Attached Growth Biological Systems
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1 Pendahuluan Biological Wastewater treatment Nur Hidayat Aerob Pasif Aktif Anaerob Pasif Aktif Attached Growth Biological Systems Trickling Filters Biotowers Rotating Biological Contactors Wetlands, Leach Fields, Land-based systems Aerobic Biological Systems Bacteria grow on fixed media (like rocks, plastic, teeth) Heterotrophic bacteria consume soluble BOD Nitrifiers consume NBOD if conditions right Biofilm Schematic Outer portion of biofilm aerobic If film gets too thick then inner portion can go anaerobic When microorganisms near media surface die, lose ability to cling to surface and biofilm sloughed off New biofilm grows Trickling Filters Use tables of HLR and OLR to determine Volume and Area. Check both at both high and low values of range Use Eckenfelder or Velz equation to determine effluent concentration (Ce) 1
2 Trickling Filters A bed of highly permeable medium to which microorganisms attach Wastewater trickled through medium (rotating arm or sprinkler system) to maintain aerobic environment Underdrain collects treated wastewater and detached film (ventilation system if needed) Settling tank needed (often effluent (not sludge) recycled to trickling filter) Rotating Biological Contactors (RBCs) Bacteria grow on plastic disks on rotating shafts Bacteria encounter alternating exposure to air and wastewater as it rotates. ACTIVATED SLUDGE PROCESS SECONDARY TREATMENT Biological Wastewater Treatment Microorganisms consume organic matter from the wastewater, using oxygen for respiration O 2 O 2 O 2 Millions of aerobic and facultative micro-organisms remove pollutants thru living and growing process 2
3 New Cells Wastewater Activated Sludge System Oxygen Food Storage Slime Layer Cell Membrane Enzymes NH 3 CO 2 H 2 O Pri.Eff. Air Provides Oxygen and Mixing Biomass (suspended) Aeration Tank MLSS Clarifier Sec. Eff. (Absorption) Soluble Organics Adsorbed Particle Return Activated Sludge (RAS) Waste Activated Sludge (WAS) Primary Effluent Mixed Liquor (MLSS) Clarifier Return Sludge Aeration Tank Land Application Influent Screening Grit Sludge Processing and Storage Primary Clarifiers WAS RAS Aeration Tanks Typical Flow-Through Activated Sludge Plant Disinfect Effluent Clarifiers Biological Wastewater Treatment Three Steps 1. Transfer of Food from Wastewater to Cell. Adequate Mixing Enough Detention Time Biological Wastewater Treatment 2. Conversion of Food to New Cells and Byproducts. Acclimated Biomass Useable Food Supply Adequate D.O. Proper Nutrient Balance 100 : 5 : 1 C : N : P 3
4 Biological Wastewater Treatment Biological Wastewater Treatment 3. Flocculation and Solids Removal Proper Mixing Proper Growth Environment Clarification 3. Flocculation and Solids Removal Must Have Controls Proper Growth Environment Filamentous Bacteria Form Strings Mixed Liquor Does Not Compact - Bulking Control Factors Activated Sludge System Organic Load, F:M Pri.Eff. Air Biomass Quantity and Age Aeration Tank D.O. Return Activated Sludge MLSS Hydraulic Load Solids Load Clarifier Sec. Eff. Settleability Sludge Blanket Depth Organic Load Pri.Eff. = Pounds of Organics (BOD) Coming into Aeration Tank Aeration Tank MLSS Clarifier Sec. Eff. Waste Activated Sludge OXYGEN DEMAND Biochemical Oxygen Demand B.O.D. The Quantity of Oxygen Used in the Biochemical Oxidation of Organic Material. 5 Day Test OXYGEN DEMAND Biochemical Oxygen Demand B.O.D. Best to Use a Moving Average to Determine the Average Impact on a Treatment System. 5 Day Test 4
5 Date BOD Moving Average Calculate the 7 day moving average of pounds of BOD for 10/5 and 10/6. Pounds of BOD 9/ / / / / / / / / / ,525 13,525 7 = /6 13, ,821 12,821 7 = 1832 Need to Balance Organic Load (lbs BOD) With Number of Active Organisms in Treatment System Ratio Food to Microorganism F F:M or M How Much Food? Primary Effluent BOD Lbs/D BOD = FLOW (MGD) X 8.34 Lbs/Gal X P.E. BOD (mg/l) F = Pounds BOD (Coming into Aeration Tank) How is M (Microorganisms) measured? Mixed Liquor Suspended Solids (MLSS) and Mixed Liquor Volatile Suspended Solids (MLVSS) Mixed Liquor Volatile Suspended Solids (MLVSS) M = Pounds MLVSS (In Aeration Tank) Mixed Liquor Suspended Solids (MLSS) and Mixed Liquor Volatile Suspended Solids (MLVSS) Solids Determining MLSS Wt. of Solids + Paper, mg Wt. of Paper, mg Wt. of Solids, mg Wt. of Solids, mg Volume of Sample, L MLSS, mg/l 5
6 Determining MLVSS Solids Volatile Solids 550 o C How Much Food (F)? Pounds BOD Lbs/D BOD = FLOW (MGD) X 8.34 Lbs/Gal X Pri. Eff. BOD (mg/l) Wt. of Volatile Solids, mg Volume of Sample, L Wt. of Dish + Solids, mg Wt. of Dish + Ash, mg Wt. of Volatile Solids, mg MLVSS, mg/l How is M (Microorganisms) measured? Mixed Liquor Volatile Suspended Solids (MLVSS) M = Pounds MLVSS Food to Microorganism Ratio Food to Microorganism Ratio The F/M Ratio for Best Treatment Will Vary for Different Facilities Determined by Regular Monitoring and Comparing to Effluent Quality Often Will Vary Seasonally F M = = Lbs of BOD Lbs of MLVSS Typical Range: Conventional Activated Sludge F:M Extended Aeration Activated Sludge F:M Calculate Often to Monitor/Control Monthly (Minimum) Weekly (Better) Use Moving Average Anaerobic wastewater treatment Sedimentasi floc-forming bacteria dan recycling Sedimentation and suspension of particles formed from bacteria and minerals inside the bioreactor Immobilizing the bacteria at the surface of either fixed or suspended solid materials, as well as at rotating solid plates High-rate reactor designs Anaerobic digester designs based on biomass retention: (a) anaerobic filter/fixed bed reactor; (b) downflow stationary fixedfilm reactor; (c) expanded bed/fluidised bed reactor; (d) upflow anaerobic sludge blanket reactor; Expanded granular Sludge Bed (e) hybrid sludge bed/fixed bed reactor 6
7 Contact Processes In the anaerobic contact process, the higher bacterial concentration is most often obtained in an incompletely mixed tank reactor (suspended growth) as a result of recycling sludge after settling in a sedimentation tank (Fig. 8.5), similar to an activated sludge reactor Contact Processes The reactor is mixed using: stirrers, the distribution of recycled external wastewater near the reactor bottom, the recycling of compressed biogas (not presented in Fig. 8.5). Upflow Anaerobic Sludge Blanket In the upflow anaerobic sludge blanket (UASB) type of reactor, the gas/solid/liquid separation system is integrated into the vessel (Fig. 8.6). This reactor can only be used if large, dense, readily settleable particles are formed a granular sludge which allows high concentrations of suspended solids between 20 g/l MLSS and 30 g/l MLSS The influent wastewater is distributed at the bottom by a system of tubes which provides a flow through a blanket of granular sludge. Inside these porous particles, fatty acids and biogas are formed. The reaction rate of the process is controlled by diffusion, convection and reaction inside the pores. Ascending biogas bubbles keep the particles partially fluidized. Depending on the kind of wastewater, the sludge tends to be more or less flocculating, which determines how well it is suspended in the fluidized sludge The biogas contains sulphide, which can be removed in iron filters (FeS precipitation) A UASB reactor for the treatment of 6000 PE (person equivalents) domestic wastewater 7
8 Anaerobic ponds Also called lagoons (in the US) or waste stabilisation ponds Low-rate anaerobic process (e.g. 1 2 kgcod/m 3 /d) Solids settling and anaerobic decomposition Depth: 5-10 m Could be covered for odour control and gas collection (but most of them are not covered) Usually several ponds in series (last pond: aerobic maturation pond with algae; pathogen kill by sunlight) Anaerobic ponds Influent (faecal sludge, greywater or conventional ww.) Sludge layer (increasing over time) Effluent This slide was provided by Peter van der Steen (UNESCO-IHE) This pond is not covered A sludge crust may form and act as a cover Limitations of anaerobic ponds Large land area required - Potentially high costs for covers, if these are used Needs desludging after years (this is often forgotten!) - e.g. By stopping inflow, then settling and drying for 2 months then manual emptying reuse in agriculture? Feed flow distribution inefficiencies Poor contact between substrates and biomass (see schematic below) Effluent Influent Substrates Sludge layer (biomass) The issue of methane emissions and covering of ponds Anaerobic ponds emit biogas which contains the greenhouse gas methane It is possible to cover the ponds and to collect the biogas (for energy generation or flaring) Floating cover systems: floating membrane made of lined PVC or High Density Polyethylene - Covers needs to be durable, UV protected, chemically resistent to biogas; support foot traffic and rainwater loads New: emission reduction contracts can be signed based on capturing the methane gas from anaerobic lagoons sale of biogas emission reduction possible - First example in developing country: Santa Cruz, Bolivia in 2006 (Source: Menahem Libhaber in Huber s Symposium Water Supply and Sanitation for All, Sept. 2007, Berching, Germany) Are you aware of anaerobic ponds (waste stabilisation ponds/lagoons) in your city? Could they be covered? Anaerobic digestion process overview In the anaerobic digestion process, micro-organisms convert complex organic matter to biogas, which consists of methane (CH 4 ) and carbon dioxide (CO 2 ) Some organic matter remains even after the digestion step, and this is called digestate or digester residue or digested organic matter Anaerobic digestion is used to treat high-strength wastewater, organic solid waste, sewage sludges, blackwater, faecal sludge, agricultural waste, food industry waste (e.g. breweries, slaughter houses, dairy), manure,... Anaerobic digestion with biogas production also occurs in landfills, septic tanks, cows rumen, natural or constructed wetlands, dams where vegetation was flooded all these sites produce methane gas! Organic matter (energy-rich) Anaerobic digestion process schematic Example: Liquid flowrate: 10 m 3 /d Mass flowrate: 1 ton VS/d Biogas (methane): Green energy Example: Gas flowrate: 665 Nm 3 /d * Anaerobic digester (biological reactor) * Calculated by using 0.95 Nm 3 /kg VS destroyed - see next slide Digestate (energy-poor; can be used as fertiliser; includes anaerobic biomass) Liquid flowrate: 10 m 3 /d Mass flowrate: 0.3 ton VS/d Nm 3 stands for normal cubic metre, meaning a measurement at STP or standard temperature and pressure (absolute pressure of 100 kpa (1 bar) and a temperature of K (0 C)) 8
9 Anaerobic digestion (AD) microbiology fundamentals Under anaerobic conditions, organic substances are not aerated (oxidised), but are fermented (reduced) (Reduction = assimilation of electrons) Energy-rich end products, like organic acids or alcohols are electron acceptors It is quite a slow process (low growth rate of methanogens) compared to aerobic processes relatively long sludge retention times are required Like all biological processes, it is temperature dependent (higher conversion rates at higher temperatures) digesters are typically heated / insulated or below ground The process occurs as a four-step process (see next slide) 4 steps in anaerobic conversion Remember: this is not a complete conversion - some organic matter will remain (digestate) Note that biogas is a mixture not only the useful CH 4 Depending on the substrate there 50can be other gases too (slide 27) 9
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