GUIDELINES FOR PRETRANSFUSION TESTING : PART I
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1 Commissie voor Klinische Biologie Commission de Biologie Clinique GUIDELINES FOR PRETRANSFUSION TESTING : PART I Dr. A. Vanhonsebrouck, Bloedtransfusiecentrum Antwerpen WIV congress Rue Juliette Wytsmanstraat Brussels Belgium T F info@iph.fgov.be
2 INTRODUCTION 1.PURPOSE OF GUIDELINES : PROVIDE SAFE TRANFUSION PRACTICE -technical/clerical errors -non-validated techniques equipment -non-compliance with procedures may lead to HEMOLYTIC TRANSFUSION REACTION 2. BASIS -experts from transfusion services and recommendations from haemovigilance reports -UK : Guidelines BCSH -USA : Guidelines AABB -France : Arrêté du 26 avril 2002 : bonnes pratiques d immuno-hémato -Nederland : CBO, Richtlijn bloedtransfusie -Belgium : HGR : 8085, haemovigilance reports, nomenclature Art 24
3 INTRODUCTION 3. ELEMENTS IN PRETRANSFUSION TESTING -I. SAMPLE LABELLING -II. ABO/Rh D GROUPING -III. ANTIBODY SCREENING -IV. ANTIBODY IDENTIFICATION -V. SELECTION OF RED CELL PRODUCTS FOR TRANSFUSION (in case of positive antibody identification) -VI. CROSSMATCH -VII. TYPE AND SCREEN
4 INTRODUCTION 4. QUALITY ASSURANCE IN PRETRANSFUSION PROCEDURES LAB: QUALITY MANAGEMENT SYSTEM -procedures -participation in external/internal QC -reagents equipment techniques -staff training and proficiency -information system /automated equipment : validation HTC : HOSPITAL TRANSFUSION COMMITTEE -policies,procedures for administration of blood (request forms, taking - labelling samples, storage administration of blood) -report transfusion incidents
5 I. SAMPLE LABELLING -request form sample : minimum patient identification items surname name date of birth unique hospital number -patient identity direct inquiry :asking the patient to state his/hername inspection of wrist band /identification label -labelling immediately after /at the moment the blood sample is drawn -unlabelled samples shall be discarded
6 II. ABO AND Rh D GROUPING -most important serological test -ABO grouping cell grouping : monoclonal anti-a, anti-b reagents reverse grouping : A1 and B reagent red cells -D grouping cell grouping : monoclonal; not detect category D VI (exception : NEONATES : detection of weak/partial D VI) -manual : 2 determinations 2 technologists -2 techniques -automation : 1 determination - 1 technologist -1 technique -FINAL blood group : determined on 2 separate independent samples BLOOD GROUP CARD (except Neonates : > 4 (6)M)
7 III. ANTIBODY SCREENING REAGENT RED CELLS 3 group O screening cells with specific antigen composition (C,c,D,E,e/ K,k/Fya,b/Jka,b/SsMN/Le,b/P1 (18 Ag) (homozygous Fya,b/Jka,b/Ss) (R1R1,R2R2) TECHNIQUES -IAT 37 C (reference method) : LISS-IAT 37 C reference method for detecting clinically significant red cell antibodies -sensitivity : column agglutination solid phase technique higher than tube technique -specificity : tube technique is higher than column agglutination/solid phase technique -ENZYME 37 C (not recommended)
8 IV. ANTIBODY IDENTIFICATION (1) REAGENT RED CELLS : at least 10 cells of group O -these cells must fulfill specific criteria of antigen composition to detect all clinically significant antibodies AUTOCONTROL ASSIGNING SPECIFICITY -reactive with at least 2 ex. of reagent red cells carrying the antigen -non reactive with at least 2 ex. of reagent red cells lacking the antigen CHECKING PATIENT ON ABSENCE OF THE CORRESPONDING ANTIGEN EXCLUDE UNDERLYING ANTIBODIES (second panel)
9 IV. ANTIBODY IDENTIFICATION (2) TECHNIQUES -IAT 37 C best method for detection of clinically significant antibodies -ENZYME 37 C helpfull in antibody identification in case of : mixture of antibodies weak reactive antibodies in IAT
10 V. SELECTION OF RED CELLS FOR TRANSFUSION (1) Specificity Clinical Significance Selection of units Rh antibodies YES Antigen negative Kell antibodies YES Antigen negative Duffy antibodies YES Antigen negative Kidd antibodies YES Antigen negative Anti-S,s, YES Antigen negative Anti-M (37 C) Sometimes Antigen negative Anti-M Rarely IAT XM compatible 37 C
11 V. SELECTION OF RED CELLS FOR TRANSFUSION (2) Specificity Clinical Significance Selection of units Anti-Le a, Le b,ant Le a+b Rarely IAT XM compatible 37 C Anti-A1,P1,N Rarely IAT XM compatible 37 C Anti-Lu a Rarely IAT XM compatible 37 C Anti-Lu b Yes Ag negative HTLA Unlikely ask advice transf.centre Antibodies low/frequency antigens Depends on specificity ask advice transf.centre
12 VI. CROSSMATCH (1) -ABO /Rh D group of patient : determined twice before selecting the units (same isogroup) for crossmatch -ABO, Rh D of selected units are compared with blood group of patient (iso group or compatible) -TECHNIQUE : IAT 37 C (irregular antibodies) TUBE SALINE (ELECTRONIC CROSSMATCH) -validity : 72 h -standardisation for all specimens for pretransfusion -only obligatory for patients with Transfus/Pregnancy<3 M -arbitrary safe interval related to the production of unexpected antibodies
13 VI. CROSSMATCH (2) -NEONATUS Group O Rh D dependent on Rh D of neonatus In case of maternal antibody : selected unit is negative for corresponding antigen sample of the mother ( neonatus ) until 4 months of age technique : IAT -CROSSMATCH for WOMEN < 45 year Compatible for Rh D antigen and K antigen (other Rh antigens)
14 VII. TYPE AND SCREEN (1) NOT FOR -Intrauterine transfusions (IUT) -Neonatus until 3 (4-6) months of age -Patients with alloantibodies (autoantibodies) -Organ transplantation (until 3 months after) -Allogeneic stem cell transplantation
15 VII. TYPE AND SCREEN (2) -ABO and Rh D type of patient are performed 2 determinations are determined, one on a current sample -antibody screening is performed and is negative -validity is 72 h -lab must assure validity ABO /Rh D group on donor unit -computerized matching with donorunitnumber, component name, ABO group Rh D type, confirmed ABO donor unit group recipient ABO group, antibody screen result
16 VIII. DAT -DAT is not required as a part of pretransfusion testing. -DAT or AUTOCONTROL : required in case of antibody identification -POLYSPECIFIC ANTISERA : Screening -MONOSPECIFIC ANTISERA : Identification and ELUTION -CLINIC : AIHA, HDN, HEMOLYTIC TRANSFUSION REACTION
17 VIII. REFERENCES 1. UK (BCSH) : Guidelines for compatibility procedures in blood transfusion laboratories Tranf. Med.2004,14,59-73 F.J. Chapman et al 2. UK (BCSH) : Guidelines for pretransfusion compatibility procedures in blood transfusion laboratories Transf. Med.1996,6, UK (Red Book) : Guidelines for the Blood Transfusion Services 4. European Guide : Guide to the use, preparation and quality control of blood components, 14 ed, Council of Europe, 2008 Recommendation n R95(15) 5. UK : Clinical Significance of blood group antibodies G. Daniels et al Transf. Med 2002,12, UK : UK United EQ Assessment Scheme (Blood transfusion Practice): trends in Profiency and practice between , S. Knowles et al Transf.Med 2002,12, UK Comparison of performance of microtube column systems and solidphase systems and tube low-ionic strength solution additive indirect antiglobulin test in the detection of red cell antibodies V. Weisbach et al. Transf.Med,2006,16, AABB Technical Manual : 14th edition, Pretransfusion Testing 9. France : Arrêté du 26 avril 2002: Bonne pratiques d immuno-hémato 10. Netherlands : CBO Richtlijn Bloedtransfusie 11. Belgium : Hemovigilantieverslag 2007, Belgium : Guidelines for the transfusion of red cells, HGR Belgium : RIZIV /INAMI Nomenclature of Clinical Biology
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