Advanced Laboratory Testing Ltd
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1 Ltd Boxer House, Unit 4, Newbridge Industrial Estate, Newbridge, Co. Kildare Testing Laboratory Registration number: 315T is accredited by the Irish National Board (INAB) to undertake testing as detailed in the Schedule bearing the Registration Number detailed above, in compliance with the International Standard ISO/IEC 17025: nd Edition General Requirements for the Competence of Testing and Calibration Laboratories (This Certificate must be read in conjunction with the Annexed Schedule of ) Date of award of accreditation: 10:09:2013 Date of last renewal of accreditation: 10:09:2013 Expiry date of this certificate of accreditation: 10:09:2018 This shall remain in force until further notice subject to continuing compliance with INAB accreditation criteria, ISO/IEC and any further requirements specified by the Irish National Board. Manager: Dr Adrienne Duff Chairperson: Issued on 10 September 2013 Mr Tom O Neill Organisations are subject to annual surveillance and are re-assessed every five years. The renewal date on this Certificate confirms the latest date of renewal of accreditation. To confirm the validity of this Certificate, please contact the Irish National Board. INAB is a signatory of the European co-operation for (EA) Multilateral Agreement (MLA) and the International Laboratory Cooperation (ILAC) Mutual Recognition Arrangement, for Testing. Edition 12, 24/10/ T Page 1 of 23
2 Schedule of (Annex to Certificate) ADVANCED LABORATORY TESTING LTD Biological and Chemical Testing Laboratory Initial Registration Date : 10-September-2013 Postal Address: Boxer House (Address of other locations Unit 4 Newbridge Industrial Estate as they apply) Newbridge Co. Kildare Telephone: +353 (45) Contact Name: Facilities: grahamohalloran@altesting.ie Graham O'Halloran Public testing service Edition 12, 24/10/ T Page 2 of 23
3 Schedule of THE IRISH NATIONAL ACCREDITATION BOARD (INAB) is the Irish body for the accreditation of organisations including laboratories. Laboratory accreditation is available to testing and calibration facilities operated by manufacturing organisations, government departments, educational institutions and commercial testing/calibration services. Indeed, any organisation involved in testing, measurement or calibration in any area of technology can seek accreditation for the work it is undertaking. Each accredited laboratory has been assessed by skilled specialist assessors and found to meet criteria which are in compliance with ISO/IEC or ISO/IEC (medical laboratories). Frequent audits, together with periodic inter-laboratory test programmes, ensure that these standards of operation are maintained. Testing and Calibration Categories: : Permanent laboratory calibration and testing where the laboratory is erected on a fixed location for a period expected to be greater than three years. Category B: Category C: Category D: Site calibration and testing that is performed by staff sent out on site by a permanent laboratory that is accredited by the Irish National Board. Site calibration and testing that is performed in a site/mobile laboratory or by staff sent out by such a laboratory, the operation of which is the responsibility of a permanent laboratory accredited by the Irish National Board. Site calibration and testing that is performed on site by individuals and organisations that do not have a permanent calibration/testing laboratory. Testing may be performed using (a) (b) (c) (d) portable test equipment a site laboratory a mobile laboratory or equipment from a mobile or site laboratory Standard Specification or Test Procedure Used: The standard specification or test procedure that is accredited is the issue that is current on the date of the most recent visit, unless otherwise stated. Glossary of Terms Facilities: Public calibration/testing service: Conditionally available for public calibration/testing: Normally not available for public calibration/testing: Commercial operations which actively seek work from others. Established for another primary purpose but, more commonly than not, is available for outside work. Unavailable for public calibration/testing more often than not. Laboratory users wishing to obtain assurance that calibration or test results are reliable and carried out to the Irish National Board criteria should insist on receiving an accredited calibration certificate or test report. Users should contact the laboratory directly to ensure that this scope of accreditation is current. INAB will, on request, verify the status and scope. Edition 12, 24/10/ T Page 3 of 23
4 Microbiological tests on foods Egg and egg Meat and meat Detection of Salmonella spp by selective enrichment technique Detection of Salmonella spp using Solus method MTM001: ISO 6579:2002 (A1:2007) & ISO 6785: 2007 MTM002: Solus Salmonella ELISA test Confirmation of Salmonella spp MTM003: ISO 6579:2002 Detection of Listeria spp by selective enrichment technique MTM004: ISO :1997 (A1:2004) Fish, shellfish and molluscs Fats and oils Soups, broths and sauces Fruit and vegetables Herbs and spices Non-alcoholic beverages Alcoholic beverages Detection of Listeria spp using Solus ELISA method MTM005: Solus Listeria ELISA test Confirmation of Listeria spp MTM006: ISO :1997 (A1:2004) Detection and confirmation of thermotolerant Campylobacter spp by selective enrichment technique Detection and confirmation of E. coli 0157 MTM007: ISO :2006 MTM008: ISO 16654: (other than wine) Ices and desserts Detection and confirmation of E. coli 0157:H7 using Reveal test system MTM009: Reveal test system Nuts and nut, Enumeration of microorganisms: aerobic colony count (30 C for 48 hours) MTM010a: ISO : Additives Pet foods Animal feeds Enumeration of microorganisms: aerobic colony count (30 C for 72 hours) Enterobacteriaceae MTM010b: ISO :2013 MTM011: ISO :2004 Edition 12, 24/10/ T Page 4 of 23
5 Microbiological tests on foods Egg and egg coliforms E. coli MTM012:ISO 4832:2006 MTM013a: ISO :2001 Meat and meat E. coli MTM013b: ISO : Fish, shellfish and molluscs Enumeration and confirmation of coagulase positive staphylococci MTM014: ISO : Fats and oils Soups, broths and sauces Enumeration and confirmation of Bacillus cereus Enumeration of Listeria monocytogenes and Listeria spp MTM015: ISO 7932:2005 MTM016: ISO :1998 (A1:2004) Fruit and vegetables Herbs and spices Pseudomonas spp MTM017: ISO 13720: Non-alcoholic beverages Alcoholic beverages Enumeration and confirmation of Clostridium perfringens MTM018: ISO 7937: (other than wine) Ices and desserts faecal streptococci (enterococci) MTM021: BS 4285:1985 section Nuts and nut, Additives yeasts and moulds in food and swabs with an Aw greater than 0.95 MTM022: ISO : Pet foods Animal feeds Detection of Listeria SPP using ALOA One Day Method MTM036:ALOA ONE DAY TM (Detection) AFNOR Validation Certificate N 0 AES 10/3-09/00 Edition 12, 24/10/ T Page 5 of 23
6 811 Microbiological tests on foods yeasts and moulds in food and swabs with an Aw less than 0.95 MTM023: ISO : Egg and egg Meat and meat Fish, shellfish and molluscs Soups, broths and sauces Fruit and vegetables Herbs and spices Ices and desserts Nuts and nut, Additives Pet foods Animal feeds Edition 12, 24/10/ T Page 6 of 23
7 811 Microbiological tests on foods Enumeration and confirmation of lactic acid bacteria MTM019: ISO 15214:1988 Meat and meat Enumeration and confirmation of Lactobacillus species MTM020: Oxoid manual (online) Meat and meat Enumeration of microorganisms: anaerobic colony count MTM030: Documented inhouse method Detection of coliforms MTM031: ISO 4831:2006 Meat and Meat and Meat game and Detection and confirmation of Cronobacter spp (formally Enterobacter sakazakii) MTM040 Rapid Sakazakii ANOR validation Certificate N BRD 07/22-05/12 Meat and meat Detection and confirmation of E.coli O157 using VIDAS UP E.coli O157 Test MTM035: BIOMERIEUX VIDAS UP E. coli O157 (including H7) (ECPT) AFNOR VALIDATION Certificate N BIO 12/25-05/09 Meat and meat ENUMERATION OF CAMPYLOBACTER SPECIES MTM041: ENUMERATION OF CAMPYLOBACTER SPECIES based on ISO :2006 Edition 12, 24/10/ T Page 7 of 23
8 Microbiological tests on foods Egg and egg Meat and meat, game and Fish, shellfish and molluscs Fats and oils Soups, broths and sauces Fruit and vegetables Herbs and spices Non-alcoholic beverages Alcoholic beverages (other than wine) Ices and desserts Nuts and nut, Additives Pet foods Animal feeds Detection of Salmonella spp using Real-Time PCR Detection of Listeria spp using Real-Time PCR DETECTION OF SALMONELLA SPP BY IBISA RAPID CULTURE METHOD MTM037: BIORAD iq- CheckTM Salmonella II. AFNOR VALIDATION Certificate N BRD 07/06-07/04 MTM038: BIORAD iq- CheckTM Listeria spp. AFNOR VALIDATION Certificate N BRD 07/13-05/07 MTM047: IBISA Selective medium for the detection of Salmonella AFNOR VALIDATION Certificate N AES 10/11 07/11 Edition 12, 24/10/ T Page 8 of 23
9 811 Microbiological tests on foods Meat and meat, game and DETECTION, IDENTIFICATION AND CONFIRMATION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI O26, O45, O103, O111, O121, O145 and O157:H7 (STEC) USING REAL- TIME PCR MTM042A BIORAD iq- CheckTM STEC VirX Real- Time PCR. AOAC Certificate reference: MTM042B iq-checktm STEC SerO Real-Time PCR. AOAC Certificate reference: Testing of surfaces in abattoirs Meat surfaces Product contact surfaces Detection of Listeria SPP using ALOA One Day Method MTM036:ALOA ONE DAY TM(Detection) AFNOR Validation Certificate N0 AES 10/3-09/00 Detection of Salmonella spp using Real-Time PCR MTM037: BIORAD iq- CheckTM Salmonella II. AFNOR VALIDATION Certificate N BRD 07/06-07/04 Detection of Listeria spp using Real-Time PCR MTM038: BIORAD iq- CheckTM Listeria spp. AFNOR VALIDATION Certificate N BRD 07/13-05/07 Edition 12, 24/10/ T Page 9 of 23
10 Testing of surfaces in abattoirs Meat surfaces Product contact surfaces DETECTION, IDENTIFICATION AND CONFIRMATION OF SHIGA TOXIN-PRODUCING ESCHERICHIA COLI O26, O45, O103, O111, O121, O145 and O157:H7 (STEC) USING REAL-TIME PCR MTM042A BIORAD iq- CheckTM STEC VirX Real- Time PCR. AOAC Certificate reference: MTM042B iq-checktm STEC SerO Real-Time PCR. AOAC Certificate reference: USDA FSIS MLG 5B.05 ISO/TS 13136: Testing of surfaces in abattoirs Detection of Salmonella spp by selective enrichment technique MTM001: ISO 6579:2002 (A1:2007) & ISO 6785: Meat surfaces Product contact surfaces Detection of Salmonella spp using Solus method MTM002: Solus Salmonella ELISA test Confirmation of Salmonella spp MTM003: ISO 6579:2002 Detection of Listeria spp MTM 004: ISO :1997 (A1:2004) Detection of Listeria spp using Solus ELISA method MTM 005: Solus Listeria ELISA test Confirmation of Listeria spp MTM006: ISO :1997 (A1:2004) Detection and confirmation of E. coli 0157 MTM008: ISO 1654:2001 Edition 12, 24/10/ T Page 10 of 23
11 Detection and confirmation of E. coli 0157:H7 using Reveal test system Enumeration of aerobic colony count (30 C for 48 hours) MTM009: Reveal test system MTM010a: ISO :2013 Enumeration of aerobic colony count (30 C for 72hours) Enterobacteriaceae coliforms MTM010b: ISO :2013 MTM011: ISO :2004 MTM012: ISO 4832: Testing of surfaces in abattoirs E. coli MTM013a: ISO : Meat surfaces Product contact E. coli MTM013b: ISO :2001 surfaces Enumeration and confirmation of coagulase positive staphylococci MTM014: ISO :1999 Enumeration and confirmation of Bacillus cereus MTM015: ISO 7932: 2005 Enumeration of Listeria monocytogenes and Listeria spp MTM016: ISO :1998 (A1:2004) Pseudomonas spp MTM017: ISO 13720:2010 Enumeration and confirmation of Clostridium perfringens MTM018: ISO 7937:2004 Edition 12, 24/10/ T Page 11 of 23
12 faecal streptococci (enterococci) yeasts and moulds in food and swabs with an Aw greater than 0.95 Detection of Salmonella spp from carcass swabs using a USDA compliant method MTM021: BS 4285:1985 Section 3.11 MTM22: ISO :2008 MTM033: MLG 4.08 Enumeration of coliforms and Escherichia coli in carcass swabs using a USDA compliant method MTM034: Association of Official Analytical Chemists (AOAC) official method Microbiological tests for factory hygiene purposes Surfaces DETECTION OF SALMONELLA SPP BY IBISA RAPID CULTURE METHOD Detection of Salmonella spp by selective enrichment technique Detection of Salmonella spp using Solus method MTM047: IBISA Selective medium for the detection of Salmonella AFNOR VALIDATION Certificate N AES 10/11 07/11 MTM001: ISO 6579:2002 (A1:2007) & ISO 6785: 2007 MTM002: Solus Salmonella ELISA test Confirmation of Salmonella spp MTM003: ISO 6579:2002 Detection of Listeria spp using selective enrichment technique Detection of Listeria spp using Solus ELISA method MTM004: ISO :1997 (A1:2004) MTM005: Solus Listeria ELISA test Edition 12, 24/10/ T Page 12 of 23
13 Confirmation of Listeria spp MTM006: ISO :1997 (A1:2004) Detection and confirmation of E. coli 0157 Detection and confirmation of E. coli 0157:H7 using Reveal test system Enumeration of microorganisms: aerobic colony count (30 C for 48 hours) Enumeration of microorganisms: aerobic colony count (30 C for 72 hours) Enterobacteriaceae coliforms MTM008: ISO 16654:2001 MTM009: Reveal test system MTM010a: ISO :2013 MTM010b: ISO :2013 MTM011: ISO :2004 MTM012: ISO 4832: Microbiological tests for factory hygiene purposes Surfaces E. coli E. coli Enumeration and confirmation of coagulase positive staphylococci Enumeration and confirmation of Bacillus cereus Enumeration of Listeria monocytogenes and Listeria spp MTM013a: ISO :2001 MTM013b: ISO :2001 MTM014: ISO :1999 MTM015: ISO 7932: 2205 MTM016: ISO :1998 (A1:2004) Edition 12, 24/10/ T Page 13 of 23
14 Pseudomonas spp Enumeration and confirmation of Clostridium perfringens faecal streptococci (enterococci) yeasts and moulds in food and swabs with an Aw greater than 0.95 MTM017: ISO 13720:2010 MTM018: ISO 7937:2004 MTM021: BS 4285:1985 Section 3.11 MTM022: ISO :2008 Detection of Listeria SPP using ALOA One Day Method Detection and confirmation of Cronobacter spp (formally Enterobacter sakazakii) MTM036:ALOA ONE DAY TM(Detection) AFNOR Validation Certificate N0 AES 10/3-09/00 MTM040 Rapid Sakazakii ANOR validation Certificate N BRD 07/22-05/12 Detection of Salmonella spp using Real-Time PCR Detection of Listeria spp using Real-Time PCR MTM037: BIORAD iq-check TM Salmonella II. AFNOR VALIDATION Certificate N BRD 07/06-07/04 MTM038: BIORAD iq-check TM Listeria spp. AFNOR VALIDATION Certificate N BRD 07/13-05/07 DETECTION OF SALMONELLA SPP BY IBISA RAPID CULTURE METHOD MTM047: IBISA Selective medium for the detection of Salmonella AFNOR VALIDATION Certificate N AES 10/11 07/11 Edition 12, 24/10/ T Page 14 of 23
15 870 Waters, including effluents.11 Bacteriological condition of potable waters.12 Bacteriological condition of industrial waters (treated, recirculating).14 Bacteriological condition of trade wastes Bacteriological condition of swimming pools and spas Bacteriological condition of environmental waters Enumeration of colony count in water Enumeration and confirmation of coliforms and E. coli in water Enumeration and confirmation of Pseudomonas aeruginosa in water Enumeration and confirmation of enterococci in water Enumeration and confirmation of Clostridium perfringens in water Analysis of water for total viable count (HTM 2030) MTM024: "The Microbiology of Drinking Water" 2012, Part 7 MTM025: "The Microbiology of Drinking Water" 2009, Part 4 MTM026: "The Microbiology of Drinking Water" 2010, Part 8 MTM027: "The Microbiology of Drinking Water" 2012, Part 5 MTM028: "The Microbiology of Drinking Water" 2010, Part 6 MTM029: Health Technical Memorandum 2030, 1997 Edition 12, 24/10/ T Page 15 of 23
16 Chemical Testing Laboratory 751 Chemical Tests on Foods Meat and meat Determination of Nitrate, Nitrite and Salt using Anion Exchange Chromatography 7-275mg/kg NaNO 3 and mg/kg as NaNO 2 by IC Salt: 0.1-6g/100g FCTM005: Dionex application note 112, 1998 ISO 2918(1975) Meat and meat - Determination of Nitrite content ISO 3091 (1975) Meat and meat - Determination of Nitrate content, ISO 1841 (1996) Meat and meat - Determination of Chloride content Meat and meat Nuts and nut, DETERMINATION OF TOTAL FAT AND MOISTURE IN FOODS Range: Moisture % Fat: % Powders % Low dairy % Dairy 10-85% Meat Low 0.1-4% Meat 4-30% Sauce % FCTM006 based on AOAC Official Method ( , Chapter 39 p. 27). Using CEM NMR Analyser. Edition 12, 24/10/ T Page 16 of 23
17 Chemical Testing Laboratory Meat and meat Nuts and nut, DETERMINATION OF ASH IN FOODS Range: g/100g FCTM007 based on AOAC Official method ( , Chapter 9, p. 19) using CEM Microwave Furnace. Meat and meat DETERMINATION OF SODIUM (SALT) IN FOODS Range: g/100g FCTM008 based on AOAC Official Method ( , Chapter 50 p. 65). AOAC Official Method , (Chapter 27 p. 21). Using Atomic Absorption Spectrometry. Nuts and nut, Meat and meat, game and Nuts and nut, DETERMINATION OF TOTAL SUGARS IN FOOD Range: g/100g FCTM009 based on Pearson s Composition and Analysis of Foods 9th Edition, Ronald S Kirk and Ronald Sawyer (page 198). Official Journal Of The European Union, COMMISSION REGULATION (EC) No 152/2009 Using HPLC. Edition 12, 24/10/ T Page 17 of 23
18 Chemical Testing Laboratory Meat and meat Nuts and nut, DETERMINATION OF NITROGEN (PROTEIN) IN FOOD Range: g/100g (%) FCTM010 AOAC Official Method 992. ( , Chapter 39, p.7). Using Leco Nitrogen Analyser. Meat and meat DETERMINATION OF FATTY ACID COMPOSITION IN FOODS Range: Saturated fatty Acids Mono unsaturated Fatty Acids Poly unsaturated Fatty Acids 0.1g/100g FCTM011 AOAC Official method ( , Chapter 41, p.27). Using GLC. Nuts and nut, Meat and meat, game and DETERMINATION OF TOTAL DIETARY FIBRE IN FOODS Range: g/100g FCTM012 Official AOAC method (32.1, Chapter 32, p.7). Using ANKOM Analyser. Nuts and nut, Edition 12, 24/10/ T Page 18 of 23
19 Chemical Testing Laboratory Meat and meat, game and Nuts and nut, Meat and meat, game and ENERGY CALCULATIONS FOR FOODS CALCULATIONS FOR FOODS FCTM014A Added Water. FCTM014B Apparent Total Meat Content by Stubbs and Moore method. FCTM013 Regulation (EU) No 1169/2011 Of The European Parliament And Of The Council. Pearson s Composition and Analysis of Foods 9th Edition, Ronald S Kirk and Ronald Sawyer, (page 5). Regulation (EU) No 1169/2011 on EU provision of Food Information for consumers Regulation. Ronald S Kirk and Ronald Sawyer - Pearson s Composition and Analysis of Food, 9th Edition. (Chapter 13) Meat and meat - the calculation of meat content, added water and connective tissue from analytical data 2nd Ed 2007 (Campden BRI) Royal Society of Chemistry, AMC technical briefs Meat and Poultry Nitrogen factors 2014 Edition 12, 24/10/ T Page 19 of 23
20 Chemical Testing Laboratory Waters Waters for potable and domestic purposes Sewage Trade wastes Other Waters Determination of Chloride, ammonium, nitrite, total oxidised nitrogen (TON), nitrate, alkalinity, hardness, sulphate, orthophosphate using gallery autoanalyser Alkalinity as CaCO mg/L Ammonia as NH 3 -N mg/l Chloride as Cl mg/L Hardness as CaCO mg/l Nitrite as N 0-10mg/L Orthophosphate as P 0-50mg/L Sulphate as SO mg/L TON 1-110mg/L Nitrate as N 1-100mg/L ECTM001A: Thermo- Scientific Method I.D. ALKBpB, Issue 003, 01 ECTM001B: Thermo- Scientific Method ID AMMDIC, Issue 002, ECTM001C: Thermo- Scientific Method ID CHLOR, Issue 002, ECTM001D: Thermo- Scientific Method ID HAR001, Issue 002, ECTM001E:Thermo-Scientific Method ID NITRI, Issue 002, ECTM001F: Thermo- Scientific Method ID PHOS, Issue 002, ECTM001G: Thermo- Scientific Method ID SULP, Issue 002, ECTM001H: Thermo- Scientific Method ID TON, Issue 002, Edition 12, 24/10/ T Page 20 of 23
21 Chemical Testing Laboratory Waters Waters for potable and domestic purposes Sewage Determination of Electrical Conductivity ECTM002: Standard Methods for the Examination of Water and Waste water, Method 2510, 22 nd Edition, APHA, Trade wastes.99 Other Waters Waters Sewage Determination of Suspended Solids using Gravimetry mg/L ECTM003: Standard Methods for the Examination of Water and Waste water, Method 2540, 22 nd Edition, APHA, Trade wastes.99 Other Waters Sewage Trade wastes Other Waters Determination of 5 day Biochemical demand (BOD) using dissolved Oxygen Meter mg/L O 2 ECTM004: Methods for the Examination of Waters and associated materials, HMSO 1988 ISBN , APHA 21st edition 2005, Standard Methods for the analysis of water and waste water 5210 and 4500-C, HACH user manual DOC , Edition 2, 2013 Edition 12, 24/10/ T Page 21 of 23
22 Chemical Testing Laboratory Waters Sewage Trade wastes Determination of Chemical Oxygen Demand (Dichromate Value) Low-range ( 5-150mg/LO 2 ) Mid-range ( mg/LO 2 ) High-range ( mg/LO 2 ) ECTM005: Standard Methods for the Examination of Water and Wastewater, APHA, Method 5220 D, 22 nd Edition, Others Waters 766 Waters Waters for potable and domestic purposes Determination of ph using Orion Star A211 ph 4-10 Ectm007: Standard Methods for the Examination of Water and wastewater, Method 4500, 22 nd Edition, APHA, Sewage.05 Trade wastes.99 Other Waters Edition 12, 24/10/ T Page 22 of 23
23 Chemical Testing Laboratory Waters Sewage Trade waters Determination of Total Phosphorus mg/L P ECTM008: Standard Methods for the Examination of water and wastewater Method 4500-E, 22 nd Edition, APHA, 2012, and HACH method 8190 Edition, 8, 04/ Other Waters 766 Waters.04 Sewage.05 Trade waters.99 Other waters Determination of Total Nitrogen and Kjeldahl Nitrogen Total Nitrogen 1.0 to 200 mg/l N Kjeldahl Nitrogen (Calculation) ECTM006: Method in HACH water analysis Handbook Edition 12, 24/10/ T Page 23 of 23
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