Quant Reverse Transcriptase
|
|
- Joel Benson
- 6 years ago
- Views:
Transcription
1 1. Quant Reverse Transcriptase For first-strand cdna synthesis and two-step RT-PCR
2 RT Kit Contents Quant Reverse Transcriptase Contents Cat. no. ER103 ER rxns ER rxns ER rxns Quant Reverse Transcriptase 25 μl 50 μl 2 50 μl 10 RT Buffer 70 μl 150 μl μl Handbook Storage Quant Reverse Transcriptase should be shipped on dry ice and stored immediately upon receipt at 20 C. Introduction Quant Reverse Transcriptase is a new, unique enzyme, different from the reverse transcriptases of Moloney Murine Leukemia Virus (MMLV) or Avian Myeloblastosis Virus (AMV). Quant Reverse Transcriptase is a recombinant heterodimeric enzyme expressed in E. coli. It has a high affinity for RNA, which enables efficient and sensitive reverse transcription of RNA templates with high GC content or complex secondary structures, leading to high yields of cdna. Application RT-PCR, Real-Time PCR, 3 and 5 RACE PCR, prime extension, cdna library construction, serial analysis of gene expression (SAGE), etc. Quant Reverse Transcriptase Handbook 1
3 Important Notes 1. The protocol is optimized for use with 50 ng to 2 μg of RNA. With >2 μg RNA, scale up the reaction linearly to the appropriate volume. 2. Set up all reactions on ice to minimize the risk of RNA degradation. 3. Separate denaturation and annealing steps are generally not necessary. However, for some RNAs with a high degree of secondary structure, a denaturation step may be desired. If so, denature the RNA in RNase-free water before reaction setup: incubate the RNA for 5 min at 65 C, then place immediately on ice. Do not denature the RNA in the reaction mix. 4. When using oligo-dt primers, a primer length of at least 12 nucleotides and a final concentration of 1 μm is recommended. 5. Operate carefully to minimize RNA degradation and cross contamination. Starting Template Reverse transcriptases are used in vitro for first-strand cdna synthesis with RNA as the starting template. The efficiency of the reaction is highly dependent on the quality and quantity of the starting RNA template. 1. It is important to have intact RNA as starting template. Even trace amounts of contaminating RNases in the RNA sample can cause RNA cleavage, resulting in shortened cdna products. 2. Chemical impurities, such as protein, poly-anions (e.g., heparin), salts, EDTA, ethanol, phenol, and other solvents, can affect the activity and processivity of the reverse ranscriptase. Quant Reverse Transcriptase Handbook 2
4 Protocol The protocol is optimized for use with 50 ng to 2 μg of RNA. With >2 μg RNA, scale up the reaction linearly to the appropriate volume. 1. Thaw template RNA on ice. Thaw the primer solutions, 10x RT Buffer (supplied), Super pure dntp, and RNase-free ddh 2 O at room temperature (15 25 C). Place on ice immediately after thawing. Mix each solution by vortexing, and centrifuge briefly to collect residual liquid from the sides of the tubes. 2. Dilute the RNA inhibitor to final concentration of 10 U/μl in 1x RT Buffer which has been pre-cooled in ice. Mix thoroughly and carefully by vortexing for no more than 5 sec. Centrifuge briefly. 3. Prepare a fresh master mix on ice according to Table 1. Mix thoroughly and carefully by vortexing for no more than 5 sec. Centrifuge briefly and then store on ice. Add the RNA template in the step 5. Note: 10 μl reverse-transcription reaction volume could be set up for downstream qpcr. All the components can be reduced to 1/2 to adapt for SuperReal PreMix (SYBR Green) (FP204-01, 50 μl 50 rxns; FP204-02, 50 μl 200 rxns). 4. If setting up multiple reverse-transcription reactions, distribute the appropriate volume of master mix into individual reaction tubes. Keep tubes on ice. 5. Add the template RNA (50 ng-2 μg) to the individual tubes containing the master mix. Mix thoroughly and carefully by vortexing for no more than 5 sec. Centrifuge briefly. 6. Incubate for 60 min at 37 C. 7. Add an aliquot of the finished reverse-transcription reaction Quant Reverse Transcriptase Handbook 3
5 to the PCR mix. Note: Put the reverse-transcription products on ice first and then apply them in following PCR amplification. For longterm storage, store the products at 20 C. The first strand cdna synthesis reaction mixture should not comprise more than 1/10 of the total PCR reaction volume. Table 1. Reverse-Transcription Reaction Components Component Volume/Reaction Final Concentration 10x RT Buffer 2 μl 1 Super pure dntp (2.5 mm each) 4 μl 0.5 mm each dntp Oligo-dT (10 μm)* 2 μl 1 μm RNA Inhibitor 1 μl 10 U (for 20 μl reaction system) Quant Reverse Transcriptase RNase-free ddh 2 O Template RNA added in the step 5 Total reaction volume 1 μl X μl X μl 20 μl (for 20 μl reaction system) PCR Amplification Two Step RT-PCR In Two-Step RT-PCR, an RNA strand is first reverse transcribed into its DNA complement; the cdna products can then be used for PCR amplification. RT and PCR are preformed separately in different tubes subsequently. Both random primers and gene specific primers can be used in Two-Step RT PCR, according the following Quant Reverse Transcriptase Handbook 4
6 steps: 1. Synthetize the first-strand cdna with RNA as the starting template according to the above procedure. 2. Add the cdna products into PCR reaction system. The first strand cdna synthesis reaction mixture should not comprise more than 1/10 of the total PCR reaction volume. 3. Perform the PCR amplification. One Step RT-PCR One step RT-PCR performs RT as well as PCR in a single buffer system. The reaction proceeds without the addition of reagents between the RT and PCR steps. First, cdna first-strand is amplified at 37 C, at which temperature the activity of Taq DNA polymerase is pretty low. When the reverse transcription is completed, increasing temperature inactivates the reverse transcriptase, and Taq DNA polymerase start to synthetize cdna. Since the RT and PCR are performed in the same tube, the buffer system cannot be optimized separately for these two reactions. Furthermore, reverse transcriptase has low activity in some PCR buffer. Therefore, One Step RT-PCR is not recommended. Quant Reverse Transcriptase Handbook 5
7 Ordering Information RNA Isolation Product Size Cat.no. RNAprep pure Kit (For Cell/Bacteria) 50 preps DP430 RNAprep pure Kit (For Tissue) 50 preps DP431 RNAprep pure Kit (For Plant) 50 preps DP432 Real Time PCR Product Size Cat.no. SuperReal PreMix (SYBR Green) 50 μl 50 rxns 50 μl 200 rxns FP FP Quant Reverse Transcriptase Handbook 6
8 PCR Product Size Cat.no. 2 Taq PCR MasterMix (with loading dye) 1 ml 5 1 ml KT KT Quant Reverse Transcriptase Handbook 7
Quantscript RT Kit. For first-strand cdna synthesis and two step RT-PCR.
1. Quantscript RT Kit For first-strand cdna synthesis and two step RT-PCR www.tiangen.com ER121221 Quantscript RT Kit Cat. no. KR103 Kit Contents Contents KR103-03 25 rxn KR103-04 100 rxn Quant Reverse
More informationQuant One Step RT-PCR Kit
1. Quant One Step RT-PCR Kit For fast and sensitive one-step RT-PCR www.tiangen.com/en RT121221 Quant One Step RT-PCR Kit Kit Contents Cat. no. KR113 Contents Hotmaster Taq Polymerase (2.5 U/μl) Quant
More informationFastFire qpcr PreMix (Probe)
FastFire qpcr PreMix (Probe) For fast, quantitative, specific real-time PCR using sequence-specific probe www.tiangen.com/en FP130820 Kit Contents FastFire qpcr PreMix (Probe) Contents 2 FastFire qpcr
More informationPrimeScript 1st strand cdna Synthesis Kit
Cat. # 6110A For Research Use PrimeScript 1st strand cdna Synthesis Kit Product Manual Table of Contents I. Description... 3 II. Components... 3 III. Materials Required but not Provided... 3 IV. Storage...
More informationRP RXN RTase/RI Enzyme Mix 5X RT Buffer (DTT/dNTPs) Oligo (dt)/random Primer Mix DEPC-Treated H2O
www.smobio.com Product Information Reverse Transcription Kit II RP1400 100 RXN RTase/RI Enzyme Mix 5X RT Buffer (DTT/dNTPs) Oligo (dt)/random Primer Mix DEPC-Treated H2O ExcelRT series 100 μl 500 μl 100
More informationPrimeScript RT Master Mix (Perfect Real Time)
Cat. # RR036A For Research Use PrimeScript RT Master Mix (Perfect Real Time) Product Manual Table of Contents I. Description... 3 II. Kit Components... 3 III. Materials Required but not Provided... 3 IV.
More informationPrimeScript RT reagent Kit (Perfect Real Time)
Cat. # RR037A For Research Use PrimeScript RT reagent Kit (Perfect Real Time) Product Manual Table of Contents I. Description... 3 II. Components... 3 III. Storage... 3 IV. Features... 4 V. Precautions...
More informationHiPer RT-PCR Teaching Kit
HiPer RT-PCR Teaching Kit Product Code: HTBM024 Number of experiments that can be performed: 5 Duration of Experiment: Protocol: 4 hours Agarose Gel Electrophoresis: 45 minutes Storage Instructions: The
More informationAMV First Strand cdna Synthesis Kit
DNA AMPLIFICATION & PCR AMV First Strand cdna Synthesis Kit Instruction Manual NEB #E6550S Store at 20 C ISO 9001 Registered Quality Management ISO 14001 Registered Environmental Management ISO 13485 Registered
More informationTIANamp Soil DNA Kit. For isolation of genomic DNA from soil.
TIANamp Soil DNA Kit For isolation of genomic DNA from soil www.tiangen.com/en DP140311 TIANamp Soil DNA Kit Kit Contents (Spin Column) Cat. no. DP336 Contents Buffer SA Buffer SC Buffer HA Buffer HB Buffer
More informationPlant Genomic DNA Kit
Plant Genomic DNA Kit For purification of total cellular DNA from plant cells www.tiangen.com/en DP130227 Plant Genomic DNA Kit Kit Contents Storage Contents (Spin Column) Cat. no. DP305 DP305-02 (50 preps)
More informationGeneCopoeia TM. All-in-One qpcr Mix For universal quantitative real-time PCR. User Manual
GeneCopoeia TM Expressway to Discovery All-in-One qpcr Mix For universal quantitative real-time PCR Cat. No. AOPR-0200 (200 qpcr reactions) Cat. No. AOPR-0600 (600 qpcr reactions) Cat. No. AOPR-1000 (1000
More informationTIANamp Marine Animals DNA Kit
TIANamp Marine Animals DNA Kit For isolation of genomic DNA from marine animal tissues www.tiangen.com/en DP121221 TIANamp Marine Animals DNA Kit (Spin Column) Cat. no. DP324 Kit Contents Contents DP324-02
More informationHuman TNF qpcr primer pair
Shipping and Storage information Catalog No. Amount Reaction number of 25-μl volume Shipping and Storage Package tube 2 nmol 200 lyophilized powder 1 Information of the target gene and primers Species
More informationP HENIX. PHENIX PCR Enzyme Guide Tools For Life Science Discovery RESEARCH PRODUCTS
PHENIX PCR Enzyme Guide PHENIX offers a broad line of premium quality PCR Enzymes. This PCR Enzyme Guide will help simplify your polymerase selection process. Each DNA Polymerase has different characteristics
More informationOne Step SYBR PrimeScript RT-PCR Kit II (Perfect Real Time)
Cat. # RR086A For Research Use One Step SYBR PrimeScript RT-PCR Kit II Product Manual Table of Contents I. Description...3 II. III. IV. Principle...3 Components...5 Storage...6 V. Features...6 VI. VII.
More informationTIANamp Yeast DNA Kit
TIANamp Yeast DNA Kit For isolation of genomic DNA from yeast cells www.tiangen.com/en DP121221 TIANamp Yeast DNA Kit Kit Contents (Spin Column) Cat. no. DP307 Contents Buffer GA Buffer GB Buffer GD Buffer
More informationmmu-mir-200a-3p Real-time RT-PCR Detection and U6 Calibration Kit User Manual MyBioSource.com Catalog # MBS826230
mmu-mir-200a-3p Real-time RT-PCR Detection and U6 Calibration Kit User Manual Catalog # MBS826230 For the detection and quantification of mirnas mmu-mir-200a-3p normalized by U6 snrna using Real-time RT-PCR
More informationSYBR Green Realtime PCR Master Mix
Instruction manual SYBR Green Realtime PCR Master Mix 0810 F0924K SYBR Green Realtime PCR Master Mix QPK-201T 1 ml x 1 QPK-201 1 ml x 5 Contents [1] Introduction [2] Components [3] Primer design [4] Detection
More informationProtoScript. First Strand cdna Synthesis Kit DNA AMPLIFICATION & PCR. Instruction Manual. NEB #E6300S/L 30/150 reactions Version 2.
DNA AMPLIFICATION & PCR ProtoScript First Strand cdna Synthesis Kit Instruction Manual NEB #E6300S/L 30/150 reactions Version 2.2 11/16 be INSPIRED drive DISCOVERY stay GENUINE This product is intended
More informationMMLV Reverse Transcriptase 1st-Strand cdna Synthesis Kit
MMLV Reverse Transcriptase 1st-Strand cdna Synthesis Kit Cat. No. MM070150 Available exclusively thru Lucigen. lucigen.com/epibio www.lucigen.com MA265E MMLV Reverse Transcriptase 1st-Strand cdna Synthesis
More informationGuide-it sgrna In Vitro Transcription and Screening Systems User Manual
Clontech Laboratories, Inc. Guide-it sgrna In Vitro Transcription and Screening Systems User Manual Cat. Nos. 631438, 631439 & 631440 (042114) Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra
More informationII First Strand cdna Synthesis Kit
DNA AMPLIFICATION & PCR ProtoScript II First Strand cdna Synthesis Kit Instruction Manual NEB #E6560S/L 30/150 reactions Version 1.5 12/17 be INSPIRED drive DISCOVERY stay GENUINE This product is intended
More informationReverse Transcriptase Reverse Transcriptase 100 µl 5X RT Buffer 0.1 M DTT 500 µl Storage -20 C for 24 months
www.smobio.com Product Information Reverse Transcriptase ExcelRT series RP1000 20,000 units Reverse Transcriptase 100 µl 5X RT Buffer 1 ml 0.1 M DTT 500 µl Storage -20 C for 24 months Description The ExcelRT
More informationPremix Ex Taq (Probe qpcr)
For Research Use Premix Ex Taq (Probe qpcr) Product Manual Table of Contents I. Description... 3 II. Principle... 4 III. Components... 5 IV. Materials Required but not Provided... 5 V. Storage... 5 VI.
More informationEpiQuik Quantitative PCR Fast Kit Base Catalog # P-1029
EpiQuik Quantitative PCR Fast Kit Base Catalog # PLEASE READ THIS ENTIRE USER GUIDE BEFORE USE Uses: The EpiQuik Quantitative PCR Fast Kit is designed for quantitative real time analysis of DNA samples
More informationQuantiTect Primer Assay Handbook
July 2011 QuantiTect Primer Assay Handbook For genomewide, ready-to-use real-time RT-PCR assays using SYBR Green detection Sample & Assay Technologies QIAGEN Sample and Assay Technologies QIAGEN is the
More informationSuperScript IV Reverse Transcriptase as a better alternative to AMV-based enzymes
WHITE PAPER SuperScript IV Reverse Transcriptase SuperScript IV Reverse Transcriptase as a better alternative to AMV-based enzymes Abstract Reverse transcriptases (RTs) from avian myeloblastosis virus
More informationFunctional Genomics Research Stream. Research Meeting: June 19, 2012 SYBR Green qpcr, Research Update
Functional Genomics Research Stream Research Meeting: June 19, 2012 SYBR Green qpcr, Research Update Updates Alternate Lab Meeting Fridays 11:30-1:00 WEL 4.224 Welcome to attend either one Lab Log thanks
More informationReverse transcription-pcr (rt-pcr) Dr. Hani Alhadrami
Reverse transcription-pcr (rt-pcr) Dr. Hani Alhadrami hanialhadrami@kau.edu.sa www.hanialhadrami.kau.edu.sa Overview Several techniques are available to detect and analyse RNA. Examples of these techniques
More informationGlobin Block Modules for QuantSeq Instruction Manual
Globin Block Modules for QuantSeq Instruction Manual Catalog Numbers: 070 (RS-Globin Block, Homo sapiens, 96 rxn) 071 (RS-Globin Block, Sus scrofa, 96 rxn) 015 (QuantSeq 3 mrna-seq Library Prep Kit for
More informationReverTra Ace qpcr RT Master Mix
Instruction manual ReverTra Ace qpcr RT Master Mix 1203 F1173K ReverTra Ace qpcr RT Master Mix FSQ-201 200 reactions Store at -20 C Contents [1] Introduction [2] Components [3] Protocol 1. RNA template
More informationFor simultaneous purification of genomic DNA and total RNA from the same animal cells or tissues
1. TIANamp DNA/RNA Isolation Kit For simultaneous purification of genomic DNA and total RNA from the same animal cells or tissues www.tiangen.com/en RP090603 TIANamp DNA/RNA Isolation Kit Kit Contents
More informationmmu-mir-34a Real-time RT-PCR Detection Kit User Manual
mmu-mir-34a Real-time RT-PCR Detection Kit User Manual Catalog # CPK1272 For the detection and quantification of mirna mmu-mir-34a using Real-Time RT-PCR detection instruments. For research use only. Not
More informationMir-X mirna First-Strand Synthesis and SYBR qrt-pcr
User Manual Mir-X mirna First-Strand Synthesis and SYBR qrt-pcr User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories,
More information2x PCR LongNova-RED PCR Master Mix
2x PCR LongNova-RED Components RP85L 100 reactions (50 μl) RP85L-10 1000 reactions (50 μl) 2x PCR LongNova-RED 2 x 1.25 ml 20 x 1.25 ml PCR grade water 2 x 1.5 ml 20 x 1.5 ml Storage & Shiing Storage conditions
More informationFOR RESEARCH USE ONLY. NOT FOR HUMAN OR DIAGNOSTIC USE.
Instruction manual RNA-direct SYBR Green Realtime PCR Master Mix 0810 F0930K RNA-direct SYBR Green Realtime PCR Master Mix Contents QRT-201T QRT-201 0.5mLx2 0.5mLx5 Store at -20 C, protected from light
More informationE.Z.N.A. Microorganism Direct PCR Kit
E.Z.N.A. Microorganism Direct PCR Kit TQ3100-00 TQ3100-01 TQ3100-02 20 preps 100 preps 500 preps June 2013 E.Z.N.A. Microorganism Direct PCR Kit Table of Contents Introduction and Overview...2 Kit Contents/Storage
More informationT7-Based RNA Amplification Protocol (in progress)
T7-Based RNA Amplification Protocol (in progress) Jacqueline Ann Lopez (modifications) Amy Cash & Justen Andrews INTRODUCTION T7 RNA Amplification, a technique originally developed in the laboratory of
More informationTIANgel Mini DNA Purification Kit
TIANgel Mini DNA Purification Kit For DNA purification from agarose and polyacrylamide gels www.tiangen.com/en DP130419 TIANgel Mini DNA Purification Kit Kit Contents (Spin column) Cat. no. DP208 Contents
More informationAbsolute Human Telomere Length Quantification qpcr Assay Kit (AHTLQ) Catalog # reactions
Absolute Human Telomere Length Quantification qpcr Assay Kit (AHTLQ) Catalog #8918 100 reactions Product Description Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect
More informationPhosphate buffered saline (PBS) for washing the cells TE buffer (nuclease-free) ph 7.5 for resuspending the SingleShot RNA control template
Catalog # Description 172-5085 SingleShot SYBR Green Kit, 100 x 50 µl reactions For research purposes only. Introduction The SingleShot SYBR Green Kit prepares genomic DNA (gdna) free RNA directly from
More informationSYBR Premix DimerEraser (Perfect Real Time)
Cat. # RR091A or Research Use SYBR Premix DimerEraser (Perfect Real Time) Product Manual Table of Contents I. Description... 3 II. Principle... 3 III. Components... 4 IV. Storage... 5 V. eatures... 5 VI.
More informationInstructions for Use Life Science Kits & Assays
Instructions for Use Life Science Kits & Assays Content Content 1 Product and order number... I 2 Storage conditions... I 3 Description... II 3.1 Quality data... II 3.2 Unit definition... II 4 Delivered
More informationQUANTITATIVE RT-PCR PROTOCOL (SYBR Green I) (Last Revised: April, 2007)
QUANTITATIVE RT-PCR PROTOCOL (SYBR Green I) (Last Revised: April, 007) Please contact Center for Plant Genomics (CPG) facility manager Hailing Jin (hljin@iastate.edu) regarding questions or corrections.
More informationLaboratory #7 PCR PCR
1 Laboratory #7 Polymerase chain reaction () is DNA replication in a test tube. In vitro enzymatic amplification of a specific segment of DNA. Many Applications. direct cloning from DNA or cdna. Mutagenesis
More informationFMF NIRCA PROTOCOL STEP 1.
FMF NIRCA PROTOCOL STEP 1. After you have isolated patient s DNA and DNA from a healthy donor (wild type), you perform a nested PCR. The primers used to amplify exon 2 and exon 10 of the mefv gene are
More informationDuraScribe T7 Transcription Kit
DuraScribe T7 Transcription Kit Cat. Nos. DS010910 and DS010925 Available exclusively thru Lucigen. lucigen.com/epibio www.lucigen.com MA170E DuraScribe T7 Transcription Kit 7/2017 1 1. Introduction The
More informationShinePolo One Step RT-PCR Kits for qpcr User Manual
ShinePolo One Step RT-PCR Kits for qpcr User Manual Cat.Nos.ZK00102(50 rxns 30ul) Published 24 Feb 2007 ZK00103(100 rxns 30ul) Ⅰ Description ShinePolo One Step RT-PCR Kits is a system for performing qrt-pcr
More informationTable of Contents. PrimeScript TM RT-PCR Kit. I. Kit Contents...2. Storage...3. Principle...4. Features...5. V. Notes...5. Protocol...
Table of Contents I. Kit Contents...2 II. III. IV. Storage...3 Principle...4 Features...5 V. Notes...5 VI. Protocol...6 VII. PCR Condition...8 VIII. Application...8 IX. Preparation of RNA sample...10 X.
More informationPolymerase Chain Reaction (PCR)
Laboratory for Environmental Pathogens Research Department of Environmental Sciences University of Toledo Polymerase Chain Reaction (PCR) Background information The polymerase chain reaction (PCR) is an
More informationRoche Molecular Biochemicals Technical Note No. LC 9/2000
Roche Molecular Biochemicals Technical Note No. LC 9/2000 LightCycler Optimization Strategy Introduction Purpose of this Note Table of Contents The LightCycler system provides different detection formats
More informationQIAGEN LongRange 2Step RT-PCR Handbook
Second Edition December October 2010 2005 QIAGEN LongRange 2Step RT-PCR Handbook For reliable and accurate long-range two-step RT-PCR up to 12.5 kb Sample & Assay Technologies QIAGEN Sample and Assay Technologies
More informationSOP: SYBR Green-based real-time RT-PCR
SOP: SYBR Green-based real-time RT-PCR By Richard Yu Research fellow Centre for Marine Environmental Research and Innovative Technology (MERIT) Department of Biology and Chemistry City University of Hong
More informationSynthetic sirna Quantitation Core Kit
Cat. # 6142 For Research Use Synthetic sirna Quantitation Core Kit Product Manual v201010da Table of Contents I. Description... 3 II. Kit Components... 3 III. Materials Required but not Provided... 4 IV.
More informationTitanium One-Step RT-PCR Kit. User Manual. User Manual
User Manual Titanium One-Step RT-PCR Kit User Manual United States/Canada 800.662.2566 Asia Pacific +1.650.919.7300 Europe +33.(0)1.3904.6880 Japan +81.(0)77.543.6116 Clontech Laboratories, Inc. A Takara
More informationAll-in-One TM mirna qrt-pcr Detection Kit
mirna qrt-pcr Detection Kit For quantitative detection of mature mirna Cat. No. QP015 (20 RT and 200 qpcr reactions) Cat. No. QP016 (60 RT and 600 qpcr reactions) Used in combination with the All-in-One
More informationInstructions for Use Life Science Kits & Assays
Instructions for Use Life Science Kits & Assays Product specifications 1 Product specifications The innumix Standard PCR MasterMix contains all reagents required for routine high throughput PCR amplifications
More informationPRODUCT INFORMATION Long PCR Enzyme Mix #K0182 500 u Lot Exp. 00.0000 Store at -20 C. CERTIFICATE OF ANALYSIS Long PCR Enzyme Mix is functionally tested in PCR amplification of 47.4 kb fragment from lambda
More informationTQ RXN 2X Q-PCR Master Mix (SYBR, no ROX) 1 ml x 2 TQ RXN 2X Q-PCR Master Mix (SYBR, no ROX) 1 ml x 5
www.smobio.com Product Information ExcelTaq series 2X Q-PCR Master Mix (SYBR, no ROX) TQ1100 200 RXN 2X Q-PCR Master Mix (SYBR, no ROX) 1 ml x 2 TQ1101 500 RXN 2X Q-PCR Master Mix (SYBR, no ROX) 1 ml x
More informationIDEAL TM Spike-In RNA Kit. Individual Assays Principle, Workflow and Protocol
IDEAL TM Spike-In RNA Kit Individual Assays Principle, Workflow and Protocol Table of Contents Content Page Working Principle 3 Kit Contents & Storage 4 Additional Equipment and Compatibility 5 Workflow
More informationUSB HotStart-IT. for increased specificity and consistent results. PCR, qpcr and qrt-pcr
USB HotStart-IT for increased specificity and consistent results PCR, qpcr and qrt-pcr USB PCR Reagents Choose USB HotStart-IT products for increased specificity and consistent results. Long and Accurate
More informationQuickExtract RNA Extraction Kit
Cat. Nos. QER09015 and QER090150 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com Lit. # 299 12/2012 1
More informationSYBR Advantage qpcr Premix. User Manual
User Manual SYBR Advantage qpcr Premix User Manual United States/Canada 800.66.566 Asia Pacific +.650.99.7300 Europe +33.(0).3904.6880 Japan +8.(0)77.543.66 Clontech Laboratories, Inc. A Takara Bio Company
More informationQuickExtract FFPE RNA Extraction Kit
QuickExtract FFPE RNA Extraction Kit Cat. Nos. QFR82805 and QFR82050 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com
More informationmirna 1st-Strand cdna Synthesis Kit
mirna 1st-Strand cdna Synthesis Kit INSTRUCTION MANUAL Catalog #600036 Revision B This document contains two instruction manuals for use with Catalog # 600584. See the 600036-12 manual, shown first, for
More informationTable of content. One Step SYBR R PrimeScript TM RT-PCR Kit II (Perfect Real Time) I. Description...2. II. Principle III. Kit Contents...
Table of content I. Description...2 II. Principle... 2 III. Kit Contents...4 IV. Storage...5 V. Features...5 VI. Note...5 VII. Protocol...6 VIII. Experiment Example...11 IX. Appendix...12 X. Guideline
More informationBioBank TM cdna Kit. Instructions for the use of BioBank TM cdna in real-time PCR. BioBank control cdna
BioBank TM cdna Kit Instructions for the use of BioBank TM cdna in real-time PCR BB BioBank control cdna Contents Introduction 3 Kit Contents 4 Reagents and Equipment to Be Supplied by User 4 PrimerDesign
More informationPRODUCT INFORMATION Thermo Scientific Luminaris Color Probe qpcr Master Mix #K0354 For 5000 rxns Lot Exp. Store at -20 C in the dark CERTIFICATE OF ANALYSIS The absence of endo-, exodeoxyribonucleases
More informationRNA/cDNA Quality Assay User Manual
.Clontech Laboratories, Inc. RNA/cDNA Quality Assay User Manual (PR0X3688) Cat. No. 636841 Published 1/19/2011 Clontech Laboratories, Inc. A Takara Bio Company 1290 Terra Bella Avenue, Mountain View, CA
More informationInstructions for Use Life Science Kits & Assays
Instructions for Use Life Science Kits & Assays Product specifications 1 Product specifications The innumix qpcr MasterMix Probe has been developed for fast, highly reproducible real-time PCR and has been
More informationSideStep Lysis and Stabilization Buffer
SideStep Lysis and Stabilization Buffer INSTRUCTION MANUAL Catalog #400900 Revision B.0 For Research Use Only. Not for use in diagnostic procedures. 400900-12 LIMITED PRODUCT WARRANTY This warranty limits
More informationRT 2 Easy First Strand Handbook
March 2011 RT 2 Easy First Strand Handbook For cdna synthesis Sample & Assay Technologies QIAGEN Sample and Assay Technologies QIAGEN is the leading provider of innovative sample and assay technologies,
More informationSYBR Premix Ex Taq II (Tli RNaseH Plus), Bulk
Cat. # RR820L For Research Use SYBR Premix Ex Taq II (Tli RNaseH Plus), Bulk Product Manual Table of Contents I. Description... 3 II. Principle... 3 III. Kit Components... 4 IV. Materials Required but
More informationProduct Name : Simple mirna Detection Kit
Product Name : Simple mirna Detection Kit Code No. : DS700 This product is for research use only Kit Contents This kit provides sufficient reagents to perform 20 reactions for detecting microrna. Components
More informationTechnical Review. Real time PCR
Technical Review Real time PCR Normal PCR: Analyze with agarose gel Normal PCR vs Real time PCR Real-time PCR, also known as quantitative PCR (qpcr) or kinetic PCR Key feature: Used to amplify and simultaneously
More informationPCR Add-on Kit for Illumina Instruction Manual
PCR Add-on Kit for Illumina Instruction Manual Catalog Numbers: 001 (SENSE mrna-seq Library Prep Kit V2 for Illumina) 009 (SENSE Total RNA-Seq Library Prep Kit for Illumina) 015 (QuantSeq 3 mrna-seq Library
More informationSYBR Real-Time PCR Kit
SYBR Real-Time PCR Kit For Amplification and detection of DNA in Quantitative real-time PCR (qpcr). Catalog No. QPG-040/QPG-041/QPG-042/QPG-043 User Manual Table of Contents Kit Contents and Storage...
More informationTaura Syndrome Virus (TSV) RT-PCR Kit
Revision No.: ZJ0001 Issue Date: Aug 28th, 2007 Taura Syndrome Virus (TSV) RT-PCR Kit Cat. No.: AR-0200-03 For use with Conventional PCR Instrument or Real time PCR Instrument User Manual For in vitro
More informationQuant-X One-Step qrt-pcr TB Green Kit User Manual
Takara Bio USA Quant-X One-Step qrt-pcr TB Green Kit User Manual Cat. No. 638317 PT5058-1 (022818) 1290 Terra Bella Avenue, Mountain View, CA 94043, USA U.S. Technical Support: techus@takarabio.com United
More informationCSS451 Spring 2010 Polymerase Chain Reaction Laboratory
CSS451 Spring 2010 Polymerase Chain Reaction Laboratory The purpose of the polymerase chain reaction (PCR) is to amplify specific segments of DNA. If one knows the DNA sequence of regions of DNA that flank
More informationID3EAL Spike-in RNA Kit. Principle, Workflow and Protocol
ID3EAL Spike-in RNA Kit Principle, Workflow and Protocol Table of Contents Content Page Working Principle 3 Kit Contents & Storage 4 Additional Equipment and Compatibility 5 Additional Equipment and Compatibility
More informationUser Guide. Full-Length cdna Amplification Kit. Catalog Numbers: 013 (TeloPrime Full-Length cdna Amplification Kit) 018 (TeloPrime PCR Add-on Kit)
Full-Length cdna Amplification Kit User Guide Catalog Numbers: 013 (TeloPrime Full-Length cdna Amplification Kit) 018 (TeloPrime PCR Add-on Kit) 013UG022V0105 FOR RESEARCH USE ONLY. NOT INTENDED FOR DIAGNOSTIC
More informationMasterPure Plant RNA Purification Kit
MasterPure Plant RNA Purification Kit Cat. Nos. MPR09010 and MPR09100 Connect with Epicentre on our blog (epicentral.blogspot.com), Facebook (facebook.com/epicentrebio), and Twitter (@EpicentreBio). www.epicentre.com
More informationGeneChip Eukaryotic Small Sample Target Labeling Assay Version II *
GENE EXPRESSION MONITORING TECHNICAL NOTE GeneChip Eukaryotic Small Sample Target Labeling Assay Version II * Introduction There is an overwhelming and continuing demand for a well characterized protocol
More informationAmpliScribe T7-Flash Transcription Kit
AmpliScribe T7-Flash Transcription Kit Cat. Nos. ASF3257 and ASF3507 Available exclusively thru Lucigen. lucigen.com/epibio www.lucigen.com MA191E AmpliScribe T7-Flash Transcription Kit 12/2016 1 1. Introduction
More informationPrecision PLUS OneStep qrt-pcr Master Mix. Instructions for use of Primerdesign Precision PLUS OneStep Master Mix for real-time RT-PCR
Precision PLUS OneStep qrt-pcr Master Mix Instructions for use of Primerdesign Precision PLUS OneStep Master Mix for real-time RT-PCR Contents Introduction 3 Kit Contents 5 Recommended Accompanying Products
More informationID3EAL mirna qpcr ASSAY. Principle, Workflow and Protocol
ID3EAL mirna qpcr ASSAY Principle, Workflow and Protocol Table of Contents Content Page Working Principle 3 Kit Contents & Storage 4 Additional Equipment and Compatibility 5 Workflow Overview - Option
More informationHiPer Real-Time PCR Teaching Kit
HiPer Real-Time PCR Teaching Kit Product Code: HTBM032 Number of experiments that can be performed: 10 Duration of Experiment Protocol: 1.5 hours Storage Instructions: The kit is stable for 12 months from
More informationSection 2: Eukaryotic Sample and Array Processing Rev. 3
Section 2: Eukaryotic Sample and Array Processing 701024 Rev. 3 Section 2 Contents Section 2 Eukaryotic Sample and Array Processing Chapter 1 Eukaryotic Target Preparation 2.1.3 Eukaryotic Chapter 2 Eukaryotic
More informationPreAnalytiX Supplementary Protocol
PreAnalytiX Supplementary Protocol Purification of total RNA from microdissected PAXgene Tissue fixed, paraffin-embedded (PFPE) and PAXgene Tissue fixed, cryo-embedded (PFCE) tissues This protocol is for
More informationMeDIP-seq library construction protocol v2 Costello Lab June Notes:
MeDIP-seq library construction protocol v2 Costello Lab June 2010 Notes: A. For all Qiagen gel extraction steps (Qiaquick and MinElute), melt gel slice at 37º C instead of 50º (see Quail et al 2008 Nature
More informationTECHNICAL BULLETIN. SeqPlex DNA Amplification Kit for use with high throughput sequencing technologies. Catalog Number SEQX Storage Temperature 20 C
SeqPlex DNA Amplification Kit for use with high throughput sequencing technologies Catalog Number SEQX Storage Temperature 20 C TECHNICAL BULLETIN Product Description The SeqPlex DNA Amplification Kit
More informationRNA LA PCR Kit (AMV) Ver.1.1
Table of Contents I. Description... 2 II. Kit Components... 2 III. Reagents not supplied in the kit... 3 IV. Equipment required... 3 V. Storage... 3 VI. References... 3 VII. Principle... 4 VIII. Features...
More informationMgCl 2 (25 mm) 1.6 ml 1.6 ml 1.6 ml 1.6 ml
Technical Data Sheet KAPA2G Fast PCR Kit Kit components KK 5008 Product codes KK 5010 KK 5009 KK 5011 KAPA2G Fast DNA polymerase (5 U/μl) 100 U 100 U 250 U 250 U 1. Production Description The KAPA2G Fast
More informationPreparing Samples for Analysis of Small RNA
Preparing Samples for Analysis of Small RNA Topics 3 Introduction 4 Kit Contents and Equipment Checklist 6 Isolate Small RNA by Denaturing PAGE Gel 9 Ligate 5' RNA Adapters 12 Ligate 3' RNA Adapters 15
More informationRift Valley Fever Virus RT-PCR Kit
Revision No.: ZJ0002 Issue Date: Jan 2 nd, 2008 Rift Valley Fever Virus RT-PCR Kit Cat. No.: AR-0116-03 For use with Conventional PCR Instrument or Real time PCR Instrument User Manual For in vitro Diagnostic
More informationCopy number standard curve. for absolute quantification by real-time PCR
Copy number standard curve for absolute quantification by real-time PCR Kit contents Positive control template for standard curve (RED) Template preparation buffer (YELLOW) RNase/DNase free water (WHITE)
More informationPreparing Samples for Digital Gene Expression-Tag Profiling with NlaIII
Preparing Samples for Digital Gene Expression-Tag Profiling with NlaIII FOR RESEARCH ONLY Topics 3 Introduction 5 Kit Contents and Equipment Checklist 8 Isolate mrna and Synthesize First Strand cdna 11
More informationGuidelines for Preventing Contamination of PCR Reference Guidelines for Primer Design Estimation of Primer Melting Temperature
Guidelines for Preventing Contamination of PCR During PCR more than 10 million copies of a template DNA are generated. Therefore, care must be taken to avoid contamination with other templates and amplicons
More information