PPLICATION NOTE DETERMINATION OF PAH

Transcription

1 DETERMIN OF PAH via GPC with On-line EVAporation and HPLC-FLD Meas urement APPLIKS As of: Mai 2015, Version: 1.11

2 Determination of PAH via GPC with on-line EVAporation and HPLC-FLD-measurement Principle of the Method The extracted samples, i.e. food, are handled automatically byy the FREESTYLE GPC. They are processed via the on-line evaporation in the EVAporation module and filled into an HPLC vial ready for injection. Procedure The extracted, dried and filtered raw extract is adjusted to ml and placed on the FREESTYLE system in a 16 ml vial that is closed with a cap/septum. The sample is processed on the system using the method shown in the report on page 4. The description of the process in brief: 5.0 ml are applied by the calibrated sample loop on the GPC column. c The matrix gets separated and the main run with the PAH is already concentrated on-linee in the EVAporation module, that means while the processs in the GPCC is still running. After the evaporation an automated solvent exchange to Acetonitrile/1% 1-Butanol takes place. Finally the EVAporation chamber is rinsed, the concentrate is precisely adjusted to 1.0 ml and filled into an HPLC vial. The measurement of the analytes happens on Thermo 3000 Ultimate U with a fluorescence detector. 2

3 Devices and Materials 1. FREESTYLE Basic P/N FREESTYLE EVAporation P/N FREESTYLE GPC P/N FREESTYLE Direct Injection P/N GPC-Column P/N GPC Frame for rack P/N Rack for 16 ml vials P/N Frame for rack P/N Rack for GC vials P/N ml- vials P/N F016 (100 pcs/pck) 11. Screw cap for 16 ml vialss P/N V0016-SL (100 pcs/pck) 12. Seals P/N V0016-D (100 pcs/pck) ) 13. GC vials P/N V0001 (100 pcs/pck) 14. Crimp cap for GC-vials P/N V0001-B (100 pcs/pck) with seal 15. Cooler P/N 12060, 230 VAC, V 50 Hz 16. Ethyl acetate/cyclohexane for the analysis of organic trace compounds 17. Aceton p.a. 18. Acetonitrile p..a. with 1 % 1-Butanol 19. Standard laboratory glassware and -apparatus On-line connection from GPC directly intoo the EVAporation chamber - possibility of precise concentration up to 0.2 ml. 3

4 Parameterisation of the Method on the FREESTYLE -System 4

5 APPLIC Results without HPLC Direct Injection Module The process time of a sample including solvent exchange and transfer into a GC vial is just 1 h 35 min. Remark: The process time takes at least 10 minutes less, if the sample is measured in a GC and a solvent exchange isn t necessary Recovery rates of an exemplary experiment (n=3) Description Recovery Rate Benzoanthracene/Chrysene 91 ± 4 % Benzo(b)fluoranthene 90 ± 4 % Benzo(a)pyrene 85 ± 5 % Benzochrysene 83 ± 8 % 350 mv Benzo(a)pyrene 270/430 nm Benzochrysene 200 Benzoanthracene/Chrysene Benzo(b)fluoranthene min The figure shows the overlay of two fluorescence chromatograms: external PAH standard (red) and a finished sample (black). 5

6 Parameterisation of the Method on the FREESTYLE -System with HPLC Direct Injection Module 6

7 APPLIC Results with HPLC Direct Injection Module The process time of a sample including solvent exchange and transfer directly into the HPLC system via FREESTYLE Direct Injection module is just 1 h 45 min (process time FREESTYLE ). The processing of the sample is done fully automatically from raw extract to the chromatogram without any manual working step. Recovery rates of an exemplary experiment (n=3) Description Recovery Rate Benzoanthracene/Chrysene 94 ± 2 % Benzo(b)fluoranthene 93 ± 3 % Benzo(a)pyrene 93 ± 2 % Benzochrysene 94 ± 2 % The figure shows the overlay of two fluorescence chromatograms: external PAH standard (red) and a finished sample (black). 7

8 APPLIC Contact LCTech GmbH Bahnweg 41 D Dorfen Germany Tel.: Fax: