TOULOUSE WHITE BIOTECHNOLOGY
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1 Version TOULOUSE WHITE BIOTECHNOLOGY AN ORIGINAL PUBLIC/PRIVATE INTERFACE : from strain engineering to process development Michel MANACH 10th Workshop on Bioeconomy Campinas, 2017 November the 28th,
2 MISSION ACCELERATE DEVELOPMENT IN INDUSTRIAL BIOTECHNOLOGY > Improve the development of new methods of sustainable production through the use of renewable carbon and innovative biological tools (enzymes, microorganisms) > Facilitate the public research / industry interface 1
3 TWB: A PREINDUSTRIAL DEMONSTRATOR Award-winner of Investment for the Future projects call in March 2011 Based on a public / private consortium: constituent assembly meeting on February 2012 Located on its facilities on Sept in Toulouse Administrated by INRA joint service unit (INRA/INSA/CNRS) Certified by the Carnot Institute 2
4 TWB FUNDING Grant from the National Investment Program & ANR > 10M equipment > 10M running costs Fees from the partners of the consortium > # 1M / year Contracts (projects, services) Resources from INRA, INSA, CNRS Other local public funding 3
5 TWB CONSORTIUM A network of 53 partners on January 1 st, 2017 > 38 private companies, 6 investors & tech transfer companies, 9 public bodies A facilitated private / public way of working > accelerate the development and implementation of research projects A consortium agreement > predefined rules of confidentiality, publications and IP > Strategic Steering Committee (SSC) A strategic role > orientations for TWB > choice of research projects 4
6 PARTNERS OF THE CONSORTIUM Partenaires industriels Partenaires investisseurs / valorisation Partenaires publics 5
7 PROJECTS TYPOLOGY PROJECT TYPE Pre-competitive Competitive + technical services Intermediate Objective Generate innovative and fundamental discoveries with potential strong economic benefits Meet the industrial needs, and set up appropriate research programs Set up collaborative research project involving multi public/private partners Private funding 0 % 100 % > 50% IP Public Private Co-property Coaching Public/Private Public/Private Public/Private Valorisation Patents Start-up creation Competitive projects Patents Industrial developments Milestone payments Patents Industrial developments Royalties Results 7 patents 1 start-up 6.4M invested 2 industrial projects 5 patents 3 industrial developments 19.5M signed contracts * 25M success primes negociated 1 patent 1 industrial pilote on the go * Est. end
8 EXAMPLE OF THE PRECOMPETITIVE PROJECT CARBOYEAST 2012 TWB call to precompetitive projects Selection by the SSC* of the Carboyeast project: Capture of CO 2 using yeast Realization of the project by LISBP** teams 3 patents months of exclusivity for the TWB partners November 2015: a start-up is created by Sofinnova Partners TWB signs a two-year exclusive research contract ( 3.3M) with EnobraQ and hosts the start-up (20 persons) in its facility *SSC: Strategic Steering Committee **LISBP : Laboratoire d Ingénierie des Systèmes Biologiques et des Procédés (Toulouse, France) 7
9 ENOBRAQ 8
10 PROJECTS TYPOLOGY PROJECT TYPE Objective Private funding IP Coaching Valorisation Pre-competitive Generate innovative and fundamental discoveries with potential strong economic benefits 0 % Public Public/Private Patents Start-up creation Competitive projects Results 7 patents 1 start-up 6.4M invested 2 industrial projects * Est. end
11 EXAMPLE OF THE COMPETITIVE PROJECT AMOEBA Contributions from TWB: > Microbiology and fermentation expertise > Scale-up from laboratory to production > Reactivity and flexibility 10
12 INTERMEDIATE PROJECT 1: SYNTHACS Production of chemical synthons from biomass by synthetic biology Microorganism Metabolic engineering Cell factory Industrial pilot Competitive price food additives Platform molecules 11
13 INTERMEDIATE PROJECT 2: THANAPLAST A new generation of Bioplastics, using recycled raw materials with programmed biodegradation bpi Microorganism Metabolic engineering Cell factory Industrial pilot Bioplastics with programmed end of life Natural biodiversity Enzymatic engineering Optimized enzymes Classic plastics 12
14 CARBIOS THANAPLAST Discovery of new original enzymes for PLA and conventional plastic (PET ) degradation Creation of a joint venture with Limagrain (European leader in the production of mulching films). Carbios received 8M in 2016 First success fees received by TWB in 2016 ( 250k) 13
15 TWB TECHNICAL PLATFORMS ETHICS ANALYTICAL ENVIRONMENTAL EVALUATION BIOINFORMATICS 14
16 STRAIN ENGINEERING FACILITY 1.56M investment Specificities: - Automated strain engineering station - DNA preparation, Cloning & transformation Main equipment Automated cloning station Liquid transfer station in sterile conditions Nucleic acid extraction station Colony picking station for phenotypic screening Capillary electrophoresis Automated microplate reader NGS sequencer 15
17 ANALYTIC PLATFORM 1.45M investment Specificities: - Open platform for hosted projects - High Sensitivity Mass Spectrometry Main equipment µhplc/uv/ri/cad/dad GC/MS HPLC/MS HPLC/MS/MS SEC/MALS 16
18 CULTURE PROCESS, SCALE-UP AND AUTOMATED CULTURE FACILITY 2.9M investment Specificities: - First world fully automated Culture system Device - Scale up fermentation facility up to 300L Main equipment Robotic station for high-speed fermentation* On-line gas spectrometer analyser Mini parallelised fermenters (500mL) 2L, 5L, 30L fermenters Cytometer analyser Cell sorting cytometer (FEDER co-funding*) 17
19 KEY FIGURES 2016 ( ) collaborators (21 permanent staff) 19 M signed contracts Jan-12 April-12 July-12 Oct patents 79 projects 47 industrial projects 19 precompetitive projects 6 intermediate projects 7 3BCAR projects 18
20 THANKS FOR YOUR ATTENTION Follow TWB 19
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