VWR FOR MICROBIOLOGY ENUMERATION, ISOLATION, DETECTION AND CULTIVATION OF BACTERIA, YEAST AND MOULDS. Dehydrated culture media.

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1 VWR FOR MICROBIOLOGY Dehydrated culture media s Ready to use media Dipslides ENUMERATION, ISOLATION, DETECTION AND CULTIVATION OF BACTERIA, YEAST AND MOULDS Cryopreservation

2 WELCOME TO VWR S GUIDE TO MICROBIOLOGY PRODUCTS Our microbiology portfolio includes dehydrated culture and ready to use media for a wide variety of applications in many different industries, including pharmaceutical, cosmetic, food, dairy, environment and water control. All our media are, as you would expect, produced using high quality raw materials and are subject to strict quality controls, in a production environment following Good Manufacturing Practice guidelines. High quality raw materials are selected for the highest quality products Compliant with International standards, such as ISO and Pharmacopoeia Available in standard formulations and animal-free Custom products available Dehydrated products are available in 500 g and 5 kg packs depending on your usage, and our ready to use media being time and cost savings from convenient, easy to use formulations ranging from plates to bottles, bags and tubes. DOCUMENTATION Documentation is available for download from our website vwr.com for all media products. Technical Data Sheets (TDS) Certificate of Analysis (CoA) in compliance with ISO Standard Material Safety Data Sheets (MSDS) For ready to use media you will also find VHP resistance studies and certificates of irradiation/dosimetry where appropriate. A dedicated technical data sheet is available for every single dehydrated media. Performance testing and Certificates of Analysis of media are in compliance with ISO Standard ISO/TS All batches are strictly checked to guarantee the best results when the media is made up. 2 VWR VWR FOR MICROBIOLOGY

3 VWR MEDIA ARE AVAILABLE IN A CHOICE OF FORMATS FOR MAXIMUM CONVENIENCE Dehydrated media - p g bottle and 5 kg drums Standard formulation and animal-free Ready to use plates - p mm Petri dishes 55 mm Petri dishes for water control (in bags and special RD-Packs) Dehydrated media in bags -p Add only water to prepare liquid media from 50 ml to 10 l Pre-weighed ready to make up bags Sterile: No need for an autoclave Contact slides for surface control - p.32 Perfect for the food industry Ready to use media in bottles - p Contact plates for surface and air control - p Special irradiated packaging options Blister with single plate packaging or Special RD-Packs Tubes with liquid media - p Culture media, ready to use in bags - P. 29 Microbiology media in bags are ideally suited for high sample volumes. Space saving packaging No risk associated with broken glass bottles Media can be easily and conveniently dispensed using a gravimetric diluter or peristaltic pump VWR FOR MICROBIOLOGY VWR 3

4 CULTURE MEDIA INGREDIENTS The ingredients used to prepare culture media are very important because ultimately the results depend on the quality of these compounds. VWR dehydrated ingredients are specifically selected to ensure consistent and reproducible results. Manufactured under the strictest cgmp standards. DESCRIPTION USE AND 500 G 5 KG Bacteriological agar Solidifying agent used in the preparation of microbiological culture media - Bacteriological grade Casein hydrolysate (acid) Peptone obtained by acid digestion of casein, used in the preparation of microbiological culture media Malt extract Extract obtained by extracting the soluble products from sprouted grain, used for the preparation of mycological culture media Meat extract Extract used in general purpose and diagnostic media preparations Peptone from meat (enzymatic digest of animal tissue) Peptone obtained by enzymatic digestion of selected fresh beef meat, used in the preparation of microbiological culture media Skim milk Spray-dried skim milk used in the preparation of microbiological culture media Soya peptone (enzymatic Peptone obtained by enzymatic digestion of soybean meal, used in the preparation of digest of soybean meal) microbiological culture media Tryptone (peptone from casein) Yeast extract Peptone obtained by pancreatic digestion of casein, used in the preparation of microbiological culture media Aqueous extract of autolysed brewer s yeast used in the preparation of microbiological culture media DEHYDRATED CULTURE MEDIA FOR MICROBIOLOGY G BOTTLES AND 5 KG DRUMS Manufactured under the strictest cgmp standards. s required are shown in this table for information and in full in the following section. DESCRIPTION Baird Parker agar base (ISO) Solid selective culture medium for the screening of staphylococci and the isolation of Staphylococcus aureus from a variety of food and pharmaceutical samples s RPF supplement Egg yolk tellurite sterile emulsion Brain heart infusion broth Liquid medium for the cultivation of fastidious and non fastidious aerobic and anaerobic microorganisms Brilliant green agar Kristensen solid selective medium for the isolation of Salmonella spp. other than Salmonella typhi and Shigella Brilliant green agar modified (Brilliant green phenol red lactose agar) Edel and Kampelmacher solid selective medium for the isolation of Salmonella spp. other than Salmonella typhi Brilliant green bile broth Liquid medium for detection and confirmation of Coliform bacteria in water, wastewater, foods, milk and dairy products Buffered peptone water (ISO) Non selective pre-enrichment liquid medium used in methods for the detection of Salmonella spp. (ISO 6579) and Listeria monocytogenes (ISO ) as well as sample preparation (ISO ) in foodstuffs and water IN COMPLIANCE WITH ISO ISO International Dairy Federation DIN DIN DIN ISO ISO ISO ISO 6340 DIN DIN APHA ISO 4831 ISO 4832 ISO 6579 ISO ISO X X ZL XF X X X X X X X X X X X g kg kg 4 VWR VWR FOR MICROBIOLOGY

5 DESCRIPTION Buffered sodium chloride peptone broth (Ph. Eur., USP, JP) General purpose diluent for microbiological analysis and the dilution of samples of pharmaceutical products Blood agar (base) Medium for preparing blood agar plates and heated blood (chocolate) plates Blood agar no. 2 (base) Nutrient rich medium suitable for the isolation of pathogenic microorganisms Campylobacter blood-free medium base Bolton - MCCDA Blood-free solid medium base for the detection and enumeration of Campylobacter spp. in food and animal feeding stuffs CCD selective supplement Campylobacter Bolton broth base (modified CCDA) Liquid medium base for the enrichment (isolation) of Campylobacter spp. in food and animal feeding stuffs (modified CCDA) Bolton broth selective supplement Campylobacter Karmali medium agar base Blood-free solid medium base for the detection and enumeration of Campylobacter spp. in food and animal feeding stuffs Karmali selective supplement Casein peptone lecithin polysorbate broth Medium used to diluite and neutralise samples of pharmaceutical, cosmetic and row material for the purpose of microbial enumeration Columbia agar base (Ph. Eur., USP, JP) Solid medium base for the preparation of culture media for the cultivation of a variety of fastidious and non fastidious microorganisms IN COMPLIANCE WITH Ph. Eur. USP, JP ISO X ISO 7932 X ISO ISO X ISO AOAC X FDA Ph. Eur. USP, JP Ph. Eur. USP, JP ISO X X X X g kg CCA agar Selective and differential medium for the detection and enumeration of coliforms and E. coli in water samples by MF technique Coliform CV selective supplement CLED agar Cystine, lactose, electrolyte deficient medium, recommended for the isolation and identification of urinary pathogenic bacteria DG 18 agar base (ISO) A basal solid medium for the enumeration of yeasts and moulds in foods and animal feeding stuffs with water activity less than or equal to 0,95 Chloramphenicol Dichloran rose bengal chloramphenicol (DRBC) agar Selective solid medium for the enumeration of yeasts and moulds in foods EC broth (ISO) Medium for the detection of faecal coliforms and enumeration of E. coli in waters and foodstuff Endo les agar Solid medium for detecting and enumerating coliforms in water and foods by the filter membrane method Enterobacteriaceae enrichment broth mossel (Ph. Eur., USP, JP) Liquid medium for selective enrichment of enterobacteriaceae from pharmaceutical products in accordance with the microbial limit testing by harmonised methodology of USP, EP, BP, JP Enterobacteriaceae enrichment broth Mossel Liquid medium for selective enrichment of Enterobacteriaceae in the bacteriological examination of food Fecal coliforms agar (m-fc agar) Solid, selective and differential medium for coliform enumeration by membrane filter technique Rosolic acid selective supplement Fluid thioglycollate medium Solid medium for sterility testing of biologicals and for cultivation of aerobes, anaerobes and microaerophiles Fraser broth base (ISO) Media for the selective enrichment of Listeria spp. by the 2-step method s Fraser selective supplement Fraser half selective supplement ISO X X NL ISO X ISO :2008 X ISO 7251 X APHA Standard Methods for the Examination of Water and Wastewater Ph. Eur. USP JP APHA ISO Ph. Eur. USP JP X X X X g kg X X X ZF g kg ISO X g kg VWR FOR MICROBIOLOGY VWR 5

6 DESCRIPTION Hektoen enteric agar Solid medium for differential and selective isolation of Salmonella and Shigella species from enteric pathological specimens KF streptococcus agar base (m-azide maltose agar KF) Solid medium for the detection and enumeration of Enterococci in water, foodstuffs and other materials TTC 1% solution Lactose TTC Tergitol 7 agar (Chapman TTC agar) Solid medium for the detection of coliforms by membrane filtration in water analysis TTC sterile solution 1% Lauryl sulphate broth (ISO) Medium For the detection and enumeration of coliforms (ISO 4831) and for the enumeration of E.coli (ISO 7251) in food and animal feeding stuffs LB broth (Miller) Liquid medium for general purposes, recommended for molecular genetics studies of Escherichia coli; may be used for routine isolation cultivation of not particularly fastidious microorganisms LB agar (Miller) Solid medium for general purposes, recommended for molecular genetics studies of Escherichia coli; may be used for routine isolation cultivation of not particularly fastidious microorganisms Legionella CYE agar base (ISO) Medium base for the detection and enumeration of Legionella spp. in water according to ISO methods s GVPC supplement BCYE α-growth supplement BCYE α-growth supplement without cysteine Letheen broth modified FDA recommended liquid medium for the primary recovery of stressed microorganisms in the microbial examination of cosmetics Polysorbate 80 Listeria buffered enrichment broth base Liquid medium for the pre-enrichment to Listeria species Listeria FDA FIL/IDF selective supplement Listeria enrichment broth Selective broth for the enrichment of Listeria spp. The antibiotics Cycloheximide, Nalidixic acid and Acriflavin HCl are already included in the dehydrated medium Listeria selective agar according to Ottaviani & Agosti Solid culture medium for the isolation of Listeria spp. and the presumptive identification of Listeria monocytogenes s Listeria enrichment supplement Listeria selective supplement Lysine agar Symthetic and differential medium for the isolation, cultivation and enumeration of wild yeast in the brewery industry MacConkey agar (Ph. Eur., USP, JP) Solid medium for the isolation of enteric pathogens and coliform bacteria and for the detection of E.coli in non sterile pharmaceutical products MacConkey broth Medium for presumptive identification of coliforms from variety of specimens such as water, milk and food Malt extract agar no. 2 Solid medium for the isolation and enumeration of fungi Malt extract broth Liquid medium for cultivation of yeasts and moulds Mannitol egg yolk polymyxin agar base MYP (ISO) - (B. cereus selective agar acc. to Mossel) Solid medium for the enumeration of Bacillus cereus in food and animal feeding stuffs s Bacillus cereus antimicrobic supplement Egg yolk emulsion Mannitol salt agar (Ph. Eur., USP, JP) - (Mannitol salt phenol-red agar) Medium for the selective isolation, cultivation and enumeration of Staphylococci Maximum recovery diluent (ISO) Isotonic diluent for maximum recovery of microorganisms IN COMPLIANCE WITH ISO X AOAC APHA APHA X X ISO X ISO 4831 ISO 7251 APHA X X ZK X g kg g kg ISO X X USP FDA FDA-BAM FIL-IDF FIL-IDF FDA X ZA X X NL X X ISO X Ph. Eur. USP JP ZF NL X X X X ISO X X ISO 7932 ISO Ph. Eur. USP, JP X X X X ISO 6887 X VWR VWR FOR MICROBIOLOGY

7 DESCRIPTION M-broth Medium for detecting Salmonella in foods and feeds by the accelerated enrichment serology procedures Membrane lactose glucuronide agar Differential medium for the enumeration of Escherichia coli and other coliforms by the technique of single membrane filtration Milk plate count agar Solid medium for the plate count of milk and dairy products, according to DIN and FIL/IDF standards MRS agar (ISO) Solid medium for the enumeration of lactic acid bacteria from food and animal feeding stuffs (ISO 15214) MRS broth Liquid medium for the enrichment and cultivation of Lactobacillus spp. from all types of materials Muller-Kauffmann tetrathionate-novobiocin broth (MKTTN) (ISO) Selective enrichment liquid medium used for the detection of Salmonella spp. (ISO 6579) s Novobiocin MKTTn supplement Iodine iodide solution Nutrient agar DEV (DEV nutrient agar) For determining the total microbial count in water according to the German Standard Methods and the German regulation for food examination Nutrient agar no.1 (DIN) Solid medium for cultivation of fastidious bacteria Nutrient broth (APHA) Liquid medium for the cultivation of non fastidious microorganisms according to APHA Orange serum agar Solid medium for the culture of aciduric organisms especially those associated with the spoilage of citrus products and their derivatives Oxford agar base Selective and differential solid medium for the detection and isolation of Listeria monocytogenes Oxford supplement Oxytetracycline-glucose-yeast extract agar base (OGYE agar) Solid culture medium for the total enumeration of yeasts and moulds Oxytetracycline selective supplement Palcam agar base (ISO) Selective and differential solid medium for the detection and isolation of Listeria monocytogenes Palcam supplement Plate count agar-pca (ISO) (Standard methods agar) Solid culture medium for the enumeration of microorganisms by the colony count technique Potato dextrose agar (Ph. Eur., USP, JP) Solid culture medium used for the detection and enumeration of yeast and moulds in food, specially recommended for dairy products and other samples and according to the Pharmacopeial harmonised methodology Pseudomonas agar base (ISO) Base solid medium for the isolation and enumeration of Pseudomonas aeruginosa in water samples and Pseudomonas spp. in meat, milk and dairy products s CN selective supplement CFC selective supplement PP selective supplement Glycerol Pseudomonas selective (Cetrimide) agar (Ph. Eur., USP, JP) Solid medium for the selective isolation and differentiation of Pseudomonas aeruginosa Glycerol R2A agar Solid medium for the enumeration of heterotrophic microorganisms in treated waters Rappaport Vassiliadis (RVS) broth Liquid medium for the selective enrichment of Salmonella in foodstuffs and other samples, according to ISO and FIL-IDF standards Rappaport Vassiliadis Salmonella enrichment broth Selective liquid medium used for the enrichment of Salmonella Reinforced clostridial medium A semi-solid medium for the enumeration and cultivation of Clostridia and other anaerobes in food and animal feeding stuffs. Basal medium for the preparation of Differential Reinforced Clostridial Medium IN COMPLIANCE WITH APHA X DIN FIL-IDF ISO X X ISO X X ISO 6579 X X X DIN X APHA X APHA X ISO ISO 7954 ISO 4833 ISO 8552 Ph. Eur. USP JP APHA ISO ISO/TS ISO Ph. Eur. USP, JP Ph. Eur., USP, JP APHA ISO 6579 FIL-IDF Ph. Eur., USP, JP ISO Ph. Eur. USP JP X X NL X X X X g kg X X X X X X X X X X X X VWR FOR MICROBIOLOGY VWR 7

8 DESCRIPTION IN COMPLIANCE WITH Raka Ray agar X Solid selective medium used for the detection and isolation of actic acid bacteria in the beer and brewing process Rose bengal chloramphenicol agar APHA X X X Solid and selective medium for the isolation of yeasts and moulds from the environment and food products Sabouraud 4% dextrose agar Ph. Eur., USP, JP X X Solid medium for the enumeration and cultivation of fungi ISO Sabouraud 2% dextrose broth Liquid sterility test medium for moulds and bacteria in pharmaceutical preparations Selenite cystine broth Enrichment medium for Salmonella spp. from foodstuffs samples SDA with chloramphenicol Medium for the enumeration and cultivation of fungi (yeast and mould) Slanetz and Bartley agar (ISO) For detection and enumeration of intestinal enterococci by membrane filtration method Slanetz Bartley agar (ISO) Differential selective medium for the detection and enumeration of enterococci according to ISO Standard TTC sterile solution 1% SPS agar Medium for detection of Clostridium perfringens in foods TCBS agar Solid medium for the selective isolation of Vibrio parahaemolyticus Tetrathionate crystal violet broth Liquid medium for the selective enrichment of Salmonella spp. from meat and foodstuffs Triple sugar iron agar (ISO) Solid medium for the differentiation of Enterobacteriaceae and for the confirmation test of Salmonella Trytone yeast extract agar (ISO) Solid medium used for the enumeration of water microorganisms according to ISO Standards TSC (Trytose sulphite cycloserine) agar (ISO) Selective and differential medium for isolation and presumptive identification of Clostridium perfringens, according to ISO Standards s D-Cycloserine selective supplement Egg Yolk sterile emulsion Tryptic soy agar (Ph. Eur., USP, JP) (Casein soya bean digest agar) General growth medium for isolation, enumeration and cultivation of microorganisms Tryptic soy broth, non animal origin irradiated, triple wrapped A gamma irradiated sterile medium, recommended for the evaluation of sterility in manufacturing process Tryptic soy agar + lecithin + Tween 80 Solid medium for for environmental monitoring and for the detection of effectiveness of sanitisation procedures Tryptic soy broth A general purpose liquid medium used for cultivation of a wide variety of microorganisms and sterility testing Tryptic soy broth non animal origin TSB non animal origin used for cultivation of a wide variety of microorganisms and sterility testing of moulds and lower bacteria Tryptic soy broth non animal origin irradiated TSB non animal origin gamma irradiated sterile powder recommended for the evaluation of sterility in manufacturing process Tryptic soy broth irradiated TSB gamma irradiated sterile powder recommended for the evaluation of sterility in manufacturing process Tryptone bile x-glucuronide agar TBX (ISO) Medium for the enumeration of β-glucuronidase positive E. coli in food and animal feeding stuffs Tryptose sulphite cycloserine (TSC) agar Medium for the enumeration of Clostridium perfringens in food and animal feeding stuffs d-cycloserine supplement Urea agar base Solid medium for the detection of urease Urea 40% solution Ph. Eur., USP, JP ISO ISO 6785 USP APHA X X X X X X ISO X ISO X ISO WHO and APHA ZK X X X ISO 6579 X ISO 5667 X X ISO X X Ph. Eur., USP, JP ISO Ph. Eur. USP JP Ph. Eur., USP, JP ISO FDA Ph. Eur. USP JP Ph. Eur. USP JP Ph. Eur. USP JP Ph. Eur. USP JP NL ZF X X X X g kg X X X X g kg X X X X kg X X g kg ISO X ISO 7937 EN ISO ISO 6785 ISO 6579 X X ZF 8 VWR VWR FOR MICROBIOLOGY

9 DESCRIPTION Violet red bile glucose (VRBG) agar - (ISO) Solid medium for the detection and enumeration of Enterobacteriaceae Violet red bile lactose (VRB) agar (ISO) For the detection and enumeration of coliform bacteria Wort agar General solid medium for the cultivation of fungi Glycerol Wort broth Liquid medium for the production of yeasts suspensions Glycerol XLD (Xylose lysine deoxycholate) modified agar Solid medium for isolation of enteropathogenic species, especially Shigella and Salmonella in food and animal feeding stuffs, according to ISO Standards Yeast extract agar modified according to ISO 6222 Solid medium for enumeration of microorganisms from water according to ISO Yeast extract dextrose chloramphenicol (YGC) agar Solid medium for the selective isolation and enumeration of yeasts and moulds in milk and dairy products Yeast and mould agar Solid medium for the cultivation of fungi and aciduric microorganisms used for applications in brewing industry IN COMPLIANCE WITH ISO X ISO 4832 X ISO 6579 ISO X X X X ISO 6222 X ISO 7954 FIL-IDF DIN X X SUPPLEMENTS FOR DEHYDRATED CULTURE MEDIA DESCRIPTION SUPPLEMENT FOR AMOUNT OF MEDIA YOU CAN PREPARE Bacillus cereus antimicrobic Mannitol egg yolk polymyxin agar base - MYP (ISO) Cat. No vials 500 ml each supplement BCYE α-growth supplement Legionella CYE agar base (ISO) Cat. No vials 500 ml each BCYE α-growth supplement Legionella CYE agar base (ISO) Cat. No vials 500 ml each without Cysteine Bolton broth selective Campylobacter bolton broth base Cat. No vials 500 ml each supplement Bolton CCD selective supplement Campylobacter blood-free medium base Bolton - MCCDA 10 vials 500 ml each Cat. No CFC selective supplement Pseudomonas agar base Cat. No in compliance with 10 vials 500 ml each ISO (meat products) Chloramphenicol DG18 agar base Cat. No vials 500 ml each CN selective supplement Pseudomonas agar base Cat. No in compliance with 10 vials 500 ml each EN 12780; ISO (water) D-Cyloserine selective TSC (ISO) Cat. No vials 250 ml each NL supplement d-cycloserine supplement Tryptose sulphite cycloserine (TSC) agar Cat. No vials 500 ml each Egg yolk emulsion Mannitol egg yolk polymyxin agar base - MYP (ISO) Cat. No ml bottle 2,5 l Egg yolk tellurite sterile emulsion Baird Parker agar Cat. No ml bottle 2 l XF Egg yolk emulsion TSC agar (ISO) ml bottle 1,56 l ZF Fraser half selective supplement Fraser broth to prepare half Fraser broth Cat. No vials 225 ml each Fraser selective supplement Fraser broth Cat. No vials 500 ml each VWR FOR MICROBIOLOGY VWR 9

10 DESCRIPTION SUPPLEMENT FOR Glycerol Pseudomonas agar base Cat. No , Pseudomonas selective (Cetrimide) agar (Ph. Eur., USP, JP) Cat. No , Wort broth Cat. No and Wort agar Cat. No AMOUNT OF MEDIA YOU CAN PREPARE 100 ml bottle Up to 10 l (depending on application) GVPC supplement Legionella CYE agar base (ISO) Cat. No vials 500 ml each Iodine iodide solution Muller-kauffmann tetrathionate-novobiocin broth (MKTTn) (ISO) 50 ml bottle 2,5 l Cat. No Karmali selective supplement Karmali medium base Cat. No vials 500 ml each Listeria enrichment supplement Listeria selective agar according to Ottaviani & Agosti Cat. No vials 500 ml each ZF Listeria selective supplement Listeria selective agar according to Ottaviani & Agosti Cat. No vials 500 ml each NL Listeria FDA FIL/IDF selective Listeria buffered enrichment broth base Cat. No vials 500 ml each NL supplement Listeria enrichment supplement Cat. No ZF Listeria selective supplement Cat. No NL Listeria selective agar according to Ottaviani & Agosti Cat. No Novobiocin MKTTn supplement Muller-Kauffmann tetrathionate-novobiocin broth (MKTTn) (ISO) 10 vials 500 ml each Cat. No Oxford supplement Oxford agar base Cat. No vials 500 ml each Oxytetracycline selective Oxytetracycline-glucose-yeast extract agar Cat. No vials 500 ml each NL supplement Palcam supplement Palcam agar base (ISO) Cat. No vials 500 ml each PP selective supplement Pseudomonas agar base Cat. No in compliance with 10 vials 500 ml each ISO (milk and dairy products) Rosolic acid selective supplement Fecal coliforms agar (m-fc agar) Cat. No ml bottle 10 l ZF RPF supplement Baird Parker agar Cat. No for the isolation of Staphylococcus 10 vials 100 ml each ZL aureus (ISO :1999) TTC 1% solution KF Streptococcus agar base Cat. No ml bottle 3 l TTC sterile solution 1% Lactose TTC Tergitol 7 agar Cat. No and Slanetz Bartley agar (ISO) Cat. No ml bottle Up to 35 l ZK ZK Urea 40% solution Urea agar base Cat. No ml bottle 2 l ZF CULTURE MEDIA, READY TO USE For various microbiological applications. PRE-POURED PLATES 55 MM Packed under cleanroom conditions to avoid contamination Manufactured to formulations compliant with Pharmacopoeias and ISO regulations Special RD-PACK packaging for 55 mm plates protects plates during transport and storage Packaging can be reused to transport the plates after use and is recyclable (PET) Supplied as either: Box of 30 plates containing 3 plastic bags with 3 RD-PACK PET reusable containers. Each RD-PACK package contains 10x 55 mm plates Box of 90 plates wrapped as above MEDIA Bile Aesculin azide agar CCA coliforms chromgenic agar Solid medium for the confirmation and enumeration of enterococci in clinical samples and water samples by the MF technique according to ISO Standard and clinical samples. Solid, selective and differential culture medium for the detection and enumeration of total coliform and E. coli in water samples by the MF technique. X X 3x UA X 3x ZA X 9x ZAMP 10 VWR VWR FOR MICROBIOLOGY

11 MEDIA CCA coliforms chromgenic agar (ISO) Cetrimide (CN) agar Cetrimide agar Chromocult* coliform agar DEV agar Endo LES agar Lactose TTC agar with Tergitol-7 MacConkey agar (Eur. Pharm.) Malt extract agar acidified Mannitol salt agar m-cp agar m-fc agar M-Green agar Nutrient agar Orange serum agar Plate Count Agar (PCA) R2A agar Sabouraud 4% Dextrose agar Solid, selective and differential culture medium for the detection and enumeration of total coliform and E. coli in water samples by the MF technique acc. to ISO Standard Selective solid medium used for the detection of Pseudomonas aeruginosa according to the EN and ISO Standard Solid culture medium for selective isolation of Pseudomonas aeruginosaaccording to the Harmonised Pharmacopoeia monographs and test methods and ISO Standard Solid, selective and differential culture medium for the detection and enumeration of total coliform and E. coli in water samples by the MF technique. Medium for determining the total microbial count in water according to the German Standard Methods, German Drinking Water Regulations and the German Regulation for Food Examination. Medium for detecting and enumerating coliforms in water and foods by the MF technique. Medium for the detection of coliforms by membrane filtration in water analyses according to ISO Standard :2000. Medium used in the detection, isolation and enumeration of Salmonella and coliforms according to Harmonised Pharmacopoeia monographs and test methods and ISO Standard 21150:2006. Medium for detection, isolation and enumeration of fungi, particularly yeast and moulds, also from air and water samples. Selective medium for the isolation of pathogenic staphylococci according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Solid medium for the enumeration and isolation of Clostridium perfringens in water according to European Directive 12767/97. Selective and differential medium for coliform enumeration by the MF technique. Solid selective culture medium for enumeration of fungi according to ISO standard 10718:2002. Specially for brewery industries. Solid culture medium for general purpose use with less fastidious organisms according to 16266:2006 and ISO Standard EN 12780:2002. Solid medium for the culture of aciduric organisms especially those associated with the spoilage of citrus products and their derivatives. Medium for aerobic plate counts by the surface inoculation method (standard Plate Count Agar) according to ISO Standards 4833, 8552 and and IFU No. 6. Solid medium for the enumeration of heterotrophic microorganisms in treated waters according to Harmonised Pharmacopoeia monographs and test methods. Solid medium for the enumeration and cultivation of fungi according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. X 3x ZA X 3x NA X 3x ZA X 9x ZAMP X 3x ZA 9x ZAMP X 3x ZA X 9x ZAMP X 3x ZA X 3x ZA X X X X 3x ZA X X X 3x YA X X 3x ZA X X 9x ZAMP X 3x ZA X 9x ZAMP X 3x ZA X X 3x ZA X X 9x ZAMP X X X 3x ZA X X X 9x ZAMP X 3x ZA X X X 3x ZA X X 3x ZI X X 3x ZA Sabouraud 4% dextrose chloram agar Medium for the enumeration and cultivation of fungi. X X 3x CF Slanetz & Bartley agar Differential selective medium for the detection and enumeration of X 3x ZA enterococci according to ISO Standard :2000. Slanetz & Bartley agar w/o TTC Differential selective medium for the enumeration of enterococci in X 3x ZA water. X 9x ZAMP X X 3x ZA X X 9x ZAMP TGE agar General solid medium for the enumeration of microorganisms by the MF technique. X 3x ZA Tryptic Soy Agar (TSA) General purpose solid medium containing animal and plant peptone according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. X X 3x ZA Tryptone yeast extract agar Medium for the aerobic plate count in water samples according to ISO Standard 6222:1999 requirements. X 3x ZA TSC agar Solid selective and differential medium for isolation and presumptive X 3x AA identification of Clostridium perfringens, according to ISO Standards. WORT agar Medium for cultivation and enumeration of yeast. X 3x ZA *Chromocult is a registered trademark of Merck KGaA, Darmstadt, Germany. This ready to use medium is made using only Chromocult dehydrated media produced by Merck KGaA and Agar. VWR FOR MICROBIOLOGY VWR 11

12 PRE-POURED PLATES, 90 MM WITH IRRADIATED DOUBLE OR TRIPLE WRAP Packed under cleanroom conditions to avoid contamination Manufactured to formulations compliant with Pharmacopoeias and ISO regulations The double or triple irradiated wrapping ensures that the package itself doesn t contaminate the cleanroom as the first wrapper is removed just before entering the clean area Available with certificate of irradiation/dosimetry VHP resistance studies available for all triple wrapped plates Supplied in box with 10 plates packed in a plastic shrink wrapped bag. Each bag contains 2 packs (20 plates), 3 packs (30 plates) or 12 packs (120 plates). For irradiated plates one pack per bag displays an irradiation indicator. DESCRIPTION Single wrapped Bacillus cereus mossel agar Baird Parker agar Baird Parker RPF agar Bile Asculine azide agar Selective solid medium, according to Mossel, for the isolation and identification of Bacillus cereus from food samples according to ISO Standards. Solid selective culture medium for the screening of staphylococci from a variety of samples, according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Solid selective culture medium for the screening of staphylococci from a variety of samples, according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Solid medium for the confirmation and enumeration of enterococci in water by the membrane filtration method according to ISO Standard. X UA X UAMP X UA X UAMP X IM X IMMP X X XA X X XAMP Brilliant green agar Medium for Salmonella isolation. X ZA X ZAMP Campylobacter CCDA Cetrimide agar Chocolate agar, supplemented Chromocult* Coliform agar CLED agar Coliform chromogenic Agar (CCA) Selective plating medium used for the detection and enumeration of Campylobacter spp according to the ISO :2006 Standard. Solid culture medium for selective isolation of Pseudomonas aeruginosa according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Medium for the isolation and cultivation of fastidious microorganisms especiallly N.meningitidis, N gonorrhoeae and Haemophilus sp. Solid, selective and differential culture medium for the detection and enumeration of total coliform and E. coli in water samples by MF technique. Cystine, lactose, electrolyte deficient medium, recommended for the isolation and identification of urinary pathogenic bacteria. Selective and differential medium for the detection and enumeration of coliforms and E. coli in water samples by MF technique. X ZF X ZFMP X ZA X ZAMP X ZF X ZFMP X X ZA X X ZAMP X ZA X ZAMP X ZA Colorex Rambach agar Differential culture medium for non typhi Salmonella sp. X X ZA X X ZAMP Colorex Salmonella plus Columbia blood agar Chromogenic medium for isolation of Salmonella spp. (ISO Standard 6579:2002). General purpose medium for isolation and culture of microorganisms. The formula of the base complies with requirements included in Harmonised Pharmacopoeia monographs and test methods. X ZA X ZAMP X ZF X ZFMP *Chromocult is a registered trademark of Merck KGaA, Darmstadt, Germany. This ready to use medium is made using only Chromocult dehydrated media produced by Merck KGaA and Agar. 12 VWR VWR FOR MICROBIOLOGY

13 DESCRIPTION Columbia CNA agar CT-SMAC agar Dermatophyte Test Medium (DTM) agar DEV nutrient agar DG 18 metal agar Dichloran Rose Bengal Chloramphenical (RBC) metal agar DNase test agar w. methyl green Eosin Methylene Blue agar (EMB) Eugon LT 100 agar Gauze agar for actinomycete Selective medium from isolation of gram-positive cocci of clinical samples. A selective and differential medium for the detection, isolation and enumeration of E. coli serotype 0157:H7 formulated according to ISO Standard 16654:2001. Selective and differential solid medium used for the isolation and presumptive identification of dermatophytes. Medium for determining the total microbial count in water according to the German Standard Methods. Solid differential and low water activity medium used for the determination of xerophilic fungi in low moisture food and in indoor air supplemented with trace metals. A selective medium for xerophilic moulds and yeast from dried and semi-dried foods. Solid culture medium for the determination of the deoxyribonuclease activity of microorganisms, especially staphylococci and Serratia spp. Selective differential medium for the isolation and enumeration of coliforms according to ISO Standards and USP. Medium for aerobic mesophilic bacteria, in cosmetic products with and without preservatives according to ISO Standard Solid culture medium for the isolation and maintenance of actinomycetes. X NF X NFMP X AA X AAMP X ZA X ZAMP X ZA X ZAMP X XF X XFMP X XF X XFMP X X ZA X ZA X ZAMP X ZF X AF X AFMP GMB - Mueller HInton modified Medium for antibiotic susceptibility testing of yeast and moulds. X DA X DAMP Hektoen enteric agar Lactose TTC agar + Tergitol-7 LB agar (Miller) Lecithin polysorbate Triton X agar Legionella BCYE agar Legionella selective (GVPC) agar Legionella w/o L-Cysteine agar Letheen modified agar Listeria selective agar - Ottaviani Agosti - ISO MacConkey agar no. 3 Malt extract + cloramphenicol agar Malt extract agar Malt extract metal agar Mannitol salt agar MRS agar Solid, selective and differential culture medium for isolation of pathogenic enterobacteria from contaminated samples according to ISO Standard Medium for the detection of coliforms by membrane filtration in water analyses according to ISO Standard :2000. Solid medium for general purposes and recommended for use in molecular genetics studies of Escherichia coli. Universal culture media for a wide spectrum of applications with neutralisers. Solid medium base used for the detection, isolation and enumeration of Legionella from water according to ISO Standards 11731:1998 and :2004. Solid medium base used for the detection, isolation and enumeration of Legionella from water according to the ISO Standards 11731:1998 and :2004. Selective medium for the study of Legionellaceae formulated according to ISO Standard Solid medium for the primary screening of microorganisms in cosmetics according to the F.D.A.. Solid medium for the isolation of Listeria spp. and the presumptive identification of L. monocytogenes. Medium used in the detection, isolation and enumeration of Salmonella and coliforms according to Harmonised Pharmacopoeia monographs and test methods and ISO Standard 21150:2006. Medium for detection, isolation and enumeration of fungi, particularly yeast and moulds, also from air and water samples. Medium for detection, isolation and enumeration of fungi, particularly yeast and moulds, also from air and water samples. Medium for detection, isolation and enumeration of fungi, particularly yeast and moulds, also from air samples according to ISO Standard :2008. Selective medium for the isolation of pathogenic staphylococci according to Harmonised Pharmacopoeia monographs and test methods and ISO Standard 22718:2006. Solid culture medium for the isolation of lactobacilli according to ISO Standards 9332 and and IFU Methods 5, 7 and 9. X ZA X ZAMP X ZA X ZAMP X X ZA X X SA X ZF X ZFMP X GF X XF X XFMP X OA X KA X KAMP X X X X ZA X X X X ZAMP X X CA X X ZA X ZAMP X X XA X X XAMP X X ZA X ZAMP X ZA X ZAMP VWR FOR MICROBIOLOGY VWR 13

14 DESCRIPTION MRS cysteine agar Culture medium for lactobacilli suplemented with cystine. X JA X JAMP MRS cysteine agar 2 X JA X JAMP MRS sorbic acid agar Culture medium for lactobacilli and other acidophilus species. X XA X XAMP Mueller Hinton agar Widely recommended medium for antibiotic and Sulphonamides X ZA susceptibility testing according to the Kirby-Bauer and the Ericsson methods. X ZAMP Mueller HInton blood agar X IF X IFMP Nickerson agar (BiGGY) Culture medium for the isolation and identification of Candida sp. X ZA X ZAMP Nutrient + Bromthymol Blue + TTC Culture medium for general purposes and less fastidious organisms. X PA X PAMP Nutrient agar OGYE agar Orange serum agar Palcam Listeria agar Plate Count Agar (PCA) Potato dextrose agar Pseudomonas CFC agar Rose Bengal Chloramphenicol (RBC) agar Sabourand 4% maltose agar Sabouraud 2% dextrose agar Sabouraud 4% dextrose agar Sabouraud 4% dextrose chloramphenicol agar Sabouraud 4% dextrose chloramphenicol agar Sabouraud 4% dextrose cycloheximide agar Sakazakii agar Salmonella shigella agar Sheep blood polymyxin agar Skirrow selective medium Solid culture medium for general purposes and less fastidious organisms according to ISO Standards 16266:2006 and 12780:2002. Solid medium for detection and enumeration of fungi (yeast and moulds) from food samples according to ISO Standards. Solid medium for the culture of aciduric organisms especially those associated with the spoilage of citrus products and their derivatives. Selective and differential medium for the detection, enumeration and isolation of Listeria sp. Medium for aerobic plate counts by the surface inoculation method (standard Plate Count Agar) according to ISO Standards 4833, 8552 and and IFU No. 6. Medium for yeast and moulds in foods, recommended in dairy products according to Harmonised Pharmacopoeia monographs and test methods. Selective medium for isolation of Pseudomonas spp. in meat and meat products according to ISO Standard Selective medium for isolation of yeast and moulds from the environment and food products. Medium for the enumeration and cultivation of fungi according to USP and EP. Solid medium for the enumeration and cultivation of dermatophytes. Solid medium for the enumeration and cultivation of fungi according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Medium for the enumeration and cultivation of fungi (yeast and mould). Medium for the enumeration and cultivation of fungi (yeast and mould). Medium for the enumeration and cultivation of patogenic fungi (dermatophytes). A solid medium, selective and differential for the presumptive isolation of C. sakasakii in samples of milk and dairy products, according to ISO/TS Differential and selective solid medium for the isolation of Salmonella and some Shigella species from clinical specimens, foods, etc. General purpose medium for isolation and culture of microorganisms. Solid selective culture medium for the screening of staphylococci from a variety of samples according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. X X ZA X X ZAMP X ZA X ZAMP X ZA X ZAMP X ZA X ZAMP X X X ZA X X X ZAMP X ZA X ZAMP X ZA X ZAMP X ZA X ZAMP X ZA X ZAMP X ZA X X ZA X X ZAMP X X CF X X CFMP X DA X DAMP X ZF X ZFMP X ZA X ZAMP X DF X DFMP X AF X AFMP 14 VWR VWR FOR MICROBIOLOGY

15 DESCRIPTION Slanetz and Bartley agar SPS agar TBX agar TCBS cholera agar (ISO :2007) Differential selective medium for the enumeration of enterococci in water formulated according to ISO Standard :2000. Medium for the detection of Clostridium sulphite-reducing in food and water. Selective medium for enumeration and detection of E. coli in foods amd water formulated according to ISO Standard :2001 Solid medium for the selective isolation of Vibrio parahaemolyticus according to the ISO Standards. X ZA X ZAMP X ZA X ZA X ZAMP X ZA X ZAMP Thayer Martin agar Selective medium for Neisseria spp. X OF X OFMP Tryptic Soy Agar (TSA) (Eur. Ph.) Tryptic Soy Agar (TSA) + 5% sheep blood Tryptic Soy Agar + cycloheximide TSC agar + egg yolk General purpose solid medium containing animal and plant peptone according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Nutrient rich medium suitable for the isolation of pathogenic microorganisms from clinical specimens. General purpose medium containing animal and plant peptone, supplemented with Cycloheximide to supress overgrowth of filamentous fungi. Medium recommended for isolation, cultivation and enumeration of Clostridium perfringens according to UNE EN and UNE EN X X X ZA X X ZAMP X IF X IFMP X X AF X X AFMP X AA X AAMP TSN agar Solid selective medium for Clostridium perfringens isolation. X X ZA X X ZAMP Violet Red Bile agar (VRB) Violet Red Bile glucose agar XLD agar - according to ISO 6579 XLD agar (EP) XLD novobiocin agar XLT-4 agar Yeast Glucose Chloramphenicol agar (YGC) Double wrapped Plate Count Agar (PCA) R2A agar Rose Bengal Chloramphenicol (RBC) penase agar RPMI agar Tryptic Soy Agar (TSA) + natamycin agar Selective and differential agar for the detection and enumeration of coliforms. Selective solid medium for the enumeration of enterobacteria Harmonised Pharmacopoeia monographs and test methods and ISO Standard Medium for isolation of enteropathogenic species, especially Shigella and Salmonella in food and animal feeding stuffs according to ISO Standards. Solid medium for the isolation of enteropathogenic species, especially Salmonella and Shigella according to Harmonised Pharmacopoeia monographs and test methods and ISO Standard Medium for isolation of enteropathogen species, specially Salmonella in food and animal feedings stuffs, according to ISO Standard 6579:2002. Solid culture medium for the selective and differential isolation of Salmonella spp. (except S.typhi and S.paratyphi). Solid and selective medium for the isolation and enumeration of fungi in milk and dairy products according to ISO 7954 and FIL-IDF 94B Standards. Medium for aerobic plate counts by the surface inoculation method (standard Plate Count Agar) according to ISO Standards 4833, 8552 and and IFU no. 6. Medium for the enumeration of heterotrophic microorganisms in treated waters. Solid culture medium for the determination of the deoxyribonuclease activity of microorganisms, especially staphylococci and Serratia spp. Solid culture media recommended for the antifungal susceptibility test with rapid growing yeast and moulds (Candida spp. and Cryptococcus neoformans). General purpose medium containing animal and plant peptone, supplemented with Natamycin to supress overgrowth of yeast and mould. X ZA X ZAMP X ZA X ZAMP X ZF X X ZF X X ZFMP X X AF X ZA X ZAMP X CA X CAMP X X X ZI X X X ZIMP X X ZI X X ZIMP X PY X PYMP X ZF X X NF X NFMP Wort agar - TLH Medium for cultivation and enumeration of yeast. X TA X TAMP VWR FOR MICROBIOLOGY VWR 15

16 DESCRIPTION Double wrapped - irradiated Colorex S Rambach agar Differential culture medium for non typhi Salmonella sp. X ZA Legionella selective (GVPC) agar Solid medium base used for the detection, isolation and X GF enumeration of Legionella from water according to ISO Standards 11731:1998 and :2004. Letheen modified agar Medium for the primary detection of microorganisms in cosmetics X ZI according to the F.D.A.. X ZIMP Sabouraud 4% chloramphenicol agar Sabouraud 4% dextrose agar Sabouraud agar + disinfectant neutralisers Sabouraud dextrose agar TLH Standard 1 agar Trypic Soy Agar (TSA) (E.P.) Medium for the enumeration and cultivation of fungi (yeast and mould). Solid medium for the enumeration and cultivation of fungi according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Medium for the enumeration and cultivation of fungi according to Harmonised Pharmacopoeia monographs and test methods with neutralisers. Medium for the enumeration and cultivation of fungi, according to Harmonised Pharmacopoeia monographs and test methods General purpose medium for isolation and culture of microorganisms with neutralisers. General purpose solid medium containing animal and plant peptone according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. X CYMP X X CY X X ZI X ZIMP X TI X TIMP X ZA X X TI X TIMP X ZA Tryptic Soy Agar (TSA) X X ZI X X ZIMP Tryptic Soy Agar (TSA) + disinfectant neutralisers Tryptic Soy Agar (TSA) penase Tryptic Soy Agar (TSA) penase + disinfectant neutraliser Triple wrapped R2A agar Sabouraud 4% dextrose agar General purpose medium for isolation and culture of microorganisms with neutralisers. General purpose medium for isolation and culture of microorganisms with Penicillinase. General purpose medium for isolation and culture of microorganisms with Penicillinase and neutralisers. Solid medium for the enumeration of heterotrophic microorganisms in treated waters according to Harmonised Pharmacopoeia monographs and test methods. Solid medium for the enumeration and cultivation of fungi according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. X X TI X X TIMP X PY X PYMP X RY X RYMP X X ZI X X ZIMP X X ZI Sabouraud 4% dextrose chlor. agar Solid medium for the enumeration and cultivation of fungi. X X CY Tryptic Soy Agar (TSA) General purpose solid medium containing animal and plant X ZI peptone according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. X ZIMP Tryptic Soy Agar (TSA) + disinfectant neutralisers Tryptic Soy Agar (TSA) + disinfectant neutralisers + ß-Lactamase Tryptic Soy Agar (TSA) penase + disinfectant neutralisers General purpose medium for isolation and culture of microorganisms with neutralisers. Solid medium for the enumeration and cultivation of fungi according to the Pharmacopeial Harmonised Methods and ISO Standards. General purpose medium for isolation and culture of microorganisms with Penicillinase and neutralisers. X TI X TIMP X RY X RY X RYMP 16 VWR VWR FOR MICROBIOLOGY

17 CONTACT PLATES IN BAGS, IRRADIATED Ideal for monitoring the microbiological contamination of surface and air inside cleanrooms, isolators and Restricted Access Barrier Systems (RABS). Manufactured to formulations compliant with Pharmacopoeias and ISO regulations The double or triple irradiated wrapping ensures that the package itself doesn t contaminate the cleanroom as the first wrapper is removed just before entering the clean area Special RD-PACK packaging can be reused to transport the plates after use and is recyclable (PET) Available with disinfectant neutralisers TLHTh (tween, lecithin, histidine, thiosulphate) neutraliser suitable for microbiological control of surfaces Available with certificate of irradiation/dosimetry VHP resistance studies available for all triple wrapped plates Supplied as 8 RD-PACK PET reusable containers with double or triple hermetic irradiated wrapping. Each RD-PACK package contains 10 plates with a total of 80 plates. DESCRIPTION PACKAGING Plate Count Agar (PCA) Aerobic plate count for environmental control (air and Triple surfaces). irradiated Plate Count Agar (PCA) + disinfectant neutralisers Rose Bengal Chloramphenical (RBC) agar Sabouraud 4% Dextrose Agar (SDA) Sabouraud 4% Dextrose Agar (SDA) + disinfectant neutralisers Sabouraud Dextrose Chloramphenicol agar Tryptic Soy Agar (TSA) Tryptic Soy Agar (TSA) penase Tryptic Soy Agar (TSA) penase + disinfectant neutralisers Tryptic Soy Agar (TSA) + disinfectant neutralisers Violet Red Bile (VRB) agar Aerobic plate count for environmental control of surfaces treated with detergents or disinfectants. Solid and selective medium for the isolation of yeasts and moulds from the environment and food products. Isolation and count of yeasts and moulds from the environment, according to Harmonised Pharmacopoeial monographs and test methods. Isolation and count of yeasts and moulds from surfaces treated with detergents or disinfectants according to Harmonised Pharmacopoeial monographs and test methods. Isolation and count of yeasts and moulds for environmental control (air and surfaces). Aerobic plate count for environmental control (air and surfaces) according to Harmonised Pharmacopoeia monographs and test methods. Aerobic plate count for environmental control of surfaces in antibiotic production plants. Aerobic plate count for environmental control of surfaces treated with detergents or disinfectants antibiotic production plants. Aerobic plate count for surfaces control of surfaces treated with detergents or disinfectants according to Harmonised Pharmacopoeia monographs and test methods. Selective solid medium for the enumeration of Enterobacteria, acccording to ISO Standard and Harmonised Pharmacopoeia monographs and test methods. Triple irradiated Triple irradiated Triple irradiated Triple irradiated Triple irradiated Triple irradiated Triple irradiated Triple irradiated Triple irradiated Double - NOT irradiated X 8x ZI X 8x TI X X 8x ZI X 8x ZI X 8x TI X X 8x CY X X 8x ZI X 8x PY X 8x RY X 8x TI X X X 8x ZA VWR FOR MICROBIOLOGY VWR 17

18 CONTACT PLATES IN BLISTER, IRRADIATED Ideal for monitoring the microbiological contamination of surface and air inside cleanrooms, isolators and RABS. Manufactured to formulations compliant with Pharmacopoeias and ISO regulations The double or triple irradiated wrapping ensures that the package itself doesn t contaminate the cleanroom as the first wrapper is removed just before entering the clean area Plates are packed in packs of 6x 1 single plate packaging per blister Available with disinfectant neutralisers TLHTh (tween, lecithin, histidine, thiosulphate) neutraliser suitable for microbiological control of surfaces Available with certificate of irradiation/dosimetry VHP resistance studies available for all triple wrapped plates Double irradiated packaging consists of 30 plate packs comprise of 5 with one additional cellophane bag. Triple irradiated packaging consists of 24 plate packs comprise of 4 and additional two cellophane bags An irradiation indicator is featured on each blister with irradiation packaging. DESCRIPTION Double wrapped - NOT irradiated Mannitol salt agar (Chapman agar) Selective medium for the isolation of pathogenic staphylococci according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Violet Red Bile agar (VRB) Medium for the detection and enumeration of coliforms in milk and other dairy products according to APHA and ICMSF, FIL-IDF and ISO Standards. Double wrapped - irradiated Baird Parker agar Rose Bengal agar Malt extract agar + chloramphenicol Solid selective culture medium for the screening of staphylococci from a variety of samples according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Solid and selective medium for the isolation of yeasts and moulds from the environment and food products. Medium for detection, isolation and enumeration of fungi, particularly yeast and moulds, also from air and water samples. X X 5x 6 X X X 5x 6 X 5x 6 20x 6 X X X 5x 6 X X X 20x 6 X 5x 6 Malt extract agar X 5x 6 MacConkey agar (Eur. Pharm.) Plate Count Agar (PCA) + disinfectant neutralisers Plate Count Agar (PCA) Cetrimide agar Sabouraud 4% dextrose chloramphenicol agar Sabouraud 4% dextrose agar + disinfectant neutralisers Selective and differential medium for Salmonella and coliforms according to Harmonised Pharmacopoeia monographs and test methods and ISO Standard 21150:2006. General purpose medium with neutralisers for isolation and enumeration microorganisms in surfaces. Medium for aerobic plate counts by the surface inoculation method (standard Plate Count Agar) according to ISO Standards 4833, 8552 and and IFU No. 6. Medium for selective isolation of Pseudomonas aeruginosa on monitorised surfaces according to Harmonised Pharmacopoeia monographs and test methods. Medium for the enumeration and cultivation of yeast and fungi on surfaces. Medium with neutralisers for the enumeration and cultivation of fungi according to Harmonised Pharmacopoeia monographs and test methods on surfaces. X X X 5x 6 X X 5x 6 X X 5x 6 X 5x 6 X X 5x 6 X X 20x 6 X X 5x ZA ZA UA UAMP ZI ZIMP CA ZI ZA TI ZI ZI CY CYMP TI 18 VWR VWR FOR MICROBIOLOGY

19 DESCRIPTION Sabouraud 4% dextrose agar Tryptic Soy Agar (TSA) + disinfectant neutralisers + ß-Lactamase Tryptic Soy Agar (TSA) agar + cycloheximide Tryptic Soy Agar (TSA) + penase Tryptic Soy Agar (TSA) + penase + disinfectant neutralisers Tryptic Soy Agar (TSA) + disinfectant neutralisers Tryptic Soy Agar (TSA) Violet Red Bile (VRB) agar Violet Red Bile (VRB) dextrose agar + disinfectant neutralisers Violet Red Bile (VRB) dextrose agar Triple wrapped - irradiated Plate Count Agar (PCA) + disinfectant neutralisers Plate Count Agar (PCA) Sabouraud 4% dextrose agar + disinfectant neutralisers Sabouraud 4% dextrose agar Tryptic Soy Agar (TSA) + penase + disinfectant neutralisers Tryptic Soy Agar (TSA) + disinfectant neutralisers Tryptic Soy Agar (TSA) Solid medium for the enumeration and cultivation of fungi according toharmonised Pharmacopoeia monographs and test methods and ISO Standards. General purpose medium with neutralisers and beta-lactams inhibitors, for cultivation and enumeration of microorganisms. General purpose medium for total count supplemented with Cycloheximide to supress overgrowth of filamentous fungi. General purpose medium for isolation and culture of microorganisms with penase. General purpose medium for isolation and culture of microorganisms with Penicillinase and neutralisers. General purpose medium with neutralisers, for enumeration of total aerobics on surfaces. General purpose solid medium containing animal and plant peptone and neutralisers according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. General purpose medium containing animal and plant peptone according to Harmonised Pharmacopoeia monographs and test methods. Medium for the detection and enumeration of coliforms in milk and other dairy products according to APHA and ICMSF, FIL-IDF and ISO Standards. Medium for the enumeration of Enterobacteria according to Harmonised Pharmacopoeia monographs and test methods containing neutralisers. Medium for the enumeration of Enterobacteria according to Harmonised Pharmacopoeia monographs and test methods. General purpose medium with neutralisers for isolation and enumeration microorganisms in surfaces. Medium for aerobic plate counts by the surface inoculation method (standard Plate Count Agar) according to ISO Standards 4833, 8552 and and IFU No. 6. Medium with neutralisers for the enumeration and cultivation of fungi, on surfaces according to Harmonised Pharmacopoeia monographs and test methods. Solid medium for the enumeration and cultivation of fungi according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. General purpose solid medium for enumeration of total aerobics in surfaces according to Pharmacopoeial Harmonised Method and ISO Standards General purpose solid medium containing animal and plant peptone according to Pharmacopoeial Harmonised Method and ISO Standards. X X 5x 6 X 5x 6 X 5x 6 X 20x 6 X 5x 6 X 20x 6 X 5x 6 X 20x 6 X X X 5x 6 X X X 20x 6 X X X 5x 6 20x 6 X X X 5x 6 20x 6 X X X 20x 6 X X X 5x 6 X X X 20x 6 X X X 5x 6 X X X 4x 6 X X X 4x 6 X 4x 6 X 4x 6 X 4x 6 X 4x 6 X X 4x ZI RY AF AFMP PY PYMP RY RYMP SI SIMP TI TIMP ZI ZIMP ZAMP TA TAMP ZA TI ZI TI ZI RY TI ZI VWR FOR MICROBIOLOGY VWR 19

20 CULTURE MEDIA, READY TO USE, IN TUBES For various microbiological uses. Compact packaging, glass tubes are fitted with aluminium screw caps Name of the media, batch number and expiry date ink printed on every sinlge tube Supplied in boxes of 20, 50 or 120 tubes. MEDIA Azide broth (Rothe) Medium for the detection and enumeration of enterococci in water. X X 20x 9 ml ZA X X 120x 9 ml ZAMP Brain heart infusion broth General purpose solid medium for fastidious pathogenic X X X 20x 10 ml ZA (BCC) microorganisms. Brilliant green 2% bile broth Liquid medium for the detection and enumeration of coliforms in water X X 20x 5 ml ZA according to ISO Standard 4831:2006. Brilliant green bile broth Brilliant Green 2% bile broth Liquid medium used for the detection of coliforms in water, as recommended by APHA and ISO Standards. Liquid medium for the detection and enumeration of coliforms in water according to ISO Standard 4831:2006. X X 20x 9 ml ZA X X 120x 9 ml ZAMP X 20x 10 ml WA X 20x 10 ml WAMP Buffered peptone water 0,1% General enrichment or diluent. X X X 20x 10 ml ZA X X X 120x 10 ml ZAMP Buffered peptone water Dilution and non selective pre-enrichment liquid medium according to ISO Standards 6579, 6785, 6887 and X X 20x 9 ml ZA Buffered peptone water 0,1% (Eur. Pharm.) DEV nutrient agar E.C. broth Durham tube mm EE Mossel broth Eugon LT100 broth Fluorocult LMX broth modified Fraser broth Giolitti Cantoni broth Glucose agar Diluent for the homogenisation of samples according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Medium for determining the total microbial count in water according to the German Standard Methods. Selective medium for the detection and enumeration (MPN) of enterobacteria, in water and foodstuffs according to ISO Standards. Selective enrichment of all species of Enterobacteriaceae according to Harmonised Pharmacopoeia monographs and test methods. Liquid medium used for the enrichment of aerobic bacteria including E. coli, in cosmetic products with and without preservatives according to ISO Standards. Selective broth for simultaneous detection of coliform bacteria and E. coli in water and food. Broth for the selective enrichment of Listeria monocytogenes according to ISO Standards. Liquid medium used for the recovery and enumeration of low numbers of coagulase-positive staphylococci in foods according to ISO, FIL-IDF and EN Standards. Solid medium for the confirmation of enterobacteria in diverse samples according to ISO Standards. X X X 20x 9 ml ZA X 20x 15 ml ZA X 20x 9 ml ZA X 20x 9 ml ZA X 120x 9 ml ZAMP X 20x 9 ml SA X X 20x 2 ml ZF X 20x 10 ml ZF X 120x 10 ml ZFMP X 20x 19 ml ZF X 120x 19 ml ZFMP X 20x 9 ml ZF X 120x 9 ml ZFMP X 20x 10 ml WF X 120x 10 ml WFMP X 20x 7 ml ZA Kanamycin Aesculin broth Selective medium for the isolation Enterococci. X 20x 9 ml ZA Kligler agar, slope Differential medium for Enterobacteria according to X X 20x 7,5 ml ZA ISO Standard X X 120x 7,5 ml ZAMP Lactose broth Liquid culture medium used as a preliminary test for coliform bacteria, X X 20x 10 ml ZA formulated according to ISO Standard 4831:2006. Lactose sulphite broth Clostridium perfringens selective detection of spores. X 50x 10 ml AX VWR VWR FOR MICROBIOLOGY

21 MEDIA Lauryl sulphate broth Lauryl sulphate broth MUG (Durham) LB Miller broth Letheen broth Selective medium used as a presumptive test for coliforms and preenrichment broth for coliform organisms. Liquid medium used for the detection and enumeration of coliform bacteria according to IDF-FIL 73B and ISO Standards. Liquid medium for general purposes and molecular genetics studies of Escherichia coli. Liquid culture medium used for the determination of germicidal activity coefficients of cationic detergents. X 20x 5 ml ZA X 120x 9 ml ZAMP X 20x 9 ml ZA X X 120x 9 ml XAMP X X 20x 9 ml XA X X 20x 5 ml ZA X 20x 10 ml ZA X 20x 9 ml ZA X 120x 9 ml ZAMP Löwenstein-Jensen medium Culture and differentiation of Mycobacterium species. X 20x 6,5 ml ZA X 120x 6,5 ml ZAMP Löwenstein-Jensen pyruvate X 20x 6,5 ml XA medium Lysine iron agar, slope Differential medium for Enterobacteria. X 20x 7,5 ml ZA X 120x 7,5 ml ZAMP MacConkey broth purple + Durham Mannitol Motility test Maximum recovery diluent M broth Motility Indole Ornithine medium (MIO) MRS agar MRS broth MR-VP medium Mueller Hinton broth + supplement Müller-Kauffmann Tetrathionate broth O/F glucose medium Orange serum agar Orange serum agar acid Liquid medium, for the detection and enumeration of coliforms according to Harmonised Pharmacopoeia monographs and test methods. Medium for the verification of motility and mannitol fermentation in enterobacteria. Widely used isotonic diluent in microbiology for maximum recovery of microorganisms formulated according to ISO Standard Liquid medium for an accelerated cultivation of Salmonella sp. within the enrichment serology procedure. Medium used for the demonstration of motility, indole production and ornithine-decarboxylase activity of enterobacteria. Solid culture medium for the isolation of lactobacilli according to ISO Standards 9332 and and IFU Methods 5, 7 and 9. Culture medium for lactobacilli according to de Man, Rogosa and Sharpe. X X X 120x 9 ml ZAMP X X X 20x 9 ml ZA X X 20x 9 ml ZA X 20x 9 ml ZA X 20x 9 ml ZA X 20x 9 ml ZA X X 120x 9 ml ZAMP X 20x 20 ml ZA X 120x 20 ml ZAMP X 20x 10 ml ZA Culture medium for lactobacilli. X 20x 9 ml ZA Classic liquid medium used for differential tests (Voges-Proskauer and Methyl Red) in Enterobacteriaceae according to ISO and FIL-IDF Standards. Culture medium for lactobacilli according to de Man, Rogosa and Sharpe. Medium used for the selective enrichment of salmonellae according to ISO Standards. Fluid medium used for determining the oxidative and/or fermentative metabolism of Gram-negative bacilli. For the isolation, cultivation and enumeration of acid-tolerant spoilage microorganisms in fruit juice. Culture media for the isolation and enumeration of acid-tolerant microorganisms in fruit juices. X 20x 10 ml ZA X 120x 10 ml ZAMP X 120x 10 ml ZAMP X 20x 10 ml ZA X 20x 10 ml ZA X 20x 19 ml ZA X 120x 19 ml ZAMP X 20x 20 ml IA X 120x 20 ml IAMP Palcam broth Enrichment broth for Listeria sp. X 20x 10 ml ZF Peptone water Substrate with low nutrient capacity, for the detection of indol production in coliform microorganisms according to ISO Standard X 20x 9 ml ZA Peptone water 0,1% General enrichment or diluent. X 50x 9 ml AX Plate Count Agar (PCA) Medium for aerobic plate counts by the surface inoculation method X X X 20x 20 ml ZA (standard Plate Count Agar) according to ISO Standards 4833, 8552 and X X X 20x 15 ml ZA and IFU No. 6. X X X 120x 15 ml ZAMP Rappaport Vassiliadis broth Rappaport Vassiliadis broth (Eur. Pharm.) Liquid medium for the selective enrichment of Salmonella in foodstuffs and other samples, according to ISO and FIL-IDF Standards. Selective liquid medium used for the enrichment of Salmonella according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. X 20x 10 ml ZA X 120x 10 ml ZAMP X 20x 10 ml ZA X 120x 10 ml ZAMP Ringer solution Isotonic solution for cellular suspensions. X X 20x 10 ml ZA X X 20x 9 ml ZA General diluent. X X 50x 9 ml AX RVS broth Salmonella selective enrichment. X 50x 10 ml AX VWR FOR MICROBIOLOGY VWR 21

22 MEDIA Saboraud broth Sabouraud 4% Dextrose chloramphenicol agar Sabouraud + Chloramphenicol + Cycloheximide Sabouraud chloramphenicol agar Saline + 0,1% peptone broth Saline + PolySorbate Selenite broth Liquid medium for fungal isolation and sterility test according to Harmonised Pharmacopoeia monographs and test methods. Medium for the enumeration and cultivation of fungi (yeast, moulds and dermatophites). Medium for the enumeration and cultivation of pathogenic fungi (dermatophites). Medium for the enumeration and cultivation of fungi (yeast, moulds and dermatophites). Isotonic diluent for the maximal recovery of stressed microorganisms according to ISO Standards. Diluent for the homogenisation with neutralisers of food samples according to Harmonised Pharmacopoeia monographs and test methods. Liquid medium used for the enrichment of Salmonella and Shigella from X X 20x 9 ml ZA X 20x 6,2 ml CA X X 20x 6,2 ml DA X X 120x 6,2 ml DAMP X X 20x 15 ml CA X X 120x 15 ml CAMP X 20x 9 ml ZA X 120x 9 ml ZAMP X X 20x 10 ml OA X X 20x 10 ml ZA Selenite cystine broth clinical specimens and other products according to ISO Standards. X X 20x 10 ml ZA X X 120x 10 ml ZAMP Simmons citrate agar Sodium chloride peptone broth + neutralisers SPS agar DC SPS agar (Perfringens selective agar) SPS agar + paraffin (Perfringens selective agar) Solid medium for verifying citrate utilisation by enterobacteria according to the ISO Standard X X 20x 6,2 ml ZA X 120x 6,2 ml ZAMP Microbial contaminants diluent (Ph. Eur. harmonised, ph Fr X) X 50x 9 ml AX Medium for detection, isolation and enumeration of Clostridium perfringens and Clostridium botulinum in food. Medium for the detection of Clostridium sulphite-reducing in food and water. X 20x 10 ml WA X 120x 10 ml WAMP X 20x 10 ml ZA X X 20x 10 ml XA X X 120x 10 ml XAMP ß-Glucuronidase Broth for detection of ß-glucuronidasa. X X 20x 2 ml ZF Broth for detection of ß-glucuronidasa. X X 120x 2 ml ZFMP TAT broth + Tween 20 Culture from highly viscous/gelatinous materials. X 50x 9 ml AX Thioglycollate medium (Harm. Ph.) Fluid medium used for sterility testing according to the Eur. Pharm., USP, FDA, and for the cultivation of microaerophilic and anaerobic organisms. X 20x 9 ml ZA TB Letheen Bacterial activity of quaternary ammonium compounds. X 50x 5 ml AX Todd Hewitt broth Cultivation of streptococci prior to serological grouping. X 20x 10 ml ZA Todd Hewitt + antibiotic Cultivation of streptococci prior to serological grouping or general X 20x 10 ml AA Todd Hewitt + antibiotic purpose medium. X 120x 10 ml AAMP Triple Sugar Iron agar (TSI) Differential medium for the identification of enterobacteria according to ISO Standards 6579, 6785 and X X 20x 7,5 ml ZA Tryptic Soy Agar (TSA) Total aerobic count. X X 50x 15 ml AX Tryptic Soy Broth (TSB) Highly nutrient medium for general purposes (USP, FDA, EP). X X 50x 9 ml AX Tryptic Soy Agar (TSA) slope Universal culture media for a wide spectrum of applications according to Harmonised Pharmacopoeia monographs and test methods. X X 20x 6,2 ml ZA Tryptic Soy Broth (TSB) USP, FDA, Eu. Ph. Tryptic Soy Broth (TSB) + Tween 80,1% Tryptic Soy Broth (TSB) + Tween 0,1% Tryptone water Tryptone yeast extract agar Tryptic Soy Agar (TSA) Tryptic Soy Broth (TSB) + neutralisers Highly nutrient liquid medium for general purpose use according to Harmonised Pharmacopoeia monographs and test methods. Highly nutrient liquid medium for general purpose use according to USP, FDA and Eu. Ph. regulations. Highly nutrient liquid medium for general purpose use according to Harmonised Pharmacopoeia monographs and test methods. Substrate for dilution and non selective enrichments and for the research of indole production in coliforms formulated according to ISO Standard Medium for the aerobic plate count in water samples according to ISO Standard 6222:1999. Universal culture media for a wide spectrum of applications according to Harmonised Pharmacopoeia monographs and test methods. Highly nutrient liquid medium for general purposes with neutralisers formulated according to Harmonised Pharmacopoeia monographs and test methods. X X 20x 10 ml ZA X X 20x 9 ml ZA X X 20x 15 ml ZA X X 120x 15 ml ZAMP X X 20x 10 ml OA X 20x 9 ml OA X 20x 10 ml ZA X 20x 15 ml ZA X 120x 15 ml ZAMP X 20x 15 ml ZA X 20x 20 ml TA X 120x 20 ml TAMP TSC agar Clostridium perfringens isolation (EN ). X 50x 20 ml AX VWR VWR FOR MICROBIOLOGY

23 MEDIA TSN agar Detection and enumeration of Clostridium perfringens. X X 20x 10 ml ZA X X 120x 10 ml ZAMP Urea broth Liquid medium for the detection of urease in enterobacteriae. X 20x 2 ml ZA X 120x 2 ml ZAMP Urea Indole broth Liquid medium for determination of urease and indole test. X X 20x 2 ml XF X X 120x 2 ml XFMP Violet Red Bile Glucose agar Medium for the enumeration of Enterobacteria according to Harmonised X 20x 15 ml ZA (VRBG) Pharmacopoeia monographs and test methods. Violet Red Bile Agar (VRBA) Medium for the enumeration of Enterobacteria X 20x 15 ml ZA YSG broth Yeast Starch Glucose Agar (YSGA) Liquid medium used for the detection and enrichment of Alicyclobacillus, in fruit juices and other acidic foods according to IFU Standard Method No. 12. Solid medium for the detection and isolation of Alicyclobacillus, in fruit juices and other acidic food according to IFU Standard Method No. 12. X 20x 2,5 ml YF X 120x 2,5 ml YFMP X 20x 20 ml YF X 120x 20 ml YFMP CULTURE MEDIA, READY TO USE, IN BOTTLES Ready to use culture media for microbiological test requirements in the food industry, water and environmental control and in the pharmaceutical industry. This range is manufactured from high quality raw materials. All the culture media are manufactured using selected dehydrated bases, and comply with various standards. All media are produced using the latest technology and are subject to strict quality controls carried out by qualified microbiologists. Supplied in glass bottles with different screw caps Certificate of Analysis, which guarantees product conformity DESCRIPTION Injectable caps Bolton broth base Buffered peptone water Buffered peptone water Buffered peptone water Buffered peptone water Buffered peptone water 0,1% Liquid culture medium used for the enrichment of Campylobacter from food samples according to the ISO Standard :2006, after addition of supplements. Liquid medium for enrichment to the dairy product samples used for PCR Real-Time analysis. Diluent and non selective pre-enrichment broth for the homogenisation of food samples formulated according to ISO Standards 6579, 8523 and Dilution and non selective pre-enrichment liquid medium according to ISO Standards 6579, 6785, 6887 and Diluent and non selective pre-enrichment broth for the homogenisation of food samples formulated according to ISO Standards 6579, 8523 and Diluent for the homogenisation of samples for the microbiological examination according to according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. X 10x 250 ml ZF 10x 250 ml AF X X 10x 90 ml ZA X 10x 225 ml ZA X 10x 225 ml ZA X X 10x 200 ml ZA X X 10x 450 ml ZA X 10x 100 ml ZA X 10x 90 ml ZA VWR FOR MICROBIOLOGY VWR 23

24 DESCRIPTION Buffered peptone water 0,1% + Diluent for the homogenisation of samples for the microbiological X 10x 100 ml TA Tween examination according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Egg yolk sterile emulsion Sterile egg yolk emulsion for microbiological media formulation. 1x 100 ml ZF Egg yolk tellurite sterile emulsion Sterile egg emulsion with potassium telurite for Baird Parker 1x 50 ml XF medium preparation according to ISO Standard Egg yolk tellurite sterile emulsion for Sterile egg emulsion with potassium telurite for Baird Parker 1x 100 ml XF 2 l media in total medium preparation according to ISO Standard Fluid D For use as rinsing and/or diluting fluid in membrane filtration for X 10x 400 ml OA the purpose of any microbiological test. Fraser broth Broth for the selctive enrichment of Listeria monocytogenes. X 10x 225 ml ZF Fraser broth, half Liquid culture medium for the enrichment and detection of X 10x 225 ml VF Listeria ssp. according to ISO Standards. FTM + resarzurin (acc. USP) Fluid medium used for sterility testing according to the Eur. X 10x 100 ml ZA Pharm., USP, FDA and for the cultivation of microaerophilic and anaerobic organisms. Haemophilus Test Medium (HTM) Liquid medium to enhance the growth of Haemophilus spp. 10x 100 ml ZF broth Lactose broth Medium for pre-enrichment and detection of enterobacteria and X X 10x 90 ml ZA coliforms in milk and water according to ISO Standards. Letheen broth Liquid culture medium used for the determination of germicidal X 10x 100 ml ZA activity coefficients of cationic detergents. Lethen broth, modified Medium for the primary detection of microorganisms in cosmetics X 10x 90 ml ZA according to the F.D.A. Liquid A 0,1% Diluent for the homogenisation of samples for the microbiological X 10x 100 ml XA examination according to Harmonised Pharmacopoeial monographs and test methods. Diluent for the homogenisation of samples according to 10x 300 ml XA Harmonised Pharmacopoeial monographs and test methods. X 10x 450 ml XA Listeria Enrichment Broth (LEB) Liquid medium for enrichment of Listeria spp. from foods and X 10x 450 ml ZA enviromental samples. Listeria enrichment supplement that enhances the growth of Listeria spp.. 10x 24 ml ZF Maximum recovery diluent Isotonic diluent for the maximuml recovery of stressed X 10x 90 ml ZA microorganisms according to ISO Standards. Peptone water 0,1% For dilution and non selective enrichments in microbiology. 10x 100 ml ZA PVU neutraliser Medium for the primary detection of microorganisms in 10x 45 ml TA cosmetics. Rosolic acid solution 0,1% Additive for use in microbiology. 1x 100 ml ZF supplement for 12 l media in total Sabouraud dextrose broth Liquid medium for fungal isolation and sterility test according to X X X 10x 100 ml ZA Saline peptone broth Harmonised Pharmacopoeial monographs and test methods. X 10x 90 ml ZA TAT broth base w/o Tween Used for cultivating microorganisms from highly viscous or X 10x 90 ml YZ gelatinous materials. Tryptic Soy Broth (TSB) Tryptic Soy Broth (TSB) + 0,07% lecithine and 0,5% Tween Tryptic Soy Broth (TSB) + 0,1% Tween Tryptic Soy Broth (TSB) + 4% Tween 20 and 0,5% Lecitin Tryptic Soy Broth (TSB) + disinfectant neutralisers TTC in 1% sterile solution Highly nutrient liquid medium for general purpose use according to Harmonised Pharmacopoeial monographs and test methods. Highly nutrient liquid medium for general purpose use according to Harmonised Pharmacopoeial monographs and test methods. Highly nutrient liquid medium for general purpose use according to Harmonised Pharmacopoeial monographs and test methods. Highly nutrient liquid medium for general purpose use according to Harmonised Pharmacopoeial monographs and test methods. Highly nutrient liquid medium for general purpose use according to Harmonised Pharmacopoeial monographs and test methods. Reagent to be added as indicator into many microbiological culture media. X X 10x 90 ml ZA X X 10x 100 ml ZA X X 10x 55 ml ZA X X 10x 200 ml ZA 10x 100 ml SA 10x 90 ml 10x 90ml OA TA X 10x 100 ml TA 1x 100 ml ZK Non injectable caps Antibiotic agar 10 Used in the microbiological assays of Colistimethate, Polymyxin 10x 200 ml ZA Antibiotic agar 10 and carboncillin by the agar diffusion technique. 20x 500 ml ZAMP 24 VWR VWR FOR MICROBIOLOGY

25 DESCRIPTION Antibiotic agar A Used in microbiological antibiotic assays using the agar diffusion technique. 10x 200 ml ZA Antibiotic agar control ph 6,0 Microbiological assay of antibiotics. X 10x 100 ml AX Antibiotic medium A X 6x 200 ml AX Antibiotic medium B X 10x 200 ml AX Antibiotic medium E X 6x 200 ml AX Antibiotic test control ph 8,0 X 10x 100 ml AX Baird Parker agar base Selective culture medium for the detection of staphylococci on a X 10x 90 ml ZA variety of samples according to ISO Standard :1999. Staphylococcus isolation, count and identification. X 10x 180 ml ZA Beerens dilution General diluent (samples with inhibitors and cosmetics). X 10x 100 ml ZA 40x 100 ml ZAMP Bile aesculin azide agar Bolton broth (base) Brain Heart Infusion (BHI) broth, modified Buffered peptone water 0,1% + lecithin Diluent for examination of cosmetic products containing various preservative to neutralise preservative systems. Enterococcus isolation, count and identification according to ISO Standard Liquid culture medium used for the enrichment of Campylobacter from food samples according to ISO Standard :2006, after addition of suplements. Nutrient rich medium suitable for the isolation of pathogenic microorganisms from clinical specimens. Medium for Salmonella enrichment from samples with high lipid content or from materials containing preservatives. X 10x 90 ml ZT 40x 90 ml ZTMP X X 6x 200 ml AX x 250 ml ZFMP X 10x 100 ml ZA 40x 100 ml ZAMP 10x 90 ml LA 40x 90 ml LAMP Buffered peptone water Pathogenic Enterobacteriaceae non selective enrichment. X X 9x 900 ml AX Dilution and non selective pre-enrichment liquid medium X X 10x 100 ml ZA according to ISO Standards. Diluent and non selective pre-enrichment broth for the 9x 800 ml ZA homogenisation of food samples according to ISO Standards 6579, 8523 and x 900 ml ZU Diluent for the homogenisation of samples (EP, USP, JP). X 10x 300 ml ZA Buffered peptone water + 10% Diluent for the homogenisation of samples (EP, USP, JP). X 10x 100 ml TA Tween Buffered peptone water 0,1% Diluent for the homogenisation of samples for the microbiological 40x 100 ml ZAMP examination according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Butterfield buffer Buffered diluent for food samples. X X X 10x 100 ml ZA 40x 100 ml ZAMP Casein peptone lecithin Tween 20 Highly nutrient liquid medium with neutralisers. X X 10x 90 ml AX broth Cetrimide agar Pseudomonas aeruginosa isolation, count and identification X 10x 100 ml AX (Ph. Eur.). X 6x 200 ml AX China Blue Lactose agar Solid and differential medium for the enumeration of lactose fermenting microorganisms in the dairy industry. 10x 200 ml ZA Chromocult Coliform agar Solid, selective and differential culture medium for the detection and enumeration of total coliform and E. coli in water samples using the MF technique. X X 10x 200 ml ZH 10x 100 ml ZA 40x 100 ml ZAMP Count agar Microbial count in milk and dairy products. X 6x 200 ml AX Czapek Dox metal agar Fungi culture with nitrate as the sole nitrogen source. X 10x 475 ml XA DEV lactose peptone broth Total count in water acc. to German Standard Methods. X 10x 50 ml ZA 40x 50 ml ZAMP DEV nutrient agar Solid culture medium for general purpose use with less fastidious organisms according to ISO Standards 16266:2006 and EN 12780:2002. X X 10x 100 ml ZA 40x 100 ml ZAMP Total count in water according to German Standard Methods. X 10x 200 ml ZA Medium for determining the total microbial count in water 10x 450 ml ZA according to German Standard Methods for food examination. VWR FOR MICROBIOLOGY VWR 25

26 DESCRIPTION Dextrose Tryptone Agar (DTA) Solid medium for the cultivation of 'flat-sour' canned food X 10x 100 ml ZA spoiling microorganisms. Diluent P.E. + neutraliser Diluent of non sterile products for microbial contaminants. X 6x 190 ml AX EE broth acc. Mossel Selective enrichment of all species of Enterobacteriaceae X 10x 90 ml ZA according to Harmonised Pharmacopoeia monographs and test methods. 40x 90 ml ZAMP Enterobacteriaceae selective enrichment (ISO Standard 21528). X 10x 90 ml AX Enterobacteriaceae selective enrichment (EP, USP, JP). X 10x 90 ml AX Liquid culture medium used for the enrichment of enterobacteria X 10x 100 ml ZA according to Harmonised Pharmacopoeia monographs and test methods. Fluid D For use as rinsing and/or diluting fluid in membrane filtration for 20 x 400 ml OAMP the purpose of any microbiological test. Fraser broth Broth for the selective enrichment of Listeria monocytogenes. 20 x 225 ml ZFMP Fraser broth 1/2 Liquid culture medium for the enrichment and detection of 20x 225 ml VFMP Listeria spp. according to ISO Standards. FTM + Resarzurin Fluid medium used for sterility testing according to Eur. Pharm., 40x 100 ml ZAMP USP, FDA and for the cultivation of microaerophilic and anaerobic organisms. Henkel standard Inactivating solution for test samples with possible inhibitory 10x 90 ml TH effect of microbiological growth. 10x 90ml TH 20x 90 ml THMP Kanamycin aesculin azide agar Solid medium for confirmative detection and isolation of X X 10x 200 ml ZA Lancefield s group D streptococci in food samples according to Mossel et al. Lactose broth Medium for pre-enrichment and detection of enterobacteria and X X 10x 100 ml ZA coliforms in milk and water according to ISO Standards. Lactose broth + neutralising agents Coliforms selective enrichment. X 10x 90 ml AX X 10x 100 ml AX LB broth acc. Miller Highly nutrient medium for general purposes (USP, FDA, EP). X X 6x 100 ml AX Letheen agar Letheen broth, modified Liquid medium for general purposes and molecular genetics studies of Escherichia coli. Solid medium for assays of antimicrobial action of Quaternary Ammonium Compounds according to the F.D.A. Medium for the primary detection of microorganisms in cosmetics according to the F.D.A. Formulation for the determination of bacterial activity of quartenary ammonium compounds. X 10x 100 ml ZA 40x 100 ml ZAMP 10x 200 ml ZA 40x 90 ml 10x 90 ml 40x 100 ml ZAMP OA OAMP Letheen Tween FeCL3 broth Microbial count diluent. X 6x 100 ml AX Liver meat agar Anaerobic microorganisms culture. X 6x 200 ml AX M17 agar MacConkey agar MacConkey broth Malt extract agar Maximum recovery diluent + Tween Milk plate count agar MRS agar MYP agar base Solid selective medium for the enumeration of Streptococcus thermophilus in yoghurt samples. Isolation and enumeration of Salmonella and coliforms according to Harmonised Pharmacopoeia monographs and test methods and ISO Standard 21150:2006. Liquid medium, for the detection and enumeration of coliforms, according to Harmonised Pharmacopoeia monographs and test methods. Medium for the identification of yeasts and moulds in sterility testing. Diluent for the homogenisation of samples for the microbiological examination according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Solid medium for the plate count of milk and dairy products, according to DIN and FIL/IDF Standards. Medium for the isolation and enumeration of microorganisms in milk and dairy products. Culture medium for lactobacilli, according to Man, Rogosa and Sharpe. Selective solid medium according to Mossel, for the isolation and identification of Bacillus cereus from food samples according to ISO Standards. X 10x 100 ml ZA 40x 100 ml ZAMP X X X X 10x 100 ml ZA X X X 10x 100 ml ZA 10x 200 ml ZA 20x 200 ml ZAMP X X TA X 10x 200 ml XA 10x 450 ml XA 10x 450 ml XAMP X 10x 200 ml ZA X 10x 90 ml ZA 40x 90 ml ZAMP 26 VWR VWR FOR MICROBIOLOGY

27 DESCRIPTION OGYE agar Medium for detection and enumeration of fungi (yeast and moulds) formulated according to ISO Standard 13681:1995. X 10x 200 ml ZA Orange serum agar Acid-tolerant microorganisms isolation in fruit juices. X 10x 100 ml IA X 10x 50 ml ZA 40x 50 ml ZAMP Solid medium for the culture of aciduric organisms especially those associated with the spoilage of citrus products and their derivatives. X 10x 200 ml ZA PBPS 4% Tween 0,5% Lecithin Diluent for the homogenisation of samples for the microbiological examination according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. X 10x 90 ml TH 20 x 90 ml THMP PBTL 3% Tween 0,3% Lecithin X 10x 250 ml TA 20x 250 ml TAMP PCA without glucose Total microbial count in water. X 10x 100 ml AX X 6x 200 ml AX Pharmacopoeia diluent (NaCl peptone broth ph 7) Pharmacopoeia diluent (NaCl peptone broth ph 7) Tween 80 1% Pharmacopoeia diluent w. neutralising agent (DNP) Pharmacopoeia diluent w. neutralising agent (DNP), large neck Pharmacopoeia diluent with neutralising agent (DNP) Pharmacopoeia diluent, sodium thiosulphate 5 g/l Plate Count Agar (PCA) Diluent for analysis of non sterile products. X 9x 90 ml AX X 10x 90 ml AX X 12x 300 ml AX Diluent of non sterile products for microbial contaminants. X 6x 200 ml AX Diluent for analysis of non sterile products. X 10x 90 ml AX Medium for aerobic plate counts by the surface inoculation method (standard Plate Count Agar) according to ISO Standards 4833, 8552 and and IFU No. 6. X 10x 90 ml AX X 9x 600 ml AX X X X X 10x 100 ml ZA 10x 450 ml ZA 20x 450 ml ZAMP X X X 10x 200 ml ZA Polysorbate 80 (Tween 80) Additive for culture media in microbiology. 1x 100 ml ZK Potato dextrose agar Culture medium used for the detection and enumeration of X X 10x 200 ml ZA fungi in food, dairy products and other samples according to Harmonised Pharmacopoeia monographs and test methods Preston broth without blood Selective broth for isolation of Campylobacter sp. X 10x 225 ml ZA R2A agar For use in microbiological monitoriring of waters, according to the monographs for Water from European Pharmacopeia 6th edition, supplement 6.3. X 10x 100 ml ZA Rappaport Vassiliadis broth Selective liquid medium used for the enrichment of Salmonella X 10x 90 ml ZA according to Harmonised Pharmacopoeia monographs and test methods ans ISO Standards. 40x 90 ml ZAMP Salmonella enrichment except S. Typhi and paratyphi A (ISO Standard 6579). X 10x 100 ml AX Salmonella selective enrichment (EP 6th Edition). X 10x 100 ml AX RCM agar Clostridia and anaerobes culture and count (Barnes and Ingram). X X 6x 200 ml AX Reinforced Clostridial Medium (RCM) Fluid medium for the cultivation and enumeration of clostridia by the MPN Techniqueaccording to Harmonised Pharmacopoeia monographs and test methods. 10x 100 ml ZA Sabouraud 2% glucose agar Dermatophytes culture (better yeast and mould count and X X X 6x 200 ml AX culture). Sabouraud 2% glucose broth Yeast and mould count and culture (EP, USP, JP). X X 10x 100 ml AX Sabouraud 2% glucose chloramphenicol agar Sabouraud 4% dextrose agar Dermatophytes culture (better yeast and mould count and culture). Solid medium for the enumeration and cultivation of fungi according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards X X 10x 100 ml AX X 10x 400 ml ZA 20 x 400 ml ZAMP X X 10x 450 ml ZH X X 10x 200 ml ZA Sabouraud chloramphenicol agar Medium for the enumeration and cultivation of fungi. 10x 100 ml CA 40x 100 ml CAMP X X X 10x 200 ml CA X X 10x 400 ml CA Sabouraud dextrose agar X X 10x 100 ml ZA VWR FOR MICROBIOLOGY VWR 27

28 DESCRIPTION Saline peptone broth Selenite cystine broth Slanetz & Bartley agar Sodium chloride peptone broth (buffered) Sodium chloride peptone broth + neutraliser SPS agar Isotonic diluent for the maximum recovery of stressed 40x 90 ml ZAMP microorganisms according to ISO Standards. Liquid medium used for the enrichment of Salmonella and X X 10x 100 ml ZA Shigella from clinical specimens and other products according to ISO Standards. Enterococci differential selective count X 6x 200 ml AX (ISO Standard :2000). Diluent for the homogenisation of samples for the microbiological 10x 300 ml ZA examination according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards. Diluent for analysis of non sterile products. X 9x 290 ml AX Solid medium for the detection of Clostridium perfringens in food and water. X 10x 200 ml ZA TBG broth Salmonella selective enrichment. X X 10x 100 ml AX TBX agar Selective medium for enumeration and detection of E. coli in foods amd water formulated according to ISO Standard :2001. X X 10x 100 ml ZA Tetrathionate broth acc. Müller Kaufmann Medium used for the selective enrichment of salmonellae, according to ISO Standards. X X 10x 100 ml ZA Thioglycolate penase broth Fluid medium for sterility assays formulated according to Harmonised Pharmacopoeia monographs and test methods. X 10x 100 ml PF 40x 100 ml PFMP Thioglycolate resazurin broth Anaerobic and microaerophilic culture, isolation and sterility tests. X 10x 90 ml AX TOS agar Medium for the enumeration of bifidobacteria in milk products. 10x 475 ml ZF Tributyrin agar Medium for detection and enumeration of lipolytic X 10x 100 ml ZA Tributyrin agar microorganisms in food stuffs and other materials. 40x 100 ml ZAMP Triptone yeast extract agar Medium for the aerobic plate count in water samples according to ISO Standard 6222: x 200 ml ZA Tryptic Soy Agar (TSA) Tryptic Soy Broth (TSB) Tryptic Soy Broth (TSB) + 0,07% lecithine and 0,5% Tween General purpose medium containing animal and plant peptone, according to Harmonised Pharmacopoeia monographs and test methods. X X 10x 200 ml ZA X X 10x 450 ml ZH 20x 450 ml ZHMP Total aerobic count. 10x 450 ml ZA Highly nutrient liquid medium for general purpose use, formulated according to Pharmacopeial Harmonised Method. Highly nutrient liquid medium for general purpose use, formulated according to Harmonised Pharmacopoeia monographs and test methods. X X 10x 100 ml ZQ 40x 100 ml ZQMP 10x 450 ml ZA 20x 450 ml ZAMP 10x 450 ml SA Tryptic Soy Broth (TSB) + penase 40x 100 ml PFMP Tryptic Soy Broth (TSB) w. X X 10x 100 ml AX pharmacop. neutralisers Tryptone salt broth (maximum recovery diluent) Tryptic Soy Agar (TSA) Tryptone yeast extract agar TSC agar base Violet Red Bile agar (VRB) Indole formation detection. X 10x 90 ml AX X 10x 225 ml AX General purpose solid medium containing animal and plant X X 10x 400 ml ZA peptone according to according to Harmonised Pharmacopoeia monographs and test methods and ISO Standards Medium for the aerobic plate count in water samples according to ISO Standard 6222:1999. Solid selective and differential medium for isolation and presumptive identification of Clostridium perfringens according to ISO Standards. Medium for the detection and enumeration of coliforms in milk and other dairy products according to APHA and ICMSF, FIL-IDF and ISO Standards. Selective solid medium for the enumeration of enterobacteria, acccording to Harmonised Pharmacopoeia monographs and test methods and ISO Standard X 10x 100 ml ZA 10x 250 ml ZA X 10x 100 ml ZA X 10x 200 ml ZA Violet Red Bile Dextrose agar (VRBD) 10x 450 ml ZA 20x 450 ml ZAMP Violet Red Bile Glucose agar (VRBG) X 10x 200 ml ZA Yeast extract agar Medium for the aerobic plate count in water samples according 10x 200 ml ZA to ISO Standard 6222:1999. YGC agar 28 VWR VWR FOR MICROBIOLOGY Solid and selective medium for the isolation and enumeration of fungi in milk and dairy products according to ISO Standard 7954 and FIL-IDF 94B Standards. Selective agar for isolating and counting yeasts and moulds in milk and milk products. 20x 450 ml 10x 200 ml CA ZA X 10x 100 ml AX X 6x 200 ml AX021259

29 CULTURE MEDIA, READY TO USE, IN BAGS Microbiology media in bags are ideally suited for high sample volumes. Space saving packaging No risk associated with broken glass bottles Media can be easily and conveniently dispensed using a gravimetric diluter or peristaltic pump DESCRIPTION Buffered peptone water Pathogenic Enterobacteriacea non selective enrichment. X X 3x 3 l ZB X X 5x 2 l ZB X X 2x 5 l ZB Fraser broth 1/2 Listeria monocytogenes selective enrichment. X 2x 5 l VB X 3x 3 l VB X 5x 2 l VB Fraser broth, double concentrate Liquid culture medium for the enrichment and detection of X 5x 2 l GB Listeria spp. Maximum recovery diluent Isotonic diluent for maximum recovery of microorganisms (ISO X 2x 5 l ZB Standard ). X 3x 3 l ZB Media Fill Test TSB non animal, irradiated Microbiological validation of aseptic filling (Media Fill Test). X X 1x 20 l AX Media fill test TSB vegetal irradiated X X 1x 1 l AX Tryptic Soy Broth (TSB) Highly nutrient liquid medium for general purposes (USP, FDA, PE). COSMETICS X 2x 5 l ZB X 3x 3 l ZB Tryptic Soy Broth (TSB) radio sterilsed Microbiological validation of aseptic filling (Media Fill Test). X 2x 5 l AX Modified Letheen broth + Tween (MLBT) Medium for the primary detection of microorganisms in cosmetics X 3x 3 l ZA according to the F.D.A.. Polyvalent universal neutraliser Medium for the primary detection of microorganisms in X 2x 5 l TA cosmetics. X 2x 5 l TA Accessory for all the bags Connector, stainless steel Connector to bags with prepared liquid medium. X X X X OO Feel the chemistry More than 1000 new products including solvents, standards, microbiology media, microscopy and life science products. Also includes the most important Amresco products. Request your copy at vwr.com or from your local VWR sales office VWR FOR MICROBIOLOGY VWR 29

30 CULTURE MEDIA, MEDIABAG Each bag contains a pre-weighed amount of granulated media in a double bagged bag and is irradiated. Just in time enrichment - add sterile water and sample material. Space saving, no bottles taking up shelf space Long shelf life No autoclaving and optimum solubility Dust-free work condition MediaBag, buffered peptone water (BPW) irradiated Non selective pre-enrichment broth to increase recovery of bacteria, particularly pathogenic Enterobacteriaceae from foodstuffs. The culture medium complies with ISO 6579:2002. The lab-blender bag contains 5,75 g of BPW to which 225 ml of sterile water and 25 g (or 25 ml) of sample material is added. More media are available on request. The following media are approved for the bag system: Tryptic soy broth Fraser broth base Lactose broth MacConkey broth The composition is according to standard methods such as ISO, AFNOR, NMKL etc. Packaging Special formulations and packaging formats available on request. DESCRIPTION VOLUME OF STERILE TO BE ADDED TO EACH BAG Small bag with BPW, irradiated, ready to use MediaBag, BPW sachets 225 ml MediaBag, BPW sachets 225 ml MediaBag, BPW sachets 2700 ml Standbag with BPW, irradiated, ready to use MediaBag, standbag with BPW 90 ml MediaBag,1/2 Fraser with supplement 225 ml MediaBag, standbag skimmed milk 225 ml MediaBag, standbag with BPW 900 ml MediaBag, standbag with BPW 1125 ml MediaBag, standbag with BPW 2250 ml Aluminium standbag with BPW, irradiated, ready to use MediaBag, aluminium standbag with BPW 3000 ml MediaBag, aluminium standbag with BPW 3375 ml MediaBag, standbag with BPW 3375 ml MediaBag, aluminium standbag with BPW 4000 ml MediaBag, aluminium standbag with BPW 4500 ml Large bag with BPW, irradiated, ready to use MediaBag standbag, big bag with BPW 2250 ml MediaBag, big bag with BPW 4500 ml MediaBag, big bag with BPW 6750 ml MediaBag standbag, big bag with BPW 9000 ml Accessories MediaBag standbag empty box 5000 ml VWR VWR FOR MICROBIOLOGY

31 CULTURE MEDIA, MEDIABAG SYSTEM T The T bag is filled with pre-weighed granulated media. Connect the 0,2 μm filter unit aseptically to the tube by using the the connector device. Add water under aseptic conditions into the bag via the filter sytem. Protect the tube end with the red cap. Before and after addition of water to the bag DESCRIPTION VOLUME OF STERILE TO BE ADDED TO EACH BAG MediaBag T (tube-bag) for 3 l BPW (buffered peptone water) 3000 ml MediaBag T BPW (buffered peptone water) 5000 ml MediaBag T BPW (buffered peptone water) ml MediaBag T BPW (buffered peptone water) ml MediaBag T BPW (buffered peptone water) ml MediaBag T maximum recovery diluent 3000 ml MediaBag T maximum recovery diluent 5000 ml MediaBag T maximum recovery diluent ml MediaBag T maximum recovery diluent ml MediaBag T half Fraser ml Fraser broth 1/ ml MediaBag T BLEB with supplemet, no filter ml MediaBag T BPW, no filter ml 600 bag MediaBag T BPW, with filter ml MediaBag T BPW (buffered peptone water) ml MediaBag T BPW (buffered peptone water) ml MediaBag T BPW (buffered peptone water) ml MediaBag T Demi Fraser, no filter ml MediaBag T for BPW, 65 cm tube ml MediaBag T half Fraser 5000 ml MediaBag T TSB 5000 ml CULTURE MEDIA, MEDIABAG SYSTEM W W bags are folded bags without connectors. DESCRIPTION FOR MediaBag W BPW (buffered peptone water) 3600 ml MediaBag W BPW (buffered peptone water) 1800 ml MediaBag W BPW (buffered peptone water) 1125 ml MediaBag W BPW (buffered peptone water) 2700 ml MediaBag W BPW (buffered peptone water) 3000 ml MediaBag W BPW (buffered peptone water) 2250 ml MediaBag W BPW (buffered peptone water) 3375 ml MediaBag W Demi Fraser, sterile, 2250 g 2250 ml MediaBag W Demi Fraser with supplement 900 ml MediaBag W Demi Fraser with supplement 1125 ml Accessories MediaBag filter unit and connector VWR FOR MICROBIOLOGY VWR 31

32 DIP SLIDES Dip slides for microbiological monitoring of surfaces and in liquids. Each slide has media on both sides. Choice of the same medium or two different media on the two sides of the slides; this allows sampling of two surfaces with every single slide. Testing with dip slides is a safe, reliable, economical and fast indicator of the presence of microorganisms. Dip slides with a push cap have a larger contact area. Sampling takes only a few seconds The results are easily read after just hours For food and water control Type Push cap Contact area (cm²) 10 Dimensions 30 mm tube, 110 mm high Tray W L (mm) 50x22 Paddle size (mm) DESCRIPTION Baird Parker Staphylococcus aureus D D Baird Parker/VRBG Staphylococcus aureus/enterobacteriaceae D Malt extract Yeast and moulds D D Nutrient/Pseudomonas Total count/pseudomonas T R Nutrient TTC + neutralisers Total count D D Nutrient TTC Q Nutrient TTC/malt extract Total count/yeast and moulds B D Nutrient + TTC - screw cap Total count D D Nutrient + TTC - large S PCA/MacConkey no. 3 Total count/salmonella, Shigella and coliform J L PCA/Baird Parker Total count/staphylococcus aureus D D PCA/VRBG + neutralisers Total count/enterobacteriaceae P PCA + neutralisers - black Total count D D PCA + TTC D PCA + TTC + neutralisers D D PCA + TTC D PCA F H PCA/VRBG - large Total count/enterobacteriaceae N PCA/VRBG + neutralisers - large R PCA/VRBG P PCA/MacConkey + neutralisers Total count/salmonella, Shigella and coliform L N PCA/OGYE + neutralisers Total count/yeast and moulds Total count/salmonella, Shigella and coliform H J PCA + TTC/MacConkey no D D PCA + TTC/MacConkey no. 3 with neutralisers D D Pseudomonas CFC/MacConkey no. 3 Pseudomonas sp./salmonella, Shigella and coliform D D Rose bengal - large Yeast and moulds E G 32 VWR VWR FOR MICROBIOLOGY

33 DESCRIPTION Tryptic Soy (TSA) + neutralisers Disinfectant control D D Tryptic Soy (TSA) + TTC/rose bengal chloramphenicol Total count/yeast and moulds D F Tryptic Soy(TSA) + TTC/malt extract Total count/yeast and moulds D D PRESERVATION OF MICROORGANISMS, CRYOINSTANT Microbiological laboratories need a simple procedure to maintain important microorganisms rather than repetitive subculturing, which results in contaminated cultures, loss of viability and even more important, loss of original characteristics by introducing biochemical and genetic changes. Cryoinstant consists of 25 porous beads in a vial filled with a broth containing glycerol. Microorganisms will bind to the porous surface of beads. The excess of broth is aspirated, the vials can then be stored up to -70 C in a freezer. Quick and easy to use Colour coded Storage down to -70 C Quality controlled for fertility and sterility Cryoinstant is packed in shelf packs of 50 vials of 2 ml. DESCRIPTION Cryoinstant vials, blue X X X X ZA Cryoinstant vials, green X X X X ZA Cryoinstant vials, mixed X X X X ZA Cryoinstant vials, natural X X X X ZA Cryoinstant vials, red X X X X ZA Cryoinstant vials, yellow X X X X ZA Research tools for life scientists AYN for Genomics AYN for Cell Culture AYN for Proteomics AYN for Biobanking VWR INTERNATIONAL VWR INTERNATIONAL VWR INTERNATIONAL ALL YOU NEED ALL YOU NEED ALL YOU NEED FOR GENOMICS FOR GENOMICSFOR GENOMICS VWR INTERNATIONAL VWR INTERNATIONAL VWR INTERNATIONAL ALL YOU NEED ALL YOU NEED ALL YOU NEED FOR CELL CULTURE FOR CELL CULTURE FOR CELL CULTURE Plasmid purification Plasmid purification Plasmid purification Genomic DNA purification Genomic DNA purification Genomic DNA purification RNA purification RNA purification RNA purification Clean-up Clean-up Clean-up Serum Serum Serum Media Media Media Reagents Reagents & supplements & supplements Reagents & supplements Automated Automated DNA/RNA DNA/RNA Automated DNA/RNA extraction extraction extraction DNA/RNA DNA/RNA storage storagedna/rna storage Modifying enzymes Modifying enzymes Modifying enzymes And more... And more... And more... Transfection Transfection Transfection RNAi RNAi RNAi Electroporation Electroporation Electroporation Tissue culture plasticware Tissue culture plasticware Tissue culture plasticware Instruments & equipment Instruments & equipment Instruments & equipment And more... And more... And more... Available as printed (hard) copy or PDF/flip catalogue via our website vwr.com All You Need guides VWR FOR MICROBIOLOGY VWR 33

34 HYGIENE CONTROL AND DISINFECTION Air samplers Dip slides and contact plates, mini incubators Disinfection control Swabs/transport swabs Alcohol sprays and disinfectants RAPID TESTS Reagents and consumables for molecular biology Thermocyclers (PCR/qPCR) ESSENTIAL EQUIPMENT Autoclaves Analytical balances Data loggers Dispersion equipment Gas burners Homogenisors and blenders Colony counter Incubators and cooled incubators Microscopes and stereo microscopes Magnifiers ph meters Lab blender Sterilisers and ovens Drying incubators Water baths Water purification systems Centrifuges PERSONAL PROTECTION Labcoats and apparel Eyewear Gloves Hand cleaning Footwear 34 VWR VWR FOR MICROBIOLOGY

35 CULTURE MEDIA Dehydrated media Ready to use media, plates, tubes, bottles and bags Irradiated media for Mediafill tests s and additives Custom products Preservation systems CONSUMABLES Akku, pasteur and pipette controllers Autoclave bags Covering up aids Dispensers Disposable sampling bags and containers Durham tubes Inoculation containers from 250 ml to 5 l Lab blender bags Microbiology copying implements Petri dishes, inoculation loops and Digalski spatulas Pistons, tubes, bottles and silicone plugs Slides and cover slips Staining solutions Suction stations and membrane filters Test tubes and stands Vacuum pumps and suction bottles SERVICES Calibration, maintenance and repair IQ/OQ qualification Bypass devices Application technology consultation VWR FOR MICROBIOLOGY VWR 35