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1 Buffers for Life Science Research CellPURE Biological Buffers JustPURE Good Buffers Core Essential Buffers Quality. Consistency. Selection.

2 CellPURE Biological Buffers performance and reliabilty Fisher BioReagents buffers meet the needs of every budget and scale. of easy dilution Obtaining optimal results in your research requires careful selection of reagents. When your experiments require exact buffering conditions, The purpose of a buffer in a biological system is to maintain intracellular and extracellular ph within the physiological range and resist changes in ph due 1966, these organic buffers have pka values at or near physiological ph and are ideal for cell cultivation, isolation of cells, enzyme assays, and other biochemical applications. Advantages a values mostly independent of temperature and concentration Applications Properties of CellPURE Biological Buffers Product Description Formula Molecular Weight pk 25º C Useful ph Range Cell Culture Tested Endotoxin Assay Nuclease and Protease Tested BP BP BP BP BP BP H H 26 2 O H H H to g pk a = 9.0 for the second dissociation stage Technical Tip The Water Makes a Difference.. 1

3 JustPURE Good Buffers Zwitterionic buffers possessing both positive and negative charges were buffers have several advantages compared to traditional inorganic buffering only trace amounts of metal ions, which makes them useful for applications requiring tight control of elemental content. Applications Advantages Properties of JustPURE Buffers Product Description CAS No. Formula Molecular Weight pk 25º C Useful ph Range Form BP BP BP BP BP BP BP BP BP BP BP BP Tricine H O 4 H H 18 2 O H 26 2 O H H H H 8 2 O 3 H 6 8 H 3 9 H to to g 50g 25g 50g 50g pk a = 9.0 for the second dissociation stage Place your order today! 2

4 Additional Buffers from Fisher BioReagents Choose Fisher BioReagents Buffers for: QUALITY: tight specifications CONSISTENCY: lot-to-lot uniformity SELECTION: powders, concentrated stock solutions, or ready-to-use liquids PACKAGING: state-of-the-art containers designed for safety and utility CONVENIENCE: pre-qualified for a variety of applications ECONOMY: configurations to meet all budgets SCALE: from bench to batch sizes Fisher BioReagents buffers meet the needs of every budget and scale. dilution Suitable for Life Science applications: Obtaining optimal results in your research requires careful selection of reagents. When your experiments require exact buffering conditions, you buffer sterilization is done by 0.2μm fi ltration and/or autoclaving. Description Buffers for Protein Electrophoresis TG Tris-Glycine 10 1L BP L BP L** BP TGS Tris-Glycine-SDS 10 1L BP L BP L* BP L** BP SDS Sodium Dodecyl Sulfate 10% 200mL BP % 1L BP % 200mL BP % 1L BP White Powder BP White Powder 500g BP White Powder 5kg BP166-5 Buffers for Nucleic Acid Electrophoresis TBE Tris-Borate-EDTA 1 1L BP L BP L BP L* BP L BP L BP L BP L** BP TAE Tris-Acetate-EDTA 1 4L BP L BP mL BP L BP L BP L BP L BP mL BP L BP L BP L BP L** BP MOPS Buffer Powder BP Powder 500g BP Buffer Solution 500mL BP Buffer Solution 1L BP Buffers for Hybridization of Nucleic Acids SSPE Saline-Sodium Phosphate-EDTA 20 1L BP L BP L BP SSC Saline-Sodium Citrate 20 1L BP L BP L BP

5 Choose Fisher BioReagents buffers for: QUALITY: tight specifi cations CONSISTENCY: lot-to-lot uniformity SELECTION: powders, concentrated stock solutions, or ready-to-use liquids PACKAGING: state-of-the-art containers designed for safety and utility Description Buffers for Life Science Applications EDTA Ethylenediamine Tetraacetic Acid 0.5M (ph 8.0) 100mL BP M (ph 8.0) 500mL BP M (ph 8.0) 1L BP M (ph 8.0) 20L BP M (ph 8.0), DEPC 100mL BP M (ph 8.0), DEPC 500mL BP M (ph 8.0), DEPC 1L BP Powder 500g BP HEPES 1.0M (ph 7.3) 100mL BP M (ph 7.3) 500mL BP M (ph 7.3) 1L BP299-1 Crystals BP Crystals 500g BP Crystals 1kg BP310-1 Crystals 5kg BP310-5 PBS Phosphate Buffered Saline Tablets 100 tablets BP L BP L BP mL BP L BP399-1 CellPURE PBS 10 4L BP L BP L BP Powder Concentrate 10L BP Powder Concentrate 50L BP Powder with Tween pouches BP Powder with BSA 10 pouches BP Powder Concentrate 2x1L BP665-1 STE Sodium Chloride-Tris-EDTA 1 (ph 8.0) 1L BP (ph 8.0) 1L BP TBS Tris-Buffered Saline 1 (ph 7.4) 1L BP (ph 7.4) 100mL BP (ph 7.4) 1L BP Quality. Consistency. Selection. CONVENIENCE: pre-qualifi ed for a variety of applications ECONOMY: size confi gurations to meet all budgets SCALE: from bench to batch sizes Description TE Tris-EDTA 1 (ph 7.4) 100mL BP (ph 7.4) 500mL BP (ph 7.4) 1L BP (ph 8.0) 100mL BP (ph 8.0) 500mL BP (ph 8.0) 1L BP (ph 7.6) 100mL BP (ph 7.6) 500mL BP (ph 7.6) 1L BP (ph 7.4) 1L BP (ph 7.6) 100mL BP (ph 7.6) 500mL BP (ph 7.6) 1L BP (ph 8.0) 1L BP (ph 8.0) 4L BP L** BP Tris Buffer 0.3M 500mL BP M 1L BP M 100mL BP M 500mL BP Tris Hydrochloride 1.0M (ph 7.0) 100mL BP M (ph 7.0) 500mL BP M (ph 7.5) 100mL BP M (ph 7.5) 500mL BP M (ph 8.0) 100mL BP M (ph 8.0) 500mL BP Solid 500g BP Solid 1kg BP153-1 Tris Base Crystals 500g BP Crystals 1kg BP152-1 Crystals 5kg BP152-5 Crystals 10kg BP Crystals 25kg BP Water Biotech Grade 4L BP L BP Nuclease-Free, Sterile 50mL BP mL BP DNA Grade, Sterile 1L BP RNA Grade, DEPC-treated, Sterile 1L BP561-1 * Pre-weighed powder in poly bottle. Dissolve in water. ** Pre-weighed powder in foil pack. Dissolve in water. Related Core BioReagents Ethanol, Molecular Biology Grade BP BP BP mL 500mL 4L Isopropanol, Molecular Biology Grade BP mL BP L BP L BP L Water (0.1μm filtered), Molecular Biology Grade BP BP BP BP BP mL 1L 4L 10L 20L 4

6 Global Locations To place an order, contact your local Fisher Scientific office Thermo Fisher Scientifi c Inc. All rights reserved. All other trademarks are the property of Thermo Fisher Scientifi c Inc. and its subsidiaries. AMERICAS Canada Fisher Scientific Canada Tel: Fax: Latin America Fisher Scientific Global Export, Latin America Tel: Fax: United States Fisher HealthCare Tel: Fax: Fisher Scientific Tel: Fax: EUROPE Austria info.austria@thermofisher.com Tel: Fax: Germany info.germany@thermofisher.com Tel: Fax: Spain es.fisher@thermofisher.com Tel: Fax: Belgium be.fisher@thermofisher.com Tel: Fax: Ireland fsie.sales@thermofisher.com Tel: Fax: Sweden fisher.se@thermofisher.com Tel: Fax: Czech Republic info.cz@thermofisher.com Tel: Fax: Italy it.fisher@thermofisher.com Tel: Fax: Switzerland info.ch@thermofisher.com Tel: Fax: Denmark kundeservice@thermofisher.com Tel: Fax: The Netherlands nl.info@thermofisher.com Tel: Fax: United Kingdom fsuk.sales@thermofisher.com Tel: Fax: Finland fisher.fi@thermofisher.com Tel: Fax: Norway fisher.no@thermofisher.com Tel: Fax: France fr.fisher@thermofisher.com Tel: Fax: Portugal pt.fisher@thermofisher.com Tel: Fax: ASIA India Korea qfc.customercare@thermofisher.com sales.fsk@thermofisher.com Tel: Tel: Fax: Fax: Malaysia enquiry.my@thermofisher.com Tel: (603) Fax: (603) Singapore enquiry.sg@thermofisher.com Tel: (65) Fax: (65) OCEANIA Australia AUinfo@thermofisher.com Tel: Fax: New Zealand NZinfo@thermofisher.com Tel: Fax: _1728 LW/JG 10/14 BN

Buffers for Life Science Research CellPURE Biological Buffers JustPURE Good Buffers Core Essential Buffers. Quality. Consistency. Selection.

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