Labeling Kits PromoFluor Biomolecules Labeling Kits PromoFluor DNA Labeling Kits PromoFluor Labeled Biomolecules PromoFluor Labeled Antibodies PromoFluor Labeled Phalloidin PromoFluor Labeled Biotin, Avidin, and Streptavidin PromoFluor Labeled Nucleotides Phycobiliprotein Dyes Phycobiliprotein Labeling Kits Phycobiliprotein Labeled Biomolecules PromoFluor Antifade Reagent... 59

49 3 Fluorescent Labeling PromoFluor Dyes Applications qualified for a wide range of cell and PromoFluor Amino-Modified Labels molecular biology applications. In ad- Labeling biomolecules such as dition to the standard dyes, specifically PromoFluor Amino-Modified Labels proteins, antibodies, and nucleic acids designed PromoFluor-LSS dyes offer an are intended for the covalent labeling Fluorescence microscopy extra large Stokes shift, which makes of biomolecules containing a carboxy Flow cytometry them ideally suited for multicolor label- group, e.g., glycoproteins. The amino Fluorescent in situ hybridization ing and flow cytometry applications. group of PromoFluor Amino-Modified (FISH) Labels can be cross-coupled to carboxy Fluorescence correlation spectroscopy All PromoFluor dyes are provided groups through a mild in situ activation. (FCS) DNA and protein microarrays either as free carboxylic acids or in three reactive variants ready for covalent cou- PromoFluor Maleimides pling to biomolecules: amine-reactive Benefits PromoFluor NHS-Esters, PromoFluor Ami- PromoFluor Maleimides are intended for no-modified Labels, and thiol-reactive covalently labeling biomolecules con- Strong light absorption and high PromoFluor Maleimides. taining a thiol group (e.g. proteins). A fluorescence intensity Very photostable PromoFluor NHS-Esters neutral thiol-reactive label has little influence on the isoelectric point of a protein. Highly fluorescenct over a broad ph In effect, PromoFluor Maleimides have range (ph 4-9) Water-soluble without the need to use organic solvents Excitation and emission spectra are PromoFluor NHS-Esters (N-hydroxysuccinimidyl-esters) are intended for covalently labeling biomolecules containing a primary amino group (all peptides/pro- an advantage over amino group labels because they do not interfere with techniques like differential 2D gel electrophoresis. Since most proteins contain 3 Fluorescent Labeling compatible with common excitation teins contain at least one amino group only a few thiol functional groups, thiol- sources and filter sets at the N-terminus). Moreover, amino reactive dyes can be used to selectively groups can also be incorporated in other modify a protein or peptide containing PromoFluor dyes are powerful fluoro- biomolecules without changing their a thiol group at a specific site. Moreover, phores spanning almost the whole visible activity or functionality. The most com- these dyes are well suited for labeling and infrared light spectrum (see Table 1). mon examples are 5 -amino-modified oligonucleotides. Maleimides couple to They are superior to many other com- DNA oligomers and cdna containing thiol groups under very mild conditions monly used fluorescent dyes and well aminoallyl-du. at a ph around 7. Fig. 1: Left: Cell structures of HeLa cells visualized using primary anti-beta-tubulin antibodies and secondary PromoFluor 488-labeled antibodies (green) as well as primary anti-centromer antibodies and secondary PromoFlour 547-labeled antibodies (red). Middle: Rat cytokeratin (green) detected with primary rabbit anti-cytokeratin antibodies and secondary anti-rabbit IgGs labeled with PromoFluor 488. Actin (red) visualized using mouse anti-actin antibodies and secondary goat anti-mouse IgGs labeled with PromoFluor 520LSS. Right: Solid-state fluorescence of PromoFluor 633.

50 48 3 Fluorescent Labeling Name Absorption Maximum [nm] Emission Maximum [nm] Description Laser Excitation Source* PromoFluor Alternative to Alexa Fluor 405, Pacific Blue, Cascade Blue PromoFluor Alternative to Pacific Blue, Spectrum Aqua, and Diethylaminocoumarin PromoFluor 488LSS Dye with extra large Stokes shift. Ideally suited for multicolor labeling and FACS applications PromoFluor Alternative to Fluorescein (FITC), Alexa Fluor 488 and Cy TM 2 Blue diode laser Blue diode laser Argon 488 laser Argon 488 laser PromoFluor 488 Premium Very photostable alternative to Fluorescein (FITC), Alexa Fluor 488, Cy TM 2 Argon 488 laser 3 Fluorescent Labeling PromoFluor 500LSS Dye with extra large Stokes shift. Ideally suited for multicolor labeling and FACS applications Argon 488 laser PromoFluor Alternative to Rhodamine Green TM Argon 488 laser PromoFluor 510LSS Dye with extra large Stokes shift. Ideally suited for multicolor labeling and FACS applications Argon 514 laser PromoFluor 514LSS Dye with extra large Stokes shift. Ideally suited for multicolor labeling and FACS applications PromoFluor 520LSS Dye with extra large Stokes shift. Ideally suited for multicolor labeling and FACS applications PromoFluor Alternative to Alexa Fluor 546, Tetramethylrhodamine (TMR and TRITC) PromoFluor Alternative to Alexa Fluor 555, Cy TM 3 and Tetramethylrhodamine (TMR) Argon 514 laser Argon 514 laser Helium-Neon 543 laser Helium-Neon 543 laser PromoFluor Alternative to Texas Red Helium-Neon 594 laser PromoFluor Alternative to Texas Red Helium-Neon 594 laser PromoFluor Alternative to Alexa Fluor 633 Helium-Neon 633 laser and 635 diode laser PromoFluor Alternative to Alexa Fluor 647, Cy TM 5, and Allophycocyanin Krypton-Argon 647 laser PromoFluor Alternative to Alexa Fluor 680 and Cy TM 5.5 Helium-Neon laser PromoFluor Alternative to Alexa Fluor 680, Cy TM 5.5 Helium-Neon laser PromoFluor Alternative to Alexa Fluor 700 Far-red diode lasers PromoFluor Alternative to Alexa Fluor 750, Cy TM 7 Far-red diode lasers PromoFluor Alternative to Alexa Fluor 750 and Cy TM 7 Xenon-arc lamp or dye-pumped laser PromoFluor Near infrared dye allowing for deep tissue penetration and low background PromoFluor Near infrared dye allowing for deep tissue penetration and low background Xenon-arc lamp or dye-pumped laser Xenon-arc lamp or dye-pumped laser Table 1: List of available PromoFluor dyes (more PromoFluor dyes with other absorption and emission maxima are available on request). *PromoFluor dyes are compatible with common excitation sources including diode lasers, LEDs, tungsten lamps, xenon arc lamps, and custom made systems. Coupling protocols for proteins are available on our website:

51 3 Fluorescent Labeling 49 Excitation / Emission [%] Fig. 2: Excitation (left) and emission (right) spectra of PromoFluor 488LSS Wavelength [nm] 3 Fluorescent Labeling PromoFluor ChipPacks PromoFluor ChipPacks are pre-packed PromoFluor dyes intended to label cdna for microarray assays. The packs con- tain 20 µg aliquots of PromoFluor 555 and PromoFluor 647 NHS-Ester. These aliquots are sufficient for labeling the amount of cdna synthesized in a standard reverse transcription assay with enzymatic incorporation of aminoallyldutp. Customized PromoFluor Chip- Packs with other PromoFlour dyes are available on request. Product Size* Catalog Number** PromoFluor Carboxylic Acid PromoFluor NHS-Ester PromoFluor Amino-Modified Label PromoFluor Maleimide PromoFluor ChipPack 1 mg 5 mg 1 mg 5 mg 1 mg 5 mg 0.5 mg 1 mg 5 mg 20 labeling reactions 50 labeling reactions 100 labeling reactions PK-PFXXX-0-01 PK-PFXXX-0-05 PK-PFXXX-1-01 PK-PFXXX-1-05 PK-PFXXX-2-01 PK-PFXXX-2-05 PF-PFXXX PK-PFXXX-3-01 PK-PFXXX-3-05 PK-PF-CP-S PK-PF-CP-M PK-PF-CP-L * Other sizes available on request. ** XXX stands for the respective PromoFluor dye (PF405, PF415, PF488LSS, PF500LSS, etc.; see previous page). Please note: Different standard sizes are available for PF488 and PF505. Please contact us for more PromoFluor dyes not included in the list.

52 50 3 Fluorescent Labeling 3.2 PromoFluor Labeling Kits PromoFluor Biomolecules Labeling Kits Applications Labeling biomolecules such as proteins, antibodies, and nucleic acids Fluorescence microscopy Flow cytometry Fluorescent in situ hybridization (FISH) Fluorescence correlation spectroscopy (FCS) Benefits Convenient ready-to-use kits Contain superior PromoFluor dyes (see chapter 3.1) Highly efficient labeling Very bright and stable fluorescent signals PromoFluor Labeling Kits have been developed for the convenient and highly efficient labeling of target proteins or antibodies with PromoKine s superior PromoFluor dyes. They contain the respective PromoFluor NHS-Ester as well as all the other components required for labeling biomolecules. PromoFluor Labeling Kits work with all molecules with a primary amino group such as peptides, proteins, 5 -aminomodified DNA oligomers, and cdna containing aminoallyl-du. 3 Fluorescent Labeling Fig. 3: Cell structures labeled with antibodies bound to green-fluorescing PromoFluor 505 (left) and red-fluorescing PromoFluor 647 dyes (red). Illustrations kindly provided by Covalys Biosciences AG, Switzerland. Product Size Catalog Number PF-XXX-Labeling-Kit 5 reactions 10 reactions PK-PF-LK-XXX-05 PK-PF-LK-XXX-10 XXX- stands fo the respective PromoFluor dye (PF405, PF488, PF488LSS, PF514LSS, PF546, PF555, PF590, PF633, PF647, PF670, PF700, PF750, PF770; Labeling Kits with other PromoFluor dyes are available on request). Please see chapter 3.1 for details. Chapter 6.3 has a wide selection of other labeling kits (e.g. Fluorescein, Rhodamine, Sulforhodamine, R- and B-Phycoerythrin, Allophycocyanin, Biotin, Peroxidase, and Alkaline Phophatase).

53 3 Fluorescent Labeling PromoFlour DNA Labeling Kits Applications optimized for enzymatic incorporation into DNA by using a proprietary linker DNA labeling by PCR technology. The high incorporation rates DNA labeling by nick translation of the dye-dutp analogs combined with Labeling of probes for FISH, micro- the outstanding stability and quantum arrays, Southern hybridizations, etc. yield of the fluorophores make the kits the ideal choice for all typical DNA la- Benefits beling applications such as FISH, single molecule detection, microarray gene Convenient and fast DNA labeling expression profiling, and other nucleic Optimal incorporation of labeled acid hybridization assays. PromoKine s dutps PCR Labeling Kits contain all reagents Highly fluorescent and stable fluoro- required for highly efficient, rapid, and phores easy-to-perform DNA labeling (excluding primers and template DNA). The PromoKine s PCR Labeling Kits and Nick Translation Labeling Kits contain all Nick Translation Labeling Kits have been reagents required for efficient, rapid and designed for convenient direct DNA easy-to-perform DNA labeling by nick labeling by PCR or nick translation using Taq polymerase or Polymerase I/ DNase I. The fluorescently labeled dutp analogs provided with the kits have been translation (excluding template DNA). The labeled dutps are also available separately. Fig 4: PromoKine s DNA Labeling Kits have been used to generate fluorescently labeled DNA probes for staining specific 3 Fluorescent Labeling chromosome regions. Product Product Description Size Catalog Number PromoFluor-430 PCR Labeling Kit Blue-green fluorescent DNA labeling by PCR 10 reactions 50 reactions PK-PF430-PCRLK-10 PK-PF430-PCRLK-50 PromoFluor-500 PCR Labeling Kit Green-fluorescent DNA labeling by PCR 10 reactions 50 reactions PK-PF500-PCRLK-10 PK-PF500-PCRLK-50 PromoFluor-550 PCR Labeling Kit Yellow-fluorescent DNA labeling by PCR 10 reactions 50 reactions PK-PF550-PCRLK-10 PK-PF550-PCRLK-50 PromoFluor-594 PCR Labeling Kit Orange-fluorescent DNA labeling by PCR 10 reactions 50 reactions PK-PF594-PCRLK-10 PK-PF594-PCRLK-50 PromoFluor-640 PCR Labeling Kit Red-fluorescent DNA labeling by PCR 10 reactions 50 reactions PK-PF640-PCRLK-10 PK-PF640-PCRLK-50 TexasRed PCR Labeling Kit Orange-fluorescent DNA labeling by PCR 10 reactions 50 reactions PK-PFTXR-PCRLK-10 PK-PFTXR-PCRLK-50 PromoFluor-430 Nick Translation Labeling Kit Blue-green fluorescent DNA labeling by nick translation 10 reactions 50 reactions PK-PF430-NTLK-10 PK-PF430-NTLK-50 PromoFluor-500 Nick Translation Labeling Kit Green-fluorescent DNA labeling by nick translation 10 reactions 50 reactions PK-PF500-NTLK-10 PK-PF500-NTLK-50 PromoFluor-550 Nick Translation Labeling Kit Yellow-fluorescent DNA labeling by nick translation 10 reactions 50 reactions PK-PF550-NTLK-10 PK-PF550-NTLK-50 PromoFluor-594 Nick Translation Labeling Kit Orange-fluorescent DNA labeling by nick translation 10 reactions 50 reactions PK-PF594-NTLK-10 PK-PF594-NTLK-50 PromoFluor-640 Nick Translation Labeling Kit Red-fluorescent DNA labeling by nick translation 10 reactions 50 reactions PK-PF640-NTLK-10 PK-PF640-NTLK-50

54 52 3 Fluorescent Labeling 3.3 PromoFluor Labeled Biomolecules PromoFluor Labeled Antibodies Applications Fluorescence microscopy Flow cytometry Fluorescent in situ hybridization (FISH) Fluorescence correlation spectroscopy (FCS) Fig 5: Tubulin and centromere structures of HeLa cells visualized by using specific primary antibodies and secondary antibodies labeled with PromoFluor 488 and PromoFluor 555. Benefits High quality and ready-to-use Labeled with PromoFluor dyes High intensity yields and stable fluorescent signals Product Size* Catalog Number 3 Fluorescent Labeling Ready-to-use antibody conjugates of all PromoFluor dyes (see chapter 3.1.) are available as goat anti-human IgGs, goat anti-rabbit IgGs, and goat anti-mouse IgGs. The labeled secondary antibodies (polyclonal) are affinity-purified and dissolved in 100 mm PBS (ph 7.5) at a concentration of 1 mg/ml. Each antibody comes with a batch-specific absorption spectrum. PF XXX-Goat anti-mouse IgG 1 mg PK-PFXXX-AK-M1 PF-XXX-Goat anti-rabbit IgG 1 mg PK-PFXXX-AK-R1 PF-XXX-Goat anti-human IgG 1 mg PK-PFXXX-AK-H1 PF-XXX-Rabbit anti-fluorescein conj. 1 mg PK-PFXXX-AK-F1 * Other sizes available on request. XXX stands for the respective PromoFluor dye (PF405, PF488, PF488LSS, PF500LSS, PF505 etc). Please see chapter 3.1 for details PromoFluor Labeled Phalloidin Applications Localization and visualization of actin filaments Fluorescence microscopy as well as for visualizing individual actin filaments in vitro. Ready-to-use phalloidin conjugates of all PromoFluor dyes are available. Benefits High quality and ready-to-use Labeled with the superior PromoFluor dyes (see chapter 3.1) Yields highly intensive and stable fluorescent signals Fig. 6: Phalloidin and centromere structures of HeLa cells stained using a PromoFluor 647-Phalloidin conjugate and primary anti-centromer antibodies in combination with secondary antibodies labeled with PromoFluor 488. Phalloidin binds very selectively to actin (much more tightly to actin filaments than to actin monomers). In combination with conjugated PromoFluor dyes, phalloidin is enormously useful in localizing actin filaments in living or fixed cells Product Size* Catalog Number PF-XXX-Phalloidin 10 nmol PK-PFXXX-7-01 * 10 nmol are sufficient for staining approximately 300 microscopic slides. Other sizes are available on request. XXX stands for the respective PromoFluor dye (PF405, PF488, PF488LSS, PF500LSS, PF505 etc). Please see chapter 3.1 for details.

55 3 Fluorescent Labeling PromoFluor Labeled Biotin, Avidin, and Streptavidin Applications Avidin is a tetrameric glycoprotein with In addition, PromoKine also provides a combined mass of approximately 67 high-quality streptavidin and biotin con- Fluorescence microscopy kda. Due to its carbohydrate content jugates with R-Phycoerythrin (R-PE), B- Flow cytometry and basic pi, avidin shows relatively high Phycoerythrin (B-PE), Allophycocyanin Fluorescent in situ hybridization nonspecific binding. (APC), cross-linked Allophycocyanin (cl- (FISH) APC), and FITC (see chapter 3.6). Fluorescence correlation spectroscopy Streptavidin is a tetrameric 53 kda pro- (FCS) tein. Since it lacks any carbohydrate modification and has a near-neutral ph, Benefits it has the advantage of much lower nonspecific binding than avidin. On the oth- High quality and ready-to-use er hand, streptavidin is less watersoluble Labeled with superior PromoFluor than avidin. dyes (see chapter 3.1) High intensity yields and stable Ready-to-use avidin, streptavidin, and fluorescent signals biotin conjugates of all PromoFluor dyes (see chapter 3.1.) are available. The avi- Avidin and streptavidin have a very din and streptavidin conjugates are af- strong affinity for biotin with a dissociation constant of approximately mol/l. This is the highest known affinity between any protein and its finity-purified and dissolved in 100 mm PBS (ph 7.5) at a concentration of 1 mg/ ml. The biotin conjugates are affinitypurified and lyophilized. Each conjugate Fig. 7: Staining of plasma membranebound neurokinin-1 receptor (red) in a U2OS cell using a PromoFluor 547-con- 3 Fluorescent Labeling ligand. Extensive chemical modifications comes with a batch-specific absorption jugate. Picture kindly provided by Co- have little effect on this affinity. spectrum. valys Biosciences AG, Switzerland. Product Size* Catalog Number PF-XXX-Avidin Conjugate 1 mg in 1 ml PBS PK-PFXXX-4-01 PF-XXX-Streptavidin Conjugate 1 mg in 1 ml PBS PK-PFXXX-5-01 PF-XXX-Biotin Conjugate 1 mg 500 µg PK-PFXXX-6-01 PK-PFXXX * Other sizes available on request. XXX stands for the respective PromoFluor dye (PF405, PF488, PF488LSS, PF500LSS, PF505 etc). Please see chapter 3.1 for details.

56 54 3 Fluorescent Labeling PromoFluor Labeled Nucleotides Applications Fluorescent labeling of nucleic acids Fluorescent in situ hybridization (FISH) Multicolor FISH (chromosome painting) Microarrays DNA sequencing Benefits High quality and ready-to-use Labeled with superior PromoFluor dyes (see chapter 3.1) High intensity yields and stable fluorescent signals Minimal steric hindrance PromoKine provides aminoallyl-dutp (deoxyuridine-triphosphate) and aminoallyl-utp (uridine-triphosphate) conjugates for all PromoFluor dyes. The labeled nucleotides offer excellent properties with regard to signal intensity, water solubility, and stability. In addition, the low molecular weight of the dyes results in minimal steric hindrance and unproblematic incorporation into DNA/RNA using conventional enzymatic techniques like PCR/RT-PCR or in vitro transcription. Aminoallyl-dUTP can be incorporated into DNA by Taq polymerase, Klenow fragment, and reverse transcriptases whereas aminoallyl-utp can be incorporated into RNA by T3, T7, or SP6 RNA polymerases. In addition, PromoKine also provides PromoFluor labeled aminoallyl-ddutp (dideoxy-utp) which can be used for DNA sequencing. All PromoFluor labeled nucleotides are delivered as 1 mm readyto-use aqueous solutions. 3 Fluorescent Labeling Fig 8: Fluorescence laser scan of a microarray slide using PromoFluor dyes. Different matching oligonucleotides, negative controls, and reference oligonucleotides were spotted in 25 replicates and at 4 different concentrations to epoxysilane coated slides using a non-contact spotting device and hybridized with PromoFluor labeled samples. Picture kindly provided by the Functional Genomics Center Zurich, Switzerland. Fig 9: 2-color labeling of chromosomes 1 and 8 using dutps conjugated to PromoFluor 505 and PromoFluor 560. Picture kindly provided by Applied Spectral Imaging. Product Size* Catalog Number PF-XXX-aadUTP 100 nmol PK-PFXXX PF-XXX-aaUTP 100 nmol PK-PFXXX-9-100** PF-XXX-aaddUTP 100 nmol PK-PFXXX ** The range of PromoKine fluorescent labeled biomolecules is being continuously extended. Please visit regularly our website * Other sizes available on request. XXX stands for the respective PromoFluor dye (PF405, PF488, PF488LSS, PF500LSS, PF505 etc). Please see chapter 3.1 for details. ** Available on request.

57 3 Fluorescent Labeling Phycobiliprotein Dyes Applications sitive cell and molecular biological ap- 545 nm and emitting strongly at 572 nm plications such as immunofluorescence (see Fig. 11). Since it is less sticky than Labeling of biomolecules such as microscopy, flow cytometry, microarrays, R-Phycoerythrin and demonstrates less proteins, antibodies, and nucleic acids enzyme kinetic studies, etc. They show non-specific binding, B-phycoerythrin Fluorescence microscopy optimal quantum yields (up to 98%) and is better suited for applications that are Flow cytometry extinction coefficients as well as large sensitive to background signals. Fluorescent in situ hybridization (FISH) Stokes shifts. The fluorescence yield is equivalent to at least 30 unquenched Allophycocyanin Fluorescence correlation spectroscopy fluorescein or 100 rhodamine molecules (FCS) at comparable wavelengths (on a molar Allophycocyanin (APC), another member FRET assays basis), and fluorescence is not quenched of the light-harvesting phycobiliprotein Benefits by external agents since it is protected by covalent binding to the protein back- family, absorbs red light at 650 nm and has a maximal emission at 660 nm (see bone. PromoKine s phycobiliproteins Fig. 12). Allophycocyanin and its conju- Strong light absorption and high can easily be linked to antibodies. The gates are both brighter and more photo- fluorescence yields sensitivity of antibodies conjugated with stable than competing, commonly used Excellent stability and high R-PE and B-PE is usually five to ten times products. fluorescence over a broad ph range greater than that of the corresponding Fluorescent signals are not quenched by external agents Excitation and emission spectra are compatible with common excitation fluorescein conjugate. R-Phycoerythrin SMCC*-activated R-Phycoerythrin and SMCC*-activated Allophycocyanin are chemically activated (maleimide group from SMCC) for easy reactions with 3 Fluorescent Labeling sources and filter sets R-Phycoerythrin (R-PE) has a strong sulfohydryl (SH) groups such as reduced absorption peak at 565 nm and a sec- cysteine residues from proteins and Phycobiliproteins are water-soluble ondary absorption peak at 492 nm. The antibodies. The R-PE and APC labeled proteins with covalently bound chro- emission peak is at 575 nm (see Fig. antibodies are highly fluorescent and mophores present in cyanobacteria 10). This protein is commonly used for ideally suited for applications such as and certain algae (rhodophytes, cryp- fluorescent immunolabeling, particularly immunofluorescence microscopy or flow tomonads, glaucocystophytes) that for applications involving fluorescence- cytometry. capture light energy during photosynthesis. The major phycobiliproteins are R-Phycoerythrin, B-Phycoerythrin, and Allophycocyanin. Phycobiliproteins can be used as fluorescent dyes to label biomolecules to obtain activated cell sorting (FACS). B-Phycoerythrin B-Phycoerythrin (B-PE) has slightly different spectral characteristics than R-Phy- * Succinimidyl-4-(N-Maleimidomethyl)Cyclohexane- 1-Carboxylate (SMCC) is a non-cleavable, membranepermeable crosslinker containing an amine-reactive N- hydroxysuccinimide (NHS ester) and a sulfhydryl-reactive maleimide group. NHS esters react with primary amines at ph 7-9 to form stable amide bonds, while maleimides react with sulfhydryl groups at ph to form stable thioether bonds. specific probes or markers for highly sen- coerythrin absorbing strongly at about Excitation / Emission [%] Fig. 10: Excitation (left) and emission (right) spectra of 20 R-Phycoerythrin Wavelength [nm]

58 56 3 Fluorescent Labeling Excitation / Emission [%] Fig. 11: Excitation (left) and emission (right) spectra of B-Phycoerythrin Wavelength [nm] Excitation / Emission [%] 3 Fluorescent Labeling Fig. 12: Excitation (left) and emission (right) spectra of Allophycocyanin Wavelength [nm] Product Size Catalog Number R-Phycoerythrin, lyophilized 5 mg PK-PF-RPE-05 SMCC-activated R-Phycoerythrin, lyophilized 5 mg PK-PF-IRPE-05 B-Phycoerythrin, lyophilized 5 mg PK-PF-BPE-05 Allophycocyanin, lyophilized 5 mg PK-PF-APC-05 Allophycocyanin (cross-linked), lyophilized 5 mg PK-PF-APC-05CL SMCC-activated Allophycocyanin, lyophilized 5 mg PK-PF-IAPC-05 We also provide high quality streptavidin and biotin conjugates with R-Phycoerythrin, B-Phycoerythrin, Allophycocyanin, and cross-linked Allophycocyanin. Please see chapter 3.6 for more information.

59 3 Fluorescent Labeling Phycobiliprotein Labeling Kits Applications cent phycobiliproteins R-Phycoerythrin, B-Phycoerythrin, and Allophycocyanin. SH-reactive Phycobiliprotein Labeling Kits Labeling biomolecules such as The kits contain all necessary reagents proteins and antibodies for labeling, including storage buffer and The SH-reactive Phycobiliprotein Label- Fluorescence microscopy filtration tubes for quick buffer exchange ing Kits include SH-reactive phycobili- Flow cytometry and concentration. They are capable of proteins with maleimide groups and Fluorescent in situ labeling for example up to 200 µg IgG.* can easily form covalent bonds with the hybridization (FISH) sulfhydryl groups of target molecules Fluorescence correlation spectroscopy (FCS) Benefits NH 2 -reactive Phycobiliprotein Labeling Kits without any activation process. The sensitivity of the conjugates is higher than those labeled with the NH 2 -reactive Phycobiliprotein Labeling Kits due to the The NH 2 -reactive phycobiliproteins in- site-specific labeling reaction. However, Convenient ready-to-use kits cluded in the kits have activated ester the SH-type labeling procedure requires High recovery of conjugates groups that can be coupled to primary a reducing reaction to create SH group(s) All steps in a single filtration tube or secondary amino groups of target prior to the labeling step. If sensitivity of molecules. There is no activation process the conjugate is the most important fac- PromoKine s Phycobiliprotein Labeling necessary which makes the protocol very tor, we recommend using the SH type Kits have been developed for the convenient and efficient labeling of target proteins and antibodies with the fluores- fast and easy to perform. The sensitivity of the labeled target molecules is suitable for most applications. kits. 3 Fluorescent Labeling Product Product Description Size Catalog Number B-Phycoerythrin Labeling Kit-NH 2 B-Phycoerythrin Labeling Kit-SH R-Phycoerythrin Labeling Kit-NH 2 R-Phycoerythrin Labeling Kit-SH Allophycocyanin Labeling Kit-NH 2 Allophycocyanin Labeling Kit-SH Kit for Labeling of Proteins/Antibodies with B-Phycoerythrin Kit for Labeling of Proteins/Antibodies with B-Phycoerythrin Kit for Labeling of Proteins/Antibodies with R-Phycoerythrin Kit for Labeling of Proteins/Antibodies with R-Phycoerythrin Kit for Labeling of Proteins/Antibodies with Allophycocyanin Kit for Labeling of Proteins/Antibodies with Allophycocyanin 3 reactions PK-PF-BPE-LK22** 3 reactions PK-PF-BPE-LK25** 3 reactions PK-PF-RPE-LK23** 3 reactions PK-PF-RPE-LK26** 3 reactions PK-PF-APC-LK21** 3 reactions PK-PF-APC-LK24** * Note: Proteins > 10 kda added as stabilizers (e.g. BSA or gelatin) have to be removed prior to the labeling reaction. PromoKine offers the respective antibody purification kits on request. ** Not available in Japan, China, and Korea

60 58 3 Fluorescent Labeling 3.6 Phycobiliprotein Labeled Biomolecules Applications Fluorescence microscopy Flow cytometry Fluorescent in situ hybridization (FISH) Fluorescence immunoassay (FIA) Benefits High quality Ready-to-usee High intensity yields and stable fluorescent signals Avidin and streptavidin have a very strong affinity for biotin with a dissociation constant of approximately mol/l. This is the highest known affinity between any protein and its ligand. Extensive chemical modifications have little effect on this affinity. Avidin is a tetrameric glycoprotein with a combined mass of approximately 67 kda. Due to its carbohydrate content and basic pi, avidin shows relatively high nonspecific binding. Streptavidin is a tetrameric 53 kda protein. Since it lacks any carbohydrate modification and has a near-neutral ph, it has the advantage of much lower nonspecific binding than avidin. On the other hand, streptavidin is less water soluble than avidin. Ready-to-use avidin, streptavidin, and biotin conjugates of R-Phycoerythrin, B-Phycoerythrin, and Allophycocyanin are available. These ready-to-use conjugates are very useful second-step reagents for staining with biotinylated or streptavidin-coupled antibodies and can be used in (strept)avidin/biotin-labeling systems for flow cytometry, fluorescent immunoassays, immunohistochemistry, and immunofluorescence microscopy. 3 Fluorescent Labeling Product Size* Catalog Number Streptavidin-R-Phycoerythrin Streptavidin-B-Phycoerythrin Streptavidin-Allophycocyanin Streptavidin-Allophycocyanin (cross-linked) Biotin-R-Phycoerythrin Biotin-B-Phycoerythrin Biotin-Allophycocyanin 1 mg in 1 ml Buffer 5 mg in 5 ml Buffer 1 mg in 1 ml Buffer 5 mg in 5 ml Buffer 1 mg in 1 ml Buffer 5 mg in 5 ml Buffer 1 mg in 1 ml Buffer 5 mg in 5 ml Buffer 1 mg in 1 ml Buffer 5 mg in 5 ml Buffer 1 mg in 1 ml Buffer 5 mg in 5 ml Buffer 1 mg in 1 ml Buffer 5 mg in 5 ml Buffer PK-PF-SA-RPE-01 PK-PF-SA-RPE-05 PK-PF-SA-BPE-01 PK-PF-SA-BPE-05 PK-PF-SA-APC-01 PK-PF-SA-APC-05 PK-PF-SA-APC-01CL PK-PF-SA-APC-05CL PK-PF-BT-RPE-01 PK-PF-BT-RPE-05 PK-PF-BT-BPE-01 PK-PF-BT-BPE-05 PK-PF-BT-APC-01 PK-PF-BT-APC-05 Biotin-Allophycocyanin (cross-linked) 1 mg in 1 ml Buffer PK-PF-BT-APC-01CL Streptavidin-Fluorescein-5-isothiocyanate (FITC) 1 mg in 1 ml Buffer 5 mg in 5 ml Buffer PK-PF-SA-FITC-01 PK-PF-SA-FITC-05

61 3 Fluorescent Labeling PromoFluor Antifade Reagent Applications Mounting medium for fluorescence microscopy Benefits Significantly increased photostability Compatible with many common dyes Conserves cell preparations Convenient packaging Irreversible photobleaching processes lead to a loss of fluorescent signal sensitivity particularly when high intensity excitation light is used for a longer duration, and the target molecules in samples are in low abundance. PromoFluor Antifade Reagent significantly increases the photostability of fluorochromes in fixed cells, fixed tissues, and cell-free preparations. It is compatible with many common dyes and dye complexes resulting in prolonged signals and improved image contrast especially in multicolor applications. Moreover, it gently solidifies after the attachment of the cover slide, permanently conserving the cell preparation and fixing the coverslip. PromoFluor Anitfade Reagent makes slide immobilization using e.g. nail polish unnecessary. Samples can be saved for months after mounting, retaining fluorescence during prolonged storage. PromoFluor Antifade Reagent comes premixed in a convenient dropper bottle. Fig. 13: Immunofluorescence imaging of a human keratinocyte stained with FITC-labeled antibodies without (top) and with (bottom) PromoFluor Antifade Reagent at different excitation intervals (5, 10, 15, 20, 25 seconds). 3 Fluorescent Labeling Product Product Description Size Catalog Number PromoFluor Antifade Reagent Mounting Medium for Fluorescence Microscopy 8 ml PK-PF-AFR1

62

63 Gene Cloning, Expression, 4 and Silencing Gene cloning is the process of making multiple copies of a defined DNA sequence. Since its establishment it has become a core technique in molecular biology, and it enables a wide array of biological experiments and practical applications. Amplifying DNA has also allowed the study of gene expression, including the examination of genetic information transcribed and translated into RNA and proteins. In contrast, gene silencing has also grown into an important technique for analyzing gene functions.

64 62 Contents Chapter 4 4 Gene Cloning, Expression, and Silencing 4.1 Plasmid Vectors ppk-cmv Expression Vectors ppk-cmv Reporter Vectors ppk-cmv Fusion Vectors Gene Cloning and Expression Kits FastClone PCR Cloning Kits FastExpress Gene Expression Kits StarGate Cloning Systems StarGate Combinatorial Cloning System StarGate Mutagenesis System StarGate Fusion Cloning System Gene Silencing GeneOFF sirna Expression Kits GeneOFF shrna Vector Kits GeneOFF sirna Generation Kit... 74

65 4 Gene Cloning, Expression, and Silencing Plasmid Vectors ppk-cmv Expression Vectors Applications Constitutive high-level transgene expression Expression of native and HA-tagged proteins Benefits Optimized CMV promoter provides high level constitutive expression Suited for a wide variety of cell types for both stable and transient gene expression Small vector size ensures high transfection efficiency Multiple cloning site with most commonly-used restriction sites The ppk-cmv Expression Vectors contain a modified human cytomegalovirus (CMV) based promoter/enhancer sequence that has been optimized for high gene expression. Sequence motives that limit expression have been removed, while others that promote high expression levels have been retained. Consequently, these vectors yield significantly higher expression levels than other expression vectors such as pcdna3.1 that are based on the standard CMV promoter. In addition, the plasmid backbones are optimized to allow for very small vector sizes and higher plasmid yields when amplified in E. coli. Three versions of the ppk-cmv Expression Vector are available. ppk-cmv-e1 does not have an expression tag. It is intended for expressing recombinant proteins in the native forms. ppk-cmv-e2 comes with an N- terminal HA (hemagglutinin) fusion tag. ppk-cmv-e3 comes with a C-terminal HA-fusion tag. The HA fusion tags allow easy detection and purification of proteins. All three Expression Vectors come with a positive control vector containing a CAT gene. ppk-cmv-e1 ppk-cmv-e2 ppk-cmv-e3 puc ori ATG Bgl II Xho I Hind III EcoR I Kpn I Apa I Sma I BamH I Not I Xba I HA Bgl II Xho I Hind III EcoR I Kpn I Apa I Sma I BamH I CMV Bgl II Xho I Hind III EcoR I Kpn I Apa I Sma I BamH I Not I Xba I ppk-cmv Expression Vector 4.2 kb HSV pa SV40pA Kan/Neo SV40p Fig. 1: Three versions of the ppk-cmv Expression Vectors are available for convenient and efficient gene cloning and expression. HA Term AmpP 4 Gene Cloning, Expression, and Silencing Product Size Catalog Number ppk-cmv-e1 25 μg PK-MB-P ppk-cmv-e2 25 μg PK-MB-P ppk-cmv-e3 25 μg PK-MB-P003300

66 64 4 Gene Cloning, Expression, and Silencing ppk-cmv Reporter Vectors 4 Gene Cloning, Expression, and Silencing Applications Transient, high-level reporter gene/ target gene expression in mammalian cells and tissues Monitoring transfection efficiency and gene expression Benefits High expression of five popular reporter genes Suited for in vitro and in vivo gene expression Convenient multiple cloning site for optimized cloning and expression of target genes Suited for a wide variety of cell types Small vector size ensures high trans- fection efficiency and high plasmid yield in E. coli The ppk-cmv Reporter Vectors have been designed for high level, transient expression of reporter or target genes. They contain an optimized CMV promoter followed by intron A from the CMV immediate-early gene (IE). This ensures extremely high transient expression in a wide variety of mammalian cells and tissues. In addition, the vectors contain an optimized plasmid backbone that has been extensively altered to achieve optimal plasmid production in E. coli. The vectors are available without a reporter gene to clone and express a gene of interest (blank vector) as well as with the following reporter genes: β-galactosidase (lacz), green fluorescent protein (GFP), luciferase (Luc), chloramphenicol acetyltransferase (CAT), and secreted alkaline phosphatase (SEAP). ppk-cmv Reporter Vector 4.2 kb Fig. 2: The ppk-cmv Reporter Vector allows strong transient expression of target genes as well as different reporter genes in many cell types and tissues. Product Size Catalog Number Reporter MCS puc ori Acc I Sal I Eco72 I Bcl I EcoR V Not I Bbe I Ehe I Kas I Nar I BamH I CMV b-gal GFP Luciferase CAT SEAP ppk-cmv-r0 (Blank) 25 μg PK-MB-P ppk-cmv-r1 (b-gal) 25 μg PK-MB-P ppk-cmv-r2 (CAT) 25 μg PK-MB-P ppk-cmv-r3 (Luc) 25 μg PK-MB-P ppk-cmv-r4 (GFP) 25 μg PK-MB-P ppk-cmv-r5 (SEAP) 25 μg PK-MB-P HSV pa SV40pA Kan AmpP SV40p Please see chapter 1 for luciferase, beta-gal, and GFP reporter assays.

67 4 Gene Cloning, Expression, and Silencing ppk-cmv Fusion Vectors Applications efficient selection in both E. coli (using ppk-cmv-f4 (N-Luc) has a Luciferace kanamycin) and mammalian cells (using coding sequence without a stop codon Constitutive high-level GFP or neomycin). inframe in the MCS. The gene of inter- Luciferase fusion protein expression An SV40 polyadenylation signal provides est can therefore be expressed with Lu- Creating GFP or Luciferase efficient transcription termination and ciferase at the N-terminus. expressing stable cell lines polyadenylation - thus ensuring stable mrna. A puc origin of replication al- Benefits Optimized CMV promoter provides high level constitutive expression Suited for a wide variety of cell types lows for high copy numbers in E. coli. The vectors can be used for either transient transfection or for generating stable cell lines expressing only the reporter gene or the fusion target-reporter gene ppk-cmv-f1 (C-GFP, 4965 bp) ppk-cmv-f2 (N-GFP, 4962 bp) Pst I (2) Bgl II Xho I Hind III EcoR I (2) Pst I (2) Bgl II Xho I Hind III EcoR I (2) GFP GFP EcoR I (2) Pst I (2) Kpn I Apa I Sma I Xma I BamH I Not I Xba I Stop EcoR I (2) Pst I (2) Kpn I Apa I Sma I Xma I BamH I Not I Xba I Small vector size ensures high construct. transfec tion efficiency Two multiple cloning sites upstream or downstream of the reporter gene The ppk-cmv Fusion Vectors have been Four versions of the vector are available for convenient and efficient gene cloning and expression of the target gene fused with a reporter gene: ppk-cmv-f1 (C-GFP) has a GFP cod- ppk-cmv-f3 (C-Luc, 5898 bp) ppk-cmv-f4 (N-Luc, 5895 bp) Pst I (2) Bgl II Xho I Hind III EcoR I (2) Pst I (2) Bgl II Xho I Hind III EcoR I (2) LUC LUC EcoR I (2) Pst I (2) Kpn I Apa I Sma I Xma I BamH I Not I Xba I Stop EcoR I (2) Pst I (2) Kpn I Apa I Sma I Xma I BamH I Not I Xba I designed for high-level expression of ing sequence with a stop codon inframe GFP or Luciferase fusion proteins and for creating GFP or Luciferase express- in the MCS. The gene of interest can therefore be expressed with GFP at the + Intron pt7 SV40pA ing stable cell lines. They contain an C-terminus. optimized CMV promoter and an intron sequence, which ensures extremely high constitutive expression in a wide variety of mammalian cell types. Due to two multiple cloning sites on either side of the reporter gene, N- or C-terminal fusion proteins can easily ppk-cmv-f2 (N-GFP) has a GFP coding sequence without a stop codon inframe in the MCS. The gene of interest can therefore be expressed with GFP at the N-terminus. ppk-cmv-f3 (C-Luc) has a Luciferase coding sequence with a stop codon in- puc ori ppk-cmv Fusion Vector HSV pa Kan/Neo AmpP SV40p 4 Gene Cloning, Expression, and Silencing be generated. In addition, each vector frame in the MCS. The gene of interest Fig. 3: The ppk-cmv Fusion Vectors are contains a combined kanamycin/neo- can therefore be expressed with Lu- extremely versatile, and their compact mycin resistance gene, which allows for ciferase at the C-terminus. size insures high transfection efficiency. Product Size Catalog Number ppk-cmv-f1 (C-GFP) 20 μg PK-MB-P ppk-cmv-f2 (N-GFP) 20 μg PK-MB-P ppk-cmv-f3 (C-Luc) 20 μg PK-MB-P ppk-cmv-f4 (N-Luc) 20 μg PK-MB-P Please see chapter 1 for luciferase, beta-gal, and GFP reporter assays.

68 66 4 Gene Cloning, Expression, and Silencing 4.2 Gene Cloning and Expression Kits FastClone PCR Cloning Kits Applications virus (CMV) based promoter/enhancer PromoKine OptiCells Competent sequence that has been optimized for E. coli cells are recommended for use Fast, convenient, and direct PCR high-level expression in different mam- with the FastClone PCR Cloning Kits. cloning malian cells. These specially optimized competent High-level gene expression in Three versions of the FastClone PCR cells provide transformation efficiencies mammalian cells Cloning Kit are available: of >1x10 9 cfu/µg, even under the most The FastClone PCR Cloning Kit 1 con- difficult transformation conditions, such Benefits tains the linear expression vector ppk- as when a large amount of DNA is used CMV-E1/FC without any tag. for transformation, when diluted DNA No need for restriction The FastClone PCR Cloning Kit 2 con- samples are used, or when a high salt li- or ligation enzymes tains the linear expression vector ppk- gation mix is used. OptiCells Competent Superior cloning efficiency CMV-E2/FC with an N-terminal HA fu- E. coli Cells are available separately. Available for native or HA-tagged sion tag. protein expression The FastClone PCR Cloning Kit 3 contains the linear expression vector ppk- The FastClone PCR cloning method CMV-E3/FC with a C-terminal HA fusion saves hours of work because it eliminates tag. ppk-cmv-e1/fc the need for restriction or ligation enzymes. PCR-amplified genes are simply ppk-cmv-e2/fc ATG HA mixed with linearized FastClone vectors 4 Gene Cloning, Expression, and Silencing and transformed into highly competent E. coli DH5a. Typically, more than 85% of the resultant colonies contain clones with the relevant insert in the correct orientation for expression. Because the FastClone vectors do not need multiple cloning sites or other enzyme recognition sites (e.g., a topoisomerase I recognition site), no unwanted sequences are incorporated into the resulting expression vector. This is 5 FastClone Ends 3 FastClone Ends FastClone Vector (linear) PCR Fragment Transform Homologous Recombination OptiCells E. coli Plate FastClone Vector (linear) Gene of Interest Recombinant Plasmid Kanamycin Resistance Gene ppk-cmv-e3/fc puc ori CMV HA SV40pA ppk-cmv FastClone Vectors 4.2 kb SV40p AmpP especially important when it is necessary to minimize the risk of structural or Select recombinants on plates containing kanamycin HSV pa Kan/Neo functional interferences. The ppk-cmv FastClone vectors included in the kit Fig. 4: The FastClone PCR Cloning Kits Fig. 5: The FastClone PCR Cloning vec- contain an efficient SV40 terminator as provide an optimal tool for fast, direct tors are perfectly qualified for conve- well as a modified human cytomegalo- PCR cloning. nient gene cloning and expression. Product Size Catalog Number FastClone PCR Cloning Kit 1 20 Reactions PK-MB-FC FastClone PCR Cloning Kit 2 20 Reactions PK-MB-FC FastClone PCR Cloning Kit 3 20 Reactions PK-MB-FC OptiCells Competent E. coli Cells 20 x 50 µl PK-MB-C OptiCells Competent E. coli F' Cells 20 x 50 µl PK-MB-C101120

69 4 Gene Cloning, Expression, and Silencing FastExpress Gene Expression Kits Applications Fast and easy generation of PCR fragments ready for gene expression High-throughput screening of functional gene expression Rapid screening of genes for intracellular localization studies Benefits Because FastExpress eliminates the need for classic gene cloning, bacterial transformations, and plasmid purifications, large numbers of genes can be shotgun expressed and examined simultaneously. This allows for extremely quick experiments with very little processing time. The FastExpress Gene Expression Kits are available in two versions. One generates a FastExpress fragment without a tag. The other can be used to add an N-terminal hemagglutinin (HA) tag to the 5 end of FastExpress-amplified genes. This HA tag considerably simplifies downstream processes such as antigen and cdna library screening, studying intracellular localization, and many other functional genomics applications. Excellent gene expression in vitro and in vivo Suited for all genes Available with an HA tag for easy detection and purification of target proteins 5 FastExpress End Sequence 5 Custom Oligo Gene of Interest First Step PCR FastExpress Primary Fragment 3 Custom Oligo 3 FastExpress End Sequence The FastExpress Gene Expression System is an innovative method for highthroughput gene expression. It makes time-consuming and tedious gene cloning unnecessary by adding a strong eukaryotic promoter and terminator sequence to any target gene of interest via a simple 2-step PCR procedure. The linear FastExpress fragments generated by the FastExpress 2-step PCR are transcriptionally active and can be expressed in vitro and in vivo. The obtained expression levels are similar to those typically observed with conventional plasmid vectors. FastExpress Promoter FastExpress Terminator FastExpress 5 Primer Second Step PCR FastExpress 3 Primer FastExpress Promoter FastExpress Fragment FastExpress Terminator Delivery into cells or animals Fig. 6: The FastExpress Gene Expression Kits provide an innovative technique for gene expression and protein detection without tedious classical gene cloning steps. 4 Gene Cloning, Expression, and Silencing Product Size Catalog Number FastExpress Gene Expression Kit FastExpress Gene Expression Kit (HA) 20 Reactions 100 Reactions 20 Reaction 100 Reactions PK-MB-FE10210 PK-MB-FE10110 PK-MB-FE10220 PK-MB-FE10120

70 68 4 Gene Cloning, Expression, and Silencing 4.3 StarGate Cloning Systems StarGate is a very efficient system for cloning, expressing, mutating, and fusing genes or gene fragments. It allows easy gene transfer among a variety of high- level expression vectors, which have been optimized for diverse host systems StarGate Combinatorial Cloning System Applications Fig. 7: Principle of StarGate Combinatorial Cloning: Convenient gene cloning in expression GENE The gene of interest is amplified by PCR vectors optimized for different hosts Easy screening for optimal tags, PCR Primer using a set of phosphorylated primers with StarGate combinatorial sites promoters, and host systems GENE (4 bases) at the 5 end. Benefits StarCombinase TM Open Entry Vector The phosphorylated PCR product, Star- Combinase, and a ready-to-use linear and dephosphorylated entry vector are E. coli, mammalian, yeast, and mixed. In a fast one-tube combinatorial baculovirus vectors available reaction, the PCR product is introduced Numerous vectors with different tags and promoters available One-Tube Reaction in the entry vector generating the donor vector. Highly efficient one-tube reaction After propagating the donor vector in Extremely short combinatorial sites GENE E. coli the gene of interest can be sub- 4 Gene Cloning, Expression, and Silencing Cost-effective and royalty-free Blue/white screening Efficient procedures for generating functional recombinant proteins or protein complexes are of key importance in stateof-the-art life sciences. A large range of tools such as different promoters, tags StarCombinase TM Donor Vector One-Tube Reaction Acceptor Vector An A3 A2 A1 cloned into different Acceptor Vectors with the desired features, like host-specific promoters or specific tags. To create the Destination Vector the donor vector with the gene of interest is mixed with StarCombinase and the Acceptor Vectors. In a fast, one-tube combinatorial reaction, the PCR product is introduced and host systems are available for ex- in the respective Acceptor Vectors. pressing, purifying, detecting, or immobi- The resulting Destination Vectors are lizing recombinant proteins. Due to the diverse nature of proteins, it GENE Dn D3 D2 then transformed into the corresponding host cells for further applications (e.g. is impossible to predict which combination of these tools will perform best. Destination Vector D1 protein purification). E. coli clones harbouring the desired Destination Vector PromoKine now provides the StarGate with the gene of interest can be easily Combinational Cloning System to sys- detected using blue/white selection. temize and accelerate the initial search for the most successful tools. The Star- Gate system offers fast and convenient host-specific promoters, purification tags, specific combinatorial enzyme StarCom- subcloning of an arbitrary target gene of or signal sequences for protein secretion binase, that is included in the StarSolu- interest in many different vectors, and it into the culture medium. This allows for tions and makes cloning fast, easy, and allows the systematic combination of pro- efficient expression of the target protein efficient. Optimal cloning efficiency is moters, tags, and other genetic elements. with useful features in different hosts. ensured by a directed reaction. The gene of interest is initially cloned into Because the Donor and Acceptor Vectors The StarGate technology also provides a Donor Vector to simultaneously fuse have extremely short combinatorial sites easy generation of fusion proteins and it with many different genetic surround- (4 bases only) and correlating features, a versatile mutagenesis system with the ings via transfer into Acceptor Vectors the exact same protein sequence can be StarPrimerD Signer software. to generate Destination Vectors. Each expressed in different hosts with minimal The ready-to-use kits contain all required Acceptor Vector provides optimized con- modification to the gene of interest. The components to subclone the gene of in- ditions for the respective application, e.g., core element of the system is the site- terest.

71 4 Gene Cloning, Expression, and Silencing 69 StarGate Entry Cloning Set StarGate Transfer Reagent Set Acceptor Vectors The StarGate Entry Cloning Set includes the Entry Vector pentry-iba10, StarSolution containing StarCombinase for 20 reactions, For/Rev Seqencing Primers, competent E. coli Top10 cells (for 20 transformations), a DNA ladder, and the StarPrimer D Signer Software. The StarGate Transfer Reagent Set is used to transfer the genes of interest from the Entry Vector directly into different Acceptor Vectors. You can choose from our wide range of sophisticated Acceptor Vectors specifically designed for your requirements. The set includes StarSolutions containing StarCombinase for 20 reactions. You can choose from a wide range of differing StarGate acceptor vectors specifically designed for your required application. The Acceptor Vectors are available separately and come ready-to-use for 5 transfer reactions. For a detailed description of the currently available StarGate vectors please see: StarGate Mutagenesis System Applications Fig. 8: Principle of StarGate Muta- genesis: Site-specific modification of a target gene GENE Mutation Two parts of the gene of interest are amplified by PCR using two designed Benefits Easy generation of gene mutations Fully compatible with the StarGate Combinatorial Cloning System Highly efficient one-tube reaction Parallel automated mutagenesis StarCombinase TM 2 x PCR Primer Open Entry Vector primer sets which overlap at the location where a mutation should be introduced. Both primer sets have StarGate combinatorial sites (4 bases) at the 5 end. The two PCR products, StarCombinase, and ready-to-use linear and dephosphorylated Entry Vector pentry-iba are mixed. In a fast one-tube combi- 4 Gene Cloning, Expression, and Silencing possible natorial reaction the PCR products are Easy handling introduced in the entry vector generating the Donor Vector which contains the The StarGate Mutagenesis System is a mutated gene. convenient tool for site-specific modifi- One-Tube Reaction After propagating the Donor Vector in cation of a target gene and subsequent E. coli the mutated gene of interest can cloning of the modified gene. The de- Mutation be subcloned into different Acceptor sired mutation can be inserted within GENE Vectors using the StarGate Combinato- a sequence stretch of up to 80 bp. The procedure is similar to the StarGate Donor Vector rial Cloning System. After transformation, E. coli clones har- Combinatorial Cloning System with the bouring the desired Donor clone with exception that two PCRs have to be per- the mutated gene of interest can be eas- formed instead of a single PCR. The two ily detected using blue/white selection. primer sets can easily be designed with the StarPrimer D Signer Software, which is included in the kit. To design the primers, the sequence of StarGate Mutagenesis Entry Cloning Set Top10 cells (5 transformations), a DNA ladder, and the StarPrimer D Signer Soft- the gene of interest (as present in the ware. This is sufficient to create up to 5 template) as well as the desired mutated The StarGate Mutagenesis Entry Clon- different Donor Vectors containing the gene sequence simply have to be pasted ing Set includes the Entry Vector pen- mutated genes of interest. Afterwards, into the respective StarPrimer D Signer TRY-IBA20, StarSolutions containing you use the StarGate Transfer Reagent sequence windows. The software then StarCombinase for 5 reactions, For/Rev Set as described above. displays the required primer sequences. Sequencing Primers, competent E. coli

72 70 4 Gene Cloning, Expression, and Silencing StarGate Fusion Cloning System Applications troducing an internal ribosomal entry site (IRES), a Shine-Dalgarno sequence, or an StarGate Fusion Cloning Set 2 (SD1) Generation of fusion proteins animo acid linked sequence between the Polycistronic gene expression in two genes. This enables the construction The StarGate Fusion Cloning Set 2 (SD1) mammalian cells of polycistronic genes, artificial operons, has a Shine-Dalgarno (SD) sequence for Construction of artificial operons for or fusion proteins respectively. the construction of artificial operons in multiple gene expression in bacterial Since StarGate Fusion Cloning can be E. coli. The Set includes a StarGate Fu- cells performed repeatedly, it is even possible sion Reagent Set (pentry-iba20; 5 Gene function and protein interaction to generate large constructs consisting reactions), Downstream Fusion Vector studies of several genes. For this purpose, all pcfuse-iba1 (5 reactions), StarSolutions Benefits vectors are also available separately on request. including Star Combinase (for 5 fusions), Upstream Fusion Vector pnfuse-iba- SD1 (5 reactions), and competent E. coli Easy and fast generation of stable fusion proteins Compatible with the StarGate Combinatorial Cloning System StarGate Fusion Cloning Set 1 (IRES1) The StarGate Fusion Cloning Set 1 TOP10 cells. StarGate Fusion Cloning Set 3 (LINK1) Highly efficient one-tube reactions (IRES1) provides an internal ribosomal Easy handling entry site (IRES) for polycistronic gene The StarGate Fusion Cloning Set 3 expression in mammalian cells from one (LINK1) provides an amino acid linker StarGate Fusion Cloning is an optional expression vector. The set includes a (GSGGGSGGGS) for the generation intermediate step between the StarGate StarGate Fusion Reagent Set (pentry- of fusion proteins. The Set includes 4 Gene Cloning, Expression, and Silencing Entry Reaction and the Transfer Reaction. It allows easy and fast fusion of two genes of interest present in separate Donor Vectors by performing two sequential StarGate Subcloning Reactions. In addition, it offers the possibility of in- IBA20; 5 reactions), Downstream Fusion Vector pcfuse-iba1 (5 reactions), StarSolutions including StarCombinase (for 5 fusions), Upstream Fusion Vector pnfuse-iba-ires1 (5 reactions), and competent E. coli TOP10 cells. StarGate Fusion Reagent Set (pentry- IBA20; 5 reactions), Downstream Fusion Vector pcfuse-iba1 (5 reactions), StarSolutions including StarCombinase for 5 fusions, Upstream Fusion Vector pnfuse-iba-link1 (5 rxns), and competent E. coli TOP10 cells. lac P/Za Upstream Fusion Vector StarCombinase TM lac P/Za Gene 1 Upstream Fusion Vector Gene 1 Gene 1 Donor Vector Gene 1 StarCombinase TM G1 link G2 Donor Fusion Vector Gene 1/Gene 2 Gene 2 Donor Vector Gene 2 StarCombinase TM Gene 2 Downstream Fusion Vector Gene 2 lac P/Za Entry Vector Acceptor Vectors lac P/Za Downstream Fusion Vector Fig. 9: Principle of StarGate Fusion Cloning: The procedure starts with two StarGate Donor Vectors containing the genes or gene fragments to be fused. Through two separate subcloning reactions, the genes are transferred to both upstream Fusion Vector pnfuse-iba that contains the intergenic region (IRES, Shine-Dalgarno, or an amino acid linker) and to the common downstream Fusion Vector pcfuse-iba1. This is done in a fast one-tube combinatorial reaction and results in the generation of the Upstream and Downstream Fusion Vectors containing Gene 1 and Gene 2. After propagating the two vectors in E. coli the genes in the upstream and downstream fusion vectors are assembled in the Entry Vector (pentry- IBA20). This is again done in a fast onetube combinatorial reaction. The fused genes with the desired intergenic region are now ready to be subcloned into any of the StarGate Acceptor Vectors. If necessary, the procedure can be repeated to add more genes or gene fragments.

73 4 Gene Cloning, Expression, and Silencing 71 Standard Entry Reaction StarGate Entry Cloning Set Mutagenesis Entry Reaction StarGate Mutagenesis Entry Cloning Set Donor Vector Fusion Cloning StarGate Fusion Cloning Set Acceptor Vectors A1 A2 A3 An Transfer Reaction StarGate Transfer Reagent Set Destination Vectors D1 D2 D3 Dn Acceptor Vectors of choice 4 Gene Cloning, Expression, and Silencing Different Hosts Fig. 10: Overview of the StarGate Cloning System Product Size Catalog Number StarGate Entry Cloning Set 1 set PK-MB StarGate Transfer Reagent Set 1 set PK-MB StarGate Acceptor Vectors 5 reactions PK-MB-P4XXX Competent E. coli TOP10 Cells 20 transformations PK-MB StarGate Mutagenesis Entry Cloning Set 1 set PK-MB StarGate Fusion Cloning Set 1 (IRES1) 5 reactions PK-MB StarGate Fusion Cloning Set 2 (SD1) 5 reactions PK-MB StarGate Fusion Cloning Set 3 (LINK1) 5 reactions PK-MB The StarGate Cloning Systems are distributed for IBA GmbH, Germany

74 72 4 Gene Cloning, Expression, and Silencing 4.4 Gene Silencing GeneOFF sirna Expression Kits Applications Upstream Primer Upstream Primer High-level, convenient expression of sirna for gene silencing Benefits pu6 or ph1 PCR sense Template DNA Downstream primer with sense sirna pu6 or ph1 Template DNA antisense Downstream primer with antisense sirna PCR Fast and easy expression of sirnas in vitro without cloning Allows extremely cost-effective screening of up to 20 sirnas Available with reporter gene tracking pu6 or ph1 sense pu6 or ph1 antisense antisense sense Mix, Purify, and Transfect 4 Gene Cloning, Expression, and Silencing The PromoKine GeneOFF sirna Expression Kits provide a rapid, convenient, and highly effective way to produce sequence specific sirnas (small interfering RNAs) in vitro without the need for PCR cloning. The desired sirna expression cassettes are generated by a one-step PCR. They are subsequently transfected into cells, where they are transcribed to produce sense and anti-sense sirnas. They anneal in situ to form the duplex sirnas that effectively mediate gene silencing*. There are two GeneOFF sirna Expression Kits available containing DNA templates with the U6- or the H1-RNA polymerase III promoters. This allows optimal sirna transcription in a wide variety of cell types. In addition, two variants of these kits are available with a GFP reporter gene cassette to allow easy determination of the PCR fragment s transfection efficiency. pcmv Gene Silencing of mrna Upstream Primer GFP pcmv GFP pu6 or ph1 PCR pu6 or ph1 sense sirnas Template DNA Transcription Upstream Primer Downstream primer with sense sirna sense antisense Annealing (in situ) pu6 or ph1 Mix, Purify, and Transfect RNAs (sense and antisense) Template DNA antisense Downstream primer with antisense sirna PCR pcmv GFP pu6 or ph1 antisense sense * See chapter 5 for information about our sirna transfection reagents Fig. 11: Principle of Gene Silencing GFP mrna Gene Silencing of mrna GFP Transcription RNAs (sense and antisense) with PCR-generated sirnas using the PromoKine GeneOFF sirna Expression Kit, without (above) and with (below) sirnas Annealing (in situ) GFP reporter gene. Product Size Catalog Number GeneOFF sirna Expression Kit (H1) 20 rxn PK-MB-P GeneOFF sirna Expression Kit (H1-GFP) 20 rxn PK-MB-P GeneOFF sirna Expression Kit (U6) 20 rxn PK-MB-P GeneOFF sirna Expression Kit (U6-GFP) 20 rxn PK-MB-P640300

75 4 Gene Cloning, Expression, and Silencing GeneOFF shrna Vector Kits Applications High-level expression of shrna for gene silencing Benefits Simple vector design and linear vector format for quick and convenient cloning G418 resistance gene for selection of stable cell lines Small vector size for efficient transfection Available with GFP reporter gene to determine transfection efficiency The GeneOFF shrna Vectors have been developed to efficiently induce RNA interference (RNAi) by expressing small hairpin RNAs (shrnas) in vitro or in vivo which are able to suppress distinct target genes. This is achieved by cloning an shrna coding sequence of nucleotides into an RNA polymerase III expression cassette. After transfection, the sirna sequence is expressed as a fold-back stem-loop structure that is subsequently processed in the cell into functional sirnas. The GeneOFF ppk-h1 and ppk-u6 shrna Vectors contain the optimized U6- or H1-RNA polymerase III promoters, allowing optimal expression in a wide variety of cell types. In addition, there are vector variants available which provide GFP reporter genes to allow easy tracking of vector transfection efficiency. All GeneOFF shrna Vectors contain a neomycin resistance gene for convenient selection of stable cell lines. In addition, two variants of these kits are available with a GFP reporter gene cassette to allow easy determination of the PCR fragment s transfection efficiency. For information about our sirna transfection reagents see chapter 5. Fig. 12: Plasmid maps of the four HSV TK Poly A puc ori Kan/Neo ppk-h1 (2921 bp) pamp/psv40 ph1 promoter Afl III BamHI sirna Cl EcoRI HSV TK Poly A Kan/Neo puc ori ppk-h1-gfp (5318 bp) pamp/psv40 pcmv ph1 GFP BamHI Poly A EcoRI sirna Cloning site NotI ppk shrna vectors without (left) and with (right) a GFP reporter gene. 4 Gene Cloning, Expression, and Silencing Afl III HSV TK Poly A puc ori pu6 promoter HSV TK Poly A ppk-u6 (3087 bp) BamHI sirna Cl ppk-u6-gfp (5318 bp) EcoRI Kan/Neo puc ori pcmv GFP Poly A EcoRI Kan/Neo pamp/psv40 pamp/psv40 pu6 BamHI sirna Cloning site NotI Product Size Catalog Number GeneOFF ppk-h1 shrna Vector Kit 25 rxn PK-MB-P GeneOFF ppk-h1-gfp shrna Vector Kit 25 rxn PK-MB-P GeneOFF ppk-u6 shrna Vector Kit 25 rxn PK-MB-P GeneOFF ppk-u6-gfp shrna Vector Kit 25 rxn PK-MB-P600300

76 74 4 Gene Cloning, Expression, and Silencing GeneOFF sirna Generation Kit 4 Gene Cloning, Expression, and Silencing Applications Easy and economic generation of functional sirnas Benefits Produces multiple sirna species thus greatly improving the success of silencing a target gene Many gene regions can be screened for silencing Kit includes the proven PromoFectin- sirna Transfection Reagent The PromoKine GeneOFF sirna Generation Kit provides easy and cost-effective generation of a large number of functional sirnas from full-length target genes in vitro. The GeneOFF sirna Genaration Kit combines rapid, high-yield synthesis of dsrna (using T7 in vitro transcription) and extremely complete cleavage of these dsrna templates into 22-bp sirnas by an ultra-active form of human Recombinant Dicer Enzyme. The kit contains sufficient reagents for generating a pool of small interfering RNAs (sirnas) from up to 5 different genes and for 50 transfections in 24-well plates. It includes a T7 Transcription Kit for 5 transcription reactions, 50 units of Recombinant Dicer Enzyme, RNA purification columns and PromoFectin-siRNA for 50 transfections (see information about PromoFectin-siRNA in chapter 5). PromoKine also provides an effective RNase inhbitor to protect sirna from degradation. siprotect is non-toxic to cells and very stable under a broad range of sirna transfection conditions. It is steril and free of DNases. Fig. 13: Principle of generating a pool of sirnas using the GeneOFF sirna Generation Kit. T7 promoter (Sense) T7 promoter (Sense) Sense RNA Target Sequence PCR T7 promoter (Anti-sense) Transcribe with T7 RNA polymerase Anneal and cleanup Anti-Sense RNA (Anti-sense) T7 promoter Digestion using recombinant Dicer TM enzyme and clean up Product Size Catalog Number GeneOFF sirna Generation Kit 1 kit PK-MB-T siprotect RNase Inhibitor 100 units PK-MB-T520100

77 Cell Transfection 5 The ability to introduce foreign nucleic acids into eukaryotic cells has become a powerful tool in studying and controlling gene expression. Cloned genes can be transfected into cells for biochemical characterization, mutational analyses, investigating gene regulatory elements, and producing specific proteins. Cell transfection promotes the rapid advancement of our knowledge of genetic regulation and protein function.

78 76 Contents Chapter 5 5 Cell Transfection 5.1 PromoFectin Transfection Reagents Magnet Assisted Transfection (MATra) Transfection Accessories... 85

79 5 Cell Transfection PromoFectin Transfection Reagents Applications Efficient transfection of many cell types including primary cells Suited for plasmids, oligonucleotides, sirna, and polypeptides For stable and transient transfections Benefits Superior transfection efficiency Fast procedure and easy handling without medium change Compatible with serum and antibiotics Good cell survival and viability after transfection PromoFectin PromoFectin is a series of reagents for highly efficient and reproducible transfection of a variety of adherent and non-adherent cells that can be used to transfect nucleic acids or proteins. PromoFectin comes either as a broad-range reagent or as specialized reagents for specific cell types and biomolecules. The standard, broad-range PromoFectin is a non-liposomal transfection reagent based on a water-soluble, cationic polymer. It is free of animal-derived components and shows very low cytotoxicity. PromoFectin ensures highly effective delivery of plasmids, oligonucleotides, and sirna into a variety of adherent and non-adherent cell types with a high degree of reproducibility. Since its development, PromoFectin has been used successfully with many problematic cell lines and sensitive primary cells. Fig. 1: Primary fibroblasts (NHDF cells, PromoCell) transfected with the GFP reporter vector ppk-cmv-r4 (see chapter 5) using PromoFectin. Nucleic acid Cytoplasm Nucleus Fig. 2: PromoFectin assembles nucleic acids into compact structures that bind to the cell surface and are delivered into the cell by endocytosis. In the endosome, PromoFectin acts as a proton sponge, buffering the endosomal ph that leads to ph inhibition of endosomal nucleases. Then, continuous proton influx leads to osmotic swelling and rupture of the endosome - allow- PromoFectin Cationic complexes Endocytosis Endosome Proton sponge ing for almost complete release of the nucleic acids into the cytosol. Moreover, PromoFectin also favours DNA trafficking to the cell nucleus a property that is particularly useful for trans- 5 Cell Transfection fecting slow-dividing or resting cells. Nucleic acid in 150 mm NaCl solution Fig. 3: The PromoFectin transfection procedure is fast and easy. Add PromoFectin to the nucleic acid solution, mix, incubate for 15 minutes, add growth medium (which can contain serum and antibiotics), and pipette PromoFectin Incubate 15 minutes at RT. Incubate h. Then, measure protein expression. the complexes onto the cells. The cells are then incubated for further analysis. No post-transfection removal of complexes or medium change/addition is necessary.

80 78 5 Cell Transfection Fig. 4: Comparison of transfection efficiencies for Promo- Fectin and three other common transfection reagents recommended for primary cells. Primary human fibroblasts (NHDF cells; PromoCell catalog number C-12300) cultured in 24-well plates were transfected with a luciferase reporter plasmid. The appropriate protocols recommended by the manufacturers were used for transfection with PromoFectin and the competing reagents. All transfections were performed in triplicate (values normalized to 1 mg protein). PromoFectin-HUVEC PromoFectin-Hepatocyte PromoFectin-Neuron PromoFectin-HUVEC is a non-liposomal PromoFectin-Hepatocyte is a ligand- PromoFectin-Neuron is a cationic, poly- PromoFectin derivative optimized for the conjugated, non-liposomal derivative of mer-based transfection reagent created transfection of primary endothelial cells PromoFectin specialized for hepatocyte for the transfection of primary neuronal such as HUVEC 1 and HDMEC 2. It shows transfection (e.g., primary human and cells, glial cells, and neuronal cell lines. 5 Cell Transfection very little cytotoxicity and enables very high transfection rates. murine hepatocytes, hepatocyte cell lines, and hepatocarcinoma cells). The reagent/nucleic acid complexes bind specifically to surface receptors on the target cells leading to extremely high Cells take up the polymer/dna complex through endocytosis, where the polymer is biodegraded into non-toxic molecules. Compared to viral methods, PromoFectin-Neuron allows less complicated and transfection rates. faster nucleic acid delivery with high transfection rates. Fig. 5: Primary human endothelial cells Fig. 6: Primary human hepatocytes Fig. 7: Primary neurons transfected with (HUVECs) transfected with the reporter transfected with the reporter vector the reporter vector ppk-cmv-r4 (see vector ppk-cmv-r4 (see chapter 4) ppk-cmv-r4 (see chapter 4) using chapter 4) using PromoFectin-Neuron. using PromoFectin-HUVEC. Nuclei PromoFectin-Hepatocyte. counterstained with DAPI. 1 Human Umbilical Vein Endothelial Cells 2 Human Dermal Microvascular Endothelial Cells

81 5 Cell Transfection 79 Fig. 8: Comparison of transfection efficiencies for PromoFectin-HUVEC, PromoFectin, and three other common transfection reagents recommended for primary cells. Primary HUVEC cells (PromoCell catalog number C-12200) cultured in 24-well plates were transfected with a luciferase reporter plasmid. The appropriate protocols recommended by the manufacturers were used for transfection with PromoFectin and the competing reagents. All transfections were performed in triplicate (values normalized to 1 mg protein). PromoFectin-Insect widely used cell lines (CHO, HeLa, COS, problematic cell lines and primary cells. It 293, NIH-3T3) at low costs. Its formula- enables optimal gene silencing with low PromoFectin-Insect is a unique, lipsome- tion and the provided protocol allow fast cytoxicity. based transfection reagent optimized for nucleic acid delivery into insect cells, especially those derived from Spodoptera and convenient cell transfection. PromoFectin-Oligo PromoFectin-Polypeptide frugiperda (e.g. Sf9 or Sf21). It outperforms other insect cell transfection reagents in comparison studies and requires less DNA. PromoFectin-Insect demonstrates broad vector delivery ca- PromoFectin-Oligo is a novel transfection reagent intended for the delivery of oligonucleotides into a wide variety of cell types. Unlike similar liposomal PromoFectin-Polypeptide is a novel liposomal reagent created for the highly efficient and fast delivery of proteins and polypeptides (e.g. blocking peptides and labeled antibodies) into a wide variety of 5 Cell Transfection pability, excellent reproducibility, and reagents, it shows low cytotoxicity and cells. It captures protein molecules and high expression levels for small- or large- excellent transfection efficiency. transports them into the cytosol of living scale protein production. PromoFectin-CellLine PromoFectin-siRNA cells while maintaining the protein s structure and functionality. This allows researchers to study intracellular protein PromoFectin-siRNA is a cationic, lipid- functions without the need for DNA PromoFectin-CellLine is a cationic, lipid- based transfection reagent designed for cloning and DNA transfection. based reagent developed to obtain op- the highly efficient delivery of sirna into PromoFectin-Polypeptide is also available timal transfection results with several a wide variety of cell types, including in pre-coated single-use tubes providing additional convenience and speed. Fig. 9: Left: NIH-3T3 cells transfected with the GFP reporter plasmid ppk-cmv-r4 (see chapter 4) using PromoFectin-Cell Line. Right: CHO cells transfected with the GFP reporter plasmid ppk-cmv-r4 (see chapter 4) using PromoFectin Cell Line.

82 80 5 Cell Transfection n Cells successfully transfected with PromoFectin Adipocyte cells Endothelial-like cells Epithelial-like cells Fibroblast-like cells Hepatocyte-like cells Keratinocytes Leukemia cells / lymphoblasts Myotubes / myoblasts / muscle cells Neuronal cells Chondrocytes Glioblastoma Primary human pre-adipocytes BBMC; BAEC; HLMEC*, HUVEC*, H-MVEC, primary HUVEC*, primary HDMEC* 2CSFMEo, 3LL, 56FHTE8o, 6CSFMEo, 9HTEo-, A549, AsPC-1, BxPC-3, CaCo2, Ca Ski, CFNPE90- CF, CFPEo, CFSMEo, CHO, D 407, DHD Pro.b, HeLa, Huh-7D12, IGROV1, MCF-7, MDA-MB435S, MDCK, MIAPaCa-2, MLE-12, MLE-15, MRC-5, Panc-1, PGHAM-1, SAOS-2, SiHa, SKOV3, U-2 OS BHK-21, COS-7, CV-1, HEK-293, L929, NIH 3T3, primary human skin fibroblasts (NHDF) BNL, CL 2**, HepG2**, Huh-7**, M-PAC, primary human and mouse hepatocytes** Primary human keratinocytes Daudi, Jurkat, K562, Molt 4, Melanoma, B16-F10 C2C12 CLBPEC, Neuro2A, PC12, SH-SY5Y, SHEP, SK-N-MC, primary chicken embryonic neurons HCS-2/8 U87 * PromoFectin-HUVEC, ** PromoFectin-Hepatocyte See for information about new cell types that have been successfully transfected using PromoFectin. Product Size Catalog Number 5 Cell Transfection PromoFectin *0.1 ml 0.5 ml 1 ml PromoFectin-HUVEC *** 0.1 ml 0.5 ml PromoFectin-Hepatocyte *** 0.1 ml 0.5 ml PromoFectin-Neuron ** 0.75 ml 5 x 0.75 ml PromoFectin-Insect *** 0.5 ml 1 ml PromoFectin-CellLine * 0.75 ml * 1.5 ml PromoFectin-Oligo ** 0.5 ml 1 ml 5 x 1 ml PromoFectin-siRNA 50 reactions 200 reactions 5 x 200 reactions PromoFectin-Polypeptide 24 reactions 24 Single Use Vials 96 reactions 96 Single Use Vials PK-CT PK-CT PK-CT PK-CT-2000-HUV-10 PK-CT-2000-HUV-50 PK-CT-2000-HEP-10 PK-CT-2000-HEP-50 PK-CT-2000-NEU-075 PK-CT-2000-NEU-375 PK-CT-2000-InC-50 PK-CT-2000-InC-100 PK-CT-2000-CL-075 PK-CT-2000-CL-150 PK-CT-2000-OLI-050 PK-CT-2000-OLI-100 PK-CT-2000-OLI-500 PK-CT-2000-RNA-50 PK-CT-2000-RNA-200 PK-CT-2000-RNA-1000 PK-CT-2000-POL-24 PK-CT-2000-POL-24Q PK-CT-2000-POL-96 PK-CT-2000-POL-96Q * 1 ml is sufficient to perform up to 500 transfections in 24-well plates. ** 1 ml is sufficient to perform up to 400 transfections in 24-well plates. *** 1 ml is sufficient to perform up to 300 transfections in 24-well plates.

83 5 Cell Transfection Magnet Assisted Transfection (MATra) Applications Magnet Assisted Transfection Magnet Assisted Lipofection Transfection of adherent and suspension cells including primary cells Suited for plasmids, oligonucleotides, sirna, and adenovirus/retrovirus transfection For stable and transient transfections Also suited for high-thoughput transfection Adherent Cells Suspension Cells Adherent Cells Suspension Cells MATra-A or MATra-si Reagent Use MATra-S Immobilizer to make the cells adherent. MA Lipofection Enhancer and lipofection reagent of choice Use MATra-S Immobilizer to make the cells adherent. Benefits Easy, fast, and reproducible procedure Functional with and without serum and antibiotics Very high transfection rates also with many hard-to-transfect cell types Up to several thousand fold increased transgene expression Very low amounts of nucleic acid necessary for transfection Magnet Assisted Transfection (MATra) is a very fast, easy-to-handle, and highly efficient technology for transfecting cells in culture. It can be used for cells from a broad variety of species. The major components of MATra are coated magnetic nanoparticles, which effectively bind either nucleic acids or liposomes. Using a special magnet plate, the nanoparticles with the bound nucleic acids or liposomes are rapidly drawn towards the cells and are deposited at a high concentration on the outer cell membrane. Afterwards, the cells endocytose the particles, which leads to a high transfection rate even with problematic cells. MATra nanoparticles DNA + nanoparticle complex DNA Adherent cells Transfected Cells Fig. 10: Magnet Assisted Transfection (MATra) principle. The MATra technology is distributed for IBA GmbH, Germany. For research use only! Two approaches are possible: Magnet Assisted Transfection using MATra-A or MATra-siRNA Reagent. Magnet Assisted Lipofection using the MA Lipofection Enhancer and a lipofection reagent of choice. Both techniques can be used with adherent cells as well as with suspension cells. For suspension cells, the cells have to be immobilized at the bottom of the wells to make use of the magnetic field. This can be achieved by either temporarily immobilizing the cells with MATra-S Immobilizer or by using modified culture vessels. MATra is also suited for highthroughput transfection assays using adapted protocols. Magnet Plate Magnet Transfected Cells MATra-A Reagent The MATra-A Reagent contains magnetic nanoparticles with an innovative polyelectrolyte coating, which effectively bind nucleic acids (e.g. plasmid DNA or oligonucleotides) by means of electrostatic interactions. Using this technique, nucleic acids are initially mixed with specifically coated magnetic nanoparticles. The magnetic particles loaded with the nucleic acids are then added to cells in culture and a strong magnetic force is applied beneath the cells. Exploiting this strong magnetic field, the full nucleic acid dose is rapidly drawn towards and delivered into the target cells on the bottom of the multi-well plate or cell culture flask. This results in very close contact between the cell membrane and the highly concentrated nucleic acid loaded magnetic complexes. MATra allows efficient transfection without disturbing the cell membrane architecture, causing chromosomal damage, or leaving a hole in the cell membrane as with other transfection technologies. The transfection rate consequently is clearly increased - also with difficult-to-transfect cells such as primary cells. In addition, the amounts of nucleic acids required for successful transfection can be significantly reduced, which in turn further reduces cytotoxicity. 5 Cell Transfection

84 82 5 Cell Transfection Phase contrast GFP fluorescence Overlay Fig. 11: Mouse fibrosarcoma cells transfected with GFP plasmid using MATra. (Data kindly provided for IBA GmbH by Dr. Lutz Thon and Dr. Dieter Adam, Institute for Immunology, University Schleswig-Holstein, Kiel, Germany). MATra-siRNA Reagent Specifically designed small inhibitory RNA oligonucleotides (sirnas) allow sirnas are small interfering/inhibitory transient inhibition of gene expression in sequence-specific RNA molecules that vitro and in vivo. bind to a complementary sequence MATra-siRNA contains specifically coat- of a target mrna, block translation of ed magnetic nanoparticles, which are that target mrna, and initiate its deg- optimized to effectively bind and stabi- radation. Consequently, synthesis of lize sirna. It provides optimal delivery the corresponding protein of interest is of sirna into many different cell types prevented and the specific gene function enabling efficient and prolonged gene- Fig. 12: Human endometrial stromal is knocked-out. This process is termed silencing. cells transfected with fluorescein-labeled RNA interference or gene-silencing and Please see chapter 4.4 for more gene sirna using MATra-siRNA Reagent. it is used to elucidate gene functions. silencing tools. (Data kindly provided for IBA GmbH by Dr. Birgit Gellersen, Endokrinologikum 5 Cell Transfection %GFP positive cells Hamburg, Germany) Fig. 13: Comparison of transfection efficiencies of MATra, PromoFectin-HUVEC, and three other common transfection reagents recommended for primary cells. Primary human dermal microvascular endothelial cells (HDMEC, PromoCell catalog number C-12210) cultured in 24-well plates were transfected with a GFP reporter plasmid. The appropriate protocols recommended by the manufacturers were used for transfection with MATra, PromoFectin, and the competing reagents. All transfections were performed in triplicate MATra PromoFectin-HUVEC Reagent FG6 Reagent S18 Reagent LF

85 5 Cell Transfection 83 MA Lipofection Enhancer The MA Lipofection Enhancer contains coated magnetic nanoparticles, which bind liposomes and any polycationic transfection reagents (e.g. PromoFectin) which in turn bind nucleic acids (e.g. plasmid DNA, oligonucleotides, sirna). Using the MA Lipofection Enhancer, cells are transfected more efficiently than with liposomal or polycationic transfection reagents alone. MATra-S Immobilizer Suspension cells have to be made adherent first to perform Magnet Assisted Transfection (as well as many other conventional transfection procedures). The MATra-S Immobilizer contains coated magnetic nanoparticles, which bind suspension cells. Using a special magnet plate, the magnetic nanoparticles with the bound cells are rapidly drawn towards the bottom of the cell culture vessel, where they are immobilized. By combining the MATra-S Immobilizer with the other MATra reagents, suspension cells can be transfected conveniently. Liposome Cell membrane Magnetic nanoparticles Fig. 14: Principle of Magnet Assisted Lipofection. The MA Lipofection Enhancer can be combined with any commercially available transfection reagent based on polycations or lipids. See for a listing of more than 100 successfully transfected cell types using Magnet Assisted Transfection and Magnet Assisted Lipofection. Response (expression level) MATra 100 Fig. 15: Magnet Assisted Transfection and lipofection in comparison. Luciferase expression was assayed after 24 hours. With MATra reagents, low vector doses are sufficient to obtain good results generation lipofection reagents 1. generation lipofection reagents Dose (µg DNA) 5 Cell Transfection %GFP positive cells 10 Fig. 16: Comparison of transfection efficiencies of two lipofection reagents with and without MA Lipofection Enhancer. Primary human dermal microvascular endothelial cells (HDMEC, PromoCell catalog number C-12210) cultured in 24-well plates were transfected with a GFP reporter plasmid. The appropriate protocols recommended by the manufacturers were used for transfection. All transfections were performed in triplicate. IBAfect liposomal transfection reagent is available at PromoKine on request. Please visit for more information IBAfect + MA Lipopfection Enhancer IBAfect Reagent LF + MA Lipopfection Enhancer Reagent LF

86 84 5 Cell Transfection Magnet Plates To perform Magnet Assisted Transfection, specially designed neodymium magnet plates are necessary. PromoKine offers them in different sizes, optimized for different cell culture vessels. Universal Magnet Plate (8 x 13 cm): suitable for 6-well, 12-well, 24-well, 48-well, or 96-well plates, T75 flasks, 60 mm dishes etc. Universal Magnet Plate (26 x 26 cm): suitable for 6-well, 12-well, 24-well, 48-well, or 96-well plates, T75 flasks, 60 mm dishes, 100 mm dishes etc. 24 Magnet Bar Plate (8 x 12 cm): optimized for 24-well plates. 96 Magnet Bar Plate (8 x 12 cm): optimized for 96-well plates Also available: Magnet Bar Plate Sets. Fig. 17: Universal magnet plate (8 x 13 cm) with collection of different culture plates and MATra reagents. Fig. 18: 96 magnet bar plate (8 x 12 cm) with 96-well culture plate. 5 Cell Transfection Product Name Size Catalog Number MATra-A Reagent * 50 µl 200 µl 1 ml MATra-siRNA Reagent * 50 µl 200 µl 1 ml MA Lipofection Enhancer 50 µl 200 µl 1 ml MATra-S Immobilizer ** 200 µl 1 ml Magnet Plate universal 8 x 13 cm universal 25 x 25 cm universal 26 x 26 cm for 24-well plates for 96-well plates Set of four Magnet Bar Plates Four 24 Magnet Bar Plates Four 96 Magnet Bar Plates Two 24 and two 96 Magnet Bar Plates PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT PK-CT * 1 µl is sufficient to transfect up to 1 µg nucleic acids. ** 1 µl is sufficient to immobilize up to 35,000 cells. With the MATra Evaluation Sets for adherent or suspension cells, you can test the MATra technology with your target cell type. Please see our website or contact us directly for more information on this special trial offer.

87 5 Cell Transfection Transfection Accessories GeneBooster Applications Enhancement of gene expression in transfected cells Benefits Up to 12 fold increased gene expression levels in diverse cell lines after transfection Compatible with all commonly used cell transfection reagents Fast and easy protocol Optimized conditions for popular cell lines Efficient for both transient and stable transfections The GeneBooster Reagents are chemical cocktails designed to significantly enhance gene expression levels in transfected cells. Three unique formulations have been developed for different cell types. Simply add GeneBooster reagent to the culture medium four hours after transfection and an increase in expression levels up to 12 fold can be achieved. The reagents can be used in combination with all commercially available DNA transfection reagents (including all PromoFectin Reagents). One vial of GeneBooster Reagent is sufficient for 150 transfections using 1 ml transfection volume. The three GeneBooster Reagents are also available in a kit. Fig. 19: K562, HeLa-S3, MCF7, and P19 cells were transfected with the beta-gal reporter plasmid ppk-cmv-r1 (see chapter 4). After 4 hours, the appropriate GeneBooster reagent was added to the cells which were stained 48 hours post transfection using a beta-gal Staining Kit (see chapter 1) and examined under a microscope Cell Clean Applications Highly efficient cleanup of transfected cells Benefits Efficient removal of extracellular lipid/ DNA complexes after cell transfection Non-toxic to cells Fast and easy procedure Better downstream results post transfection CellClean is a unique washing buffer that has been designed to remove all complexes of DNA and cationic lipids that associate with cell surfaces during transfection. It shows no cytotoxicity and can distinguish between extracellular and intracellular plasmid DNA. When Cell- Clean is used as part of the cell washing procedure, more precise quantitation of the amount of DNA delivered into cells can be achieved. Moreover, it improves downstream results such as DNA stability, cytometry and gene delivery studies. Fig. 20: Adherent cells were incubated with cationic lipid/dna complexes for 4 hours and washed with PBS (lane 1) or CellClean (lane 2). Cells were lysed, the DNA extracted and analysed by agarose gel electrophoresis. Arrows show extracellular plasmid DNA that could be removed from cell surfaces when using Cell Clean. 5 Cell Transfection Product Size Catalog Number GeneBooster Reagent I 3 vials x 1.5 ml each PK-CT GeneBooster Reagent II 3 vials x 1.5 ml each PK-CT GeneBooster Reagent III 3 vials x 1.5 ml each PK-CT GeneBooster Reagent Kit 3 vials x 1.5 ml each PK-CT CellClean 100 ml PK-CT

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89 Immunology 6 Antibodies have a broad range of applications like the detection of cellular structures as well as the specific identification, localization, and purification of intracellular and extracellular biomolecules such as proteins. They are also essential tools in describing cellular processes and for diagnostic purposes. Consequently, they are important for many research disciplines like apoptosis, signal transduction, neurobiology, virology and tumor biology.

90 88 Contents Chapter 6 6 Immunology 6.1 Mono- and Polyclonal Antibodies Labeled Antibodies and Labeling Kits ELISA Kits Antigens and Cell Lysates

91 Mono- and Polyclonal Antibodies PromoKine offers a wide and continuously growing range of mono- and polyclonal antibodies against a variety of interesting molecules like cytokines, growth factors and other important key targets in immunology, signal transduction, apoptosis, neurobiology, virology, and tumor biology. All antibodies are purified and highly active and can be used for diverse applications such as ELISAs, flow cytometry, functional studies, immunofluorescence microscopy, immunohistochemistry, immunoprecipitation or Westem blotting. For many of our antibodies, blocking peptides and tissue lysates are available that can be used for functional studies or as positive controls (see 5.3). Other antibodies are also available as conjugates with e.g. FITC or biotin. n Cell Signaling: Cytokines, Growth Factors, Receptors, and Ligands Product Name Product Description Size* Application** Catalog Number 2K antibody (mab) Mouse Anti-Human 2K 500 µg CS PK-AB BBL antibody Rabbit Anti-Human 4-1BB Ligand 100 µg WB, E PK-AB BBR antibody Rabbit Anti-Human 4-1BB Receptor 100 µg WB PK-AB BBR antibody Goat Anti-Human 4-1BB Receptor 100 µg WB, E PK-AB G 90K/Mac-2BP antibody (mab) Mouse Anti-Human 90K/Macrophage Antigen 2 Binding Protein (clone SP-2) 100 µg IHC (P), WB PK-AB K/Mac-2BP antibody (mab), FITC Mouse Anti-Human 90K/Macrophage Antigen 2 Binding Protein (clone SP-2; FITC-labeled) 100 tests not tested PK-AB F Adipolean Variant antibody Rabbit Anti-Human Adipolean Variant 100 µg WB, E PK-AB Adiponectin / Acrp30 antibody (mab) Adiponectin / Acrp30 antibody (mab) Adiponectin / Acrp30 (CT) antibody Adiponectin / Acrp30 (NT) antibody beta-actin antibody (mab) beta-actin (CT) antibody beta-actin (NT) antibody Mouse Anti-Human Adiponectin (Adipocyte Complement Related Protein of 30 kda; clone P1G12AT) Mouse Anti-Human/Mouse/Rat Adiponectin (Adipocyte Complement Related Protein of 30 kda; clone BV801) Rabbit Anti-Human/Mouse Adipokine (Adipocyte Complement Related Protein of 30 kda, CT) Rabbit Anti-Human/Mouse/Rat Adipokine (Adipocyte Complement Related Protein of 30 kda, NT) Mouse Anti-Human/Mouse/Rat/Pig/Sheep/Chicken/ Rabbit/Bovine beta-actin Rabbit Anti-Human/Mouse/Rat beta-actin (Major Cytoskeletal Protein, CT) Rabbit Anti-Human/Mouse/Rat beta-actin (Major Cytoskeletal Protein, NT) 50 µg E, WB PK-AB µg WB, E PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IF, IHC PK-AB µg WB, ICC PK-AB µg WB, IHC PK-AB beta-actin antibody Rabbit Anti-Human/Mouse beta-actin 100 µg WB PK-AB beta-actin antibody AID antibody Rabbit Anti-Human/Mouse/Rat/Monkey/Hamster beta-actin Rabbit Anti-Human/Mouse Activation Induced Cytidine Deaminase 100 µg WB, IP, IHC PK-AB µg WB PK-AB AITRL antibody Rabbit Anti-Human Activation Induced TNFR Member Ligand 100 µg WB, E PK-AB Ang-1 antibody (mab) Mouse Anti-Human Angiopoietin µg WB, IHC PK-AB Ang-2 antibody (mab) Mouse Anti-Human Angiopoietin µg WB PK-AB Apo A1 antibody Rabbit Anti-Human Apolipoprotein A1 100 µg WB PK-AB Apo B antibody Goat Anti-Human Apolipoprotein B 100 µg WB, IP, IHC, E PK-AB G Apo E3 antibody Rabbit Anti-Human Apo E3 100 µg WB, E PK-AB Apo-SAA antibody Rabbit Anti-Human Apolipoprotein Serum Amyloid A 100 µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

92 90 Product Name Product Description Size* Application** Catalog Number APRIL antibody Rabbit Anti-Human APRIL (Member of the TNF Family) 100 µg WB, E PK-AB APRIL (ED) antibody APRIL (ED2) antibody Rabbit Anti-Human APRIL (Member of the TNF Family) Rabbit Anti-Human/Mouse APRIL (Member of the TNF Family) 100 µg WB PK-AB µg WB, IHC PK-AB Artemin antibody Rabbit Anti-Human Artemin 100 µg WB, E PK-AB B220 antibody (mab) Rat Anti-Mouse B220 (B-cell specific isoform of CD45, CD45R) 500 µg FC PK-AB B-71 antibody Rabbit Anti-Mouse B µg WB, E PK-AB M BAFF antibody (mab) BAFF antibody BAFF (CT) antibody BAFF antibody BAFF-R / BR3 (CT) antibody Mouse Anti-Human B-cell Activating Factor in TNF Family (BLys) Rabbit Anti-Human B-cell Activating Factor in TNF Family (BLyS) Rabbit Anti-Human/Mouse/Rat B-cell Activating Factor in TNF Family (BLyS) Goat Anti-Human B-cell Activating Factor in TNF Family (BLyS) Rabbit Anti-Human/Mouse/Rat BAFF Receptor (BR3, CT) 500 µg E PK-AB µg WB PK-AB µg WB PK-AB µg WB, E PK-AB G 100 µg WB, IHC PK-AB BCA-1 antibody Rabbit Anti-Human B-cell Attracting Chemokine µg WB, E PK-AB BCMA antibody Rabbit Anti-Human B-cell Maturation Protein (Receptor for BAFF & APRIL) 100 µg WB PK-AB BD-1 antibody Rabbit Anti-Human beta-defensin µg WB, E PK-AB BD-2 antibody Goat Anti-Human beta-defensin µg WB, E PK-AB G BD-3 antibody Rabbit Anti-Human beta-defensin µg WB, E PK-AB BD-4 antibody Rabbit Anti-Human beta-defensin µg WB, E PK-AB BDNF antibody (mab) Mouse Anti-Human Brain Derived Neurotrophic Factor 500 µg WB, E, IP, IHC PK-AB BDNF antibody Rabbit Anti-Human Brain Derived Neurotrophic Factor 100 µg WB, E PK-AB BDNF antibody Goat Anti-Human Brain Derived Neurotrophic Factor 100 µg WB, E PK-AB G Betacellulin antibody Rabbit Anti-Human Betacellulin 100 µg WB, E PK-AB BLC antibody Rabbit Anti-Mouse B-Lymphocyte Chemoattractant 100 µg WB PK-AB BMP-1 antibody Rabbit Anti-Human/Mouse Bone Morphogenetic Protein µg WB PK-AB BMP-2 antibody (mab) Mouse Anti-Human Bone Morphogenetic Protein µg E PK-AB BMP-2 antibody Rabbit Anti-Human Bone Morphogenetic Protein µg WB, E PK-AB BMP-3 antibody Rabbit Anti-Human/Mouse/Rat Bone Morphogenetic Protein µg WB PK-AB BMP-3 antibody Goat Anti-Human Bone Morphogenetic Protein µg WB PK-AB G BMP-4 antibody (mab) Mouse Anti-Human Bone Morphogenetic Protein µg WB, E, N PK-AB BMP-4 antibody BMP-4 antibody BMP-5 antibody Rabbit Anti-Human/Mouse/Rat/Rabbit/Chicken Bone Morphogenetic Protein 4 Goat Anti-Human/Mouse/Rat Bone Morphogenetic Protein 4 Rabbit Anti-Human/Mouse Bone Morphogenetic Protein µg WB PK-AB µg WB, IP, IHC PK-AB G 100 µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

93 91 Product Name Product Description Size* Application** Catalog Number BMP-5 antibody Goat Anti-Human/Mouse/Rat Bone Morphogenetic Protein µg WB PK-AB G BMP-6 antibody Goat Anti-Human Bone Morphogenetic Protein µg WB PK-AB G BMP-7 antibody (mab) Mouse Anti-Human Bone Morphogenetic Protein µg E PK-AB BMP-7 antibody Rabbit Anti-Human Bone Morphogenetic Protein µg WB, E PK-AB BMP-7 antibody Goat Anti-Human/Mouse/Rat Bone Morphogenetic Protein µg WB PK-AB G BMP-8 antibody Goat Anti-Human Bone Morphogenetic Protein µg WB PK-AB G BMP-13 antibody Rabbit Anti-Human Bone Morphogenetic Protein µg WB PK-AB BMP-14 antibody Rabbit Anti-Human Bone Morphogenetic Protein µg WB PK-AB BRAK antibody Rabbit Anti-Human BRAK (CXCL14) 100 µg WB, E PK-AB BSA antibody Rabbit Anti-Bovine Serum Albumin 100 µg WB PK-AB C-10 antibody Rabbit Anti-Mouse C µg WB, E PK-AB M C3aR / CD88 antibody Rabbit Anti-Human C3a Receptor (CD88) 100 µg FC PK-AB C3aR antibody Rabbit Anti-Human C3a Receptor 100 µg FC PK-AB C5a antibody Rabbit Anti-Human C5a 100 µg WB PK-AB Cadherin antibody Rabbit Anti-Human/Mouse Cadherin 100 µg WB, IHC, IP PK-AB Cardiotrophin-1 (CT-1) antibody CCR1 antibody CCR3 (CT) antibody CCR3 (NT) antibody CCR4 antibody CCR5 (NT) antibody CCR8 (EL) antibody CCR10 antibody CD1A (T6, LEU6) antibody (mab) CD2 (T11, LFA-2) antibody (mab) Rabbit Anti-Human Cardiotrophin 1 (CT-1) 100 µg WB, E PK-AB Rabbit Anti-Human/Mouse/Monkey CC-Chemokine Receptor 1 Rabbit Anti-Human/Mouse/Rat CC-Chemokine Receptor 3 (HIV & Chemokine Receptor, CT) Rabbit Anti-Human/Mouse/Rat CC-Chemokine Receptor 3 (HIV & Chemokine Receptor, NT) Rabbit Anti-Human/Mouse/Rat CC-Chemokine Receptor 4 Rabbit Anti-Human CC-Chemokine Receptor 5 (HIV & Chemokine Receptor, NT) Rabbit Anti-Human CC-Chemokine Receptor 8 (HIV & Chemokine Receptor, EL) Rabbit Anti-Human/Mouse CC-Chemokine Receptor 10 (HIV & Chemokine Receptor) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB Mouse Anti-Human CD1A (T6, LEU6; clone hcd1a) 500 µg IS PK-AB Mouse Anti-Human CD2 (T11, LFA-2; clone hcd2) 500 µg FST (BL), IS PK-AB CD3 antibody (mab) Mouse Anti-Human CD3 500 µg FST (BL), FC, A CD3 antibody (mab), biotin CD3 antibody (mab), FITC Mouse Anti-Human CD3 (biotinylated) 500 µg FST (BL), FC, A Mouse Anti-Human CD3 (FITC-labeled) 500 µg FST (BL), FC, A PK-AB PK-AB PK-AB CD3 antibody (mab) Rat Anti-Mouse CD3 500 µg CS PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

94 92 Product Name Product Description Size* Application** Catalog Number CD4 antibody (mab) Mouse Anti-Human CD4 500 µg FST (BL), FC, A PK-AB CD4 antibody (mab), biotin CD4 antibody (mab), FITC Mouse Anti-Human CD4 (biotinylated) 500 µg FST (BL), FC, A Mouse Anti-Human CD4 (FITC-labeled) 500 µg FST (BL), FC, A PK-AB PK-AB CD4 antibody (mab) Rat Anti-Mouse CD4 500 µg CS PK-AB CD5 antibody (mab) Mouse Anti-Human CD5 500 µg FC PK-AB CD8 antibody (mab) Mouse Anti-Human CD8 500 µg FC PK-AB CD8 antibody (mab), biotin CD8 antibody (mab), FITC Mouse Anti-Human CD8 (biotinylated) 500 µg FC PK-AB Mouse Anti-Human CD8 (FITC-labeled) 500 µg FC PK-AB CD8a antibody (mab) Rat Anti-Mouse CD8a 200 µg IHC (P), FC PK-AB RM CD11a antibody (mab) Rat Anti-Mouse CD11a 500 µg FST (BL), FC PK-AB CD11b antibody (mab), FITC CD11c / p150,95 antibody (mab) Rat Anti-Mouse CD11b (FITC-labeled) 500 µg FST (BL), FC PK-AB Mouse Anti-Human CD11c (p150,95) 100 µg FC, FST (BL), IHC PK-AB CD11c antibody (mab) Rat Anti-Mouse CD11c 200 µg IHC (P), IP, FC PK-AB RM CD22L antibody Rabbit Anti-Human CD22 Ligand 100 µg WB, E PK-AB CD25 antibody (mab) Rat Anti-Mouse CD µg IHC (P), FC PK-AB RM CD26 antibody (mab) Mouse Anti-Human CD26 (clone M-A261) 100 µg FC, IHC, IP PK-AB CD26 antibody (mab), biotin CD26 antibody (mab), FITC Mouse Anti-Human CD26 (clone M-A261; biotinylated) Mouse Anti-Human CD26 (clone M-A261; FITC-labeled) 100 tests 100 tests FC FC PK-AB B PK-AB F CD27 antibody Goat Anti-Human/Mouse/Rat CD µg WB PK-AB G CD28 antibody (mab) Rat Anti-Mouse CD µg IHC (P), FC PK-AB RM CD31 antibody (mab) Rat Anti-Mouse CD µg IHC (P) PK-AB RM CD31 antibody (mab) Mouse Anti-Human CD31 (clone 158-2B3) 50 µg WB, IHC, IF, FC PK-AB CD34 antibody (mab) Rat Anti-Mouse CD µg IHC (P) PK-AB RM CD34 antibody (mab) Mouse Anti-Human CD34 (clone 34C02) 50 µg IHC, FC PK-AB CD40 antibody (mab) Mouse Anti-Human CD40 (clone 3B2) 100 µg E, WB, IHC PK-AB CD40 antibody Rabbit Anti-Human/Mouse/Rat/Monkey/Pig CD µg WB PK-AB CD40L antibody (mab) Hamster Anti-Mouse CD40 Ligand (clone MR1) 100 µg in vivo studies PK-AB HM CD40L antibody (mab) Mouse Anti-Human CD40 Ligand (clone MK13A4) 100 µg FST PK-AB CD40L antibody (mab), biotin CD40L antibody (mab), FITC Mouse Anti-Human CD40 Ligand (clone MK13A4; biotinylated) Mouse Anti-Human CD40 Ligand (clone MK13A4; FITC-labeled) 100 tests 100 tests not tested not tested PK-AB B PK-AB F CD40L antibody Goat Anti-Human CD40 Ligand 100 µg WB, E PK-AB G CD40L antibody Rabbit Anti-Human CD40 Ligand 100 µg WB, E PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

95 93 Product Name Product Description Size* Application** Catalog Number CD40L antibody Rabbit Anti-Human/Mouse/Rat CD40 Ligand 100 µg WB, E PK-AB CD44std antibody (mab) Mouse Anti-Human CD44 (standard; clone SFF-2) 100 µg FC, IHC PK-AB CD44std antibody (mab), biotin Mouse Anti-Human CD44 (standard; clone SFF-2; biotinylated) 100 tests FC PK-AB B CD44std antibody (mab), FITC Mouse Anti-Human CD44 (standard; clone SFF-2; FITC-labeled) 100 tests FC PK-AB F CD44std antibody (mab) Mouse Anti-Human CD44 (standard; clone SFF304) 100 µg FC, WB, IHC (P) PK-AB CD44std antibody (mab), biotin Mouse Anti-Human CD44 (standard; clone SFF304; biotinylated) 100 tests FC PK-AB B CD44std antibody (mab), FITC Mouse Anti-Human CD44 (standard; clone SFF304; FITC-labeled) 100 tests FC PK-AB F CD44var (v3) antibody (mab) Mouse Anti-Human CD44 (variant 3; clone VFF-327) 100 µg FC, WB, IHC (P) PK-AB CD44var (v3) antibody (mab), biotin Mouse Anti-Human CD44 (variant 3; clone VFF-327; biotinylated) 100 tests FC PK-AB B CD44var (v4) antibody (mab) Mouse Anti-Human CD44 (variant 4, clone VFF-11) 100 µg IHC PK-AB CD44var (v4) antibody (mab), FITC Mouse Anti-Human CD44 (variant 4, clone VFF-11; FITC-labeled) 100 tests not tested PK-AB F CD44var (v4) antibody (mab) Rat Anti-Mouse CD44 (variant 4, clone 10D1) 100 µg E, WB, IHC (P) PK-AB RM CD44var (v5) antibody (mab) Mouse Anti-Human CD44 (variant 5; clone VFF8) 100 µg FC, WB, IHC (P) PK-AB CD44var (v5) antibody (mab), biotin Mouse Anti-Human CD44 (variant 5; clone VFF8; biotinylated) 100 tests FC PK-AB B CD44var (v6) antibody (mab) Mouse Anti-Human CD44 (variant 6, clone VFF-7) 100 µg FC, WB, IHC (P) PK-AB CD44var (v6) antibody (mab), biotin Mouse Anti-Human CD44 (variant 6, clone VFF-7; biotinylated) 100 tests FC PK-AB B CD44var (v6) antibody (mab), FITC Mouse Anti-Human CD44 (variant 6, clone VFF-7; FITC-labeled) 100 tests FC PK-AB F CD44var (v6) antibody (mab) CD44var (v6) antibody (mab), biotin CD44var (v6) antibody (mab), FITC Mouse Anti-Human CD44 (variant 6, clone VFF-18) Mouse Anti-Human CD44 (variant 6, clone VFF-18; biotinylated) Mouse Anti-Human CD44 (variant 6, clone VFF-18; FITC-labeled) 100 µg FC, WB, IHC (P) 100 tests 100 tests FC FC PK-AB PK-AB B PK-AB F CD44var (v6) antibody (mab) Rat Anti-Mouse CD44 (variant 6; clone 9A4) 100 µg E, WB, IHC PK-AB RM CD44var (v7) antibody (mab) Mouse Anti-Human CD44 (variant 7; clone VFF-9) 100 µg FC, WB PK-AB CD44var (v7) antibody (mab), FITC Mouse Anti-Human CD44 (variant 7; clone VFF-9; FITC-labeled) 100 tests FC PK-AB F CD44var (v7-v8) antibody (mab) Mouse Anti-Human CD44 (variant 7-8; clone VFF-17) 100 µg WB PK-AB CD44var (v7-v8) antibody (mab), biotin Mouse Anti-Human CD44 (variant 7-8; clone VFF-17; biotinylated) 100 tests not tested PK-AB B Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

96 94 Product Name Product Description Size* Application** Catalog Number CD44var (v7-v8) antibody (mab), FITC CD44var (v10) antibody (mab) CD44var (v3-v10) antibody Mouse Anti-Human CD44 (variant 7-8; clone VFF-17; FITC-labeled) 100 tests not tested PK-AB F Mouse Anti-Human CD44 (variant 10; clone VFF-14) 100 µg WB PK-AB Goat Anti-Human CD44 (variant 3-10) 1 mg WB, IHC (P) PK- AB CD62E antibody (mab) Mouse Anti-Human E-selectin (clone hcd62e) 500 µg IS PK-AB CD80 / B7-1 antibody (mab) CD86 / B7-2 antibody (mab) CD90 (Thy-1) antibody (mab) CD90 (Thy-1.1) antibody (mab) CD90 (Thy-1.2) antibody (mab) scd105 (Endoglin) antibody (mab) scd105 (Endoglin) antibody (mab) scd105 / Endoglin antibody scd105 / Endoglin antibody Rat Anti-Mouse CD80 (B7-1) 500 µg FST (BL), FC PK-AB Rat Anti-Mouse CD86 (B7-2) 200 µg IHC (P), FC PK-AB RM Rat Anti-Mouse CD90 (Thymus Cell Antigen 1) 500 µg CS PK-AB Rat Anti-Mouse CD90 (Thymus Cell Antigen 1.1) 500 µg CS PK-AB Rat Anti-Mouse CD90 (Thymus Cell Antigen 1.2) 500 µg CS PK-AB Mouse Anti-Human soluble CD105 (Endoglin; clone MJ7/18) 100 µg WB, IHC, IP, FC PK-AB Rat Anti-Mouse soluble CD105 (Endoglin) 100 µg E PK-AB RM Rabbit Anti-Human scd105 (Endoglin) 100 µg WB, E PK-AB PC Rabbit Anti-Mouse scd105 (Endoglin) 100 µg WB, E PK-AB MPC CNTF antibody Rabbit Anti-Human Ciliary Neurotrophic Factor 100 µg WB, E PK-AB CNTF antibody Rabbit Anti-Rat Ciliary Neurotrophic Factor 100 µg WB, E PK-AB R CRTH2 (IN) antibody CRTH2 (NT) antibody CTGF antibody (mab) CTGF antibody Rabbit Anti-Human/Mouse/Rat CRTH2 (Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells, IN) Rabbit Anti-Human/Mouse/Rat CRTH2 (Chemoattractant Receptor-homologous Molecule Expressed on Th2 Cells, NT) Mouse Anti-Human Connective Tissue Growth Factor (clone 6B13) Rabbit Anti-Human/Mouse/Rat Connective Tissue Growth Factor 100 µg WB, IHC PK-AB µg WB, IHC PK-AB µg IHC, E PK-AB µg WB, IHC PK-AB CTGF antibody Rabbit Anti-Human Connective Tissue Growth Factor 100 µg WB, E PK-AB CTGFL / WISP-2 antibody CTLA-4 antibody CTRP1 / GIP (IN) antibody CTRP1 / GIP (NT) antibody CTRP2 (IN) antibody Rabbit Anti-Human Connective Tissue Growth Factor- Like Protein (WISP-2) Rabbit Anti-Mouse Cytotoxic T-lymphocyte Associated Antigen 4 Rabbit Anti-Human/Mouse C1q/TNF-alpha Related Protein 1 (Adipokine-related, IN) Rabbit Anti-Human/Mouse/Rat C1q/TNF-alpha Related Protein 1 (Adipokine-related, NT) Rabbit Anti-Human/Mouse C1q/TNF-alpha Related Protein 2 (Adipokine-related, IN) 100 µg WB, E PK-AB µg WB, E PK-AB M 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

97 95 Product Name Product Description Size* Application** Catalog Number CTRP2 (NT) antibody CTRP2 antibody CTRP3 / CORS26 (IN) antibody CTRP4 (CT) antibody CTRP4 (NT) antibody CTRP5 (IN) antibody CTRP5 (NT) antibody CTRP6 (CT) antibody CTRP6 (IN) antibody CTRP7 (CT) antibody CTRP7 (NT) antibody CTRP7 antibody CX3CR1 / V28 (EL) antibody CX3CR1 / V28 (NT) antibody Rabbit Anti-Human/Mouse C1q/TNF-alpha Related Protein 2 (Adipokine-related, NT) Rabbit Anti-Human/Mouse/Rat C1q/TNF-alpha Related Protein 2 (Adipokine-related) Rabbit Anti-Human/Mouse C1q/TNF-alpha Related Protein 3 (Adipokine-related, CORS26, IN) Rabbit Anti-Human/Mouse/Rat C1q/TNF-alpha Related Protein 4 (Adipokine-related, CT) Rabbit Anti-Human C1q/TNF-alpha Related Protein 4 (Adipokine-related, NT) Rabbit Anti-Human/Mouse/Rat C1q/TNF-alpha Related Protein 5 (Adipokine-related, IN) Rabbit Anti-Human/Rat C1q/TNF-alpha Related Protein 5 (Adipokine-related, NT) Rabbit Anti-Human/Mouse C1q/TNF-alpha Related Protein 6 (Adipokine-related, CT) Rabbit Anti-Human/Mouse/Rat C1q/TNF-alpha Related Protein 6 (Adipokine-related, IN) Rabbit Anti-Human/Mouse/Rat C1q/TNF-alpha Related Protein 7 (Adipokine-related;CT) Rabbit Anti-Human/Mouse/Rat C1q/TNF-alpha Related Protein 7 (Adipokine-related, NT) Rabbit Anti-Human/Mouse/Rat C1q/TNF-alpha Related Protein 7 (Adipokine-related) Rabbit Anti-Human CX3C-Chemokine Receptor 1 (HIV & Chemokine Receptor, V28, EL) Rabbit Anti-Human/Mouse/Rat CX3C-Chemokine Receptor 1 (HIV & Chemokine Receptor, V28, NT) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC, FC PK-AB CXCL16 antibody Rabbit Anti-Human CXC Ligand µg WB, E PK-AB CXCL16 antibody Goat Anti-Mouse CXC Ligand µg WB, E PK-AB GM CXCR4 (EL) antibody CXCR4 (NT) antibody Rabbit Anti-Human/Mouse CXC-Chemokine Receptor 4 (HIV & Chemokine Receptor, EL) Rabbit Anti-Human/Mouse CXC-Chemokine Receptor 4 (HIV & Chemokine Receptor, NT) 100 µg WB PK-AB µg WB, IHC PK-AB CXCR4 antibody Rabbit Anti-Mouse/Rat CXC-Chemokine Receptor µg WB, FC, N PK-AB CXCR5 antibody Rabbit Anti-Human CXC-Chemokine Receptor µg WB PK-AB Cyr61 antibody Rabbit Anti-Human/Mouse/Rat Cysteine-rich Angiogenic Inducer µg WB PK-AB DAP12 antibody Rabbit Anti-Human DAP12 (KARAP) 100 µg WB PK-AB DAP12 antibody Rabbit Anti-Rat DAP12 (KARAP) 100 µg WB PK-AB E-cadherin antibody (mab) Mouse Anti-Human E-cadherin (Clone 21E9) 100 µg WB, IHC (P) PK-AB EGF antibody (mab) Mouse Anti-Human Epidermal Growth Factor 500 µg WB, E, IP PK-AB EGF antibody Rabbit Anti-Human Epidermal Growth Factor 100 µg WB, E PK-AB EGF antibody Goat Anti-Mouse Epidermal Growth Factor 100 µg WB, E PK-AB GM EGFR antibody Rabbit Anti-Human/Mouse Epidermal Growth Factor Receptor 100 µg WB, IP PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

98 96 Product Name Product Description Size* Application** Catalog Number EG-VEGF antibody Goat Anti-Human Vascular Endothelial Growth Factor-EG 100 µg WB, E PK-AB G EG-VEGF antibody EMAP-II antibody EMAP-II antibody EMAP-II antibody ENA-78 antibody Rabbit Anti-Human Vascular Endothelial Cell Growth Factor-EG Goat Anti-Human Endothelial/Monocyte Activating Polypeptide II Rabbit Anti-Human Endothelial/Monocyte Activating Polypeptide II Rabbit Anti-Mouse Endothelial/Monocyte Activating Polypeptide II Rabbit Anti-Human Epithelial Neutrophil Activating Peptide µg WB PK-AB µg WB, E PK-AB G 100 µg E PK-AB µg WB, E PK-AB M 100 µg WB, E PK-AB Endostatin antibody Rabbit Anti-Human Endostatin 100 µg WB, E PK-AB Eotaxin antibody Rabbit Anti-Human Eotaxin (CCR3 Ligand) 100 µg WB PK-AB Eotaxin (CT) antibody Rabbit Anti-Human Eotaxin (CCR3 Ligand) 100 µg WB PK-AB Eotaxin antibody Rabbit Anti-Mouse Eotaxin (CCR3 Ligand) 100 µg WB PK-AB Eotaxin-1 antibody (mab) Mouse Anti-Human Eotaxin µg WB, E, IP PK-AB Eotaxin-2 antibody (mab) Mouse Anti-Human Eotaxin µg WB, E, IP PK-AB Eotaxin-2 antibody Goat Anti-Mouse Eotaxin µg WB, E PK-AB GM Eotaxin-2 antibody Goat Anti-Human Eotaxin µg WB, E PK-AB G Eotaxin-3 antibody (mab) Mouse Anti-Human Eotaxin 3 (CCL26) 500 µg E, FST (BL) PK-AB Eotaxin-3 antibody Goat Anti-Human Eotaxin 3 (CCL26) 100 µg WB, E PK-AB G EPO antibody (mab) Mouse Anti-Human Erythropoietin 50 µg WB, E PK-AB EPO antibody (mab) Mouse Anti-Human Erythropoietin (clone NYRhEPO) 500 µg WB, E, IP PK-AB EPO-Receptor antibody (mab) Mouse Anti-Human EPO Receptor 100 µg WB, IHC (P) PK-AB E-selectin antibody (mab) E-selectin antibody (mab), biotin E-selectin antibody (mab), FITC Mouse Anti-Human E-selectin 100 µg E, FC, FST (BL), IHC (P) Mouse Anti-Human E-selectin (biotinylated) 100 tests Mouse Anti-Human E-selectin (FITC-labeled) 100 tests FC FC PK-AB PK-AB B PK-AB F E-selectin antibody (mab) Rat Anti-Mouse E-selectin 200 µg IHC (P), FC PK-AB RM E-selectin antibody Rabbit Anti-Human/Mouse/Rat E-selectin 100 µg WB, IP, IHC PK-AB Exodus-2 antibody Rabbit Anti-Human Exodus µg WB, E PK-AB Exodus-2 antibody Rabbit Anti-Mouse Exodus µg WB, E PK-AB M FasL / Apo1L antibody FGF-a / FGF-1 antibody FGF-b / FGF-2 antibody (mab) FGF-b / FGF-2 antibody Goat Anti-Human Fas Ligand (Apo1L) 100 µg WB, E PK-AB G Rabbit Anti-Human acidic Fibroblast Growth Factor (FGF-1) Mouse Anti-Human basic Fibroblast Growth Factor (FGF-2) Rabbit Anti-Human basic Fibroblast Growth Factor (FGF-2) 100 µg WB, E PK-AB µg E PK-AB µg WB, E PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

99 97 Product Name Product Description Size* Application** Catalog Number FGF-4 antibody Rabbit Anti-Human Fibroblast Growth Factor µg WB, E PK-AB FGF-4 antibody Rabbit Anti-Human/Mouse Fibroblast Growth Factor µg WB PK-AB FGF-5 antibody (mab) Mouse Anti-Human Fibroblast Growth Factor µg E PK-AB FGF-9 antibody Rabbit Anti-Mouse Fibroblast Growth Factor µg WB, E PK-AB M FGF-10 antibody Rabbit Anti-Human Fibroblast Growth Factor µg WB PK-AB FGF-10 antibody Goat Anti-Human Fibroblast Growth Factor µg WB, E PK-AB G FGF-16 antibody Goat Anti-Human Fibroblast Growth Factor µg WB, E PK-AB G FGF-17 antibody Rabbit Anti-Human Fibroblast Growth Factor µg WB, E PK-AB FGF-17 antibody Goat Anti-Human Fibroblast Growth Factor µg WB, E PK-AB G FGF-19 antibody Goat Anti-Human Fibroblast Growth Factor µg WB, E, N PK-AB G Fibronectin antibody Rabbit Anti-Human Fibronectin 100 µg WB, E, IHC PK-AB flt-3 Ligand antibody Rabbit Anti-Human Fms-Related Tyrosine Kinase 3 Ligand 100 µg WB, E PK-AB Follistatin antibody Rabbit Anti-Human Follistatin 100 µg WB, E PK-AB Fractalkine antibody Rabbit Anti-Human Fractalkine 100 µg WB, E PK-AB Fractalkine antibody gacrp30 / Adipolean antibody Rabbit Anti-Human/Mouse/Rat Fractalkine 100 µg WB, IP, IHC PK-AB Goat Anti-Human globular Acrp30 (Adipolean) 100 µg WB, E PK-AB G Galectin-1 antibody Rabbit Anti-Human Galectin µg WB, E PK-AB Galectin-3 antibody Rabbit Anti-Human Galectin µg WB, E PK-AB Galectin-7 antibody Rabbit Anti-Human Galectin µg WB PK-AB Galectin-9 antibody Rabbit Anti-Human Galectin µg WB PK-AB GCP-2 antibody Rabbit Anti-Human Granulocyte Chemotactic Protein µg WB, E PK-AB G-CSF antibody (mab) Mouse Anti-Human Granulocyte Colony Stimulating Factor 500 µg WB, E, IP PK-AB G-CSF antibody Rabbit Anti-Human Granulocyte Colony Stimulating Factor 100 µg WB, E PK-AB G-CSF antibody Rabbit Anti-Mouse Granulocyte Colony Stimulating Factor 100 µg WB, E PK-AB M GDF-3 antibody Rabbit Anti-Human Growth & Differentiation Factor µg WB, E PK-AB GDF-9 / BMP15 antibody Rabbit Anti-Human Growth & Differentiation Factor 9 (BMP-15) 100 µg WB PK-AB GDNF antibody Rabbit Anti-Human Glial-Derived Neurotrophic Factor 100 µg WB, E PK-AB GDNF antibody GFRα-1 / GDNFRα (IN) antibody GFRα-2 / GDNFRβ (IN) antibody GFRα-3 / GDNFRγ (IN) antibody GH (Growth Hormone) antibody (mab) GH (Growth Hormone) antibody Rabbit Anti-Human/Mouse/Rat Glial Derived Neurotropic Factor Rabbit Anti-Human/Mouse/Rat GDNF Receptor alpha-1 (IN) Rabbit Anti-Human/Mouse/Rat GDNF Receptor alpha-2 (IN) Rabbit Anti-Human/Mouse/Rat GDNF Receptor alpha-3 (IN) 100 µg WB, IP, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Mouse Anti-Human Growth Hormone 500 µg WB, E, IP PK-AB Rabbit Anti-Human Growth Hormone 100 µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

100 98 Product Name Product Description Size* Application** Catalog Number Ghrelin (CT) antibody Ghrelin (CT) antibody Ghrelin (NT) antibody Rabbit Anti-Human Ghrelin (CT) 100 µg not tested PK-AB Rabbit Anti-Human Ghrelin (CT) 100 µg not tested PK-AB Rabbit Anti-Human Ghrelin (NT) 100 µg not tested PK-AB GITRL / AITRL antibody GM-CSF antibody (mab) GM-CSF antibody (mab) GM-CSF antibody GM-CSF antibody GRO / KC antibody GRO / KC antibody GRO / KC antibody GRO-alpha / MGSA antibody GRO-beta antibody GRO-beta / MIP-2 antibody GRO-gamma antibody Rabbit Anti-Human/Mouse Glucocorticoid Induced TNF Receptor Ligand (AITRL) Mouse Anti-Human Granulocyte/Macrophage Colony Stimulating Factor Rat Anti-Mouse Granulocyte/Macrophage Colony Stimulating Factor Rabbit Anti-Human Granulocyte/Macrophage Colony Stimulating Factor Rabbit Anti-Mouse Granulocyte/Macrophage Colony Stimulating Factor Rabbit Anti-Mouse Growth Regulated Oncogen (KC, CXCL1) Rabbit Anti-Mouse/Rat Growth Regulated Oncogen (KC, CXCL1) Rabbit Anti-Rat Growth Regulated Oncogen (KC, CXCL1) Rabbit Anti-Human Growth Regulated Oncogen alpha (CXCL1) Rabbit Anti-Human Growth Regulated Oncogen beta (CXCL2) Rabbit Anti-Rat Growth Regulated Oncogen beta (MIP-2, CXCL2) Rabbit Anti-Human Growth Regulated Oncogen gamma (CXCL3) 100 µg WB PK-AB µg WB, E, IP PK-AB µg E, WB, IHC PK-AB RM 100 µg WB, E PK-AB µg WB, E PK-AB M 100 µg WB, E PK-AB M 100 µg WB, IP, IHC, N PK-AB µg WB, E PK-AB R 100 µg WB, E PK-AB µg WB, E PK-AB µg WB, E PK-AB R 100 µg WB, E PK-AB HCC-1 antibody Rabbit Anti-Human Hemofiltrate CC Chemokine µg WB, E PK-AB HGF antibody Rabbit Anti-Human Hepatocyte Growth Factor 100 µg WB, E PK-AB I-309 antibody Rabbit Anti-Human I µg WB, E PK-AB ICAM-1 / CD54 antibody (mab) ICAM-1 / CD54 antibody (mab), biotin ICAM-1 / CD54 antibody (mab), FITC ICAM-1/ CD54 (NT) antibody (mab) ICAM-1 / CD 54 antibody (mab) ICAM-1 / CD 54 antibody (mab) ICAM-1 / CD 54 antibody Mouse Anti-Human Intracellular Adhesion Molecule 1 (clone RR1/1) Mouse Anti-Human Intracellular Adhesion Molecule 1 (clone RR1/1; biotinylated) Mouse Anti-Human Intracellular Adhesion Molecule 1 (clone RR1/1; FITC-labeled) Mouse Anti-Human Intracellular Adhesion Molecule 1 (NT) 50 µg E, WB, FC, FST (BL), IHC 100 tests 100 tests FC FC PK-AB PK-AB B PK-AB F 100 µg WB PK-AB Mouse Anti-Human ICAM-1 (CD54; clone 1H19) 100 µg WB, E PK-AB Rat Anti-Mouse ICAM-1 (CD54) 200 µg WB, IHC PK-AB RM Rabbit Anti-Human Intracellular Adhesion Molecule µg WB, IP, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

101 99 Product Name Product Description Size* Application** Catalog Number ICAM-2 / CD102 antibody (mab) ICAM-2 / CD102 antibody (mab), biotin ICAM-2 / CD102 antibody (mab), FITC ICAM-3 / CD50 antibody (mab) ICAM-3 / CD50 antibody (mab), biotin ICAM-3 / CD50 antibody (mab), FITC Mouse Anti-Human Intracellular Adhesion Molecule 2 (CD102; clone CBR-IC 2/2) Mouse Anti-Human Intracellular Adhesion Molecule 2 (CD102; clone CBR-IC 2/2; biotinylated) Mouse Anti-Human Intracellular Adhesion Molecule 2 (CD102; clone CBR-IC 2/2; FITC-labeled) Mouse Anti-Human Intracellular Adhesion Molecule 3 (CD50; clone CBR-IC3/1) Mouse Anti-Human Intracellular Adhesion Molecule 3 (CD50; clone CBR-IC3/1; biotinylated) Mouse Anti-Human Intracellular Adhesion Molecule 3 (CD50; clone CBR-IC3/1; FITC-labeled) 50 µg E, FC, FST (BL), IHC 100 tests 100 tests FC FC 50 µg E, FC, FST (BL), IHC 100 tests 100 tests FC FC PK-AB PK-AB B PK-AB F PK-AB PK-AB B PK-AB F IFN-alpha antibody (mab) Mouse Anti-Human Interferon alpha 500 µg WB, E, IP, IHC PK-AB IFN-alpha antibody (mab) Mouse Anti-Human Interferon alpha (clone EBI-1) 100 µg E, FST (BL) PK-AB IFN-alpha antibody Goat Anti-Human Interferon alpha 100 µg WB, E PK-AB G IFN-alpha antibody Rabbit Anti-Human Interferon alpha 100 µg WB, E, N PK-AB IFN-alpha antibody Rabbit Anti-Mouse/Rat Interferon alpha 100 µg WB, N PK-AB IFN-beta antibody (mab) Mouse Anti-Human Interferon beta 500 µg WB, E, IP, IHC PK-AB IFN-beta antibody Rabbit Anti-Human Interferon beta 100 µg WB, E PK-AB IFN-beta (IN) antibody Rabbit Anti-Human/Mouse Interferon-beta (IN) 100 µg WB, IHC PK-AB IFN-gamma antibody (mab) IFN-gamma antibody (mab) IFN-gamma antibody (mab) IFN-gamma antibody (mab) IFN-gamma antibody (mab) IFN-gamma antibody (mab), biotin IFN-gamma antibody (mab), FITC IFN-gamma antibody IFN-gamma antibody IFN-gamma antibody IFN-lambda-2 antibody IFN-omega antibody (mab) IGF BP-3 antibody Mouse Anti-Human Interferon gamma 500 µg WB, E, IP, IHC, IS Mouse Anti-Rat Interferon gamma (clone DB-1) 100 µg E, IHC, FST (BL) PK-AB PK-AB MR Rat Anti-Mouse Interferon gamma 500 µg WB, E, IP, IS PK-AB Rat Anti-Mouse Interferon gamma (clone F3 IGH 48) 100 µg E, bioactivity PK-AB RM Mouse Anti-Human Interferon gamma (clone GZ-4) 200 µg FST (BL) PK-AB Mouse Anti-Human Interferon gamma (clone GZ-4; biotinylated) Mouse Anti-Human Interferon gamma (clone GZ-4; FITC-labeled) 100 tests 100 tests not tested FC (IS) PK-AB B PK-AB F Goat Anti-Rat Interferon gamma 100 µg WB, E PK-AB GR Rabbit Anti-Human Interferon gamma 100 µg WB, E PK-AB Rabbit Anti-Mouse Interferon gamma 100 µg WB, E PK-AB M Rabbit Anti-Human Interferon lambda µg WB, E PK-AB Mouse Anti-Human Interferon omega (clone OMG-4) 100 µg E, FST (BL) PK-AB Rabbit Anti-Human Insulin-Like Growth Factor Binding Protein µg WB, E PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

102 100 Product Name Product Description Size* Application** Catalog Number IGF BP-5 antibody IGF BP-7 antibody Rabbit Anti-Human Insulin-Like Growth Factor Binding Protein 5 Rabbit Anti-Human Insulin-Like Growth Factor Binding Protein µg WB, E PK-AB µg WB, E PK-AB IGF-I antibody Goat Anti-Mouse Insulin-Like Growth Factor I 100 µg WB, E PK-AB GM IGF-I antibody Rabbit Anti-Human Insulin-Like Growth Factor I 100 µg WB, E PK-AB IGF-I antibody Rabbit Anti-Mouse Insulin-Like Growth Factor I 100 µg WB PK-AB IGF-II antibody Rabbit Anti-Human Insulin-Like Growth Factor II 100 µg WB, E PK-AB IL-1 alpha antibody (mab) Mouse Anti-Mouse Interleukin 1-alpha (clone Flo-1) 100 µg E, FST (BL) PK-AB RM IL-1 alpha antibody Rabbit Anti-Mouse Interleukin 1-alpha 100 µg WB, E PK-AB M IL-1 alpha antibody Rabbit Anti-Human Interleukin 1-alpha 100 µg WB, E PK-AB IL-1 alpha antibody Goat Anti-Rat Interleukin 1-alpha 100 µg WB, E PK-AB GR IL-1 alpha R1 antibody (mab) IL-1 alpha R2 antibody (mab) IL-1RAcP (C1) antibody IL-1RAcP (C2) antibody Rat Anti-Mouse Interleukin 1-alpha Receptor I (Clone 11B7) 100 µg WB PK-AB RM Rat Anti-Mouse Interleukin 1-alpha Receptor II 100 µg WB PK-AB RM Rabbit Anti-Human IL-1 Receptor Accessory Protein 100 µg WB PK-AB Rabbit Anti-Human IL-1 Receptor Accessory Protein 100 µg WB PK-AB IL-1 beta antibody (mab) Mouse Anti-Human Interleukin 1-beta 500 µg WB, E, IP, IHC PK-AB IL-1 beta antibody (mab) IL-1 beta antibody (mab), FITC Mouse Anti-Human Interleukin 1-beta (clone B-A15) 100 µg FST (BL) PK-AB Mouse Anti-Human Interleukin 1-beta (clone B-A15; FITC-labeled) 100 tests FC (IS) PK-AB F IL-1 beta antibody Rabbit Anti-Mouse Interleukin 1-beta 100 µg WB, E PK-AB M IL-1 beta antibody Rabbit Anti-Rat Interleukin 1-beta 100 µg WB, E PK-AB R IL-2 antibody (mab) Mouse Anti-Human Interleukin µg WB, E, IP, IHC, FST (BL) PK-AB IL-2 antibody (mab) Mouse Anti-Human Interleukin 2 (clone B-G5) 100 µg FST (BL) PK-AB IL-2 antibody (mab) Rat Anti-Mouse Interleukin µg E, FST (BL) PK-AB IL-2 antibody (mab) Rat Anti-Mouse Interleukin 2 (clone JES6-5H4) 100 µg E, WB, FC, IHC, IP PK-AB RM IL-2 antibody Goat Anti-Human Interleukin µg WB, E PK-AB G IL-2 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-2 antibody Rabbit Anti-Mouse Interleukin µg WB, E PK-AB M IL-2R antibody (mab) Mouse Anti-Human Interleukin 2 Receptor (CD25) 500 µg FST (BL), FC PK-AB IL-2R antibody (mab) Mouse Anti-Human Interleukin 2 Receptor (clone B-B10) 100 µg FC, FST (BL) PK-AB IL-2R antibody (mab) Rat Anti-Mouse Interleukin 2 Receptor 500 µg FST (BL) PK-AB IL-3 antibody (mab) Mouse Anti-Human Interleukin µg WB, E, IP, IHC PK-AB IL-3 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-3 antibody Rabbit Anti-Mouse Interleukin µg WB, E PK-AB M Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

103 101 Product Name Product Description Size* Application** Catalog Number IL-3 beta antibody Goat Anti-Rat Interleukin 3-beta 100 µg WB, E PK-AB GR IL-4 antibody (mab) Mouse Anti-Human Interleukin µg WB, E, IP, IHC PK-AB IL-4 antibody (mab) Mouse Anti-Human Interleukin 4 (clone B-S4) 100 µg FST (BL) PK-AB IL-4 antibody (mab), FITC Mouse Anti-Human Interleukin 4 (clone B-S4; FITC-labeled) IL-4 antibody (mab) Rat Anti-Mouse Interleukin µg WB, E, IP, IHC, IS 100 tests FC (IS) PK-AB F PK-AB IL-4 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-4 antibody Rabbit Anti-Mouse Interleukin µg WB, E PK-AB M IL-4 antibody Rabbit Anti-Rat Interleukin µg WB, E PK-AB R IL-5 antibody (mab) Rat Anti-Mouse Interleukin 5 (clone TRFK5) 100 µg E, FC (IS), FST (BL) PK-AB RM IL-5 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-6 antibody (mab) Mouse Anti-Human Interleukin µg WB, E, IP, IHC PK-AB IL-6 antibody (mab) Mouse Anti-Human Interleukin 6 (clone B-E8) 100 tests FST (BL) PK-AB IL-6 antibody (mab), FITC Mouse Anti-Human Interleukin 6 (clone B-E8; FITC-labeled) 100 tests FC (IS) PK-AB F IL-6 antibody (mab) Rat Anti-Human Interleukin 6 (clone I6YOR5/66) 100 µg E PK-AB R IL-6 antibody (mab) Rat Anti-Mouse Interleukin 6 (clone 6B4 IGH 54) 100 µg E, FST (BL) PK-AB RM IL-6 antibody Goat Anti-Human Interleukin µg WB, E PK-AB G IL-6 antibody Goat Anti-Rat Interleukin µg WB, E PK-AB GR IL-6 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-6 antibody Rabbit Anti-Mouse Interleukin µg WB, E PK-AB M IL-6 antibody Rabbit Anti-Rat Interleukin µg WB, E PK-AB R IL-6R antibody (mab) Mouse Anti-Human Interleukin 6 Receptor (clone B-R6) 100 µg FC, FST (BL) PK-AB IL-6R antibody (mab), biotin Mouse Anti-Human Interleukin 6 Receptor (clone B-R6; biotinylated) IL-7 antibody (mab) Mouse Anti-Human Interleukin µg WB, E, IP, IHC, IS 100 tests FC PK-AB B PK-AB IL-7 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-7 antibody Rabbit Anti-Mouse Interleukin µg WB, E PK-AB M IL-8 antibody (mab) Mouse Anti-Human Interleukin µg WB, E, IP, IHC PK-AB IL-8 antibody (mab) Mouse Anti-Human Interleukin 8 (clone NAP-II) 100 µg not tested PK-AB IL-8 antibody (mab), FITC Mouse Anti-Human Interleukin 8 (clone NAP-II; FITC-labeled) 100 tests FC (IS) PK-AB F IL-8 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-9 antibody Rabbit Anti-Mouse Interleukin µg WB, E PK-AB M IL-9 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-10 antibody (mab) Mouse Anti-Human Interleukin µg WB, E, IP, IHC, IS PK-AB IL-10 antibody (mab) Mouse Anti-Human Interleukin 10 (clone BT-10) 100 µg E PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

104 102 Product Name Product Description Size* Application** Catalog Number IL-10 antibody (mab), FITC Mouse Anti-Human Interleukin 10 (clone BT-10; FITC-labeled) 100 tests not tested IL-10 antibody (mab) Rat Anti-Mouse Interleukin µg WB, E, IP, IHC, IS PK-AB F PK-AB IL-10 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-10 antibody Rabbit Anti-Mouse Interleukin µg WB, E PK-AB M IL-10 antibody Rabbit Anti-Rat Interleukin µg WB, E PK-AB R IL-11 antibody (mab) Mouse Anti-Human Interleukin µg E, FST (BL) PK-AB IL-11 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-12 antibody (mab) Mouse Anti-Human Interleukin µg E PK-AB IL-12 antibody (mab) Mouse Anti-Human Interleukin 12 (clone B-T21) 100 µg FST (BL) PK-AB IL-12 antibody (mab) Rat Anti-Mouse Interleukin µg E PK-AB RM IL-12 antibody Goat Anti-Human Interleukin µg WB, E PK-AB G IL-12 antibody Goat Anti-Mouse Interleukin µg WB, E PK-AB GM IL-12-p40 antibody (mab) Rat Anti-Mouse Interleukin 12 (p40) 500 µg WB, E, IP PK-AB IL-12-p75 antibody (mab) Rat Anti-Mouse Interleukin 12 (p75; clone NYRmIL-12p75) 500 µg WB, E, IP PK-AB IL-13 antibody (mab) Mouse Anti-Human Interleukin 13 (clone B-P6) 100 µg FST (BL) PK-AB IL-13 antibody (mab), FITC Mouse Anti-Human Interleukin 13 (clone B-P6; FITC-labeled) 100 tests not tested PK-AB F IL-13 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-13 antibody Rabbit Anti-Mouse Interleukin µg WB, E PK-AB M IL-15 antibody (mab) Mouse Anti-Human Interleukin µg WB, E, IP, IHC PK-AB IL-15 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-15 antibody Rabbit Anti-Mouse Interleukin µg WB, E PK-AB M IL-16 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-17 antibody Goat Anti-Human Interleukin 17A 100 µg WB, E PK-AB G IL-17 antibody Rabbit Anti-Human Interleukin 17A 100 µg WB, E PK-AB IL-17 antibody Rabbit Anti-Mouse Interleukin 17A 100 µg WB, E PK-AB M IL-17B antibody Rabbit Anti-Human Interleukin 17B 100 µg WB, E PK-AB IL-17E antibody Rabbit Anti-Human Interleukin 17E 100 µg WB, E PK-AB IL-17F antibody Rabbit Anti-Human Interleukin 17F 100 µg WB, E PK-AB IL-18 antibody Rabbit Anti-Human Interleukin µg WB PK-AB IL-18 antibody Rabbit Anti-Mouse Interleukin µg WB, IP, N PK-AB IL-18 BP antibody Goat Anti-Mouse Interleukin 18 Binding Protein 100 µg WB, E PK-AB GM IL-19 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-20 antibody Goat Anti-Human Interleukin µg WB, E PK-AB G IL-21 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-21 (IN) antibody Rabbit Anti-Human Interleukin 21 (IN) 100 µg WB, IHC PK-AB IL-21R (ED) antibody Rabbit Anti-Human/Mouse/Rat Interleukin 21 Receptor (ED) 100 µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

105 103 Product Name Product Description Size* Application** Catalog Number IL-21R (NT) antibody Rabbit Anti-Human/Mouse/Rat Interleukin 21 Receptor (NT) 100 µg WB PK-AB IL-22 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-22 antibody Rabbit Anti-Mouse Interleukin µg WB, E PK-AB M IL-22R (CT) antibody IL-22R (NT) antibody Rabbit Anti-Human/Rat Interleukin 22 Receptor (CT) 100 µg WB PK-AB Rabbit Anti-Human/Rat Interleukin 22 Receptor (NT) 100 µg WB, IHC PK-AB IL-23 (CT) antibody Rabbit Anti-Human/Mouse Interleukin 23 (CT) 100 µg WB,IHC PK-AB IL-23 (NT) antibody Rabbit Anti-Human/Mouse Interleukin 23 (NT) 100 µg WB PK-AB IL-25 / SF20 antibody Rabbit Anti-Mouse Interleukin 25 (SF20) 100 µg WB, E PK-AB M IL-27 (IN) antibody Rabbit Anti-Human/Mouse Interleukin 27 (IN) 100 µg WB PK-AB IL-27 (NT) antibody Rabbit Anti-Human/Mouse Interleukin 27 (NT) 100 µg WB PK-AB IL-31 (IN) antibody Rabbit Anti-Human/Mouse Interleukin 31 (IN) 100 µg WB PK-AB IL-31 (NT) antibody Rabbit Anti-Mouse Interleukin 31 (NT) 100 µg WB,IHC PK-AB IL-31 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB IL-32 (CT) antibody Rabbit Anti-Human/Mouse Interleukin 32 (CT) 100 µg WB PK-AB IL-32 (IN) antibody Rabbit Anti-Human/Mouse Interleukin 32 (IN) 100 µg WB PK-AB IL-33 (IN) antibody Rabbit Anti-Human/Mouse/Rat Interleukin-33 (IN) 100 µg WB PK-AB IL-33 (NT) antibody Rabbit Anti-Human Interleukin-33 (NT) 100 µg WB, IF PK-AB IL-33 antibody Rabbit Anti-Human Interleukin µg WB, E PK-AB Insulin antibody (mab) Mouse Anti-Human Insulin (clones: C7C9, D4B8, 7F8, 3A6, 8E2, 7F5) 200 µg E, IHC PK-AB Insulin antibody Rabbit Anti-Human Insulin 100 µg WB PK-AB IP-10 antibody Rabbit Anti-Human IFN-gamma Inducible Protein µg WB, E PK-AB IP-10 antibody Rabbit Anti-Mouse IFN-gamma Inducible Protein µg WB, E PK-AB M IP-10 antibody Rabbit Anti-Rat IFN-gamma Inducible Protein µg WB PK-AB IRF3 (CT) antibody Rabbit Anti-Human/Mouse Interferon Regulatory Factor 3 (CT) 100 µg WB, IHC PK-AB IRF3 (IN) antibody Rabbit Anti-Human Interferon Regulatory Factor 3 (IN) 100 µg WB PK-AB IRF7 (CT) antibody Rabbit Anti-Human/Mouse/Rat Interferon Regulatory Factor 7 (CT) 100 µg WB PK-AB IRF7 (IN) antibody Rabbit Anti-Human Interferon Regulatory Factor 7 (IN) 100 µg WB PK-AB IRF8 antibody I-TAC antibody Rabbit Anti-Human/Mouse/Rat Interferon Regulatory Factor 8 Rabbit Anti-Human IFN Inducible T-cell alpha Chemoattractant 100 µg WB PK-AB µg WB, E PK-AB KGF antibody Rabbit Anti-Human Keratinocyte Growth Factor 100 µg WB, E PK-AB Lano antibody Rabbit Anti-Human Lano (Member of the LAP Family) 100 µg WB PK-AB LARC antibody Rabbit Anti-Rat Liver & Activation Regulated Chemokine 100 µg WB, IP PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

106 104 Product Name Product Description Size* Application** Catalog Number LD78-beta antibody Goat Anti-Human LD78 beta 100 µg WB, E PK-AB G LEC antibody (mab) LEC antibody Mouse Anti-Human Liver Expressed Chemokine (NCC-4, CCL16) Goat Anti-Human Liver Expressed Chemokine (NCC-4, CCL16) 500 µg E PK-AB µg WB, E PK-AB G Leptin antibody (mab) Mouse Anti-Human Leptin 500 µg WB, E, IP PK-AB Leptin antibody Goat Anti-Rat Leptin 100 µg WB, E PK-AB GR Leptin antibody Rabbit Anti-Human Leptin 100 µg WB, E PK-AB Leptin antibody Rabbit Anti-Mouse Leptin 100 µg WB, E PK-AB M Leptin Receptor antibody LFA-1 alpha / CD11a antibody (mab) LFA-1 alpha / CD11a antibody (mab), biotin LFA-1 alpha / CD11a antibody (mab), FITC LFA-1 beta / CD18 antibody (mab) LFA-1 beta / CD18 antibody (mab), biotin LFA-1 beta / CD18 antibody (mab), FITC Rabbit Anti-Mouse Leptin Receptor 100 µg WB PK-AB Mouse Anti-Human Lymphocyte Function Associated Antigen 1-alpha (CD11a; clone R7.1) Mouse Anti-Human Lymphocyte Function Associated Antigen 1-alpha (CD11a; clone R7.1; biotinylated) Mouse Anti-Human Lymphocyte Function Associated Antigen 1-alpha (CD11a; clone R7.1; FITC-labeled) Mouse Anti-Human Lymphocyte Function Associated Antigen 1-beta (CD18; clone R3.3) Mouse Anti-Human Lymphocyte Function Associated Antigen 1-beta (CD18; clone R3.3; biotinylated) Mouse Anti-Human Lymphocyte Function Associated Antigen 1-beta (CD18; clone R3.3; FITC-labeled) 100 µg FC, FST (BL), IHC 100 tests 100 tests FC FC 100 µg FC, FST (BL), IHC 100 tests 100 tests FC FC PK-AB PK-AB B PK-AB F PK-AB PK-AB B PK-AB F LIGHT antibody Rabbit Anti-Human LIGHT (Member of the TNF Family) 100 µg WB, E PK-AB LIX antibody Rabbit Anti-Mouse Lipopolysaccharide Induced CXC Chemokine (CXCL5) 100 µg WB, E PK-AB M L-selectin antibody (mab) Mouse Anti-Human L-selectin (clone DREGG.55) 100 µg E, FC, IHC PK-AB L-selectin antibody (mab), biotin L-selectin antibody (mab), FITC Mouse Anti-Human L-selectin (DREGG.55; biotinylated) Mouse Anti-Human L-selectin (DREGG.55; FITC-labeled) 100 tests 100 tests FC FC PK-AB B PK-AB F L-selectin antibody (mab) Rat Anti-Mouse L-selectin (clone 12D6) 200 µg IHC (P), FC PK-AB RM L-selectin antibody Rabbit Anti-Human L-selectin (CD62L) 100 µg WB, IP, IHC PK-AB Lymphotactin antibody Lymphotactin antibody Lymphotactin antibody Rabbit Anti-Rat Lymphotactin 100 µg WB, IP, N PK-AB Rabbit Anti-Mouse Lymphotactin 100 µg WB, IP PK-AB Rabbit Anti-Human Lymphotactin 100 µg WB, E PK-AB slyve-1 antibody (mab) Mouse Anti-Human slyve µg WB PK-AB slyve-1 antibody (mab) Rat Anti-Mouse slyve-1 (clone 4D17) 200 µg WB, IHC PK-AB RM slyve-1 antibody Rabbit Anti-Mouse slyve µg WB, E, IHC, IF PK-AB MPC slyve-1 antibody Rabbit Anti-Human slyve µg WB, E, IHC, IF PK-AB PC Mac-1 alpha / CD11b antibody (mab) Mouse Anti-Human Macrophage Antigen 1-alpha (CD11b; clone LM2/1) 100 µg FC, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

107 105 Product Name Product Description Size* Application** Catalog Number Mac-1 alpha / CD11b antibody (mab), biotin Mac-1 alpha / CD11b antibody (mab), FITC MCAF / MCP-1 antibody (mab) Mouse Anti-Human Macrophage Antigen 1-alpha (CD11b; clone LM2/1; biotinylated) Mouse Anti-Human Macrophage Antigen 1-alpha (CD11b; clone LM2/1; FITC-labeled) Mouse Anti-Human Monocyte Chemotactic & Activating Factor (MCP-1) MCP-1 antibody Rabbit Anti-Human Monocyte Chemotactic Protein 1 (JE, MCAF) MCP-1 antibody Rabbit Anti-Mouse Monocyte Chemotactic Protein 1 (JE, MCAF) MCP-1 antibody Rabbit Anti-Mouse/Rat Monocyte Chemotactic Protein 1 (JE, MCAF) MCP-1 antibody Rabbit Anti-Rat Monocyte Chemotactic Protein 1 (JE, MCAF) 100 tests 100 tests FC FC PK-AB B PK-AB F 500 µg WB, E, IP PK-AB µg WB, E PK-AB µg WB, E PK-AB M 100 µg WB, N PK-AB µg WB, E PK-AB R MCP-2 antibody (mab) Mouse Anti-Human Monocyte Chemotactic Protein µg E PK-AB MCP-2 antibody Rabbit Anti-Human Monocyte Chemotactic Protein µg WB, E PK-AB MCP-2 antibody Rabbit Anti-Mouse Monocyte Chemotactic Protein µg WB, E PK-AB M MCP-3 antibody (mab) Mouse Anti-Human Monocyte Chemotactic Protein µg WB, E, IP PK-AB MCP-3 antibody Goat Anti-Human Monocyte Chemotactic Protein µg WB, E PK-AB G MCP-3 antibody Goat Anti-Mouse Monocyte Chemotactic Protein µg WB, E PK-AB GM MCP-3 antibody Rabbit Anti-Human Monocyte Chemotactic Protein µg WB, E PK-AB MCP-4 antibody (mab) Mouse Anti-Human Monocyte Chemotactic Protein µg E, FST (BL) PK-AB MCP-4 antibody Goat Anti-Mouse Monocyte Chemotactic Protein µg WB, E, N PK-AB G MCP-4 antibody Rabbit Anti-Human Monocyte Chemotactic Protein µg WB, E PK-AB MCP-5 antibody Rabbit Anti-Mouse Monocyte Chemotactic Protein µg WB, E PK-AB M M-CSF antibody Rabbit Anti-Human Macrophage Colony Stimulating Factor 100 µg WB, E PK-AB M-CSF antibody Goat Anti-Mouse Macrophage Colony Stimulating Factor 100 µg WB, E PK-AB GM MD-1 (CT) antibody Rabbit Anti-Human MD-1 (Lymphocyte Antigen 86) 100 µg WB PK-AB MDC antibody (mab) Mouse Anti-Human Macrophage Derived Chemokine 500 µg E PK-AB MDC antibody Rabbit Anti-Human Macrophage Derived Chemokine 100 µg WB, E PK-AB MDC antibody Rabbit Anti-Mouse Macrophage Derived Chemokine 100 µg WB, E PK-AB M MDC antibody Rabbit Anti-Mouse/Rat Macrophage Derived Chemokine 100 µg WB PK-AB MIA antibody Rabbit Anti-Human Melanoma Inhibitory Activity Protein 100 µg WB, E PK-AB MIA-2 antibody Rabbit Anti-Human Melanoma Inhibitory Activity Protein µg WB, E PK-AB Midkine antibody Rabbit Anti-Human Midkine 100 µg WB, E PK-AB MIF antibody Rabbit Anti-Mouse/Rat Mesoderm Inducing Factor 100 µg WB, IP PK-AB MIG antibody (mab) MIG antibody MIP-1 alpha antibody (mab) Mouse Anti-Human Monokine Induced by IFN gamma (CXCL9) Rabbit Anti-Human Monokine Induced by IFN gamma (CXCL9) Mouse Anti-Human Macrophage Inflammatory Protein 1-alpha (CCL3) 500 µg E PK-AB µg WB, E PK-AB µg WB, E, IP PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

108 106 Product Name Product Description Size* Application** Catalog Number MIP-1 alpha antibody MIP-1 alpha antibody MIP-1 alpha antibody MIP-1 alpha antibody MIP-1 beta antibody MIP-1 beta antibody MIP-1 beta antibody MIP-1 gamma antibody Goat Anti-Human Macrophage Inflammatory Protein 1-alpha Rabbit Anti-Mouse Macrophage Inflammatory Protein 1-alpha Rabbit Anti-Mouse/Rat Macrophage Inflammatory Protein 1-alpha Rabbit Anti-Rat Macrophage Inflammatory Protein 1-alpha Rabbit Anti-Human Macrophage Inflammatory Protein 1-beta Rabbit Anti-Mouse Macrophage Inflammatory Protein 1-beta Rabbit Anti-Mouse/Rat Macrophage Inflammatory Protein 1-beta Rabbit Anti-Mouse Macrophage Inflammatory Protein 1-gamma 100 µg WB, E PK-AB G 100 µg WB, E PK-AB M 100 µg WB, IP PK-AB µg WB, E PK-AB R 100 µg WB, E PK-AB µg WB, E PK-AB M 100 µg WB, IP, N PK-AB µg WB, E PK-AB M MIP-2 antibody Rabbit Anti-Mouse Macrophage Inflammatory Protein µg WB, E PK-AB M MIP-2 antibody MIP-3 antibody (mab) MIP-3 alpha antibody (mab) Rabbit Anti-Mouse/Rat Macrophage Inflammatory Protein 2 Mouse Anti-Human Macrophage Inflammatory Protein 3 (CCL20) Mouse Anti-Human Macrophage Inflammatory Protein 3-alpha (CCL20; clone YNR-HMIP3a) 100 µg WB, IP, N PK-AB µg WB, E, IP PK-AB µg E PK-AB MIP-3 antibody Rabbit Anti-Human Macrophage Inflammatory Protein µg WB, E PK-AB MIP-3 alpha antibody MIP-3 beta antibody (mab) MIP-3 beta antibody MIP-4 antibody Rabbit Anti-Human Macrophage Inflammatory Protein 3-alpha Mouse Anti-Human Macrophage Inflammatory Protein 3-beta (CCL19; clone YNR-HMIP3b) Rabbit Anti-Human Macrophage Inflammatory Protein 3-beta Rabbit Anti-Human Macrophage Inflammatory Protein µg WB, E PK-AB µg E PK-AB µg WB, E PK-AB µg WB, E PK-AB MIP-5 antibody Goat Anti-Human Macrophage Inflammatory Protein µg WB, E PK-AB G MMP-2 antibody Rabbit Anti-Human/Mouse/Rat Matrix Metalloproteinase µg WB, IP, IHC PK-AB MMP-2 antibody Rabbit Anti-Rat Matrix Metalloproteinase µg WB, IP PK-AB MMP-3 antibody (mab) Mouse Anti-Human Matrix Metalloproteinase µg WB, IHC PK-AB MMP-3 antibody Rabbit Anti-Human/Mouse/Rat/Horse/Rabbit Matrix Metalloproteinase µg WB PK-AB MMP-3 antibody Goat Anti-Human/Mouse/Rat Matrix Metalloproteinase µg WB, IP, IHC PK-AB G MMP-7 antibody (mab) Mouse Anti-Human Matrix Metalloproteinase µg WB, IHC PK-AB MMP-8 antibody (mab) Mouse Anti-Human Matrix Metalloproteinase µg WB, IHC PK-AB MMP-8 antibody Goat Anti-Human/Mouse/Rat Matrix Metalloproteinase µg WB, IP, IHC PK-AB G MMP-9 antibody Rabbit Anti-Human Matrix Metalloproteinase µg WB PK-AB MMP-9 antibody Rabbit Anti-Rat Matrix Metalloproteinase µg WB, IP, IHC PK-AB MMP-10 antibody (mab) Mouse Anti-Human Matrix Metalloproteinase µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

109 107 Product Name Product Description Size* Application** Catalog Number MMP-11 antibody Rabbit Anti-Human/Mouse/Rat Matrix Metalloproteinase µg WB, IP, IHC PK-AB MMP-12 antibody (mab) Mouse Anti-Human Matrix Metalloproteinase µg WB, IHC PK-AB MMP-12 antibody Rabbit Anti-Human/Mouse Matrix Metalloproteinase µg WB PK-AB MMP-12 antibody Rabbit Anti-Human Matrix Metalloproteinase µg WB, IP, IHC PK-AB MMP-13 antibody (mab) Mouse Anti-Human Matrix Metalloproteinase µg WB, IHC PK-AB MMP-13 antibody Rabbit Anti-Human/Mouse/Rat Matrix Metalloproteinase µg WB, IP, IHC PK-AB MMP-14 antibody (mab) Mouse Anti-Human Matrix Metalloproteinase µg WB, IHC PK-AB MMP-15 antibody (mab) Mouse Anti-Human Matrix Metalloproteinase µg WB, IHC PK-AB MMP-17 antibody Rabbit Anti-Human/Mouse/Rat Matrix Metalloproteinase µg WB, IP, IHC PK-AB MMP-19 antibody Rabbit Anti-Human Matrix Metalloproteinase µg WB, IP, IHC PK-AB MMP-24 antibody Rabbit Anti-Human/Mouse Matrix Metalloproteinase µg WB, IP, IHC PK-AB MMP-25 antibody (mab) Mouse Anti-Human Matrix Metalloproteinase µg WB, IHC PK-AB Mpg-1 antibody Myeloperoxidase (MPO) antibody Rabbit Anti-Mouse/Rat Macrophage-specific Gene (MSG, MPS-1) 100 µg WB PK-AB Rabbit Anti-Human/Mouse Myeloperoxidase 100 µg WB PK-AB Nanog antibody Rabbit Anti-Human Nanog 100 µg WB, E PK-AB NAP-2 antibody (mab) Mouse Anti-Human Neutrophil Activating Protein µg WB, E, IP PK-AB NAP-2 antibody Rabbit Anti-Human Neutrophil Activating Protein µg WB, E PK-AB NAP-2 antibody Goat Anti-Human Neutrophil Activating Protein µg WB, E PK-AB G Neuroserpin antibody Rabbit Anti-Human Neuroserpin 100 µg WB, E PK-AB Neurturin antibody Rabbit Anti-Human Neurturin (Ligand for GFRa-2) 100 µg WB, E PK-AB Neurturin antibody Rabbit Anti-Human Neurturin (Ligand for GFRa-2) 100 µg WB PK-AB Neutrophil antibody (mab) Rat Anti-Mouse Neutrophil 200 µg WB, IHC (P) PK-AB RM beta-ngf antibody (mab) Mouse Anti-Human Nerve Growth Factor beta 500 µg E PK-AB beta-ngf antibody Rabbit Anti-Human Nerve Growth Factor beta 100 µg WB, E PK-AB NGFR / TNFRSF16 antibody NNT-1 / BCSF-3 antibody Rabbit Anti-Human/Mouse Nerve Growth Factor Receptor (TNFRSF16) 100 µg WB PK-AB Rabbit Anti-Human Novel Neurotrophin-1 (BCSF-3) 100 µg WB, E PK-AB NOV antibody Rabbit Anti-Human Nephroblastoma Overexpressed Gene 100 µg WB, E PK-AB NP-1 antibody Goat Anti-Human Neuropilin µg WB, E PK-AB G NT-3 antibody Rabbit Anti-Human Neurotrophin µg WB, E PK-AB NT-3 antibody Goat Anti-Human Neurotrophin µg WB, E PK-AB G NT-4 antibody (mab) Mouse Anti-Human Neurotrophin µg WB, E, IP PK-AB NT-4 antibody Rabbit Anti-Human Neurotrophin µg WB, E PK-AB NT-4 antibody Goat Anti-Human Neurotrophin µg WB, E PK-AB G Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

110 108 Product Name Product Description Size* Application** Catalog Number Oncostatin M antibody Rabbit Anti-Human Oncostatin M 100 µg WB, E PK-AB OPG antibody Rabbit Anti-Human Osteoprotegerin 100 µg WB, E PK-AB PAI-1 antibody Rabbit Anti-Human Plasminogen Activator Inhibitor µg WB, E PK-AB PAI-1 antibody Rabbit Anti-Rat Plasminogen Activator Inhibitor µg WB PK-AB PDGF-AA antibody (mab) Mouse Anti-Human Platelet Derived Growth Factor AA 100 µg WB PK-AB PDGF-AA antibody Rabbit Anti-Human Platelet Derived Growth Factor AA 100 µg WB, E PK-AB PDGF-BB antibody (mab) Mouse Anti-Human Platelet Derived Growth Factor BB 100 µg WB PK-AB PDGF-BB antibody Rabbit Anti-Human Platelet Derived Growth Factor BB 100 µg WB, E PK-AB PDGFR-alpha antibody (mab) PDGFR-alpha antibody (mab) PDGFR-beta antibody (mab) PDGFR-beta antibody (mab) PECAM-1 antibody (mab) PECAM-1 antibody (mab), biotin PECAM-1 antibody (mab), FITC Mouse Anti-Human Platelet Derived Growth Factor Receptor alpha Rat Anti-Mouse Platelet Derived Growth Factor Receptor alpha Mouse Anti-Human Platelet Derived Growth Factor Receptor beta Rat Anti-Mouse Platelet Derived Growth Factor Receptor beta Mouse Anti-Human Platelet Endothelial Cell Adhesion Molecule 1 (clone Gi18) Mouse Anti-Human Platelet Endothelial Cell Adhesion Molecule 1 (clone Gi18; biotinylated) Mouse Anti-Human Platelet Endothelial Cell Adhesion Molecule 1 (clone Gi18; FITC-labeled) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB RM 100 µg WB, IHC PK-AB µg WB, IHC PK-AB RM 100 µg E, FC, IHC (P), WB 100 tests 100 tests FC FC PK-AB PK-AB B PK-AB F Persephin antibody Rabbit Anti-Human Persephin 100 µg WB, E PK-AB PF-4 antibody Rabbit Anti-Human Platelet Factor µg WB, E PK-AB PKR (CT) antibody PKR (NT) antibody Rabbit Anti-Human/Mouse/Rat PKR (Interferon Inducible RNA Dependent Protein Kinase) Rabbit Anti-Human/Mouse/Rat PKR (Interferon Inducible RNA Dependent Protein Kinase) 100 µg WB PK-AB µg WB PK-AB PLGF antibody (mab) Mouse Anti-Human Placenta Growth Factor 100 µg WB, IHC PK-AB PLGF antibody (mab) Rat Anti-Mouse Placenta Growth Factor 100 µg WB, IHC PK-AB RM PLGF-1 antibody Rabbit Anti-Human Placenta Growth Factor µg WB, E PK-AB Podoplanin antibody (mab) Mouse Anti-Rat Podoplanin (Clone LF3B7D5B3) 100 µg IHC PK-AB MR Podoplanin antibody Rabbit Anti-Human Podoplanin 100 µg WB, E, IHC PK-AB PC Presenilin 1 antibody Rabbit Anti-Human/Mouse/Rat Presenilin µg WB PK-AB Presenilin 2 antibody Rabbit Anti-Human/Mouse/Rat Presenilin µg WB, IHC, IP PK-AB Prox-1 antibody Rabbit Anti-Human Prox µg WB, IF PK-AB Prox-1 antibody Rabbit Anti-Human Prox µg IHC, E PK-AB P-selectin antibody Goat Anti-Human/Mouse/Rat P-selectin 100 µg WB, IP, IHC PK-AB G PSGL-1 antibody (mab) srank Receptor antibody Mouse Anti-Human P-selectin Glycoprotein Ligand (clone PL-1) Rabbit Anti-Human soluble Receptor Activator of NF-Kappa B Receptor 100 µg E, FC, FST (BL), IHC (P), WB PK-AB µg WB, E PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

111 109 Product Name Product Description Size* Application** Catalog Number srankl antibody (mab) srankl antibody srankl antibody srankl antibody Mouse Anti-Human soluble Receptor Activator of NF-Kappa B Ligand Rabbit Anti-Mouse soluble Receptor Activator of NF-Kappa B Ligand Rabbit Anti-Human soluble Receptor Activator of NF-Kappa B Ligand Goat Anti-Human soluble Receptor Activator of NF-Kappa B Ligand 500 µg E PK-AB µg WB, E PK-AB M 100 µg WB, E PK-AB µg WB, E PK-AB G RANTES antibody (mab) Mouse Anti-Human RANTES (CCL5) 500 µg E, FST (BL) PK-AB RANTES antibody Rabbit Anti-Human RANTES (CCL5) 100 µg WB, E PK-AB RANTES antibody Rabbit Anti-Mouse RANTES (CCL5) 100 µg WB, E PK-AB M RANTES antibody Rabbit Anti-Mouse/Rat RANTES (CCL5) 100 µg WB PK-AB RANTES antibody Rabbit Anti-Rat RANTES (CCL5) 100 µg WB, E PK-AB R RELM-alpha antibody Rabbit Anti-Mouse Resistin Like Molecule alpha 100 µg WB, E PK-AB M RELM-beta antibody Rabbit Anti-Human Resistin Like Molecule beta 100 µg WB, E PK-AB RELM-beta antibody Rabbit Anti-Mouse Resistin Like Molecule beta 100 µg WB, E PK-AB M Resistin antibody (mab) Mouse Anti-Human Resistin 500 µg E PK-AB Resistin antibody Goat Anti-Human Resistin 100 µg WB, E PK-AB G Resistin antibody Goat Anti-Mouse Resistin 100 µg WB, E PK-AB GM Resistin antibody Rabbit Anti-Human Resistin 100 µg WB PK-AB Resistin antibody Rabbit Anti-Mouse Resistin 100 µg WB, IC PK-AB RP105 (CT) antibody RP105 (IN) antibody RP105 (NT) antibody Rabbit Anti-Human/Mouse Radioprotective 105 (CD180 Antigen Precursor, CT) Rabbit Anti-Human/Mouse Radioprotective 105 (CD180 Antigen Precursor, IN) Rabbit Anti-Human Radioprotective 105 (CD180 Antigen Precursor, NT) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB SCF antibody (mab) Mouse Anti-Human Stem Cell Factor 500 µg E PK-AB SCF antibody Goat Anti-Human Stem Cell Factor 100 µg WB, E, N PK-AB G SCF antibody Goat Anti-Rat Stem Cell Factor 100 µg WB, E, N PK-AB GR SCF antibody Rabbit Anti-Human Stem Cell Factor 100 µg WB, E PK-AB SCF antibody Rabbit Anti-Mouse Stem Cell Factor 100 µg WB, E PK-AB M SCF antibody Rabbit Anti-Rat Stem Cell Factor 100 µg WB PK-AB SCGF-beta antibody Goat Anti-Human Stem Cell Growth Factor beta 100 µg WB, E PK-AB G SDF-1 alpha antibody SDF-1 alpha antibody SDF-1 alpha antibody SDF-1 beta antibody Goat Anti-Mouse Stromal Cell Derived Factor 1-alpha 100 µg WB, E PK-AB GM Rabbit Anti-Human Stromal Cell Derived Factor 1-alpha Rabbit Anti-Human/Mouse/Rat Stromal Cell Derived Factor 1-alpha 100 µg WB, E PK-AB µg WB, IP, IHC PK-AB Goat Anti-Human Stromal Cell Derived Factor 1-beta 100 µg WB, E PK-AB G Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

112 110 Product Name Product Description Size* Application** Catalog Number SDF-1 beta antibody SDF-1 beta antibody Rabbit Anti-Human/Mouse/Rat Stromal Cell Derived Factor 1-beta Rabbit Anti-Mouse/Rat Stromal Cell Derived Factor 1-beta 100 µg WB PK-AB µg WB, IP, IHC PK-AB SLC antibody Rabbit Anti-Rat Secondary Lymphoid Tissue Chemokine 100 µg WB PK-AB STIM1 antibody STIM2 (CT) antibody STIM2 (IN) antibody Rabbit Anti-Human/Mouse/Rat STIM1 (Stromal Interaction Molecule 1) Rabbit Anti-Human/Mouse/Rat STIM2 (Inhibitor of STIM1, CT) Rabbit Anti-Human/Mouse/Rat STIM2 (Inhibitor of STIM1, IN) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB Synapsin antibody Rabbit Anti-Human/Mouse/Rat Synapsin 100 µg WB PK-AB TACI antibody TARC antibody Goat Anti-Human Transmembrane Activator and CAML Interactor (TNFRSF13B) Rabbit Anti-Human Thymus & Activation Regulated Chemokine 100 µg WB, E PK-AB G 100 µg WB, E PK-AB TCR alpha antibody Rabbit Anti-Human T-cell Antigen Receptor alpha 100 µg WB, IP, IHC PK-AB TCR beta antibody Rabbit Anti-Human/Mouse/Rat T-cell Antigen Receptor beta 100 µg WB, IP, IHC PK-AB TECK antibody (mab) Mouse Anti-Human Thymus Expressed Chemokine 500 µg E PK-AB TECK antibody Rabbit Anti-Human Thymus Expressed Chemokine 100 µg WB, E PK-AB TFF-1 antibody Rabbit Anti-Human Trefoil Factor Peptide µg WB PK-AB TFF-2 antibody Rabbit Anti-Human Trefoil Factor Peptide µg WB PK-AB TGF-alpha antibody Rabbit Anti-Human Transforming Growth Factor alpha 100 µg WB, E PK-AB TGF-beta antibody (mab) Mouse Anti-Human Transforming Growth Factor beta 500 µg E PK-AB TGF-beta 1 (mature) antibody TGF-beta 1 antibody TGF-beta 2 antibody TGF-beta 3 antibody TGF-beta 5 antibody TGF-beta R1 antibody TGF-beta R2 antibody TGF-beta R3 antibody TGFR-1 /ALK-1 antibody (mab) TGFR-1 /ALK-1 antibody (mab) TGFR-1 /ALK-5 antibody (mab) Rabbit Anti-Mouse/Rat/Pig Transforming Growth Factor beta-1 Rabbit Anti-Human/Mouse/Rat/Pig Transforming Growth Factor beta-2 Rabbit Anti-Human/Mouse/Rat/Pig/Dog/Sheep Transforming Growth Factor beta-2 Goat Anti-Human/Mouse/Rat Transforming Growth Factor beta-3 Goat Anti-Human/Mouse/Rat Transforming Growth Factor beta-5 Rabbit Anti-Human Transforming Growth Factor beta Receptor 1 Goat Anti-Human Transforming Growth Factor beta Receptor 2 Goat Anti-Human Transforming Growth Factor beta Receptor µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, E, N PK-AB µg WB, E, N PK-AB G 100 µg WB, E, N PK-AB G 100 µg WB, IHC PK-AB µg WB, IHC PK-AB G 100 µg WB PK-AB G Mouse Anti-Human TGF-beta Receptor Type I (ALK-1) 200 µg WB PK-AB Rat Anti-Mouse TGF-beta Receptor Type I (ALK-1) 200 µg WB, IHC PK-AB RM Mouse Anti-Human TGF-beta Receptor Type I (ALK-5) 200 µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

113 111 Product Name Product Description Size* Application** Catalog Number TGFR-1 /ALK-5 antibody (mab) Rat Anti-Mouse TGF-beta Receptor Type I (ALK-5) 200 µg WB, IHC PK-AB RM TIE-1 antibody (mab) Mouse Anti-Human TIE-1 (clone 6F12) 100 µg WB, E PK-AB TIE-1 antibody (mab) Mouse Anti-Human TIE-1 (clone 8C9) 100 µg WB, E PK-AB TIE-2 antibody (mab) Mouse Anti-Human TIE-2 (TEK, clone 2) 100 µg WB, E, FC PK-AB TIE-2 antibody (mab) Mouse Anti-Human TIE-2 (TEK, clone 9) 100 µg WB, E, FC PK-AB TIE-2 antibody (mab) Mouse Anti-Human TIE-2 (TEK, clone 16) 100 µg WB, E, FC PK-AB TIM-1 / Kim-1 (IN) antibody TIM-1 / Kim-1 (NT) antibody TIM-4 / SMUCKLER (CT) antibody TIM-4 / SMUCKLER (IN) antibody TIMP-1 antibody (mab) TIMP-2 antibody (mab) TIMP-3 antibody (mab) TIMP-4 antibody (mab) Rabbit Anti-Human/Mouse/Rat T-cell Immunoglobulin & Mucin Domain Containing Protein 1 (Kim-1, IN) Rabbit Anti-Human/Mouse T-cell Immunoglobulin & Mucin Domain Containing Protein 1 (Kim-1, NT) Rabbit Anti-Human/Mouse/Rat T-cell Immunoglobulin & Mucin Domain Containing Protein 4 (SMUCKLER, CT) Rabbit Anti-Human/Mouse T-cell Immunoglobulin & Mucin Domain Containing Protein 4 (SMUCKLER, IN) Mouse Anti-Human Tissue Inhibitor of Matrix Metalloprotease 1 Mouse Anti-Human Tissue Inhibitor of Matrix Metalloprotease 2 Mouse Anti-Human Tissue Inhibitor of Matrix Metalloprotease 3 Mouse Anti-Human Tissue Inhibitor of Matrix Metalloprotease µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC (P) PK-AB µg WB PK-AB µg WB, IHC (P) PK-AB µg WB, IHC (P) PK-AB TL-1 antibody Rabbit Anti-Human TNF-Like µg WB, E PK-AB TNF-alpha antibody (mab) Mouse Anti-Human Tumor Necrosis Factor alpha 500 µg WB, E, IP, IS PK-AB TNF-alpha antibody (mab) TNF-alpha antibody (mab) TNF-alpha antibody (mab) Mouse Anti-Human Tumor Necrosis Factor alpha (clone MP6-XT22) Mouse Anti-Human Tumor Necrosis Factor alpha (clone TNF-D) Rat Anti-Mouse Tumor Necrosis Factor alpha (clone 1F3F3D4 IGH 156) 50 µg WB, IP, IHC PK-AB µg E, FC, FST (BL) PK-AB µg E, FST (BL) PK-AB RM TNF-alpha antibody Goat Anti-Human Tumor Necrosis Factor alpha 100 µg WB, E PK-AB G TNF-alpha antibody Goat Anti-Mouse Tumor Necrosis Factor alpha 100 µg WB, E PK-AB GM TNF-alpha antibody Rabbit Anti-Human Tumor Necrosis Factor alpha 100 µg WB, E PK-AB TNF-alpha antibody Rabbit Anti-Mouse Tumor Necrosis Factor alpha 100 µg WB, E PK-AB M TNF-alpha antibody Rabbit Anti-Rat Tumor Necrosis Factor alpha 100 µg WB, E PK-AB R TNF-beta antibody (mab) TNF-beta antibody (mab), FITC Mouse Anti-Human Tumor Necrosis Factor beta (clone LTX-21) Mouse Anti-Human Tumor Necrosis Factor beta (clone LTX-21; FITC-labeled) 100 µg FST (BL) PK-AB tests not tested PK-AB F TNF-beta antibody Rabbit Anti-Human Tumor Necrosis Factor beta 100 µg WB, E PK-AB stnf-r (60 kda) antibody (mab) Mouse Anti-Human Tumor Necrosis Factor Receptor (60 kda; clone H398) 100 µg FC, FST (BL), IHC PK-AB stnf-ri antibody (mab) Hamster Anti-Mouse TNF Receptor I 100 µg WB, IP, FC PK-AB HM stnf-rii antibody (mab) Hamster Anti-Mouse TNF Receptor II 100 µg WB, IP, FC PK-AB HM Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

114 112 Product Name Product Description Size* Application** Catalog Number stnf-ri antibody stnf-rii antibody TNFRSF14 / HVEM / TR2 (CT) antibody TNFRSF14 / HVEM / TR2 (NT) antibody Rabbit Anti-Human soluble Tumor Necrosis Factor Receptor I Rabbit Anti-Human soluble Tumor Necrosis Factor Receptor II Rabbit Anti-Human Tumor Necrosis Factor Receptor (Superfamily 14) Rabbit Anti-Human/Mouse Tumor Necrosis Factor Receptor (Superfamily 14) 100 µg WB, E PK-AB µg WB, E PK-AB µg WB PK-AB µg WB PK-AB TPO antibody Rabbit Anti-Human Thrombopoietin 100 µg WB PK-AB TPO antibody Goat Anti-Human Thrombopoietin 100 µg WB, E PK-AB G TRAIL / Apo2L antibody (mab) TRAIL / Apo2L antibody Mouse Anti-Human TNF-Related Apoptosis Inducing Ligand Rabbit Anti-Human Tumor Necrosis Factor Related Apoptosis Inducing Ligand 500 µg E PK-AB µg WB, E PK-AB TSLP antibody Rabbit Anti-Human Thymic Stromal Lymphopoietin 100 µg WB, E PK-AB TSLP antibody Rabbit Anti-Mouse/Rat Thymic Stromal Lymphopoietin 100 µg WB, IHC PK-AB TSLP (CT) antibody Rabbit Anti-Human Thymic Stromal Lymphopoietin (CT) 100 µg WB, IHC PK-AB TSLP (IN) antibody TSLP Receptor antibody TSLP Receptor antibody Rabbit Anti-Human/Mouse Thymic Stromal Lymphopoietin (IN) Rabbit Anti-Human Thymic Stromal Lymphopoietin Receptor Rabbit Anti-Mouse Thymic Stromal Lymphopoietin Receptor 100 µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB TWEAK antibody Goat Anti-Human TNF Related Weak Inducer of Apoptosis 100 µg WB, E PK-AB G Vaspin antibody Rabbit Anti-Human Vaspin 100 µg WB, E PK-AB VCAM-1 / CD106 antibody (mab) VCAM-1 / CD106 antibody (mab) VCAM-1 / CD106 antibody (mab), biotin VE-Cadherin antibody (mab) Rat Anti-Mouse Vascular Cell Adhesion Molecule 1 (CD106) Mouse Anti-Human Vascular Cell Adhesion Molecule 1 (CD106; clone B-N8) Mouse Anti-Human Vascular Cell Adhesion Molecule 1 (CD106; clone B-N8; biotinylated) 100 µg WB, IHC PK-AB RM 100 µg E, FC, FST, IHC 100 tests FC PK-AB PK-AB B Mouse Anti-Human VE-Cadherin 100 µg WB PK-AB VEGF-A antibody (mab) Mouse Anti-Human Vascular Endothelial Growth Factor 500 µg WB, E, IP PK-AB VEGF-A antibody (mab) VEGF-A antibody (mab) VEGF-A antibody (mab) VEGF-A antibody (mab) Mouse Anti-Human Vascular Endothelial Growth Factor A (clone 1) Mouse Anti-Human Vascular Endothelial Growth Factor A (clone 3) Mouse Anti-Human Vascular Endothelial Growth Factor A (clone 3C5) Mouse Anti-Human Vascular Endothelial Growth Factor A (clone 7G7) 100 µg WB, IHC PK-AB µg E, WB, FST (BL) PK-AB µg E, WB PK-AB µg E, WB PK-AB VEGF-A antibody (mab) Rat Anti-Mouse Vascular Endothelial Growth Factor A 100 µg WB, IHC PK-AB RM VEGF-A antibody Goat Anti-Human Vascular Endothelial Growth Factor A 100 µg WB, E PK-AB G VEGF-A antibody Rabbit Anti-Human Vascular Endothelial Growth Factor A 100 µg WB, E PK-AB VEGF-A antibody Rabbit Anti-Mouse Vascular Endothelial Growth Factor A 100 µg WB, E PK-AB M Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

115 113 Product Name Product Description Size* Application** Catalog Number VEGF-A antibody Rabbit Anti-Rat Vascular Endothelial Growth Factor A 100 µg WB, E, N PK-AB RPC VEGF-B antibody (mab) Mouse Anti-Human Vascular Endothelial Growth Factor B 100 µg WB, IHC PK-AB VEGF-B antibody Rat Anti-Mouse Vascular Endothelial Growth Factor B 100 µg WB, IHC PK-AB MPC VEGF-C antibody (mab) Mouse Anti-Human Vascular Endothelial Growth Factor C (flt-4 Ligand) 100 µg WB PK-AB VEGF-C antibody Rabbit Anti-Rat Vascular Endothelial Growth Factor C 100 µg WB, E, IP PK-AB PC VEGF-C antibody Goat Anti-Human Vascular Endothelial Growth Factor C 100 µg WB, IHC PK-AB G VEGF-D antibody (mab) VEGF-D antibody Mouse Anti-Human Vascular Endothelial Growth Factor D Rabbit Anti-Human/Mouse/Rat Vascular Endothelial Growth Factor D 100 µg WB, IHC PK-AB µg WB, E PK-AB VEGF-D antibody Rat Anti-Mouse Vascular Endothelial Growth Factor D 100 µg WB, IHC PK-AB MPC VEGFR-1 / flt-1 antibody (mab) VEGFR-1 / flt-1 antibody (mab) VEGFR-1 / flt-1 antibody (mab) VEGFR-1 / flt-1 antibody (mab) VEGFR-1 / flt-1 antibody (mab) VEGFR-1 / flt-1 antibody (mab) VEGFR-1 / flt-1 antibody VEGFR-2 / FLK-1 antibody (mab) VEGFR-2 / KDR antibody (mab) VEGFR-2 / KDR antibody (mab) VEGFR-2 / KDR antibody (mab) VEGFR-2 / KDR antibody (mab) VEGFR-2 / KDR antibody (mab) VEGFR-2 / KDR antibody VEGFR-3 / flt-4 antibody (mab) VEGFR-3 / flt-4 antibody (mab) VEGFR-3 / flt-4 antibody (mab) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 1 (flt-1) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 1 (flt-1; clone EIC) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 1 (flt-1; clone EWC) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 1 (flt-1; clone EWF) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 1 (flt-1; clone EWI) Rat Anti-Mouse Vascular Endothelial Growth Factor Receptor 1 (flt-1; clone EWI; NT) Rabbit Anti-Human Vascular Endothelial Growth Factor Receptor 1 (flt-1) Rat Anti-Mouse Vascular Endothelial Growth Factor Receptor 2 (FLK; clone 5) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 2 (KDR; clone 3) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 2 (KDR; clone 4) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 2 (KDR; clone 5) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 2 (KDR; clone EIC) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 2 (KDR; clone EWC) Rabbit Anti-Human Vascular Endothelial Growth Factor Receptor 2 (KDR) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 3 (flt-4; clone 1) Mouse Anti-Human Vascular Endothelial Growth Factor Receptor 3 (flt-4; clone 3) Rat Anti-Mouse Vascular Endothelial Growth Factor Receptor 3 (flt-4; clone 3) 100 µg E, IHC, IP PK-AB µg E, IHC, FC PK-AB µg E, WB, IP PK-AB µg E, WB, IP PK-AB µg E, WB, IP PK-AB µg WB, IHC PK-AB RM 100 µg WB, E, IHC PK-AB PC 100 µg WB, IHC PK-AB RM 100 µg E, WB, FC PK-AB µg E, WB, IHC, FC, IF PK-AB µg WB, IHC PK-AB µg E, IHC, FC PK-AB µg E, WB, FC PK-AB µg WB, E PK-AB PC 100 µg E, WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB RM Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

116 114 Product Name Product Description Size* Application** Catalog Number VEGFR-3 / flt-4 antibody Rabbit Anti-Human Vascular Endothelial Growth Factor Receptor 3 (flt-4) 100 µg WB, E PK-AB PC Vimentin antibody Rabbit Anti-Human/Mouse/Rat/Bovine/Chicken Vimentin 100 µg WB, IP, IHC PK-AB Visfatin antibody Rabbit Anti-Human/Mouse Visfatin 100 µg WB PK-AB Visfatin antibody Rabbit Anti-Mouse Visfatin 100 µg WB PK-AB Visfatin antibody Rabbit Anti-Rat Visfatin 100 µg WB PK-AB Wnt-1 antibody Rabbit Anti-Human Wnt µg WB, E PK-AB Wnt-2 antibody Rabbit Anti-Human/Mouse/Rat Wnt µg WB PK-AB R Wnt-3a antibody Rabbit Anti-Human Wnt-3a 100 µg WB, E PK-AB Wnt-4 antibody Rabbit Anti-Human/Mouse/Rat Wnt µg WB PK-AB R Wnt-10a antibody Rabbit Anti-Human/Mouse/Rat Wnt-10a 100 µg WB PK-AB Wnt-10b antibody Rabbit Anti-Human/Mouse/Rat Wnt-10b (Wnt-12) 100 µg WB PK-AB n Signal Transduction Product Name Product Description Size* Application** Catalog Number 4E-BP1 antibody Rabbit Anti-Human/Mouse 4E-BP1 (Eukaryotic Translation Initiation Factor) 100 µg WB PK-AB Adiponectin / Acrp30 antibody (mab) Mouse Anti-Human Adiponectin (Adipocyte Complement Related Protein of 30 kda; clone P1G12AT) 50 µg E, WB PK-AB Adiponectin / Acrp30 antibody (mab) Mouse Anti-Human/Mouse/Rat Adiponectin (Adipocyte Complement Related Protein of 30 kda; clone BV801) 50 µg WB, E PK-AB Adiponectin / Acrp30 (CT) antibody Rabbit Anti-Human/Mouse Adipokine (Adipocyte Complement Related Protein of 30 kda, CT) 100 µg WB, IHC PK-AB Adiponectin / Acrp30 (NT) antibody Rabbit Anti-Human/Mouse/Rat Adipokine (Adipocyte Complement Related Protein of 30 kda, NT) 100 µg WB, IHC PK-AB Akt1 / PKB antibody Rabbit Anti-Human/Mouse/Rat Akt1 (PKB, Serine/Threonine Kinase) 100 µg WB PK-AB ATF6 (CT) antibody ATF6 (NT) antibody BAP29 (CT) antibody Rabbit Anti-Human/Mouse Activating Transcription Factor 6 (CT) Rabbit Anti-Human/Mouse Activating Transcription Factor 6 (NT) Rabbit Anti-Human/Mouse/Rat B-cell Receptor Associated Protein 29 (CT) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB BAP29 (IN) antibody Rabbit Anti-Human/Mouse/Rat B-cell Receptor Associated Protein 29 (IN) 100 µg WB PK-AB BAP31 (CT) antibody Rabbit Anti-Human/Mouse/Rat B-cell Receptor Associated Protein 31 (CT) 100 µg WB PK-AB BAP31 (IN) antibody Rabbit Anti-Human/Mouse/Rat B-cell Receptor Associated Protein 31 (IN) 100 µg WB PK-AB Cbl antibody Rabbit Anti-Human/Mouse/Rat Casitas B-lineage Lymphoma Proto-Oncogene 100 µg WB PK-AB CDKN2A antibody Rabbit Anti-Human/Mouse/Rat Cyclin-dependent Kinase Inhibitor 2A 100 µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

117 115 Product Name Product Description Size* Application** Catalog Number CIKS / Act1 (CT) antibody CIKS / Act1 (NT) antibody Rabbit Anti-Human CIKS (Act1, NF-kB Activator, CT) 100 µg WB PK-AB Rabbit Anti-Human CIKS (Act1, NF-kB Activator, NT) 100 µg WB PK-AB c-jun antibody Rabbit Anti-Human/Mouse/Rat c-jun 100 µg WB, IHC PK-AB CTRP1 / GIP (IN) antibody CTRP1 / GIP (NT) antibody CTRP2 (IN) antibody CTRP2 (NT) antibody CTRP2 antibody CTRP3 / CORS26 (IN) antibody CTRP4 (CT) antibody CTRP4 (NT) antibody CTRP5 (IN) antibody CTRP5 (NT) antibody CTRP6 (CT) antibody CTRP6 (IN) antibody CTRP7 (CT) antibody CTRP7 (NT) antibody CTRP7 antibody DARC (CT) antibody DARC (NT) antibody DC Marker antibody (mab) GAPDH / G3PDH (NT) antibody GAPDH / G3PDH (CT) antibody GITRL / AITRL antibody Rabbit Anti-Human/Mouse C1q/Tumor Necrosis Factor-alpha Related Protein 1 (Novel Adipokine, IN) Rabbit Anti-Human/Mouse/Rat C1q/Tumor Necrosis Factor-alpha Related Protein 1 (Novel Adipokine, NT) Rabbit Anti-Human/Mouse C1q/Tumor Necrosis Factor-alpha Related Protein 2 (Novel Adipokine, IN) Rabbit Anti-Human/Mouse C1q/Tumor Necrosis Factor-alpha Related Protein 2 (Novel Adipokine, NT) Rabbit Anti-Human/Mouse/Rat C1q/Tumor Necrosis Factor-alpha Related Protein 2 (Novel Adipokine, NT) Rabbit Anti-Human/Mouse C1q/Tumor Necrosis Factor-alpha Related Protein 3 (Novel Adipokine, IN) Rabbit Anti-Human/Mouse/Rat C1q/Tumor Necrosis Factor-alpha Related Protein 4 (Novel Adipokine, CT) Rabbit Anti-Human/Mouse/Rat C1q/Tumor Necrosis Factor-alpha Related Protein 4 (Novel Adipokine, NT) Rabbit Anti-Human C1q/Tumor Necrosis Factor-alpha Related Protein 5 (Novel Adipokine, IN) Rabbit Anti-Human/Rat C1q/Tumor Necrosis Factoralpha Related Protein 5 (Novel Adipokine, NT) Rabbit Anti-Human/Mouse C1q/Tumor Necrosis Factor-alpha Related Protein 6 (Novel Adipokine, CT) Rabbit Anti-Human/Mouse/Rat C1q/Tumor Necrosis Factor-alpha Related Protein 6 (Novel Adipokine, IN) Rabbit Anti-Human/Mouse/Rat C1q/Tumor Necrosis Factor-alpha Related Protein 7 (Novel Adipokine, CT) Rabbit Anti-Human/Mouse/Rat C1q/Tumor Necrosis Factor-alpha Related Protein 7 (Novel Adipokine, NT) Rabbit Anti-Human/Mouse/Rat C1q/Tumor Necrosis Factor-alpha Related Protein 7 (Novel Adipokine) Rabbit Anti-Human/Mouse/Rat DARC (Duffy Antigen/Chemokine Receptor, CT) Rabbit Anti-Human DARC (Duffy Antigen/Chemokine Receptor, NT) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB Rat Anti-Mouse Dendritic Cell Marker 200 µg IHC (P), FC PK-AB RM Rabbit Anti-Human/Mouse/Rat GAPDH / G3PDH / GAPD (Enzyme in the Glycolytic Pathway, NT) Rabbit Anti-Human/Mouse/Rat GAPDH / G3PDH / GAPD (Enzyme in the Glycolytic Pathway, CT) Rabbit Anti-Human/Mouse Glucocorticoid Induced TNF Receptor Ligand (AITRL) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB GSK-3α antibody Rabbit Anti-Human/Mouse/Rat GSK-3 alpha 100 µg WB, IP, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

118 116 Product Name Product Description Size* Application** Catalog Number GSK-3b antibody Rabbit Anti-Human/Mouse/Rat GSK-3 beta 100 µg WB, IP, IHC PK-AB GbL antibody Hrk antibody IKAP antibody IKKα / IKK-1 (C1) antibody IKKα / IKK-1 (C2) antibody IKKα / IKK-1 (C3) antibody IKKβ / IKK-2 (C3) antibody IKKγ / NEMO / FIP3 (NT) antibody IKKε / IKKi (CT) antibody IL-1RAcP (C1) antibody IL-1RAcP (C2) antibody IRAK (CT) antibody IRAK2 (C2) antibody IRAK-2 (C2) antibody IRAK2 (CT) antibody IRAK-2 (CT) antibody Rabbit Anti-Human/Mouse/Rat GbL (Member of the TOR Signaling Pathway) Rabbit Anti-Human/Mouse Harakiri (Neuronal Death Protein DP5) Rabbit Anti-Human/Mouse IKK Complex Associated Protein (IKBKAP) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB Rabbit Anti-Human IkB Kinase alpha (IKK-1, C1) 100 µg WB PK-AB Rabbit Anti-Human IkB Kinase alpha (IKK-1, C2) 100 µg WB PK-AB Rabbit Anti-Human IkB Kinase alpha (IKK-1, C3) 100 µg WB PK-AB Rabbit Anti-Human IkB Kinase beta (IKK-1, C3) 100 µg WB, IHC PK-AB Rabbit Anti-Human/Mouse/Rat IkB Kinase gamma (NEMO, FIP3, NT) 100 µg WB PK-AB Rabbit Anti-Human IkB Kinase epsilon (IKKi, CT) 100 µg WB, IHC PK-AB Rabbit Anti-Human IL-1R Accessory Protein (C1) 100 µg WB PK-AB Rabbit Anti-Human IL-1R Accessory Protein (C2) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat IL-1R Associated Kinase (CT) 100 µg WB, IP PK-AB Rabbit Anti-Mouse IL-1R Associated Kinase 2 (C2) 100 µg WB PK-AB Rabbit Anti-Human IL-1R Associated Kinase 2 (C2) 100 µg WB, IHC PK-AB Rabbit Anti-Human/Mouse IL-1R Associated Kinase 2 (CT) 100 µg WB PK-AB Rabbit Anti-Human IL-1R Associated Kinase 2 (CT) 100 µg WB PK-AB IRAK-4 antibody Rabbit Anti-Human IL-1R Associated Kinase µg WB PK-AB IRAK-M antibody IRE1p / ERN1 (CT) antibody IRE1p / ERN1 (IN) antibody IRE1p / ERN1 (NT) antibody IRS-1 antibody Rabbit Anti-Human/Mouse/Rat IL-1R Associated Kinase M Rabbit Anti-Human/Mouse/Rat IRE1p (ERN1, Endoplasmic Reticulum-to-Nucleus Signaling 1, CT) Rabbit Anti-Human IRE1p (ERN1, Endoplasmic Reticulum-to-Nucleus Signaling 1, IN) Rabbit Anti-Human/Mouse/Rat IRE1p (ERN1, Endoplasmic Reticulum-to-Nucleus Signaling 1, NT) Rabbit Anti-Human/Mouse IRS-1 (Scaffolding Protein Important for Insulin Receptor Signaling) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB JNK1 antibody Rabbit Anti-Human/Mouse/Rat JNK1 100 µg WB, IP, IHC PK-AB KAI1 (CT) antibody Rabbit Anti-Human/Mouse/Rat KAI1 (Tumor Metastasis Suppressor Gene, CT) 100 µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

119 117 Product Name Product Description Size* Application** Catalog Number KappaB ras antibody KappaB ras1 antibody KappaB ras2 antibody LKB1 antibody MADD / DENN (CT) antibody MAPKAP1 (IN) antibody MAPKAP1 (NT) antibody MyD88 (CT) antibody MyD88 (IN) antibody NAK / TBK1 (CT) antibody Nanos1 (IN) antibody Nanos1 (NT) antibody Nanos2 (CT) antibody Nanos2 (NT) antibody Nanos3 (CT) antibody NGFR / TNFRSF16 antibody Nudel antibody p62dok (CT) antibody PAK2 (CT) antibody PAK2 (NT) antibody Rabbit Anti-Human/Mouse/Rat KappaB ras (NF-kappaB Inhibitor-interacting Ras-like Protein) Rabbit Anti-Human/Mouse KappaB ras1 (NF-kappaB Inhibitor-interacting Ras-like Protein) Rabbit Anti-Human/Mouse/Rat KappaB ras2 (NF-kappaB Inhibitor-interacting Ras-like Protein) Rabbit Anti-Human/Mouse LKB1 (Serine-Threonine Protein Kinase 11) Rabbit Anti-Human/Mouse/Rat MAP Kinase Activating Death Domain Protein (Activator of MAPK Pathway, CT) Rabbit Anti-Human/Mouse/Rat Mitogen-activated Protein Kinase Associated Protein 1 (IN) Rabbit Anti-Human/Mouse/Rat Mitogen-activated Protein Kinase Associated Protein 1 (NT) Rabbit Anti-Human/Mouse/Rat MyD88 (Adaptor in IL-1 & LPS Signaling, CT) Rabbit Anti-Human MyD88 (Adaptor in IL-1 & LPS Signaling, IN) Rabbit Anti-Human/Mouse NF-kB Activating Kinase (TBK1, CT) 100 µg WB, IHC PK-AB µg WB, ICC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Nanos 1 (NOS1, IN) 100 µg WB PK-AB Rabbit Anti-Human Nanos 1 (NT) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Nanos 2 (CT) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Nanos 2 (NT) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Nanos 3 (CT) 100 µg WB PK-AB Rabbit Anti-Human/Mouse Nerve Growth Factor Receptor (TNFRSF16) Rabbit Anti-Human/Mouse Nudel (Protein Involved in Neuronal Development) Rabbit Anti-Human p62dok (Tyrosine Kinase Substrate p62 DOK, CT) Rabbit Anti-Human/Mouse/Rat p21-activated Kinase 2 (PAK65, CT) Rabbit Anti-Human/Mouse/Rat p21-activated Kinase 2 (PAK65, NT) 100 µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB PAK4 antibody Rabbit Anti-Human p21-activated Kinase µg WB PK-AB PAK5 antibody Rabbit Anti-Human p21-activated Kinase µg WB PK-AB PAK6 antibody Rabbit Anti-Human p21-activated Kinase µg WB PK-AB Precerebellin / Cbln1 (CT) antibody Precerebellin / Cbln1 (IN) antibody Rabbit Anti-Human/Mouse/Rat Precerebellin (Neuromodulatory Protein Precursor, CT) Rabbit Anti-Human/Mouse/Rat Precerebellin (Neuromodulatory Protein Precursor, IN) 100 µg WB PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

120 118 Product Name Product Description Size* Application** Catalog Number PTEN / MMAC / TEP-1 (CT) antibody PTEN / MMAC / TEP-1 (NT) antibody Raptor (CT) antibody Raptor (IN) antibody Raptor (NT) antibody Rheb (IN) antibody Rheb (NT) antibody RPS6K1 / p70-s6k antibody SATB1 (CT) antibody Rabbit Anti-Human/Mouse PTEN / MMAC / TEP-1 (Phosphatase and Tensin Homolog, CT) Rabbit Anti-Human/Mouse PTEN / MMAC / TEP-1 (Phosphatase and Tensin Homolog, NT) Rabbit Anti-Mouse Raptor (Member of the TOR Signaling Pathway, CT) Rabbit Anti-Human/Mouse Raptor (Member of the TOR Signaling Pathway, IN) Rabbit Anti-Human/Mouse Raptor (Member of the TOR Signaling Pathway, NT) Rabbit Anti-Human/Mouse/Rat Rheb (Ras Homolog Enriched in Brain, IN) Rabbit Anti-Human/Mouse/Rat Rheb (Ras Homolog Enriched in Brain, NT) Rabbit Anti-Human RPS6K1 (p70-s6k, Kinase Involved in Cell Growth & Metabolism) Rabbit Anti-Human/Mouse/Rat SATB1 (Special AT-rich sequence-binding Protein 1, CT) 100 µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB SATB1 (NT) antibody SATB2 (CT) antibody SATB2 (NT) antibody SHP2 / PTP-2C (CT) antibody SHP2 / PTP-2C (NT) antibody SIRPa / SHPS-1 (CT) antibody Ski antibody Rabbit Anti-Human/Mouse/Rat SATB1 (Special AT-rich sequence-binding Protein 1, NT) Rabbit Anti-Human/Mouse/Rat SATB2 (DNA-binding Protein SATB2, CT) Rabbit Anti-Human/Mouse/Rat SATB2 (DNA-binding Protein SATB2, NT) Rabbit Anti-Human/Mouse/Rat SHP2 (PTP-2C, Tyrosine Protein Phosphatase Non-Receptor Type 11, CT) Rabbit Anti-Human/Mouse/Rat SHP2 (PTP-2C, Tyrosine Protein Phosphatase Non-Receptor Type 11, NT) Rabbit Anti-Human/Mouse/Rat SIRPa (SHPS-1, Tyrosine Kinase Substrate, CT) Rabbit Anti-Human Ski (Homolog of a Retroviral Oncogene) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IF PK-AB SkiP antibody Rabbit Anti-Human/Mouse/Rat Ski-interacting Protein 100 µg WB, IF PK-AB Slug (IN1) antibody Slug (IN2) antibody Rabbit Anti-Human/Mouse Neural Crest Transcription Factor Slug (IN1) Rabbit Anti-Human/Mouse Neural Crest Transcription Factor Slug (IN2) 100 µg WB PK-AB µg WB PK-AB SnoN antibody Rabbit Anti-Human/Mouse/Rat SnoN (Ski-like Protein) 100 µg WB, IHC PK-AB SOCS1 / JAB (NT) antibody SOCS1 / JAB (CT) antibody STAT1 (CT) antibody Sumo antibody TAK1 / MAP3K7 (NT) antibody Rabbit Anti-Human Suppressor of Cytokine Signaling 1 (JAB, NT) Rabbit Anti-Human/Mouse/Rat Suppressor of Cytokine Signaling 1 (JAB, CT) Rabbit Anti-Human/Mouse/Rat STAT1 (JAK Activated Transcription, CT) Rabbit Anti-Human/Mouse/Rat Sumo (Small Ubiquitin-Like Modifier 1) Rabbit Anti-Human/Mouse/Rat TGF-beta Activated Protein Kinase 1 (MAP3K7, NT) 100 µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

121 119 Product Name Product Description Size* Application** Catalog Number TANK / TBK1 (CT) antibody TANK / TBK1 (NT) antibody T-cadherin / Heart-cadherin (NT) antibody Rabbit Anti-Human/Mouse/Rat TRAF Family Member Associated NF-kappaB Activator (TBK1, CT) Rabbit Anti-Human TRAF Family Member Associated NF-kappaB Activator (TBK1, NT) Rabbit Anti-Human/Mouse T-cadherin (Heart-cadherin, Cell Adhesion Molecule, NT) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB TIP47 (CT) antibody Rabbit Anti-Human/Rat Tail Interacting Protein 47 (CT) 100 µg WB PK-AB TIP47 (NT) antibody TOR / FRAP / RAFT antibody TRAF2 (CT) antibody TRAF2 (NT) antibody TRAF3 / CD40BP / LAP1 (CT) antibody TRAF3 / CD40BP / LAP1 (NT) antibody TRAF6 (CT) antibody TRPC3 (CT) antibody TRPC3 (NT) antibody TRPC6 (CT) antibody TRPC6 (NT) antibody TSC1 / Hamartin (CT) antibody TSC1 / Hamartin (IN) antibody TSC2 / Tuberin (CT) antibody TSC2 / Tuberin (NT) antibody TTC5 antibody Rabbit Anti-Human/Mouse/Rat Tail Interacting Protein 47 (NT) Rabbit Anti-Human/Mouse TOR (FRAP, RAFT, Target of Rapamycin) Rabbit Anti-Human/Mouse/Rat TNF Receptor Associated Factor 2 (CT) Rabbit Anti-Human/Mouse/Rat TNF Receptor Associated Factor 2 (NT) Rabbit Anti-Human/Mouse TNF Receptor Associated Factor 3 (CD40BP, LAP1, CT) Rabbit Anti-Human/Mouse/Rat TNF Receptor Associated Factor 3 (CD40BP, LAP1, NT) Rabbit Anti-Human/Mouse/Rat TNF Receptor Associated Factor 6 (CT) Rabbit Anti-Human/Mouse/Rat Transient Receptor Potential Channel 3 (CT) Rabbit Anti-Human/Mouse Transient Receptor Potential Channel 3 (NT) Rabbit Anti-Human/Mouse/Rat Transient Receptor Potential Channel 6 (CT) Rabbit Anti-Human/Mouse Transient Receptor Potential Channel 6 (NT) Rabbit Anti-Human/Mouse Tuberous Sclerosis Complex 1 (Member of the TOR Signaling Pathway, CT) Rabbit Anti-Human/Mouse/Rat Tuberous Sclerosis Complex 1 (Member of the TOR Signaling Pathway, IN) Rabbit Anti-Human/Mouse Tuberous Sclerosis Complex 2 (Member of the TOR Signaling Pathway, CT) Rabbit Anti-Human/Mouse Tuberous Sclerosis Complex 2 (Member of the TOR Signaling Pathway, NT) Rabbit Anti-Human/Mouse Tetratricopeptide Repeat Containing Protein µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Wnt-10a antibody Rabbit Anti-Human/Mouse/Rat Wnt-10a 100 µg WB PK-AB Wnt-10b antibody Rabbit Anti-Human/Mouse/Rat Wnt-10b (Wnt-12) 100 µg WB PK-AB XBP-1 (CT) antibody XBP-1 (NT) antibody XEDAR / TNFRSF27 antibody Rabbit Anti-Human/Mouse/Rat X-box Binding Protein 1 (CT) 100 µg WB PK-AB Rabbit Anti-Human X-box Binding Protein 1 (NT) 100 µg WB PK-AB Rabbit Anti-Human X-linked Ectodysplasin-A2 Receptor (TNFRSF27) 100 µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

122 120 n Apoptosis Product Name Product Description Size* Application** Catalog Number AATF / Che-1 antibody Acetyl-Histone H2A antibody Acetyl-Histone H2B antibody Acetyl-Histone H3 antibody Acetyl-Histone H4 antibody Acinus (CP) antibody Acinus (CT) antibody Acinus (IN) antibody Acinus (NP) antibody ADAM10 / KUZ (CT) antibody AES / GRG / ESP1 (CT) antibody AES / GRG / ESP1 (NT) antibody AIF (CT) antibody AIF (IN) antibody Rabbit Anti-Human/Mouse Anti-Apoptotic Transcription Factor (Che-1) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Acetyl-Histone H2A 100 µg WB, IP, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Acetyl-Histone H2B 100 µg WB, IP, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Acetyl-Histone H3 100 µg WB, IP, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Acetyl-Histone H4 100 µg WB, IP, IHC PK-AB Rabbit Anti-Human Acinus (Inducer of Chromatin Condensation, CP) Rabbit Anti-Human Acinus (Inducer of Chromatin Condensation, CT) Rabbit Anti-Human Acinus (Inducer of Chromatin Condensation, IN) Rabbit Anti-Human Acinus (Inducer of Chromatin Condensation, NP) Rabbit Anti-Human ADAM10 (KUZ, TNF-alpha Converting Enzyme, CT) Rabbit Anti-Human/Mouse/Rat Amino Terminal Enhancer of Split (GRG, ESP1, CT) Rabbit Anti-Human/Mouse Amino Terminal Enhancer of Split (GRG, ESP1, NT) Rabbit Anti-Human/Mouse/Rat Apoptosis Inducing Factor (CT) Rabbit Anti-Human/Mouse/Rat Apoptosis Inducing Factor (IN) 100 µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB AIF (NT) antibody Rabbit Anti-Human Apoptosis Inducing Factor (NT) 100 µg WB, IHC PK-AB AIFM3 (IN) antibody AIFM3 (NT) antibody AIM / API6 (CT) antibody AIM / API6 (NT) antibody Rabbit Anti-Human/Mouse/Rat Apoptosis Inducing Factor Mitochondrion-associated 3 (IN) Rabbit Anti-Human/Mouse/Rat Apoptosis Inducing Factor Mitochondrion-associated 3 (NT) Rabbit Anti-Human/Mouse Apoptosis Inhibitor of Macrophages (CD5L, CT) Rabbit Anti-Human/Mouse Apoptosis Inhibitor of Macrophages (CD5L, NT) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Annexin V antibody (mab) Mouse Anti-Human Annexin V (clone VAA-33) 100 µg E, FC PK-AB Annexin V antibody (mab), biotin Annexin V antibody (mab), FITC Mouse Anti-Human Annexin V (clone VAA-33; biotinylated) Mouse Anti-Human Annexin V (clone VAA-33; FITC-labeled) 100 tests 100 tests FC FC PK-AB B PK-AB F Annexin V antibody Rabbit Anti-Human/Mouse/Rat Annexin V 100 µg WB, IP, IHC PK-AB Apaf-1 (CT) antibody Apaf-1 (NT) antibody Rabbit Anti-Human/Mouse/Rat Apoptosis Protease Activating Factor 1 (CT) Rabbit Anti-Human/Mouse/Rat Apoptosis Protease Activating Factor 1 (NT) 100 µg WB, IHC PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

123 121 Product Name Product Description Size* Application** Catalog Number APG7 / ATG7 (CT) antibody APG7 / ATG7 (NT) antibody APO-1/Fas (2R2) antibody (mab) APO-1/Fas (APO-1-1) antibody (mab) APO-1/Fas (APO-1-1) antibody (mab), biotin APO-1/Fas (APO-1-1) antibody (mab), FITC APO-1/Fas (SM1/1) antibody (mab) APO-1/Fas (SM1/23) antibody (mab) APO-1/Fas (SM1/23) antibody (mab), biotin APO-1/Fas (SM1/23) antibody (mab), FITC ARC (CT) antibody ARC (NT) antibody Rabbit Anti-Human/Mouse APG7 (ATG7, Autophagy Related Protein, CT) Rabbit Anti-Human/Mouse APG7 (ATG7, Autophagy Related Protein, NT) Mouse Anti-Human Apoptosis Antigen 1/Fas (clone 2R2) Mouse Anti-Human Apoptosis Antigen 1/Fas (clone APO-1-1) Mouse Anti-Human Apoptosis Antigen 1/Fas (clone APO-1-1; biotinylated) Mouse Anti-Human Apoptosis Antigen 1/Fas (clone APO-1-1; FITC-labeled) Mouse Anti-Human Apoptosis Antigen 1/Fas (clone SM1/1) Mouse Anti-Human Apoptosis Antigen 1/Fas (clone SM1/23) Mouse Anti-Human Apoptosis Antigen 1/Fas (clone SM1/23; biotinylated) Mouse Anti-Human Apoptosis Antigen 1/Fas (clone SM1/23; FITC-labeled) Rabbit Anti-Human Apoptosis Repressor with CARD Domain (CT) Rabbit Anti-Human/Mouse/Rat Apoptosis Repressor with CARD Domain (NT) 100 µg WB PK-AB µg WB PK-AB µg FC, FST (AI) PK-AB µg FC, IHC PK-AB tests 100 tests FC FC PK-AB B PK-AB F 100 µg FC, FST (AI) PK-AB µg FC, FST (BL) PK-AB tests 100 tests FC FC PK-AB B PK-AB F 100 µg WB, IHC PK-AB µg WB PK-AB ARF-BP1 antibody Rabbit Anti-Human/Mouse/Rat ARF-Binding Protein µg WB PK-AB ARMER / ARL6IP / AIP1 (CT) antibody Rabbit Anti-Human/Mouse ARMER (Inhibitor of Apoptosis) 100 µg WB, IHC PK-AB ARTS antibody Rabbit Anti-Human ARTS (Apoptosis Related Protein) 100 µg WB PK-AB ASC antibody ASK1 / MAPKKK5 (C2) antibody ASK1 / MAPKKK5 (CT) antibody ASK1 / MAPKKK5 (NT) antibody Aven (CT) antibody Aven (NT) antibody Bad / Bcl-2-like 8 antibody BAG-1 / RAP46 (CT) antibody BAG-1 / RAP46 (NT) antibody Bak (NT) antibody Rabbit Anti-Human ASC (Novel CARD-Containing Adaptor Protein) Rabbit Anti-Human/Mouse ASK1 (MAPKKK5, Apoptosis Signal Regulating Kinase, C2) Rabbit Anti-Human ASK1 (MAPKKK5, Apoptosis Signal Regulating Kinase, CT) Rabbit Anti-Human/Mouse ASK1 (MAPKKK5, Apoptosis Signal Regulating Kinase, NT) Rabbit Anti-Human/Mouse/Rat Aven (Apoptosis Caspase Activation Inhibitor, CT) Rabbit Anti-Human/Mouse/Rat Aven (Apoptosis Caspase Activation Inhibitor, NT) Rabbit Anti-Human/Mouse/Rat Bad (Bcl-2 Family Member) Rabbit Anti-Human/Mouse/Rat Bcl-2 Binding Athanogene 1 (RAP46, CT) Rabbit Anti-Human/Mouse Bcl-2 Binding Athanogene 1 (RAP46, NT) Rabbit Anti-Human/Mouse Bak (Bcl-2 Antagonist/Killer, NT) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

124 122 Product Name Product Description Size* Application** Catalog Number Bax (NT) antibody Rabbit Anti-Human Bax (Bcl-2 Associated X Protein, NT) 100 µg WB, IHC PK-AB Bax antibody (mab) Bax antibody (mab) Mouse Anti-Human Bax (Bcl-2 Associated X-Protein; clone 2D2) Mouse Anti-Human/Mouse/Rat Bax (Bcl-2 Associated X-Protein; clone 6A7) 100 µg E, IP, WB PK-AB µg E, IP, WB PK-AB Bcl-2 (IN) antibody Rabbit Anti-Human B-cell Lymphoma/Leukemia 2 (IN) 100 µg WB PK-AB Bcl-2 (NT) antibody Rabbit Anti-Human/Mouse B-cell Lymphoma/ Leukemia 2 (NT) Bcl-2 antibody (mab) Mouse Anti-Human B-cell Lymphoma/Leukemia 2 (clone 8C8) Bcl-10 / CIPER / CLAP (NT) antibody Bcl-B / Bcl-2L10 antibody Bcl-G / Bcl-2-like 14 (CT) antibody Bcl-rambo / Mil1 (IN) antibody Rabbit Anti-Human B-cell Lymphoma/Leukemia 10 (Involved in Apoptosis and Tumor Genesis) Rabbit Anti-Human Bcl-B (Member of the Bcl-2 Family) Rabbit Anti-Human/Mouse/Rat Bcl-G (Member of the Bcl-2 Family) Rabbit Anti-Human/Mouse/Rat Bcl-rambo (Mil1, Member of the Bcl-2 Family, IN) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB Bcl-xL antibody Rabbit Anti-Human Bcl-xL (Member of the Bcl-2 Family) 100 µg WB PK-AB Beclin-1 (CT) antibody Beclin-1 (NT) antibody Bfl-1 (CT) antibody Bfl-1 (NT) antibody Bid (CT) antibody Bid (IN) antibody Rabbit Anti-Human/Mouse Beclin-1 (Autophagy Related Protein, CT) Rabbit Anti-Human/Mouse/Rat Beclin-1 (Autophagy Related Protein, NT) Rabbit Anti-Human/Mouse Bfl-1 (Bcl-2 Related Protein A1, CT) Rabbit Anti-Human/Mouse/Rat Bfl-1 (Bcl-2 Related Protein A1, NT) Rabbit Anti-Human/Mouse Bid (Member of the Bcl-2 Family, CT) Rabbit Anti-Human/Mouse Bid (Member of the Bcl-2 Family, IN) Bif antibody Rabbit Anti-Human/Mouse Bax Interacting Factor 1 (Endophilin B1) Bik antibody Bim / BOD (IN) antibody Bim / BOD (IN2) antibody Bit1 (IN) antibody Bit1 (NT) antibody Rabbit Anti-Human/Mouse Bcl-2 Interacting Killer (Apoptosis Inducer NBK) Rabbit Anti-Human/Mouse/Rat Bim (BOD, Bcl-2 Interacting Mediator of Cell Death, IN) Rabbit Anti-Human/Mouse/Rat Bim (BOD, Bcl-2 Interacting Mediator of Cell Death, IN2) Rabbit Anti-Human/Mouse Bcl-2 Inhibitor of Transcription 1 (IN) Rabbit Anti-Human/Mouse/Rat Bcl-2 Inhibitor of Transcription 1 (NT) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Bmf (CT) antibody Rabbit Anti-Human Bmf (Member of the Bcl-2 Family, CT) 100 µg WB PK-AB Bmf (NT) antibody Bnip3L / Nix (IN) antibody Rabbit Anti-Human/Mouse Bmf (Member of the Bcl-2 Family, NT) Rabbit Anti-Human Bnip3L (Nix, Member of the Bcl-2 Family, IN) 100 µg WB PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

125 123 Product Name Product Description Size* Application** Catalog Number CAD / DFF40 (CT) antibody CAD / DFF40 (I17) antibody Rabbit Anti-Mouse CAD (DFF40, Caspase Activated DNase, CT) Rabbit Anti-Mouse/Rat CAD (DFF40, Caspase Activated DNase, I17) 100 µg WB PK-AB µg WB, IHC PK-AB Calpain-2 antibody Rabbit Anti-Human/Mouse/Rat Calpain µl WB PK-AB Calpain-3 antibody Rabbit Anti-Human Calpain µl WB PK-AB Calpain-5 antibody Rabbit Anti-Human/Mouse/Rat Calpain µl WB PK-AB Calpain-6 antibody Rabbit Anti-Human/Mouse/Rat Calpain µl WB PK-AB Calpain-7 antibody Rabbit Anti-Human/Mouse/Rat Calpain µl WB PK-AB Calpain I antibody CARD8 / TUCAN antibody CARD9 antibody CARD10 / CARMA3 antibody CARMA1 antibody Caspase-1 / ICE antibody Caspase-1 / ICE (CT) antibody Caspase-1 / ICE (IN) antibody Caspase-2 / ICH-1 (NT) antibody Caspase-2 / ICH- 1 antibody (mab) Caspase-2 / ICH-1 (CT) antibody Rabbit Anti-Human/Mouse/Rat/Bovine/Pig/Rabbit Calpain I Rabbit Anti-Human CARD8 (Caspase Recruitment Domain Containing Protein 8) Rabbit Anti-Human CARD9 (Caspase Recruitment Domain Containing Protein 9) Rabbit Anti-Human/Mouse CARD10 (Caspase Recruitment Domain Containing Protein 10) Rabbit Anti-Human/Mouse CARMA1 (Lymphocyte Activating CARD Containing Protein) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Caspase µg WB, IP, IF PK-AB Rabbit Anti-Human Caspase 1 (ICE, Inflammatory Subfamily, CT) Rabbit Anti-Human Caspase 1 (ICE, Inflammatory Subfamily, IN) Rabbit Anti-Human/Mouse Caspase 2 (ICH-1, Nedd2, Induced by ER Stress & DNA Damage, NT) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB Mouse Anti-Human Caspase-2 (clone B22) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Caspase 2 (CT) 100 µg WB PK-AB Caspase-3 antibody (mab) Mouse Anti-Human/Mouse Caspase-3 (clone C33) 100 µg WB PK-AB Caspase-3 (active) antibody Rabbit Anti-Human/Mouse/Rat Caspase 3 (active) 100 µg WB, IHC PK-AB Caspase-3 antibody Rabbit Anti-Human/Mouse/Rat Caspase µg WB PK-AB Caspase-4 / ICH-2 antibody (mab) Caspase-4 / ICH-2 (IN) antibody Caspase-4 / ICH-2 (NT) antibody Caspase-5 / ICH-3 (IN) antibody Caspase-5 / ICH-3 (NT) antibody Caspase-5 / ICH3-3 antibody Mouse Anti-Human Caspase-4 (clone D44) 100 µg WB PK-AB Rabbit Anti-Human Caspase 2 (ICH-2, Induced by ER Stress, IN) Rabbit Anti-Human Caspase 2 (ICH-2, Induced by ER Stress, NT) Rabbit Anti-Human Caspase 5 (ICH-3, Inflammatory Subfamily, IN) Rabbit Anti-Human Caspase 5 (ICH-3, Inflammatory Subfamily, NT) Rabbit Anti-Human/Mouse/Rat/Rabbit/Monkey/ Bovine/Hamster Caspase µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

126 124 Product Name Product Description Size* Application** Catalog Number Caspase-6 (active) antibody Rabbit Anti-Human/Mouse/Rat Caspase 6 (active) 100 µg WB PK-AB Caspase-6 antibody Rabbit Anti-Human/Mouse/Rat Caspase µg WB PK-AB Caspase-6 / Mch2 (IN) antibody Caspase-6 / Mch2 (NT) antibody Caspase-7 antibody (mab) Caspase-7 / ICELAP3 (CT) antibody Caspase-7 / ICELAP3 (NT) antibody Rabbit Anti-Human Caspase 6 (Mch2, Executioner Caspase, IN) Rabbit Anti-Human Caspase 6 (Mch2, Executioner Caspase, NT) Mouse Anti-Human/Mouse/Rat Caspase-7 (clone B4-G2) Rabbit Anti-Human/Mouse/Rat Caspase 7 (ICELAP3, Executioner Caspase, CT) Rabbit Anti-Human/Mouse/Rat Caspase 7 (ICELAP3, Executioner Caspase, NT) 100 µg WB PK-AB µg WB, IHC PK-AB µg WB, IP PK-AB µg WB, IHC PK-AB µg WB PK-AB Caspase-8 antibody (mab) Mouse Anti-Human Caspase-8 (FLICE, clone C51S) 100 µg WB, IP PK-AB Caspase-8 / FLICE antibody (mab) Caspase-8 (active) antibody (mab) Caspase-8 (active) antibody Caspase-8 / FLICE (CT) antibody Caspase-8 / FLICE (IN) antibody Mouse Anti-Human Caspase 8 (FLICE, clone ) 100 µg E PK-AB Mouse Anti-Human Caspase-8 (active; clone C502S) 100 µg WB PK-AB Rabbit Anti-Mouse/Rat Caspase 8 (FLICE, active) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Caspase 8 (FLICE, Initiator Caspase, CT) Rabbit Anti-Human/Mouse Caspase 8 (FLICE, Initiator Caspase, IN) 100 µg WB, IHC PK-AB µg WB PK-AB Caspase-8 antibody Rabbit Anti-Human Caspase 8 (FLICE) 100 µg WB, IHC PK-AB Caspase-8 antibody Caspase-9 / Apaf-3 antibody Caspase-9 (active) antibody Caspase-9 (active) antibody Caspase-9 / Apaf-3 (IN1) antibody Caspase-9 / Apaf-3 (IN2) antibody Caspase-9 / Apaf-3 antibody Caspase-9 / Apaf-3 antibody Caspase-9 / Apaf-3 antibody Caspase-10 / FLICE2 (CT) antibody Rabbit Anti-Human/Mouse/Rat/Rabbit/Monkey/ Bovine Caspase 8 (FLICE) 100 µg WB PK-AB Rabbit Anti-Human Caspase 9 (Apaf-3) 100 µg WB, IP PK-AB Rabbit Anti-Human Caspase 9 (active) 100 µg WB, IP, IHC PK-AB Rabbit Anti-Rat Caspase 9 (active) 100 µg WB, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Caspase 9 (Apaf-3, IN1) 100 µg WB PK-AB Rabbit Anti-Human Caspase 9 (Apaf-3, IN2) 100 µg WB, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Caspase 9 (Apaf-3) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat/Hamster/Monkey/ Rabbit/Dog/Pig/Bovine Caspase 9 (Apaf-3) 100 µg WB PK-AB Rabbit Anti-Mouse/Rat Caspase 9 (Apaf-3) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Caspase 10 (FLICE2, CT) 100 µg WB PK-AB Caspase-11 antibody Rabbit Anti-Mouse/Rat Caspase µg WB PK-AB Caspase-12 (IN) antibody Rabbit Anti-Human/Mouse/Rat Caspase 12 (IN) 100 µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

127 125 Product Name Product Description Size* Application** Catalog Number Caspase-12 (lrg) antibody Caspase-12 (NT) antibody Caspase-12 (sml) antibody Caspase-12 antibody Rabbit Anti-Human/Mouse/Rat Caspase 12 (large) 100 µg WB, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Caspase 12 (NT) 100 µg WB, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Caspase 12 (small) 100 µg WB PK-AB Rabbit Anti-Mouse/Rat Caspase µg WB PK-AB Caspase-13 / ERICE antibody Caspase-13 / ERICE (CT) antibody Caspase-14 / MICE antibody Caspase-14 antibody (mab) ciap (CT) antibody CIDE-A (CT) antibody CIDE-A (CT) antibody CIDE-B (C2) antibody CIDE-B (CT) antibody CIDE-B (IN) antibody Cytochrome c antibody (mab) Cytochrome c antibody (mab) Cytochrome c antibody Cytochrome c antibody Cytochrome c antibody Cytochrome p450 antibody DAD1 antibody DAPK2 antibody Daxx / DAP-6 (CT) antibody DcR1 / TRAIL-R3 (ED) antibody Rabbit Anti-Human/Mouse/Rat Caspase 13 (Evolutionarily Related ICE) Rabbit Anti-Human/Mouse/Rat Caspase 13 (Evolutionarily Related ICE, CT) Rabbit Anti-Human/Mouse/Rat Caspase 14 (Mini Interleukin-1 Converting Enzyme) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB Mouse Anti-Mouse Caspase-14 (clone 80A1585) 100 µg WB PK-AB Rabbit Anti-Human/Mouse ciap (Cellular Inhibitor of Apoptosis, CT) Rabbit Anti-Human Cell Death Inducing DFF-Like Effector A (CT) Rabbit Anti-Mouse Cell Death Inducing DFF-Like Effector A (CT) Rabbit Anti-Human/Mouse/Rat Cell death-inducing DFF-like effector B (C2) Rabbit Anti-Mouse Cell Death Inducing DFF-Like Effector B (CT) Rabbit Anti-Human/Mouse/Rat Cell death-inducing DFF-like effector B (IN) Mouse Anti-Human Cytochrome c (clone 2G8) 100 µg E, FC (IS), FST (BL) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB PK-AB Mouse Anti-Human/Mouse/Rat Cytochrome c 100 µg WB, IP PK-AB Rabbit Anti-Human/Mouse/Rat/Bovine/Chicken/Dog Cytochrome c 100 µg WB PK-AB Rabbit Anti-Rat Cytochrome c 100 µg WB PK-AB Rabbit Anti-Mouse/Bovine Cytochrome c 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat/Dog/Horse Cytochrome P450 Rabbit Anti-Human/Mouse DAD1 (Defender Against Apoptotic Death) Rabbit Anti-Human/Mouse/Rat Death Associated Protein Kinase 2 Rabbit Anti-Human/Mouse Daxx (Death Associated Protein, Adaptor Molecule, CT) Rabbit Anti-Human/Mouse/Rat DcR1 (TRAIL-R3, Decoy Receptor 1 for TRAIL, ED) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

128 126 Product Name Product Description Size* Application** Catalog Number DcR1 / TRAIL-R3 (ED2) antibody DcR2 / TRAIL-R4 (ID) antibody DcR3 / TR6 (NT) antibody DEDAF antibody DEDD2 / FLAME-3 antibody DFF40 / CAD (I18) antibody DFF40 / CAD (IN) antibody DFF40 / CAD (NT) antibody DFF45 / ICAD (CT) antibody DFF45 / ICAD (NT) antibody DNase II (CT) antibody DR3 / Apo-3 (CT) antibody DR3 / Apo-3 (ED) antibody DR3 / Apo-3 (NT) antibody DR4 / TRAIL-R1 (CT) antibody DR4 / TRAIL-R1 (NT) antibody DR5 / TRAIL-R2 antibody Rabbit Anti-Human/Mouse/Rat DcR1 (TRAIL-R3, Decoy Receptor 1 for TRAIL, ED2) Rabbit Anti-Human/Mouse/Rat DcR2 (TRAIL-R4, Decoy Receptor 2 for TRAIL, IN) Rabbit Anti-Human/Mouse/Rat DcR3 (TR6, Decoy Receptor for FASL and LIGHT) Rabbit Anti-Human/Mouse/Rat DED Associated Factor (Novel Regulator of Apoptosis) Rabbit Anti-Human/Mouse/Rat DNA Binding Death Effector Domain Containing Protein 2 (FLAME-3) Rabbit Anti-Human/Mouse/Rat DNA Fragmentation Factor 40 (Caspase Activated DNase, I18) Rabbis Anti-Human DNA Fragmentation Factor 40 (Caspase Activated DNase, IN) Rabbit Anti-Human DNA Fragmentation Factor 40 (Caspase Activated DNase, NT) Rabbit Anti-Human DNA Fragmentation Factor 45 (Inhibitor of CAD, CT) Rabbit Anti-Human DNA Fragmentation Factor 45 (Inhibitor of CAD, NT) Rabbit Anti-Human DNase II (Involved in Apoptosis, CT) Rabbit Anti-Human/Mouse/Rat Death Receptor 3 (Apo-3, CT) Rabbit Anti-Human/Mouse Death Receptor 3 (Apo-3, ED) Rabbit Anti-Human/Mouse Death Receptor 3 (Apo-3, NT) 100 µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Rabbit Anti-Human Death Receptor 4 (TRAIL-R1, CT) 100 µg WB PK-AB Rabbit Anti-Human Death Receptor 4 (TRAIL-R1, NT) 100 µg WB PK-AB Rabbit Anti-Human/Mouse Death Receptor µg WB PK-AB DR6 (NT) antibody Rabbit Anti-Human Death Receptor 6 (NT) 100 µg WB PK-AB DRAK1 (NT) antibody DRAK2 (CT) antibody DRAM (CT) antibody DRAM (NT) antibody Rabbit Anti-Human DRAK1 (DAPK Related Apoptosis Inducing Kinase 1, NT) Rabbit Anti-Human DRAK2 (DAPK Related Apoptosis Inducing Kinase 2, CT) Rabbit Anti-Human/Mouse/Rat Damage Regulated Autophagy Modulator (CT) Rabbit Anti-Human/Mouse/Rat Damage Regulated Autophagy Modulator (NT) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB EndoG antibody Rabbit Anti-Human/Mouse/Rat Endonuclease G 100 µg WB, IHC PK-AB F1Aa (CT) antibody FAF1 (CT) antibody Rabbit Anti-Human/Mouse/Rat F1Aa (Novel Death Receptor Binding Protein, CT) Rabbit Anti-Human/Mouse/Rat FAF1 (Fas-associated Protein 1, CT) 100 µg WB PK-AB µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

129 127 Product Name Product Description Size* Application** Catalog Number FAIM antibody Rabbit Anti-Human/Mouse FAIM (Apoptosis Inhibitor) 100 µg WB PK-AB Fas / CD95 antibody (mab) Fas / CD95 antibody (mab) Fas / CD95 antibody (mab) Fas-1 / Apo1 antibody Fas Ligand antibody (mab) Fas Ligand antibody (mab), biotin Mouse Anti-Fas (CD95, clone NYRhFAS) 500 µg E, FST (BL/A) PK-AB Mouse Anti-Fas (CD95, clone NYRhFAS) 500 µg FST (BL) PK-AB Mouse Anti-Human Fas (CD95, clone 12J3) 100 µg WB, IHC (P) PK-AB Rabbit Anti-Human/Mouse/Rat Fas-1 (Apo-1) 100 µg WB PK-AB Rat Anti-Human Fas Ligand (clone H-11) 100 µg FC, IHC, IP PK-AB Rat Anti-Human Fas Ligand (clone H-11; biotinylated) 100 µg FC PK-AB B Fas Ligand antibody (mab), FITC Rat Anti-Human Fas Ligand (clone H-11; FITClabeled) 100 µg FC PK-AB F Fas Ligand antibody (mab) Mouse Anti-Human Fas Ligand (clone 14C2) 100 µg FC, FST (BL) PK-AB FLASH (CT) antibody FLIP / I-FLICE (CT) antibody FLIP / I-FLICE (CT) antibody FLIP / I-FLICE (NT) antibody Rabbit Anti-Human FLICE Associated Huge Protein (Homolog of CED4, CT) Rabbit Anti-Human/Mouse/Rat FLICE Inhibitory Protein (I-FLICE, CT) 100 µg WB PK-AB µg WB PK-AB Rabbit Anti-Mouse FLICE Inhibitory Protein (CT) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat FLICE Inhibitory Protein (I-FLICE, NT) 100 µg WB PK-AB FLIPs (CT) antibody Rabbit Anti-Human FLICE Inhibitory Protein (CT) 100 µg WB, IHC PK-AB Fn14 / TNFRSF12 antibody HAT-1 antibody Rabbit Anti-Human Fn14 (TNFRSF12, Fibroblast Growth Factor Inducible 14, TWEAK Receptor) Rabbit Anti-Human/Mouse/Rat Histone Acetyltransferase µg WB PK-AB µg WB PK-AB HAT-2 antibody Rabbit Anti-Human Histone Acetyltransferase µg WB PK-AB HAT-3 antibody Rabbit Anti-Human Histone Acetyltransferase µg WB PK-AB HCAM Polyclonal Ab Rabbit Anti-Human/Mouse/Rat Homing Cell Adhesion Molecule (CD44) 100 µg WB, IP, IHC PK-AB HDAC1 antibody Rabbit Anti-Human/Mouse/Rat Histone Deacetylase µg WB, IP, IHC PK-AB HDAC2 antibody HDAC3 antibody Rabbit Anti-Human/Mouse/Rat/Chicken/Hamster/ Dog Histone Deacetylase 2 Rabbit Anti-Human/Mouse/Rat/Chicken Histone Deacetylase µg WB, IHC PK-AB µg WB, IHC PK-AB HDAC4 antibody Rabbit Anti-Human/Chicken Histone Deacetylase µg WB PK-AB C HDAC4 antibody Rabbit Anti-Human/Mouse/Rat Histone Deacetylase µg WB PK-AB HDAC5 antibody Rabbit Anti-Human/Mouse/Rat Histone Deacetylase µg WB, IP, IHC PK-AB HDAC6 antibody Rabbit Anti-Human/Mouse/Rat Histone Deacetylase µg WB, IHC PK-AB HDAC7 antibody Rabbit Anti-Human/Mouse/Rat Histone Deacetylase µg WB, IP, IHC PK-AB HDAC8 antibody Rabbit Anti-Human/Mouse/Rat Histone Deacetylase µg WB, IP, IHC PK-AB HDAC9 antibody Rabbit Anti-Human/Mouse/Rat Histone Deacetylase µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

130 128 Product Name Product Description Size* Application** Catalog Number HDAC10 antibody Rabbit Anti-Human/Mouse/Rat Histone Deacetylase µg WB PK-AB HDAC11 antibody Rabbit Anti-Human/Mouse/Rat Histone Deacetylase µg WB PK-AB ICAD / DFF45 (CT) antibody ICAD / DFF45 (NT) antibody Rabbit Anti-Mouse Inhibitor of CAD (DFF45, CT) 100 µg WB PK-AB Rabbit Anti-Mouse Inhibitor of CAD (DFF45, NT) 100 µg WB, IHC PK-AB IEX-1 antibody Rabbit Anti-Human/Mouse IEX-1 (Stress-inducible Gene Induced by Ionizing Radiation) ILP-2 antibody Rabbit Anti-Human/Mouse/Rat IAP-like Protein 2 (Member in IAP Family) ILPIP (CT) antibody ILPIP (IN) antibody Ipaf / CARD12 antibody LAMP-1 antibody LAMP-2 antibody Rabbit Anti-Human IAP-like Protein Interacting Protein (CT) Rabbit Anti-Human/Mouse/Rat IAP-like Protein Interacting Protein (IN) Rabbit Anti-Human Ipaf (ICE Protease Activating Factor, CARD12) Rabbit Anti-Human/Mouse/Rat Lysosome Associated Membrane Glycoprotein 1 (Autophagy Related Protein) Rabbit Anti-Human/Mouse Lysosome Associated Membrane Glycoprotein 2 (Autophagy Related Protein) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB LFG antibody Rabbit Anti-Human/Mouse/Rat LFG (Apoptosis Inhibitor) 100 µg WB, IHC PK-AB Livin / KIAP antibody Rabbit Anti-Human Livin (KIAP, Novel Member in IAP Family) 100 µg WB PK-AB MAP1 (IN) antibody Rabbit Anti-Human/Mouse Modulator of Apoptosis 1 (IN) 100 µg WB PK-AB MCG10 antibody Mcl-1 (CT) antibody Mcl-1 (IN) antibody Mcl-1 (NT) antibody NADE antibody NAIP (CT) antibody NALP1 / CARD7 / DEF- CAP antibody NALP2 / PYPAF2 antibody NOD2 / CARD15 (CT) antibody NOD2 / CARD15 (NT) antibody Nop30 antibody Noxa / PMAIP1 / APR antibody Rabbit Anti-Human MCG10 (Member of the KH- Domain RNA-binding Protein Family) Rabbit Anti-Human/Mouse/Rat Mcl-1 (Bcl-2 Protein Family Member, CT) Rabbit Anti-Human Mcl-1 (Bcl-2 Protein Family Member, IN) Rabbit Anti-Human Mcl-1 (Bcl-2 Protein Family Member, NT) Rabbit Anti-Human/Mouse NADE (Novel Apoptosis Related Protein) Rabbit Anti-Human Neuronal Apoptosis Inhibitor Protein (CT) Rabbit Anti-Human NALP1 (CARD7, DEFCAP, Caspase Interacting Protein) Rabbit Anti-Human/Mouse/Rat NALP2 (PYPAF2, Caspase Interacting Protein) Rabbit Anti-Human Nucleotide-binding Oligomerization Domain Protein 2 (CARD15, CT) Rabbit Anti-Human Nucleotide-binding Oligomerization Domain Protein 2 (CARD15, NT) Rabbit Anti-Human/Mouse/Rat Nop30 (Alternate Splicing Isoform of ARC) Rabbit Anti-Human/Mouse/Rat Noxa (PMAIP1, APR, p53 Target Gene) 100 µg WB, ICC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

131 129 Product Name Product Description Size* Application** Catalog Number OMI / Htr2A antibody OMI / HtrA2 (C2) antibody Rabbit Anti-Human OMI (HtrA2, IAP Inhibitor) 100 µg WB PK-AB Rabbit Anti-Human OMI (HtrA2, IAP Inhibitor, C2) 100 µg WB PK-AB PDCD1 (CT) antibody PDCD1 (IN) antibody PDCD4 (CT) antibody PDL-1 antibody PDL-2 antibody PHAP (CT) antibody PHAP (NT) antibody PHAP I (CT) antibody PHAP I (IN) antibody PHAP III antibody PIKE (CT) antibody PIST / GOPC (CT) antibody PIST / GOPC (IN) antibody PUMA / bbc3 (CT) antibody PUMA / bbc3 (NT) antibody RAIDD / CRADD (CT) antibody RAIDD / CRADD (IN) antibody RICK / RIP2 (CT) antibody RICK / RIP2 (NT) antibody Rabbit Anti-Human/Mouse Programmed Cell Death 1 Protein (CT) Rabbit Anti-Human/Mouse/Rat Programmed Cell Death 1 Protein (IN) Rabbit Anti-Human/Mouse/Rat Programmed Cell Death Protein 4 (CT) Rabbit Anti-Human/Mouse/Rat PDL-1 (Novel Ligand of PDCD-1) Rabbit Anti-Human/Mouse/Rat PDL-2 (Novel Ligand of PDCD-1) Rabbit Anti-Human/Mouse/Rat PHAP (Regulator of Mitochondrion Apoptosis, CT) Rabbit Anti-Human/Mouse/Rat PHAP (Regulator of Mitochondrion Apoptosis, NT) Rabbit Anti-Human/Mouse/Rat PHAP I (Regulator of Mitochondrion Apoptosis, CT) Rabbit Anti-Human/Mouse PHAP I (Regulator of Mitochondrion Apoptosis, IN) Rabbit Anti-Human/Mouse/Rat PHAP III (Regulator of Mitochondrion Apoptosis) Rabbit Anti-Human/Mouse Phosphoinositide 3 Kinase Enhancer (Apoptosis Inhibitor, CT) Rabbit Anti-Human/Mouse/Rat PIST (GOPC, Autophagy Related Protein, CT) Rabbit Anti-Human/Mouse PIST (GOPC, Autophagy Related Protein, IN) Rabbit Anti-Human/Mouse PUMA (bbc3, Novel Member in Bcl-2 Family, CT) Rabbit Anti-Human PUMA (bbc3, Novel Member in Bcl-2 Family, NT) Rabbit Anti-Human RAIDD (CRADD, Adaptor Molecule with Death Domain, CT) Rabbit Anti-Human RAIDD (CRADD, Adaptor Molecule with Death Domain, IN) Rabbit Anti-Human RIP-Like Interacting CLARP Kinase (RIP2, CT) Rabbit Anti-Human/Mouse/Rat RIP-Like Interacting CLARP Kinase (RIP2, NT) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB RIP3 antibody Rabbit Anti-Mouse/Rat Receptor Interacting Protein µg WB PK-AB Smac / DIABLO (CT) antibody Smac / DIABLO (CT) antibody Rabbit Anti-Human/Mouse/Rat Smac (DIABLO, IAPs Binding Protein, CT) Rabbit Anti-Human/Mouse/Rat Smac (DIABLO, IAPs Binding Protein, CT) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

132 130 Product Name Product Description Size* Application** Catalog Number SODD (NT) antibody srank Ligand antibody ssdna (APOSTAIN) antibody (mab) Survivin / TIAP (CT) antibody Survivin / TIAP (CT) antibody Survivin / TIAP (NT) antibody TACE / ADAM17 (CT) antibody Rabbit Anti-Human/Mouse/Rat Silencer of Death Domains (NT) Rabbit Anti-Human/Mouse/Rat srank Ligand (TNFSF11, TRANCE, OPGL) Mouse Anti-Human/Mouse/Rat ssdna (APOSTAIN; clone F7-26) Rabbit Anti-Human Survivin (TIAP, Inhibitor of Apoptosis, CT) Rabbit Anti-Mouse Survivin (TIAP, Inhibitor of Apoptosis, CT) Rabbit Anti-Human Survivin (TIAP, Inhibitor of Apoptosis, NT) Rabbit Anti-Human/Mouse/Rat TNF-alpha Converting Enzyme (ADAM17, CT) 100 µg WB PK-AB µg WB PK-AB µg IHC (P) PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB TACI antibody Rabbit Anti-Human TACI (Receptor for BAFF & APRIL) 100 µg WB, IHC PK-AB TCCR (CT) antibody TCCR (NT) antibody TIGAR (IN1) antibody TIGAR (IN2) antibody TL1A (NT) antibody Rabbit Anti-Human T-cell Cytokine Receptor (CT) 100 µg WB PK-AB Rabbit Anti-Human T-cell Cytokine Receptor (NT) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat TIGAR (p53-induced Glycolysis and Apoptosis Regulator, IN1) Rabbit Anti-Human/Mouse TIGAR (p53-induced Glycolysis and Apoptosis Regulator, IN2) Rabbit Anti-Human/Mouse/Rat TNF-like 1A (DR3 Ligand, NT) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB TL1A antibody Rabbit Anti-Human/Mouse TNF-like 1A (DR3 Ligand) 100 µg WB PK-AB Toso / FAIM3 antibody TRAIL / Apo-2L (CT) antibody TWEAK antibody VE-Cadherin antibody VE-Cadherin antibody, biotin VE-Cadherin antibody, FITC XAF-1 (CT) antibody XAF-1 (IN) antibody XIAP (CT) antibody ZIPK (IN) antibody Rabbit Anti-Human Toso (Fas Apoptotic Inhibitory Molecule 3) Rabbit Anti-Human TNF Related Apoptosis Inducing Ligand (Apo-2L, Ligand for DR4 & DR5, CT) Rabbit Anti-Human/Mouse/Rat TNF-like Weak Inducer of Apoptosis (TNF-alpha Related Protein) Rabbit Anti-Human VE-Cadherin 100 µg E, FC, FST (BL), IHC Rabbit Anti-Human VE-Cadherin (Biotin) 100 tests Rabbit Anti-Human VE-Cadherin (FITC) 100 tests Rabbit Anti-Human/Mouse XIAP Associated Factor 1 (Inhibitor of XIAP, CT) Rabbit Anti-Human XIAP Associated Factor 1 (Inhibitor of XIAP, IN) Rabbit Anti-Human/Mouse X-linked Inhibitor of Apoptosis Protein (CT) Rabbit Anti-Human/Mouse/Rat ZIP Kinase (Apoptosis Inducing Kinase, IN) 100 µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB FC FC PK-AB PK-AB B PK-AB F 100 µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

133 131 n p53 Network (Host: rabbit) Product Name Product Description Size* Application** Catalog Number Chk2 (NT) antibody MTBP (IN2) antibody Rabbit Anti-Human/Mouse/Rat Chk2 (Novel Check Point Kinase, NT) Rabbit Anti-Human MTBP (MDM2 Binding Protein, IN2) 100 µg WB PK-AB µg WB PK-AB p53 antibody (mab) Mouse Anti-p53 (clone PP53SHG) 100 µg WB, IP PK-AB p53 antibody Rabbit Anti-Human/Mouse/Rat/Dog/Rabbit p µg WB p53dinp1 / SIP antibody p53r2 (NT) antibody PARC antibody PERP antibody PID / MTA2 antibody Rabbit Anti-Human/Mouse/Rat p53dinp1 (SIP, Apoptosis Associated p53 Target) Rabbit Anti-Human/Mouse/Rat p53r2 (Novel Ribonucleotide Reductase, NT) Rabbit Anti-Human/Mouse PARC (p53 Associated Parkin-Like Cytoplasmic Protein) Rabbit Anti-Human PERP (Mediator of p53 Induced Apoptosis) Rabbit Anti-Human/Mouse/Rat PID (MTA2, Involved in p53 Deacetylation) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB n Neurobiology (Host: rabbit) Product Name Product Description Size* Application** Catalog Number APH1 (IN) antibody APH1 (NT) antibody APP (CT) antibody APP / AβNT antibody BACE / Asp2 (CT) antibody BACE2 / Asp1 (CT) antibody BACE2 / Asp1 (NT) antibody Rabbit Anti-Human/Mouse/Rat APH1 (Component of the Presenilin Complex, IN) Rabbit Anti-Human/Mouse APH1 (Component of the Presenilin Complex, NT) Rabbit Anti-Human/Mouse/Rat Amyloid Protein Precursor (CT) Rabbit Anti-Human/Mouse/Rat Amyloid Protein Precursor (AbNT) Rabbit Anti-Human/Mouse beta-site APP Cleaving Enzyme (Asp2, CT) Rabbit Anti-Human/Mouse beta-site APP Cleaving Enzyme 2 (Asp1, CT) Rabbit Anti-Human/Mouse/Rat beta-site APP Cleaving Enzyme 2 (Asp1, NT) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB BAP3 (NT) antibody Rabbit Anti-Human BAI1 Associated Protein 3 (NT) 100 µg WB PK-AB BAP3 (NT2) antibody BICD1 (CT) antibody BMI-1 (IN) antibody Rabbit Anti-Human BAI1 Associated Protein 3 (NT2) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat BICD1 (Bicaudal D homolog 1, CT) Rabbit Anti-Human/Mouse/Rat BMI-1 (Polycomb Group RING Finger Protein 4, RNF51, IN) 100 µg WB PK-AB µg WB PK-AB BRAL1 antibody Rabbit Anti-Human/Mouse/Rat Brain-link Protein µg WB PK-AB BRSK1 (CT) antibody BRSK1 (IN) antibody Rabbit Anti-Human/Mouse/Rat BR Serine-Threonine Kinase 1 (CT) Rabbit Anti-Human/Mouse/Rat BR Serine-Threonine Kinase 1 (IN) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

134 132 Product Name Product Description Size* Application** Catalog Number BRSK2 antibody CAPS1 (CT) antibody CAPS1 (NT) antibody CAPS2 (IN) antibody CDNF (CT) antibody CDNF (NT) antibody Clusterin / CLI / ApoJ antibody CRMP1 / DRP-1 (CT) antibody CRMP1 / DRP-1 (IN) antibody DISC1 (IN) antibody DISC1 (NT) antibody FEZ1 antibody FEZ2 (IN) antibody Rabbit Anti-Human/Mouse BR Serine-Threonine Kinase 2 Rabbit Anti-Human/Mouse/Rat CAPS1 (Calciumdependent Secretion Activator 1, CADPS1, CT) Rabbit Anti-Human/Mouse/Rat CAPS1 (Calciumdependent Secretion Activator 1, CADPS1, NT) Rabbit Anti-Human/Mouse/Rat CAPS2 (Calciumdependent Secretion Activator 2, CADPS2, IN) Rabbit Anti-Human/Mouse/Rat Conserved Dopamine Neurotrophic Factor (CT) Rabbit Anti-Human/Mouse/Rat Conserved Dopamine Neurotrophic Factor (NT) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB Rabbit Anti-Human Clusterin (Apolipoprotein J, CLI) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Collapsin Response Mediator Protein (DRP-1, Involved in NT3 Induced Neurite Outgrowth, CT) Rabbit Anti-Human/Mouse Collapsin Response Mediator Protein (DRP-1, Involved in NT3 Induced Neurite Outgrowth, IN) Rabbit Anti-Human/Mouse Disrupted in Schizophrenia 1 (IN) Rabbit Anti-Human/Mouse Disrupted in Schizophrenia 1 (NT) Rabbit Anti-Human/Mouse/Rat FEZ1 (Protein Involved in Axonal Guidance) Rabbit Anti-Human/Mouse/Rat Fasciculation and Elongation Protein zeta-2 (IN) 100 µg WB PK-AB µg WB PK-AB µg WB, ICC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB FEZ2 antibody Rabbit Anti-Human/Mouse/Rat Fasciculation and Elongation Protein zeta-2 (CT) 100 µg WB PK-AB GFRα-1 / GDNFRα (IN) antibody Rabbit Anti-Human/Mouse/Rat GDNF Receptor alpha-1 (IN) 100 µg WB PK-AB GFRα-2 / GDNFRβ (IN) antibody Rabbit Anti-Human/Mouse/Rat GDNF Receptor alpha-2 (IN) 100 µg WB PK-AB GFRα-3 / GDNFRγ (IN) antibody Grik1 (CT) antibody Rabbit Anti-Human/Mouse/Rat GDNF Receptor alpha-3 (IN) Rabbit Anti-Human/Mouse Grik1 (Glutamate Receptor 5, CT) 100 µg WB PK-AB µg WB PK-AB Grik1 (IN) antibody Rabbit Anti-Human/Mouse/Rat Grik1 (Glutamate Receptor 5, IN) 100 µg WB PK-AB Grik2 (C2) antibody Rabbit Anti-Human/Mouse/Rat Grik2 (Glutamate Receptor 5, C2) 100 µg WB PK-AB Grik3 antibody Rabbit Anti-Human/Mouse/Rat Grik3 (Glutamate receptor 5) 100 µg WB PK-AB Grik4 (CT) antibody Rabbit Anti-Human/Mouse/Rat Grik4 (The KA1 Subunit of Kainate-type Ionotropic Glutamate Receptors, CT) 100 µg WB PK-AB Grik4 (NT) antibody Rabbit Anti-Human/Mouse/Rat Grik4 (The KA1 Subunit of Kainate-type Ionotropic Glutamate Receptors, NT) 100 µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

135 133 Product Name Product Description Size* Application** Catalog Number Grik5 antibody HAP1 antibody KIF5 (CT) antibody KIF5 (IN) antibody Rabbit Anti-Human/Mouse/Rat Grik5 (Glutamate Receptor KA2 Subunit) Rabbit Anti-Human/Mouse/Rat Huntingtonassociated Protein 1 Rabbit Anti-Human/Mouse/Rat Kinesin Heavy Chain Isoform 5 (CT) Rabbit Anti-Human/Mouse/Rat Kinesin Heavy Chain Isoform 5 (IN) 100 µg WB PK-AB µg WB, IHC PK-AB µg WB, ICC PK-AB µg WB, ICC PK-AB KLHL1 antibody Rabbit Anti-Human/Rat Kelch-like 1 Protein 100 µg WB PK-AB LGI1 (IN) antibody LGI1 (NT) antibody LGI2 (IN) antibody LGI2 (NT) antibody LGI3 (IN) antibody LGI4 (CT) antibody LGI4 (IN) antibody Rabbit Anti-Human/Mouse/Rat Leucine-rich, Glioma Inactivated 1 (IN) Rabbit Anti-Human/Mouse/Rat LGI1 (Leucine-rich, Glioma Inactivated 1, Epitempin, EPT, NT) Rabbit Anti-Human/Mouse Leucine-rich, Glioma Inactivated 2 (IN) Rabbit Anti-Human/Mouse/Rat Leucine-rich, Glioma Inactivated 2 (NT) Rabbit Anti-Human/Mouse/Rat Leucine-rich, Glioma Inactivated 3 (IN) Rabbit Anti-Human/Mouse/Rat LGI4 (Leucine-rich, Glioma Inactivated 4) Rabbit Anti-Human/Mouse/Rat LGI4 (Leucine-rich, Glioma Inactivated 4) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB LIS1 antibody Rabbit Anti-Human/Mouse/Rat Lissencephaly µg WB PK-AB MANF (CT) antibody MANF (NT) antibody Neuritin antibody Neurotrypsin (NT) antibody Rabbit Anti-Human/Mouse/Rat Mesencephalic Astrocyte-derived Neurotrophic Factor (CT) Rabbit Anti-Human/Mouse/Rat Mesencephalic Astrocyte-derived Neurotrophic Factor (NT) Rabbit Anti-Human/Mouse Neuritin (Neurite-outgrowth Promoting Protein) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Neurotrypsin (NT) 100 µg WB, IHC PK-AB Neurturin antibody Rabbit Anti-Human Neurturin (Ligand for GFRa-2) 100 µg WB PK-AB Nhe-1 (CT) antibody Nhe-1 (IN) antibody Nicastrin (CT) antibody Nicastrin (IN) antibody Rabbit Anti-Human/Mouse/Rat Nhe-1 (Sodium-hydrogen Exchanger 1, CT) Rabbit Anti-Human/Mouse/Rat Nhe-1 (Sodium-hydrogen Exchanger 1, IN) Rabbit Anti-Human/Mouse/Rat Nicastrin (Anterior Pharynx Defective 2, APH-2, CT) Rabbit Anti-Human/Mouse/Rat Nicastrin (Anterior Pharynx Defective 2, APH-2, IN) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB NIK (CT) antibody Rabbit Anti-Human NF-kB Inducing Kinase (CT) 100 µg WB PK-AB NIPSNAP (CT) antibody NIPSNAP (IN) antibody NK3R (IN) antibody Rabbit Anti-Human/Mouse/Rat NIPSNAP (Non-neuronal SNAP25-like Protein, CT) Rabbit Anti-Human/Mouse/Rat NIPSNAP (Non-neuronal SNAP25-like Protein, IN) Rabbit Anti-Human/Mouse NK3R (Neurokinin B Receptor, IN) 100 µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

136 134 Product Name Product Description Size* Application** Catalog Number NogoA (IN) antibody Rabbit Anti-Human/Mouse/Rat NogoA (Myelin-associated Neurite-outgrowth Inhibitor, IN) 100 µg WB, IHC PK-AB NogoA (NT) antibody Rabbit Anti-Human/Mouse/Rat NogoA (Myelin-associated Neurite-outgrowth Inhibitor, NT) 100 µg WB, IHC PK-AB NPAS3 (IN) antibody Rabbit Anti-Human/Mouse/Rat Neuronal PAS Domain-containing Protein 3 (IN) 100 µg WB, IHC PK-AB NPAS3 (NT) antibody Rabbit Anti-Human/Mouse/Rat Neuronal PAS Domain-containing Protein 3 (NT) 100 µg WB, IHC PK-AB NPTX2 antibody Rabbit Anti-Human/Mouse/Rat NPTX2 (Neuronal Pentraxin 2, NP2, NARP) 100 µg WB PK-AB PEN2 (CT) antibody Rabbit Anti-Human/Mouse/Rat Presenilin Enhancer Protein 2 (gamma-secretase Subunit, CT) 100 µg WB PK-AB PEN2 (NT) antibody Rabbit Anti-Human/Mouse/Rat Presenilin Enhancer Protein 2 (gamma-secretase Subunit, NT) 100 µg WB PK-AB Presenilin-1 antibody Rabbit Anti-Human/Mouse/Rat Presenilin 1 (gamma-secretase Subunit) 100 µg WB, IHC PK-AB Rim2 antibody Rabbit Anti-Human/Mouse/Rat Rim2 (Rab3-interacting Molecule 2) 100 µg WB PK-AB Rim3 (NT) antibody Rabbit Anti-Human/Mouse Rab3-interacting Molecule 3 (NT) 100 µg WB PK-AB SAPAP1 antibody Rabbit Anti-Human/Mouse/Rat SAPAP1 (SAP90/PSD-95-associated Protein 1) 100 µg WB PK-AB SAPAP2 antibody Rabbit Anti-Human/Mouse/Rat SAPAP2 (SAP90/PSD-95-associated Protein 2) 100 µg WB PK-AB SAPAP3 antibody Rabbit Anti-Human/Mouse/Rat SAP90/PSD-95- associated Protein µg WB PK-AB SCRAPPER (CT) antibody Rabbit Anti-Human/Mouse/Rat SCRAPPER (F-box / Leucine-rich Repeat Protein 20, CT) 100 µg WB PK-AB SCRAPPER (NT) antibody Rabbit Anti-Human/Mouse/Rat SCRAPPER (F-box / Leucine-rich Repeat Protein 20, NT) 100 µg WB PK-AB Slitrk1 (CT) antibody Rabbit Anti-Human/Mouse/Rat Slitrk1 (SLIT and NTRK-like Family 1, CT) 100 µg WB PK-AB Slitrk1 (IN) antibody Rabbit Anti-Human/Mouse/Rat Slitrk1 (SLIT and NTRK-like Family 1, IN) 100 µg WB PK-AB Slitrk2 (CT) antibody Slitrk2 (IN) antibody Rabbit Anti-Human/Mouse/Rat Slitrk2 (SLIT and NTRK-like Family 2, CT) Rabbit Anti-Human/Mouse/Rat Slitrk2 (SLIT and NTRK-like Family 2, IN) 100 µg WB PK-AB µg WB PK-AB Slitrk3 (CT) antibody Rabbit Anti-Human/Mouse Slitrk3 (SLIT and NTRK-like Family 3, CT) 100 µg WB PK-AB Slitrk3 (IN) antibody Rabbit Anti-Human/Mouse Slitrk3 (SLIT and NTRK-like Family 3, IN) 100 µg WB PK-AB Slitrk4 (CT) antibody Rabbit Anti-Human/Mouse/Rat Slitrk4 (SLIT and NTRK-like Family 4, CT) 100 µg WB PK-AB Slitrk4 (NT) antibody Rabbit Anti-Human/Mouse/Rat Slitrk4 (SLIT and NTRK-like Family 4, NT) 100 µg WB PK-AB Slitrk5 (IN1) antibody Rabbit Anti-Human/Mouse/Rat Slitrk5 (SLIT and NTRK-like Family 5, IN1) 100 µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

137 135 Product Name Product Description Size* Application** Catalog Number Slitrk5 (IN2) antibody Slitrk6 (CT) antibody Slitrk6 (IN) antibody Syntaphilin (IN) antibody Syntaphilin (NT) antibody TDP43 (IN) antibody TDP43 (NT) antibody TMP21 (CT) antibody TMP21 (IN) antibody TOCA-1 (CT) antibody TOCA-1 (NT) antibody VGF antibody Rabbit Anti-Human/Mouse/Rat Slitrk5 (SLIT and NTRK-like Family 5, IN2) Rabbit Anti-Human/Mouse/Rat Slitrk6 (SLIT and NTRK-like Family 6, CT) Rabbit Anti-Human/Mouse/Rat Slitrk6 (SLIT and NTRK-like Family 6, IN) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Syntaphilin (SNPH, IN) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Syntaphilin (SNPH, NT) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat TAR DNA Binding Protein (IN) Rabbit Anti-Human/Mouse/Rat TAR DNA Binding Protein (NT) Rabbit Anti-Human/Mouse/Rat TMP21 (Component of the Presenilin Complex, CT) Rabbit Anti-Human/Mouse/Rat TMP21 (Component of the Presenilin Complex, IN) Rabbit Anti-Human/Mouse/Rat Transducer of cdc42- dependent Actin Assembly 1 (CT) Rabbit Anti-Human/Mouse/Rat Transducer of cdc42- dependent Actin Assembly 1 (NT) Rabbit Anti-Human/Mouse/Rat VGF (Neurosecretory protein, nerve growth factor inducible) 100 µg WB, ICC PK-AB µg WB, ICC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB n Virology Product Name Product Description Size* Application** Catalog Number HIV and Chemokine Receptors (Host: rabbit) APOBEC3G (NT) antibody BANF1 (CT) antibody BANF1 (NT) antibody Bonzo / STRL33 (CT) antibody Bonzo / STRL33 (NT2) antibody Bst2 antibody (NT) CCR3 (CT) antibody CCR3 (NT) antibody Rabbit Anti-Human APOBEC3G (HIV Restriction Factor, NT) Rabbit Anti-Human/Mouse BANF1 (Barrier to Autointegration Factor, CT) Rabbit Anti-Human/Mouse/Rat BANF1 (Barrier to Autointegration Factor, NT) 100 µg WB, IHC PK-AB µg WB, IF PK-AB µg WB, IF PK-AB Rabbit Anti-Human Bonzo (STRL33, HIV Coreceptor, CT) 100 µg WB PK-AB Rabbit Anti-Human Bonzo (STRL33, HIV Coreceptor, CT2) Rabbit Anti-Human/Mouse Bst2 (Bone marrow stromal cell antigen 2, CD317) Rabbit Anti-Human/Mouse/Rat CC Chemokine Receptor 3 (HIV Receptor, CT) Rabbit Anti-Human/Mouse/Rat CC Chemokine Receptor 3 (HIV Receptor, NT) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

138 136 Product Name Product Description Size* Application** Catalog Number CCR5 (NT) antibody CCR8 (EL) antibody CX3CL1 / CX3C antibody CX3CR1 / V28 (EL) antibody CX3CR1 / V28 (NT) antibody CXCR4 (EL) antibody CXCR4 (NT) antibody CXCR4-Lo antibody DC-SIGN (CT) antibody DC-SIGN (ED) antibody DDX3 (IN) antibody DDX3 (NT) antibody Emerin antibody Eotaxin (CT) antibody GPR15 / BOB (NT) antibody Rabbit Anti-Human CC Chemokine Receptor 5 (HIV Receptor, NT) Rabbit Anti-Human CC Chemokine Receptor 8 (HIV Receptor, EL) Rabbit Anti-Human/Mouse CX3C-Chemokine Ligand 1 (CX3CR1 Ligand, Neurotactin, Fractalkine) Rabbit Anti-Human CX3C-Chemokine Receptor 1 (V28, HIV Receptor, EL) Rabbit Anti-Human/Mouse/Rat CX3C-Chemokine Receptor 1 (V28, HIV Receptor, NT) Rabbit Anti-Human/Mouse CX3C-Chemokine Receptor 4 (HIV Receptor, EL) Rabbit Anti-Human/Mouse CX3C-Chemokine Receptor 4 (HIV Receptor, NT) Rabbit Anti-Human CXCR4-Lo (Isoform a of HIV & Chemokine Receptor) Rabbit Anti-Human DC-SIGN (Novel HIV Binding Protein, CT) Rabbit Anti-Human DC-SIGN (Novel HIV Binding Protein, ED) Rabbit Anti-Human/Mouse/Rat Dead-box Polypeptide 3 X-linked (DBX, IN) Rabbit Anti-Human/Mouse Dead-box Polypeptide 3 X-linked (DBX, NT) Rabbit Anti-Human/Mouse/Rat Emerin (Serine-rich Nuclear Membrane Member of the Nuclear Lamina-associated Protein Family) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC, FACS PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB Rabbit Anti-Human Eotaxin (CCR3 Ligand, CT) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat G-Protein Coupled Receptor 15 (HIV Coreceptor) 100 µg WB PK-AB HIV-Igp24 antibody (mab) Mouse Anti-Human HIV-I Glycoprotein µg E PK-AB HIV-Igp41 antibody (mab) Mouse Anti-Human HIV-I Glycoprotein µg E PK-AB HIV-Igp41 antibody Rabbit Anti-Human HIV-I Glycoprotein 41 1 ml*** E PK-AB HIV-Igp120 antibody (mab) HIV-Igp120 antibody HIV-Igp120 (NT) antibody HIV-Igp120 antibody HIV-IIgp39 antibody (mab) HIV-IIgp39 antibody p53aip1 antibody Mouse Anti-Human HIV-I Glycoprotein 120 (PND) 500 µg E PK-AB Rabbit Anti-Human-HIV-I Glycoprotein ml WB, E PK-AB Rabbit Anti-Human HIV-1 Glycoprotein 120 (NT) 100 µg WB PK-AB Rabbit Anti-Human-HIV-I Glycoprotein 120 (purified) 100 µg WB, E PK-AB Mouse Anti-Human HIV-II Glycoprotein µg E PK-AB Rabbit Anti-Human HIV-II Glycoprotein 39 1 ml*** E PK-AB Rabbit Anti-Human p53-regulated Apoptosis Inducing Protein µg WB, ICC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

139 137 Product Name Product Description Size* Application** Catalog Number TRBP1 (IN) antibody TRIM5α (CT) antibody TRIM5α (IN1) antibody TRIM5α (IN2) antibody TRIM5γ antibody TRIM5δ antibody Herpes TNFRSF14 / HVEM (CT) antibody TNFRSF14 / HVEM (NT) antibody SARS and SARS Receptors ACE2 (CT) antibody ACE2 (IN1) antibody ACE2 (IN2) antibody ACE2 (NT) antibody SARS E (CT) antibody SARS E (NT) antibody SARS M (CT) antibody SARS M (NT) antibody SARS Spike antibody (mab) SARS Spike antibody SARS Spike (CT) antibody SARS Spike (IN1) antibody SARS Spike (IN2) antibody SARS Spike (IN3) antibody SARS Spike (NT) antibody Rabbit Anti-Human/Mouse TAR RNA binding protein 1 (IN) Rabbit Anti-Human TRIM5α (Retroviral Restriction Factor, CT) Rabbit Anti-Human TRIM5α (Retroviral Restriction Factor, IN1) Rabbit Anti-Human TRIM5α (Retroviral Restriction Factor, IN2) Rabbit Anti-Human TRIM5γ (Retroviral Restriction Factor) Rabbit Anti-Human TRIM5δ (Retroviral Restriction Factor) Rabbit Anti-Human TNFRSF14 (Herpes Virus Entry Mediator, CT) Rabbit Anti-Human TNFRSF14 (Herpes Virus Entry Mediator, NT) Rabbit Anti-Human Angiotensin Converting Enzyme 2 (SARS Receptor, CT) Rabbit Anti-Human/Mouse Angiotensin Converting Enzyme 2 (SARS Receptor, IN1) Rabbit Anti-Human Angiotensin Converting Enzyme 2 (SARS Receptor, IN2) Rabbit Anti-Human Angiotensin Converting Enzyme 2 (SARS Receptor, NT) 100 µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB Rabbit Anti-SARS Virus Envelope Protein (CT) 100 µg E PK-AB Rabbit Anti-SARS Virus Envelope Protein (CT) 100 µg E PK-AB Rabbit Anti-SARS Virus Membrane Protein (CT) 100 µg E PK-AB Rabbit Anti-SARS Virus Membrane Protein (NT) 100 µg E PK-AB Mouse Anti-SARS Virus Membrane Protein 100 µg WB PK-AB Rabbit Anti-SARS Virus Membrane Protein 100 µg WB PK-AB Rabbit Anti-SARS Virus Membrane Protein (CT) 100 µg E PK-AB Rabbit Anti-SARS Virus Membrane Protein (IN1) 100 µg E PK-AB Rabbit Anti-SARS Virus Membrane Protein (IN2) 100 µg E PK-AB Rabbit Anti-SARS Virus Membrane Protein (IN3) 100 µg E PK-AB Rabbit Anti-SARS Virus Membrane Protein (NT) 100 µg E PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

140 138 Product Name Product Description Size* Application** Catalog Number SARS-NP antibody (mab) Mouse Anti-SARS Nucleocapsid Protein 100 µg WB PK-AB SARS-NP antibody Rabbit Anti-SARS Nucleocapsid Protein 100 µg WB, CS PK-AB Anthrax (Host: rabbit) Anthrax EF antibody Rabbit Anti-Anthrax Edema Factor 100 µg E PK-AB Anthrax LF antibody Rabbit Anti-Anthrax Lethal Factor 200 µl *** WB PK-AB Anthrax LF (CT) antibody Anthrax LF (IN) antibody Rabbit Anti-Anthrax Lethal Factor (CT) 100 µg E PK-AB Rabbit Anti-Anthrax Lethal Factor (IN) 100 µg E PK-AB Anthrax PA antibody Rabbit Anti-Anthrax Protective Antigen 200 µl *** WB PK-AB Anthrax PA (CT) antibody Anthrax PA (IN) antibody Rabbit Anti-Anthrax Protective Antigen (CT) 100 µg E PK-AB Rabbit Anti-Anthrax Protective Antigen (IN) 100 µg E PK-AB ATR / TEM8 (CT) antibody ATR / TEM8 (IN) antibody West Nile Virus WNV Core (CT) antibody WNV Core (NT) antibody WNV Env (CT) antibody WNV Env (IN) antibody WNV Env (NT) antibody WNV Matrix (CT) antibody WNV Matrix (IN) antibody Influenza Influenza-A H1N1 antibody (mab) Influenza-A H3N2 antibody (mab) Influenza-A H5N1 antibody (mab) Influenza-B antibody (mab) Rabbit Anti-Human/Mouse/Rat Anthrax Receptor (TEM8, CT) Rabbit Anti-Human/Mouse/Rat Anthrax Receptor (TEM8, IN) 100 µg WB PK-AB µg WB, IHC PK-AB Rabbit Anti-West Nile Virus Core Protein (CT) 100 µg E PK-AB Rabbit Anti-West Nile Virus Core Protein (NT) 100 µg E PK-AB Rabbit Anti-West Nile Virus Envelope Protein (CT) 100 µg E PK-AB Rabbit Anti-West Nile Virus Envelope Protein (IN) 100 µg E PK-AB Rabbit Anti-West Nile Virus Envelope Protein (NT) 100 µg E PK-AB Rabbit Anti-West Nile Virus Matrix Protein (CT) 100 µg E PK-AB Rabbit Anti-West Nile Virus Matrix Protein (IN) 100 µg E PK-AB Mouse Anti-Influenza-A Hemagglutinin H1N1 (clone IA-H1N1) Mouse Anti-Influenza-A Hemagglutinin H3N2 (clone IA-H3N2) Mouse Anti-Influenza-A Hemagglutinin H5N1 (clone IA-H5N1) 50 µg WB, E PK-AB µg WB, E PK-AB µg WB, E PK-AB Mouse Anti-Influenza-B (clone IB-53-99) 50 µg WB, E PK-AB Avian Influenza AFHA-2 antibody Rabbit Anti-Avian Flu Hemagglutinin 2 (Avian Influenza H5N1) 100 µg E PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

141 139 Product Name Product Description Size* Application** Catalog Number AFHA-3 antibody Rabbit Anti-Avian Flu Hemagglutinin 3 (Avian Influenza H5N1) AFHA-4 antibody Rabbit Anti-Avian Flu Hemagglutinin 4 (Avian Influenza H5N1) 100 µg E PK-AB µg E PK-AB Avian Influenza A M2 antibody H5N1 Hemagglutinin (IN) antibody H5N1 Hemagglutinin (NT) antibody H5N1 Neuramidase (CT) antibody H5N1 Neuramidase (IN) antibody Rabbit Anti-Avian Influenza A M2 (H5N1 Membrane Ion Channel 2) 100 µg WB PK-AB Rabbit Anti-Avian Flu H5N1 Hemagglutinin (IN) 100 µg E PK-AB Rabbit Anti-Avian Flu H5N1 Hemagglutinin (NT) 100 µg E PK-AB Rabbit Anti-Avian Flu H5N1 Neuramidase (CT) 100 µg E PK-AB Rabbit Anti-Avian Flu H5N1 Neuramidase (IN) 100 µg E PK-AB H5N1 NS1 antibody Rabbit Anti-H5N1 Nonstructural Protein µg E PK-AB H5N1 NS2 / NEP antibody Hepatitis Rabbit Anti-H5N1 Nonstructural Protein 2 (Nuclear Export Protein) 100 µg E PK-AB HbsAgA antibody Rabbit Anti-Hepatitis B Surface Antigen A 1 ml E PK-AB HCVNS3 antibody (mab) Mouse Anti-HCV NS3 100 µg E PK-AB HDV antibody (mab) Mouse Anti-HDV 100 µg WB, IF PK-AB Hepatitis B antibody (mab) Mouse Anti-Hepatitis B (AD & AY Antigens) 500 µg E PK-AB Epstein-Barr Virus EBV (EBNA1) antibody (mab) Mouse Anti-Epstein-Barr Virus EBNA1 (clone P1B5AT) 50 µg WB, E PK-AB Dengue Virus Dengue type 2 antibody (mab) Virus-related Rift Valley Fever Virus (CT) antibody Rift Valley Fever Virus (IN) antibody UNG1 (CT) antibody UNG1 (NT) antibody UNG2 (IN) antibody UNG2 (NT) antibody Mouse Anti-Dengue Type 2 (Envelope) 500 µg IS PK-AB Rabbit Anti-Rift Valley Fever Virus (CT) 100 µg E PK-AB Rabbit Anti-Rift Valley Fever Virus (IN) 100 µg E PK-AB Rabbit Anti-Human/Mouse/Rat Uracil-DNA Glycosylase 1 (UDG1, CT) Rabbit Anti-Human/Mouse/Rat Uracil-DNA Glycosylase 1 (UDG1, NT) Rabbit Anti-Human/Mouse/Rat Uracil-DNA Glycosylase 2 (UDG2, IN) Rabbit Anti-Human/Mouse/Rat Uracil-DNA Glycosylase 2 (UDG2, NT) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

142 140 n Toll-like receptor related (Host: rabbit) Product Name Product Description Size* Application** Catalog Number BTK / ATK / BPK (NT) antibody ECSIT (CT) antibody MD-1 (IN) antibody MD-2 (IN) antibody RIG-1 antibody SARM (CT) antibody SIGIRR (CT) antibody Rabbit Anti-Human/Mouse Bruton's Tyrosine Kinase (ATK, BPK, NT) Rabbit Anti-Human/Mouse ECSIT (Intermediate in the TLR & Bmp Signaling Pathways, CT) Rabbit Anti-Human/Mouse/Rat MD-1 (Lymphocyte Antigen 86, IN) Rabbit Anti-Human/Mouse/Rat MD-2 (Toll-Like Receptor Adaptor Protein, IN) Rabbit Anti-Human/Mouse/Rat Retinoic Acid Inducible Gene 1 Protein Rabbit Anti-Human/Mouse SARM (Toll-Like Receptor Adaptor Protein, CT) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB Rabbit Anti-Human/Mouse SIGIRR (TLR Inhibitor, CT) 100 µg WB, IHC PK-AB ST2 (NT) antibody Rabbit Anti-Mouse ST2 (TLR Inhibitor, NT) 100 µg WB PK-AB TAB1 antibody Rabbit Anti-Human/Mouse TAB1 (TAK1 Binding Protein) 100 µg WB PK-AB TIRAP (CT) antibody TIRAP / MAL (IN) antibody TIRP (C2) antibody TIRP (CT) antibody Rabbit Anti-Human/Mouse/Rat TIRAP (Toll-Like Receptor Adaptor Protein, CT) Rabbit Anti-Human TIRAP (MAL, Toll-Like Receptor Adaptor Protein, IN) Rabbit Anti-Human/Mouse/Rat Toll/Interleukin-1 Receptor (Toll-Like Receptor Adaptor Protein, C2) Rabbit Anti-Human Toll/Interleukin-1 Receptor (Toll-Like Receptor Adaptor Protein, CT) 100 µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB TLR1 (IN) antibody Rabbit Anti-Human Toll-Like Receptor 1 (IN) 100 µg WB PK-AB TLR1 (NT) antibody Rabbit Anti-Human/Mouse Toll-Like Receptor 1 (NT) 100 µg WB PK-AB TLR2 (NT) antibody Rabbit Anti-Human/Mouse Toll-Like Receptor 2 (NT) 100 µg WB, IHC PK-AB TLR3 (CT) antibody Rabbit Anti-Human/Mouse Toll-Like Receptor 3 (CT) 100 µg WB PK-AB TLR3 (NT) antibody Rabbit Anti-Human/Mouse Toll-Like Receptor 3 (NT) 100 µg WB PK-AB TLR4 (NT) antibody Rabbit Anti-Human/Mouse/Rat Toll-Like Receptor 4 (NT) 100 µg WB PK-AB TLR4 (NT) antibody Rabbit Anti-Human Toll-Like Receptor 4 (NT) 100 µg WB, IHC PK-AB TLR5 (CT) antibody Rabbit Anti-Human/Mouse Toll-Like Receptor 5 (CT) 100 µg WB PK-AB TLR5 (IN) antibody Rabbit Anti-Human/Mouse/Rat Toll-Like Receptor 5 (IN) 100 µg WB PK-AB TLR6 (IN) antibody Rabbit Anti-Human Toll-Like Receptor 6 (IN) 100 µg WB PK-AB TLR6 (NT) antibody Rabbit Anti-Human/Mouse Toll-Like Receptor 6 (NT) 100 µg WB PK-AB TLR7 (IN) antibody Rabbit Anti-Human/Mouse Toll-Like Receptor 7 (IN) 100 µg WB PK-AB TLR8 (IN) antibody Rabbit Anti-Human/Mouse Toll-Like Receptor 8 (IN) 100 µg WB, IHC PK-AB TLR9 (CT) antibody Rabbit Anti-Human/Mouse Toll-Like Receptor 9 (CT) 100 µg WB PK-AB TLR9 (IN) antibody Rabbit Anti-Human/Mouse/Rat Toll-Like Receptor 9 (IN) 100 µg WB PK-AB TLR10 (IN) antibody Rabbit Anti-Human Toll-Like Receptor 10 (IN) 100 µg WB PK-AB TLR11 (CT) antibody Rabbit Anti-Mouse Toll-Like Receptor 11 (CT) 100 µg WB, IHC PK-AB TLR11 (IN) antibody Rabbit Anti-Mouse Toll-Like Receptor 11 (IN) 100 µg WB, IHC PK-AB TOLLIP (CT) antibody Rabbit Anti-Human/Mouse/Rat Toll Interacting Protein (CT) 100 µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

143 141 Product Name Product Description Size* Application** Catalog Number TOLLIP (IN) antibody Rabbit Anti-Human/Mouse/Rat Toll Interacting Protein (IN) 100 µg WB PK-AB TRIAD3A antibody Rabbit Anti-Mouse TRIAD3A (TLR Ubiquitinating Enzyme) 100 µg WB PK-AB TRIF / TICAM-1 antibody UBC13 antibody UEV1A antibody Rabbit Anti-Human TRIF (TICAM-1, Toll-Like Receptor Adaptor Protein) Rabbit Anti-Human/Mouse/Rat Ubiquitin Conjugating Enzyme 13 Rabbit Anti-Human/Mouse/Rat Ubiquitin Conjugating Enzyme 100 µg WB, IHC PK-AB µg WB PK-AB µg WB, IHC PK-AB n Homeostasis Product Name Product Description Size* Application** Catalog Number ATM antibody Rabbit Anti-Human ATM (Mutated in Ataxia-Telangiectasia) 100 µg WB PK-AB beta-actin antibody (mab) beta-actin antibody Mouse Anti-Human/Mouse/Rat/Pig/Sheep/Chicken/ Rabbit/Bovine beta-actin Rabbit Anti-Human/Mouse/Rat/Monkey/Hamster beta-actin 100 µg WB, IF, IHC PK-AB µg WB, IP, IHC PK-AB beta-actin antibody Rabbit Anti-Human/Mouse beta-actin 100 µg WB PK-AB beta-actin (CT) antibody beta-actin (NT) antibody BRCC36 antibody BRCC45 antibody Bub1 (CT) antibody Bub1 (NT) antibody Bub3 (CT) antibody CCDC98 (NT) antibody CLIP170 antibody CSN8 (CT) antibody EVER1 (IN) antibody EVER1 (NT) antibody EVER2 (IN) antibody Rabbit Anti-Human/Mouse/Rat beta-actin (Major Cytoskeletal Protein, CT) Rabbit Anti-Human/Mouse/Rat beta-actin (Major Cytoskeletal Protein, NT) Rabbit Anti-Human/Mouse/Rat BRCA1/BRCA2- containing Complex Subunit 36 Rabbit Anti-Human/Mouse/Rat BRCA1/BRCA2- containing Complex Subunit 45 Rabbit Anti-Human/Rat Bub1 (Kinase Involved in Spindle Formation, CT) Rabbit Anti-Human/Mouse Bub1 (Kinase Involved in Spindle Formation, NT) Rabbit Anti-Human/Mouse Bub3 (Kinase Involved in Spindle Formation, CT) Rabbit Anti-Human/Mouse CCDC98 (Coiled-coil Domain 98, NT) Rabbit Anti-Human/Mouse/Rat CLIP170 (CYLN1, Restin, RSN) Rabbit Anti-Human/Mouse/Rat CSN8 (COP9 Signalosome Complex Subunit 8) Rabbit Anti-Human/Mouse EVER1 (Transmembrane Protein Channel-like Protein, IN) Rabbit Anti-Human/Mouse EVER1 (Transmembrane Protein Channel-like Protein 6, NT) Rabbit Anti-Human/Mouse/Rat EVER2 (Transmembrane channel-like protein 8, TMC8, IN) 100 µg WB, ICC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, ICC PK-AB µg WB, ICC PK-AB µg WB, ICC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

144 142 Product Name Product Description Size* Application** Catalog Number EVER2 (NT) antibody Rabbit Anti-Human/Mouse/Rat EVER2 (Transmembrane channel-like protein 8, TMC8, NT) 100 µg WB PK-AB FABP7 (IN) antibody Rabbit Anti-Human Fatty Acid Binding Protein 7 (IN) 100 µg WB, IHC PK-AB GRTP1 (NT) antibody GSTP1 antibody LIMP2 antibody LIMP2 antibody LSD1 / AOF2 (IN) antibody LSD1 / AOF2 (NT) antibody MORF4 antibody Nephrin antibody op18 (CT) antibody op18 (NT) antibody Plxdc2 (CT) antibody Plxdc2 (NT) antibody PTK7 (CT) antibody PTK7 (NT) antibody RAP80 (CT) antibody RAP80 (NT) antibody RGP1 (NT) antibody RGPD5 (NT) antibody RPA Interacting Protein (CT) antibody RPA Interacting Protein (NT) antibody RTP801 (IN) antibody RTP801 (NT) antibody SCO1 antibody Rabbit Anti-Human/Mouse/Rat GRTP1 (Growth Hormone Regulated TBC Protein 1, NT) Rabbit Anti-Human/Mouse/Rat Glutathione S-Transferase pi Rabbit Anti-Human/Mouse/Rat LIMP2 (Lysosomal Integral Membrane Protein 2, CD36L2) Rabbit Anti-Human/Mouse LIMP2 (Lysosomal Integral Membrane Protein 2) Rabbit Anti-Human/Mouse/Rat Lysine Specific Histone Demethylase 1 (AOF2, IN) Rabbit Anti-Human/Mouse/Rat Lysine Specific Histone Demethylase 1 (AOF2, NT) Rabbit Anti-Human/Mouse/Rat MORF4 (Senescence-related Protein) Rabbit Anti-Human/Mouse/Rat Nephrin (Kidney Glomerulus-specific Cell Adhesion Receptor) Rabbit Anti-Human/Mouse op18 (Member of the Stahmin Family of Proteins, CT) Rabbit Anti-Human/Mouse/Rat op18 (Member of the Stahmin Family of Proteins, NT) Rabbit Anti-Human/Mouse/Rat Plxdc2 (TEM7 Receptor, CT) Rabbit Anti-Human/Mouse/Rat Plxdc2 (TEM7 Receptor, NT) Rabbit Anti-Human/Mouse/Rat Protein Tyrosine Kinase 7 (CT) Rabbit Anti-Human/Mouse/Rat Protein Tyrosine Kinase 7 (NT) Rabbit Anti-Human/Mouse Receptor-associated Protein 80 (CT) Rabbit Anti-Human/Mouse Receptor-associated Protein 80 (NT) Rabbit Anti-Human/Mouse/Rat RGP1 (Retrograde Golgi Transport Homolog 1, NT) Rabbit Anti-Human/Mouse/Rat RGPD5 (RANBP2-like & GRIP Domain-containing Protein 5, RGP5, NT) Rabbit Anti-Human/Mouse/Rat RPA Interacting Protein (CT) Rabbit Anti-Human/Mouse/Rat RPA Interacting Protein (NT) Rabbit Anti-Human/Mouse/Rat RTP801 (DNA Damage-inducible Transcript 4, IN) Rabbit Anti-Human/Mouse/Rat RTP801 (DNA Damage-inducible Transcript 4, NT) Rabbit Anti-Human/Mouse/Rat SCO1 (Synthesis of Cytochrome c Oxidase 2) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

145 143 Product Name Product Description Size* Application** Catalog Number SCO2 antibody Rabbit Anti-Human SCO2 (Synthesis of Cytochrome c Oxidase 2) 100 µg WB, IHC PK-AB SIRT2 (CT) antibody Rabbit Anti-Human/Mouse/Rat Sirtuin 2 (CT) 100 µg WB PK-AB SIRT2 (NT) antibody Rabbit Anti-Human/Mouse/Rat Sirtuin 2 (NT) 100 µg WB PK-AB Slc22A17 antibody Slc35D1 (CT) antibody Slc35D1 (NT) antibody STEAP1 (CT) antibody STEAP1 (IN) antibody STEAP2 antibody STEAP3 antibody STEAP4 antibody TBC1D1 (CT) antibody TEM1 (CT) antibody TEM1 (NT) antibody TEM2 (CT) antibody TEM2 (NT) antibody Rabbit Anti-Human/Mouse/Rat Slc22A17 (Solute Carrier Family 22 Member 17) Rabbit Anti-Human Slc35D1 (Solute Carrier Family 35 Member D1) Rabbit Anti-Human/Mouse/Rat Slc35D1 (Solute Carrier Family 35 Member D1) Rabbit Anti-Human/Mouse/Rat Six Transmembrane Epithelial Antigen of Prostate 1 (CT) Rabbit Anti-Human/Mouse/Rat Six Transmembrane Epithelial Antigen of Prostate 1 (IN) Rabbit Anti-Human/Mouse Six Transmembrane Epithelial Antigen of Prostate 2 Rabbit Anti-Human/Mouse/Rat Six Transmembrane Epithelial Antigen of Prostate 3 Rabbit Anti-Human/Mouse/Rat Six Transmembrane Epithelial Antigen of Prostate µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB Rabbit Anti-Human TBC1D1 (Rab GAP, CT) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Tumor Endothelial Marker 1 (CT) Rabbit Anti-Human/Mouse/Rat Tumor Endothelial Marker 1 (NT) Rabbit Anti-Human/Mouse/Rat Tumor Endothelial Marker 2 (CT) Rabbit Anti-Human/Mouse/Rat Tumor Endothelial Marker 2 (NT) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB TEM4 (IN) antibody Rabbit Anti-Human Tumor Endothelial Marker 4 (IN) 100 µg WB, IHC PK-AB TEM5 (CT) antibody Rabbit Anti-Human/Rat Tumor Endothelial Marker 5 (CT) 100 µg WB, IHC PK-AB TEM5 (IN) antibody Rabbit Anti-Human/Rat Tumor Endothelial Marker 5 (IN) 100 µg WB, IHC PK-AB TEM7 (CT) antibody TEM7 (IN) antibody TNPO3 (NT) antibody TNPO3 (NT2) antibody TP1 antibody TRIM25 (CT) antibody TRIM25 (NT) antibody Rabbit Anti-Human/Mouse/Rat Tumor Epithelial Marker 7 (CT) Rabbit Anti-Human/Mouse/Rat Tumor Epithelial Marker 7 (IN) Rabbit Anti-Human/Mouse/Rat TNPO3 (Transportin-3, Importin-12, NT) Rabbit Anti-Human/Mouse/Rat TNPO3 (Transportin-3, Importin-12, NT2) Rabbit Anti-Human/Mouse/Rat Telomerase-associated Protein 1 Rabbit Anti-Human/Mouse TRIM25 (Estrogen-responsive Finger Protein, CT) Rabbit Anti-Human TRIM25 (Estrogen-responsive Finger Protein, NT) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB, ICC PK-AB µg WB, ICC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

146 144 Product Name Product Description Size* Application** Catalog Number TRT antibody Rabbit Anti-Human Telomerase Reverse Transcriptase 100 µg WB, IHC PK-AB Unc93a antibody Rabbit Anti-Human Unc93a 100 µg WB PK-AB VPS53 (CT) antibody VPS53 (IN) antibody Rabbit Anti-Human/Mouse/Rat VPS53 (Vacuolar Protein Sorting 53, HCCS1, CT) Rabbit Anti-Human/Mouse/Rat VPS53 (Vacuolar Protein Sorting 53, IN) 100 µg WB PK-AB µg WB PK-AB n Diseases & Oncology Product Name Product Description Size* Application** Catalog Number Cathepsin B antibody Cathepsin D antibody Cathepsin F antibody Cathepsin G antibody Cathepsin H antibody Cathepsin K antibody Rabbit Anti-Human Cathepsin B 100 µg WB, IHC PK-AB Rabbit Anti-Human Cathepsin D 100 µg WB, IHC PK-AB Rabbit Anti-Human/Mouse Cathepsin F 100 µl WB PK-AB Rabbit Anti-Human Cathepsin G 200 µl WB PK-AB Goat Anti-Human/Mouse/Rat Cathepsin H 100 µg WB PK-AB G Rabbit Anti-Human/Mouse/Rat Cathepsin K 100 µg WB, IP, IHC PK-AB Cathepsin K antibody Cathepsin L (cleaved) antibody Cathepsin L antibody (mab) Cathepsin L antibody (mab) Cathepsin L antibody (mab), biotin Cathepsin L antibody (mab), FITC Cathepsin L antibody Cathepsin P antibody Cathepsin S antibody Cathepsin S antibody Cathepsin V antibody (mab) Cathepsin V antibody Rabbit Anti-Human/Mouse/Rat/Chicken/Pig/Rabbit Cathepsin K 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Cathepsin L 100 µg WB PK-AB Mouse Anti-Human Cathepsin L (clone CLP 1/36) 100 µg WB, IHC PK-AB Mouse Anti-Human Cathepsin L (clone 33-1) 100 µg E, WB PK-AB Mouse Anti-Human Cathepsin L (clone 33-1; biotinylated) Mouse Anti-Human Cathepsin L (clone 33-1; FITC-labeled) 100 tests 100 tests not tested not tested PK-AB B PK-AB F Rabbit Anti-Human Cathepsin L 100 µg WB PK-AB Rabbit Anti-Human Cathepsin P 200 µl WB PK-AB Rabbit Anti-Human Cathepsin S 100 µg WB PK-AB Goat Anti-Human/Mouse/Rat Cathepsin S 100 µg WB, IP, IHC PK-AB G Mouse Anti-Human Cathepsin V (clone 55-1C5) 100 µg WB, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Cathepsin V 100 µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

147 145 Product Name Product Description Size* Application** Catalog Number Cathepsin W antibody (mab) MnSOD antibody (mab) MnSOD antibody (mab), FITC p185(her-2) antibody (mab) p185(her-2) antibody (mab), biotin p185(her-2) antibody (mab), FITC Podoplanin antibody (mab) Podoplanin antibody (mab) Mouse Anti-Human Cathepsin W (clone 39-2B6) 100 µg WB, IHC PK-AB Mouse Anti-Human Manganese Superoxide Dismutase (clone MnS-1) Mouse Anti-Human Manganese Superoxide Dismutase (clone MnS-1; FITC-labeled) Mouse Anti-Human p185 (Human EGF Receptor 2; clone 2G11) Mouse Anti-Human p185 (Human EGF Receptor 2; clone 2G11; biotinylated) Mouse Anti-Human p185 (Human EGF Receptor 2; clone 2G11; FITC-labeled) 100 µg IHC, WB PK-AB tests not tested PK-AB F 100 µg E, WB PK-AB tests 100 tests not tested not tested Mouse Anti-Human Podoplanin 100 µg WB, IHC, FC, IF Hamster Anti-Mouse Podoplanin 100 µg WB, IHC, FC (IS) PK-AB B PK-AB F PK-AB PK-AB HM n Phospho-specific Antibodies Product Name Product Description Size* Application** Catalog Number Phospho-AFX antibody Rabbit Anti-Human/Mouse Phospho-AFX 100 µg WB PK-AB Phospho-Akt antibody Rabbit Anti-Human/Mouse/Rat Phospho-Akt 100 µg WB, IP PK-AB Phospho-ATF-2 antibody Rabbit Anti-Human/Mouse/Rat Phospho-ATF µg WB, IP, IHC PK-AB Phospho-Bad antibody Rabbit Anti-Human/Mouse/Rat Phospho-Bad 100 µg WB, IP, IHC PK-AB Phospho-b-Catenin antibody Phospho-BRCA1 (Ser1189) antibody Phospho-CaMKII P antibody Rabbit Anti-Human/Mouse/Rat Phospho-beta- Catenin 100 µg WB PK-AB Rabbit Anti-Human Phospho-BRCA1 (Ser1189) 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Phospho-CaMKII P 100 µg WB PK-AB Phospho-c-Jun antibody Rabbit Anti-Human/Mouse/Rat Phospho-c-Jun 100 µg WB, IHC PK-AB Phospho-c-Jun (Ser73) antibody Phospho-c-Myc antibody Phospho-DARPP-32 antibody Rabbit Anti-Human/Mouse/Rat Phospho-c-Jun (Ser73) 100 µg WB, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Phospho-c-Myc 100 µg WB, IP, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Phospho-DARPP µl WB PK-AB Phospho-Elk-1 antibody Rabbit Anti-Human/Mouse/Rat Phospho-Elk µg WB PK-AB Phospho-Erk1/2 antibody Rabbit Anti-Human/Mouse/Rat Phospho-Erk1/2 (p44/42 MAPK) 100 µg WB, IP, IHC PK-AB Phospho-FAK antibody Rabbit Anti-Human/Mouse/Rat Phospho-FAK 100 µg WB, IF PK-AB Phospho-GSK-3a antibody Rabbit Anti-Human/Mouse/Rat Phospho-GSK-3 alpha 100 µg WB, IHC PK-AB Phospho-GSK-3b antibody Rabbit Anti-Human/Mouse/Rat Phospho-GSK-3 beta 100 µg WB, IHC PK-AB Phospho-JKK/SEK1/ MKK4 antibody Rabbit Anti-Human/Mouse/Rat Phospho-JKK (SEK1/ MKK4) 100 µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

148 146 Product Name Product Description Size* Application** Catalog Number Phospho-JNK/SAPK (Thr183/Tyr185) antibody Rabbit Anti-Human/Mouse/Rat Phospho-JNK/SAPK (Thr183/Tyr185) 100 µg WB, IP, IHC PK-AB Phospho-Lck antibody Rabbit Anti-Human Phospho-Lck 100 µg WB, IP, IHC PK-AB Phospho-MAPKAPK-2 antibody Phospho-MARCKS antibody Rabbit Anti-Human/Mouse Phospho-MAPKAPK µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Phospho-MARCKS 100 µl WB PK-AB Phospho-Mek1/2 antibody Rabbit Anti-Human/Mouse/Rat Phospho-Mek1/2 100 µl WB, IF PK-AB Phospho-MLK3 antibody Phospho-MSK1 antibody Phospho-p38 MAPK antibody Phospho-p53 (Ser15) antibody Phospho-p70 S6 Kinase (Thr389) antibody Rabbit Anti-Human/Mouse Phospho-MLK3 100 µg WB PK-AB Rabbit Anti-Human/Mouse Phospho-MSK1 100 µg WB PK-AB Rabbit Anti-Human/Mouse/Rat Phospho-p38 MAPK 100 µg WB, IP, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Phospho-p53 (Ser15) 100 µg WB, IP, IHC PK-AB Rabbit Anti-Human/Mouse/Rat Phospho-p70 S6 Kinase (Thr389) 100 µg WB PK-AB Phospho-PKC antibody Rabbit Anti-Human/Mouse/Rat Phospho-PKC 100 µg WB PK-AB Phospho-Raf (Ser259) antibody Rabbit Anti-Human/Mouse/Rat Phospho-Raf (Ser259) 100 µg WB, IHC PK-AB Phospho-Stat1 antibody Rabbit Anti-Human/Mouse/Rat Phospho-Stat1 100 µg WB, IF PK-AB Phospho-Stat2 antibody Rabbit Anti-Human/Mouse Phospho-Stat2 100 µg WB PK-AB Phospho-Stat3 antibody Rabbit Anti-Human/Mouse Phospho-Stat3 100 µg WB, IP, IHC PK-AB Phospho-Stat5 antibody Rabbit Anti-Human/Mouse/Rat Phospho-Stat5 100 µg WB, IP, IHC PK-AB Phospho-Stat6 antibody Rabbit Anti-Human/Mouse Phospho-Stat6 100 µg WB PK-AB Phospho-Tau antibody Goat Anti-Human/Mouse/Rat Phospho-Tau 100 µg WB PK-AB G Phospho-TrkA (Tyr490) antibody Phospho-Tyrosine Hydroxylase antibody Rabbit Anti-Human/Mouse/Rat Phospho-TrkA (Tyr490) Rabbit Anti-Human/Mouse/Rat Phospho-Tyrosine Hydroxylase 100 µg WB, IP, IF PK-AB µl WB, IF, IHC PK-AB Phospho-VASP antibody Rabbit Anti-Human/Mouse/Rat Phospho-VASP 100 µg WB PK-AB n Immunology Product Name Product Description Size* Application** Catalog Number ADAP (CT) antibody ADAP (NT) antibody Blimp-1 (CT) antibody Blimp-1 (NT) antibody Rabbit Anti-Human/Mouse Adhesion and Degranulation Adapter Protein (CT) Rabbit Anti-Human/Mouse Adhesion and Degranulation Adapter Protein (NT) Rabbit Anti-Human/Mouse/Rat B-Lymphocyte Induced Maturation Protein 1 (CT) Rabbit Anti-Human/Mouse/Rat B-Lymphocyte Induced Maturation Protein 1 (NT) 100 µg WB, ICC PK-AB µg WB, ICC PK-AB µg WB, IF PK-AB µg WB, IHC PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

149 147 Product Name Product Description Size* Application** Catalog Number Carabin (CT) antibody Carabin (NT) antibody MICA antibody ORAI1 (CT) antibody ORAI1 (NT) antibody ORAI2 (CT) antibody ORAI3 (CT) antibody ORAI3 (NT) antibody Rabbit Anti-Human/Mouse Carabin (Negative Regulator of the TCR Signaling Pathway, CT) Rabbit Anti-Human/Mouse Carabin (Negative Regulator of the TCR Signaling Pathway, NT) Rabbit Anti-Human/Mouse MHC Class I Chain-related Protein A Rabbit Anti-Human/Mouse/Rat ORAI1 (T-cell Calcium Channel, CT) Rabbit Anti-Human/Mouse ORAI1 (T-cell Calcium Channel, NT) Rabbit Anti-Human/Mouse ORAI2 (T-cell Calcium Channel, CT) Rabbit Anti-Human/Mouse ORAI3 (T-cell Calcium Channel, CT) Rabbit Anti-Human/Mouse/Rat ORAI3 (T-cell Calcium Channel, NT) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, ICC PK-AB µg WB, ICC PK-AB µg WB, ICC PK-AB n Innate Immunity Product Name Product Description Size* Application** Catalog Number Cathelicidin antibody IPR1 (CT) antibody IPR1 (NT) antibody Rabbit Anti-Human/Mouse/Rat Cathelicidin (CAP18, FALL39, LL37) Rabbit Anti-Human/Mouse/Rat Intracellular Pathogen Resistance Protein 1 (CT) Rabbit Anti-Human/Mouse/Rat Intracellular Pathogen Resistance Protein 1 (NT) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB LGP2 (CT) antibody Rabbit Anti-Human/Mouse/Rat LGP2 (DHX58, CT) 100 µg WB PK-AB LGP2 (IN) antibody Rabbit Anti-Human/Mouse/Rat LGP2 (DHX58, IN) 100 µg WB PK-AB LGP2 (NT) antibody Rabbit Anti-Human/Mouse/Rat LGP2 (DHX58, NT) 100 µg WB PK-AB MDA5 (CT) antibody MDA5 (IN) antibody SLPI antibody SLPI antibody Rabbit Anti-Human/Mouse Melanoma Differentiation Associated Protein 5 (CT) Rabbit Anti-Human/Mouse Melanoma Differentiation Associated Protein 5 (IN) Rabbit Anti-Human/Mouse Secretory Leukocyte Protease Inhibitor Rabbit Anti-Mouse Secretory Leukocyte Protease Inhibitor 100 µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB, IHC PK-AB µg WB PK-AB Unc93b antibody Rabbit Anti-Human/Mouse/Rat Unc93b 100 µg WB PK-AB VISA (IN) antibody VISA (NT) antibody Rabbit Anti-Human/Mouse/Rat Virus Induced Signaling Adapter (IN) Rabbit Anti-Human/Mouse/Rat Virus Induced Signaling Adapter (NT) 100 µg WB, IHC PK-AB µg WB, IHC PK-AB ZBP1 antibody Rabbit Anti-Human/Mouse/Rat Z-DNA Binding Protein µg WB PK-AB PAP-1 antibody Rabbit Anti-Human Pancreatitis Associated Protein 100 µg E PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

150 148 n Autophagy Product Name Product Description Size* Application** Catalog Number Ambra1 antibody (CT) Ambra1 antibody (NT) ATG5 antibody Rabbit Anti-Human/Mouse/Rat Ambra1 (Activating Molecule in Beclin-1 Regulated Autophagy, WDR94) Rabbit Anti-Human/Mouse Ambra1 (Activating Molecule in Beclin-1 Regulated Autophagy, WDR94) Rabbit Anti-Human/Mouse/Rat Autophagy-related Protein µg WB PK-AB µg WB PK-AB µg WB PK-AB ATG10 antibody Rabbit Anti-Human/Mouse/Rat Autophagy Protein µg WB PK-AB ATG12 (IN) antibody ATG12 (NT) antibody ATG16 (IN) antibody ATG16 (NT) antibody IRGM (IN) antibody IRGM (NT) antibody Rabbit Anti-Human/Mouse/Rat Autophagy-related Protein 12 (IN) Rabbit Anti-Human/Mouse/Rat Autophagy-related Protein 12 (NT) Rabbit Anti-Human/Mouse/Rat Autophagy-related Protein 16 (IN) Rabbit Anti-Human/Mouse/Rat Autophagy-related Protein 16 (NT) Rabbit Anti-Human/Mouse/Rat IRGM (Immunity Related GTPase Family, IFL1, LRG47, IN) Rabbit Anti-Human/Mouse/Rat IRGM (Immunity Related GTPase Family, IFL1, LRG47, NT) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB n Anti-Tag Antibodies Product Name Product Description Size* Application** Catalog Number FLAG-Tag antibody (mab) Mouse Anti-FLAG Peptide (clone PFLAGSHG) 100 µg WB PK-AB FLAG-Tag antibody (mab) Mouse Anti-FLAG Peptide 500 µg WB, E, IHC PK-AB FLAG-HRP antibody (mab) GFP antibody (mab) GFP antibody Mouse Anti-FLAG Peptide (peroxidase-conjugated; clone PFLAGHRPSHG) Mouse Anti-Green Fluorescent Protein (wild-type & derivatives) Rabbit Anti Green Fluorescent Protein (wild-type & derivatives) 100 µl WB PK-AB µg WB, IP, IHC PK-AB µg WB, IP PK-AB GST-Tag antibody (mab) Mouse Anti-Glutathione-S-transferase (clone PGSTSHG) 100 µg WB, IP PK-AB GST-HRP antibody (mab) Mouse Anti-Glutathione-S-transferase (peroxidaseconjugated; clone PGSTHRPSHG) 100 µl WB PK-AB GST-Tag antibody Rabbit Anti-Glutathione-S-transferase 100 µg WB PK-AB HA-Tag antibody (mab) Mouse Anti-Hemagglutinin Tag (clone PHASHG) 100 µg WB PK-AB HA-Tag antibody (mab) Mouse Anti-Hemagglutinin Tag (clone 12CA5) 200 µg WB, IP, IF PK-AB HA-Biotin antibody (mab) Mouse Anti-Hemagglutinin Tag/Biotin (clone 12CA5) 100 µg WB, IP, IF PK-AB B HA-FITC antibody (mab) Mouse Anti-Hemagglutinin Tag/FITC (clone 12CA5) 100 µg WB, IP, IF PK-AB F HA-Biotin antibody (mab) Mouse Anti-Hemagglutinin Tag/Biotin (clone 2C16) 100 µg WB, IP, IF PK-AB B HA-HRP antibody (mab) Mouse Anti-Hemagglutinin Tag (peroxidase-conjugated; clone PHAHRPSHG) 100 µl WB PK-AB His-Tag antibody (mab) Mouse Anti-Polyhistidine Tag (clone PHISSHG) 100 µg WB, IP PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

151 149 Product Name Product Description Size* Application** Catalog Number His-Tag antibody Rabbit Anti-Polyhistidine Tag 100 µg WB, IP, IF PK-AB MBP antibody (mab) Mouse Anti-Maltose Binding Protein 20 µg WB, IF PK-AB Myc-HRP antibody (mab) Mouse Anti-Myc (peroxidase-conjugated; clone PMYCHRPSHG) 100 µl WB PK-AB Myc-Tag antibody (mab) Mouse Anti Human c-myc (clone NYRhc-myc) 500 µg WB PK-AB Myc-Tag antibody (mab) Mouse-Anti-Myc (clone PMYCSHG) 100 µg WB, IP PK-AB Myc-Tag antibody Rabbit Anti-Myc 100 µg WB, IP, IF PK-AB RFP antibody (mab) Mouse Anti-Red Fluorescent Protein (ds-red) 100 µg WB, IP, IHC PK-AB RFP antibody Rabbit Anti-Red Fluorescent Protein 100 µg WB PK-AB n Infectious Diseases Product Name Product Description Size* Application** Catalog Number Chlamydia LPS antibody (mab) Mouse Anti-Chlamydia LPS 500 µg E PK-AB NG antibody (mab) Mouse Anti-Neisseria Gonorrhea 500 µg E PK-AB n Cell Signaling & Cell Stress Product Name Product Description Size* Application** Catalog Number HEP antibody (mab) HEP-1 / HPA-1 antibody Mouse Anti-Human Heparanase-1 (clone HP130) 100 µg WB, IHC, IP, FC, IF PK-AB Rabbit Anti-Human Heparanase µg WB, IHC PK-AB n Obesity Product Name Product Description Size* Application** Catalog Number ELOVL6 antibody GPAT1 antibody Lipe / HSL antibody UROP11 antibody Rabbit Anti-Human/Mouse/Rat ELOVL6 (Elongation of Very Long Chain Fatty Acids Protein 6, FACE, LCE) Rabbit Anti-Human/Mouse/Rat GPAT1 (Glycerol-3-phosphate Acyltransferase 1, GPAM) Rabbit Anti-Human/Mouse/Rat Lipe (Hormone Sensitive Lipase) Rabbit Anti-Human/Mouse/Rat UROP11 (Upregulated obese product 11) 100 µg WB PK-AB µg WB PK-AB µg WB PK-AB µg WB PK-AB Abbreviations: see page 150 Please note: For most antibodies we provide control lysates of cell lines & tissues as well as blocking peptides for testing. Most of the PK-AB815 antibodies are available as conjugates with biotin. Please request! * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

152 150 n Miscellaneous Antibodies Product Name Product Description Size* Application** Catalog Number Aminopeptidase P (epithelial) antibody (mab) DC Marker antibody (mab) EndoGlyx-1 antibody (mab) EpCAM-1 antibody (mab) Mouse Anti-Rat Epithelial Aminopeptidase P (clone JG12C9C10) 100 µg IHC (P) PK-AB MR Rat Anti-Mouse Dendritic Cell Marker 200 µg IHC (P), FC PK-AB RM Mouse Anti-Human EndoGlyx 1 (clone H572) 100 µg FC, IHC, IP PK-AB Mouse Anti-Human EpCAM-1 (clone VU-1D9) 100 µg FC, FST (BL), IHC (P), IP, WB PK-AB Factor-IX antibody (mab) Mouse Anti-Factor IX (clone NYRhFIX) 500 µg WB, E, IHC PK-AB FAP antibody (mab) GH antibody (mab) Glutathione antibody (mab) hcg-beta Core antibody (mab) Mouse Anti-Human Fibroblast Activation Protein (clone F11-24) Mouse Anti-Human Growth Hormone (clone PG3H5AT) 100 µg FC, IHC, IP PK-AB µg WB, E PK-AB Mouse Anti-Glutathione-S-Transferase (GST) 200 µg NA PK-AB Mouse Anti-Human Chorionic Gonadotropin beta Core 500 µg E PK-AB HEL antibody (mab) Mouse Anti-Hen Egg Lysozyme 500 µg E PK-AB HEP-1 / HPA-1 antibody (mab) Mouse Anti-Human Heparanase 1 (3/17) 50 µg WB, IHC PK-AB IgA (1&2) antibody (mab) Mouse Anti-Human IgA (1&2) 500 µg E PK-AB IgA (sec. comp.) antibody (mab) Mouse Anti-Human IgA Secretory Component 500 µg E PK-AB IgG-Fc antibody (mab) Mouse Anti-Human IgG-Fc 500 µg E PK-AB IgK antibody (mab) Mouse Anti-Human Ig Kappa Light Chain 500 µg E PK-AB IgL antibody (mab) Mouse Anti-Human Ig Lambda Light Chain 500 µg E PK-AB LDH antibody Luciferase antibody Macrophages antibody (mab) MadCAM-1 antibody (mab) Megalin / LRP2 antibody (mab) Goat Anti-Human/Mouse/Rat/Rabbit Lactate Dehydrogenase 500 µg WB PK-AB G Goat Anti-Luciferase (Firefly) 500 µg E, WB, IHC PK-AB G Rat Anti-Mouse Macrophages 200 µg IHC, IP, FC PK-AB RM Mouse Anti-Human MadCAM-1 (clone CA102.2C1) 100 µg E, WB, FST (BL) PK-AB Mouse Anti-Human Megalin (LRP2; clone CD7D5) 100 µg IHC PK-AB MOG antibody (mab) Mouse Anti-Myelin Oligodendrocyte Glycoprotein 500 µg E, WB, IP, IHC PK-AB MPO antibody Rabbit Anti-Human/Mouse Myeloperoxidase 100 µg WB PK-AB Plectin antibody (mab) Mouse Anti-Human Plectin (clone 10F6) 50 µg IHC, WB PK-AB Plectin antibody (mab), biotin Mouse Anti-Human Plectin (clone 10F6; biotinylated) 100 tests not tested PLP antibody (mab) Mouse Anti-Porteolipid Protein of Myelin (clone NYRPLP) 500 µg WB, IHC, IP, E PK-AB B PK-AB Abbreviations: A: activating antibody, B: bovine, C: chicken, CS: cell staining, CT: C-terminus, E: ELISA, ED: extracellular domain, EL: extracellular loop, FACS/FC: fluorescence-activated cell sorting, FST (AI): functional studies (apoptosis induction), FST (BL): functional studies (blocking), Gt: goat, H: human, Hs: horse, Ht: hamster, ICC: immunocytochemistry, ID: intracellular domain, IF: immunofluorescence microscopy, IHC (P): immunohistochemistry (paraffin), IN: intermediate domain, IP: immunoprecipitation, IS: immunostaining, M: mouse, mab: monoclonal antibody, Mk: monkey, N: Neutralization, NT: N-terminus, P: pig, pab: polyclonal antibody, R: rat, Rb: rabbit, WB: Westem blot * Other sizes available on request! ** Applications tested so far - other applications may be also possible! *** Unpurified serum

153 Labeled Antibodies and Labeling Kits - n Fluorescently and HRP-Labeled Antibodies PromoKine is offering secondary antibodies which are fluorescently labeled or conjugated to horseradish peroxidase (HRP), alkaline phosphatase (AP), or biotin. All antibodies are purified and highly active, and they can be used for diverse applications including ELISAs, immunofluorescence microscopy, immunohistochemistry, and Western blotting. Product Name Product Description Size* Application Format Catalog Number Donkey Anti-Goat antibody, Fluorescein-coupled Donkey Anti-Goat IgG conjugated to Fluorescein (H&L) 2 mg WB, E, IHC PK-AB Donkey Anti-Goat antibody, Texas Red-coupled Donkey Anti-Goat IgG conjugated to Texas Red (H&L) 2 mg WB, E, IHC PK-AB Goat Anti-Chicken antibody, Texas Red-coupled Goat Anti-Chicken IgG conjugated to Texas Red (H&L) 2 mg WB, E, IHC PK-AB Goat Anti-Mouse antibody, AP-coupled Goat Anti-Mouse IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity) 1 mg WB, E, IHC PK-AB Goat Anti-Mouse antibody, Biotin-coupled Goat Anti-Mouse IgG conjugated to Biotin (H&L, minimum cross-reactivity) 2 mg E PK-AB Goat Anti-Mouse antibody, Fluorescein-coupled Goat Anti-Mouse IgG conjugated to Fluorescein (H&L, minimum cross-reactivity) 2 mg IF, FC PK-AB Goat Anti-Mouse antibody, HRP-coupled Goat Anti-Mouse IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity) 2 mg WB, E, IHC PK-AB Goat Anti-Mouse antibody, Texas Red-coupled Goat Anti-Mouse IgG conjugated to Texas Red (H&L) 2 mg WB, E, IHC PK-AB Goat Anti-Rabbit antibody, AP-coupled Goat Anti-Rabbit IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity) 1 mg WB, E, IHC PK-AB Goat Anti-Rabbit antibody, Biotin-coupled Goat Anti-Rabbit IgG conjugated to Biotin (H&L, minimum cross-reactivity) 2 mg E PK-AB Goat Anti-Rabbit antibody, Fluorescein-coupled Goat Anti-Rabbit IgG conjugated to Fluorescein (H&L, minimum cross-reactivity) 2 mg IF, FC PK-AB Goat Anti-Rabbit antibody, HRP-coupled Goat Anti-Rabbit IgG Conjugated to Horseradish Peroxidase 0.5 ml WB, E, ICH P PK-AB Goat Anti-Rabbit antibody, HRP-coupled Goat Anti-Rabbit antibody, Texas Red-coupled Goat Anti-Rabbit IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity) Goat Anti-Rabbit IgG conjugated to Texas Red (H&L) 2 mg WB, E, IHC PK-AB mg WB, E, IHC PK-AB Goat Anti-Rat antibody, AP-coupled Goat Anti-Rat IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity) 1 mg WB, E, IHC PK-AB Goat Anti-Rat antibody, HRP-coupled Goat Anti-Rat IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity) 2 mg WB, E, IHC PK-AB Goat PF-XXX Anti-Human antibody PromoFluor-Labeled Goat Anti-Human IgG 1 mg WB, IF, E, IHC P PK-PF-xxx-AK-H1 Goat PF-XXX Anti-Mouse antibody PromoFluor-Labeled Goat Anti-mouse IgG 1 mg WB, IF, E, IHC P PK-PF-xxx-AK-M1 Goat PF-XXX Anti-Rabbit antibody PromoFluor-Labeled Goat Anti-Rabbit IgG 1 mg WB, IF, E, IHC P PK-PF-xxx-AK-R1 Abbreviations: see page 153 * Other sizes available on request ** not available in Japan, China & Korea PF-xxx- stands for the respective PromoFluor dye (PF-405, PF-415, PF-488, PF-488LSS, PF-514LSS, PF-546, PF-555, PF-590, PF-633, PF-647, PF-670, PF- 700, PF-750, PF-770; Protein Labling Kits with other PromoFluor dyes are available on request!)

154 152 - Product Name Product Description Size* Application Format Catalog Number Rabbit Anti-Chicken antibody, AP-coupled Rabbit Anti-Chicken antibody, HRP-coupled Rabbit Anti-Goat antibody, AP-coupled Rabbit Anti-Goat antibody, HRP-coupled Rabbit Anti-Goat antibody, HRP-coupled Rabbit Anti-Mouse antibody, HRP-coupled Rabbit Anti-Chicken IgG conjugated to Alkaline Phosphatase (H&L) Rabbit Anti-Chicken IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity) Rabbit Anti-Goat IgG conjugated to Alkaline Phosphatase (H&L, minimum cross-reactivity) Rabbit Anti-Goat IgG Conjugated to Horseradish Peroxidase Rabbit Anti-Goat IgG conjugated to Horseradish Peroxidase (H&L, minimum cross-reactivity) Rabbit Anti-Mouse IgG Conjugated to Horseradish Peroxidase 1 mg E PK-AB mg WB, E, IHC PK-AB mg WB, E, IHC PK-AB ml WB, E, ICH P PK-AB mg WB, E, IHC PK-AB ml WB, E, ICH P PK-AB Rabbit PF-XXX Anti- Fluorescein antibody PromoFluor-Labeled Rabbit Anti-Fluorescein IgG 1 mg WB, IF, E, IHC P PK-PF-xxx-AK-F1 n Protein and Antibody Labeling Kits PromoKine also offers ready-to-use Protein and Antibody Labeling Kits. These innovative kits have been developed for convenient and highly efficient labeling specific antibodies or target proteins. The kits include either superior Promo- Fluor dyes or other common fluorescent dyes and biomarkers such as fluorescein, rhodamine, sulforhodamine, R-phycoerythrin, allophycocyanin, biotin, Peroxidase, or Alkaline Phosphatase (AP). Product Name Product Description Size* Catalog Number PF-xxx-Labeling-Kit Kit for Labeling of Proteins/Antibodies with a Selected PromoFluor Dye 5 reactions PK-PF-LK-XXX-05 PF-xxx-Labeling-Kit Kit for Labeling of Proteins/Antibodies with a Selected PromoFluor Dye 10 reactions PK-PF-LK-XXX-10 Fluorescein Labeling Kit-NH2 Rhodamine 110 Labeling Kit-NH2 Rhodamine 110 Labeling Kit-SH Kit for Labeling of Proteins/Antibodies with Fluorescein Kit for Labeling of Proteins/Antibodies with Rhodamine 110 Kit for Labeling of Proteins/Antibodies with Rhodamine samples PK-PF-FLU-LK01** 3 samples PK-PF-RHO-LK27** 3 samples PK-PF-RHO-LK28** Sulforhodamine 101 Labeling Kit-NH2 Kit for Labeling of Proteins/Antibodies with Sulforhodamine samples PK-PF-SRH-LK29** Peroxidase Labeling Kit-NH2 Kit for Labeling of Proteins/Antibodies with Peroxidase 3 samples PK-CA-POX-LK11** Peroxidase Labeling Kit-SH Kit for Labeling of Proteins/Antibodies with Peroxidase 3 samples PK-CA-POX-LK09** Alkaline Phosphatase Labeling Kit-NH2 Alkaline Phosphatase Labeling Kit-SH Kit for Labeling of Proteins/Antibodies with Alkaline Phosphatase (AP) Kit for Labeling of Proteins/Antibodies with Alkaline Phosphatase (AP) 3 samples PK-CA-APH-LK12** 3 samples PK-CA-APH-LK13** Abbreviations: see page 153 * Other sizes available on request ** not available in Japan, China & Korea PF-xxx- stands for the respective PromoFluor dye (PF-405, PF-415, PF-488, PF-488LSS, PF-514LSS, PF-546, PF-555, PF-590, PF-633, PF-647, PF-670, PF- 700, PF-750, PF-770; Protein Labling Kits with other PromoFluor dyes are available on request!)

155 153 - Product Name Product Description Size* Catalog Number Biotin Labeling Kit-NH 2 Kit for Labeling of Proteins/Antibodies with Biotin 3 samples PK-CA-BIO-LK03** Biotin Labeling Kit-SH Kit for Labeling of Proteins/Antibodies with Biotin 3 samples PK-CA-BIO-LK10** B-Phycoerythrin Labeling Kit-NH 2 B-Phycoerythrin Labeling Kit-SH R-Phycoerythrin Labeling Kit-NH 2 R-Phycoerythrin Labeling Kit-SH Allophycocyanin Labeling Kit-NH 2 Allophycocyanin Labeling Kit-SH Kit for Labeling of Proteins/Antibodies with B-Phycoerythrin Kit for Labeling of Proteins/Antibodies with B-Phycoerythrin Kit for Labeling of Proteins/Antibodies with R-Phycoerythrin Kit for Labeling of Proteins/Antibodies with R-Phycoerythrin Kit for Labeling of Proteins/Antibodies with Allophycocyanin Kit for Labeling of Proteins/Antibodies with Allophycocyanin 3 samples PK-PF-BPE-LK22** 3 samples PK-PF-BPE-LK25** 3 samples PK-PF-RPE-LK23** 3 samples PK-PF-RPE-LK26** 3 samples PK-PF-APC-LK21** 3 samples PK-PF-APC-LK24** n Western Blotting Kits and Reagents PromoKine s ECL Chemiluminescence Detection Kit is a convenient, nonradioactive detection system containing all reagents required for highly sensitive chemiluminescent detection of immobilized proteins (Western blotting) or immobilized nucleic acids (Souuthern Blotting or Northern Blotting) conjugated with HRP (Horse Radish Peroxidase). This ready-to-use kit allows the detection of membrane immobilized specific antigens or nucleic acid sequences directly labeled with HRP or indirectly labeled with HRPtagged antibodies/streptavidin/biotin. Product Name Product Description Size Catalog Number PromoKine ECL Chemiluminescence Detection Kit Kit for Chemiluminescent Detection of HRP or HRP-labeled Biomolecules (10 ml are sufficient for one miniblot) 120 ml 500 ml PK-MB PK-MB PromoKine Super-Block PromoKine Hybridization Solution Blocking Reagent for Western, Northern & Southern Blotting Hybridization Solution for Northern & Southern Blotting 10 g PK-MB ml PK-MB Abbreviations: A: activating, aa: amino acid, BA: biologically active, CS: cytotoxic & staining antibody, CT: C-terminus, E: ELISA, ED: extracellular domain, EL: extracellular loop, FC: flow cytometry, FST (AI): functional studies (apoptosis induction), FST (BL): functional studies (blocking), ID: intracellular domain, IF: immunofluorescence microscopy, IHC: immunohistochemistry, IN: intermediate domain, IP: immunoprecipitation, IS: intracellular staining, NT: N-terminus, P: purified by affinity or ion exchange chromatography, WB: Western blot * Other sizes available on request PF-xxx- stands for the respective PromoFluor dye (PF-415, PF-488, PF-505, PF-546, PF-555, PF-590, PF-610, PF-633, PF-647, PF-680, PF-700, PF-750, PF-780 etc.). For information regarding PromoFluor fluorescence ldyes please see Chapter 5.

156 ELISA Kits PromoKine offers a range of highly specific and sensitive ELISA kits that meet the highest quality standards. They can be used for reliable and reproducible detection and quantitation of numerous biomolecules in cell biology research. The 96 assay plate can either be used at once or a desired number of assays can be used because the wells in the assay plates can be detached. In addition, we offer ELISA Development Kits which are very economical in medium- to high-throughput applications. The kits are intended for experienced ELISA users and contain all required reagents to assay your target protein/ peptide in ten 96-well microtiter plates (approximately 1,000 assays). n Cell Signaling: Cytokines, Growth Factors, Receptors and Ligands Product Name Product Description Size Catalog Number 6-Ckine ELISA Kit, mouse Mouse 6-Ckine ELISA 96 tests PK-EL-66205M 90K (Mac-2BP) ELISA Kit, human Human Macrophage Antigen 2 Binding Protein ELISA 96 Tests PK-EL Adiponectin ELISA Kit, human Human Adiponectin ELISA 96 tests PK-EL Angiogenin ELISA Kit, human Human Angiogenin ELISA 96 tests PK-EL Angiopoietin-1 ELISA Kit, human Human Angiopoietin 1 ELISA 96 tests PK-EL Angiostatin ELISA Kit, human Human Angiostatin ELISA 96 tests PK-EL Anti-IFN-alpha ELISA Kit, human Human Anti-Interferon alpha ELISA 96 Tests PK-EL APRIL ELISA Kit, human Human APRIL ELISA 96 Tests PK-EL AXL ELISA Kit, human Human AXL ELISA 96 tests PK-EL AXL ELISA Kit, mouse Mouse AXL ELISA 96 tests PK-EL-60080M BAFF ELISA Kit, human Human B-cell Activating Factor in TNF Family (BLyS) ELISA 96 Tests PK-EL-K9410 BDNF ELISA Kit, human Human Brain Derived Neurotrophic Factor ELISA 96 tests PK-EL BLC ELISA Kit, human Human B-Lymphocyte Chemoattractant ELISA 96 tests PK-EL BLC ELISA Kit, mouse Mouse B-Lymphocyte Chemoattractant ELISA 96 tests PK-EL-65240M BMP-4 ELISA Kit, human Human Bone Morphogenetic Protein 4 ELISA 96 tests PK-EL BMP-6 ELISA Kit, human Human Bone Morphogenetic Protein 6 ELISA 96 tests PK-EL BMP-7 ELISA Kit, human Human Bone Morphogenetic Protein 7 ELISA 96 tests PK-EL CD30L ELISA Kit, mouse Mouse soluble CD30 Ligand ELISA 96 tests PK-EL-66596M CD40L ELISA Kit, human Human soluble CD40 Ligand ELISA 96 Tests PK-EL CD40L ELISA Kit, mouse Mouse soluble CD40 Ligand ELISA 96 Tests PK-EL-63106M CNTF ELISA Kit, human Human Ciliary Neurotrophic Factor ELISA 96 tests PK-EL CRG-2 ELISA Kit, mouse Mouse CRG-2 (Cytokine Responsive Gene 2) ELISA 96 tests PK-EL-66598M CRP ELISA Kit, human Human C-Reactive Protein ELISA 96 Tests PK-EL-K9710s CTACK ELISA Kit, mouse Mouse Cuteaneous T-cell Attracting Chemokine (CCL27) ELISA 96 tests PK-EL-66590M CXCL16 ELISA Kit, mouse Mouse CXC Ligand 16 ELISA 96 tests PK-EL-66610M EGF ELISA Kit, human Human Epidermal Growth Factor ELISA 96 tests PK-EL EGF-R (active) ELISA Kit, human EGF-R ELISA Kit, human Human soluble Epidermal Growth Factor Receptor (active) ELISA Human soluble Epidermal Growth Factor Receptor ELISA 96 Tests PK-EL tests PK-EL Eotaxin ELISA Kit, mouse Mouse Eotaxin ELISA 96 tests PK-EL-65320M Eotaxin-2 ELISA Kit, human Human Eotaxin-2 ELISA 96 tests PK-EL EPO ELISA Kit, human Human Erythropoietin ELISA 96 tests PK-EL-60022

157 155 Product Name Product Description Size Catalog Number E-selectin (CD62E) ELISA Kit, human Human soluble E-selectin (CD62E) ELISA 96 Tests PK-EL E-selectin (CD62E) ELISA Kit, monkey Monkey soluble E-selectin (CD62E) ELISA 96 Tests PK-EL-69216MK FGF-b (FGF-2) ELISA Kit, human Human Fibroblast Growth Factor basic (FGF-2) ELISA 96 tests PK-EL FGF-b (FGF-2) ELISA Kit, human Human Fibroblast Growth Factor basic (FGF-2) ELISA 96 tests PK-EL-60240L* FGF-4 ELISA Kit, human Human Fibroblast Growth Factor 4 ELISA 96 tests PK-EL FGF-6 ELISA Kit, human Human Fibroblast Growth Factor 6 ELISA 96 tests PK-EL FGF-9 ELISA Kit, human Human Fibroblast Growth Factor 9 ELISA 96 tests PK-EL flt-3 Ligand ELISA Kit, human Human flt-3 Ligand ELISA 96 tests PK-EL Fractalkine ELISA Kit, human Human Fractalkine ELISA 96 tests PK-EL Fractalkine ELISA Kit, rat Rat Fractalkine ELISA 96 tests PK-EL-67510R Fractalkine ELISA Kit, rat Rat Fractalkine ELISA 96 tests PK-EL-67510RL* Galectin-3 ELISA Kit, human Human Galectin-3 (Mac-2) ELISA 96 Tests PK-EL G-CSF ELISA Kit, human Human Granulocyte Colony Stimulating Factor ELISA 96 tests PK-EL G-CSF ELISA Kit, mouse Mouse Granulocyte Colony Stimulating Factor ELISA 96 tests PK-EL-60434M GITR ELISA Kit, human Human GITR ELISA 96 tests PK-EL GITR ELISA Kit, human Human GITR ELISA 96 tests PK-EL-60510L* GM-CSF ELISA Kit, human GM-CSF ELISA Kit, mouse GM-CSF ELISA Kit, rat Human Granulocyte/Macrophage Colony Stimulating Factor ELISA Mouse Granulocyte/Macrophage Colony Stimulating Factor ELISA Rat Granulocyte/Macrophage Colony Stimulating Factor ELISA 96 Tests PK-EL Tests PK-EL-60406M 96 Tests PK-EL-60406R HCC-4 ELISA Kit, human Human Hemofiltrate CC-Chemokine 4 ELISA 96 tests PK-EL HGF ELISA Kit, human Human Hepatocyte Growth Factor ELISA 96 tests PK-EL HGF ELISA Kit, human Human Hepatocyte Growth Factor ELISA 96 tests PK-EL-64530L* ICAM-1 (scd54) ELISA Kit, human Human soluble Intercellular Adhesion Molecule 1 (CD54) ELISA ICAM-1 (scd54) ELISA Kit, monkey Monkey soluble Intercellular Adhesion Molecule 1 (CD54) ELISA ICAM-2 (scd102) ELISA Kit, human Human soluble Intercellular Adhesion Molecule 2 (CD102) ELISA ICAM-3 (scd50) ELISA Kit, human Human soluble Intercellular Adhesion Molecule 3 (CD50) ELISA 96 Tests PK-EL Tests PK-EL-63356MK 96 Tests PK-EL Tests PK-EL IFN-alpha ELISA Kit, human Human Interferon alpha ELISA 96 Tests PK-EL IFN-gamma ELISA Kit, human Human Interferon gamma ELISA 96 Tests PK-EL IFN-gamma ELISA Kit, human Human Interferon gamma ELISA 96 tests PK-EL-60707L* IFN-gamma ELISA Kit, mouse Mouse Interferon gamma ELISA 96 Tests PK-EL-60706M IFN-gamma ELISA Kit, mouse Mouse Interferon gamma ELISA 96 tests PK-EL-60707ML* IFN-gamma ELISA Kit, pig Pig Interferon gamma ELISA 96 Tests PK-EL-60706P IFN-gamma ELISA Kit, rat Rat Interferon gamma ELISA 96 Tests PK-EL-60706R IFN-omega ELISA Kit, human Human Interferon omega ELISA 96 Tests PK-EL IGF-BP1 ELISA Kit, human Human Insulin Like Growth Factor Binding Protein 1 ELISA 96 tests PK-EL * For cell and tissue lysate

158 156 Product Name Product Description Size Catalog Number IGF-BP2 ELISA Kit, human Human Insulin Like Growth Factor Binding Protein 2 ELISA IGF-BP3 ELISA Kit, human Human Insulin Like Growth Factor Binding Protein 3 ELISA IGF-BP4 ELISA Kit, human Human Insulin Like Growth Factor Binding Protein 4 ELISA IGF-BP6 ELISA Kit, mouse Mouse Insulin Like Growth Factor Binding Protein 6 ELISA 96 tests PK-EL tests PK-EL tests PK-EL tests PK-EL-60853M IGF-I ELISA Kit, human Human Insulin Like Growth Factor I ELISA 96 tests PK-EL IL-1 alpha ELISA Kit, human Human Interleukin 1-alpha ELISA 96 Tests PK-EL IL-1 alpha ELISA Kit, human Human Interleukin 1-alpha ELISA 96 tests PK-EL-61107L* IL-1 alpha ELISA Kit, mouse Mouse Interleukin 1-alpha ELISA 96 Tests PK-EL-61106M IL-1 alpha ELISA Kit, rat Rat Interleukin 1-alpha ELISA 96 Tests PK-EL-61106R IL-1 beta ELISA Kit, human Human Interleukin 1-beta ELISA 96 Tests PK-EL IL-1 beta ELISA Kit, monkey Monkey Interleukin 1-beta ELISA 96 Tests PK-EL-61126MK IL-1 beta ELISA Kit, mouse Mouse Interleukin 1 beta ELISA 96 tests PK-EL-61127ML* IL-1 beta ELISA Kit, mouse Mouse Interleukin 1-beta ELISA 96 Tests PK-EL-61126M IL-1 beta ELISA Kit, rat Rat Interleukin 1-beta ELISA 96 Tests PK-EL-61126R IL-1 RA ELISA Kit, human Human Interleukin 1 Receptor Antagonist ELISA 96 tests PK-EL IL-1 RA ELISA Kit, human Human Interleukin 1 Receptor Antagonist ELISA 96 tests PK-EL-61122L* IL-2 ELISA Kit, human Human Interleukin 2 ELISA 96 Tests PK-EL IL-2 ELISA Kit, monkey Monkey Interleukin 2 ELISA 96 Tests PK-EL-61206MK IL-2 ELISA Kit, mouse Mouse Interleukin 2 ELISA 96 Tests PK-EL-61206M IL-2 ELISA Kit, rat Rat Interleukin 2 ELISA 96 Tests PK-EL-61206R IL-2R ELISA Kit, human Human soluble Interleukin 2 Receptor ELISA 96 Tests PK-EL IL-4 ELISA Kit, human Human Interleukin 4 ELISA 96 Tests PK-EL IL-4 ELISA Kit, mouse Mouse Interleukin 4 ELISA 96 Tests PK-EL-61406M IL-4 ELISA Kit, rat Rat Interleukin 4 ELISA 96 Tests PK-EL-61406R IL-5 ELISA Kit, human Human Interleukin 5 ELISA 96 Tests PK-EL IL-5 ELISA Kit, mouse Mouse Interleukin 5 ELISA 96 Tests PK-EL-61506M IL-6 ELISA Kit, human Human Interleukin 6 ELISA 96 Tests PK-EL IL-6 ELISA Kit, human Human Interleukin 6 ELISA 96 tests PK-EL-61607L* IL-6 ELISA Kit, monkey Monkey Interleukin 6 ELISA 96 Tests PK-EL-61606MK IL-6 ELISA Kit, mouse Mouse Interleukin 6 ELISA 96 Tests PK-EL-61606M IL-6 ELISA Kit, mouse Mouse Interleukin 6 ELISA 96 tests PK-EL-61607ML* IL-6 ELISA Kit, rat Rat Interleukin 6 ELISA 96 Tests PK-EL-61606R IL-6R ELISA Kit, human Human soluble Interleukin 6 Receptor ELISA 96 Tests PK-EL IL-7 ELISA Kit, human Human Interleukin 7 ELISA 96 tests PK-EL IL-7 ELISA Kit, mouse Mouse Interleukin 7 ELISA 96 tests PK-EL-61712M IL-8 ELISA Kit, human Human Interleukin 8 ELISA 96 Tests PK-EL IL-8 ELISA Kit, human Human Interleukin 8 ELISA 96 tests PK-EL-61807L* * For cell and tissue lysate

159 157 Product Name Product Description Size Catalog Number IL-8 ELISA Kit, monkey Monkey Interleukin 8 ELISA 96 Tests PK-EL-61806MK IL-9 ELISA Kit, mouse Mouse Interleukin 9 ELISA 96 tests PK-EL-61920M IL-10 ELISA Kit, human Human Interleukin 10 ELISA 96 Tests PK-EL IL-10 ELISA Kit, monkey Monkey Interleukin 10 ELISA 96 Tests PK-EL-62006MK IL-10 ELISA Kit, mouse Mouse Interleukin 10 ELISA 96 Tests PK-EL-62006M IL-10 ELISA Kit, rat Rat Interleukin 10 ELISA 96 Tests PK-EL-62006R IL-11 ELISA Kit, human Human Interleukin 11 ELISA 96 tests PK-EL IL-12 (p40 + p70) ELISA Kit, mouse Mouse Interleukin 12 (p40 + p70) ELISA 96 Tests PK-EL-62206M IL-12 (p40) / IL-23 ELISA Kit, human Human Interleukin 12 (p40) / Interleukin 23 ELISA 96 tests PK-EL IL-12 (p70) ELISA Kit, human Human Interleukin 12 (p70) ELISA 96 Tests PK-EL IL-12 (p70) ELISA Kit, monkey Monkey Interleukin 12 (p70) ELISA 96 Tests PK-EL-62216MK IL-12 (p70) ELISA Kit, mouse Mouse Interleukin 12 (p70) ELISA 96 Tests PK-EL-62216M IL-13 ELISA Kit, human Human Interleukin 13 ELISA 96 Tests PK-EL IL-13 ELISA Kit, mouse Mouse Interleukin 13 ELISA 96 tests PK-EL-62306M IL-15 ELISA Kit, human Human Interleukin 15 ELISA 96 tests PK-EL IL-17 ELISA Kit, human Human Interleukin 17 ELISA 96 tests PK-EL IL-17 ELISA Kit, mouse Mouse Interleukin 17 ELISA 96 Tests PK-EL-62716M IL-18 ELISA Kit, pig Pig Interleukin 18 ELISA 96 Tests PK-EL-62816P IL-21 ELISA Kit, human Human Interleukin 21 ELISA 96 tests PK-EL IL-22 ELISA Kit, human Human Interleukin 22 ELISA 96 tests PK-EL IL-22 ELISA Kit, mouse Mouse Interleukin 22 ELISA 96 tests PK-EL-62922M IL-23 ELISA Kit, human Human Interleukin 23 ELISA 96 Tests PK-EL IP-10 ELISA Kit, human Human IFN Inducible Protein 10 (CXCL10) ELISA 96 tests PK-EL IP-10 ELISA Kit, mouse Mouse IFN Inducible Protein 10 (CXCL10) ELISA 96 tests PK-EL-65520M Leptin ELISA Kit, human Human Leptin ELISA 96 tests PK-EL Leptin ELISA Kit, mouse Mouse Leptin ELISA 96 tests PK-EL-68232M LIF ELISA Kit, human Human Leukocyte Inhibitory Factor ELISA 96 Tests PK-EL LIF-R (gp190) ELISA Kit, human Human soluble Leukocyte Inhibitory Factor Receptor (gp190) ELISA 96 Tests PK-EL LIGHT ELISA Kit, human Human LIGHT (TNFSF14) ELISA 96 Tests PK-EL LIX ELISA Kit, rat LIX ELISA Kit, rat Rat Lipopolysaccharide Induced CXC Chemokine (CXCL5) ELISA Rat Lipopolysaccharide Induced CXC Chemokine (CXCL5) ELISA 96 tests PK-EL-66519R 96 tests PK-EL-66519RL* L-selectin ELISA Kit, human Human soluble L-selectin ELISA 96 Tests PK-EL MCP-1 ELISA Kit, human Human Monocyte Chemotactic Protein 1 (CCL2) ELISA 96 Tests PK-EL MCP-1 ELISA Kit, mouse Mouse Monocyte Chemotactic Protein 1 (CCL2) ELISA 96 Tests PK-EL-64006M MCP-1 ELISA Kit, rat MCP-1 ELISA Kit, rat Rat Monocyte Chemotactic Protein 1 (MCAF, CCL2) ELISA Rat Monocyte Chemotactic Protein 1 (MCAF, CCL2) ELISA 96 tests PK-EL-64006R 96 tests PK-EL-64006RL* * For cell and tissue lysate

160 158 Product Name Product Description Size Catalog Number MCP-2 ELISA Kit, human MCP-5 ELISA Kit, mouse Human Monocyte Chemotactic Protein 2 (CCL8) ELISA Mouse Monocyte Chemotactic Protein 5 (CCL12) ELISA 96 tests PK-EL tests PK-EL-65725M M-CSF ELISA Kit, human Human Macrophage Colony Stimulating Factor ELISA 96 tests PK-EL MDC ELISA Kit, human MIG ELISA Kit, human MIP-1 alpha ELISA Kit, mouse MIP-1 beta ELISA Kit, human MIP-1 delta ELISA Kit, human MIP-2 ELISA Kit, mouse MIP-3 alpha ELISA Kit, human MIP-3 alpha ELISA Kit, human Human Macrophage Derived Chemokine (CCL22) ELISA Human Monokine Induced by Interferon-gamma (CXCL9) ELISA Mouse Macrophage Inflammatory Protein 1-alpha (CCL3) ELISA Human Macrophage Inflammatory Protein 1-beta (CCL4) ELISA Human Macrophage Inflammatory Protein 1-delta ELISA Mouse Macrophage Inflammatory Protein 2 (CXCL2) ELISA Human Macrophage Inflammatory Protein 3-alpha (CCL20) ELISA Human Macrophage Inflammatory Protein 3-alpha (CCL20) ELISA 96 tests PK-EL tests PK-EL tests PK-EL-65833M 96 tests PK-EL tests PK-EL tests PK-EL-65835M 96 tests PK-EL tests PK-EL-65866L* MMP-1 ELISA Kit, human Human Matrix Metalloproteinase 1 ELISA 96 tests PK-EL MMP-3 ELISA Kit, human Human Matrix Metalloproteinase 3 ELISA 96 tests PK-EL MMP-8 ELISA Kit, human Human Matrix Metalloproteinase 8 ELISA 96 tests PK-EL MMP-9 ELISA Kit, human Human Matrix Metalloproteinase 9 ELISA 96 Tests PK-EL MMP-10 ELISA Kit, human Human Matrix Metalloproteinase 10 ELISA 96 tests PK-EL MMP-13 ELISA Kit, human Human Matrix Metalloproteinase 13 ELISA 96 Tests PK-EL MPO ELISA Kit, human Human Myeloperoxidase (MPO) ELISA 96 Tests PK-EL-K6631 MSP ELISA Kit, human Human Macrophage Stimulating Protein ELISA 96 tests PK-EL Myostatin ELISA Kit, human Human Myostatin ELISA 96 Tests PK-EL-K1012 NAP-2 ELISA Kit, human Human Neutrophil Activating Protein 2 (CXCL7) ELISA 96 tests PK-EL NT-3 ELISA Kit, human Human Neurotrophin 3 ELISA 96 tests PK-EL OPG ELISA Kit, human Human Osteoprotegerin (OPG) ELISA 96 Tests PK-EL-KB1011 p185 (HER-2) ELISA Kit, human Human soluble p185 (EGF Receptor 2) ELISA 96 Tests PK-EL PAI-1 ELISA Kit, human Human Plasminogen Activator Inhibitor 1 ELISA 96 tests PK-EL PARC ELISA Kit, human Human Pulmonary & Activation Regulated Chemokine ELISA 96 tests PK-EL PDGF-BB ELISA Kit, human Human Platelet Derived Growth Factor BB ELISA 96 tests PK-EL PDGF-BB ELISA Kit, human Human Platelet Derived Growth Factor BB ELISA 96 tests PK-EL-63022L* PECAM-1 (scd31) ELISA Kit, human PECAM-1 (scd31) ELISA Kit, monkey Human soluble Platelet Endothelial Cell Adhesion Molecule 1 (CD31) ELISA Monkey soluble Platelet Endothelial Cell Adhesion Molecule 1 (CD31) ELISA 96 Tests PK-EL Tests PK-EL-63339MK PMN-Elastase ELISA Kit, human Human PMN-Elastase ELISA 96 Tests PK-EL * For cell and tissue lysate

161 159 Product Name Product Description Size Catalog Number P-selectin ELISA Kit, human Human soluble P-selectin ELISA 96 Tests PK-EL P-selectin ELISA Kit, mouse Mouse soluble P-selectin ELISA 96 tests PK-EL-69220M RANTES ELISA Kit, human Human RANTES (CCL5) ELISA 96 tests PK-EL Resistin ELISA Kit, human Human Resistin ELISA 96 tests PK-EL SCF ELISA Kit, human Human Stem Cell Factor ELISA 96 tests PK-EL SCF ELISA Kit, mouse Mouse Stem Cell Factor ELISA 96 tests PK-EL-63120M SDF-1 alpha ELISA Kit, human Human Stromal Cell Derived Factor 1-alpha ELISA 96 tests PK-EL SDF-1 alpha ELISA Kit, mouse Mouse Stromal Cell Derived Factor 1-alpha ELISA 96 tests PK-EL-67220M sgp130 ELISA Kit, human Human sgp130 ELISA 96 tests PK-EL srankl (total) ELISA Kit, human srankl ELISA Kit, human Human soluble Receptor Activator of NF-Kappa-B Ligand (total) ELISA Human soluble Receptor Activator of NF-Kappa-B Ligand ELISA 96 Tests PK-EL-K Tests PK-EL-KB20452 TECK ELISA Kit, mouse Mouse Thymus-Expressed Chemokine (CCL25) ELISA 96 tests PK-EL-68020M TGF-alpha ELISA Kit, human Human Transforming Growth Factor alpha ELISA 96 tests PK-EL TGF-beta 1 ELISA Kit, human Human Transforming Growth Factor beta-1 ELISA 96 Tests PK-EL TGF-beta 1 ELISA Kit, mouse Mouse Transforming Growth Factor beta-1 ELISA 96 Tests PK-EL-63506M TGF-beta 1 ELISA Kit, rat Rat Transforming Growth Factor beta-1 ELISA 96 Tests PK-EL-63506R TGF-beta 2 ELISA Kit, human Human Transforming Growth Factor beta-2 ELISA 96 Tests PK-EL TIE-2 ELISA Kit, human Human TIE-2 ELISA 96 tests PK-EL TIMP-1 ELISA Kit, human Human Tissue Inhibitor of Matrix Metalloprotease 1 ELISA TIMP-1 ELISA Kit, mouse Mouse Tissue Inhibitor of Matrix Metalloprotease 1 ELISA 96 tests PK-EL tests PK-EL-68559M TIMP-1 ELISA Kit, rat Rat Tissue Inhibitor of Matrix Metalloprotease 1 ELISA 96 tests PK-EL-68559R TIMP-1 ELISA Kit, rat Rat Tissue Inhibitor of Matrix Metalloprotease 1 ELISA 96 tests PK-EL-68559RL* TIMP-2 ELISA Kit, human Human Tissue Inhibitor of Matrix Metalloprotease 2 ELISA TIMP-2 ELISA Kit, mouse Mouse Tissue Inhibitor of Matrix Metalloprotease 2 ELISA 96 tests PK-EL tests PK-EL-68561M TNF-alpha (total) ELISA Kit, human Human Tumor Necrosis Factor alpha (total) ELISA 96 tests PK-EL TNF-alpha ELISA Kit, human Human Tumor Necrosis Factor alpha ELISA 96 Tests PK-EL TNF-alpha ELISA Kit, monkey Monkey Tumor Necrosis Factor alpha ELISA 96 Tests PK-EL-63706MK TNF-alpha ELISA Kit, mouse Mouse Tumor Necrosis Factor alpha ELISA 96 Tests PK-EL-63706M TNF-alpha ELISA Kit, rat Rat Tumor Necrosis Factor alpha ELISA 96 Tests PK-EL-63706R TNF-beta ELISA Kit, human Human Tumor Necrosis Factor beta ELISA 96 Tests PK-EL TNF-R (60 kda) ELISA Kit, human Human soluble Tumor Necrosis Factor Receptor (60 kda) ELISA TNF-R (80 kda) ELISA Kit, human Human soluble Tumor Necrosis Factor Receptor (80 kda) ELISA TNF-R I ELISA Kit, monkey Monkey soluble Tumor Necrosis Factor Receptor I ELISA 96 Tests PK-EL Tests PK-EL Tests PK-EL-63729MK * For cell and tissue lysate

162 160 Product Name Product Description Size Catalog Number TNF-R II ELISA Kit, human Human soluble Tumor Necrosis Factor Receptor II ELISA 96 tests PK-EL t-pa ELISA Kit, human Human Tissue-type Plasminogen Activator ELISA 96 Tests PK-EL TPO ELISA Kit, human Human Thrombopoietin ELISA 96 tests PK-EL TPO ELISA Kit, mouse Mouse Thrombopoietin ELISA 96 tests PK-EL-65112M TRAIL ELISA Kit, human Human TNF-Related Apoptosis Inducing Ligand ELISA 96 Tests PK-EL VAP-1 ELISA Kit, human Human soluble Vascular Apoptosis-Inducing Protein 1 ELISA VCAM-1 / scd106 ELISA Kit, human Human soluble Vascular Cellular Adhesion Molecule 1 (CD106) ELISA VCAM-1 / scd106 ELISA Kit, mouse VEGF-A ELISA Kit, human VEGF-A ELISA Kit, human VEGF-A ELISA Kit, mouse VEGF-A ELISA Kit, mouse Mouse Vascular Cellular Adhesion Molecule 1 (CD106) ELISA Human Vascular Endothelial Cell Growth Factor A ELISA Human Vascular Endothelial Cell Growth Factor A ELISA Mouse Vascular Endothelial Cell Growth Factor A ELISA Mouse Vascular Endothelial Cell Growth Factor A ELISA 96 tests PK-EL Tests PK-EL tests PK-EL-60062M 96 Tests PK-EL tests PK-EL-64407L* 96 Tests PK-EL-64406M 96 tests PK-EL-64407ML* VEGF-A ELISA Kit, rat Rat Vascular Endothelial Cell Growth Factor A ELISA 96 tests PK-EL-64407R VEGF-A ELISA Kit, rat Rat Vascular Endothelial Cell Growth Factor A ELISA 96 tests PK-EL-64407RL* VEGF-C ELISA Kit, human Human Vascular Endothelial Cell Growth Factor C ELISA 96 Tests PK-EL VEGF-C ELISA Kit, rat Rat Vascular Endothelial Cell Growth Factor C ELISA 96 Tests PK-EL-64416R VEGF-R I ELISA Kit, human VEGF-R II ELISA Kit, human Human soluble Vascular Endothelial Growth Factor Receptor I ELISA Human Vascular Endothelial Cell Growth Factor Receptor II ELISA 96 Tests PK-EL tests PK-EL n CD Marker Product Name Product Description Size Catalog Number scd8 ELISA Kit, human Human soluble CD8 ELISA 96 Tests PK-EL scd23 ELISA Kit, human Human soluble CD23 ELISA 96 Tests PK-EL scd26 ELISA Kit, human Human soluble CD26 ELISA 96 Tests PK-EL scd28 ELISA Kit, human Human soluble CD28 ELISA 96 Tests PK-EL scd30 ELISA Kit, human Human soluble CD30 ELISA 96 Tests PK-EL scd31 (specam-1) ELISA Kit, human scd31 (specam-1) ELISA Kit, monkey Human soluble CD31 (soluble Platelet Endothelial Cell Adhesion Molecule 1) ELISA Monkey soluble CD31 (soluble Platelet Endothelial Cell Adhesion Molecule 1) ELISA 96 Tests PK-EL Tests PK-EL-63339MK scd40 ELISA Kit, human Human soluble CD40 ELISA 96 Tests PK-EL scd44std ELISA Kit, human Human soluble CD44 (standard) ELISA 96 Tests PK-EL * For cell and tissue lysate

163 161 Product Name Product Description Size Catalog Number scd44var (v5) ELISA Kit, human Human soluble CD44 (variant 5) ELISA 96 Tests PK-EL scd44var (v6) ELISA Kit, human Human soluble CD44 (variant 6) ELISA 96 Tests PK-EL scd50 (sicam-3) ELISA Kit, human scd54 (sicam-1) ELISA Kit, human scd54 (sicam-1) ELISA Kit, monkey Human soluble CD50 (soluble Intercellular Adhesion Molecule 3) ELISA Human soluble CD54 (soluble Intercellular Adhesion Molecule 1) ELISA Monkey soluble CD54 (soluble Intercellular Adhesion Molecule 1) ELISA 96 Tests PK-EL Tests PK-EL Tests PK-EL-63356MK scd62e (se-selectin) ELISA Kit, human Human soluble CD62E (soluble E-selectin) ELISA 96 Tests PK-EL scd62p (sp-selectin) ELISA Kit, human Human soluble CD62P (soluble P-selectin) ELISA 96 Tests PK-EL scd102 (sicam-2) ELISA Kit, human scd106 (svcam-1) ELISA Kit, human Human soluble CD102 (soluble Intercellular Adhesion Molecule 2) ELISA Human soluble CD106 (soluble Vascular Cellular Adhesion Molecule 1) ELISA 96 Tests PK-EL Tests PK-EL scd134 (OX-40) ELISA Kit, human Human soluble CD134 (OX-40) ELISA 96 Tests PK-EL scd137 (4-1BB) ELISA Kit, human Human soluble CD137 (4-1BB) ELISA 96 Tests PK-EL scd144 (sve-cadherin) ELISA Kit, human scd152 (sctla-4) ELISA Kit, human Human soluble CD144 (soluble VE-Cadherin) ELISA 96 Tests PK-EL Human soluble CD152 (soluble Cytotoxic T-lymphocyte Associated Antigen 4) ELISA 96 Tests PK-EL scd153 (scd30l) ELISA Kit, human Human soluble CD153 (soluble CD30 Ligand) ELISA 96 Tests PK-EL scd162 (spsgl-1) ELISA Kit, human Human soluble CD162 (soluble P-selectin Glycoprotein Ligand 1) ELISA 96 Tests PK-EL n Hormones Product Name Product Description Size Catalog Number Relaxin ELISA Kit, human Human Relaxin ELISA 96 Tests PK-EL-K9210 Thymosin a1 ELISA Kit, human Human Thymosin alpha-1 ELISA 96 Tests PK-EL-K9510 Thymosin b4 ELISA Kit, human Human Thymosin beta-4 ELISA 96 Tests PK-EL-K9520 Thymulin ELISA Kit, human Human Thymulin ELISA 96 Tests PK-EL-K9810 n Apoptosis Product Name Product Description Size Catalog Number Annexin V ELISA Kit, human Human Annexin V ELISA 96 Tests PK-EL Anti-Annexin V ELISA Kit, human Human anti-annexin V ELISA 96 Tests PK-EL APRIL ELISA Kit, human Human APRIL ELISA 96 Tests PK-EL BAFF ELISA Kit, human Human B-cell Activating Factor in TNF Family (BLyS) ELISA 96 Tests PK-EL-K9410 Bcl-2 ELISA Kit, human Human B-cell Lymphoma/Leukemia 2 ELISA 96 Tests PK-EL * For cell and tissue lysate

164 162 Product Name Product Description Size Catalog Number Caspase-8 / FLICE ELISA Kit, human Human Caspase 8/FLICE ELISA 96 Tests PK-EL Caspase-9 ELISA Kit, human Human Caspase 9 ELISA 96 Tests PK-EL CD30 ELISA Kit, human Human soluble CD30 ELISA 96 Tests PK-EL CD40 ELISA Kit, human Human soluble CD40 ELISA 96 Tests PK-EL CD40L ELISA Kit, human Human soluble CD40 Ligand ELISA 96 Tests PK-EL Cu/ZnSOD ELISA Kit, human Human Copper/Zinc Superoxide Dismutase ELISA 96 Tests PK-EL Cytochrome c ELISA Kit, human Human Cytochrome c ELISA 96 Tests PK-EL DcR3 ELISA Kit, human Human Decoy Receptor 3 ELISA 96 Tests PK-EL Fas / APO-1 ELISA Kit, human Human soluble Fas (Apoptosis Antigen 1) ELISA 96 Tests PK-EL Fas / APO-1 ELISA Kit, human Human soluble Fas ELISA 96 tests PK-EL-69207L* Fas Ligand ELISA Kit, human Human soluble Fas Ligand ELISA 96 Tests PK-EL Granzyme A ELISA Kit, human Human Granzyme A ELISA 96 Tests PK-EL Granzyme B ELISA Kit, human Human Granzyme B ELISA 96 Tests PK-EL OPG ELISA Kit, human Human Osteoprotegerin (OPG) ELISA 96 Tests PK-EL-KB1011 OX-40 (CD134) ELISA Kit, human Human CD134 (OX-40) ELISA 96 Tests PK-EL TRAIL ELISA Kit, human Human TNF-Related Apoptosis Inducing Ligand ELISA 96 Tests PK-EL VE-Cadherin ELISA Kit, monkey Monkey soluble VE-Cadherin ELISA 96 Tests PK-EL-60266MK VE-Cadherin ELISA Kit, human Human soluble VE-Cadherin ELISA 96 Tests PK-EL n Other ELISAs Product Name Product Description Size Catalog Number 1,25-Dihydroxy Vitamin D ELISA Kit, human 25-OH Vitamin D (direct) ELISA Kit, human Human 1,25-(OH) 2 Vitamin D ELISA 48 Tests PK-EL-K2112 Human 25-OH Vitamin D (direct) ELISA 96 tests PK-EL-K2109 a1-antitrypsin (a1-proteinase Inhibitor) ELISA Kit, human a1-microglobulin (a1-m) ELISA Kit, human Human alpha1-antitrypsin (alpha1-proteinase Inhibitor) ELISA 96 Tests PK-EL-K6750 Human alpha1-mikroglobulin (a1-m) ELISA 96 Tests PK-EL-K6710 a2-macroglobulin ELISA Kit, human Human alpha2-macroglobulin (a2-m) ELISA 96 tests PK-EL-K6610 ADMA direct ELISA Kit, human Human Asymetric Dimethyl Arginine (direct) ELISA 96 Tests PK-EL-K7812 ADMA direct ELISA Kit, mouse/rat b2-microglobulin (b2-m) ELISA Kit, human Mouse/Rat ADMA direct (Asymetric Dimethyl Arginine) ELISA 96 tests PK-EL-K3001 Human beta2-mikroglobulin (b2-m) ELISA 96 Tests PK-EL Big Endothelin ELISA Kit, human Human Big Endothelin ELISA 96 Tests PK-EL-KB20082 BSP ELISA Kit, human Human Bone Sialoprotein (BSP) ELISA 96 Tests PK-EL-K4220 Calprotectin (MRP 8/14) ELISA Kit, human Human Calprotectin (MRP 8/14) ELISA 96 Tests PK-EL-K6935 Cathepsin K ELISA Kit, human Human Cathepsin K ELISA 96 Tests PK-EL-KB20432 * For cell and tissue lysate

165 163 Product Name Product Description Size Catalog Number Cathepsin L ELISA Kit, human Human Cathepsin L ELISA 96 Tests PK-EL DKK-1 ELISA, human Human DKK-1 (Dickkopf-1) ELISA Kit 96 tests PK-EL-KB20412 EDN (EPX) ELISA Kit, human Human Eosinophil Derived Neurotoxin (EPX) ELISA 96 Tests PK-EL-K6810 Endothelin 1/2 ELISA Kit, human Human Endothelin 1/2 ELISA 96 Tests PK-EL-KB1140 Fibronectin ELISA Kit, human Human Fibronectin ELISA 96 Tests PK-EL Galectin-3 ELISA Kit, human Human Galectin-3 (Mac-2) ELISA 96 Tests PK-EL Immunglobulin E [IgE] ELISA Kit, human Human Immunglobulin E [IgE] ELISA 96 tests PK-EL-K6511 Immunglobulin G [IgG] ELISA Kit, human Human Immunglobulin G [IgG] ELISA 96 tests PK-EL-K6510 MGP ELISA Kit, human Human Matrix Gla Protein ELISA 96 tests PK-EL-KB20062 Microglobulin ELISA Kit, human Human Microglobulin ELISA 96 tests PK-EL NT-proANP 1-98 ELISA Kit, human Human Atrial Natriuretic propeptid (proanp 1-98) ELISA 96 Tests PK-EL-KB20892 NT-proCNP ELISA Kit, human Human C-Type Natriuretic Propeptid (NT-proCNP) ELISA 96 Tests PK-EL-KB20872 Oxidized LDL (oxldl) ELISA Kit, human Human Oxidized LDL (oxldl) ELISA 96 Tests PK-EL-K7810 p53 ELISA Kit, human Human p53 ELISA 96 Tests PK-EL RBP ELISA Kit, human Human Retinol Binding Protein (RBP) ELISA 96 Tests PK-EL-K6110 Thyreoglobulin (htg) ELISA Kit, human Human Thyreoglobulin (htg) ELISA 96 Tests PK-EL-K7510 Thyreoglobulin (htg, recovery) ELISA Kit, human Human Thyreoglobulin (htg, recovery) ELISA 96 Tests PK-EL-K7510A Urotensin II ELISA Kit, human Human Urotensin II ELISA 96 Tests PK-EL-K1013 VDBP ELISA Kit, human Human Vitamin D Binding Protein ELISA 96 Tests PK-EL-K2314 Zonulin ELISA Kit, human Human Zonulin ELISA 96 tests PK-EL-K5600 n ELISA Development Kits Product Name Product Description Size Catalog Number BD-2 EDK, human Human beta-defensin 2 ELISA Development Kit 1000 tests PK-EL-65250D BD-3 EDK, human Human beta-defensin 3 ELISA Development Kit 1000 tests PK-EL-65258D Betacellulin EDK, human Human Betacellulin ELISA Development Kit 1000 tests PK-EL-67360D BMP-2 EDK, human Human Bone Morphogenetic Protein 2 ELISA Development Kit 1000 tests PK-EL-67310D scd40l EDK, human Human scd40 Ligand ELISA Development Kit 1000 tests PK-EL-63100D CTGF EDK, human Human Connective Tissue Growth Factor ELISA Development Kit 1000 tests PK-EL-68014D CXCL16 EDK, human Human CXC Ligand 16 ELISA Development Kit 1000 tests PK-EL-66610D EGF EDK, human EGF EDK, mouse Human Epidermal Growth Factor ELISA Development Kit Mouse Epidermal Growth Factor ELISA Development Kit 1000 tests PK-EL-60170D 1000 tests PK-EL-60170DM Eotaxin (CCL11) EDK, human Human Eotaxin (CCL11) ELISA Development Kit 1000 tests PK-EL-65320D * For cell and tissue lysate

166 164 Product Name Product Description Size Catalog Number Eotaxin-3 EDK, human Human Eotaxin-3 ELISA Development Kit 1000 tests PK-EL-65340D FGF-b (FGF-2) EDK, human Human Fibroblast Growth Factor basic (FGF-2) ELISA Development Kit 1000 tests PK-EL-60240D Follistatin EDK, human Human Follistatin ELISA Development Kit 1000 tests PK-EL-67720D G-CSF EDK, human G-CSF EDK, mouse GM-CSF EDK, human GM-CSF EDK, mouse GRO (KC, CXCL1) EDK, rat GRO-alpha (MGSA, CXCL1) EDK, human GRO-beta (MIP-2, CXCL2) EDK, human Human Granulocyte Colony Stimulating Factor ELISA Development Kit Mouse Granulocyte Colony Stimulating Factor ELISA Development Kit Human Granulocyte/Macrophage Colony Stimulating Factor ELISA Development Kit Mouse Granulocyte/Macrophage Colony Stimulating Factor ELISA Development Kit Rat Growth Regulated Oncogen (KC, CXCL1) ELISA Development Kit Human Growth Regulated Oncogen alpha (MGSA, CXCL1) ELISA Development Kit Human Growth Regulated Oncogen beta (MIP-2, CXCL2) ELISA Development Kit 1000 tests PK-EL-60434D 1000 tests PK-EL-60434DM 1000 tests PK-EL-60420D 1000 tests PK-EL-60420DM 1000 tests PK-EL-65240DR 1000 tests PK-EL-65430D 1000 tests PK-EL-65440D IFN-gamma EDK, human Human Interferon gamma ELISA Development Kit 1000 tests PK-EL-60725D IFN-gamma EDK, mouse Mouse Interferon gamma ELISA Development Kit 1000 tests PK-EL-60725DM IFN-gamma EDK, rat Rat Interferon gamma ELISA Development Kit 1000 tests PK-EL-60725DR IGF-I EDK, mouse Mouse Insulin Like Growth Factor I ELISA Development Kit 1000 tests PK-EL-60840DM IL-1 alpha EDK, human Human Interleukin 1-alpha ELISA Development Kit 1000 tests PK-EL-61112D IL-1 alpha EDK, mouse Mouse Interleukin 1-alpha ELISA Development Kit 1000 tests PK-EL-61112DM IL-1 alpha EDK, rat Rat Interleukin 1-alpha ELISA Development Kit 1000 tests PK-EL-61120DR IL-1 beta EDK, mouse Mouse Interleukin 1-beta ELISA Development Kit 1000 tests PK-EL-61120DM IL-1 beta EDK, rat Rat Interleukin 1-beta ELISA Development Kit 1000 tests PK-EL-61112DR IL-2 EDK, human Human Interleukin 2 ELISA Development Kit 1000 tests PK-EL-61240D IL-2 EDK, mouse Mouse Interleukin 2 ELISA Development Kit 1000 tests PK-EL-61240DM IL-3 EDK, human Human Interleukin 3 ELISA Development Kit 1000 tests PK-EL-61330D IL-3 EDK, mouse Mouse Interleukin 3 ELISA Development Kit 1000 tests PK-EL-61330DM IL-4 EDK, human Human Interleukin 4 ELISA Development Kit 1000 tests PK-EL-61420D IL-4 EDK, mouse Mouse Interleukin 4 ELISA Development Kit 1000 tests PK-EL-61420DM IL-5 EDK, human Human Interleukin 5 ELISA Development Kit 1000 tests PK-EL-61520D IL-6 EDK, human Human Interleukin 6 ELISA Development Kit 1000 tests PK-EL-61620D IL-6 EDK, mouse Mouse Interleukin 6 ELISA Development Kit 1000 tests PK-EL-61620DM IL-6 EDK, rat Rat Interleukin 6 ELISA Development Kit 1000 tests PK-EL-61620DR IL-7 EDK, human Human Interleukin 7 ELISA Development Kit 1000 tests PK-EL-61712D IL-8 EDK, human Human Interleukin 8 ELISA Development Kit 1000 tests PK-EL-61830D IL-9 EDK, human Human Interleukin 9 ELISA Development Kit 1000 tests PK-EL-61920D IL-10 EDK, human Human Interleukin 10 ELISA Development Kit 1000 tests PK-EL-62012D IL-10 EDK, mouse Mouse Interleukin 10 ELISA Development Kit 1000 tests PK-EL-62012DM

167 165 Product Name Product Description Size Catalog Number IL-12 EDK, human Human Interleukin 12 ELISA Development Kit 1000 tests PK-EL-62212D IL-12 EDK, mouse Mouse Interleukin 12 ELISA Development Kit 1000 tests PK-EL-62212DM IL-13 EDK, human Human Interleukin 13 ELISA Development Kit 1000 tests PK-EL-62312D IL-13 EDK, mouse Mouse Interleukin 13 ELISA Development Kit 1000 tests PK-EL-62312DM IL-17A EDK, human Human Interleukin 17A ELISA Development Kit 1000 tests PK-EL-62710D IL-17E EDK, human Human Interleukin 17E ELISA Development Kit 1000 tests PK-EL-62715D IL-20 EDK, human Human Interleukin 20 ELISA Development Kit 1000 tests PK-EL-62920D IL-21 EDK, human Human Interleukin 21 ELISA Development Kit 1000 tests PK-EL-62921D IL-22 EDK, human Human Interleukin 22 ELISA Development Kit 1000 tests PK-EL-62922D IL-31 EDK, human Human Interleukin 31 ELISA Development Kit 1000 tests PK-EL-62950D IP-10 EDK, human I-TAC EDK, human Human IFN Inducible Protein 10 (CXCL10) ELISA Development Kit Human IFN Inducible T-cell alpha Chemoattractant ELISA Development Kit 1000 tests PK-EL-65520D 1000 tests PK-EL-67820D Leptin EDK, mouse Mouse Leptin ELISA Development Kit 1000 tests PK-EL-68232DM MCP-1 (CCL2) EDK, rat MCP-1 (JE, CCL2) EDK, mouse Rat Monocyte Chemotactic Protein 1 (CCL2) ELISA Development Kit Mouse Monocyte Chemotactic Protein 1 (JE, CCL2) ELISA Development Kit MCP-1 (MCAF, CCL2) EDK, human Human Monocyte Chemotactic Protein 1 (MCAF, CCL2) ELISA Development Kit MCP-3 (CCL7) EDK, mouse M-CSF EDK, mouse MDC (CCL22) EDK, mouse Mouse Monocyte Chemotactic Protein 3 (CCL7) ELISA Development Kit Mouse Macrophage Colony Stimulating Factor ELISA Development Kit Mouse Macrophage Derived Chemokine (CCL22) ELISA Development Kit 1000 tests PK-EL-64030DR 1000 tests PK-EL-64030DM 1000 tests PK-EL-64030D 1000 tests PK-EL-65723DM 1000 tests PK-EL-60442DM 1000 tests PK-EL-67920DM Midkine (MK) EDK, human Human Midkine (MK) ELISA Development Kit 1000 tests PK-EL-67950D MIP-1 alpha (CCL3) EDK, human MIP-1 alpha (CCL3) EDK, mouse MIP-1 alpha (CCL3) EDK, rat Human Macrophage Inflammatory Protein 1-alpha (CCL3) ELISA Development Kit Mouse Macrophage Inflammatory Protein 1-alpha (CCL3) ELISA Development Kit Rat Macrophage Inflammatory Protein 1-alpha (CCL4) ELISA Development Kit MIP-2 (GRO-beta, CXCL2) EDK, mouse Mouse Macrophage Inflammatory Protein 2 (GRO-beta, CXCL2) ELISA Development Kit NAP-2 (CXCL7) EDK, human beta-ngf EDK, human Human Neutrophil Activating Protein 2 (CXCL7) ELISA Development Kit Human Nerve Growth Factor beta ELISA Development Kit 1000 tests PK-EL-65833D 1000 tests PK-EL-65833DM 1000 tests PK-EL-65833DR 1000 tests PK-EL-65430DM 1000 tests PK-EL-64320D 1000 tests PK-EL-66130D NOV EDK, human Human NOV ELISA Development Kit 1000 tests PK-EL-64335D PAI-1 EDK, human Human Plasminogen Activator Inhibitor 1 ELISA Development Kit PDGF-BB EDK, human Human Platelet Derived Growth Factor BB ELISA Development Kit 1000 tests PK-EL-63790D 1000 tests PK-EL-63022D

168 166 Product Name Product Description Size Catalog Number PLGF-1 EDK, human Human Placenta Growth Factor 1 ELISA Development Kit 1000 tests PK-EL-66021D srankl EDK, human Human srank Ligand ELISA Development Kit 1000 tests PK-EL-63200D srankl EDK, mouse Mouse srank Ligand ELISA Development Kit 1000 tests PK-EL-63200DM RANTES (CCL5) EDK, human Human RANTES (CCL5) ELISA Development Kit 1000 tests PK-EL-64130D RANTES (CCL5) EDK, mouse Mouse RANTES (CCL5) ELISA Development Kit 1000 tests PK-EL-64130DM RANTES (CCL5) EDK, rat Rat RANTES (CCL5) ELISA Development Kit 1000 tests PK-EL-64130DR Resistin EDK, human Human Resistin ELISA Development Kit 1000 tests PK-EL-66670D SCF EDK, human Human Stem Cell Factor ELISA Development Kit 1000 tests PK-EL-63120D SCF EDK, mouse Mouse Stem Cell Factor ELISA Development Kit 1000 tests PK-EL-63120DM SCF EDK, rat Rat Stem Cell Factor ELISA Development Kit 1000 tests PK-EL-63120DR TACI EDK, human Human Transmembrane Activator and CAML Interactor ELISA Development Kit 1000 tests PK-EL-66690D TL-1A EDK, human Human TL-1A (TNFSF15) ELISA Development Kit 1000 tests PK-EL-66695D TNF-alpha EDK, human TNF-alpha EDK, mouse TNF-alpha EDK, rat TPO (MDGF) EDK, human strail (APO2L) EDK, human TSLP EDK, human Human Tumor Necrosis Factor alpha ELISA Development Kit Mouse Tumor Necrosis Factor alpha ELISA Development Kit Rat Tumor Necrosis Factor alpha ELISA Development Kit Human Thrombopoietin (MDGF) ELISA Development Kit Human soluble TNF-Related Apoptosis Inducing Ligand (APO2L) ELISA Development Kit Human Thymic Stromal Lymphopoietin ELISA Development Kit 1000 tests PK-EL-63720D 1000 tests PK-EL-63720DM 1000 tests PK-EL-63720DR 1000 tests PK-EL-65112D 1000 tests PK-EL-63600D 1000 tests PK-EL-63770D TWEAK EDK, human Human TWEAK ELISA Development Kit 1000 tests PK-EL-63610D VEGF EDK, human VEGF EDK, mouse Human Vascular Endothelial Cell Growth Factor A ELISA Development Kit Mouse Vascular Endothelial Cell Growth Factor A ELISA Development Kit 1000 tests PK-EL-64420D 1000 tests PK-EL-64420DM 6.4 Antigens and Cell Lysates The PromoKine peptides are perfectly suited as blocking reagent in combination with the PromoKine antibodies and as a control in e.g. immunoblotting/ Western blot, ELISA or immunohistochemistry assays. PromoKine also provides a wide range of ready-to-use cell and tissue lysates that can be used as controls in e.g. gel electrophoresis, Western blotting or immunoprecipitation. Moreover, they are also useful in enzymatic activity analysis, protein-protein interaction analysis and tissue specific expression identification. The total tissue and cell line protein lysates are available from human, mouse, and rat origin as well as from a variety of developmental stages. Furthermore, beside lysates from normal tissue also lysates from tumor tissue and diseased tissue are available. For a complete list of our peptides and cell lysates please see our website or the PromoKine Price List.

169 Cytokines and Growth 7 Factors Cytokines and chemokines are small proteins secreted by a variety of cell types. They are important signal messengers mediating cell communication and activating other target cells through binding to specific receptors. Cytokines play a central role in the immune system - in which the chemokines mainly function as chemoattractants. They also coordinate and regulate other biological processes such as cell growth and development or tissue repair.

170 168 Contents Chapter 7 7 Cytokines and Growth Factors 7.1 Cytokines, Chemokines, Growth Factors, and soluble Receptors CD Antigens

171 7 Cytokines and Growth Factors Cytokines, Chemokines, Growth Factors, and soluble Receptors n Human Product Name Product Description Size* Catalog Number 4-1BB sreceptor (CD137), human, recombinant Recombinant Human 4-1BB soluble Receptor (CD137) 20 µg C BBL (CD137L), human, recombinant Recombinant Human 4-1BB Ligand (CD137L) 20 µg C Acrp30 (His), human, recombinant Acrp30 (E. coli), human, recombinant Acrp30 (HEK), human, recombinant Acrp30 (Sf9), human, recombinant gacrp30 (Adipolean), human, recombinant Recombinant Human Adiponectin (Adipocyte Complement Related Protein of 30 kda; E. coli, His) Recombinant Human Adiponectin (Adipocyte Complement Related Protein of 30 kda; E. coli) Recombinant Human Adiponectin (Adipocyte Complement Related Protein of 30 kda; HEK) Recombinant Human Adiponectin (Adipocyte Complement Related Protein of 30 kda; Sf9) 50 µg C µg C µg C µg C Recombinant Human globular Acrp30 (Adipolean) 25 µg C Activin A, human, recombinant Recombinant Human Activin A 5 µg C Activin B, human, recombinant Recombinant Human Activin B 5 µg C AITRL, human, recombinant Recombinant Human Activation Induced TNFR Member Ligand 20 µg C Ang-1, human, recombinant (Sf9) Recombinant Human Angiopoietin 1 (Sf9) 10 µg C Ang-2, human, recombinant (Sf9) Recombinant Human Angiopoietin 2 (Sf9) 10 µg C ANGPTL-3, human, recombinant Recombinant Human Angiopoietin-like Protein 3 10 µg C ANGPTL-4, human, recombinant Recombinant Human Angiopoietin-like Protein 4 10 µg C APO-A1, human, recombinant Recombinant Human Apolipoprotein A1 100 µg C APO-E2, human, recombinant Recombinant Human Apolipoprotein E2 100 µg C APO-E3, human, recombinant Recombinant Human Apolipoprotein E3 100 µg C APO-E4, human, recombinant Recombinant Human Apolipoprotein E4 100 µg C Apo-SAA, human, recombinant Recombinant Human Apolipoprotein Serum Amyloid A 50 µg C Artemin, human, recombinant Recombinant Human Artemin 10 µg C BAFF, human, recombinant BAFF-R, human, recombinant BCA-1 (BLC, CXCL13), human, recombinant Recombinant Human B cell Activating Factor (BLyS) belonging to the TNF Family Recombinant Human B-cell Activating Factor (BLyS) Receptor belonging to the TNF Family Recombinant Human B-cell Attracting Chemokine 1 (BLC, CXCL13) 20 µg C µg C µg C BCMA, human, recombinant Recombinant Human B-cell Maturation Protein 20 µg C BD-1 (36 aa), human, recombinant Recombinant Human beta-defensin 1 (36 aa) 20 µg C BD-1 (47 aa), human, recombinant Recombinant Human beta-defensin 1 (47 aa) 20 µg C BD-2, human, recombinant Recombinant Human beta-defensin 2 20 µg C BD-3, human, recombinant Recombinant Human beta-defensin 3 20 µg C BD-4, human, recombinant Recombinant Human beta-defensin 4 20 µg C BDNF, human, recombinant Recombinant Human Brain Derived Neurotrophic Factor 10 µg C BMP-2, human, recombinant Recombinant Human Bone Morphogenetic Protein 2 10 µg C BMP-4, human, recombinant Recombinant Human Bone Morphogenetic Protein 4 10 µg C BMP-6, human, recombinant Recombinant Human Bone Morphogenetic Protein 6 10 µg C Cytokines and Growth Factors * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

172 170 7 Cytokines and Growth Factors Product Name Product Description Size* Catalog Number BMP-7, human, recombinant Recombinant Human Bone Morphogenetic Protein 7 10 µg C Cytokines and Growth Factors BMP-13 (CDMP-2), human, recombinant BMP-14 (CDMP-1), human, recombinant sbmpr-1a, human, recombinant (Sf9) Recombinant Human Bone Morphogenetic Protein 13 (CDMP-2) Recombinant Human Bone Morphogenetic Protein 14 (CDMP-1) Recombinant Human soluble Bone Morphogenetic Protein Receptor 1A (Sf9) 50 µg C µg C µg C BNP, human, recombinant Recombinant Human B-type Natriuretic Protein 10 µg C BRAK (CXCL14), human, recombinant Recombinant Human Human Breast and Kidney Chemokine (CXCL14) 20 µg C BTC, human, recombinant Recombinant Human Betacellulin 20 µg C Cardiotrophin-1 (CT-1), human, recombinant Recombinant Human Cardiotrophin-1 (CT-1) 10 µg C CD1A, human, recombinant Recombinant Human CD1A 2 µg C CD2, human, recombinant Recombinant Human CD2 2 µg C scd4, human, recombinant Recombinant Human soluble CD4 10 µg C scd4, human, recombinant Recombinant Human soluble CD4 50 µg C CD7, human, recombinant Recombinant Human CD7 2 µg C CD9, human, recombinant Recombinant Human CD9 2 µg C CD10, human, recombinant Recombinant Human CD10 2 µg C CD11b, human, recombinant Recombinant Human CD11b 2 µg C CD14, human, recombinant (E. coli) Recombinant Human CD14 (E. coli) 2 µg C CD14, human, recombinant (CHO) Recombinant Human CD14 (CHO) 2 µg C CD21, human, recombinant Recombinant Human CD21 2 µg C CD22, human, recombinant (CHO) Recombinant Human CD22 (CHO) 20 µg C CD23, human, recombinant Recombinant Human CD23 2 µg C CD29, human, recombinant Recombinant Human CD29 2 µg C CD31, human, recombinant Recombinant Human CD31 2 µg C CD34, human, recombinant Recombinant Human CD34 2 µg C CD38, human, recombinant Recombinant Human CD38 2 µg C scd40 Ligand (CD154), human, recombinant Recombinant Human soluble CD40 Ligand (TRAP, CD154) 50 µg C CD43, human, recombinant Recombinant Human CD43 2 µg C CD45, human, recombinant Recombinant Human CD45 2 µg C CD52, human, recombinant Recombinant Human CD52 2 µg C CD56, human, recombinant Recombinant Human CD56 2 µg C CD68, human, recombinant Recombinant Human CD68 2 µg C CD72, human, recombinant Recombinant Human CD72 2 µg C CD99, human, recombinant Recombinant Human CD99 2 µg C scd105 (Endoglin), human, recombinant (Sf9) Recombinant Human soluble CD105 (Endoglin; Sf9) 25 µg C CD146, human, recombinant Recombinant Human CD146 2 µg C * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

173 7 Cytokines and Growth Factors 171 Product Name Product Description Size* Catalog Number CD147, human, recombinant Recombinant Human CD147 2 µg C CD152 (CTLA-4), human, recombinant Recombinant Human CD152 (Cytotoxic T-lymphocyte-Associated Antigen 4) 50 µg C CNTF, human, recombinant Recombinant Human Ciliary Neurotrophic Factor 20 µg C CRP, human, recombinant Recombinant Human C-Reactive Protein 1 mg C CTACK (CCL27), human, recombinant CTGF (98 aa), human, recombinant CTGF ( aa, His), human, recombinant CTGF (His), human, recombinant CTGFL (WISP-2), human, recombinant Recombinant Human Cutaneous T-cell Attracting Chemokine (CCL27) Recombinant Human Connective Tissue Growth Factor (98 aa) Recombinant Human Connective Tissue Growth Factor ( aa, His) Recombinant Human Connective Tissue Growth Factor (His) Recombinant Human Connective Tissue Growth Factor Like Protein (WISP-2) 20 µg C µg C µg C µg C µg C CXCL16, human, recombinant Recombinant Human CXC Ligand µg C CYR61, human, recombinant Recombinant Human Cysteine-rich Angiogenic Inducer µg C sdll-4, human, recombinant Recombinant Human soluble Delta-like Protein 4 25 µg C EGF, human, recombinant (E. coli) Recombinant Human Epidermal Growth Factor (E. coli) 500 µg C EGF, human, recombinant (Pichia) Recombinant Human Epidermal Growth Factor (Pichia) 500 µg C segf-r, human, recombinant EG-VEGF, human, recombinant EMAP-II, human, recombinant ENA-78, human, recombinant Recombinant Human soluble Epidermal Growth Factor Receptor Recombinant Human Vascular Endothelial Cell Growth Factor-EG Recombinant Human Endothelial/Monocyte Activating Polypeptide II Recombinant Human Epithelial Neutrophil Activating Peptide µg C µg C µg C µg C Endostatin, human, recombinant Recombinant Human Endostatin 100 µg C Eotaxin (CCL11), human, recombinant Recombinant Human Eotaxin (CCL11) 20 µg C Eotaxin-2 (CCL24), human, recombinant Recombinant Human Eotaxin 2 (CCL24) 20 µg C Eotaxin-3 (CCL26), human, recombinant Recombinant Human Eotaxin 3 (CCL26) 20 µg C Epigen, human, recombinant Recombinant Human Epigen 25 µg C Epiregulin, human, recombinant Recombinant Human Epiregulin 25 µg C EPO-alpha, human, recombinant Recombinant Human Erythropoietin alpha 50 µg C EPO-beta, human, recombinant Recombinant Human Erythropoietin beta 500 IU C E-selectin, human, recombinant Recombinant Human E-selectin 10 µg C Exodus-2, human, recombinant Recombinant Human Exodus 2 20 µg C sfas Ligand, human, recombinant Recombinant Human soluble Fas Ligand 10 µg C sfas Receptor (CD95), human, recombinant FGF-a (FGF-1), human, recombinant (E. coli) FGF-a (FGF-1), human, recombinant (Sf9) Recombinant Human soluble Fas Receptor (CD95, TNFRSF6) Recombinant Human acidic Fibroblast Growth Factor (FGF-1; E. coli) Recombinant Human acidic Fibroblast Growth Factor (FGF-1; Sf9) 20 µg C µg C µg C Cytokines and Growth Factors * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

174 172 7 Cytokines and Growth Factors Product Name Product Description Size* Catalog Number 7 Cytokines and Growth Factors FGF-b (FGF-2), human, recombinant FGF-b (FGF-2, 147 aa), human, recombinant Recombinant Human basic Fibroblast Growth Factor (FGF-2) Recombinant Human basic Fibroblast Growth Factor (FGF-2, 147 aa) 50 µg C µg C FGF-4, human, recombinant Recombinant Human Fibroblast Growth Factor 4 25 µg C FGF-5, human, recombinant Recombinant Human Fibroblast Growth Factor 5 50 µg C FGF-6, human, recombinant Recombinant Human Fibroblast Growth Factor 6 25 µg C FGF-8, human, recombinant Recombinant Human Fibroblast Growth Factor 8 25 µg C FGF-9, human, recombinant (Sf9) Recombinant Human Fibroblast Growth Factor 9 (Sf9) 20 µg C FGF-10, human, recombinant Recombinant Human Fibroblast Growth Factor µg C FGF-16, human, recombinant Recombinant Human Fibroblast Growth Factor µg C FGF-17, human, recombinant Recombinant Human Fibroblast Growth Factor µg C FGF-18, human, recombinant Recombinant Human Fibroblast Growth Factor µg C FGF-19, human, recombinant Recombinant Human Fibroblast Growth Factor µg C FGF-20, human, recombinant Recombinant Human Fibroblast Growth Factor µg C FGF-21, human, recombinant Recombinant Human Fibroblast Growth Factor µg C FGF-22, human, recombinant Recombinant Human Fibroblast Growth Factor µg C sfgfr-1, human, recombinant (Sf9) sfgfr-2, human, recombinant (Sf9) sfgfr-3, human, recombinant (Sf9) sfgfr-4, human, recombinant (Sf9) Recombinant Human Fibroblast soluble Growth Factor Receptor 1a (IIIc) / Fc-Chimera (Sf9) Recombinant Human Fibroblast soluble Growth Factor Receptor 2a (IIIc) / Fc-Chimera (Sf9) Recombinant Human Fibroblast soluble Growth Factor Receptor 3a (IIIc) / Fc-Chimera (Sf9) Recombinant Human Fibroblast soluble Growth Factor Receptor 4 (IIIc) / Fc-Chimera (Sf9) 10 µg C µg C µg C µg C flt-3 Ligand, human, recombinant (E. coli) Recombinant Human flt3 Ligand (E. coli) 10 µg C flt-3 Ligand, human, recombinant (Sf9) Recombinant Human flt3 Ligand (Sf9) 10 µg C Fractalkine (CX3CL1), human, recombinant Recombinant Human Fractalkine (CX3CL1) 20 µg C sfrp-1, human, recombinant Recombinant Human Secreted Frizzled Related Protein 1 20 µg C FST, human, recombinant Recombinant Human Follistatin 20 µg C Galectin-1, human, recombinant Recombinant Human Galectin 1 50 µg C Galectin-2, human, recombinant Recombinant Human Galectin 2 50 µg C Galectin-3, human, recombinant Recombinant Human Galectin 3 50 µg C GCP-2 (CXCL6), human, recombinant G-CSF, human, recombinant (E. coli) G-CSF, human, recombinant (CHO) Recombinant Human Human Granulocyte Chemotactic Peptide 2 (CXCL6) Recombinant Human Granulocyte Colony Stimulating Factor (E. coli) Recombinant Human Granulocyte Colony Stimulating Factor (CHO) 20 µg C µg C µg C GDF-3, human, recombinant Recombinant Human Growth & Differentiation Factor 3 20 µg C GDF-5, human, recombinant Recombinant Human Growth & Differentiation Factor 5 10 µg C GDF-8, human, recombinant Recombinant Human Growth & Differentiation Factor 8 (Myostatin Propeptide) 25 µg C * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

175 7 Cytokines and Growth Factors 173 Product Name Product Description Size* Catalog Number GDF-9, human, recombinant Recombinant Human Growth & Differentiation Factor 9 20 µg C GDF-11, human, recombinant Recombinant Human Growth & Differentiation Factor µg C GDF-15 (MIC-1), human, recombinant Recombinant Human Growth & Differentiation Factor 15 (MIC-1) 20 µg C GDNF, human, recombinant Recombinant Human Glial Derived Neurotropic Factor 10 µg C GH-20K-pit, human, recombinant Recombinant Human Pituitary Growth Hormone 20K 100 µg C GH-20K-pl, human, recombinant Recombinant Human Placental Growth Hormone 20K 100 µg C GH-22K-pl, human, recombinant Recombinant Human Placental Growth Hormone 22K 100 µg C GHBP, human, recombinant Recombinant Human Growth Hormone Binding Protein 100 µg C GM-CSF, human, recombinant (E. coli) GM-CSF, human, recombinant (Sf9) GM-CSF/IL-3 FP, human, recombinant (Sf9) Recombinant Human Granulocyte/Macrophage Colony Stimulating Factor (E. coli) Recombinant Human Granulocyte/Macrophage Colony Stimulating Factor (Sf9) Recombinant Human Granulocyte/Macrophage Colony Stimulating Factor/IL-3 Fusion Protein (Sf9) 10 µg C µg C µg C GMF-beta, human, recombinant Recombinant Human Glia Maturation Factor beta 10 µg C GRO-alpha (MGSA, CXCL1), human, recombinant GRO-beta (MIP-2, CXCL2), human, recombinant GRO-gamma (CXCL3), human, recombinant Growth Hormone (GH), human, recombinant HB-EGF, human, recombinant HCC-1 (72 aa, CCL14), human, recombinant HCC-1 (66 aa, CCL14), human, recombinant Recombinant Human Growth Regulated Oncogene alpha (Melanoma Growth Stimulating Activity, CXCL1) Recombinant Human Growth Regulated Oncogene beta (CXCL2) Recombinant Human Growth Regulated Oncogene gamma (CXCL3) 25 µg C µg C µg C Recombinant Human Growth Hormone (GH) 100 µg C Recombinant Human Heparin Binding EGF-like Growth Factor Recombinant Human Hemofiltrate CC-Chemokine 1 (72 aa, CCL14) Recombinant Human Hemofiltrate CC-Chemokine 1 (66 aa, CCL14) 50 µg C µg C µg C HGF, human, recombinant Recombinant Human Hepatocyte Growth Factor 10 µg C HVEM/Fc, human, recombinant Recombinant Human Herpes Virus Entry Mediator (Fc Chimera) 100 µg C I-309 (CCL1), human, recombinant Recombinant Human I-309 (CCL1) 10 µg C IFN-alpha 1, human, recombinant Recombinant Human Interferon alpha-1 (E. coli) 100 µg C IFN-alpha 2a, human, recombinant Recombinant Human Interferon alpha-2a (E. coli) 100 µg C IFN-alpha 2b, human, recombinant (E. coli) IFN-alpha 2b, human, recombinant (Yeast) IFN-beta 1a, human, recombinant (CHO) Recombinant Human Interferon alpha-2b (E. coli) 100 µg C Recombinant Human Interferon alpha-2b (Yeast, glycosylated) 50 µg C Recombinant Human Interferon beta-1a (CHO) 10 µg C IFN-beta 1b, human, recombinant Recombinant Human Interferon beta-1b (E. coli) 10 µg C IFN-gamma, human, recombinant Recombinant Human Interferon gamma 100 µg C IFN-lambda-1 (IL-29), human, recombinant Recombinant Human Interferon lambda-1 (IL-29) 20 µg C Cytokines and Growth Factors * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

176 174 7 Cytokines and Growth Factors Product Name Product Description Size* Catalog Number IFN-lambda-2 (IL-28A), human, recombinant Recombinant Human Interferon lambda-2 (IL-28A) 20 µg C Cytokines and Growth Factors IGF-BP1, human, recombinant IGF-BP2, human, recombinant IGF-BP3, human, recombinant IGF-BP4, human, recombinant IGF-BP5, human, recombinant IGF-BP6, human, recombinant IGF-BP7, human, recombinant Recombinant Human Insulin Like Growth Factor Binding Protein 1 Recombinant Human Insulin Like Growth Factor Binding Protein 2 Recombinant Human Insulin Like Growth Factor Binding Protein 3 Recombinant Human Insulin Like Growth Factor Binding Protein 4 Recombinant Human Insulin Like Growth Factor Binding Protein 5 Recombinant Human Insulin Like Growth Factor Binding Protein 6 Recombinant Human Insulin Like Growth Factor Binding Protein 7 20 µg C µg C µg C µg C µg C µg C µg C IGF-I, human, recombinant Recombinant Human Insulin Like Growth Factor I 100 µg C IGF-II, human, recombinant Recombinant Human Insulin Like Growth Factor II 50 µg C IGF-IR, human, recombinant Recombinant Human Insulin-Like Growth Factor I Receptor 5 µg C IL-1 alpha, human, recombinant Recombinant Human Interleukin 1-alpha 10 µg C IL-1 beta, human, recombinant Recombinant Human Interleukin 1-beta 10 µg C IL-1 RA, human, recombinant Recombinant Human Interleukin 1 Receptor Antagonist 10 µg C IL-2, human, recombinant Recombinant Human Interleukin 2 50 µg C IL-2 CC, human, recombinant Recombinant Human Interleukin 2 (for Cell Culture) ~ 5 µg C sil-2r alpha (CD25), human, recombinant Recombinant Human soluble Interleukin 2 Receptor alpha (CD25) ~ 25 µg C IL-3, human, recombinant (E. coli) Recombinant Human Interleukin 3 (E. coli) 10 µg C IL-3, human, recombinant (E. coli) Recombinant Human Interleukin 3 (E. coli) 25 µg C IL-3, human, recombinant (Sf9) Recombinant Human Interleukin 3 (Sf9) 10 µg C IL-3 CC, human, recombinant Recombinant Human Interleukin 3 (for Cell Culture) ~ 30 µg C IL-4, human, recombinant (E. coli) Recombinant Human Interleukin 4 (E. coli) 10 µg C IL-4, human, recombinant (CHO) Recombinant Human Interleukin 4 (CHO) 10 µg C IL-4 CC, human, recombinant Recombinant Human Interleukin 4 for (for Cell Culture) ~ 5 µg C sil-4 Receptor (CD124), human, recombinant Recombinant Human Soluble Interleukin 4 Receptor alpha (CD124) 15 µg C IL-5, human, recombinant Recombinant Human Interleukin 5 10 µg C IL-6, human, recombinant (E. coli) Recombinant Human Interleukin 6 (E. coli) 20 µg C IL-6, human, recombinant Recombinant Human Interleukin 6 25 µg C IL-6 (CHO), human, recombinant Recombinant Human Interleukin 6 (CHO) 10 µg C sil-6 Receptor (CD126), human, recombinant Recombinant Human soluble Interleukin 6 Receptor (CD126) 20 µg C IL-7, human, recombinant (E. coli) Recombinant Human Interleukin 7 (E. coli) 10 µg C IL-7, human, recombinant (Yeast) Recombinant Human Interleukin 7 (Yeast) 10 µg C * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

177 7 Cytokines and Growth Factors 175 Product Name Product Description Size* Catalog Number IL-8 (CXCL8, 72 aa), human, recombinant Recombinant Human Interleukin 8 (CXCL8, 72 aa form) 25 µg C IL-8 (CXCL8, 77 aa), human, recombinant Recombinant Human Interleukin 8 (CXCL8, 77 aa form) 20 µg C IL-9, human, recombinant Recombinant Human Interleukin 9 10 µg C IL-10, human, recombinant (E. coli) Recombinant Human Interleukin 10 (E. coli) 10 µg C IL-10, human, recombinant (CHO) Recombinant Human Interleukin 10 (CHO) 10 µg C IL-11, human, recombinant Recombinant Human Interleukin µg C IL-12, human, recombinant Recombinant Human Interleukin µg C IL-12-p40, human, recombinant (CHO) Recombinant Human Interleukin 12-p40 (CHO) 10 µg C IL-12-p80, human, recombinant (CHO) Recombinant Human Interleukin 12-p80 (CHO) 10 µg C IL-13, human, recombinant Recombinant Human Interleukin µg C IL-13 (Variant), human, recombinant Recombinant Human Interleukin 13 (Variant) 10 µg C IL-15, human, recombinant Recombinant Human Interleukin µg C IL-16 (121 aa), human, recombinant Recombinant Human Interleukin 16 (121 aa) 10 µg C IL-16 (130 aa), human, recombinant Recombinant Human Interleukin 16 (130 aa) 10 µg C IL-17, human, recombinant Recombinant Human Interleukin 17A 25 µg C IL-17B, human, recombinant Recombinant Human Interleukin 17B 25 µg C IL-17D, human, recombinant Recombinant Human Interleukin 17D 25 µg C IL-17E, human, recombinant Recombinant Human Interleukin 17E 25 µg C IL-17F, human, recombinant Recombinant Human Interleukin 17F 25 µg C IL-19, human, recombinant Recombinant Human Interleukin µg C IL-20, human, recombinant Recombinant Human Interleukin µg C IL-21, human, recombinant Recombinant Human Interleukin µg C IL-22, human, recombinant Recombinant Human Interleukin µg C IL-24, human, recombinant Recombinant Human Interleukin µg C IL-31, human, recombinant Recombinant Human Interleukin µg C IL-32, human, recombinant Recombinant Human Interleukin µg IL-33, human, recombinant Recombinant Human Interleukin µg C Insulin, human, recombinant Recombinant Human Insulin 25 mg C IP-10 (CXCL10), human, recombinant Recombinant Human IFN Inducible Protein 10 (CXCL10) 25 µg C I-TAC (CXCL11), human, recombinant Recombinant Human Interferon-inducible T-cell alpha Chemoattractant (CXCL11) 20 µg C KGF (FGF-7), human, recombinant Recombinant Human Keratinocyte Growth Factor (FGF-7) 10 µg C KGF-2, human, recombinant Recombinant Human Keratinocyte Growth Factor 2 25 µg C LAG-1 (CCL4L1), human, recombinant Recombinant Human Lymphocyte Activation Gene 1 (CCL4L1) LD78 beta (CCL3L1), human, recombinant 20 µg C Recombinant Human LD78 beta (CCL3L1) 20 µg C LEC (NCC-4), human, recombinant Recombinant Human Liver-Expressed Chemokine (NCC-4) 20 µg C Leptin, human, recombinant Recombinant Human Leptin (Anti-Obesity Protein) 1 mg C LeukinFeron, human, native Human LeukinFeron 10,000 IU C Cytokines and Growth Factors * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

178 176 7 Cytokines and Growth Factors Product Name Product Description Size* Catalog Number 7 Cytokines and Growth Factors LFA-3, human, recombinant Recombinant Human Leukocyte Function Associated Antigen 3, Fusion Protein 100 µg C LIGHT, human, recombinant Recombinant Human LIGHT 15 µg C L-selectin, human, recombinant Recombinant Human L-selectin 10 µg C Lymphotactin, human, recombinant Recombinant Human Lymphotactin 10 µg C slyve-1, human, recombinant (Sf9) Recombinant Human Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (Sf9) 25 µg C Maspin, human, recombinant Recombinant Human Maspin 20 µg C MCP-1 (MCAF, CCL2), human, recombinant Recombinant Human Monocyte Chemotactic Protein 1 (MCAF, CCL2) MCP-2 (CCL8), human, recombinant Recombinant Human Monocyte Chemotactic Protein 2 (CCL8) MCP-3 (CCL7), human, recombinant Recombinant Human Monocyte Chemotactic Protein 3 (CCL7) MCP-4 (CCL13), human, recombinant Recombinant Human Monocyte Chemotactic Protein 4 (CCL13) M-CSF, human, recombinant MDC (CCL22, 67 aa), human, recombinant MDC (CCL22, 69 aa), human, recombinant MEC (CCL28), human, recombinant MIA, human, recombinant MIA-2, human, recombinant Recombinant Human Macrophage Colony Stimulating Factor Recombinant Human Macrophage Derived Chemokine (67 aa) Recombinant Human Macrophage Derived Chemokine (69 aa) Recombinant Human Mucosae-Associated Epithelial Chemokine (CCL28) Recombinant Human Melanoma Inhibitory Activity Protein Recombinant Human Melanoma Inhibitory Activity Protein 2 20 µg C µg C µg C µg C µg C µg C µg C µg C µg C µg C Midkine, human, recombinant Recombinant Human Midkine 20 µg C MIF, human, recombinant Recombinant Human Mesoderm Inducing Factor 25 µg C MIG (CXCL9), human, recombinant MIP-1 alpha (CCL3), human, recombinant MIP-1 beta (CCL4), human, recombinant MIP-3 (CCL23), human, recombinant MIP-3 alpha (CCL20), human, recombinant MIP-3 beta (CCL19), human, recombinant MIP-4 (CCL18), human, recombinant MIP-5 (CCL15), human, recombinant Recombinant Human Monokine Induced by Interferon gamma (CXCL9) Recombinant Human Macrophage Inflammatory Protein 1-alpha (CCL3) Recombinant Human Macrophage Inflammatory Protein 1-beta (CCL4) Recombinant Human Macrophage Inflammatory Protein 3 (MPIF-1, CCL23) Recombinant Human Macrophage Inflammatory Protein 3-alpha (CCL20) Recombinant Human Macrophage Inflammatory Protein 3-beta (CCL19) Recombinant Human Macrophage Inflammatory Protein 4 (CCL18) Recombinant Human Macrophage Inflammatory Protein 5 (CCL15) 20 µg C µg C µg C µg C µg C µg C µg C µg C MMP-3, human, recombinant Recombinant Human Matrix Metalloproteinase 3 20 µg C * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

179 7 Cytokines and Growth Factors 177 Product Name Product Description Size* Catalog Number MMP-7, human, recombinant Recombinant Human Matrix Metalloproteinase 7 20 µg C Myostatin, human, recombinant Recombinant Human Myostatin 10 µg C Nanog, human, recombinant Recombinant Human Nanog 20 µg C NAP-2 (CXCL7), human, recombinant Recombinant Human Neutrophil Activating Protein 2 (CXCL7) 10 µg C Neuregulin-1 (NRG-1), human, recombinant Recombinant Human Neuregulin 1 (NRG-1, Heregulin beta-2) 50 µg C Neuroglobin, human, recombinant Recombinant Human Neuroglobin 10 µg C Neuroserpin, human, recombinant Recombinant Human Neuroserpin 25 µg C Neurturin (NTN), human, recombinant Recombinant Human Neurturin (NTN) 20 µg C beta-ngf, human, recombinant (CHO) Recombinant Human Nerve Growth Factor beta (CHO) 20 µg C NNT-1 (BCSF-3), human, recombinant Recombinant Human Novel Neurotrophin 1 (BCSF-3) 10 µg C NOGGIN, human, recombinant Recombinant Human NOGGIN (dimer) 20 µg C NOV, human, recombinant Recombinant Human Nephroblastoma Overexpressed Gene NP-1, human, recombinant Recombinant Human Neutrophil Peptide 1 (alpha-defensin-1) 20 µg C µg C NT-3, human, recombinant Recombinant Human Neurotrophin-3 10 µg C NT-4, human, recombinant Recombinant Human Neurotrophin-4 10 µg C Omentin, human, recombinant Recombinant Human Omentin 10 µg C Oncostatin M, human, recombinant Recombinant Human Oncostatin M (209 aa) 10 µg C OPG (OCIF), human, recombinant Recombinant Human Osteoprotegerin (Osteoclastogenesis Inhibitory Factor) 50 µg C OTOR, human, recombinant Recombinant Human Otoraplin 20 µg C OX40L, human, recombinant Recombinant Human OX40 Ligand 10 µg C PAF-AH, human, recombinant Recombinant Human Platelet Activating Factor (Acetylhydrolase) 10 µg C PAI-2, human, recombinant Recombinant Human Plasminogen Activator Inhibitor 2 10 µg C PDGF-AA, human, recombinant Recombinant Human Platelet Derived Growth Factor AA 10 µg C PDGF-AB, human, recombinant Recombinant Human Platelet Derived Growth Factor AB 10 µg C PDGF-BB, human, recombinant (E. coli) PDGF-BB, human, recombinant (Yeast) Recombinant Human Platelet Derived Growth Factor BB (E. coli) Recombinant Human Platelet Derived Growth Factor BB (Yeast) 10 µg C µg C PDGF-CC, human, recombinant Recombinant Human Platelet Derived Growth Factor CC 20 µg C PEDF, human, recombinant Recombinant Human Pigment Epithelium Derived Factor 20 µg C Periostin, human, recombinant Recombinant Human Periostin 10 µg C Persephin, human, recombinant Recombinant Human Persephin 20 µg C PF-4 (CXCL4), human, recombinant Recombinant Human Platelet Factor 4 (CXCL4) 20 µg C Pleiotrophin, human, recombinant Recombinant Human Pleiotrophin 20 µg C PLGF-1, human, recombinant (Sf9) Recombinant Human Placenta Growth Factor 1 (Sf9) 10 µg C PLGF-1, human, recombinant (E. coli) Recombinant Human Placenta Growth Factor 1 (E. coli-derived) 25 µg C Cytokines and Growth Factors * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

180 178 7 Cytokines and Growth Factors Product Name Product Description Size* Catalog Number PLGF-2, human, recombinant (Sf9) Recombinant Human Placenta Growth Factor-2 (Sf9) 10 µg C PLGF-2, human, recombinant (E. coli) Recombinant Human Placenta Growth Factor-2 (E. coli) 10 µg C ProBMP-2, human, recombinant Recombinant Human Pro Bone Morphogenetic Protein 2 10 µg C PGRN, human, recombinant Recombinant Human Progranulin 10 µg C Prokinectin-2, human, recombinant Recombinant Human Prokinectin 2 20 µg C Prolactin, human, recombinant Recombinant Human Prolactin 50 µg C Prolactin Receptor (Prlr), human, recombinant Recombinant Human Prolactin Receptor (Prlr) 5 µg C Pro-NGF, human, recombinant Recombinant Human Pro-Nerve Growth Factor 10 µg C Prox-1 (fragment), human, recombinant Recombinant Human Prox-1 (fragment) 5 µg C P-selectin, human, recombinant Recombinant Human P-selectin 10 µg C Cytokines and Growth Factors PTHrP, human, recombinant srank Receptor, human, recombinant srankl, human, recombinant Recombinant Human Parathyroid Hormone-like Related Protein Recombinant Human soluble Receptor Activator of NF-Kappa-B Receptor (TNFRSF11A) Recombinant Human soluble Receptor Activator of NF-Kappa-B Ligand 50 µg C µg C µg C RANTES (CCL5), human, recombinant Recombinant Human RANTES (CCL5) 20 µg C Relaxin-2, human, recombinant Recombinant Human Relaxin 2 25 µg C Relaxin-3, human, recombinant Recombinant Human Relaxin 3 25 µg C RELM beta, human, recombinant Recombinant Human Resistin-like Molecule beta 25 µg C Resistin, human, recombinant Recombinant Human Resistin 25 µg C SCF, human, recombinant (E. coli) Recombinant Human Stem Cell Factor (E. coli) 10 µg C SCF, human, recombinant (Sf9) Recombinant Human Stem Cell Factor (Sf9) 10 µg C SCGF-alpha, human, recombinant Recombinant Human Stem Cell Growth Factor alpha 10 µg C SCGF-beta, human, recombinant Recombinant Human Stem Cell Growth Factor beta 10 µg C SDF-1 alpha (CXCL12), human, recombinant SDF-1 beta (CXCL12), human, recombinant Recombinant Human Stromal Cell Derived Factor 1-alpha (CXCL12) Recombinant Human Stromal Cell Derived Factor 1-beta (CXCL12) 10 µg C µg C Shh, human, recombinant (InC) Recombinant Human Sonic Hedge Hog (InC) 10 µg C TACI, human, recombinant Recombinant Human Transmembrane Activator and CAML Interactor 20 µg C TAFA-2, human, recombinant Recombinant Human TAFA 2 10 µg C TARC (CCL17), human, recombinant TECK (CCL25), human, recombinant Recombinant Human Thymus and Activation Regulated Chemokine (CCL17) Recombinant Human Thymus Expressed Chemokine (CCL25) 20 µg C µg C TFF-1, human, recombinant Recombinant Human Trefoil Factor 1 20 µg C TFF-2, human, recombinant Recombinant Human Trefoil Factor 2 20 µg C TGF-alpha, human, recombinant Recombinant Human Transforming Growth Factor alpha 100 µg C TGF-beta 1, human, recombinant (CHO) Recombinant Human Transforming Growth Factor beta-1 (CHO) 5 µg C * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

181 7 Cytokines and Growth Factors 179 Product Name Product Description Size* Catalog Number TGF-beta 1, human, recombinant (A293 cells) TGF-beta 2, human, recombinant (A293 cells) TGF-beta 2, human, recombinant (Sf9) Recombinant Human Transforming Growth Factor beta-1 (mammalian A293 cell-derived) Recombinant Human Transforming Growth Factor beta-2 (mammalian A293 cell-derived) Recombinant Human Transforming Growth Factor beta-2 (Sf9) 5 µg C µg C µg C TGF-beta 3, human, recombinant Recombinant Human Transforming Growth Factor beta-3 10 µg C Thrombin, human, recombinant Recombinant Human Thrombin 500 µg C stie-1/fc Chimera, human, recombinant (Sf9) stie-2/fc Chimera, human, recombinant (Sf9) Recombinant Human soluble TIE-1/Fc Chimera (Sf9) 20 µg C Recombinant Human soluble TIE-2/Fc Chimera (Sf9) 20 µg C TIMP-1, human, recombinant Recombinant Human Tissue Inhibitor of Metalloproteinases 10 µg C TL-1A, human, recombinant Recombinant Human TNF-like 1A (TNFSF15) 20 µg C TLR-3 (CD283), human, recombinant Recombinant Human Toll-like receptor 3 (CD283) 25 µg C TNF-alpha, human, recombinant (E. coli) TNF-alpha, human, recombinant (E. coli) Recombinant Human Tumor Necrosis Factor alpha (E. coli) Recombinant Human Tumor Necrosis Factor alpha (E. coli) 50 µg C µg C TNF-alpha, human, recombinant (Yeast) Recombinant Human Tumor Necrosis Factor alpha (Yeast) 10 µg C TNF-alpha, human, recombinant (Yeast) Recombinant Human Tumor Necrosis Factor alpha (Yeast) 50 µg C TNF-alpha M (151 aa), human, recombinant Recombinant Human Tumor Necrosis Factor alpha, Mutant/Variant (151 aa) 50 µg C TNF-beta, human, recombinant Recombinant Human Tumor Necrosis Factor beta 20 µg C TNF-beta, human, recombinant (InC) Recombinant Human Tumor Necrosis Factor beta (InC) 20 µg C stnf-r1 (CD120a), human, recombinant stnf-r2 (CD120b), human, recombinant TNF-Receptor Fusion Protein, human, recombinant stnf-receptor Inhibitor, human, recombinant TPO, human, recombinant (E. coli) TPO, human, recombinant (CHO) TRAIL (Apo2L), human, recombinant strail Receptor-1 (DR4), human, recombinant strail Receptor-2 (DR5), human, recombinant Recombinant Human soluble Tumor Necrosis Factor Receptor Type I (CD120a) Recombinant Human soluble Tumor Necrosis Factor Receptor Type II (CD120b) Recombinant Human Tumor Necrosis Factor Receptor Fusion Protein Recombinant Human soluble Tumor Necrosis Factor Receptor Inhibitor Recombinant Human Thrombopoietin (Mp1-ligand, MGDF; E. coli) Recombinant Human Thrombopoietin (Mp1-ligand, MGDF, CHO) Recombinant Human Tumor Necrosis Factor-Related Apoptosis Inducing Ligand Recombinant Human soluble TNF Related Apoptosis Inducing Ligand Receptor 1 (DR4) Recombinant Human soluble TNF Related Apoptosis Inducing Ligand Receptor 2 (DR5) 20 µg C µg C µg C µg C µg C µg C µg C µg C µg C TSG, human, recombinant Recombinant Human Twisted Gastrulation Protein 50 µg C TSLP, human, recombinant Recombinant Human Thymic Stromal Lymphopoietin 10 µg C TWEAK, human, recombinant Recombinant Human TNF-Related Weak Inducer of Apoptosis 25 µg C Cytokines and Growth Factors * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

182 180 7 Cytokines and Growth Factors Product Name Product Description Size* Catalog Number 7 Cytokines and Growth Factors TWEAK Receptor, human, recombinant Recombinant Human TNF-Related Weak Inducer of Apoptosis Receptor 25 µg C Vaspin, human, recombinant Recombinant Human Vaspin 25 µg C VEGF-121, human, recombinant VEGF-121, human, recombinant (Sf9) VEGF-145, human, recombinant (Sf9) VEGF-165, human, recombinant (E. coli) VEGF-165, human, recombinant (Sf9) VEGF-165, human, recombinant (CHO) VEGF-189, human, recombinant VEGF-C, human, recombinant VEGF-E, Orv virus, recombinant VEGF-I, human, recombinant svegfr-1 (flt-1, native), human, recombinant (Sf9) svegfr-1 (flt-1, native), human, recombinant (Sf9) svegfr-1 (flt-1, D1-3), human, recombinant (Sf9) svegfr-1 (flt-1, D1-4), human, recombinant (Sf9) svegfr-1 (flt-1, D1-5), human, recombinant (Sf9) svegfr-1 (flt-1, D7-Fc), human, recombinant (Sf9) svegfr-2 (skdr, D7), human, recombinant (Sf9) svegfr-2 (skdr-fc, D7-Fc), human, recombinant (Sf9) svegfr-3 (flt-4, native) human, recombinant (Sf9) Recombinant Human Vascular Endothelial Cell Growth Factor 121 Recombinant Human Vascular Endothelial Cell Growth Factor 121 (Sf9) Recombinant Human Vascular Endothelial Cell Growth Factor 145 (Sf9-insect cell-derived) Recombinant Human Vascular Endothelial Cell Growth Factor 165 (E. coli) Recombinant Human Vascular Endothelial Cell Growth Factor 165 (Sf9) Recombinant Human Vascular Endothelial Cell Growth Factor 165 (CHO) Recombinant Human Vascular Endothelial Cell Growth Factor 189 Recombinant Human Vascular Endothelial Cell Growth Factor C Recombinant Orv Virus Vascular Endothelial Cell Growth Factor E Recombinant Human Vascular Endothelial Cell Growth Factor Inhibitor Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 1, (flt-1, native; Sf9) Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 1 (flt-1, native; Sf9) Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 1, Domain 1-3 (flt-1; Sf9) Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 1, Domain 1-4 (flt-1; Sf9) Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 1, Domain 1-5 (flt-1; Sf9) Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 1, Domain 1-7-Fc chimera (flt-1; Sf9) Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 2, Domain 1-7 (skdr; Sf9) Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 2, Domain 1-7-Fc chimera (skdr-fc; Sf9) Recombinant Human soluble Vascular Endothelial Growth Factor Receptor 3 (flt-4, native; Sf9) 5 µg C µg C µg C µg C µg C µg C µg C µg C µg C µg C µg C µg C µg C µg C µg C µg C µg C µg C µg C Visfatin, human, recombinant Recombinant Human Visfatin 20 µg C WISP-1, human, recombinant WISP-3, human, recombinant Recombinant Human WNT-1-Inducible Signaling Pathway Protein 1 Recombinant Human WNT-1-Inducible Signaling Pathway Protein 3 20 µg C µg C WNT-1, human, recombinant Recombinant Human WNT-1 10 µg C * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

183 7 Cytokines and Growth Factors 181 n Mouse Product Name Product Description Size* Catalog Number Acrp30, mouse, recombinant (HEK) Acrp30, mouse, recombinant (E. coli) gacrp30 (Adipolean), mouse, recombinant APRIL, mouse, recombinant BCA-1 (BLC, CXCL13), mouse, recombinant Recombinant Mouse Adiponectin (Adipocyte Complement Related Protein of 30 kda, glycosylated; HEK) Recombinant Mouse Adiponectin (Adipocyte Complement Related Protein of 30 kda; E. coli) 10 µg D µg D Recombinant Mouse globular Acrp30 (Adipolean) 10 µg D Recombinant Mouse APRIL (A Proliferation Inducing Ligand, TALL-2, TRDL-1a, TNFSF13) Recombinant Mouse Human B-cell Attracting Chemokine 1 (CXCL13) 20 µg D µg D beta-ngf, mouse, native Mouse Nerve Growth Factor beta 20 µg D C-10 (CCL6), mouse, recombinant Recombinant Mouse C-10 (CCL6) 10 µg D Cardiotrophin-1 (CT-1), mouse, recombinant Recombinant Mouse Cardiotrophin-1 (CT-1) 10 µg D CD14, mouse, recombinant (CHO) Recombinant Mouse CD14 (CHO) 2 µg D scd40 Ligand, mouse, recombinant scd105 (Endoglin), mouse, recombinant (Sf9) CTACK (CCL27), mouse, recombinant Recombinant Mouse soluble CD40 Ligand (TNFSF5, TRAP, CD154, gp39) 25 µg D Recombinant Mouse soluble CD105 (Endoglin, Sf9) 25 µg D Recombinant Mouse Cuteaneous T-cell Attracting Chemokine (CCL27) 20 µg D CXCL16, mouse, recombinant Recombinant Mouse CXC Ligand16 25 µg D EGF, mouse, recombinant Recombinant Mouse Epidermal Growth Factor 500 µg D Eotaxin (CCL11), mouse, recombinant Recombinant Mouse Eotaxin (CCL11) 10 µg D Eotaxin-2 (CCL24), mouse, recombinant Recombinant Mouse Eotaxin 2 (CCL24) 20 µg D Exodus-2 (CCL21), mouse, recombinant Recombinant Mouse Exodus-2 (CCL21) 20 µg D FGF-a (FGF-1), mouse, recombinant FGF-b (FGF-2), mouse, recombinant Recombinant Mouse acidic Fibroblast Growth Factor (FGF-1) Recombinant Mouse basic Fibroblast Growth Factor (FGF-2) 50 µg D µg D FGF-9, mouse, recombinant Recombinant Mouse Fibroblast Growth Factor 9 10 µg D flt-3 Ligand, mouse, recombinant Recombinant Mouse flt3 Ligand 10 µg D G-CSF, mouse, recombinant Recombinant Mouse Granulocyte Colony Stimulating Factor 10 µg D rhu GDNF, mouse, recombinant Recombinant Mouse Glial Derived Neurotropic Factor 10 µg D GM-CSF, mouse, recombinant Recombinant Mouse Granulocyte/Macrophage Colony Stimulating Factor 10 µg D Granzyme B, mouse, recombinant Recombinant Mouse Granzyme B 10 µg D IFN-gamma, mouse, recombinant Recombinant Mouse Interferon gamma 100 µg D IFN-lambda-2 (IL28A), mouse, recombinant Recombinant Mouse Interferon lambda-2 (IL-28A) 20 µg D IGF-I, mouse, recombinant Recombinant Mouse Insulin Like Growth Factor I 50 µg D IL-1 alpha, mouse, recombinant Recombinant Mouse Interleukin 1-alpha 10 µg D IL-1 beta, mouse, recombinant Recombinant Mouse Interleukin 1-beta 10 µg D IL-2, mouse, recombinant Recombinant Mouse Interleukin 2 20 µg D Cytokines and Growth Factors * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

184 182 7 Cytokines and Growth Factors Product Name Product Description Size* Catalog Number IL-3, mouse, recombinant Recombinant Mouse Interleukin 3 10 µg D IL-4, mouse, recombinant Recombinant Mouse Interleukin 4 10 µg D IL-5, mouse, recombinant Recombinant Mouse Interleukin 5 10 µg D IL-6, mouse, recombinant Recombinant Mouse Interleukin 6 10 µg D IL-7, mouse, recombinant Recombinant Mouse Interleukin 7 10 µg D IL-9, mouse, recombinant Recombinant Mouse Interleukin 9 10 µg D IL-10, mouse, recombinant Recombinant Mouse Interleukin µg D IL-11, mouse, recombinant Recombinant Mouse Interleukin µg D IL-12, mouse, recombinant (CHO) Recombinant Mouse Interleukin µg D IL-12, mouse, recombinant (InC) Recombinant Mouse Interleukin 12 (InC) 10 µg D IL-12-p40, mouse, recombinant (CHO) Recombinant Mouse Interleukin 12 p40 (CHO) 10 µg D IL-12-p80, mouse, recombinant (CHO) Recombinant Mouse Interleukin 12 p80 (CHO) 10 µg D IL-13, mouse, recombinant Recombinant Mouse Interleukin µg D IL-15, mouse, recombinant Recombinant Mouse Interleukin µg D IL-16, mouse, recombinant Recombinant Mouse Interleukin µg D IL-17, mouse, recombinant Recombinant Mouse Interleukin 17A 25 µg D IL-17F, mouse, recombinant Recombinant Mouse Interleukin 17F 25 µg D IL-20, mouse, recombinant Recombinant Mouse Interleukin µg D IL-21, mouse, recombinant Recombinant Mouse Interleukin µg D IL-22, mouse, recombinant Recombinant Mouse Interleukin µg D IL-25 (SF20), mouse, recombinant Recombinant Mouse Interleukin 25 (SF20) 10 µg D IL-27 (p28), mouse, recombinant Recombinant Mouse Interleukin 27 (p28) 10 µg D IL-31, mouse, recombinant Recombinant Mouse Interleukin µg D IL-33, mouse, recombinant Recombinant Mouse Interleukin µg D IP-10 (CXCL10), mouse, recombinant Recombinant Mouse IFN Inducible Protein 10 (CXCL10) 25 µg D Cytokines and Growth Factors I-TAC (CXCL11), mouse, recombinant MCP-1 (JE, CCL2), mouse, recombinant KC (GRO-alpha, CXCL1), mouse, recombinant Recombinant Mouse Interferon-inducible T-cell alpha Chemoattractant (CXCL11) Recombinant Mouse JE (Monocyte Chemotactic Protein, CCL2) Recombinant Mouse Keratinocyte Chemoattractant (GRO-alpha, CXCL1) 20 µg D µg D µg D Leptin, mouse, recombinant Recombinant Mouse Leptin 1 mg D LIGHT, mouse, recombinant Recombinant Mouse LIGHT 20 µg D LIX (CXCL5, 70 aa), mouse, recombinant LIX (CXCL5, 93 aa), mouse, recombinant Lungkine (CXCL15), mouse, recombinant slyve-1, mouse, recombinant (Sf9) Recombinant Mouse Lipopolysaccharide Induced CXC Chemokine (CXCL5, 70 aa) Recombinant Mouse Lipopolysaccharide Induced CXC Chemokine (CXCL5, 93 aa) 20 µg D µg D Recombinant Mouse Lungkine (CXCL15) 20 µg D Recombinant Mouse soluble Lymphatic Vessel Endothelial Hyaluronan Receptor 1 (Sf9) MCP-2 (CCL8), mouse, recombinant Recombinant Mouse Monocyte Chemotactic Protein 2 (CCL8) 25 µg D µg D * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

185 7 Cytokines and Growth Factors 183 Product Name Product Description Size* Catalog Number MCP-3 (CCL7), mouse, recombinant Recombinant Mouse Monocyte Chemotactic Protein 3 (CCL7) MCP-5 (CCL12), mouse, recombinant Recombinant Mouse Monocyte Chemotactic Protein 5 (CCL12) 10 µg D µg D M-CSF, mouse, recombinant MDC (CCL22), mouse, recombinant MEC (CCL28), mouse, recombinant MIG (CXCL9), mouse, recombinant MIP-1 alpha (CCL3), mouse, recombinant MIP-1 beta (CCL4), mouse, recombinant MIP-1 gamma (CCL9/10), mouse, recombinant MIP-2 (GRO-beta, CXCL2), mouse, recombinant MIP-3 alpha (CCL20), mouse, recombinant MIP-3 beta (CCL19), mouse, recombinant Recombinant Mouse Macrophage Colony Stimulating Factor Recombinant Mouse Macrophage Derived Chemokine (CCL22) Recombinant Mouse Mucosae-Associated Epithelial Chemokine (CCL28) Recombinant Mouse Monokine Induced by Interferon-gamma (CXCL9) Recombinant Mouse Macrophage Inflammatory Protein 1-alpha (CCL3) Recombinant Mouse Macrophage Inflammatory Protein 1-beta (CCL4) Recombinant Mouse Macrophage Inflammatory Protein 1-gamma Recombinant Mouse Macrophage Inflammatory Protein 2 (CXCL2) Recombinant Mouse Macrophage Inflammatory Protein 3-alpha (CCL20) Recombinant Mouse Macrophage Inflammatory Protein 3-beta (CCL19) 10 µg D µg D µg D µg D µg D µg D µg D µg D µg D µg D Neuropoietin (NP), mouse, recombinant Recombinant Mouse Neuropoietin (NP, NPO) 25 µg D NOGGIN, mouse, recombinant Recombinant Mouse NOGGIN 20 µg D PDGF-BB, mouse, recombinant Recombinant Mouse Platelet Derived Growth Factor BB 10 µg D Persephin, mouse, recombinant Recombinant Mouse Persephin 20 µg D PLGF-2, mouse, recombinant Recombinant Mouse Placenta Growth Factor 2 10 µg D Prolactin, mouse, recombinant Recombinant Mouse Prolactin 250 µg D srankl, mouse, recombinant Recombinant Mouse soluble Receptor Activator of NF- Kappa B 10 µg D RANTES (CCL5), mouse, recombinant Recombinant Mouse RANTES (CCL5) 20 µg D RELM-alpha, mouse, recombinant Recombinant Mouse Resistin-like Molecule alpha 25 µg D RELM-beta, mouse, recombinant Recombinant Mouse Resistin-like Molecule beta 25 µg D RELM-gamma, mouse, recombinant Recombinant Mouse Resistin-like Molecule gamma 25 µg D Resistin, mouse, recombinant Recombinant Mouse Resistin 25 µg D SCF, mouse, recombinant Recombinant Mouse Stem Cell Factor 10 µg D SDF-1 alpha (CXCL12), mouse, recombinant SDF-1 beta (CXCL12), mouse, recombinant stie-1/fc Chimera, mouse, recombinant (Sf9) stie-2/fc Chimera, mouse, recombinant (Sf9) Recombinant Mouse Stromal Cell-Derived Factor 1-beta (CXCL12) Recombinant Mouse Stromal Cell-Derived Factor 1-alpha (CXCL12) 10 µg D µg D Recombinant Mouse soluble TIE-1/Fc Chimera (Sf9) 20 µg D Recombinant Mouse soluble TIE-2/Fc Chimera (Sf9) 20 µg D Cytokines and Growth Factors * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

186 184 7 Cytokines and Growth Factors Product Name Product Description Size* Catalog Number TNF-alpha, mouse, recombinant (E. coli) Recombinant Mouse Tumor Necrosis Factor alpha (E. coli) 20 µg D TNF-alpha, mouse, recombinant (S. lividans) Recombinant Mouse Tumor Necrosis Factor alpha (S. lividans) 20 µg D TPO, mouse, recombinant Recombinant Mouse Thrombopoietin 10 µg D TRAIL (Apo2L), mouse, recombinant VEGF-121, mouse, recombinant VEGF-164, mouse, recombinant VEGF-165, mouse, recombinant (E. coli) VEGF-165, mouse, recombinant (Sf9) Recombinant Mouse Tumor Necrosis Factor-Related Apoptosis Inducing Ligand Recombinant Murine Vascular Endothelial Cell Growth Factor 121 Recombinant Mouse Vascular Endothelial Growth Factor-164 Recombinant Mouse Vascular Endothelial Growth Factor-165 (E. coli) Recombinant Mouse Vascular Endothelial Growth Factor-165 (Sf9) 50 µg D µg D µg D µg D µg D Visfatin, mouse, recombinant Recombinant Mouse Visfatin 25 µg D n Rat Product Name Product Description Size Catalog Number Apolipoprotein-J, rat, recombinant Recombinant Rat Apolipoprotein J 10 µg E CNTF, rat, recombinant Recombinant Rat Ciliary Neurotrophic Factor 25 µg E EGF, rat, recombinant Recombinant Rat Epidermal Growth Factor 100 µg E FGF-b (FGF-2), rat, recombinant Recombinant Rat basic Fibroblast Growth Factor (FGF-2) 10 µg E FGF-9, rat, recombinant Recombinant Rat Fibroblast Growth Factor 9 10 µg E Fractalkine (CX3CL1), rat, recombinant Recombinant Rat Fractalkine (CX3CL1) 20 µg E GDNF, rat, recombinant Recombinant Rat Glial Derived Neurotropic Factor 10 µg E Cytokines and Growth Factors GM-CSF, rat, recombinant GRO-alpha (KC, CXCL1), rat, recombinant GRO-beta (MIP-2, CXCL2), rat, recombinant Growth Hormone (GH), rat, recombinant Recombinant Rat Granulocyte/Macrophage Colony Stimulating Factor Recombinant Rat Growth Regulated Oncogene alpha (KC, CXCL1) Recombinant Rat Growth Regulated Oncogene beta (MIP-2, CXCL2) 10 µg E µg E µg E Recombinant Rat Growth Hormone (GH) 500 µg E IFN-gamma, rat, recombinant Recombinant Rat Interferon gamma 100 µg E IGF-I, rat, recombinant Recombinant Rat Insulin Like Growth Factor I 20 µg E IL-1 alpha, rat, recombinant Recombinant Rat Interleukin 1-alpha 10 µg E IL-1 beta, rat, recombinant Recombinant Rat Interleukin 1-beta 10 µg E IL-2, rat, recombinant Recombinant Rat Interleukin 2 20 µg E IL-3, rat, recombinant Recombinant Rat Interleukin 3 10 µg E IL-3 beta, rat, recombinant Recombinant Rat Interleukin 3-beta 20 µg E IL-4, rat, recombinant Recombinant Rat Interleukin 4 10 µg E * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

187 7 Cytokines and Growth Factors 185 Product Name Product Description Size* Catalog Number IL-5, rat, recombinant Recombinant Rat Interleukin 5 10 µg E IL-6, rat, recombinant Recombinant Rat Interleukin 6 10 µg E IL-7, rat, recombinant Recombinant Rat Interleukin 7 10 µg E IL-10, rat, recombinant Recombinant Rat Interleukin µg E IL-12, rat, recombinant Recombinant Rat Interleukin µg E IL-13, rat, recombinant Recombinant Rat Interleukin µg E IL-13 beta, rat, recombinant Recombinant Rat Interleukin 13-beta 10 µg E IL-15, rat, recombinant Recombinant Rat Interleukin µg E IL-17, rat, recombinant Recombinant Rat Interleukin 17A 25 µg E IL-17E, rat, recombinant Recombinant Rat Interleukin 17E 10 µg E IL-18, rat, recombinant Recombinant Rat Interleukin µg E IP-10 (CXCL10), rat, recombinant Recombinant Rat IFN Inducible Protein 10 (CXCL10) 25 µg E Leptin, rat, recombinant Recombinant Rat Leptin 1 mg E MCP-1 (CCL2), rat, recombinant Recombinant Rat Monocyte Chemoattractant Protein 1 (CCL2) 10 µg E M-CSF, rat, recombinant Recombinant Rat Macrophage Colony Stimulating Factor 10 µg E MIP-1 alpha (CCL3), rat, recombinant MIP-1 beta (CCL4), rat, recombinant Recombinant Rat Macrophage Inflammatory Protein 1-alpha (CCL3) Recombinant Rat Macrophage Inflammatory Protein 1-beta (CCL4) 20 µg E µg E Prolactin, rat, recombinant Recombinant Rat Prolactin 250 µg E srankl, rat, recombinant Recombinant Rat soluble Receptor Activator of NF- Kappa-B Ligand 10 µg E RANTES (CCL5), rat, recombinant Recombinant Rat RANTES (CCL5) 20 µg E RAP, rat, recombinant Recombinant Rat Receptor Associated Protein 20 µg E Resistin, rat, recombinant Recombinant Rat Resistin 10 µg E SCF, rat, recombinant Recombinant Rat Stem Cell Factor 10 µg E SDF-1 alpha (CXCL12), rat, recombinant SDF-1 beta (CXCL12), rat, recombinant Recombinant Rat Stromal Cell Derived Factor 1-alpha (CXCL12) Recombinant Rat Stromal Cell Derived Factor 1-beta (CXCL12) 10 µg E µg E TNF-alpha, rat, recombinant Recombinant Rat Tumor Necrosis Factor alpha 20 µg E VEGF, rat, recombinant Recombinant Rat Vascular Endothelial Growth Factor 10 µg E VEGF-C, rat, recombinant Recombinant Rat Vascular Endothelial Growth Factor C 10 µg E VEGF-C152S, rat, recombinant Recombinant Rat Vascular Endothelial Growth Factor C (152 aa) 20 µg E Cytokines and Growth Factors * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

188 186 7 Cytokines and Growth Factors 7.2 CD Antigens n Human und Mouse Product Name Product Description Size* Catalog Number CD1A, human, recombinant Recombinant Human CD1A 2 µg C CD2, human, recombinant Recombinant Human CD2 2 µg C CD4, human, recombinant Recombinant Human soluble CD4 50 µg C CD7, human, recombinant Recombinant Human CD7 2 µg C CD9, human, recombinant Recombinant Human CD9 2 µg C CD10, human, recombinant Recombinant Human CD10 2 µg C CD11b, human, recombinant Recombinant Human CD11b 2 µg C CD14, human, recombinant Recombinant Human CD14 2 µg C CD14, human, recombinant (CHO) Recombinant Human CD14 (CHO) 2 µg C CD14, mouse, recombinant (CHO) Recombinant Mouse CD14 (CHO) 2 µg D CD21, human, recombinant Recombinant Human CD21 2 µg C CD22, human, recombinant (CHO) Recombinant Human CD22 (CHO) 20 µg C CD23, human, recombinant Recombinant Human CD23 2 µg C CD25, human, recombinant Recombinant Human CD25 (sil-2 Receptor alpha) 25 µg C CD29, human, recombinant Recombinant Human CD29 2 µg C CD31, human, recombinant Recombinant Human CD31 2 µg C CD34, human, recombinant Recombinant Human CD34 2 µg C CD38, human, recombinant Recombinant Human CD38 2 µg C CD43, human, recombinant Recombinant Human CD43 2 µg C CD45, human, recombinant Recombinant Human CD45 2 µg C CD52, human, recombinant Recombinant Human CD52 2 µg C CD56, human, recombinant Recombinant Human CD56 2 µg C CD68, human, recombinant Recombinant Human CD68 2 µg C CD72, human, recombinant Recombinant Human CD72 2 µg C CD95, human, recombinant Recombinant Human CD95 (sfas Receptor) 20 µg C CD99, human, recombinant Recombinant Human CD99 2 µg C CD105, human, recombinant (Sf9) Recombinant Human soluble CD105 (Endoglin, Sf9) 25 µg C CD105, human, recombinant (E. coli) Recombinant Human soluble CD105 (Endoglin; E. coli) 5 µg C CD105, mouse, recombinant Recombinant Mouse Soluble CD105 (Endoglin; Sf9) 25 µg D CD120a, human, recombinant Recombinant Human CD120a (TNFR1) 20 µg C CD120b, human, recombinant Recombinant Human CD120b (TNFR2) 20 µg C CD124, human, recombinant Recombinant Human CD124 (sil-4 Receptor) 15 µg C Cytokines and Growth Factors CD126, human, recombinant Recombinant Human CD126 (sil-6 Receptor) 20 µg C CD137, human, recombinant Recombinant Human CD137 (4-1BB sreceptor) 20 µg C CD146, human, recombinant Recombinant Human CD146 2 µg C CD147, human, recombinant Recombinant Human CD147 2 µg C CD152, human, recombinant Recombinant Human CD152 (CTLA-4) 100 µg C CD154, human, recombinant Recombinant Human soluble CD154 (TRAP, CD40 Ligand) 50 µg C CD283, human, recombinant Recombinant Human CD283 (TLR3) 25 µg C * Other sizes (including bulk amounts) are also available! Please request! Abbreviations: Sf9 = Sf9 insect cell-derived, InC = Insect cell-derived, CHO = CHO cell-derived, HEK = HEK cell-derived, Yeast = Yeast cell-derived, Pichia = Pichia-derived, S. lividans = S. lividans-derived, E. coli = E. coli-derived, His = His-tagged, aa = amino acid variant

189 Microbial Detection 8 and Elimination Cell culture contamination is a commonly occurring problem that affects many of the cell s biochemical and immunological properties, and it leads to unsatisfactory and unreliable results. Therefore, regularly testing cell cultures for contamination with mycoplasmas/bacteria is essential. PromoKine provides highly sensitive and user-friendly test kits for this purpose as well as products for the effective elimination and prevention of mycoplasma/eubacteria contamination.

190 188 Contents Chapter 8 8 Microbial Detection and Elimination 8.1 Mycoplasma Test Kits Mycoplasma Elimination Solutions Bacteria Test Kit Disinfectants

191 8 Microbial Detection and Elimination Mycoplasma Test Kits Applications Mycoplasma detection in cell culture, cell/virus stocks, and medium Conventional and real-time PCR Features Highly sensitive (detects mycoplasmas per sample volume) Fast, reproducible, and reliable results Applicable for all cell/tissue culturerelevant mycoplasma species Positive and internal controls included Convenient protocol and easy sample preparation Mycoplasmas are parasitic bacteria that frequently contaminate tissue cultures and consequently also virus stocks, vaccines, and other biological materials produced in cells. The contamination can originate from serum, laboratory staff, other contaminated cultures, or the donors from which the cells have been harvested, and it often remains unnoticed. Although mycoplasmas do not cause visible cell damage, they seriously affect cell growth and metabolism, transfection efficiency, protein and monoclonal antibody synthesis, cytokine secretion, immunological properties, signal transduction, virus proliferation and can even cause DNA and RNA damage. Since mycoplasmas are very small and lack cell walls, detection using a conventional light microscope is nearly impossible. In addition, cloudiness in the medium, typical for bacterial or yeast contaminations, does not occur even at high mycoplasma concentrations. PCR on the other hand is a highly sensitive, specific, and rapid method for the detection of mycoplasma contamination in culture supernatant, cell/virus stocks, and medium. All cell/ tissue culture relevant mycoplasma species can be detected in one assay including M. fermentans, M. hyorhinis, M. arginini, M. orale, M. salivarium, M. hominis, M. pulmonis, and M. pirum. In addition, M. pneumoniae, Acholeplasma laidlawii, M. synoviae, and Ureaplasma species can be detected. PCR Mycoplasma Test Kit I/C The PCR Mycoplasma Test Kit I/C utilizes conventional PCR technology for convenient and specific detection of more than 25 contaminating mycoplasma species. The primer set is specific for the highly conserved 16S-rRNA coding region in the mycoplasma genome. The test can be performed in 3 hours and includes internal control DNA (for verifying a successful PCR run) as well as positive control DNA. All components required for the PCR (primers, nucleotides, internal control DNA) come optimally premixed and lyophilized in PCR tubes (except the Taq polymerase). For setting up the PCR, simply cut off the number of tubes required, rehydrate with rehydration buffer (containing the Hot-Start Taq polymerase), and add the sample to be analyzed. Samples can be easily prepared by boiling the cell culture supernatant or other sample material. PCR Mycoplasma Test Kit I/RT The PCR Mycoplasma Test Kit I/RT utilizes real-time PCR technology for convenient and reproducible detection of contaminating mycoplasmas. It shows excellent sensitivity and specificity and enables reliable, quantitative PCR results extremely fast, particularly for routine testing for mycoplasma contamination. The primer and probe set is specific for the highly conserved 16S-rRNA coding region in the mycoplasma genome. It contains FRET probes emitting fluorescent light at 520 nm and is compatible with most common Real-Time PCR cyclers (e.g. Roche Light Cycler 1 and 2, ABI Prism, Cepheid Smart Cycler, and Eppendorf Mastercycler ep realplex). The test includes internal control DNA (for verifying a successful PCR run) as well as positive control DNA. All components required for real time PCR (primers, probes, nucleotides, and internal control DNA) come lyophilized in PCR tubes, except the Hot-Start Taq polymerase which is dissolved in reaction buffer. PCR 100 µl cell culture supernatant Boil for 10 minutes and centrifuge briefly 2 µl supernatant Analyze 5 µl of the PCR sample Fig. 1: Mycoplasma detection with the PromoKine PCR Mycoplasma Test Kit I/C is easy and convenient: Boil 100 µl cell culture supernatant or other sample material for 10 minutes; centrifuge briefly and add 2 µl supernatant to the reaction cup with the rehydrated PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis. When using the PCR Mycoplasma Test Kit II, the sample is directly added to the ready-to-use, complete reaction mix to set up the PCR. 8 Microbial Detection and Elimination

192 190 8 Microbial Detection and Elimination PCR Mycoplasma Test Kit II The PCR Mycoplasma Test Kit II utilizes conventional PCR technology for convenient and reliable detection of mycoplasmas. The primer set is specific for the highly conserved 16S-rRNA coding region in the mycoplasma genome. The kit includes all components required for PCR (nucleotides, primers, a high-performance Taq polymerase, magnesium, and an agarose gel loading dye) premixed at optimized concentrations in a ready-to-use master mix. The test takes approximately five hours to obtain clear results and reliably detects all mycoplasmas commonly found in cell cultures. The kit also contains positive control DNA for easy result verification but, different to the PCR Mycoplasma Test Kit I/C, no internal control DNA. M bp 270 bp Fig. 2: Detection of mycoplasmas with the PCR Mycoplasma Test Kit I/C. M: 100 bp DNA ladder 1: Positive control 2: 10 mycoplasmas/sample volume 3: 100 mycoplasmas/sample volume 4: 1,000 mycoplasmas/sample volume 5: 10,000 mycoplasmas/sample volume 6: 100,000 mycoplasmas/sample volume Internal control DNA: 479 bp band Positive sample/control: 270 bp band Product Size Catalog Number PCR Mycoplasma Test Kit I/C 24 tests 48 tests 96 tests PK-CA PK-CA PK-CA PCR Mycoplasma Test Kit I/RT 25 tests PK-CA PCR Mycoplasma Test Kit II 10 tests 20 tests PK-CA PK-CA We recommend screening tissue cultures monthly, especially before cryopreservation. 8.2 Mycoplasma Elimination Solutions 8 Microbial Detection and Elimination Mycoplasma contamination seriously affects cell growth and cell physiology leading to biased and unreliable experimental results. When mycoplasma contamination is detected, the contaminated cells are ideally discarded and all equipment, e.g. work benches, CO 2 incubators, etc., are disinfected (see chapter 8.4 for disinfectant sprays). However, if the affected cells are unique or the result of extensive work, thorough elimination of the contaminating mycoplasmas is necessary. Standard antibiotics commonly used in cell culture (e.g. Pen/Strep) do not affect mycoplasmas. Therefore, PromoKine offers two highly effective methods to get rid of mycoplasma contamination: Mycoplasma-EX, a combination of nonantibiotic and antibiotic reagents, and BIOMYC 1-3, three highly effective antibiotics. Fig. 3: Electron microscope image of cells contaminated with mycoplasmas. Picture kindly provided by M. Ebert, Forschungszentrum für Medizintechnik und Biotechnologie, Erfurt, Germany.

193 8 Microbial Detection and Elimination 191 Mycoplasma-EX Applications Mycoplasma elimination in cell/tissue culture Mycoplasma elimination in virus stocks Features Highly effective against mycoplasma species commonly found in cell cultures Very short treatment time Very low cytotoxicity Ready to use Easy handling Mycoplasma-EX combines a non-antibiotic and a thoroughly adjusted antibiotic treatment for the effective elimination of mycoplasmas and other microorganisms in cell/tissue culture and virus stocks. It eliminates mycoplasmas by directly killing them and not just by inhibiting their growth, while showing extremely low toxicity for the cells in culture. Mycoplasma-EX is effective with only one treatment, destroying mycoplasmas rapidly and permanently. It is applicable for most cell lines (e.g. Vero, BHK21, GBK, ML, Hep2, 293, CRFK, H9, Molt4, MT-4, Jurkat) as well as virus stocks (e.g. SHV-1, BHV-1, HSV-1, VSV, SFV, FCV, MEV) and highly effective against M. orale, M. hyorhinis, M. hominis, Acholeplasma laidlawii, M. arginini, M. fermentans, and other mycoplasma species usually encountered as contaminants in cell cultures. BIOMYC Antibiotic Solutions Applications Mycoplasma elimination in cell/tissue culture Features Highly effective against mycoplasma species commonly found in cell cultures Low cytotoxicity Ready to use Easy handling Antibiotics that affect bacterial cell walls (e.g. penicillin) are ineffective against mycoplasmas, which lack a cell wall. Other antibiotics such as tylosin, neomycin, tetracycline, or gentamicin are only effective against a few mycoplasma species commonly found in cell cultures. In addition, they frequently just reduce the concentration of mycoplasmas rather than completely eliminating them. As soon as treatment is discontinued, contamination will recur. BIOMYC-1 and 2 BIOMYC-1 and 2 are two antibiotics, which eliminate mycoplasmas very efficiently when used in combination. After a four-day treatment with BIOMYC-1, a three-day treatment with BIOMYC-2 follows. This is repeated 2 to 3 times. Following this protocol, the mycoplasmas do not develop resistance to these antibiotics - a common occurrence with other antibiotic treatment methods. BIOMYC-1 is based on the antibiotic tiamutin which is produced by the fungus Pleurotus mutilus. BIOMYC-2 is based on the antibiotic minocycline, an improved derivative of tetracycline. BIOMYC-3 BIOMYC-3 is based on the antibiotic ciprofloxacin, which is a member of the fluoroquinolone group and inhibits synthesis of DNA gyrase, an enzyme necessary for bacterial DNA supercoiling. Many mycoplasma species are sensitive to BIOMYC-3, including M. orale, M. hyorhinis, M. fermentans, and M. arginini, but also Acheloplasma laidlawii. These species are responsible for most mycoplasma contaminations in cell cultures. At the concentrations recommended for use, no cytotoxic effects on the cells in culture have been found and the treatment can be performed within 12 days. Product Size Catalog Number Mycoplasma-EX 3 treatments PK-CC BIOMYC-1 BIOMYC-2 BIOMYC-3 10 ml 20 ml 100 ml 10 ml 20 ml 100 ml 10 ml 20 ml 100 ml PK-CC D PK-CC C PK-CC B PK-CC D PK-CC C PK-CC B PK-CC D PK-CC C PK-CC B 8 Microbial Detection and Elimination

194 192 8 Microbial Detection and Elimination 8.3 Bacteria Test Kit Applications Eubacteria detection in cell culture Eubacteria detection in cell/virus stocks Eubacteria detection in medium Features Highly sensitive (detects 12 eubacteria genomes per sample volume) Reproducible and reliable results Applicable for 45 eubacteria species Positive and internal controls included Convenient protocol and easy sample preparation Bacteria are frequently found as contaminants in cell cultures. However, cell cultures sometimes lack visual signs of bacterial contamination, e.g. fast decoloration of the medium. Moreover, it has been demonstrated that standard antibiotics are mostly ineffective against resistant bacterial infection but have a strong impact on the metabolism, growth, and differentiation of the cells in culture. Frequent screening of cell cultures for eubacteria contamination is indispensable to obtain reliable results. Therefore, PromoKine offers a PCR-based Bacteria Test Kit that has been developed for accurate and reliable in vitro diagnosis of 45 eubacteria species, many of them found as usual contaminants in cell cultures (e.g. Actinomyces, Bacillus, Enterococcus, Escherichia coli, Fusobacterium, Klebsiella, Lactobacillus, Micrococcus, Mycobacterium, Peptostreptococcus, Pseudomonas, Porphyromonas, Prevotella, Salmonella, Serratia, Staphylococcus, Streptococcus, etc.). The kit provides a highly sensitive, easy, and rapid method to detect eubacteria infection in various in situ biological materials, including cell cultures. Detection requires as little as 12 eubacteria genomes (~52 fg) per sample volume. The protocol can be completed within 3.5 hours with a hands-on time of less than 40 minutes. The kit contains all necessary components for setting up a PCR including a positive control DNA as well as an internal control DNA to confirm a successfully performed PCR run. The kit does not amplify eukaryotic DNA. PCR µl cell culture supernatant Extract Bacterial DNA 5 µl DNA sample Analyze 5 µl of the PCR sample Fig. 4: Eubacteria detection with the PromoKine PCR Bacteria Test Kit is easy and convenient: Purify bacterial DNA from µl cell culture supernatant by using an appropriate, commercial DNA extraction kit; add 5 µl sample DNA to the reaction cup with the premixed PCR components and Taq polymerase; start PCR run; analyze PCR products by agarose gel electrophoresis. Product Size Catalog Number PCR Bacteria Test Kit 48 tests PK-CA PromoKine also offers a Bacteria Live/Dead Staining Kit. Please see our website for more information. 8 Microbial Detection and Elimination

195 8 Microbial Detection and Elimination Disinfectants Disinfection of all tissue culture equipment and work surfaces in the lab has to be performed regularly to prevent contamination. PromoKine offers different disinfectants ideal for laboratory surfaces and apparatuses, CO 2 incubators, and water baths. Mycoplasma-EX Spray Applications Disinfection of all laboratory surfaces Disinfection of laboratory apparatuses Features Kills mycoplasmas within seconds Effective against most microorganisms Non-corrosive and non-cancerogenic Does not leave any residue Supplied in a refillable spray bottle Mycoplasma-EX Spray allows safe disinfection and cleaning of all laboratory surfaces and apparatuses, including clean and work benches, incubators, cell storage boxes, and liquid nitrogen containers. It utilizes the powerful Mycoplasma-EX reagent in combination with an alcohol-based solution. Besides its strong mycoplasmacidal activity, it is very effective against Staphylococcus aureus, Streptococcus faecalis, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Candida albicans, Mycobacteria, HBV/HIV, Papova, Adeno, Polio and Vaccinia viruses. The effectiveness of Mycoplasma-EX Spray is further enhanced by its excellent detergent properties, so that clean and contamination-free surfaces can be achieved in one step. Pharmacidal Spray Applications Disinfection of incubators Features Free of mercury, formadeldehyde, phenol and alcohol Very effective against bacteria, fungi, spores, and viruses Non-corrosive and non-cancerogenic Does not leave any residue Supplied in a refillable spray bottle Pharmacidal Spray is a mercury-, formaldehyde-, phenol-, and alcohol-free disinfectant particularly intended for the disinfection of incubators. It is recommended to spray the incubators thoroughly every two weeks. Aquaguard Applications Disinfection of water trays in CO 2 incubators Disinfection of water baths Features Very effective against bacteria, fungi, spores, and viruses Non-corrosive Non-toxic and non-volatile The water in an incubator or in a water bath is a major source of cell culture contamination, particularly with respect to mycoplasma contaminations, since mycoplasmas can survive in pure water. Aquaguard, a non-volatile disinfectant, is extremely effective in eliminating microorganisms in water. In addition, it does not cause damage to the stainless steel of the incubator or any plastic interior, and it does not cause any skin irritation when used as recommended. Aquaguard-1 is intended for disinfecting water in incubators, whereas Aquaguard-2 is intended for water bath disinfection. Product Size Catalog Number Mycoplasma-EX Spray 500 ml PK-CC Pharmacidal Spray 1000 ml PK-CC Aquaguard ml PK-CC B Aquaguard-2 50 ml PK-CC E 8 Microbial Detection and Elimination

196 194 Alphabetical Index Alphabetical Index Description Page Description Page 1-9 1,25-Dihydroxy Vitamin D ELISA Kit, human X Annexin V Binding Buffer 35 1-Methoxy PMS 8 1X Annexin V Binding Buffer OH Vitamin D (direct) ELISA Kit, human 162 2K antibody (mab) BB sreceptor (CD137), human, recombinant BBL (CD137L), human, recombinant BBL antibody BBR antibody 89 4-Bromo A free acid 22 4E-BP1 antibody 114 5(6)-Carboxy-2',7'-dichlorofluorescein 21 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate 21 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate, succinimidyl ester 5(6)-Carboxyfluorescein 21 5(6)-CFDA 21 5(6)-CFDA, SE 21 5,5'-dibromo-Bapta (tetrapotassium salt) 20 5,5'-difluoro-Bapta (tetrapotassium salt) 20 5,5'-difluoro-Bapta AM 20 5,5'-dimethyl-BAPTA (tetrapotassium salt) 20 5,5'-dimethyl-BAPTA AM 20 5-Carboxy-2',7'-dichlorofluorescein 21 5-Carboxy-2',7'-dichlorosulfonefluorescein 22 5-CFDA 22 5-FAM 22 5-methyl-5'-nitro-BAPTA (tetrapotassium salt) 20 5'-mononitro-BAPTA (tetrapotassium salt) 20 6-CFDA 22 6-Ckine ELISA Kit, mouse FAM 22 90K (Mac-2BP) ELISA Kit, human K/Mac-2BP antibody (mab) 89 90K/Mac-2BP antibody (mab), FITC 89 a1-antitrypsin (a1-proteinase Inhibitor) ELISA Kit, human 162 a1-microglobulin (a1-m) ELISA Kit, human 162 A free acid 22 a2-macroglobulin ELISA Kit, human 162 AATF / Che-1 antibody 120 ACE2 (CT) antibody 137 ACE2 (IN1) antibody 137 ACE2 (IN2) antibody 137 ACE2 (NT) antibody 137 Acetyl-Histone H2A antibody 120 Acetyl-Histone H2B antibody 120 Acetyl-Histone H3 antibody 120 Acetyl-Histone H4 antibody 120 A 21 Acinus (CP) antibody 120 Acinus (CT) antibody 120 Acinus (IN) antibody 120 Acinus (NP) antibody 120 Acridine Orange (AO) Solution 13 Acrp30 (E. coli), human, recombinant 169 Acrp30 (HEK), human, recombinant 169 Acrp30 (His), human, recombinant 169 Acrp30 (Sf9), human, recombinant 169 Acrp30, mouse, recombinant (E. coli) 181 Acrp30, mouse, recombinant (HEK) 181 Actinomycin D 43 Actinomycin D (10 mm) 43 Activin A, human, recombinant 169 Activin B, human, recombinant 169 ADAM10 / KUZ (CT) antibody 120 ADAP (CT) antibody 146 ADAP (NT) antibody 146 Adipolean Variant antibody 89 Adiponectin / Acrp30 (CT) antibody 89, 114 Adiponectin / Acrp30 (NT) antibody 89, 114 Adiponectin / Acrp30 antibody (mab) 89, 144 Adiponectin ELISA Kit, human 154 ADMA direct ELISA Kit, human 162 ADMA direct ELISA Kit, mouse/rat 162 ADVASEP-7 (sulfobutyl b-cyclodextrin) 15 AES / GRG / ESP1 (CT) antibody 120 AES / GRG / ESP1 (NT) antibody 120 AFHA-2 antibody 138 AFHA-3 antibody 139 AFHA-4 antibody 139 AID antibody 89 AIF (CT) antibody 120 AIF (IN) antibody 120 AIF (NT) antibody 120 AIFM3 (IN) antibody 120 AIFM3 (NT) antibody 120 AIM / API6 (CT) antibody 120 AIM / API6 (NT) antibody 120 AITRL antibody 89 AITRL, human, recombinant 169 AK Cytotoxicity Kit 10 Akt Activated Cell Lysate 41 Akt Activity Detection Kit 41 Akt Inhibitor 41 Akt Negative Control Cell Lysate 41 Akt1 / PKB antibody 114 Alkaline Phosphatase Labeling Kit-NH2 152 Alkaline Phosphatase Labeling Kit-SH 152 Allophycocyanin (cross-linked), lyophilized 56 Allophycocyanin Labeling Kit-NH 2 57 Allophycocyanin Labeling Kit-NH Allophycocyanin Labeling Kit-SH 57 Allophycocyanin Labeling Kit-SH 153

197 Alphabetical Index 195 Description Page Description Page Allophycocyanin, lyophilized 56 Alpha-Bungarotoxin (from Bungarus multicinctus) 15 AM AM AM AM AM AM AM AM Ambra1 antibody (CT) 148 Ambra1 antibody (NT) 148 Aminopeptidase P (epithelial) antibody (mab) 150 Ang-1 antibody (mab) 89 Ang-1, human, recombinant (Sf9) 169 Ang-2 antibody (mab) 89 Ang-2, human, recombinant (Sf9) 169 Angiogenin ELISA Kit, human 154 Angiopoietin-1 ELISA Kit, human 154 Angiostatin ELISA Kit, human 154 ANGPTL-3, human, recombinant 169 ANGPTL-4, human, recombinant 169 Anisomycin 43 Annexin V antibody (mab) 120 Annexin V antibody (mab), biotin 120 Annexin V antibody (mab), FITC 120 Annexin V antibody 120 Annexin V ELISA Kit, human 161 Annexin V Unlabeled Reagent 35 Annexin V-Biotin Detection Kit 34 Annexin V-Biotin Reagent 35 Annexin V-Cy3 Detection Kit 34 Annexin V-Cy3 Detection Kit Plus 34 Annexin V-Cy3 Reagent 35 Annexin V-Cy5 Detection Kit 34 Annexin V-Cy5 Reagent 35 Annexin V-EGFP Detection Kit 34 Annexin V-EGFP Reagent 35 Annexin V-FITC Detection Kit 34 Annexin V-FITC Detection Kit Plus 35 Annexin V-FITC Reagent 35 Annexin V-PE Detection Kit 35 Annexin V-PE Detection Kit Plus 35 Annexin V-PE Reagent 35 Annexin V-PE-Cy5 Detection Kit 35 Annexin V-PE-Cy5 Reagent 35 Anthrax EF antibody 138 Anthrax LF (CT) antibody 138 Anthrax LF (IN) antibody 138 Anthrax LF antibody 138 Anthrax PA (CT) antibody 138 Anthrax PA (IN) antibody 138 Anti-Annexin V ELISA Kit, human 161 Anti-IFN-alpha ELISA Kit, human 154 Apaf-1 (CT) antibody 120 Apaf-1 (NT) antibody 120 APG7 / ATG7 (CT) antibody 121 APG7 / ATG7 (NT) antibody 121 APH1 (IN) antibody 131 APH1 (NT) antibody 131 Apo A1 antibody 89 Apo B antibody 89 Apo E3 antibody 89 APO-1/Fas (2R2) antibody (mab) 121 APO-1/Fas (APO-1-1) antibody (mab) 121 APO-1/Fas (APO-1-1) antibody (mab), biotin 121 APO-1/Fas (APO-1-1) antibody (mab), FITC 121 APO-1/Fas (SM1/1) antibody (mab) 121 APO-1/Fas (SM1/23) antibody (mab) 121 APO-1/Fas (SM1/23) antibody (mab), biotin 121 APO-1/Fas (SM1/23) antibody (mab), FITC 121 APO-A1, human, recombinant 169 APOBEC3G (NT) antibody 135 APO-E2, human, recombinant 169 APO-E3, human, recombinant 169 APO-E4, human, recombinant 169 Apolipoprotein-J, rat, recombinant 184 Apoptosis Inducer Set 43 Apoptotic Cell Isolation Kit 43 Apoptotic DNA Ladder Detection Kit I 31 Apoptotic DNA Ladder Detection Kit II 31 Apoptotic DNA Ladder Detection Kit I plus 31 Apoptotic/Necrotic Cells Detection Kit 42 Apoptotic/Necrotic/Healthy Cells Detection Kit 42 Apo-SAA antibody 89 Apo-SAA, human, recombinant 169 APP (CT) antibody 131 APP / AβNT antibody 131 APRIL (ED) antibody 90 APRIL (ED2) antibody 90 APRIL antibody 90 APRIL ELISA Kit, human 154, 161 APRIL, mouse, recombinant 181 Aquaguard Aquaguard ARC (CT) antibody 121 ARC (NT) antibody 121 ARF-BP1 antibody 121 ARMER / ARL6IP / AIP1 (CT) antibody 121 Artemin antibody 90 Artemin, human, recombinant 169 ARTS antibody 121 ASC antibody 121 ASK1 / MAPKKK5 (C2) antibody 121 ASK1 / MAPKKK5 (CT) antibody 121 ASK1 / MAPKKK5 (NT) antibody 121 ATF6 (CT) antibody 114 ATF6 (NT) antibody 114

198 196 Alphabetical Index Description Page Description Page ATG10 antibody 148 ATG12 (IN) antibody 148 ATG12 (NT) antibody 148 ATG16 (IN) antibody 148 ATG16 (NT) antibody 148 ATG5 antibody 148 ATM antibody 141 ATR / TEM8 (CT) antibody 138 ATR / TEM8 (IN) antibody 138 Aven (CT) antibody 121 Aven (NT) antibody 121 Avian Influenza A M2 antibody 139 AXL ELISA Kit, human 154 AXL ELISA Kit, mouse 154 B220 antibody (mab) 90 b2-microglobulin (b2-m) ELISA Kit, human 162 B-71 antibody 90 BACE / Asp2 (CT) antibody 131 BACE2 / Asp1 (CT) antibody 131 BACE2 / Asp1 (NT) antibody 131 Bad / Bcl-2-like 8 antibody 121 BAFF (CT) antibody 90 BAFF antibody (mab) 90 BAFF antibody 90 BAFF ELISA Kit, human 154, 161 BAFF, human, recombinant 169 BAFF-R / BR3 (CT) antibody 90 BAFF-R, human, recombinant 169 BAG-1 / RAP46 (CT) antibody 121 BAG-1 / RAP46 (NT) antibody 121 Bak (NT) antibody 121 BANF1 (CT) antibody 135 BANF1 (NT) antibody 135 BAP29 (CT) antibody 114 BAP29 (IN) antibody 114 BAP3 (NT) antibody 131 BAP3 (NT2) antibody 131 BAP31 (CT) antibody 114 BAP31 (IN) antibody 114 Bapta (tetracesium salt) 20 Bapta (tetrapotassium salt) 20 Bapta (tetrasodium salt) 20 Bapta AM 20 Bapta-FF (free acid) 20 Bapta-FF AM 20 Bax (NT) antibody 122 Bax antibody (mab) 122 BCA-1 (BLC, CXCL13), human, recombinant 169 BCA-1 (BLC, CXCL13), mouse, recombinant 181 BCA-1 antibody 90 B BCECF 14, 22 BCECF AM 14, 22 BCECF AM Solution 14, 22 Bcl-10 / CIPER / CLAP (NT) antibody 122 Bcl-2 (IN) antibody 122 Bcl-2 (NT) antibody 122 Bcl-2 antibody (mab) 122 Bcl-2 ELISA Kit, human 161 Bcl-B / Bcl-2L10 antibody 122 Bcl-G / Bcl-2-like 14 (CT) antibody 122 Bcl-rambo / Mil1 (IN) antibody 122 Bcl-xL antibody 122 BCMA antibody 90 BCMA, human, recombinant 169 BD-1 (36 aa), human, recombinant 169 BD-1 (47 aa), human, recombinant 169 BD-1 antibody 90 BD-2 antibody 90 BD-2 EDK, human 163 BD-2, human, recombinant 169 BD-3 antibody 90 BD-3 EDK, human 163 BD-3, human, recombinant 169 BD-4 antibody 90 BD-4, human, recombinant 169 BDNF antibody (mab) 90 BDNF antibody 90 BDNF ELISA Kit, human 154 BDNF, human, recombinant 169 Beclin-1 (CT) antibody 122 Beclin-1 (NT) antibody 122 beta-actin (CT) antibody 89, 141 beta-actin (NT) antibody 89, 141 beta-actin antibody (mab) 89, 141 beta-actin antibody 89, 141 Betacellulin antibody 90 Betacellulin EDK, human 163 Beta-Galactosidase Reporter Assay Kit 12 Beta-Galactosidase Staining Kit 12 beta-ngf antibody (mab) 107 beta-ngf antibody 107 beta-ngf EDK, human 165 beta-ngf, human, recombinant (CHO) 177 beta-ngf, mouse, native 181 Beta-Secretase Fluorometric Assay Kit 23 Beta-Secretase Inhibitor I 23 Beta-Secretase Inhibitor II 23 Betulinic acid 43 Bfl-1 (CT) antibody 122 Bfl-1 (NT) antibody 122 BICD1 (CT) antibody 131 Bid (CT) antibody 122 Bid (IN) antibody 122

199 Alphabetical Index 197 Description Page Description Page Bif antibody 122 Big Endothelin ELISA Kit, human 162 Bik antibody 122 Bim / BOD (IN) antibody 122 Bim / BOD (IN2) antibody 122 Biocytin (e-biotinoyl L-lysine) 17 Biocytin-Hydrazide 17 Bioluminescent Cell Viability Kit I (ATP) 8 Bioluminescent Cell Viability Kit II (ADP/ATP) 8 BIOMYC BIOMYC BIOMYC Biotin Labeling Kit-NH Biotin Labeling Kit-SH 153 Biotin-4-Fluorescein 17 Biotin-Allophycocyanin 58 Biotin-Allophycocyanin (cross-linked) 58 Biotin-AM Biotin-B-Phycoerythrin 58 Biotin-DHPE 15 Biotin-Ethylenediamine, hydrobromide 17 Biotin-Ethylenediamine, hydrochloride 17 Biotin-Rhodamine Biotin-R-Phycoerythrin 58 Biotin-X-DHPE 15 Biotin-XX-a-bungarotoxin 16 Bis-Fura-2 (hexapotassium salt) 20 Bit1 (IN) antibody 122 Bit1 (NT) antibody 122 BLC antibody 90 BLC ELISA Kit, human 154 BLC ELISA Kit, mouse 154 Blimp-1 (CT) antibody 146 Blimp-1 (NT) antibody 146 Blood DNA Isolation Buffer 27 Blue-fluorescent Cytoplasmic Membrane Staining Kit 14 Bmf (CT) antibody 122 Bmf (NT) antibody 122 BMI-1 (IN) antibody 131 BMP-1 antibody 90 BMP-13 (CDMP-2), human, recombinant 170 BMP-13 antibody 91 BMP-14 (CDMP-1), human, recombinant 170 BMP-14 antibody 91 BMP-2 antibody (mab) 90 BMP-2 antibody 90 BMP-2 EDK, human 163 BMP-2, human, recombinant 169 BMP-3 antibody 90 BMP-4 antibody (mab) 90 BMP-4 antibody 90 BMP-4 ELISA Kit, human 154 BMP-4, human, recombinant 169 BMP-5 antibody 91 BMP-6 antibody 91 BMP-6 ELISA Kit, human 154 BMP-6, human, recombinant 169 BMP-7 antibody (mab) 91 BMP-7 antibody 91 BMP-7 ELISA Kit, human 154 BMP-7, human, recombinant 170 BMP-8 antibody 91 Bnip3L / Nix (IN) antibody 122 BNP, human, recombinant 170 Bonzo / STRL33 (CT) antibody 135 Bonzo / STRL33 (NT2) antibody 135 B-Phycoerythrin Labeling Kit-NH 2 57, 153 B-Phycoerythrin Labeling Kit-SH 57, 153 B-Phycoerythrin, lyophilized 56 BRAK (CXCL14), human, recombinant 170 BRAK antibody 91 BRAL1 antibody 131 BRCC36 antibody 141 BRCC45 antibody 141 Brefeldin A 43 BRSK1 (CT) antibody 131 BRSK1 (IN) antibody 131 BRSK2 antibody 132 BSA antibody 91 BSP ELISA Kit, human 162 Bst2 antibody (NT) 135 BTC, human, recombinant 170 BTK / ATK / BPK (NT) antibody 140 Bub1 (CT) antibody 141 Bub1 (NT) antibody 141 Bub3 (CT) antibody 141 C-10 (CCL6), mouse, recombinant 181 C-10 antibody 91 C3aR / CD88 antibody 91 C3aR antibody 91 C5a antibody 91 CAD / DFF40 (CT) antibody 123 CAD / DFF40 (I17) antibody 123 Cadherin antibody 91 Calcein AM 14, 17 Calcein AM Solution 14 Calcein AM Solution, 1 mg/ml in anhydrous DMSO 17 Calcein AM Solution, 4 mm DMSO solution 17 Calcein Blue 21 Calcein, high purity 14, 17 Calcium Calibration Buffer Kit 22 Calpain-2 antibody 123 Calpain-3 antibody 123 C

200 198 Alphabetical Index Description Page Description Page Calpain-5 antibody 123 Calpain-6 antibody 123 Calpain-7 antibody 123 Calpain Activity Assay Kit 40 Calpain I antibody 123 Calpain Inhibitor Z-LLY-FMK 40 Calprotectin (MRP 8/14) ELISA Kit, human 162 camp Immunoassay Kit 23 Camptothecin 43 Camptothecin (2 mm) 44 CAPS1 (CT) antibody 132 CAPS1 (NT) antibody 132 CAPS2 (IN) antibody 132 Carabin (CT) antibody 147 Carabin (NT) antibody 147 CARD10 / CARMA3 antibody 123 CARD8 / TUCAN antibody 123 CARD9 antibody 123 Cardiotrophin-1 (CT-1) antibody 91 Cardiotrophin-1 (CT-1), human, recombinant 170 Cardiotrophin-1 (CT-1), mouse, recombinant 181 CARMA1 antibody 123 Caspase Screening Kit (FC) 37 Caspase Staining Kit Wash Buffer 36 Caspase-1 / ICE (CT) antibody 123 Caspase-1 / ICE (IN) antibody 123 Caspase-1 / ICE antibody 123 Caspase-1 Colorimetric Assay Kit 39 Caspase-1 Drug Screening Kit 37 Caspase-1 Fluorometric Assay Kit 39 Caspase-10 / FLICE2 (CT) antibody 124 Caspase-10 Colorimetric Assay Kit 39 Caspase-10 Drug Screening Kit 37 Caspase-10 Fluorometric Assay Kit 39 Caspase-11 antibody 124 Caspase-12 (IN) antibody 124 Caspase-12 (lrg) antibody 125 Caspase-12 (NT) antibody 125 Caspase-12 (sml) antibody 125 Caspase-12 antibody 125 Caspase-12 Fluorometric Assay Kit 39 Caspase-13 / ERICE (CT) antibody 125 Caspase-13 / ERICE antibody 125 Caspase-14 / MICE antibody 125 Caspase-14 antibody (mab) 125 Caspase-2 / ICH-1 (CT) antibody 123 Caspase-2 / ICH-1 (NT) antibody 123 Caspase-2 / ICH-1 antibody (mab) 123 Caspase-2 Colorimetric Assay Kit 39 Caspase-2 Drug Screening Kit 37 Caspase-2 Fluorometric Assay Kit 39 Caspase-3 (active) antibody 123 Caspase-3 Activity Detection Kit 39 Caspase-3 antibody (mab) 123 Caspase-3 antibody 123 Caspase-3 Colorimetric Assay Kit 39 Caspase-3 Drug Screening Kit 37 Caspase-3 Fluorometric & Colorimetric Assay Kit 39 Caspase-3 Fluorometric Assay Kit 39 Caspase-3 Fluorometric Assay Kit (HTS) 39 Caspase-4 / ICH-2 (IN) antibody 123 Caspase-4 / ICH-2 (NT) antibody 123 Caspase-4 / ICH- 2 antibody (mab) 123 Caspase-4 Colorimetric Assay Kit 39 Caspase-4 Drug Screening Kit 37 Caspase-4 Fluorometric Assay Kit 39 Caspase-5 / ICH-3 (IN) antibody 123 Caspase-5 / ICH-3 (NT) antibody 123 Caspase-5 / ICH-3 antibody 123 Caspase-5 Colorimetric Assay Kit 39 Caspase-5 Drug Screening Kit 37 Caspase-5 Fluorometric Assay Kit 39 Caspase-6 (active) antibody 123 Caspase-6 / Mch2 (IN) antibody 124 Caspase-6 / Mch2 (NT) antibody 124 Caspase-6 antibody 124 Caspase-6 Colorimetric Assay Kit 39 Caspase-6 Drug Screening Kit 37 Caspase-6 Fluorometric Assay Kit 39 Caspase-7 / ICELAP3 (CT) antibody 124 Caspase-7 / ICELAP3 (NT) antibody 124 Caspase-7 Activity Detection Kit 39 Caspase-7 antibody (mab) 124 Caspase-7 Drug Screening Kit 37 Caspase-8 (active) antibody (mab) 124 Caspase-8 (active) antibody 124 Caspase-8 / FLICE (CT) antibody 124 Caspase-8 / FLICE (IN) antibody 124 Caspase-8 / FLICE ELISA Kit, human 162 Caspase-8 antibody (mab) 124 Caspase-8 antibody 124 Caspase-8 Colorimetric Assay Kit 39 Caspase-8 Drug Screening Kit 37 Caspase-8 Fluorometric & Colorimetric Assay Kit 39 Caspase-8 Fluorometric Assay Kit 39 Caspase-8 Fluorometric Assay Kit (HTS) 39 Caspase-8 / FLICE antibody (mab) 124 Caspase-9 (active) antibody 124 Caspase-9 / Apaf-3 (IN1) antibody 124 Caspase-9 / Apaf-3 (IN2) antibody 124 Caspase-9 / Apaf-3 antibody 124 Caspase-9 Colorimetric Assay Kit 39 Caspase-9 Drug Screening Kit 37 Caspase-9 ELISA Kit, human 162 Caspase-9 Fluorometric Assay Kit 39 Cathelicidin antibody 147

201 Alphabetical Index 199 Description Page Description Page Cathepsin B & L Substrate Z-FR-R Cathepsin B Activity Assay Kit 40 Cathepsin B antibody 144 Cathepsin B&L Inhibitor Z-Phe-Phe-FMK 40 Cathepsin D Activity Assay Kit 40 Cathepsin D antibody 144 Cathepsin F antibody 144 Cathepsin G antibody 144 Cathepsin H Activity Assay Kit 40 Cathepsin H antibody 144 Cathepsin K Activity Assay Kit 40 Cathepsin K antibody 144 Cathepsin K ELISA Kit, human 162 Cathepsin L (cleaved) antibody 144 Cathepsin L Activity Assay Kit 40 Cathepsin L antibody (mab) 144 Cathepsin L antibody (mab), biotin 144 Cathepsin L antibody (mab), FITC 144 Cathepsin L antibody 144 Cathepsin L ELISA Kit, human 163 Cathepsin P antibody 144 Cathepsin S Activity Assay Kit 40 Cathepsin S antibody 144 Cathepsin Substrate Z-FR-AMC (Z-FR-MCA) 40 Cathepsin V antibody (mab) 144 Cathepsin V antibody 144 Cathepsin W antibody (mab) 145 Cbl antibody 114 CCDC98 (NT) antibody 141 CCR1 antibody 91 CCR10 antibody 91 CCR3 (CT) antibody 91, 135 CCR3 (NT) antibody 91, 135 CCR4 antibody 91 CCR5 (NT) antibody 91, 136 CCR8 (EL) antibody 91, 136 CD10, human, recombinant 170, 186 CD105, human, recombinant (E. coli) 186 CD105, human, recombinant (Sf9) 186 CD105, mouse, recombinant 186 CD11a antibody (mab) 92 CD11b antibody (mab), FITC 92 CD11b, human, recombinant 170, 186 CD11c / p150,95 antibody (mab) 92 CD11c antibody (mab) 92 CD120a, human, recombinant 186 CD120b, human, recombinant 186 CD124, human, recombinant 186 CD126, human, recombinant 186 CD137, human, recombinant 186 CD14, human, recombinant 186 CD14, human, recombinant (CHO) 170, 186 CD14, human, recombinant (E. coli) 170 CD14, mouse, recombinant (CHO) 181, 186 CD146, human, recombinant 170, 186 CD147, human, recombinant 171, 186 CD152 (CTLA-4), human, recombinant 171, 186 CD154, human, recombinant 186 CD1A (T6, LEU6) antibody (mab) 91 CD1A, human, recombinant 170, 186 CD2 (T11, LFA-2) antibody (mab) 91 CD2, human, recombinant 170, 186 CD21, human, recombinant 170, 186 CD22, human, recombinant (CHO) 170, 186 CD22L antibody 92 CD23, human, recombinant 170, 186 CD25 antibody (mab) 92 CD25, human, recombinant 186 CD26 antibody (mab) 92 CD26 antibody (mab), biotin 92 CD26 antibody (mab), FITC 92 CD27 antibody 92 CD28 antibody (mab) 92 CD283, human, recombinant 186 CD29, human, recombinant 170, 186 CD3 antibody (mab) 91 CD3 antibody (mab), biotin 91 CD3 antibody (mab), FITC 91 CD30 ELISA Kit, human 162 CD30L ELISA Kit, mouse 154 CD31 antibody (mab) 92 CD31, human, recombinant 170, 186 CD34 antibody (mab) 92 CD34, human, recombinant 170, 186 CD38, human, recombinant 170, 186 CD4 antibody (mab) 92 CD4 antibody (mab), biotin 92 CD4 antibody (mab), FITC 92 CD4, human, recombinant 186 CD40 antibody (mab) 92 CD40 antibody 92 CD40 ELISA Kit, human 162 CD40L antibody (mab) 92 CD40L antibody (mab), biotin 92 CD40L antibody (mab), FITC 92 CD40L antibody 92 CD40L ELISA Kit, human 154, 162 CD40L ELISA Kit, mouse 154 CD43, human, recombinant 170, 186 CD44std antibody (mab) 93 CD44std antibody (mab), biotin 93 CD44std antibody (mab), FITC 93 CD44var (v10) antibody (mab) 94 CD44var (v3) antibody (mab) 93 CD44var (v3) antibody (mab), biotin 93 CD44var (v3-v10) antibody 94 CD44var (v4) antibody (mab) 93 CD44var (v4) antibody (mab), FITC 93

202 200 Alphabetical Index Description Page Description Page CD44var (v5) antibody (mab) 93 CD44var (v5) antibody (mab), biotin 93 CD44var (v6) antibody (mab) 93 CD44var (v6) antibody (mab), biotin 93 CD44var (v6) antibody (mab), FITC 93 CD44var (v7) antibody (mab) 93 CD44var (v7) antibody (mab), FITC 93 CD44var (v7-v8) antibody (mab) 93 CD44var (v7-v8) antibody (mab), biotin 94 CD44var (v7-v8) antibody (mab), FITC 94 CD45, human, recombinant 170, 186 CD5 antibody (mab) 92 CD52, human, recombinant 170, 186 CD56, human, recombinant 170, 186 CD62E antibody (mab) 94 CD68, human, recombinant 170, 186 CD7, human, recombinant 170, 186 CD72, human, recombinant 170, 186 CD8 antibody (mab) 92 CD8 antibody (mab), biotin 92 CD8 antibody (mab), FITC 92 CD80 / B7-1 antibody (mab) 94 CD86 / B7-2 antibody (mab) 94 CD8a antibody (mab) 92 CD9, human, recombinant 170, 186 CD90 (Thy-1) antibody (mab) 94 CD90 (Thy-1.1) antibody (mab) 94 CD90 (Thy-1.2) antibody (mab) 94 CD95, human, recombinant 186 CD99, human, recombinant 170, 186 CDKN2A antibody 114 CDNF (CT) antibody 132 CDNF (NT) antibody 132 CellClean 85 CFSE; 5(6)-CFDA, SE 14 cgmp Immunoassay Kit 23 Chemiluminescence Detection Kit 153 Chk2 (NT) antibody 131 Chlamydia LPS antibody (mab) 149 Chromogenic beta-galactosidase Substrate Sampler Kit 12 ciap (CT) antibody 125 CIDE-A (CT) antibody 125 CIDE-B (C2) antibody 125 CIDE-B (CT) antibody 125 CIDE-B (IN) antibody 125 CIKS / Act1 (CT) antibody 115 CIKS / Act1 (NT) antibody 115 c-jun (1-79)-GST Fusion Protein 41 c-jun antibody 115 CLIP170 antibody 141 Clusterin / CLI / ApoJ antibody 132 CNTF antibody 94 CNTF ELISA Kit, human 154 CNTF, human, recombinant 171 CNTF, rat, recombinant 184 Coelenterazine (native) 12, 21 Coelenterazine 400a 21, 21 Coelenterazine cp 12, 20 Coelenterazine e 12, 21 Coelenterazine f 12, 21 Coelenterazine fcp 12, 21 Coelenterazine h 12, 21 Coelenterazine hcp 12, 21 Coelenterazine i 12, 21 Coelenterazine ip 12, 20 Coelenterazine n 12, 20 Coelenterazine Sampler Kit 12, 21 Coelenterazine, 2-methyl 12, 20 Coelenterazine-WS 12, 21 Colchicine 44 Colorimetric Cell Viability Kit I (WST-8) 8 Colorimetric Cell Viability Kit II (WST-1) 8 Colorimetric Cell Viability Kit III (XTT) 8 Colorimetric Cell Viability Kit IV (MTT) 8 Colorimetric DNA Fragmentation Detection Kit (IHC) 31 Colorimetric Glutathione Detection Kit 33 Colorimetric Glutathione Reductase Activity Kit 33 Colorimetric GST Assay Kit 25 Colorimetric Nitric Oxide Assay Kit 25 Competent E. coli TOP10 Cells 71 Complete Cell Fractionation Kit 26 CRG-2 ELISA Kit, mouse 154 CRMP1 / DRP-1 (CT) antibody 132 CRMP1 / DRP-1 (IN) antibody 132 CRP ELISA Kit, human 154 CRP, human, recombinant 171 CRTH2 (IN) antibody 94 CRTH2 (NT) antibody 94 CSN8 (CT) antibody 141 CTACK (CCL27), human, recombinant 171, 181 CTACK ELISA Kit, mouse 154 CTGF ( aa, His), human, recombinant 171 CTGF (98 aa), human, recombinant 171 CTGF (His), human, recombinant 171 CTGF antibody (mab) 94 CTGF antibody 94 CTGF EDK, human 163 CTGFL (WISP-2), human, recombinant 171 CTGFL / WISP-2 antibody 94 CTLA-4 antibody 94 CTRP1 / GIP (IN) antibody 94, 115 CTRP1 / GIP (NT) antibody 94, 115 CTRP2 (IN) antibody 94, 115 CTRP2 (NT) antibody 95, 115 CTRP2 antibody 95, 115 CTRP3 / CORS26 (IN) antibody 95, 115 CTRP4 (CT) antibody 95, 115 CTRP4 (NT) antibody 95, 115

203 Alphabetical Index 201 Description Page Description Page CTRP5 (IN) antibody 95, 115 CTRP5 (NT) antibody 95, 115 CTRP6 (CT) antibody 95, 115 CTRP6 (IN) antibody 95, 115 CTRP7 (CT) antibody 95, 115 CTRP7 (NT) antibody 95, 115 CTRP7 antibody 95, 115 Cu/ZnSOD ELISA Kit, human 162 CX3CL1 / CX3C antibody 136 CX3CR1 / V28 (EL) antibody 95,136 CX3CR1 / V28 (NT) antibody 95, 136 CXCL16 antibody 95 CXCL16 EDK, human 163 CXCL16 ELISA Kit, mouse 154 CXCL16, human, recombinant 171 CXCL16, mouse, recombinant 181 CXCR4 (EL) antibody 95, 136 CXCR4 (NT) antibody 95, 136 CXCR4 antibody 95, 136 CXCR5 antibody 95 Cycloheximide 44 Cycloheximide (100 mm) 44 Cyr61 antibody 95 CYR61, human, recombinant 171 Cytochrome c antibody (mab) 125 Cytochrome c Apoptosis Detection Kit 32 Cytochrome c ELISA Kit, human 162 Cytochrome p450 antibody 125 CytoRed Solution 14 Cytosol/Particulate Fractionation Kit 26 DAD1 antibody 125 Dansylaminoethyl-cyclen 21 DAP12 antibody 95 DAPI 13 DAPI Solution 13 DAPK2 antibody 125 DARC (CT) antibody 115 DARC (NT) antibody 115 DASPEI 13 Daunorubicin.HCl 44 Daxx / DAP-6 (CT) antibody 125 DC Marker antibody (mab) 115, 150 DcR1 / TRAIL-R3 (ED) antibody 125 DcR1 / TRAIL-R3 (ED2) antibody 126 DcR2 / TRAIL-R4 (ID) antibody 126 DcR3 / TR6 (NT) antibody 126 DcR3 ELISA Kit, human 162 DC-SIGN (CT) antibody 136 DC-SIGN (ED) antibody 136 DDX3 (IN) antibody 136 DDX3 (NT) antibody 136 D DEDAF antibody 126 DEDD2 / FLAME-3 antibody 126 Dengue type 2 antibody (mab) 139 Dexamethasone 44 Dexamethasone (10 mm) 44 DFF40 / CAD (I18) antibody 126 DFF40 / CAD (IN) antibody 126 DFF40 / CAD (NT) antibody 126 DFF45 / ICAD (CT) antibody 126 DFF45 / ICAD (NT) antibody 126 Di-12-ANEPPQ 16 Di-2-ANEPEQ (JPW1114) 16 Di-4-ANEPPS 16 Di-8-ANEPPQ 16 Di-8-ANEPPS 16 DiA 15 DiBAC 4 (3) 16 DiD (DiIC 18 [5]) 14 Dihydrorhodamine Dihydrorhodamine 123, dihydrochloride salt 13 DiI (or DiIC 18 (3)) 14 DiI in vegetable oil 14 DiIC 1 (5) 16 Dilinoleyl DiI 14 Dilinoleyl DiO 14 DiO (or DiOC 18 (3)) 14 DiOC 14 (3), hydroxyethanesulfonate 14 DiOC 2 (3) 16 DiOC 5 (3) 17 DiOC 6 (3) 13 DiOC 6 (3) 14 DiOC 6 (3) 17 DiR (DiIC18[7]) 14 DISC1 (IN) antibody 132 DISC1 (NT) antibody 132 D-JNK Peptide Inhibitor I 41 D-JNKI1 Negative Control Peptide 41 DKK-1 ELISA, human 163 D-Luciferin, Free Acid 12 D-Luciferin, Potassium Salt 12 D-Luciferin, Sodium Salt 12 DMNPE-caged D-Luciferin 12 DMSO, anhydrous 18, 22 DNA Damage Detection Kit 25 DNA Isolation Kit 27 DNase II (CT) antibody 126 Donkey Anti-Goat antibody, Fluorescein-coupled 151 Donkey Anti-Goat antibody, Texas Red-coupled 151 Doxorubicin.HCl 44 DPA/Terbium 17 DPX 17 DR3 / Apo-3 (CT) antibody 126 DR3 / Apo-3 (ED) antibody 126 DR3 / Apo-3 (NT) antibody 126

204 202 Alphabetical Index Description Page Description Page DR4 / TRAIL-R1 (CT) antibody 126 DR4 / TRAIL-R1 (NT) antibody 126 DR5 / TRAIL-R2 antibody 126 DR6 (NT) antibody 126 DRAK1 (NT) antibody 126 DRAK2 (CT) antibody 126 DRAM (CT) antibody 126 DRAM (NT) antibody 126 EB Solution 14 EBV (EBNA1) antibody (mab) 139 E-cadherin antibody (mab) 95 ECSIT (CT) antibody 140 EDC (EDAC) 22 EDN (EPX) ELISA Kit, human 163 EGF antibody (mab) 95 EGF antibody 95 EGF EDK, human 163 EGF EDK, mouse 163 EGF ELISA Kit, human 154 EGF, human, recombinant (E. coli) 171 EGF, human, recombinant (Pichia) 171 EGF, mouse, recombinant 181 EGF, rat, recombinant 184 EGF-R (active) ELISA Kit, human 154 EGFR antibody 95 EGF-R ELISA Kit, human 154 EG-VEGF antibody 96 EG-VEGF, human, recombinant 171 ELOVL6 antibody 149 EMAP-II antibody 96 EMAP-II, human, recombinant 171 Emerin antibody 136 ENA-78 antibody 96 ENA-78, human, recombinant 171 EndoG antibody 126 EndoGlyx-1 antibody (mab) 150 Endostatin antibody 96 Endostatin, human, recombinant 171 Endothelin 1/2 ELISA Kit, human 163 Eotaxin (CCL11) EDK, human 163 Eotaxin (CCL11), human, recombinant 171 Eotaxin (CCL11), mouse, recombinant 181 Eotaxin (CT) antibody 96, 136 Eotaxin antibody 96 Eotaxin ELISA Kit, mouse 154 Eotaxin-1 antibody (mab) 96 Eotaxin-2 (CCL24), human, recombinant 171 Eotaxin-2 (CCL24), mouse, recombinant 181 Eotaxin-2 antibody (mab) 96 Eotaxin-2 antibody 96 Eotaxin-2 ELISA Kit, human 154 E Eotaxin-3 (CCL26), human, recombinant 171 Eotaxin-3 antibody (mab) 96 Eotaxin-3 antibody 96 Eotaxin-3 EDK, human 164 EpCAM-1 antibody (mab) 150 Epigen, human, recombinant 171 Epiregulin, human, recombinant 171 EPO antibody (mab) 96 EPO ELISA Kit, human 154 EPO-alpha, human, recombinant 171 EPO-beta, human, recombinant 171 EPO-Receptor antibody (mab) 96 E-selectin (CD62E) ELISA Kit, human 155 E-selectin (CD62E) ELISA Kit, monkey 155 E-selectin antibody (mab) 96 E-selectin antibody (mab), biotin 96 E-selectin antibody (mab), FITC 96 E-selectin antibody 96 E-selectin, human, recombinant 171 Etoposide 44 Etoposide (10 mm) 44 EVER1 (IN) antibody 141 EVER1 (NT) antibody 141 EVER2 (IN) antibody 141 EVER2 (NT) antibody 142 Exodus-2 (CCL21), mouse, recombinant 181 Exodus-2 antibody 96 Exodus-2, human, recombinant 171 F1Aa (CT) antibody 126 FABP7 (IN) antibody 142 Factor-IX antibody (mab) 150 FAF1 (CT) antibody 126 FAIM antibody 127 FAP antibody (mab) 150 Fas / APO-1 ELISA Kit, human 162 Fas / CD95 antibody (mab) 127 Fas Ligand antibody (mab) 127 Fas Ligand antibody (mab), biotin 127 Fas Ligand antibody (mab), FITC 127 Fas Ligand ELISA Kit, human 162 Fas-1 / Apo1 antibody 127 FasL / Apo1L antibody 96 FastClone PCR Cloning Kit 1 66 FastClone PCR Cloning Kit 2 66 FastClone PCR Cloning Kit 3 66 FastExpress Gene Expression Kit 67 FastExpress Gene Expression Kit (HA) 67 FDA (fluorescein diacetate) 14 FEZ1 antibody 132 FEZ2 (IN) antibody 132 FEZ2 antibody 132 F

205 Alphabetical Index 203 Description Page Description Page FFP-18 AM 20 FGF-10 antibody 97 FGF-10, human, recombinant 172 FGF-16 antibody 97 FGF-16, human, recombinant 172 FGF-17 antibody 97 FGF-17, human, recombinant 172 FGF-18, human, recombinant 172 FGF-19 antibody 97 FGF-19, human, recombinant 172 FGF-20, human, recombinant 172 FGF-21, human, recombinant 172 FGF-22, human, recombinant 172 FGF-4 antibody 97 FGF-4 ELISA Kit, human 155 FGF-4, human, recombinant 172 FGF-5 antibody (mab) 97 FGF-5, human, recombinant 172 FGF-6 ELISA Kit, human 155 FGF-6, human, recombinant 172 FGF-8, human, recombinant 172 FGF-9 antibody 97 FGF-9 ELISA Kit, human 155 FGF-9, human, recombinant (Sf9) 172 FGF-9, mouse, recombinant 181 FGF-9, rat, recombinant 184 FGF-a (FGF-1), human, recombinant (E. coli) 171 FGF-a (FGF-1), human, recombinant (Sf9) 171 FGF-a (FGF-1), mouse, recombinant 181 FGF-a / FGF-1 antibody 96 FGF-b (FGF-2) ELISA Kit, human 155 FGF-b (FGF-2), human, recombinant 172 FGF-b (FGF-2), mouse, recombinant 181 FGF-b (FGF-2), rat, recombinant 184 FGF-b (FGF-2, 147 aa), human, recombinant 172 FGF-b / FGF-2 antibody (mab) 96 FGF-b / FGF-2 antibody 96 FGF-b (FGF-2) EDK, human 164 Fibronectin antibody 97 Fibronectin ELISA Kit, human 163 FIP-18 AM 20 FLAG-HRP antibody (mab) 148 FLAG-Tag antibody (mab) 148 FLASH (CT) antibody 127 FLIP / I-FLICE (CT) antibody 127 FLIP / I-FLICE (NT) antibody 127 FLIPs (CT) antibody 127 flt-3 Ligand antibody 97 flt-3 Ligand ELISA Kit, human 155 flt-3 Ligand, human, recombinant (E. coli) 172 flt-3 Ligand, human, recombinant (Sf9) 172 flt-3 Ligand, mouse, recombinant 181 Flubi-2 22 Flubida-2 22 Fluo-3 (pentaammonium salt) 19 Fluo-3 (pentapotassium salt) 19 Fluo-3 (pentasodium salt) 19 Fluo-3 AM 19 Fluo-3 AM Solution 19 Fluorescein Labeling Kit-NH Fluorescein-a-bungarotoxin 16 Fluorescein-Biotin 17 Fluorescein-DHPE 15 Fluorometric Cell Viability Kit I (Resazurin) 8 Fluorometric Cell Viability Kit II (Calcein) 8 Fluorometric DNA Fragmentation Detection Kit I (BrdU) 31 Fluorometric DNA Fragmentation Detection Kit II (BrdU) 31 Fluorometric DNA Fragmentation Detection Kit III (F-dUTP) 31 Fluorometric Glutathione Detection Kit 33 Fluorometric GST Assay Kit 25 Fluorometric Nitric Oxide Assay Kit 25 Fn14 / TNFRSF12 antibody 127 Follistatin antibody 97 Follistatin EDK, human 164 Fractalkine (CX3CL1), human, recombinant 172 Fractalkine (CX3CL1), rat, recombinant 184 Fractalkine antibody 97 Fractalkine ELISA Kit, human 155 Fractalkine ELISA Kit, rat 155 FST, human, recombinant 172 Fura-2 (pentaammonium salt) 19 Fura-2 (pentapotassium salt) 19 Fura-2 (pentasodium salt) 19 Fura-2 AM 19 Fura-2 AM Solution 19 Fura-2FF (K + salt) 20 Fura-2FF AM 20 Furaptra (tetrapotassium salt) 20 Furaptra (tetrasodium salt) 20 Furaptra, AM 20 gacrp30 (Adipolean), human, recombinant 169 gacrp30 (Adipolean), mouse, recombinant 181 gacrp30 / Adipolean antibody 97 Galectin-1 antibody 97 Galectin-1, human, recombinant 172 Galectin-2, human, recombinant 172 Galectin-3 antibody 97 Galectin-3 ELISA Kit, human 155, 163 Galectin-3, human, recombinant 172 Galectin-7 antibody 97 Galectin-9 antibody 97 GAPDH / G3PDH (CT) antibody 115 GAPDH / G3PDH (NT) antibody 115 GbL antibody 116 GCP-2 (CXCL6), human, recombinant 172 G

206 204 Alphabetical Index Description Page Description Page GCP-2 antibody 97 G-CSF antibody (mab) 97 G-CSF antibody 97 G-CSF EDK, human 164 G-CSF EDK, mouse 164 G-CSF ELISA Kit, human 155 G-CSF ELISA Kit, mouse 155 G-CSF, human, recombinant (CHO) 172 G-CSF, human, recombinant (E. coli) 172 G-CSF, mouse, recombinant 181 GDF-11, human, recombinant 173 GDF-15 (MIC-1), human, recombinant 173 GDF-3 antibody 97 GDF-3, human, recombinant 172 GDF-5, human, recombinant 172 GDF-8, human, recombinant 172 GDF-9 / BMP15 antibody 97 GDF-9, human, recombinant 173 GDNF antibody 97 GDNF, human, recombinant 173 GDNF, rat, recombinant 184 GeneBooster Reagent I 85 GeneBooster Reagent II 85 GeneBooster Reagent III 85 GeneBooster Reagent Kit 85 GeneOFF ppk-h1 shrna Vector Kit 73 GeneOFF ppk-h1-gfp shrna Vector Kit 73 GeneOFF ppk-u6 shrna Vector Kit 73 GeneOFF ppk-u6-gfp shrna Vector Kit 73 GeneOFF sirna Expression Kit (H1) 72 GeneOFF sirna Expression Kit (H1-GFP) 72 GeneOFF sirna Expression Kit (U6) 72 GeneOFF sirna Expression Kit (U6-GFP) 72 GeneOFF sirna Generation Kit 74 Genistein 44 Genomic DNA Isolation Kit I 27 Genomic DNA Isolation Kit II 27 GFP antibody (mab) 148 GFP antibody 148 GFP Reporter Assay Kit 11 GFRα-1 / GDNFRα (IN) antibody 97, 132 GFRα-2 / GDNFRβ (IN) antibody 97, 132 GFRα-3 / GDNFRγ (IN) antibody 97, 132 GH (Growth Hormone) antibody (mab) 97 GH (Growth Hormone) antibody 97 GH antibody (mab) 150 GH-20K-pit, human, recombinant 173 GH-20K-pl, human, recombinant 173 GH-22K-pl, human, recombinant 173 GHBP, human, recombinant 173 Ghrelin (CT) antibody 98 Ghrelin (NT) antibody 98 GITR ELISA Kit, human 155 GITRL / AITRL antibody 98, 115 Glutathione antibody (mab) 150 GM-CSF antibody (mab) 98 GM-CSF antibody 98 GM-CSF EDK, human 164 GM-CSF EDK, mouse 164 GM-CSF ELISA Kit, human 155 GM-CSF ELISA Kit, mouse 155 GM-CSF ELISA Kit, rat 155 GM-CSF, human, recombinant (E. coli) 173 GM-CSF, human, recombinant (Sf9) 173 GM-CSF, mouse, recombinant 181 GM-CSF, rat, recombinant 184 GM-CSF/IL-3 FP, human, recombinant (Sf9) 173 GMF-beta, human, recombinant 173 Goat Anti-Chicken antibody, Texas Red-coupled 151 Goat Anti-Mouse antibody, AP-coupled 151 Goat Anti-Mouse antibody, Biotin-coupled 151 Goat Anti-Mouse antibody, Fluorescein-coupled 151 Goat Anti-Mouse antibody, HRP-coupled 151 Goat Anti-Mouse antibody, Texas Red-coupled 151 Goat Anti-Rabbit antibody, Texas Red-coupled 151 Goat Anti-Rabbit antibody, AP-coupled 151 Goat Anti-Rabbit antibody, Biotin-coupled 151 Goat Anti-Rabbit antibody, Fluorescein-coupled 151 Goat Anti-Rabbit antibody, HRP-coupled 151 Goat Anti-Rat antibody, AP-coupled 151 Goat Anti-Rat antibody, HRP-coupled 151 Goat PF-XXX Anti-Human antibody 151 Goat PF-XXX Anti-Mouse antibody 151 Goat PF-XXX Anti-Rabbit antibody 151 GPAT1 antibody 149 GPR15 / BOB (NT) antibody 136 Granzyme A ELISA Kit, human 162 Granzyme B ELISA Kit, human 162 Granzyme B, mouse, recombinant 181 Green Caspase-12 Staining Kit 36 Green Caspase-2 Staining Kit 36 Green Caspase-3 Staining Kit 36 Green Caspase-8 Staining Kit 36 Green Caspase-9 Staining Kit 36 Green Multi-Caspase Staining Kit 36 Green-beta-D-Gal 12 Green-fluorescent Cytoplasmic Membrane Staining Kit 14 Grik1 (CT) antibody 132 Grik1 (IN) antibody 132 Grik2 (C2) antibody 132 Grik3 antibody 132 Grik4 (CT) antibody 132 Grik4 (NT) antibody 132 Grik5 antibody 133 GRO (KC, CXCL1) EDK, rat 164 GRO / KC antibody 98 GRO-alpha (KC, CXCL1), rat, recombinant 184 GRO-alpha (MGSA, CXCL1) EDK, human 164

207 Alphabetical Index 205 Description Page Description Page GRO-alpha (MGSA, CXCL1), human, recombinant 173 GRO-alpha / MGSA antibody 98 GRO-beta (MIP-2, CXCL2) EDK, human 164 GRO-beta (MIP-2, CXCL2), human, recombinant 173 GRO-beta (MIP-2, CXCL2), rat, recombinant 184 GRO-beta / MIP-2 antibody 98 GRO-beta antibody 98 GRO-gamma antibody 98 GRO-gamma (CXCL3), human, recombinant 173 Growth Hormone (GH), human, recombinant 173 Growth Hormone (GH), rat, recombinant 184 GRTP1 (NT) antibody 142 GSK-3a-GST Fusion Protein 41 GSK-3b antibody 116 GSK-3b, recombinant, human 41 GSK-3α antibody 115 GST Inhibitor-1 (Cibacron Blue F3G-A) 25 GST Inhibitor-2 (Ethacrynic acid) 25 GST protein (active) 25 GST-HRP antibody (mab) 148 GSTP1 antibody 142 GST-Tag antibody (mab) 148 GST-Tag antibody 148 H5N1 Hemagglutinin (IN) antibody 139 H5N1 Hemagglutinin (NT) antibody 139 H5N1 Neuramidase (CT) antibody 139 H5N1 Neuramidase (IN) antibody 139 H5N1 NS1 antibody 139 H5N1 NS2 / NEP antibody 139 HA-Biotin antibody (mab) 148 HA-FITC antibody (mab) 148 HA-HRP antibody (mab) 148 HAP1 antibody 133 HAT Activity Assay Kit 25 HAT protein (P/CAF, active) 25 HAT-1 antibody 127 HAT-2 antibody 127 HAT-3 antibody 127 HA-Tag antibody (mab) 148 HB-EGF, human, recombinant 173 HbsAgA antibody 139 HCAM Polyclonal Ab 127 HCC-1 (66 aa, CCL14), human, recombinant 173 HCC-1 (72 aa, CCL14), human, recombinant 173 HCC-1 antibody 98 HCC-4 ELISA Kit, human 155 hcg-beta Core antibody (mab) 150 HCVNS3 antibody (mab) 139 HDAC1 antibody 127 HDAC10 antibody 128 HDAC11 antibody 128 H HDAC2 antibody 127 HDAC3 antibody 127 HDAC4 antibody 127 HDAC5 antibody 127 HDAC6 antibody 127 HDAC7 antibody 127 HDAC8 antibody 127 HDAC9 antibody 127 HDV antibody (mab) 139 HEL antibody (mab) 150 HEP-1 / HPA-1 antibody (mab) 150 HEP-1 / HPA-1 antibody 149 HEP antibody (mab) 149 Hepatitis B antibody (mab) 139 HGF antibody 98 HGF ELISA Kit, human 155 HGF, human, recombinant 173 His-Tag antibody (mab) 148 His-Tag antibody 149 HIV-Igp120 (NT) antibody 136 HIV-Igp120 antibody (mab) 136 HIV-Igp120 antibody 136 HIV-Igp24 antibody (mab) 136 HIV-Igp41 antibody (mab) 136 HIV-Igp41 antibody 136 HIV-IIgp39 antibody (mab) 136 HIV-IIgp39 antibody 136 Hoechst Hoechst Solution 13 Hoechst Hoechst Solution 13 Hrk antibody 116 HVEM/Fc, human, recombinant 173 Hydroxystilbamidine 17 I-309 (CCL1), human, recombinant 173 I-309 antibody 98 ICAD / DFF45 (CT) antibody 128 ICAD / DFF45 (NT) antibody 128 ICAM-1 (scd54) ELISA Kit, human 155 ICAM-1 (scd54) ELISA Kit, monkey 155 ICAM-1 / CD54 antibody (mab) 98 ICAM-1 / CD54 antibody (mab), biotin 98 ICAM-1 / CD54 antibody (mab), FITC 98 ICAM-1 / CD54 antibody (mab) 98 ICAM-1 / CD 54 antibody 98 ICAM-1/ CD54 (NT) antibody (mab) 98 ICAM-2 (scd102) ELISA Kit, human 155 ICAM-2 / CD ICAM-2 / CD102 antibody (mab) 98 ICAM-2 / CD102 antibody (mab), FITC 99 ICAM-3 (scd50) ELISA Kit, human 155 I

208 206 Alphabetical Index Description Page Description Page ICAM-3 / CD50 antibody (mab) 99 IEX-1 antibody 128 IFN-alpha 1, human, recombinant 173 IFN-alpha 2a, human, recombinant 173 IFN-alpha 2b, human, recombinant (E. coli) 173 IFN-alpha 2b, human, recombinant (Yeast) 173 IFN-alpha antibody (mab) 99 IFN-alpha antibody 99 IFN-alpha ELISA Kit, human 155 IFN-beta (IN) antibody 99 IFN-beta 1a, human, recombinant (CHO) 173 IFN-beta 1b, human, recombinant 173 IFN-beta antibody (mab) 99 IFN-beta antibody 99 IFN-gamma antibody (mab) 99 IFN-gamma antibody (mab), biotin 99 IFN-gamma antibody (mab), FITC 99 IFN-gamma antibody 99 IFN-gamma EDK, human 164 IFN-gamma EDK, mouse 164 IFN-gamma EDK, rat 164 IFN-gamma ELISA Kit, human 155 IFN-gamma ELISA Kit, mouse 155 IFN-gamma ELISA Kit, pig 155 IFN-gamma ELISA Kit, rat 155 IFN-gamma, human, recombinant 173 IFN-gamma, mouse, recombinant 181 IFN-gamma, rat, recombinant 184 IFN-lambda-1 (IL-29), human, recombinant 173 IFN-lambda-2 (IL-28A), human, recombinant 174 IFN-lambda-2 (IL28A), mouse, recombinant 181 IFN-lambda-2 antibody 99 IFN-omega antibody (mab) 99 IFN-omega ELISA Kit, human 155 IgA (1&2) antibody (mab) 150 IgA (sec. comp.) antibody (mab) 150 IGF BP-3 antibody 99 IGF BP-5 antibody 100 IGF BP-7 antibody 100 IGF-BP1 ELISA Kit, human 155 IGF-BP1, human, recombinant 174 IGF-BP2 ELISA Kit, human 156 IGF-BP2, human, recombinant 174 IGF-BP3 ELISA Kit, human 156 IGF-BP3, human, recombinant 174 IGF-BP4 ELISA Kit, human 156 IGF-BP4, human, recombinant 174 IGF-BP5, human, recombinant 174 IGF-BP6 ELISA Kit, mouse 156 IGF-BP6, human, recombinant 174 IGF-BP7, human, recombinant 174 IGF-I antibody 100 IGF-I EDK, mouse 164 IGF-I ELISA Kit, human 156 IGF-I, human, recombinant 174 IGF-I, mouse, recombinant 181 IGF-I, rat, recombinant 184 IGF-II antibody 100 IGF-II, human, recombinant 174 IGF-IR, human, recombinant 174 IgG-Fc antibody (mab) 150 IgK antibody (mab) 150 IgL antibody (mab) 150 IKAP antibody 116 IKKα / IKK-1 (C1) antibody 116 IKKα / IKK-1 (C2) antibody 116 IKKα / IKK-1 (C3) antibody 116 IKKβ / IKK-2 (C3) antibody 116 IKKγ / NEMO / FIP3 (NT) antibody 116 IKKε / IKKi (CT) antibody 116 IL-1 alpha antibody (mab) 100 IL-1 alpha antibody 100 IL-1 alpha EDK, human 164 IL-1 alpha EDK, mouse 164 IL-1 alpha EDK, rat 164 IL-1 alpha ELISA Kit, human 156 IL-1 alpha ELISA Kit, mouse 156 IL-1 alpha ELISA Kit, rat 156 IL-1 alpha R1 antibody (mab) 100 IL-1 alpha R2 antibody (mab) 100 IL-1 alpha, human, recombinant 174 IL-1 alpha, mouse, recombinant 181 IL-1 alpha, rat, recombinant 184 IL-1 beta antibody (mab) 100 IL-1 beta antibody (mab), FITC 100 IL-1 beta antibody 100 IL-1 beta EDK, mouse 164 IL-1 beta EDK, rat 164 IL-1 beta ELISA Kit, human 156 IL-1 beta ELISA Kit, monkey 156 IL-1 beta ELISA Kit, mouse 156 IL-1 beta ELISA Kit, rat 156 IL-1 beta, human, recombinant 174 IL-1 beta, mouse, recombinant 181 IL-1 beta, rat, recombinant 184 IL-1 RA ELISA Kit, human 156 IL-1 RA, human, recombinant 174 IL-10 antibody (mab) 101 IL-10 antibody (mab), FITC 102 IL-10 antibody 102 IL-10 EDK, human 164 IL-10 EDK, mouse 164 IL-10 ELISA Kit, human 157 IL-10 ELISA Kit, monkey 157 IL-10 ELISA Kit, mouse 157 IL-10 ELISA Kit, rat 157 IL-10, human, recombinant (CHO) 175 IL-10, human, recombinant (E. coli) 175

209 Alphabetical Index 207 Description Page Description Page IL-10, mouse, recombinant 182 IL-10, rat, recombinant 185 IL-11 antibody (mab) 102 IL-11 antibody 102 IL-11 ELISA Kit, human 157 IL-11, human, recombinant 175 IL-11, mouse, recombinant 182 IL-12 (p40 + p70) ELISA Kit, mouse 157 IL-12 (p40) / IL-23 ELISA Kit, human 157 IL-12 (p70) ELISA Kit, human 157 IL-12 (p70) ELISA Kit, monkey 157 IL-12 (p70) ELISA Kit, mouse 157 IL-12 antibody (mab) 102 IL-12 antibody 102 IL-12 EDK, human 165 IL-12 EDK, mouse 165 IL-12, human, recombinant 175 IL-12, mouse, recombinant (CHO) 182 IL-12, mouse, recombinant (InC) 182 IL-12, rat, recombinant 185 IL-12-p40 antibody (mab) 102 IL-12-p40, human, recombinant (CHO) 175 IL-12-p40, mouse, recombinant (CHO) 182 IL-12-p75 antibody (mab) 102 IL-12-p80, human, recombinant (CHO) 175 IL-12-p80, mouse, recombinant (CHO) 182 IL-13 (Variant), human, recombinant 175 IL-13 antibody (mab) 102 IL-13 antibody (mab), FITC 102 IL-13 antibody 102 IL-13 beta, rat, recombinant 185 IL-13 EDK, human 165 IL-13 EDK, mouse 165 IL-13 ELISA Kit, human 157 IL-13 ELISA Kit, mouse 157 IL-13, human, recombinant 175 IL-13, mouse, recombinant 182 IL-13, rat, recombinant 185 IL-15 antibody (mab) 102 IL-15 antibody 102 IL-15 ELISA Kit, human 157 IL-15, human, recombinant 175 IL-15, mouse, recombinant 182 IL-15, rat, recombinant 185 IL-16 (121 aa), human, recombinant 175 IL-16 (130 aa), human, recombinant 175 IL-16 antibody 102 IL-16, mouse, recombinant 182 IL-17 antibody 102 IL-17 ELISA Kit, human 157 IL-17 ELISA Kit, mouse 157 IL-17, human, recombinant 175 IL-17, mouse, recombinant 182 IL-17, rat, recombinant 185 IL-17A EDK, human 165 IL-17B antibody 102 IL-17B, human, recombinant 175 IL-17D, human, recombinant 175 IL-17E antibody 102 IL-17E EDK, human 165 IL-17E, human, recombinant 175 IL-17E, rat, recombinant 185 IL-17F antibody 102 IL-17F, human, recombinant 175 IL-17F, mouse, recombinant 182 IL-18 antibody 102 IL-18 BP antibody 102 IL-18 ELISA Kit, pig 157 IL-18, rat, recombinant 185 IL-19 antibody 102 IL-19, human, recombinant 175 IL-1RAcP (C1) antibody 100, 116 IL-1RAcP (C2) antibody 100, 116 IL-2 antibody (mab) 100 IL-2 antibody 100 IL-2 CC, human, recombinant 174 IL-2 EDK, human 164 IL-2 EDK, mouse 164 IL-2 ELISA Kit, human 156 IL-2 ELISA Kit, monkey 156 IL-2 ELISA Kit, mouse 156 IL-2 ELISA Kit, rat 156 IL-2, human, recombinant 174 IL-2, mouse, recombinant 181 IL-2, rat, recombinant 184 IL-20 antibody 102 IL-20 EDK, human 165 IL-20, human, recombinant 175 IL-20, mouse, recombinant 182 IL-21 (IN) antibody 102 IL-21 antibody 102 IL-21 EDK, human 165 IL-21 ELISA Kit, human 157 IL-21, human, recombinant 175 IL-21, mouse, recombinant 182 IL-21R (ED) antibody 102 IL-21R (NT) antibody 103 IL-22 antibody 103 IL-22 EDK, human 165 IL-22 ELISA Kit, human 157 IL-22 ELISA Kit, mouse 157 IL-22, human, recombinant 175 IL-22, mouse, recombinant 182 IL-22R (CT) antibody 103 IL-22R (NT) antibody 103 IL-23 (CT) antibody 103 IL-23 (NT) antibody 103 IL-23 ELISA Kit, human 157

210 208 Alphabetical Index Description Page Description Page IL-24, human, recombinant 175 IL-25 (SF20), mouse, recombinant 182 IL-25 / SF20 antibody 103 IL-27 (IN) antibody 103 IL-27 (NT) antibody 103 IL-27 (p28), mouse, recombinant 182 IL-2R antibody (mab) 100 IL-2R ELISA Kit, human 156 IL-3 antibody (mab) 100 IL-3 antibody 100 IL-3 beta antibody 101 IL-3 beta, rat, recombinant 184 IL-3 CC, human, recombinant 174 IL-3 EDK, human 164 IL-3 EDK, mouse 164 IL-3, human, recombinant (E. coli) 174 IL-3, human, recombinant (Sf9) 174 IL-3, mouse, recombinant 182 IL-3, rat, recombinant 184 IL-31 (IN) antibody 103 IL-31 (NT) antibody 103 IL-31 antibody 103 IL-31 EDK, human 165 IL-31, human, recombinant 175 IL-31, mouse, recombinant 182 IL-32 (CT) antibody 103 IL-32 (IN) antibody 103 IL-32, human, recombinant 175 IL-33 (IN) antibody 103 IL-33 (NT) antibody 103 IL-33 antibody 103 IL-33, human, recombinant 175 IL-33, mouse, recombinant 182 IL-4 antibody (mab) 101 IL-4 antibody (mab), FITC 101 IL-4 antibody 101 IL-4 CC, human, recombinant 174 IL-4 EDK, human 164 IL-4 EDK, mouse 164 IL-4 ELISA Kit, human 156 IL-4 ELISA Kit, mouse 156 IL-4 ELISA Kit, rat 156 IL-4, human, recombinant (CHO) 174 IL-4, human, recombinant (E. coli) 174 IL-4, mouse, recombinant 182 IL-4, rat, recombinant 184 IL-5 antibody (mab) 101 IL-5 antibody 101 IL-5 EDK, human 164 IL-5 ELISA Kit, human 156 IL-5 ELISA Kit, mouse 156 IL-5, human, recombinant 174 IL-5, mouse, recombinant 182 IL-5, rat, recombinant 185 IL-6 (CHO), human, recombinant 174 IL-6 antibody (mab) 101 IL-6 antibody (mab), FITC 101 IL-6 antibody 101 IL-6 EDK, human 164 IL-6 EDK, mouse 164 IL-6 EDK, rat 164 IL-6 ELISA Kit, human 156 IL-6 ELISA Kit, monkey 156 IL-6 ELISA Kit, mouse 156 IL-6 ELISA Kit, rat 156 IL-6, human, recombinant 174 IL-6, human, recombinant (E. coli) 174 IL-6, mouse, recombinant 182 IL-6, rat, recombinant 185 IL-6R antibody (mab) 101 IL-6R antibody (mab), biotin 101 IL-6R ELISA Kit, human 156 IL-7 antibody (mab) 101 IL-7 antibody 101 IL-7 EDK, human 164 IL-7 ELISA Kit, human 156 IL-7 ELISA Kit, mouse 156 IL-7, human, recombinant (E. coli) 174 IL-7, human, recombinant (Yeast) 174 IL-7, mouse, recombinant 182 IL-7, rat, recombinant 185 IL-8 (CXCL8, 72 aa), human, recombinant 175 IL-8 (CXCL8, 77 aa), human, recombinant 175 IL-8 antibody (mab) 101 IL-8 antibody (mab), FITC 101 IL-8 antibody 101 IL-8 EDK, human 164 IL-8 ELISA Kit, human 156 IL-8 ELISA Kit, monkey 157 IL-9 antibody 101 IL-9 EDK, human 164 IL-9 ELISA Kit, mouse 157 IL-9, human, recombinant 175 IL-9, mouse, recombinant 182 ILP-2 antibody 128 ILPIP (CT) antibody 128 ILPIP (IN) antibody 128 Immunglobulin E [IgE] ELISA Kit, human 163 Immunglobulin G [IgG] ELISA Kit, human 163 Indo-1 (pentaammonium salt) 19 Indo-1 (pentapotassium salt) 19 Indo-1 (pentasodium salt) 19 Indo-1 AM 19 Indo-1FF (K + salt) 20 Indo-1FF AM 20 Influenza-A H1N1 antibody (mab) 138 Influenza-A H3N2 antibody (mab) 138 Influenza-A H5N1 antibody (mab) 138

211 Alphabetical Index 209 Description Page Description Page Influenza-B antibody (mab) 138 Insulin antibody (mab) 103 Insulin, human, recombinant 175 INT 8 Ionomycin (calcium salt) 22, 44 Ionomycin (free acid) 22 Ionomycin (free acid) 44 IP-10 (CXCL10), human, recombinant 175 IP-10 (CXCL10), mouse, recombinant 182 IP-10 (CXCL10), rat, recombinant 185 IP-10 antibody 103 IP-10 EDK, human 165 IP-10 ELISA Kit, human 157 IP-10 ELISA Kit, mouse 157 Ipaf / CARD12 antibody 128 IPR1 (CT) antibody 147 IPR1 (NT) antibody 147 IRAK (CT) antibody 116 IRAK-2 (C2) antibody 116 IRAK-2 (CT) antibody 116 IRAK-4 antibody 116 IRAK-M antibody 116 IRE1p / ERN1 (CT) antibody 116 IRE1p / ERN1 (IN) antibody 116 IRE1p / ERN1 (NT) antibody 116 IRF3 (CT) antibody 103 IRF3 (IN) antibody 103 IRF7 (CT) antibody 103 IRF7 (IN) antibody 103 IRF8 antibody 103 IRGM (IN) antibody 148 IRGM (NT) antibody 148 IRS-1 antibody 116 I-TAC (CXCL11), human, recombinant 175 I-TAC (CXCL11), mouse, recombinant 182 I-TAC antibody 103 I-TAC EDK, human 165 JC-1 chloride 13 JC-1 iodide 13 JNK Activated Cell Lysate 41 JNK Activity Detection Kit 41 JNK Activity Screening Kit 41 JNK Negative Control Cell Lysate 41 JNK1 antibody 116 KAI1 (CT) antibody 116 KappaB ras antibody 117 KappaB ras1 antibody 117 KappaB ras2 antibody 117 J K KC (GRO-alpha, CXCL1), mouse, recombinant 182 KGF (FGF-7), human, recombinant 175 KGF antibody 103 KGF-2, human, recombinant 175 KIF5 (CT) antibody 133 KIF5 (IN) antibody 133 KLHL1 antibody 133 LAG-1 (CCL4L1), human, recombinant 175 LAMP-1 antibody 128 LAMP-2 antibody 128 Lano antibody 103 LARC antibody 103 LD78 beta (CCL3L1), human, recombinant 175 LD78-beta antibody 104 LDH antibody 150 LDH Cytotoxicity Kit I 10 LDH Cytotoxicity Kit II 10 LEC (NCC-4), human, recombinant 175 LEC antibody (mab) 104 LEC antibody 104 Leptin antibody (mab) 104 Leptin antibody 104 Leptin EDK, mouse 165 Leptin ELISA Kit, human 157 Leptin ELISA Kit, mouse 157 Leptin Receptor antibody 104 Leptin, human, recombinant 175 Leptin, mouse, recombinant 182 Leptin, rat, recombinant 185 LeukinFeron, human, native 175 LFA-1 alpha / CD11a antibody (mab) 104 LFA-1 alpha / CD11a antibody (mab), biotin 104 LFA-1 alpha / CD11a antibody (mab), FITC 104 LFA-1 beta / CD18 antibody (mab) 104 LFA-1 beta / CD18 antibody (mab), biotin 104 LFA-1 beta / CD18 antibody (mab), FITC 104 LFA-3, human, recombinant 176 LFG antibody 128 LGI1 (IN) antibody 133 LGI1 (NT) antibody 133 LGI2 (IN) antibody 133 LGI2 (NT) antibody 133 LGI3 (IN) antibody 133 LGI4 (CT) antibody 133 LGI4 (IN) antibody 133 LGP2 (CT) antibody 147 LGP2 (IN) antibody 147 LGP2 (NT) antibody 147 LIF ELISA Kit, human 157 LIF-R (gp190) ELISA Kit, human 157 LIGHT antibody 104 L

212 210 Alphabetical Index Description Page Description Page LIGHT ELISA Kit, human 157 LIGHT, human, recombinant 176 LIGHT, mouse, recombinant 182 LIMP2 antibody 142 Lipe / HSL antibody 149 LIS1 antibody 133 Live/Dead Cell Staining Kit I 9 Live/Dead Cell Staining Kit II 9 Livin / KIAP antibody 128 LIX (CXCL5, 70 aa), mouse, recombinant 182 LIX (CXCL5, 93 aa), mouse, recombinant 182 LIX antibody 104 LIX ELISA Kit, rat 157 L-JNK Peptide Inhibitor I 41 L-JNKI1 Negative Control Peptide 41 LKB1 antibody 117 LSD1 / AOF2 (IN) antibody 142 LSD1 / AOF2 (NT) antibody 142 L-selectin antibody (mab) 104 L-selectin antibody (mab), biotin 104 L-selectin antibody (mab), FITC 104 L-selectin antibody 104 L-selectin ELISA Kit, human 157 L-selectin, human, recombinant 176 Lucifer Yellow Cadaverine 17 Lucifer Yellow Cadaverine Biotin-X, dipotassium salt 17 Lucifer Yellow CH lithium salt 17 Lucifer Yellow CH potassium salt 17 Luciferase antibody 150 Luciferase Reporter Assay Kit I (Firefly) 11 Luciferase Reporter Assay Kit II (Renilla) 11 Luciferase Reporter Assay Kit III (Firefly & Renilla) 11 Luciferase Reporter HTS Kit (Firefly) 11 Lungkine (CXCL15), mouse, recombinant 182 Lymphotactin antibody 104 Lymphotactin, human, recombinant 176 M MA Lipofection Enhancer 84 Mac-1 alpha / CD11b antibody (mab) 104 Mac-1 alpha / CD11b antibody (mab), biotin 105 Mac-1 alpha / CD11b antibody (mab), FITC 105 Macrophages antibody (mab) 150 MadCAM-1 antibody (mab) 150 MADD / DENN (CT) antibody 117 Magnet Plate 84 Magnetic Separator (re-usable) 43 Mammalian Whole Cell Protein Extraction Kit 26 MANF (CT) antibody 133 MANF (NT) antibody 133 MAP1 (IN) antibody 128 MAPKAP1 (IN) antibody 117 MAPKAP1 (NT) antibody 117 Maspin, human, recombinant 176 MATra-A Reagent 84 MATra-S Immobilizer 84 MATra-siRNA Reagent 84 MBP antibody (mab) 149 MCAF / MCP-1 antibody (mab) 105 MCG10 antibody 128 Mcl-1 (CT) antibody 128 Mcl-1 (IN) antibody 128 Mcl-1 (NT) antibody 128 MCP-1 (CCL2) EDK, rat 165 MCP-1 (CCL2), rat, recombinant 185 MCP-1 (JE, CCL2) EDK, mouse 165 MCP-1 (JE, CCL2), mouse, recombinant 182 MCP-1 (MCAF, CCL2) EDK, human 165 MCP-1 (MCAF, CCL2), human, recombinant 176 MCP-1 antibody 105 MCP-1 ELISA Kit, human 157 MCP-1 ELISA Kit, mouse 157 MCP-1 ELISA Kit, rat 157 MCP-2 (CCL8), human, recombinant 176 MCP-2 (CCL8), mouse, recombinant 182 MCP-2 antibody (mab) 105 MCP-2 antibody 105 MCP-2 ELISA Kit, human 158 MCP-3 (CCL7) EDK, mouse 165 MCP-3 (CCL7), human, recombinant 176 MCP-3 (CCL7), mouse, recombinant 183 MCP-3 antibody (mab) 105 MCP-3 antibody 105 MCP-4 (CCL13), human, recombinant 176 MCP-4 antibody (mab) 105 MCP-4 antibody 105 MCP-5 (CCL12), mouse, recombinant 183 MCP-5 antibody 105 MCP-5 ELISA Kit, mouse 158 M-CSF antibody 105 M-CSF EDK, mouse 165 M-CSF ELISA Kit, human 158 M-CSF, human, recombinant 176 M-CSF, mouse, recombinant 183 M-CSF, rat, recombinant 185 MD-1 (CT) antibody 105 MD-1 (IN) antibody 140 MD-2 (IN) antibody 140 MDA5 (CT) antibody 147 MDA5 (IN) antibody 147 MDC (CCL22) EDK, mouse 165 MDC (CCL22), mouse, recombinant 183 MDC (CCL22, 67 aa), human, recombinant 176 MDC (CCL22, 69 aa), human, recombinant 176 MDC antibody (mab) 105 MDC antibody 105 MDC ELISA Kit, human 158

213 Alphabetical Index 211 Description Page Description Page MEC (CCL28), human, recombinant 176 MEC (CCL28), mouse, recombinant 183 Megalin / LRP2 antibody (mab) 150 Membrane Protein Extraction Kit 26 MGP ELISA Kit, human 163 MIA antibody 105 MIA, human, recombinant 176 MIA-2 antibody 105 MIA-2, human, recombinant 176 MICA antibody 147 Microglobulin ELISA Kit, human 163 Midkine (MK) EDK, human 165 Midkine antibody 105 Midkine, human, recombinant 176 MIF antibody 105 MIF, human, recombinant 176 MIG (CXCL9), human, recombinant 176 MIG (CXCL9), mouse, recombinant 183 MIG antibody (mab) 105 MIG antibody 105 MIG ELISA Kit, human 158 MIP-1 alpha (CCL3) EDK, human 165 MIP-1 alpha (CCL3) EDK, mouse 165 MIP-1 alpha (CCL3), human, recombinant 176 MIP-1 alpha (CCL3), mouse, recombinant 183 MIP-1 alpha (CCL3), rat, recombinant 185 MIP-1 alpha (CCL3) EDK, rat 165 MIP-1 alpha antibody (mab) 105 MIP-1 alpha antibody 106 MIP-1 alpha ELISA Kit, mouse 158 MIP-1 beta (CCL4), human, recombinant 176 MIP-1 beta (CCL4), mouse, recombinant 183 MIP-1 beta (CCL4), rat, recombinant 185 MIP-1 beta antibody 106 MIP-1 beta ELISA Kit, human 158 MIP-1 delta ELISA Kit, human 158 MIP-1 gamma (CCL9/10), mouse, recombinant 183 MIP-1 gamma antibody 106 MIP-2 (GRO-beta, CXCL2) EDK, mouse 165 MIP-2 (GRO-beta, CXCL2), mouse, recombinant 183 MIP-2 antibody 106 MIP-2 ELISA Kit, mouse 158 MIP-3 (CCL23), human, recombinant 176 MIP-3 alpha (CCL20), human, recombinant 176 MIP-3 alpha (CCL20), mouse, recombinant 183 MIP-3 alpha antibody (mab) 106 MIP-3 alpha antibody 106 MIP-3 alpha ELISA Kit, human 158 MIP-3 antibody (mab) 106 MIP-3 antibody 106 MIP-3 beta (CCL19), human, recombinant 176 MIP-3 beta (CCL19), mouse, recombinant 183 MIP-3 beta antibody (mab) 106 MIP-3 beta antibody 106 MIP-4 (CCL18), human, recombinant 176 MIP-4 antibody 106 MIP-5 (CCL15), human, recombinant 176 MIP-5 antibody 106 MitoCapture Incubation Buffer 32 Mitochondria/Cytosol Fractionation Kit 26 Mitochondrial Apoptosis Staining Kit 32 Mitochondrial DNA Isolation Kit 27 Mitomycin C 44 MitoRed 13 MMP-1 ELISA Kit, human 158 MMP-10 antibody (mab) 106 MMP-10 ELISA Kit, human 158 MMP-11 antibody 107 MMP-12 antibody (mab) 107 MMP-12 antibody 107 MMP-13 antibody (mab) 107 MMP-13 antibody 107 MMP-13 ELISA Kit, human 158 MMP-14 antibody (mab) 107 MMP-15 antibody (mab) 107 MMP-17 antibody 107 MMP-19 antibody 107 MMP-2 antibody 106 MMP-24 antibody 107 MMP-25 antibody (mab) 107 MMP-3 antibody (mab) 106 MMP-3 antibody 106 MMP-3 ELISA Kit, human 158 MMP-3, human, recombinant 176 MMP-7 antibody (mab) 106 MMP-7, human, recombinant 177 MMP-8 antibody (mab) 106 MMP-8 antibody 106 MMP-8 ELISA Kit, human 158 MMP-9 antibody 106 MMP-9 ELISA Kit, human 158 MnSOD antibody (mab) 145 MnSOD antibody (mab), FITC 145 MOG antibody (mab) 150 MORF4 antibody 142 Mpg-1 antibody 107 MPO antibody 150 MPO ELISA Kit, human 158 MQAE 21 MSP ELISA Kit, human 158 MTBP (IN2) antibody 131 MTT 8 Myc-HRP antibody (mab) 149 Mycoplasma-EX 191 Mycoplasma-EX Spray 193 Myc-Tag antibody (mab) 149 Myc-Tag antibody 149 MyD88 (CT) antibody 117

214 212 Alphabetical Index Description Page Description Page MyD88 (IN) antibody 117 Myeloperoxidase (MPO) antibody 107 Myostatin ELISA Kit, human 158 Myostatin, human, recombinant 177 Nicastrin (CT) antibody 133 Nicastrin (IN) antibody 133 NIK (CT) antibody 133 NIPSNAP (CT) antibody 133 NIPSNAP (IN) antibody 133 NAD/NADH Quantitation Kit 25 NADE antibody 128 NADP/NADPH Quantitation Kit 25 NAIP (CT) antibody 128 NAK / TBK1 (CT) antibody 117 NALP1 / CARD7 / DEFCAP antibody 128 NALP2 / PYPAF2 antibody 128 Nanog antibody 107 Nanog, human, recombinant 177 Nanos1 (IN) antibody 117 Nanos1 (NT) antibody 117 Nanos2 (CT) antibody 117 Nanos2 (NT) antibody 117 Nanos3 (CT) antibody 117 NAP-2 (CXCL7) EDK, human 165 NAP-2 (CXCL7), human, recombinant 177 NAP-2 antibody (mab) 107 NAP-2 antibody 107 NAP-2 ELISA Kit, human 158 NBD C6-ceramide 15 NBD C6-sphingomyelin 15 NBD-DHPE 15 Nephrin antibody 142 Nerve Terminal Green Staining Kit I 15 Nerve Terminal Green Staining Kit II 15 Nerve Terminal Green Staining Kit III 15 Nerve Terminal Green Staining Kit IV 15 Nerve Terminal Red Staining Kit I 15 Nerve Terminal Red Staining Kit II 15 Neuregulin-1 (NRG-1), human, recombinant 177 Neuritin antibody 133 Neuro-DiI 15 Neuro-DiI in vegetable oil 15 Neuro-DiO 15 Neuro-DiO in vegetable oil 15 Neuroglobin, human, recombinant 177 Neuropoietin (NP), mouse, recombinant 183 Neuroserpin antibody 107 Neuroserpin, human, recombinant 177 Neurotrypsin (NT) antibody 133 Neurturin (NTN), human, recombinant 177 Neurturin antibody 107, 133 Neutrophil antibody (mab) 107 NG antibody (mab) 149 NGFR / TNFRSF16 antibody 107, 117 Nhe-1 (CT) antibody 133 Nhe-1 (IN) antibody 133 N NK3R (IN) antibody 133 NNT-1 (BCSF-3), human, recombinant 177 NNT-1 / BCSF-3 antibody 107 NOD2 / CARD15 (CT) antibody 128 NOD2 / CARD15 (NT) antibody 128 NOGGIN, human, recombinant 177 NOGGIN, mouse, recombinant 183 NogoA (IN) antibody 134 NogoA (NT) antibody 134 Nonyl Acridine Orange (or NAO) 13 Nop30 antibody 128 NOV antibody 107 NOV EDK, human 165 NOV, human, recombinant 177 Noxa / PMAIP1 / APR antibody 128 NP-1 antibody 107 NP-1, human, recombinant 177 NPAS3 (IN) antibody 134 NPAS3 (NT) antibody 134 NPTX2 antibody 134 NT-3 antibody 107 NT-3 ELISA Kit, human 158 NT-3, human, recombinant 177 NT-4 antibody (mab) 107 NT-4 antibody 107 NT-4, human, recombinant 177 NTB 8 NT-proANP 1-98 ELISA Kit, human 163 NT-proCNP ELISA Kit, human 163 Nuclear/Cytosol Fractionation Kit 26 Nudel antibody 117 Octadecyl Rhodamine B Chloride (R18) 15 Okadaic acid 44 Omentin, human, recombinant 177 OMI / Htr2A antibody 129 OMI / HtrA2 (C2) antibody 129 Oncostatin M antibody 108 Oncostatin M, human, recombinant 177 op18 (CT) antibody 142 op18 (NT) antibody 142 OPG (OCIF), human, recombinant 177 OPG antibody 108 OPG ELISA Kit, human 158, 162 OptiCells Competent E. coli Cells 66 OptiCells Competent E. coli F' Cells 66 ORAI1 (CT) antibody 147 O

215 Alphabetical Index 213 Description Page Description Page ORAI1 (NT) antibody 147 ORAI2 (CT) antibody 147 ORAI3 (CT) antibody 147 ORAI3 (NT) antibody 147 Orange-fluorescent Cytoplasmic Membrane Staining Kit 14 OTOR, human, recombinant 177 OX-40 (CD134) ELISA Kit, human 162 OX40L, human, recombinant 177 Oxidized Glutathione (GSSG) 25 Oxidized LDL (oxldl) ELISA Kit, human 163 p185 (HER-2) ELISA Kit, human 158 p185(her-2) antibody (mab) 145 p185(her-2) antibody (mab), biotin 145 p185(her-2) antibody (mab), FITC 145 p53 antibody (mab) 131 p53 antibody 131 p53 ELISA Kit, human 163 p53aip1 antibody 136 p53dinp1 / SIP antibody 131 p53r2 (NT) antibody 131 p62dok (CT) antibody 117 PAF-AH, human, recombinant 177 PAI-1 antibody 108 PAI-1 EDK, human 165 PAI-1 ELISA Kit, human 158 PAI-2, human, recombinant 177 PAK2 (CT) antibody 117 PAK2 (NT) antibody 117 PAK4 antibody 117 PAK5 antibody 117 PAK6 antibody 117 PAP-1 antibody 147 PARC antibody 131 PARC ELISA Kit, human 158 PCR Bacteria Test Kit 192 PCR Mycoplasma Test Kit I/C 190 PCR Mycoplasma Test Kit I/RT 190 PCR Mycoplasma Test Kit II 190 PDCD1 (CT) antibody 129 PDCD1 (IN) antibody 129 PDCD4 (CT) antibody 129 PDGF-AA antibody (mab) 108 PDGF-AA antibody 108 PDGF-AA, human, recombinant 177 PDGF-AB, human, recombinant 177 PDGF-BB antibody (mab) 108 PDGF-BB antibody 108 PDGF-BB EDK, human 165 PDGF-BB ELISA Kit, human 158 PDGF-BB, human, recombinant (E. coli) 177 PDGF-BB, human, recombinant (Yeast) 177 P PDGF-BB, mouse, recombinant 183 PDGF-CC, human, recombinant 177 PDGFR-alpha antibody (mab) 108 PDGFR-beta antibody (mab) 108 PDL-1 antibody 129 PDL-2 antibody 129 PECAM-1 (scd31) ELISA Kit, human 158 PECAM-1 (scd31) ELISA Kit, monkey 158 PECAM-1 antibody (mab) 108 PECAM-1 antibody (mab), biotin 108 PECAM-1 antibody (mab), FITC 108 PEDF, human, recombinant 177 PEN2 (CT) antibody 134 PEN2 (NT) antibody 134 Periostin, human, recombinant 177 Peroxidase Labeling Kit-NH Peroxidase Labeling Kit-SH 152 PERP antibody 131 Persephin antibody 108 Persephin, human, recombinant 177 Persephin, mouse, recombinant 183 PF-4 (CXCL4), human, recombinant 177 PF-4 antibody 108 PF-XXX-aaddUTP 54 PF-XXX-aadUTP 54 PF-XXX-aaUTP 54 PF-XXX-Avidin Conjugate 53 PF-XXX-Biotin Conjugate 53 PF-XXX-Goat anti-human IgG 52 PF-XXX-Goat anti-mouse IgG 52 PF-XXX-Goat anti-rabbit IgG 52 PF-XXX-Labeling-Kit 50, 152 PF-XXX-Phalloidin 52 PF-XXX-Rabbit anti-fluorescein conj. 52 PF-XXX-Streptavidin Conjugate 53 PGRN, human, recombinant 178 PHAP (CT) antibody 129 PHAP (NT) antibody 129 PHAP I (CT) antibody 129 PHAP I (IN) antibody 129 PHAP III antibody 129 Pharmacidal Spray 193 Phorbol-12-myristate 13-acetate (PMA) 44 Phospho-AFX antibody 145 Phospho-Akt antibody 145 Phospho-ATF-2 antibody 145 Phospho-Bad antibody 145 Phospho-b-Catenin antibody 145 Phospho-BRCA1 (Ser1189) antibody 145 Phospho-CaMKII P antibody 145 Phospho-c-Jun (Ser73) antibody 145 Phospho-c-Jun antibody 145 Phospho-c-Myc antibody 145 Phospho-DARPP-32 antibody 145

216 214 Alphabetical Index Description Page Description Page Phospho-Elk-1 antibody 145 Phospho-Erk1/2 antibody 145 Phospho-FAK antibody 145 Phospho-GSK-3a antibody 145 Phospho-GSK-3b antibody 145 Phospho-JKK/SEK1/MKK4 antibody 145 Phospho-JNK/SAPK (Thr183/Tyr185) antibody 146 Phospho-Lck antibody 146 Phospho-MAPKAPK-2 antibody 146 Phospho-MARCKS antibody 146 Phospho-Mek1/2 antibody 146 Phospho-MLK3 antibody 146 Phospho-MSK1 antibody 146 Phospho-p38 MAPK antibody 146 Phospho-p53 (Ser15) antibody 146 Phospho-p70 S6 Kinase (Thr389) antibody 146 Phospho-PKC antibody 146 Phospho-Raf (Ser259) antibody 146 Phospho-Stat1 antibody 146 Phospho-Stat2 antibody 146 Phospho-Stat3 antibody 146 Phospho-Stat5 antibody 146 Phospho-Stat6 antibody 146 Phospho-Tau antibody 146 Phospho-TrkA (Tyr490) antibody 146 Phospho-Tyrosine Hydroxylase antibody 146 Phospho-VASP antibody 146 PID / MTA2 antibody 131 PIKE (CT) antibody 129 PIST / GOPC (CT) antibody 129 PIST / GOPC (IN) antibody 129 PKR (CT) antibody 108 PKR (NT) antibody 108 Plant DNA Isolation Buffer 27 Plectin antibody (mab) 150 Plectin antibody (mab), biotin 150 Pleiotrophin, human, recombinant 177 PLGF antibody (mab) 108 PLGF-1 antibody 108 PLGF-1 EDK, human 166 PLGF-1, human, recombinant (E. coli) 177 PLGF-1, human, recombinant (Sf9) 177 PLGF-2, human, recombinant (E. coli) 178 PLGF-2, human, recombinant (Sf9) 178 PLGF-2, mouse, recombinant 183 PLP antibody (mab) 150 Pluronic F Pluronic F-127 (sample size) 22 Pluronic F-127, 10% in H 2 O 18, 22 Pluronic F-127, 20% solution in DMSO 18, 22 Plxdc2 (CT) antibody 142 Plxdc2 (NT) antibody 142 PMN-Elastase ELISA Kit, human 158 PMS 8 Podoplanin antibody (mab) 108, 145 Podoplanin antibody 108 ppk-cmv-e1 63 ppk-cmv-e2 63 ppk-cmv-e3 63 ppk-cmv-f1 (C-GFP) 65 ppk-cmv-f2 (N-GFP) 65 ppk-cmv-f3 (C-Luc) 65 ppk-cmv-f4 (N-Luc) 65 ppk-cmv-r0 (Blank) 64 ppk-cmv-r1 (b-gal) 64 ppk-cmv-r2 (CAT) 64 ppk-cmv-r3 (Luc) 64 ppk-cmv-r4 (GFP) 64 ppk-cmv-r5 (SEAP) 64 Precerebellin / Cbln1 (CT) antibody 117 Precerebellin / Cbln1 (IN) antibody 117 Presenilin 1 antibody 108 Presenilin 2 antibody 108 Presenilin-1 antibody 134 ProBMP-2, human, recombinant 178 Prokinectin-2, human, recombinant 178 Prolactin Receptor (Prlr), human, recombinant 178 Prolactin, human, recombinant 178 Prolactin, mouse, recombinant 183 Prolactin, rat, recombinant 185 PromoFectin 80 PromoFectin-CellLine 80 PromoFectin-Hepatocyte 80 PromoFectin-HUVEC 80 PromoFectin-Insect 80 PromoFectin-Neuron 80 PromoFectin-Oligo 80 PromoFectin-Polypeptide 80 PromoFectin-siRNA 80 PromoFluor Amino-Modified Label 49 PromoFluor Antifade Reagent 59 PromoFluor Carboxylic Acid 49 PromoFluor ChipPack 49 PromoFluor Maleimide 49 PromoFluor NHS-Ester 49 PromoFluor-430 Nick Translation Labeling Kit 51 PromoFluor-430 PCR Labeling Kit 51 PromoFluor-500 Nick Translation Labeling Kit 51 PromoFluor-500 PCR Labeling Kit 51 PromoFluor-550 Nick Translation Labeling Kit 51 PromoFluor-550 PCR Labeling Kit 51 PromoFluor-594 Nick Translation Labeling Kit 51 PromoFluor-594 PCR Labeling Kit 51 PromoFluor-640 Nick Translation Labeling Kit 51 PromoFluor-640 PCR Labeling Kit 51 PromoKine ECL 153 PromoKine Hybridization Solution 153 PromoKine Super-Block 153

217 Alphabetical Index 215 Description Page Description Page Pro-NGF, human, recombinant 178 Propidium Iodide (PI) 14 Propidium Iodide (PI) Solution 14 Prox-1 (fragment), human, recombinant 178 Prox-1 antibody 108 P-selectin antibody 108 P-selectin ELISA Kit, human 159 P-selectin ELISA Kit, mouse 159 P-selectin, human, recombinant 178 PSGL-1 antibody (mab) 108 PTEN / MMAC / TEP-1 (CT) antibody 118 PTEN / MMAC / TEP-1 (NT) antibody 118 PTHrP, human, recombinant 178 PTK7 (CT) antibody 142 PTK7 (NT) antibody 142 PUMA / bbc3 (CT) antibody 129 PUMA / bbc3 (NT) antibody 129 Purple-beta-D-Gal 12 Q Quin-2 (K + salt) 19 Rabbit Anti-Chicken antibody, AP-coupled 152 Rabbit Anti-Chicken antibody, HRP-coupled 152 Rabbit Anti-Goat antibody, AP-coupled 152 Rabbit Anti-Goat antibody, HRP-coupled 152 Rabbit Anti-Mouse antibody, HRP-coupled 152 Rabbit PF-XXX Anti-Fluorescein antibody 152 RAIDD / CRADD (CT) antibody 129 RAIDD / CRADD (IN) antibody 129 RANTES (CCL5) EDK, human 166 RANTES (CCL5) EDK, mouse 166 RANTES (CCL5) EDK, rat 166 RANTES (CCL5), human, recombinant 178 RANTES (CCL5), mouse, recombinant 183 RANTES (CCL5), rat, recombinant 185 RANTES antibody (mab) 109 RANTES antibody 109 RANTES ELISA Kit, human 159 RAP, rat, recombinant 185 RAP80 (CT) antibody 142 RAP80 (NT) antibody 142 Rapamycin 44 Raptor (CT) antibody 118 Raptor (IN) antibody 118 Raptor (NT) antibody 118 RBC Lysis Solution 27 RBP ELISA Kit, human 163 Red Caspase-3 Staining Kit 36 Red Caspase-8 Staining Kit 36 Red Caspase-9 Staining Kit 36 R Red Multi-Caspase Staining Kit 36 Red-beta-D-Gal 12 Red-fluorescent Cytoplasmic Membrane Staining Kit 14 Reduced Glutathione (GSSG) 25 Relaxin ELISA Kit, human 161 Relaxin-2, human, recombinant 178 Relaxin-3, human, recombinant 178 RELM beta, human, recombinant 178 RELM-alpha antibody 109 RELM-alpha, mouse, recombinant 183 RELM-beta antibody 109 RELM-beta, mouse, recombinant 183 RELM-gamma, mouse, recombinant 183 Resazurin, sodium salt 8 Resistin antibody (mab) 109 Resistin antibody 109 Resistin EDK, human 166 Resistin ELISA Kit, human 159 Resistin, human, recombinant 178 Resistin, mouse, recombinant 183 Resistin, rat, recombinant 185 RFP antibody (mab) 149 RFP antibody 149 RGP1 (NT) antibody 142 RGPD5 (NT) antibody 142 RH RH RH RH Rheb (IN) antibody 118 Rheb (NT) antibody 118 Rhod-2 (triammonium salt) 19 Rhod-2 (tripotassium salt) 20 Rhod-2 (trisodium salt) 20 Rhod-2 AM 20 Rhodamine 110 Labeling Kit-NH Rhodamine 110 Labeling Kit-SH 152 Rhodamine Rhodamine-DHPE 15 rhu Calpain I 40 rhu Cathepsin D 40 rhu Cathepsin L 40 rhu GDNF, mouse, recombinant 181 RICK / RIP2 (CT) antibody 129 RICK / RIP2 (NT) antibody 129 Rift Valley Fever Virus (CT) antibody 139 Rift Valley Fever Virus (IN) antibody 139 RIG-1 antibody 140 Rim2 antibody 134 Rim3 (NT) antibody 134 RIP3 antibody 129 Rose-beta-D-Gal 12 Rosiglitazone 44 RP105 (CT) antibody 109

218 216 Alphabetical Index Description Page Description Page RP105 (IN) antibody 109 RP105 (NT) antibody 109 RPA Interacting Protein (CT) antibody 142 RPA Interacting Protein (NT) antibody 142 R-Phycoerythrin Labeling Kit-NH 2 57, 153 R-Phycoerythrin Labeling Kit-SH 57, 153 R-Phycoerythrin, lyophilized 56 RPS6K1 / p70-s6k antibody 118 RTP801 (IN) antibody 142 RTP801 (NT) antibody 142 RuBP-2S 17 RuBP-4S 17 RuBPS 17 scd30 ELISA Kit, human 160 scd31 (specam-1) ELISA Kit, human 160 scd31 (specam-1) ELISA Kit, monkey 160 scd4, human, recombinant 170 scd4, human, recombinant 170 scd40 ELISA Kit, human 160 scd40 Ligand (CD154), human, recombinant 170 scd40 Ligand, mouse, recombinant 181 scd40l EDK, human 163 scd44std ELISA Kit, human 160 scd44var (v5) ELISA Kit, human 161 scd44var (v6) ELISA Kit, human 161 scd50 (sicam-3) ELISA Kit, human 161 scd54 (sicam-1) ELISA Kit, human 161 SAPAP1 antibody 134 SAPAP2 antibody 134 SAPAP3 antibody 134 SARM (CT) antibody 140 SARS E (CT) antibody 137 SARS E (NT) antibody 137 SARS M (CT) antibody 137 SARS M (NT) antibody 137 SARS Spike (CT) antibody 137 SARS Spike (IN1) antibody 137 SARS Spike (IN2) antibody 137 SARS Spike (IN3) antibody 137 SARS Spike (NT) antibody 137 SARS Spike antibody (mab) 137 SARS Spike antibody 137 SARS-NP antibody (mab) 138 SARS-NP antibody 138 SATB1 (CT) antibody 118 SATB1 (NT) antibody 118 SATB2 (CT) antibody 118 SATB2 (NT) antibody 118 sbmpr-1a, human, recombinant (Sf9) 170 SCAS 16 scd102 (sicam-2) ELISA Kit, human 161 scd105 (Endoglin) antibody (mab) 94 scd105 (Endoglin), human, recombinant (Sf9) 170 scd105 (Endoglin), mouse, recombinant (Sf9) 181 scd105 / Endoglin antibody 94 scd106 (svcam-1) ELISA Kit, human 161 scd134 (OX-40) ELISA Kit, human 161 scd137 (4-1BB) ELISA Kit, human 161 scd144 (sve-cadherin) ELISA Kit, human 161 scd152 (sctla-4) ELISA Kit, human 161 scd153 (scd30l) ELISA Kit, human 161 scd162 (spsgl-1) ELISA Kit, human 161 scd23 ELISA Kit, human 160 scd26 ELISA Kit, human 160 scd28 ELISA Kit, human 160 S scd54 (sicam-1) ELISA Kit, monkey 161 scd62e (se-selectin) ELISA Kit, human 161 scd62p (sp-selectin) ELISA Kit, human 161 scd8 ELISA Kit, human 160 SCF antibody (mab) 109 SCF antibody 109 SCF EDK, human 166 SCF EDK, mouse 166 SCF EDK, rat 166 SCF ELISA Kit, human 159 SCF ELISA Kit, mouse 159 SCF, human, recombinant (E. coli) 178 SCF, human, recombinant (Sf9) 178 SCF, mouse, recombinant 183 SCF, rat, recombinant 185 SCGF-alpha, human, recombinant 178 SCGF-beta antibody 109 SCGF-beta, human, recombinant 178 SCO1 antibody 142 SCO2 antibody 143 SCRAPPER (CT) antibody 134 SCRAPPER (NT) antibody 134 SDF-1 alpha (CXCL12), human, recombinant 178 SDF-1 alpha (CXCL12), mouse, recombinant 183 SDF-1 alpha (CXCL12), rat, recombinant 185 SDF-1 alpha antibody 109 SDF-1 alpha ELISA Kit, human 159 SDF-1 alpha ELISA Kit, mouse 159 SDF-1 beta (CXCL12), human, recombinant 178 SDF-1 beta (CXCL12), mouse, recombinant 183 SDF-1 beta (CXCL12), rat, recombinant 185 SDF-1 beta antibody 109 SDIP/Europium 17 sdll-4, human, recombinant 171 segf-r, human, recombinant 171 Senescence Detection Kit 10 Set of four Magnet Bar Plates 84 sfas Ligand, human, recombinant 171 sfas Receptor (CD95), human, recombinant 171 sfgfr-1, human, recombinant (Sf9) 172

219 Alphabetical Index 217 Description Page Description Page sfgfr-2, human, recombinant (Sf9) 172 sfgfr-3, human, recombinant (Sf9) 172 sfgfr-4, human, recombinant (Sf9) 172 sfrp-1, human, recombinant 172 sgp130 ELISA Kit, human 159 Shh, human, recombinant (InC) 178 SHP2 / PTP-2C (CT) antibody 118 SHP2 / PTP-2C (NT) antibody 118 SIGIRR (CT) antibody 140 sil-2r alpha (CD25), human, recombinant 174 sil-4 Receptor (CD124), human, recombinant 174 sil-6 Receptor (CD126), human, recombinant 174 siprotect RNase Inhibitor 74 SIRPa / SHPS-1 (CT) antibody 118 SIRT2 (CT) antibody 143 SIRT2 (NT) antibody 143 Ski antibody 118 SkiP antibody 118 SLC antibody 110 Slc22A17 antibody 143 Slc35D1 (CT) antibody 143 Slc35D1 (NT) antibody 143 Slitrk1 (CT) antibody 134 Slitrk1 (IN) antibody 134 Slitrk2 (CT) antibody 134 Slitrk2 (IN) antibody 134 Slitrk3 (CT) antibody 134 Slitrk3 (IN) antibody 134 Slitrk4 (CT) antibody 134 Slitrk4 (NT) antibody 134 Slitrk5 (IN1) antibody 134 Slitrk5 (IN2) antibody 135 Slitrk6 (CT) antibody 135 Slitrk6 (IN) antibody 135 SLPI antibody 147 Slug (IN1) antibody 118 Slug (IN2) antibody 118 slyve-1 antibody (mab) 104 slyve-1 antibody 104 slyve-1, human, recombinant (Sf9) 176 slyve-1, mouse, recombinant (Sf9) 182 Smac / DIABLO (CT) antibody 129 SMCC-activated Allophycocyanin, lyophilized 56 SMCC-activated R-Phycoerythrin, lyophilized 56 SnoN antibody 118 SOCS1 / JAB (CT) antibody 118 SOCS1 / JAB (NT) antibody 118 SOD Assay Kit 25 SODD (NT) antibody 130 SPQ 21 srank Ligand antibody 130 srank Receptor antibody 108 srank Receptor, human, recombinant 178 srankl (total) ELISA Kit, human 159 srankl antibody (mab) 109 srankl antibody 109 srankl EDK, human 166 srankl EDK, mouse 166 srankl ELISA Kit, human 159 srankl, human, recombinant 178 srankl, mouse, recombinant 183 srankl, rat, recombinant 185 ssdna (APOSTAIN) antibody (mab) 130 ST2 (NT) antibody 140 StarGate Acceptor Vectors 71 StarGate Entry Cloning Set 71 StarGate Fusion Cloning Set 1 (IRES1) 71 StarGate Fusion Cloning Set 2 (SD1) 71 StarGate Fusion Cloning Set 3 (LINK1) 71 StarGate Mutagenesis Entry Cloning Set 71 StarGate Transfer Reagent Set 71 STAT1 (CT) antibody 118 Staurosporine 44 STEAP1 (CT) antibody 143 STEAP1 (IN) antibody 143 STEAP2 antibody 143 STEAP3 antibody 143 STEAP4 antibody 143 stie-1/fc Chimera, human, recombinant (Sf9) 179 stie-1/fc Chimera, mouse, recombinant (Sf9) 183 stie-2/fc Chimera, human, recombinant (Sf9) 179 stie-2/fc Chimera, mouse, recombinant (Sf9) 183 STIM1 antibody 110 STIM2 (CT) antibody 110 STIM2 (IN) antibody 110 stnf-r (60 kda) antibody (mab) 111 stnf-r1 (CD120a), human, recombinant 179 stnf-r2 (CD120b), human, recombinant 179 stnf-receptor Inhibitor, human, recombinant 179 stnf-ri antibody (mab) 111 stnf-ri antibody 112 stnf-rii antibody (mab) 111 stnf-rii antibody 112 strail (APO2L) EDK, human 166 strail Receptor-1 (DR4), human, recombinant 179 strail Receptor-2 (DR5), human, recombinant 179 Streptavidin-Allophycocyanin 58 Streptavidin-Allophycocyanin (cross-linked) 58 Streptavidin-B-Phycoerythrin 58 Streptavidin-Fluorescein-5-isothiocyanate (FITC) 58 Streptavidin-R-Phycoerythrin 58 Sulforhodamine Sulforhodamine 101 Labeling Kit-NH Sulforhodamine 101-a-bungarotoxin 16 Sulforhodamine B 17 Sulforhodamine G 18 Sumo antibody 118 Superoxide Dismutase (SOD, human) 25

220 218 Alphabetical Index Description Page Description Page Survivin / TIAP (CT) antibody 130 Survivin / TIAP (NT) antibody 130 svegfr-1 (flt-1, D1-3), human, recombinant (Sf9) 180 svegfr-1 (flt-1, D1-4), human, recombinant (Sf9) 180 svegfr-1 (flt-1, D1-5), human, recombinant (Sf9) 180 svegfr-1 (flt-1, D7-Fc), human, recombinant (Sf9) 180 svegfr-1 (flt-1, native), human, recombinant (Sf9) 180 svegfr-2 (skdr, D7), human, recombinant (Sf9) 180 svegfr-2 (skdr-fc, D7-Fc), human, recombinant (Sf9) 180 svegfr-3 (flt-4, native) human, recombinant (Sf9) 180 SynapseGreen C1 16 SynapseGreen C18 (FM3-25) 16 SynapseGreen C2 (FM2-10) 16 SynapseGreen C3 16 SynapseGreen C4 (FM1-43) 16 SynapseGreen C5 (FM1-84) 16 SynapseRed C1 16 SynapseRed C2 (FM4-64) 16 SynapseRed C2M (FM5-95) 16 Synapsin antibody 110 Syntaphilin (IN) antibody 135 Syntaphilin (NT) antibody 135 TAB1 antibody 140 TACE / ADAM17 (CT) antibody 130 TACI antibody 110 TACI antibody 130 TACI EDK, human 166 TACI, human, recombinant 178 TAFA-2, human, recombinant 178 TAK1 / MAP3K7 (NT) antibody 118 Tamoxifen citrate 44 TANK / TBK1 (CT) antibody 119 TANK / TBK1 (NT) antibody 119 TARC (CCL17), human, recombinant 178 TARC antibody 110 TBC1D1 (CT) antibody 143 T-cadherin / Heart-cadherin (NT) antibody 119 TCCR (CT) antibody 130 TCCR (NT) antibody 130 TCR alpha antibody 110 TCR beta antibody 110 TDP43 (IN) antibody 135 TDP43 (NT) antibody 135 TECK (CCL25), human, recombinant 178 TECK antibody (mab) 110 TECK antibody 110 TECK ELISA Kit, mouse 159 TEM1 (CT) antibody 143 TEM1 (NT) antibody 143 TEM2 (CT) antibody 143 TEM2 (NT) antibody 143 T TEM4 (IN) antibody 143 TEM5 (CT) antibody 143 TEM5 (IN) antibody 143 TEM7 (CT) antibody 143 TEM7 (IN) antibody 143 Tetrabromorhodamine 123, bromide 13 Tetramethylrhodamine-a-bungarotoxin 16 Tetrodotoxin, citrate-free 16 Tetrodotoxin, with citrate 16 Texas Red-DHPE 15 TexasRed PCR Labeling Kit 51 TFF-1 antibody 110 TFF-1, human, recombinant 178 TFF-2 antibody 110 TFF-2, human, recombinant 178 TGF-alpha antibody 110 TGF-alpha ELISA Kit, human 159 TGF-alpha, human, recombinant 178 TGF-beta 1 (mature) antibody 110 TGF-beta 1 antibody 110 TGF-beta 1 ELISA Kit, human 159 TGF-beta 1 ELISA Kit, mouse 159 TGF-beta 1 ELISA Kit, rat 159 TGF-beta 1, human, recombinant (A293 cells) 179 TGF-beta 1, human, recombinant (CHO) 178 TGF-beta 2 antibody 110 TGF-beta 2 ELISA Kit, human 159 TGF-beta 2, human, recombinant (A293 cells) 179 TGF-beta 2, human, recombinant (Sf9) 179 TGF-beta 3 antibody 110 TGF-beta 3, human, recombinant 179 TGF-beta 5 antibody 110 TGF-beta antibody (mab) 110 TGF-beta R1 antibody 110 TGF-beta R2 antibody 110 TGF-beta R3 antibody 110 TGFR-1 /ALK-1 antibody (mab) 110 TGFR-1 /ALK-5 antibody (mab) 110 Thapsigargin 44 Thrombin, human, recombinant 179 Thymosin a1 ELISA Kit, human 161 Thymosin b4 ELISA Kit, human 161 Thymulin ELISA Kit, human 161 Thyreoglobulin (htg) ELISA Kit, human 163 Thyreoglobulin (htg, recovery) ELISA Kit, human 163 TIE-1 antibody (mab) 111 TIE-2 antibody (mab) 111 TIE-2 ELISA Kit, human 159 TIGAR (IN1) antibody 130 TIGAR (IN2) antibody 130 TIM-1 / Kim-1 (IN) antibody 111 TIM-1 / Kim-1 (NT) antibody 111 TIM-4 / SMUCKLER (CT) antibody 111 TIM-4 / SMUCKLER (IN) antibody 111

221 Alphabetical Index 219 Description Page Description Page TIMP-1 antibody (mab) 111 TIMP-1 ELISA Kit, human 159 TIMP-1 ELISA Kit, mouse 159 TIMP-1 ELISA Kit, rat 159 TIMP-1, human, recombinant 179 TIMP-2 antibody (mab) 111 TIMP-2 ELISA Kit, human 159 TIMP-2 ELISA Kit, mouse 159 TIMP-3 antibody (mab) 111 TIMP-4 antibody (mab) 111 TIP47 (CT) antibody 119 TIP47 (NT) antibody 119 TIRAP (CT) antibody 140 TIRAP / MAL (IN) antibody 140 TIRP (C2) antibody 140 TIRP (CT) antibody 140 TL-1 antibody 111 TL1A (NT) antibody 130 TL1A antibody 130 TL-1A EDK, human 166 TL-1A, human, recombinant 179 TLR1 (IN) antibody 140 TLR1 (NT) antibody 140 TLR10 (IN) antibody 140 TLR11 (CT) antibody 140 TLR11 (IN) antibody 140 TLR2 (NT) antibody 140 TLR3 (CD283), human, recombinant 179 TLR3 (CT) antibody 140 TLR3 (NT) antibody 140 TLR4 (NT) antibody 140 TLR5 (CT) antibody 140 TLR5 (IN) antibody 140 TLR6 (IN) antibody 140 TLR6 (NT) antibody 140 TLR7 (IN) antibody 140 TLR8 (IN) antibody 140 TLR9 (CT) antibody 140 TLR9 (IN) antibody 140 TMP21 (CT) antibody 135 TMP21 (IN) antibody 135 TMRE 13, 17 TMRM 13, 17 TNF-alpha (total) ELISA Kit, human 159 TNF-alpha antibody (mab) 111 TNF-alpha antibody 111 TNF-alpha EDK, human 166 TNF-alpha EDK, mouse 166 TNF-alpha EDK, rat 166 TNF-alpha ELISA Kit, human 159 TNF-alpha ELISA Kit, monkey 159 TNF-alpha ELISA Kit, mouse 159 TNF-alpha ELISA Kit, rat 159 TNF-alpha M (151 aa), human, recombinant 179 TNF-alpha, human, recombinant (E. coli) 179 TNF-alpha, human, recombinant (Yeast) 179 TNF-alpha, mouse, recombinant (E. coli) 184 TNF-alpha, mouse, recombinant (S. lividans) 184 TNF-alpha, rat, recombinant 185 TNF-beta antibody (mab) 111 TNF-beta antibody (mab), FITC 111 TNF-beta antibody 111 TNF-beta ELISA Kit, human 159 TNF-beta, human, recombinant 179 TNF-beta, human, recombinant (InC) 179 TNF-R (60 kda) ELISA Kit, human 159 TNF-R (80 kda) ELISA Kit, human 159 TNF-R I ELISA Kit, monkey 159 TNF-R II ELISA Kit, human 160 TNF-Receptor Fusion Protein, human, recombinant 179 TNFRSF14 / HVEM (CT) antibody 137 TNFRSF14 / HVEM (NT) antibody 137 TNFRSF14 / HVEM / TR2 (CT) antibody 112 TNFRSF14 / HVEM / TR2 (NT) antibody 112 TNPO3 (NT) antibody 143 TNPO3 (NT2) antibody 143 TOCA-1 (CT) antibody 135 TOCA-1 (NT) antibody 135 TOLLIP (CT) antibody 140 TOLLIP (IN) antibody 141 TOR / FRAP / RAFT antibody 119 Toso / FAIM3 antibody 130 Total RNA Isolation Kit I 27 Total RNA Isolation Kit II 27 TP1 antibody 143 t-pa ELISA Kit, human 160 TPEN 21 TPO (MDGF) EDK, human 166 TPO antibody 112 TPO ELISA Kit, human 160 TPO ELISA Kit, mouse 160 TPO, human, recombinant (CHO) 179 TPO, human, recombinant (E. coli) 179 TPO, mouse, recombinant 184 TRAF2 (CT) antibody 119 TRAF2 (NT) antibody 119 TRAF3 / CD40BP / LAP1 (CT) antibody 119 TRAF3 / CD40BP / LAP1 (NT) antibody 119 TRAF6 (CT) antibody 119 TRAIL (Apo2L), human, recombinant 179 TRAIL (Apo2L), mouse, recombinant 184 TRAIL / Apo-2L (CT) antibody 130 TRAIL / Apo2L antibody (mab) 112 TRAIL / Apo2L antibody 112 TRAIL ELISA Kit, human 160, 162 TRBP1 (IN) antibody 137 TRIAD3A antibody 141 TRIF / TICAM-1 antibody 141

222 220 Alphabetical Index Description Page Description Page TRIM25 (CT) antibody 143 TRIM25 (NT) antibody 143 TRIM5α (CT) antibody 137 TRIM5α (IN1) antibody 137 TRIM5α (IN2) antibody 137 TRIM5γ antibody 137 TRIM5δ antibody 137 TRITC-DHPE 15 TRPC3 (CT) antibody 119 TRPC3 (NT) antibody 119 TRPC6 (CT) antibody 119 TRPC6 (NT) antibody 119 TRT antibody 144 Trypan Blue 14 Trypan Blue Solution (0.4%) 14 TSC1 / Hamartin (CT) antibody 119 TSC1 / Hamartin (IN) antibody 119 TSC2 / Tuberin (CT) antibody 119 TSC2 / Tuberin (NT) antibody 119 TSG, human, recombinant 179 TSLP (CT) antibody 112 TSLP (IN) antibody 112 TSLP antibody 112 TSLP EDK, human 166 TSLP Receptor antibody 112 TSLP, human, recombinant 179 TTC5 antibody 119 TWEAK antibody 112 TWEAK antibody 130 TWEAK EDK, human 166 TWEAK Receptor, human, recombinant 180 TWEAK, human, recombinant 179 Tyrphostin AG Tyrphostin AG UBC13 antibody 141 UEV1A antibody 141 Unc93a antibody 144 Unc93b antibody 147 UNG1 (CT) antibody 139 UNG1 (NT) antibody 139 UNG2 (IN) antibody 139 UNG2 (NT) antibody 139 UROP11 antibody 149 Urotensin II ELISA Kit, human 163 U VAP-1 ELISA Kit, human 160 Vaspin antibody 112 Vaspin, human, recombinant 180 VCAM-1 / CD106 antibody (mab) 112 VCAM-1 / CD106 antibody (mab), biotin 112 VCAM-1 / scd106 ELISA Kit, human 160 VCAM-1 / scd106 ELISA Kit, mouse 160 VDBP ELISA Kit, human 163 VE-Cadherin antibody (mab) 112 VE-cadherin antibody 130 VE-cadherin antibody, biotin 130 VE-cadherin antibody, FITC 130 VE-Cadherin ELISA Kit, human 162 VE-Cadherin ELISA Kit, monkey 162 VEGF EDK, human 166 VEGF EDK, mouse 166 VEGF, rat, recombinant 185 VEGF-121, human, recombinant 180 VEGF-121, human, recombinant (Sf9) 180 VEGF-121, mouse, recombinant 184 VEGF-145, human, recombinant (Sf9) 180 VEGF-164, mouse, recombinant 184 VEGF-165, human, recombinant (CHO) 180 VEGF-165, human, recombinant (E. coli) 180 VEGF-165, human, recombinant (Sf9) 180 VEGF-165, mouse, recombinant (E. coli) 184 VEGF-165, mouse, recombinant (Sf9) 184 VEGF-189, human, recombinant 180 VEGF-A antibody (mab) 112 VEGF-A antibody 112 VEGF-A ELISA Kit, human 160 VEGF-A ELISA Kit, mouse 160 VEGF-A ELISA Kit, rat 160 VEGF-B antibody (mab) 113 VEGF-B antibody 113 VEGF-C antibody (mab) 113 VEGF-C antibody 113 VEGF-C ELISA Kit, human 160 VEGF-C ELISA Kit, rat 160 VEGF-C, human, recombinant 180 VEGF-C, rat, recombinant 185 VEGF-C152S, rat, recombinant 185 VEGF-D antibody (mab) 113 VEGF-D antibody 113 VEGF-E, Orv virus, recombinant 180 V

223 Alphabetical Index 221 Description Page Description Page VEGF-I, human, recombinant 180 VEGF-R I ELISA Kit, human 160 VEGF-R II ELISA Kit, human 160 VEGFR-1 / flt-1 antibody (mab) 113 VEGFR-1 / flt-1 antibody 113 VEGFR-2 / FLK-1 antibody (mab) 113 VEGFR-2 / KDR antibody (mab) 113 VEGFR-2 / KDR antibody 113 VEGFR-3 / flt-4 antibody (mab) 113 VEGFR-3 / flt-4 antibody 113 VGF antibody 135 Vimentin antibody 114 VISA (IN) antibody 147 VISA (NT) antibody 147 Visfatin antibody 114 Visfatin, human, recombinant 180 X, Y, Z XAF-1 (CT) antibody 130 XAF-1 (IN) antibody 130 XBP-1 (CT) antibody 119 XBP-1 (NT) antibody 119 XEDAR / TNFRSF27 antibody 119 X-Gal 12 XIAP (CT) antibody 130 XTT 8 ZBP1 antibody 147 Zinquin ethyl ester 21 Zinquin free acid 21 ZIPK (IN) antibody 130 Zonulin ELISA Kit, human 163 Visfatin, mouse, recombinant 184 VPS53 (CT) antibody 144 VPS53 (IN) antibody 144 W WISP-1, human, recombinant 180 WISP-3, human, recombinant 180 Wnt-1 antibody 114 WNT-1, human, recombinant 180 Wnt-10a antibody 114, 119 Wnt-10b antibody 114, 119 Wnt-2 antibody 114 Wnt-3a antibody 114 Wnt-4 antibody 114 WNV Core (CT) antibody 138 WNV Core (NT) antibody 138 WNV Env (CT) antibody 138 WNV Env (IN) antibody 138 WNV Env (NT) antibody 138 WNV Matrix (CT) antibody 138 WNV Matrix (IN) antibody 138 WST-1 8 WST-3 8 WST-5 8 WST-8 8

224 222 International Alphabetical Index Distributors International Distributors Australia Austria Bangladesh Bosnia-Herzogovina Bulgaria China Croatia Czech Republic Denmark Finland Hungary India Indonesia Israel Italy Japan Korea Liechtenstein Banksia Scientific Company 3/160 Lytton Road PO Box 529 Bulimba QLD 4171 Australia Biomedica Medizinprodukte GmbH & Co KG Divischgasse Wien Austria Krishgen BioSystems 135/37 Zaveri Bazar Mumbai India Biomedica Medizinprodukte GmbH & Co KG Divischgasse Wien Austria Biomedica Medizinprodukte GmbH & Co KG Divischgasse Wien Austria Shanghai Weike Biochemical Reagent Co. Ltd. Room 3205, Universial Mansion No. 172 Yu Yuan Road Shanghai Biomedica Medizinprodukte GmbH & Co KG Divischgasse Wien Austria Biomedica Medizinprodukte GmbH & Co KG Divischgasse Wien Austria Labinova AB Centralvägen Upplands Väsby Sweden Biotop Oy Tykistökatu 6B Turku Finland Biomedica Medizinprodukte GmbH & Co KG Divischgasse Wien Austria Krishgen BioSystems 135/37 Zaveri Bazar Mumbai India Krishgen BioSystems 135/37 Zaveri Bazar Mumbai India Biological Industries Ltd. Kibbutz Beit Haemek, Israel International PBI S.p.A. Via Novara, Milano Italy Funakoshi Co. Ltd. 9-7 Hongo 2-chome Bunkyo-ku Tokyo Japan Sungwoo Life Science Co., Ltd Samsung BLDG 4th Floor Uijeongbu 2Dong Uijeongbu-City Kyeonggi_Do South Korea BioConcept Innovationszentrum Nordwestschweiz Postfach 427 Paradiesrain Allschwil 1 Switzerland Phone: Fax: Free Call: sales@banksiascientific.com.au Internet: Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: sales@krishgen.com Internet: Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: info@weike21.com Internet: Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: info@labinova.se Internet: Phone: Fax: myynti@biotop.fi Internet: Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: sales@krishgen.com Internet: Phone: Fax: sales@krishgen.com Internet: Phone: Fax: info@bioind.com Internet: Phone: Fax: info@internationalpbi.it Internet: Phone: Fax: reagent@funakoshi.co.jp Internet: Phone: Fax: sungwoo@sungwools.com Internet: Phone: Fax: info@bioconcept.ch Internet:

225 International Alphabetical Distributors Index 223 Macedonia Nepal Netherlands New Zealand Norway Poland Portugal Romania Serbia-Montenegro Singapore Slovakia Slovenia Spain Sri Lanka Sweden Switzerland Taiwan Thailand Biomedica Medizinprodukte GmbH & CoKG Divischgasse Wien Austria Krishgen BioSystems 135/37 Zaveri Bazar Mumbai India ITK Diagnostics BV P.O. Box 73 Joh. Enschedeweg AB Uithoorn Netherlands Banksia Scientific Company 12 Brigade Street Airport Oaks Auckland New Zealand Labinova AB Centralvägen Upplands Väsby Sweden Biomedica Medizinprodukte GmbH & Co KG Divischgasse Wien Austria Labclinics, S.A. c/industria Barcelona Spain Biomedica Medizinprodukte GmbH&CoKG Divischgasse Wien Austria Biomedica Medizinprodukte GmbH & Co KG Divischgasse Wien Austria Biomed Diagnostics Pte. Ltd. 18 Boon Lay Way # TradeHub 21 Singapore Biomedica Medizinprodukte GmbH & Co KG Divischgasse Wien Austria Biomedica Medizinprodukte GmbH & Co KG Divischgasse Wien Austria Labclinics, S.A. c/industria Barcelona Spain Krishgen BioSystems 135/37 Zaveri Bazar Mumbai India Mediqip AB Förrådsvägen Huddinge Sweden BioConcept Innovationszentrum Nordwestschweiz Postfach 427 Paradiesrain Allschwil 1 Switzerland Biochiefdom International Co. Ltd. 1F, No 50, Yishou St. Wensham District Taipei 116 Taiwan Biomed Diagnostics (Thailand) Co. Ltd. 96/38 Moo 6 Bangkruai-Sainoi Rd Bangkruai Nontaburi, Thailand Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: sales@krishgen.com Internet: Phone: Fax: info@itk.nl Internet: Phone: Fax: sales@banksiascientific.co.nz Internet: Phone: Fax: info@labinova.se Internet: Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: info@labclinics.com Internet: Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: sales@biomed.com.sg Internet: Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: sales.biomedica@bmgrp.at Internet: Phone: Fax: info@labclinics.com Internet: Phone: Fax: sales@krishgen.com Internet: Phone: Fax: info@mediqip.se Internet: Phone: Fax: info@bioconcept.ch Internet: Phone: Fax: info@bio-chief.com.tw Internet: Phone: Fax: nan@biomedthai.com Internet:

226 224 Notes Notes

227 Notes Notes 225