Phyllom BioProducts. Protecting the Beauty, Health and Value of Forests, Farms and Landscapes. grubgone! & beetlegone!

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1 Phyllom BioProducts Protecting Forests, Farms and Landscapes grubgone! & beetlegone! Protecting the Beauty, Health and Value of Forests, Farms and Landscapes 10/9/2015 1

2 Novel & Natural Bacillus thuringiensis galleriae strain SDS-502 Novel beetle active Cry8Da protein Soil and Foliar active BT formulas EPA reg EPA reg No adverse risk for all non targets tested for EPA registration 40 CFR Exemption from requirement for a tolerance for edibles Phyllom BioProducts technologies are protected by issued patents 2

3 Family Common Name Latin Name Test Stage Hosts Scarabaeidae Asiatic Garden Beetle Maladera Castenea Larva Grasses, Food crops Japanese beetle Popillia japonica Adult/Larva Grasses, Food crops, Nursery May/June beetle Phyllophaga spp. Larva Grasses, Ornamentals Green June beetle Cotinis nitida Larva Grasses, Food crops Oriental beetle Anomala orientalis Adult/Larva Grasses, Food crops European Chafer Rhizotrogus majalis Larva Grasses Cupreous Chafer Anomala cuprea Larva Grasses N. Masked Chafer Cyclocephala borealis Larva Grasses S. Masked Chafer Cyclocephala lurida Larva Grasses Cuculionidae Annual bluegrass weevil Listronotus maculicolis Larva Annual bluegrass (Poa) Buprestidae Emerald Ash Borer Agrilus planipennis Adult Ash Gold Spotted Oak Borer Agrilus spp. Adult Oak Chyrsomelidae Alder leaf beetle Agelastica alni Adult Alder Tenebrionidae Darkling beetle Alphitobius diaperinus Adult/Larva Poultry 10/9/2015 3

4 100 b b b b b b controls followed by same letter not significantly LSD % control Shetlar/Andon OH St. U. grubgone 99 DAT 2.5 lb/k grubex 99 DAT 3 lb/k grubgone 86 DAT 2.5 lb/k Meridian 86 DAT 1.83 lb/k grubgone 60 DAT 2.5 lb/k Meridian 60 DAT 1.83 lb/k 10/9/ a Check grubgone! is a registered trademark of Phyllom BioProducts Corp. grubex is a registered trademark of Scotts Co. Meridian is a regstered trademark of Syngenta.

5 100% 90% a a a 80% 70% 60% 50% 40% 30% 20% 10% 0% Royals/Brandenburg,NC St. U. grubgone! G 2.0 lb/1000 sq ft grubgone! G 3.0 lb/1000 sq ft Merit 0.5G 1.83 lb/1000 sq ft controls followed by same letter not significantly LSD 0.05 grubgone! is a registered trademark of Phyllom BioProducts Corp. Merit is a registered trademark of Bayer Crop Science. 10/9/2015 5

6 100% 90% 80% ab a ab ab controls followed by same letter not significantly LSD % b 60% 50% 40% 30% 20% 10% Royals/Brandenburg,NC St. U. 0% grubgone! G 2 lb/1000 sq ft grubgone! G 3 lb/1000 sq ft Dylox 9.3 G 2 lb/1000 sq ft Bayer Advanced G 3 lb/1000 sq ft Scott s grubex 1 G 3 lb 1000 sq ft grubgone! is a registered trademark of Phyllom BioProducts Corp. grubex is a registered trademark of Scotts Co. Dylox and Bayer Advanced are regstered trademark s of Bayer. 10/9/2015 6

7 2009 Control of masked chafer & Japanese beetle grubs with grubgone! G curative treatment assessed 38 DAT 100.0% 90.0% 80.0% 70.0% % Control 60.0% 50.0% 40.0% 30.0% 20.0% 10.0% 0.0% Untreated Andon/Shetlar Ohio St. U. grubgone! G 115lb/A Treatment Merit.5G 60lb/A 7

8 2010 Control of So. Masked Chafer 3rd instar turf grubs with grubgone! G curative 35 DAT 70.0% 60.0% 50.0% 3 rd instar grubs % Control 40.0% 30.0% 20.0% 10.0% 0.0% Untreated Stamm/Baxendale Univ NE grubgone! G 153lb/A Treatment Dylox 6.2G 130lb/A 10/9/2015 8

9 cd d controls followed by same letter not significantly LSD Applied July 13 & July grubgone! G 2 lb/1000 sq ft clothianidin +bifenthrin Aloft GC 0.33 oz/1000 sq ft a Check grubgone! is a registered trademark of Phyllom BioProducts Corp., Aloft and Aloft GC S C logos are registered trademarks of Arysta Lifesciences North America, LLC 10/9/2015 9

10 2009 grubgone! G curative control of Oriental beetle grubs Assessed 36 DAT Hickory Ridge GC, Amherst, MA 100.0% 90.0% 80.0% 70.0% b b % Control 60.0% 50.0% 40.0% 30.0% 20.0% 10.0% 0.0% Vittum, U. Mass. a Untreated grubgone! G 115lb/A Merit.5G 60lb/A Treatment Numbers followed by the same letter are not significantly different, Fisher s Protected LSD; F = 5.10, P < , significance level = /9/

11 Turf & Ornamental use sites with no geographical restrictions Controls scarab & weevil grubs with 1 application/generation Preventative or Curative against 1 st -3 rd instar grubs No adverse risk to all non targets tested REI when dust settles 10/9/

12 Untreated beetlegone! (2 oz/gl) beetlegone! (4 oz/gl) Permethrin 8 floz/100 gl Hr 1 DAT 3 DAT 5 DAT 7 DAT 10/9/

13 Untreated beetlegone! (2 oz/gl) beetlegone! (4 oz/gl) Permethrin 8 floz/100 gl HR 1 DAT 3 DAT 5 DAT 7 DAT 10/9/

14 100 3rd instar Japanese beetle grub % control after fall nursery plant insecticide dips & 150 DAT assessment McMinnville, TN Talstar NF.0125 lb.a.i./100 gl Onyx Pro.0125 lb.a.i./100 gl Sevin.125 lb.a.i./100 gl Amorex 1 ml/gl Dylox 420SL.125 lb.a.i./100 gl beetlegone! tlc 1 oz./gl beetlegone! tlc 2 oz./gl 0 Balled & Burlap dipped # 3 container pine bark mix dipped Contributors: Jason Oliver1, Karla Addesso1, Christopher Ranger2, Michael Reding2, James Moyseenko2, Nadeer Youssef2 and Paul O Neal 1 1Tennessee State University, College of Agriculture, Human, & Natural Sciences, Otis L. Floyd Nursery Research Center, 2 USDA-Agricultural Research Service, Horticultural Insects Research Lab, Wooster, OH, 10/9/

15 100 3rd instar Japanese beetle grub % control after fall nursery plant insecticide drench & 150 DAT assessment McMinnville, TN Lorsban.125 lb.a.i./100 gl Sevin 1.0 lb.a.i./100 gl Sevin.75 lb.a.i./100 gl Dylox 420SL.5 lb.a.i./100 gl Dylox 420SL.25 lb.a.i./100 gl Onyx Pro.125 lb.a.i./100 gl Onyx Pro.0625 lb.a.i./100 gl beetlegone! tlc 1 oz./gl beetlegone! tlc 2 oz./gl 10 0 # 15 pot pine bark mix topical drench 10/9/

16 10/9/

17 Trees/Shrubs/Annuals/Edibles Active against Scarab & Buprestid adults No adverse risk to any non targets tested REI when sprays dry For Organic Production 10/9/

18 Peak Adult Flight 1000 degree days Adults feed & mate on ash leaves degree days Adults emerge from under bark Females lay eggs on or under bark Pupation Larvae develop (1-4 instars) feed on phloem 18

19 10/9/

20 Economical for Ash Dense Sites No Wounds & Tree Size Does Not Matter! Organic treatment option for sensitive areas Low impact to non target species Bt is a time tested tool for Forestry IPM 20

21 10/9/

22 Phyllom BioProducts Target the Pest, Not the Rest! grubgone! beetlegone! boregone! 10/9/

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