Kit components. GoTaq DNA Polymerase, 100u. GoTaq DNA Polymerase 5X Green GoTaq Reaction Buffer 5X Colorless GoTaq Reaction Buffer

Transcription

1 06/09/2016 Kit components Product code M3171 Components: M830 M791 M792 Description GoTaq DNA Polymerase, 100u GoTaq DNA Polymerase 5X Green GoTaq Reaction Buffer 5X Colorless GoTaq Reaction Buffer

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3 Page 1/7 * SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier Trade name: GoTaq DNA Polymerase Article number: M Relevant identified uses of the substance or mixture and uses advised against No further relevant information available. Application of the substance / the preparation: Laboratory chemicals 1.3 Details of the supplier of the safety data sheet Manufacturer/Supplier: Promega Corporation 2800 Woods Hollow Road Madison, WI U.S.A SDS author: Regulatory.Affairs@promega.com Further information obtainable from: Promega UK Delta House Southampton Science Park Southampton SO16 7NS United Kingdom Tel: (44) Fax: (44) Freephone: Address: ukcustserve@promega.com 1.4 Emergency telephone number: For Chemical Emergency Spill, Leak, Fire, Exposure, or Accident Call CHEMTREC Day or Night Within USA and Canada: Outside USA and Canada: (collect calls accepted). Within UK +(44) * SECTION 2: Hazards identification 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 Aquatic Chronic 3 H412 Harmful to aquatic life with long lasting effects. 2.2 Label elements Labelling according to Regulation (EC) No 1272/2008 The product is classified and labelled according to the CLP regulation. Hazard pictograms Signal word Hazard statements H412 Harmful to aquatic life with long lasting effects. (Contd. on page 2)

4 Page 2/7 Trade name: GoTaq DNA Polymerase Precautionary statements P273 Avoid release to the environment. P501 Dispose of contents/container in accordance with local/regional/national/international regulations. 2.3 Other hazards Results of PBT and vpvb assessment PBT: Not applicable. vpvb: Not applicable. (Contd. of page 1) * SECTION 3: Composition/information on ingredients 3.2 Chemical characterisation: Mixtures Description: The product is a mixture of the hazardous substances listed below along with unlisted nonhazardous substances. Components: CAS: EINECS: glycerol substance with a Community workplace exposure limit CAS: Ethoxylated Alkyl Amines Aquatic Chronic 2, H411; Skin Irrit. 2, H315; Eye Irrit. 2, H319; STOT SE 3, H335 Additional information: For the wording of the listed hazard phrases refer to section % 5-10% * SECTION 4: First aid measures 4.1 Description of first aid measures After inhalation: If the patient feels unwell or is concerned, obtain medical advice. After skin contact: Generally the product does not irritate the skin. After eye contact: Rinse opened eye for several minutes under running water. After swallowing: If the patient feels unwell or is concerned, obtain medical advice. 4.2 Most important symptoms and effects, both acute and delayed None 4.3 Indication of any immediate medical attention and special treatment needed No further relevant information available. * SECTION 5: Firefighting measures 5.1 Extinguishing media Suitable extinguishing agents: CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam. 5.2 Special hazards arising from the substance or mixture None known 5.3 Advice for firefighters No special advice. * SECTION 6: Accidental release measures 6.1 Personal precautions, protective equipment and emergency procedures Remove persons from danger area. Wear protective clothing. 6.2 Environmental precautions: Dilute with plenty of water. Do not allow to enter sewers/ surface or ground water. (Contd. on page 3)

5 Page 3/7 Trade name: GoTaq DNA Polymerase 6.3 Methods and material for containment and cleaning up: Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust). 6.4 Reference to other sections No dangerous substances are released. See Section 7 for information on safe handling See Section 13 for disposal information. (Contd. of page 2) * SECTION 7: Handling and storage 7.1 Precautions for safe handling No special precautions are necessary if used correctly. Information about fire - and explosion protection: No special measures required. 7.2 Conditions for safe storage, including any incompatibilities Storage Requirements to be met by storerooms and receptacles: No special requirements. Information about storage in one common storage facility: Not required. Further information about storage conditions: None. 7.3 Specific end use(s) No further relevant information available. * SECTION 8: Exposure controls/personal protection Additional information about design of technical facilities: No further data; see item Control parameters Ingredients with limit values that require monitoring at the workplace: glycerol WEL Long-term value: 10 mg/m³ Additional information: The lists valid during the making were used as basis. 8.2 Exposure controls Personal protective equipment General protective and hygienic measures Wash hands before breaks and at the end of work. Respiratory protection: Not required. Protection of hands: Protective gloves. Select the glove material considering penetration time, rate of diffusion and degradation time. The selected protective gloves must satisfy the specifications of EU Directive 89/686/EEC and the standard EN 374 derived from it or AS/NZS Material of gloves: The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance of the glove material can not be calculated in advance and has therefore to be checked prior to the application. Eye protection: Use equipment for eye protection tested and approved under government standard EN 166(EU) or AS/NZS 1336, 1137 and (Contd. on page 4)

6 Page 4/7 Trade name: GoTaq DNA Polymerase (Contd. of page 3) * SECTION 9: Physical and chemical properties 9.1 Information on basic physical and chemical properties General Information Appearance: Form: Fluid Colour: Colourless Odour: Not determined Odour threshold: ph-value at 20 C: 8 Change in condition Melting point/melting range: undetermined Boiling point/boiling range: 100 C Flash point: > 100 C Flammability (solid, gaseous) Not applicable. Ignition temperature: 400 C Decomposition temperature: Self-igniting: Product is not selfigniting. Danger of explosion: Product does not present an explosion hazard. Explosion limits: Lower: 0.9 Vol % Upper: Vapour pressure at 20 C: 0.1 hpa Density: Not determined Relative density Vapour density Evaporation rate Solubility in / Miscibility with Water: Fully miscible Partition coefficient (n-octanol/water): Viscosity: dynamic: kinematic: Organic solvents: 49.8 % Water: 43.7 % Solids content: 0.1 % 9.2 Other information No further relevant information available. * SECTION 10: Stability and reactivity 10.1 Reactivity No further relevant information available Chemical stability Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications Possibility of hazardous reactions No dangerous reactions known 10.4 Conditions to avoid No further relevant information available Incompatible materials: No further relevant information available. (Contd. on page 5)

7 Page 5/7 Trade name: GoTaq DNA Polymerase 10.6 Hazardous decomposition products: No dangerous decomposition products known (Contd. of page 4) * SECTION 11: Toxicological information 11.1 Information on toxicological effects Acute toxicity Based on available data, the classification criteria are not met. LD/LC50 values relevant for classification: No data available Primary irritant effect: Skin corrosion/irritation Based on available data, the classification criteria are not met. Serious eye damage/irritation Based on available data, the classification criteria are not met. Respiratory or skin sensitisation Based on available data, the classification criteria are not met. CMR effects (carcinogenity, mutagenicity and toxicity for reproduction) Germ cell mutagenicity Based on available data, the classification criteria are not met. Carcinogenicity Based on available data, the classification criteria are not met. Reproductive toxicity Based on available data, the classification criteria are not met. STOT-single exposure Based on available data, the classification criteria are not met. STOT-repeated exposure Based on available data, the classification criteria are not met. Aspiration hazard Based on available data, the classification criteria are not met. * SECTION 12: Ecological information 12.1 Toxicity Aquatic toxicity: Not harmful to the aquatic environment 12.2 Persistence and degradability: Not available 12.3 Bioaccumulative potential Not known 12.4 Mobility in soil No further relevant information available. Ecotoxical effects: Remark: Not available Additional ecological information: General notes: Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water. Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system Results of PBT and vpvb assessment PBT: Not applicable. vpvb: Not applicable Other adverse effects No further relevant information available. * SECTION 13: Disposal considerations 13.1 Waste treatment methods Recommendation Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: Handling and Storage and Section 8: Exposure Control/Personal Protection for additional handling information and protection of employees. Uncleaned packaging: Recommendation: Disposal must be made according to official regulations. Recommended cleansing agents: Water, if necessary together with cleansing agents. (Contd. on page 6)

8 Page 6/7 Trade name: GoTaq DNA Polymerase (Contd. of page 5) * SECTION 14: Transport information 14.1 UN-Number Not hazardous for transportation ADR, ADN, IMDG, IATA 14.2 UN proper shipping name None ADR, ADN, IMDG, IATA 14.3 Transport hazard class(es) None ADR, IMDG, IATA Class ADN ADN/R Class: 14.4 Packing group None ADR, IMDG, IATA 14.5 Environmental hazards: Marine pollutant: Not regulated 14.6 Special precautions for user Not applicable Transport in bulk according to Annex II of Marpol and the IBC Code UN "Model Regulation": No Not applicable. * SECTION 15: Regulatory information 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture Directive 2012/18/EU Named dangerous substances - ANNEX I None of the ingredients is listed. National regulations Classification according to VbF: Class Share in % Wasser 43.7 NK 49.8 Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water Chemical safety assessment: A Chemical Safety Assessment has not been carried out. SECTION 16: Other information This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship. Relevant phrases H315 Causes skin irritation. H319 Causes serious eye irritation. H335 May cause respiratory irritation. H411 Toxic to aquatic life with long lasting effects. (Contd. on page 7)

9 Page 7/7 Trade name: GoTaq DNA Polymerase (Contd. of page 6) Department issuing MSDS: Promega Corporation Safety Department 2800 Woods Hollow Road Madison, WI U.S.A. Contact: Promega UK Delta House, Enterprise Road Chilworth Science Park Southampton SO16 7NS UNITED KINGDOM Free Phone Free Fax Abbreviations and acronyms: ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage of Dangerous Goods by Road) IMDG: International Maritime Code for Dangerous Goods IATA: International Air Transport Association GHS: Globally Harmonised System of Classification and Labelling of Chemicals EINECS: European Inventory of Existing Commercial Chemical Substances ELINCS: European List of Notified Chemical Substances CAS: Chemical Abstracts Service (division of the American Chemical Society) VbF: Verordnung über brennbare Flüssigkeiten, Österreich (Ordinance on the storage of combustible liquids, Austria) LC50: Lethal concentration, 50 percent LD50: Lethal dose, 50 percent PBT: Persistent, Bioaccumulative and Toxic vpvb: very Persistent and very Bioaccumulative Skin Irrit. 2: Skin corrosion/irritation, Hazard Category 2 Eye Irrit. 2: Serious eye damage/eye irritation, Hazard Category 2 STOT SE 3: Specific target organ toxicity - Single exposure, Hazard Category 3 Aquatic Chronic 2: Hazardous to the aquatic environment - Chronic Hazard, Category 2 Aquatic Chronic 3: Hazardous to the aquatic environment - Chronic Hazard, Category 3 * Data compared to the previous version altered.

10 Page 1/7 * SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier Trade name: 5X Green GoTaq Reaction Buffer Article number: M Relevant identified uses of the substance or mixture and uses advised against No further relevant information available. Application of the substance / the preparation: Laboratory chemicals 1.3 Details of the supplier of the safety data sheet Manufacturer/Supplier: Promega Corporation 2800 Woods Hollow Road Madison, WI U.S.A SDS author: Regulatory.Affairs@promega.com Further information obtainable from: Promega UK Delta House Southampton Science Park Southampton SO16 7NS United Kingdom Tel: (44) Fax: (44) Freephone: Address: ukcustserve@promega.com 1.4 Emergency telephone number: For Chemical Emergency Spill, Leak, Fire, Exposure, or Accident Call CHEMTREC Day or Night Within USA and Canada: Outside USA and Canada: (collect calls accepted). Within UK +(44) * SECTION 2: Hazards identification 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 The product is not classified according to the Globally Harmonised System (GHS). 2.2 Label elements Labelling according to Regulation (EC) No 1272/2008 Hazard pictograms Signal word Hazard statements Additional information: Contains Tartrazine. May produce an allergic reaction. available on request. (Contd. on page 2)

11 Page 2/7 Trade name: 5X Green GoTaq Reaction Buffer 2.3 Other hazards Results of PBT and vpvb assessment PBT: Not applicable. vpvb: Not applicable. (Contd. of page 1) * SECTION 3: Composition/information on ingredients 3.2 Chemical characterisation: Mixtures Description: The product is a mixture of the hazardous substances listed below along with unlisted nonhazardous substances. Components: CAS: sucrose, pure substance with a Community workplace exposure limit 25-50% EINECS: Additional information: For the wording of the listed hazard phrases refer to section 16. * SECTION 4: First aid measures 4.1 Description of first aid measures General information: No special measures required. After inhalation: Supply fresh air and to be sure call for a doctor. In case of unconsciousness place patient stably in side position for transportation. After skin contact: Generally the product does not irritate the skin. After eye contact: Rinse opened eye for several minutes under running water. After swallowing: If the patient feels unwell or is concerned, obtain medical advice. 4.2 Most important symptoms and effects, both acute and delayed Allergic reactions 4.3 Indication of any immediate medical attention and special treatment needed No further relevant information available. * SECTION 5: Firefighting measures 5.1 Extinguishing media Suitable extinguishing agents: CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam. 5.2 Special hazards arising from the substance or mixture None known 5.3 Advice for firefighters No special advice. No special advice. Protective equipment: No special measures required. * SECTION 6: Accidental release measures 6.1 Personal precautions, protective equipment and emergency procedures Not required. 6.2 Environmental precautions: Dilute with plenty of water. Do not allow to enter sewers/ surface or ground water. 6.3 Methods and material for containment and cleaning up: Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust). Dispose contaminated material as waste according to Section 13. (Contd. on page 3)

12 Page 3/7 Trade name: 5X Green GoTaq Reaction Buffer 6.4 Reference to other sections No dangerous substances are released. See Section 7 for information on safe handling See Section 13 for disposal information. (Contd. of page 2) * SECTION 7: Handling and storage 7.1 Precautions for safe handling No special measures required. Keep receptacles tightly sealed. Ensure good ventilation/exhaustion at the workplace. Work only in fume cupboard. Information about fire - and explosion protection: Keep respiratory protective device available. The product is not flammable 7.2 Conditions for safe storage, including any incompatibilities Storage Requirements to be met by storerooms and receptacles: No special requirements. Information about storage in one common storage facility: Not required. Further information about storage conditions: None. 7.3 Specific end use(s) No further relevant information available. * SECTION 8: Exposure controls/personal protection Additional information about design of technical facilities: No further data; see item Control parameters Ingredients with limit values that require monitoring at the workplace: sucrose, pure WEL Short-term value: 20 mg/m³ Long-term value: 10 mg/m³ Additional information: The lists valid during the making were used as basis. 8.2 Exposure controls Personal protective equipment General protective and hygienic measures Keep away from foodstuffs, beverages and feed. Immediately remove all soiled and contaminated clothing Do not eat or drink while working. Respiratory protection: In case of brief exposure or low pollution use respiratory filter device. In case of intensive or longer exposure use self-contained respiratory protective device. Protection of hands: Not required. Material of gloves: The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance of the glove material can not be calculated in advance and has therefore to be checked prior to the application. Eye protection: Safety glasses (Contd. on page 4)

13 Page 4/7 Trade name: 5X Green GoTaq Reaction Buffer (Contd. of page 3) * SECTION 9: Physical and chemical properties 9.1 Information on basic physical and chemical properties General Information Appearance: Form: Fluid Colour: Colourless Odour: Not determined Odour threshold: ph-value at 20 C: 8.5 Change in condition Melting point/melting range: Boiling point/boiling range: Flash point: Flammability (solid, gaseous) Ignition temperature: Decomposition temperature: Self-igniting: Danger of explosion: Explosion limits: Lower: Upper: Vapour pressure: undetermined undetermined Not applicable Not applicable. Product is not selfigniting. Product does not present an explosion hazard. Density: Not determined Relative density Vapour density Evaporation rate Solubility in / Miscibility with Water: Fully miscible Partition coefficient (n-octanol/water): Viscosity: dynamic: kinematic: Organic solvents: 0.0 % Water: 43.4 % Solids content: 6.5 % 9.2 Other information No further relevant information available. * SECTION 10: Stability and reactivity 10.1 Reactivity No further relevant information available Chemical stability Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications Possibility of hazardous reactions No dangerous reactions known 10.4 Conditions to avoid No further relevant information available Incompatible materials: No further relevant information available. (Contd. on page 5)

14 Page 5/7 Trade name: 5X Green GoTaq Reaction Buffer 10.6 Hazardous decomposition products: No dangerous decomposition products known (Contd. of page 4) * SECTION 11: Toxicological information 11.1 Information on toxicological effects Acute toxicity Based on available data, the classification criteria are not met. LD/LC50 values relevant for classification: No data available Primary irritant effect: Skin corrosion/irritation Based on available data, the classification criteria are not met. Serious eye damage/irritation Based on available data, the classification criteria are not met. Respiratory or skin sensitisation Based on available data, the classification criteria are not met. CMR effects (carcinogenity, mutagenicity and toxicity for reproduction) Germ cell mutagenicity Based on available data, the classification criteria are not met. Carcinogenicity Based on available data, the classification criteria are not met. Reproductive toxicity Based on available data, the classification criteria are not met. STOT-single exposure Based on available data, the classification criteria are not met. STOT-repeated exposure Based on available data, the classification criteria are not met. Aspiration hazard Based on available data, the classification criteria are not met. * SECTION 12: Ecological information 12.1 Toxicity Aquatic toxicity: Not harmful to the aquatic environment 12.2 Persistence and degradability: Not available 12.3 Bioaccumulative potential Not known 12.4 Mobility in soil No further relevant information available. Ecotoxical effects: Remark: Not available Additional ecological information: General notes: Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water. Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system Results of PBT and vpvb assessment PBT: Not applicable. vpvb: Not applicable Other adverse effects No further relevant information available. * SECTION 13: Disposal considerations 13.1 Waste treatment methods Recommendation Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: Handling and Storage and Section 8: Exposure Control/Personal Protection for additional handling information and protection of employees. Uncleaned packaging: Recommendation: Disposal must be made according to official regulations. Recommended cleansing agents: Water, if necessary together with cleansing agents. (Contd. on page 6)

15 Page 6/7 Trade name: 5X Green GoTaq Reaction Buffer (Contd. of page 5) * SECTION 14: Transport information 14.1 UN-Number Not hazardous for transportation ADR, ADN, IMDG, IATA 14.2 UN proper shipping name None ADR, ADN, IMDG, IATA 14.3 Transport hazard class(es) None ADR, IMDG, IATA Class ADN ADN/R Class: 14.4 Packing group None ADR, IMDG, IATA 14.5 Environmental hazards: Marine pollutant: Not regulated 14.6 Special precautions for user Not applicable Transport in bulk according to Annex II of Marpol and the IBC Code UN "Model Regulation": No Not applicable. * SECTION 15: Regulatory information 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture Directive 2012/18/EU Named dangerous substances - ANNEX I None of the ingredients is listed. National regulations Classification according to VbF: Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water Chemical safety assessment: A Chemical Safety Assessment has not been carried out. SECTION 16: Other information This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship. Department issuing MSDS: Promega Corporation Safety Department 2800 Woods Hollow Road Madison, WI U.S.A. Contact: Promega UK Delta House, Enterprise Road Chilworth Science Park Southampton SO16 7NS (Contd. on page 7)

16 Page 7/7 Trade name: 5X Green GoTaq Reaction Buffer (Contd. of page 6) UNITED KINGDOM Free Phone Free Fax Abbreviations and acronyms: RID: Règlement international concernant le transport des marchandises dangereuses par chemin de fer (Regulations Concerning the International Transport of Dangerous Goods by Rail) ICAO: International Civil Aviation Organisation ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage of Dangerous Goods by Road) IMDG: International Maritime Code for Dangerous Goods IATA: International Air Transport Association GHS: Globally Harmonised System of Classification and Labelling of Chemicals EINECS: European Inventory of Existing Commercial Chemical Substances ELINCS: European List of Notified Chemical Substances CAS: Chemical Abstracts Service (division of the American Chemical Society) VbF: Verordnung über brennbare Flüssigkeiten, Österreich (Ordinance on the storage of combustible liquids, Austria) LC50: Lethal concentration, 50 percent LD50: Lethal dose, 50 percent PBT: Persistent, Bioaccumulative and Toxic vpvb: very Persistent and very Bioaccumulative * Data compared to the previous version altered.

17 Page 1/7 * SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier Trade name: 5X Colorless GoTaq Reaction Buffer Article number: M Relevant identified uses of the substance or mixture and uses advised against No further relevant information available. Application of the substance / the preparation: Laboratory chemicals 1.3 Details of the supplier of the safety data sheet Manufacturer/Supplier: Promega Corporation 2800 Woods Hollow Road Madison, WI U.S.A SDS author: Regulatory.Affairs@promega.com Further information obtainable from: Promega UK Delta House Southampton Science Park Southampton SO16 7NS United Kingdom Tel: (44) Fax: (44) Freephone: Address: ukcustserve@promega.com 1.4 Emergency telephone number: For Chemical Emergency Spill, Leak, Fire, Exposure, or Accident Call CHEMTREC Day or Night Within USA and Canada: Outside USA and Canada: (collect calls accepted). Within UK +(44) * SECTION 2: Hazards identification 2.1 Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 The product is not classified according to the Globally Harmonised System (GHS). 2.2 Label elements Labelling according to Regulation (EC) No 1272/2008 Hazard pictograms Signal word Hazard statements 2.3 Other hazards Results of PBT and vpvb assessment PBT: Not applicable. (Contd. on page 2)

18 Page 2/7 Trade name: 5X Colorless GoTaq Reaction Buffer vpvb: Not applicable. (Contd. of page 1) * SECTION 3: Composition/information on ingredients 3.2 Chemical characterisation: Mixtures Description: The product is a mixture of the hazardous substances listed below along with unlisted nonhazardous substances. Components: CAS: sucrose, pure substance with a Community workplace exposure limit 25-50% EINECS: Additional information: For the wording of the listed hazard phrases refer to section 16. * SECTION 4: First aid measures 4.1 Description of first aid measures General information: No special measures required. After inhalation: If the patient feels unwell or is concerned, obtain medical advice. After skin contact: Generally the product does not irritate the skin. After eye contact: Rinse opened eye for several minutes under running water. After swallowing: If the patient feels unwell or is concerned, obtain medical advice. 4.2 Most important symptoms and effects, both acute and delayed None 4.3 Indication of any immediate medical attention and special treatment needed No further relevant information available. * SECTION 5: Firefighting measures 5.1 Extinguishing media Suitable extinguishing agents: CO2, powder or water spray. Fight larger fires with water spray or alcohol resistant foam. 5.2 Special hazards arising from the substance or mixture None known 5.3 Advice for firefighters No special advice. No special advice. Protective equipment: No special measures required. * SECTION 6: Accidental release measures 6.1 Personal precautions, protective equipment and emergency procedures Not required. 6.2 Environmental precautions: Dilute with plenty of water. Do not allow to enter sewers/ surface or ground water. 6.3 Methods and material for containment and cleaning up: Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust). 6.4 Reference to other sections No dangerous substances are released. See Section 7 for information on safe handling See Section 13 for disposal information. (Contd. on page 3)

19 Page 3/7 Trade name: 5X Colorless GoTaq Reaction Buffer (Contd. of page 2) * SECTION 7: Handling and storage 7.1 Precautions for safe handling No special measures required. Information about fire - and explosion protection: The product is not flammable 7.2 Conditions for safe storage, including any incompatibilities Storage Requirements to be met by storerooms and receptacles: No special requirements. Information about storage in one common storage facility: Not required. Further information about storage conditions: None. 7.3 Specific end use(s) No further relevant information available. * SECTION 8: Exposure controls/personal protection Additional information about design of technical facilities: No further data; see item Control parameters Ingredients with limit values that require monitoring at the workplace: sucrose, pure WEL Short-term value: 20 mg/m³ Long-term value: 10 mg/m³ Additional information: The lists valid during the making were used as basis. 8.2 Exposure controls Personal protective equipment General protective and hygienic measures The usual precautionary measures are to be adhered to when handling chemicals. Respiratory protection: Not required. Protection of hands: Not required. Material of gloves: The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance of the glove material can not be calculated in advance and has therefore to be checked prior to the application. Eye protection: Not required. * SECTION 9: Physical and chemical properties 9.1 Information on basic physical and chemical properties General Information Appearance: Form: Fluid Colour: Colourless Odour: Not determined Odour threshold: ph-value at 20 C: 8.5 Change in condition Melting point/melting range: undetermined Boiling point/boiling range: 100 C Flash point: Not applicable (Contd. on page 4)

20 Page 4/7 Trade name: 5X Colorless GoTaq Reaction Buffer Flammability (solid, gaseous) Ignition temperature: Decomposition temperature: Self-igniting: Danger of explosion: Explosion limits: Lower: Upper: Vapour pressure: Not applicable. Product is not selfigniting. Product does not present an explosion hazard. Density: Not determined Relative density Vapour density Evaporation rate Solubility in / Miscibility with Water: Fully miscible Partition coefficient (n-octanol/water): Viscosity: dynamic: kinematic: Organic solvents: 0.0 % Water: 43.5 % Solids content: 6.5 % 9.2 Other information No further relevant information available. (Contd. of page 3) * SECTION 10: Stability and reactivity 10.1 Reactivity No further relevant information available Chemical stability Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications Possibility of hazardous reactions No dangerous reactions known 10.4 Conditions to avoid No further relevant information available Incompatible materials: No further relevant information available Hazardous decomposition products: No dangerous decomposition products known SECTION 11: Toxicological information 11.1 Information on toxicological effects Acute toxicity Based on available data, the classification criteria are not met. LD/LC50 values relevant for classification: No data available Primary irritant effect: Skin corrosion/irritation Based on available data, the classification criteria are not met. Serious eye damage/irritation Based on available data, the classification criteria are not met. Respiratory or skin sensitisation Based on available data, the classification criteria are not met. CMR effects (carcinogenity, mutagenicity and toxicity for reproduction) Germ cell mutagenicity Based on available data, the classification criteria are not met. Carcinogenicity Based on available data, the classification criteria are not met. Reproductive toxicity Based on available data, the classification criteria are not met. STOT-single exposure Based on available data, the classification criteria are not met. (Contd. on page 5)

21 Page 5/7 Trade name: 5X Colorless GoTaq Reaction Buffer STOT-repeated exposure Based on available data, the classification criteria are not met. Aspiration hazard Based on available data, the classification criteria are not met. (Contd. of page 4) * SECTION 12: Ecological information 12.1 Toxicity Aquatic toxicity: Not harmful to the aquatic environment 12.2 Persistence and degradability: Not available 12.3 Bioaccumulative potential Not known 12.4 Mobility in soil No further relevant information available. Ecotoxical effects: Remark: Not available Additional ecological information: General notes: Water hazard class 1 (German Regulation) (Self-assessment): slightly hazardous for water. Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system Results of PBT and vpvb assessment PBT: Not applicable. vpvb: Not applicable Other adverse effects No further relevant information available. * SECTION 13: Disposal considerations 13.1 Waste treatment methods Recommendation Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: Handling and Storage and Section 8: Exposure Control/Personal Protection for additional handling information and protection of employees. Uncleaned packaging: Recommendation: Disposal must be made according to official regulations. Recommended cleansing agents: Water, if necessary together with cleansing agents. * SECTION 14: Transport information 14.1 UN-Number Not hazardous for transportation ADR, ADN, IMDG, IATA 14.2 UN proper shipping name None ADR, ADN, IMDG, IATA 14.3 Transport hazard class(es) None ADR, IMDG, IATA Class ADN ADN/R Class: 14.4 Packing group None ADR, IMDG, IATA Not regulated (Contd. on page 6)

22 Page 6/7 Trade name: 5X Colorless GoTaq Reaction Buffer 14.5 Environmental hazards: Marine pollutant: 14.6 Special precautions for user Not applicable Transport in bulk according to Annex II of Marpol and the IBC Code UN "Model Regulation": No Not applicable. (Contd. of page 5) * SECTION 15: Regulatory information 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture Directive 2012/18/EU Named dangerous substances - ANNEX I None of the ingredients is listed. National regulations Classification according to VbF: Waterhazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water Chemical safety assessment: A Chemical Safety Assessment has not been carried out. SECTION 16: Other information This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship. Department issuing MSDS: Promega Corporation Safety Department 2800 Woods Hollow Road Madison, WI U.S.A. Contact: Promega UK Delta House, Enterprise Road Chilworth Science Park Southampton SO16 7NS UNITED KINGDOM Free Phone Free Fax Abbreviations and acronyms: RID: Règlement international concernant le transport des marchandises dangereuses par chemin de fer (Regulations Concerning the International Transport of Dangerous Goods by Rail) ICAO: International Civil Aviation Organisation ADR: Accord européen sur le transport des marchandises dangereuses par Route (European Agreement concerning the International Carriage of Dangerous Goods by Road) IMDG: International Maritime Code for Dangerous Goods IATA: International Air Transport Association GHS: Globally Harmonised System of Classification and Labelling of Chemicals EINECS: European Inventory of Existing Commercial Chemical Substances ELINCS: European List of Notified Chemical Substances CAS: Chemical Abstracts Service (division of the American Chemical Society) VbF: Verordnung über brennbare Flüssigkeiten, Österreich (Ordinance on the storage of combustible liquids, Austria) LC50: Lethal concentration, 50 percent LD50: Lethal dose, 50 percent (Contd. on page 7)

23 Page 7/7 Trade name: 5X Colorless GoTaq Reaction Buffer PBT: Persistent, Bioaccumulative and Toxic vpvb: very Persistent and very Bioaccumulative * Data compared to the previous version altered. (Contd. of page 6)