Using Single Nucleotide Polymorphism (SNP) to Predict Bitter Tasting Ability
|
|
- Derrick Oliver
- 6 years ago
- Views:
Transcription
1 Using Single Nucleotide Polymorphism (SNP) to Predict Bitter Tasting Ability Part II:! Digestion and Analysis of an Amplified Region of the Bitter Taste Receptor TAS2R38 Gene
2 In The Last Lab:! You sampled some of your own DNA and amplified a short region of chromosome 7.! This region codes for part of TAS2R38 gene, bitter taste receptor gene.
3 In Today s Lab:! You will digest some of your amplified PCR product with the restriction enzyme, Hae III! And use agarose gel electrophoresis to determine your genotype! You will also taste a sample of PTC paper and determine your phenotype! You will compare the results of your genotype with those of your phenotype record on board! Finally, you will use class data to determine if the class is in Hardy-Weinberg equilibrium
4
5 Digestion of TAS2R38 With Restriction Enzyme, Hae III
6 Agarose Gel Electrophoresis
7 Digesting PCR Sample with HAE III Get your PCR tube from your instructor or TA (need your code)! Transfer 16 µl (0.016 ml) of your PCR product (amplified DNA) to a flat top PCR tube! Label this tube D for digested and add your code.! This DNA sample will be digested with the restriction enzyme, HaeIII! Ask your instructor or TA to add 1µl (0.001 ml) of the restriction enzyme, HaeIII, to the D tube! Place this tube in the thermal cycler set for 37 o C and incubate for at least 30 minutes. Label your original PCR tube with a U for undigested; leave this sample on ice while the other sample is incubated with HaeIII.! (choose restriction endonucleases)
8 Agarose Gel Electrophoresis of Amplified PCR Products!You will share an agarose gel (2%) with 2 other people!load both the undigested and digested samples in separate wells of the gel!undigested - 10 µl!digested - 16 µl! Also load the Marker, a 100bp ladder, in both end lanes of the gel!
9 Agarose Gel Run the gel for minutes at 130 Volts; remove gel and stain it with ethidium bromide
10 Staining DNA - Ethidium Bromide!Bring your gel to your TA who will transfer it to the plastic container and add ethidium bromide stain to the DNA bands!ethidium bromide intercalates between the base pairs of the DNA ladder!stain the gel ~20 minutes!destain ~ minutes!view gel using UV light to make the bands fluoresce!analyze photo; attach it to your worksheet
11 Determining Your Genotype Choose RFLP-restriction fragment length polymorphism
12 Determining your PTC Phenotype! Taste the Control paper first (no PTC)! Taste the PTC paper! Place strip in your mouth for a few seconds! Remove it and wait; it takes a few seconds to taste PTC! Record both your genotype and phenotype on board! You will need these results to complete the post-lab assignment
13 Class Results Genotype TT Phenotype strong taster Phenotype weak taster Phenotype non-taster Tt tt * Record the class results on your worksheet.
14 Worksheet! Do sample calculations! Calculate expected allele and genotype frequencies using the sample data given:! T/T: 15! T/t: 50! t/t: 35! Do Chi square calculation to determine if the sample population is in Hardy- Weinberg equilibrium (12minutes) Personal DNA Testing
15 Next Lab DUE: C-fern Lab Report Pre-lab on RPI-LMS: Genetics & Medicine Simulation Lab: Sickle Cell Alleles Quiz: Using SNPs to Predict Bitter Tasting Ability
16 !! How well does TAS2R38 genotype predict PTC-tasting phenotype? What does this tell you about classical dominant/recessive inheritance? The presence of a T allele generally predicts tasting, although heterozygotes are more likely to be weak tasters. Even in a relatively simple genetic system such as PTC tasting, one allele rarely has complete dominance over another. This experiment examined only one of several mutations in the TAS2R38 gene that influence bitter tasting ability. Variability in taste perception is likely affected by processing in the brain, which involves numerous other genes. (E.G., There are about genes that code for bitter taste receptors. Also the genes for three of the cranial nerves involved in taste perception.) Thus, perception of bitter tasting substances like PTC is complex and does not exactly follow the simple Mendelian inheritance pattern.
17 The forward primer incorporates part of the HaeIII recognition site, GGCC. How is this different from the sequence of the human TAS2R38 gene? Mismatch between forward primer & alleles
18 Formation of HaeIII Recongition Site in Amplified PCR Product
19
20
21
22
23 Making sense of SNPs and the Albuterol Story TeachingTools/MakingSNPsMakeSense.html
PTC PCR II: Restriction Enzymes & Gel Electrophoresis
PTC PCR II: Restriction Enzymes & Gel Electrophoresis Objective To apply what we ve learned about genetics, molecular biology, and recombinant DNA to a specific human genetic trait. Background Mammals
More informationGenetics module. DNA Structure, Replication. The Genetic Code; Transcription and Translation. Principles of Heredity; Gene Mapping
Genetics module Lectures DNA Structure, Replication The Genetic Code; Transcription and Translation Principles of Heredity; Gene Mapping Controlling Gene Expression Mutation and Cancer Textbook: Introduction
More informationSTUDENT LAB INSTRUCTIONS
Using a Single-Nucleotide Polymorphism to Predict Bitter-Tasting Ability 5 STUDENT LAB INSTRUCTIONS INTRODUCTION Mammals are believed to distinguish only five basic tastes: sweet, sour, bitter, salty,
More informationStudent Name: Prepared by Dr. Elizabeth Tattersall, Instructor of Biophysical Sciences. WNC-Douglas Campus. Edited by
1 Using A SNP to Predict the Ability to Taste PTC Student Name: Course: Date: Section: Sign-Off: Prepared by Dr. Elizabeth Tattersall, Instructor of Biophysical Sciences WNC-Douglas Campus Edited by Dr.
More informationUsing a Single-Nucleotide Polymorphism to Predict Bitter-Tasting Ability
Using a Single-Nucleotide Polymorphism to Predict Bitter-Tasting Ability 21-1376 21-1377 21-1378 21-1379 21-1380 21-1381 Using a Single-Nucleotide Polymorphism to Predict Bitter-Tasting Ability IMPORTANT
More informationBiology 423L Nov. 6/7. Genetics in Forensic Science: Human DNA Fingerprinting Report due Nov. 21
1 Biology 423L Nov. 6/7 Genetics in Forensic Science: Human DNA Fingerprinting Report due Nov. 21 Readings: Hartwell et al. pp. 297-302, 374-387. Nakamura Y., Carlson, K. Krapco, and R. White 1988. Isolation
More informationminipcr Learning Lab TM : PTC Taster Lab From Genotype to Phenotype
minipcr Learning Lab TM : PTC Taster Lab From Genotype to Phenotype minipcr Learning Labs TM PTC Taster Lab Student s Guide 1 minipcr Learning Lab TM : PTC Taster Lab From Genotype to Phenotype Student
More informationBackground Information 2. Experiment Overview 7. Experiment Procedures 8. Experiment Results and Analysis 12. Related Products 12
Exploring the Genetics of Taste: SNP Analysis of the PTC Gene Using PCR EDVOTEK WORKSHOP Introduction Explore the relationship between genotype and phenotype using Phenylthiocarbamide (PTC). Some think
More informationCat# M6010: A Taste of Genetics, Student Worksheet. Introduction
Cat# M6010: A Taste of Genetics, Student Worksheet Introduction Every human individual perceives the world in a slightly different way. Some differences are due to our past experiences, but some are related
More informationThe Techniques of Molecular Biology: Forensic DNA Fingerprinting
Revised Fall 2016 The Techniques of Molecular Biology: Forensic DNA Fingerprinting **Lab coat, eye goggles and gloves (nitrile or latex) are required for this lab. You will not be allowed to participate
More informationGenetic testing of Tay-Sachs disease by PCR and Restriction Digest
Genetic testing of Tay-Sachs disease by PCR and Restriction Digest ESC102-PRA0103 Submitted to: Elizabeth Berndl 18 February, 2009 Submitted by: Laila Hulbert (996625077) Maria Yancheva (996742173) Scientific
More informationPuro. Knockout Detection (KOD) Kit
Puro Knockout Detection (KOD) Kit Cat. No. CC-03 18 Oct. 2016 Contents I. Kit Contents and Storage II. Product Overview III. Methods Experimental Outline Genomic DNA Preparation Obtain Hybrid DNA Digest
More informationExploring the Genetics of Taste: SNP Analysis of the PTC Gene Using PCR
REVISED & UPDATED Edvo-Kit #345 Exploring the Genetics of Taste: SNP Analysis of the PTC Gene Using PCR Experiment Objective: The objective of this experiment is for students to isolate human DNA and use
More informationminipcr Learning Lab TM : PTC Taster Lab From Genotype to Phenotype
minipcr Learning Lab TM : PTC Taster Lab From Genotype to Phenotype Study questions - Pre-lab 1. Genetics of PTC sensitivity PTC, or 1. phenylthiocarbamide, was discovered accidentally in a lab by the
More informationBio 121 LAB 11 INSTRUCTIONS - DNA II
Bio 121 LAB 11 INSTRUCTIONS - DNA II In the first part of today's lab we will demonstrate that the DNA which we extracted last week can create heritable changes in the phenotype of bacterial cells. We
More informationAmplifying the ALU intron for Hardy- Weinberg Analysis Part 1
Bio 212 Lab Name: Amplifying the ALU intron for Hardy- Weinberg Analysis Part 1 OBJECTIVES: Review the following terms and concepts presented in Biology 211: enzymes, DNA structure and replication, role
More informationSingle Nucleotide Polymorphisms: Measuring the Frequency of Genetic Drift in Populations Written by Greg Dasso Upward Bound 2010
Single Nucleotide Polymorphisms: Measuring the Frequency of Genetic Drift in Populations Written by Greg Dasso Upward Bound 2010 Introduction We ve all seen television shows like CSI where an analyst injects
More informationLaboratory Exercise 4. Multiplex PCR of Short Tandem Repeats and Vertical Polyacrylamide Gel Electrophoresis.
Laboratory Exercise 4 4 Multiplex PCR of Short Tandem Repeats and Vertical Polyacrylamide Gel Electrophoresis B A C K G R O U N D The human genome contains over 3000 million base pairs, which are distributed
More informationminipcr Learning Lab TM : PTC Taster Lab From Genotype to Phenotype
minipcr Learning Lab TM : PTC Taster Lab From Genotype to Phenotype minipcr Learning Labs TM PTC Taster Lab Instructor s Guide 1 minipcr Learning Lab TM : PTC Taster Lab From Genotype to Phenotype Instructor
More informationGenomic Sequencing. Genomic Sequencing. Maj Gen (R) Suhaib Ahmed, HI (M)
Maj Gen (R) Suhaib Ahmed, HI (M) The process of determining the sequence of an unknown DNA is called sequencing. There are many approaches for DNA sequencing. In the last couple of decades automated Sanger
More informationPCR Amplification of The Human Dimorphic Alu PV92 Site 3/17 Honors Biomedical Science 2 Redwood High School Name: [ETRLMBR]
PCR Amplification of The Human Dimorphic Alu PV92 Site 3/17 Honors Biomedical Science 2 Redwood High School Name: [ETRLMBR] Background T he human genome (the total sum of our genetic makeup) is made up
More informationPositive control kit for enzymatic mismatch cleavage and agarose gel visualization (version 2.4)
28 January, 2010 Positive control kit for enzymatic mismatch cleavage and agarose gel visualization (version 2.4) Plant Breeding Unit Brad Till and Owen Huynh January, 2010 Kit Contents: Genomic DNA from
More informationUsing a Single-Nucleotide Polymorphism to Predict Bitter-Tasting Ability
Using a Single-Nucleotide Polymorphism to Predict Bitter-Tasting Ability 21-1376 21-1377 21-1378 21-1379 21-1380 21-1381 Using a Single-Nucleotide Polymorphism to Predict Bitter-Tasting Ability IMPORTANT
More informationFMF NIRCA PROTOCOL STEP 1.
FMF NIRCA PROTOCOL STEP 1. After you have isolated patient s DNA and DNA from a healthy donor (wild type), you perform a nested PCR. The primers used to amplify exon 2 and exon 10 of the mefv gene are
More informationLABORATORY 8. POPULATION GENETICS AND EVOLUTION
STUDENT GUIDE LABORATORY 8. POPULATION GENETICS AND EVOLUTION Objectives In this activity, you will learn about the Hardy-Weinberg law of genetic equilibrium study the relationship between evolution and
More informationAP BIOLOGY Population Genetics and Evolution Lab
AP BIOLOGY Population Genetics and Evolution Lab In 1908 G.H. Hardy and W. Weinberg independently suggested a scheme whereby evolution could be viewed as changes in the frequency of alleles in a population
More informationExercise 20 GEL ELECTROPHORESIS OF DNA SAMPLES (Plasmids, PCR products & Restriction Fragments)
Exercise 20 GEL ELECTROPHORESIS OF DNA SAMPLES (Plasmids, PCR products & Restriction Fragments) Introduction Gel electrophoresis is a technique or procedure allowing DNA fragments to be separated on the
More informationConcepts: What are RFLPs and how do they act like genetic marker loci?
Restriction Fragment Length Polymorphisms (RFLPs) -1 Readings: Griffiths et al: 7th Edition: Ch. 12 pp. 384-386; Ch.13 pp404-407 8th Edition: pp. 364-366 Assigned Problems: 8th Ch. 11: 32, 34, 38-39 7th
More information..C C C T C A T T C A T T C A T T C A T T C A..
Polymerase Chain Reaction Lab: a Forensic Application INTRODUCTION PCR (polymerase chain reaction) is a technique that scientists use to amplify particular segments of DNA. This process can produce large
More informationOverview. Background ~30 min. Lab activity ~50 min. DNA profiling Polymerase Chain Reaction (PCR) Gel Electrophoresis PCR
Overview Day 1: Tuesday Introduction to DNA profiling How do we use DNA to solve crimes? Background Polymerase Chain Reaction (PCR) Gel Electrophoresis Set up PCR Day 2: Wednesday Make and Run Agarose
More informationBIOLOGY - CLUTCH CH.20 - BIOTECHNOLOGY.
!! www.clutchprep.com CONCEPT: DNA CLONING DNA cloning is a technique that inserts a foreign gene into a living host to replicate the gene and produce gene products. Transformation the process by which
More informationGenetic characterization and polymorphism detection of casein genes in Egyptian sheep breeds
Genetic characterization and polymorphism detection of casein genes in Egyptian sheep breeds Othman E. Othman and Samia A. El-Fiky Cell Biology Department - National Research Center - Dokki - Egypt Corresponding
More informationGenetic Diagnosis. electrophoresis. During the lab, genetic testing was done for the cystic fibrosis gene in a young
Meyers 1 Keya Meyers Genetic Diagnosis Abstract: In this lab two processes were observed: restriction fragment polymorphism and gel electrophoresis. During the lab, genetic testing was done for the cystic
More informationPractical 4: PCR in Molecular Diagnosis
PRINCIPLES What is PCR Practical 4: PCR in Molecular Diagnosis Instructors: Dr. Valerie C.L. Lin and Dr. Sze Chun Chau PCR stands for polymerase chain reaction. The PCR method was devised and named by
More informationTexas A&M University-Corpus Christi CHEM4402 Biochemistry II Laboratory Laboratory 8: DNA Restriction Digest (II) and DNA Sequencing (I)
Texas A&M University-Corpus Christi CHEM4402 Biochemistry II Laboratory Laboratory 8: DNA Restriction Digest (II) and DNA Sequencing (I) We have made considerable progress in our analysis of the gene for
More informationBISC110/S11: Lab 6 Taster SNP1
BISC110/S11: Lab 6 Taster SNP1 From OpenWetWare Contents 1 Introduction 2 Lab 6 - Taster SNP Week 1 3 Assignments 4 Link to the Labs in this Series & in the Photosynthesis Series Introduction In the 1930
More informationIdentification of Unknown Plasmid Code Named 681A18
Identification of Unknown Plasmid Code Named 681A18 By Cody Latham Plasmids are small circular, doublestranded DNA molecules commonly found in bacteria that are separate from the chromosomal DNA found
More informationLab 5: Shark Attacks, Again! DNA Fingerprinting to the Rescue
Lab 5: Shark Attacks, Again! DNA Fingerprinting to the Rescue Notebook Lab Objectives Develop an understanding of the basic techniques used to study genetic polymorphisms encoded in DNA Gain familiarity
More informationIntroduction to some aspects of molecular genetics
Introduction to some aspects of molecular genetics Julius van der Werf (partly based on notes from Margaret Katz) University of New England, Armidale, Australia Genetic and Physical maps of the genome...
More informationLecture Four. Molecular Approaches I: Nucleic Acids
Lecture Four. Molecular Approaches I: Nucleic Acids I. Recombinant DNA and Gene Cloning Recombinant DNA is DNA that has been created artificially. DNA from two or more sources is incorporated into a single
More informationMidterm 1 Results. Midterm 1 Akey/ Fields Median Number of Students. Exam Score
Midterm 1 Results 10 Midterm 1 Akey/ Fields Median - 69 8 Number of Students 6 4 2 0 21 26 31 36 41 46 51 56 61 66 71 76 81 86 91 96 101 Exam Score Quick review of where we left off Parental type: the
More informationCONCEPTS AND METHODS INSTRUCTOR PLANNING AND PREPARATION
CONCEPTS AND METHODS This laboratory can help students understand several important concepts of modern biology: The relationship between genotype and phenotype. The use of transposable elements to mutagenize
More informationLesson 3 Gel Electrophoresis of Amplified PCR Samples and Staining of Agarose Gels
Lesson 3 Gel Electrophoresis of Amplified PCR Samples and Staining of Agarose Gels What Are You Looking At? Before you analyze your PCR products, let s take a look at the target sequence being explored.
More informationThe Techniques of Molecular Biology: Forensic DNA Fingerprinting
The Techniques of Molecular Biology: Forensic DNA Fingerprinting Revised Fall 2017 Laboratory Safety: Lab coat, long pants, closed-toe shoes, safety goggles, and nitrile or latex gloves are required. Learning
More informationB. Incorrect! Ligation is also a necessary step for cloning.
Genetics - Problem Drill 15: The Techniques in Molecular Genetics No. 1 of 10 1. Which of the following is not part of the normal process of cloning recombinant DNA in bacteria? (A) Restriction endonuclease
More informationPCR Laboratory Exercise
PCR Laboratory Exercise Advance Protocol (updated 1/2018) Introduction Detection of TPA-25 Alu by PCR A Human DNA Fingerprinting Lab Protocol 1994 Cold Spring Harbor Laboratory DNA Learning Center In this
More informationBENG 183 Trey Ideker. Genotyping. To be covered in one 1.5 hr lecture
BENG 183 Trey Ideker Genotyping To be covered in one 1.5 hr lecture Genetic variation: Some basic definitions Allele Alternative form of a genetic locus inherited separately from each parent Polymorphism
More informationGENOTYPING BY PCR PROTOCOL FORM MUTANT MOUSE REGIONAL RESOURCE CENTER North America, International
Please provide the following information required for genetic analysis of your mutant mice. Please fill in form electronically by tabbing through the text fields. The first 2 pages are protected with gray
More informationpamp, pkan, or pblu?
pamp, pkan, or pblu? Introduction In my final biotechnology lab project, I was given an unidentified plasmid, in which I was required to perform restriction digests using restriction enzymes. A plasmid,
More informationCSS451 Spring 2010 Polymerase Chain Reaction Laboratory
CSS451 Spring 2010 Polymerase Chain Reaction Laboratory The purpose of the polymerase chain reaction (PCR) is to amplify specific segments of DNA. If one knows the DNA sequence of regions of DNA that flank
More informationBiology 445K Winter 2007 DNA Fingerprinting
Biology 445K Winter 2007 DNA Fingerprinting For Friday 3/9 lab: in your lab notebook write out (in bullet style NOT paragraph style) the steps for BOTH the check cell DNA prep and the hair follicle DNA
More informationC. Incorrect! Second Law: Law of Independent Assortment - Genes for different traits sort independently of one another in the formation of gametes.
OAT Biology - Problem Drill 20: Chromosomes and Genetic Technology Question No. 1 of 10 Instructions: (1) Read the problem and answer choices carefully, (2) Work the problems on paper as needed, (3) Pick
More informationHLA-DR TYPING OF GENOMIC DNA
HLA-DR TYPING OF GENOMIC DNA Zofia SZCZERKOWSKA, Joanna WYSOCKA Institute of Forensic Medicine, Medical University, Gdañsk, Poland ABSTRACT: Advances in molecular biology techniques allowed for introduction
More informationLab 8: Population Genetics and Evolution. This may leave a bad taste in your mouth
Lab 8: Population Genetics and Evolution This may leave a bad taste in your mouth Pre-Lab Orientation Recall that the Hardy-Weinberg Equation helps us identify allele frequencies throughout a population.
More informationUser Manual. Version 5. Published February Catalog No. K1021 ~
GeneFishing TM DEG Premix Kit User Manual Version 5 Published February 2005 Catalog No. K1021 ~ 1026 Table of Contents 1. Notices to Customers 1.1 Product Warranty and Liability------------------------------------
More informationLecture 8: Sequencing and SNP. Sept 15, 2006
Lecture 8: Sequencing and SNP Sept 15, 2006 Announcements Random questioning during literature discussion sessions starts next week for real! Schedule changes Moved QTL lecture up Removed landscape genetics
More informationLAB ACTIVITY ONE POPULATION GENETICS AND EVOLUTION 2017
OVERVIEW In this lab you will: 1. learn about the Hardy-Weinberg law of genetic equilibrium, and 2. study the relationship between evolution and changes in allele frequency by using your class to represent
More informationMission (Im)possible: Plasmid Mapping Student Materials
Mission (Im)possible: Plasmid Mapping Student Materials Introduction... 2 Pre-Lab Questions... 6 Lab Protocol... 7 Data Collection Worksheet... 11 Post-Lab Questions and Analysis... 12 Last updated: August
More informationI. Gene Cloning & Recombinant DNA. Biotechnology: Figure 1: Restriction Enzyme Activity. Restriction Enzyme:
I. Gene Cloning & Recombinant DNA Biotechnology: Figure 1: Restriction Enzyme Activity Restriction Enzyme: Most restriction enzymes recognize a single short base sequence, or Restriction Site. Restriction
More informationBS 50 Genetics and Genomics Week of Nov 29
BS 50 Genetics and Genomics Week of Nov 29 Additional Practice Problems for Section Problem 1. A linear piece of DNA is digested with restriction enzymes EcoRI and HinDIII, and the products are separated
More informationActivity 4.3.2: Hypercholesterolemia
Activity 4.3.2: Hypercholesterolemia Introduction In the previous activity, you learned that Anna Garcia has abnormally high cholesterol levels. Because of this result, Anna was sent back to the lab for
More informationDNA FINGERPRINTING VIA THE PCR HUMAN Alu INSERTION POLYMORPHISM
1 DNA FINGERPRINTING VIA THE PCR HUMAN Alu INSERTION POLYMORPHISM Revised s10 Objectives Understand the technique of the PCR Extract, amplify, and analyze human DNA Observe genetic variation among individuals
More informationHOW MANY CATs? A DNA Profiling Simulation
HOW MANY CATs? A DNA Profiling Simulation Background Information 1. Structure of DNA Double helix Anti-parallel strands 4 Bases (A, C, G, and T) Complementary bases Template Strand 5 3 A T T G A C 3 T
More informationHiPer Random Amplification of Polymorphic DNA (RAPD) Teaching Kit
HiPer Random Amplification of Polymorphic DNA (RAPD) Teaching Kit Product Code: HTBM031 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 3.5 hours Agarose Gel Electrophoresis:
More informationAh, Lou! There really are differences between us!
Name Per Ah, Lou! There really are differences between us! Maria C. Abilock BABEC Frank H. Stephenson, Ph.D. Applied Biosystems We gratefully acknowledge David Micklos of the DNA Learning Center at Cold
More informationGenome Sequence Assembly
Genome Sequence Assembly Learning Goals: Introduce the field of bioinformatics Familiarize the student with performing sequence alignments Understand the assembly process in genome sequencing Introduction:
More informationGenotype vs. Phenotype: Genetics of Taste Catalog # 3101
GenoSensor Corporation Genotype vs. Phenotype: Genetics of Taste Catalog # 3101 Version C July 2016 User Manual Genotype vs. Phenotype: Genetics of Taste Kit Manual Table of Contents Notes for Instructors...
More informationGenotype vs. Phenotype: Genetics of Taste Catalog # 3101
GenoSensor Corporation Genotype vs. Phenotype: Genetics of Taste Catalog # 3101 Version E September 2018 User Manual Genotype vs. Phenotype: Genetics of Taste Kit Manual Table of Contents Notes for Instructors...
More informationRestriction Analysis of Purified para-r
Restriction Analysis of Purified para-r INTRODUCTION The restriction analysis will provide final proof that the cells transformed during Laboratory 6, cloned overnight in LB/amp and purified in Lab 10
More informationLab 9 Restriction Enzyme Analysis
Name Assignment # Lab 9 Restriction Enzyme Analysis http://www.phschool.com/science/biology_place/labbench/lab6/concepts2.html 1) Define restriction enzyme 2) Define recognition sequence 3) Label the images
More informationChapter 10 (Part II) Gene Isolation and Manipulation
Biology 234 J. G. Doheny Chapter 10 (Part II) Gene Isolation and Manipulation Practice Questions: Answer the following questions with one or two sentences. 1. What does PCR stand for? 2. What does the
More informationMission (Im)possible: Determine the Identity of Unknown Plasmids. Student Materials. Introduction Lab Protocol... 5
Mission (Im)possible: Determine the Identity of Unknown Plasmids Student Materials Introduction... 2 Lab Protocol... 5 Data Collection Worksheet... 9 Pre-Lab Questions... 10 Post-Lab Questions and Analysis...
More informationBotanoTech. S c ience N o t ebook. Comparative Plant Genomics Study
BotanoTech S c ience N o t ebook Comparative Plant Genomics Study Table of Contents Introduction & Question... 2 Page Research Phenotype...... 3 Hypothesis. 4 Materials & Methods. 5 Part I Genomic DNA
More informationBIO 202 Midterm Exam Winter 2007
BIO 202 Midterm Exam Winter 2007 Mario Chevrette Lectures 10-14 : Question 1 (1 point) Which of the following statements is incorrect. a) In contrast to prokaryotic DNA, eukaryotic DNA contains many repetitive
More informationAh, Lou! There really are differences between us!
Name Per Ah, Lou! There really are differences between us! Introduction The human genome (the total sum of our genetic makeup) is made up of approximately 6 billion base pairs distributed on 46 chromosomes.
More informationDNA amplification and analysis: minipcr TM Food Safety Lab
Science for everyone, everywhere DNA amplification and analysis: minipcr TM Food Safety Lab Release date: 09 September 2014 Welcome Our goals for today: Review DNA amplification theory Solve a public health
More informationRFLP ANALYSIS OF DNA LABORATORY
RFLP ANALYSIS OF DNA LABORATORY BIG PICTURE You will be working with an essential research method widely used in genetics, conservation biology, and forensics. The lab is divided into three sections. Part
More informationRestriction Site Mapping:
Restriction Site Mapping: In making genomic library the DNA is cut with rare cutting enzymes and large fragments of the size of 100,000 to 1000, 000bp. They are ligated to vectors such as Pacmid or YAC
More informationExplain why the scientists used the same restriction endonuclease enzymes on each DNA sample
Q1.Some populations of flies are becoming resistant to insecticides intended to kill them. Scientists developed a method for finding out whether a fly was carrying a recessive allele, r, that gives resistance
More informationApplication of Biotechnology in DNA Fingerprinting and Forensic Analysis. Copyright 2009 Pearson Education, Inc.
Application of Biotechnology in DNA Fingerprinting and Forensic Analysis Introduction to DNA Fingerprinting and Forensics Forensic science intersection of law and science Historic examples Early 1900s
More informationii State two types of evidence left at the scene of the crime which may have been used to provide the DNA sample.
1 The diagram below shows the results of a test which can be used to analyse evidence left at the scene of a crime. This can then be compared with samples taken from various suspects. a i Name this technique.
More informationMolecular Biology (2)
Molecular Biology (2) Restriction endonucleases, RFLP, and gene cloning Mamoun Ahram, PhD Second semester, 2017-2018 Resources This lecture Cooper, pp 120-124 Endonucleases Enzymes that degrade DNA within
More informationData Sheet Quick PCR Cloning Kit
Data Sheet Quick PCR Cloning Kit 6044 Cornerstone Ct. West, Ste. E DESCRIPTION: The Quick PCR Cloning Kit is a simple and highly efficient method to insert any gene or DNA fragment into a vector, without
More informationGel Electrophoresis: Quantitative length and mass measurements of DNA
BIO440 Genetics Lab Humboldt State University Gel Electrophoresis: Quantitative length and mass measurements of DNA Electrophoresis, and in particular agarose gel electrophoresis, is an integral analysis
More informationSuperiorScript III cdna Synthesis Kit Instruction Manual
SuperiorScript III cdna Synthesis Kit Instruction Manual Cat.# EZ405S, EZ405M SuperiorScript III cdna Synthesis Kit Table of Contents I. Description... 3 II. Kit... 4 III. Procedure... 5 IV. Control Experiment
More informationShort Tandam Repeat (D1S58) Detection Kit (for Academic Instructions) Product # 54600
3430 Schmon Parkway Thorold, ON, Canada L2V 4Y6 Phone: 866-667-4362 (905) 227-8848 Fax: (905) 227-1061 Email: techsupport@norgenbiotek.com Short Tandam Repeat (D1S58) Detection Kit (for Academic Instructions)
More informationAh, Lou! There really are differences between us!
Name Ah, Lou! There really are differences between us! Maria C. Abilock BABEC Frank H. Stephenson, Ph.D. Applied Biosystems We gratefully acknowledge David Micklos of the DNA Learning Center at Cold Spring
More informationminipcr Forensics Lab: Analysis of the D1S80 VNTR
minipcr Forensics Lab: Analysis of the D1S80 VNTR Student s Guide Contents 1. Scenario overview p 2 2. Laboratory guide p 15 3. Study questions p 22 1 m i n i P C R L e a r n i n g L a b s - H u m a n
More informationManipulating DNA. Nucleic acids are chemically different from other macromolecules such as proteins and carbohydrates.
Lesson Overview 14.3 Studying the Human Genome Nucleic acids are chemically different from other macromolecules such as proteins and carbohydrates. Nucleic acids are chemically different from other macromolecules
More informationObjectives Introduction restriction endonucleases Examples: Hind III: Eco RI: Pst I:
Objectives Before doing this lab you should understand how gel electrophoresis separates DNA molecules present in a mixture and how restriction endonucleases function. After doing this lab you should be
More information7.1 Techniques for Producing and Analyzing DNA. SBI4U Ms. Ho-Lau
7.1 Techniques for Producing and Analyzing DNA SBI4U Ms. Ho-Lau What is Biotechnology? From Merriam-Webster: the manipulation of living organisms or their components to produce useful usually commercial
More informationDNA Restriction Digestion Analysis
PR041 G-Biosciences 1-800-628-7730 1-314-991-6034 technical@gbiosciences.com A Geno Technology, Inc. (USA) brand name DNA Restriction Digestion Analysis Teacher s Guidebook (Cat. # BE 307) think proteins!
More informationApplication of Molecular Biology tools for cloning of a foreign gene
IFM/Kemi Linköpings Universitet September 2013/LGM Labmanual Project course Application of Molecular Biology tools for cloning of a foreign gene Table of contents Introduction... 3 Amplification of a gene
More informationDNA FINGERPRINTING & OTTER POPULATIONS
DNA FINGERPRINTING & OTTER POPULATIONS 1 DNA FINGERPRINTING AND OTTER POPULATIONS DNA, or deoxyribonucleic acid, is found in all living organisms. DNA is a long chain of nucleotides, the order of which
More informationGENETICS EXAM 3 FALL a) is a technique that allows you to separate nucleic acids (DNA or RNA) by size.
Student Name: All questions are worth 5 pts. each. GENETICS EXAM 3 FALL 2004 1. a) is a technique that allows you to separate nucleic acids (DNA or RNA) by size. b) Name one of the materials (of the two
More informationAuthors: Vivek Sharma and Ram Kunwar
Molecular markers types and applications A genetic marker is a gene or known DNA sequence on a chromosome that can be used to identify individuals or species. Why we need Molecular Markers There will be
More informationminipcr TM Genes in Space Food Safety Lab: Mars Colony at Risk!
minipcr TM Genes in Space Food Safety Lab: Mars Colony at Risk! An E. coli outbreak affects astronaut food aboard the International Space Station. DNA samples from two food racks are analyzed to determine
More informationMolecular Markers CRITFC Genetics Workshop December 9, 2014
Molecular Markers CRITFC Genetics Workshop December 9, 2014 Molecular Markers Tools that allow us to collect information about an individual, a population, or a species Application in fisheries mating
More informationMCB 102 University of California, Berkeley August 11 13, Problem Set 8
MCB 102 University of California, Berkeley August 11 13, 2009 Isabelle Philipp Handout Problem Set 8 The answer key will be posted by Tuesday August 11. Try to solve the problem sets always first without
More informationMicrosatellite markers
Microsatellite markers Review of repetitive sequences 25% 45% 8% 21% 13% 3% Mobile genetic elements: = dispersed repeat included: transposition: moving in the form of DNA by element coding for transposases.
More information